DiGeorge syndrome is a disorder caused by a microdeletion on the long arm of chromosome 22. Approximately 1% of patients diagnosed with DiGeorge syndrome may have an absence of a functional thymus, which characterizes the complete form of the syndrome. These patients require urgent treatment to reconstitute T cell immunity. Thymus transplantation is a promising investigational procedure for reconstitution of thymic function in infants with congenital athymia. Here, we demonstrate a possible optimization of the preparation of thymus slices for transplantation through prior depletion of thymocytes and leukocyte cell lineages followed by cryopreservation with cryoprotective media (5% dextran FP 40, 5% Me2SO, and 5% FBS) while preserving tissue architecture. Thymus fragments were stored in liquid nitrogen at -196°C for 30 days or one year. The tissue architecture of the fragments was preserved, including the distinction between medullary thymic epithelial cells (TECs), cortical TECs, and Hassall bodies. Moreover, depleted thymus fragments cryopreserved for one year were recolonized by intrathymic injections of 3×106 thymocytes per mL, demonstrating the capability of these fragments to support T cell development. Thus, this technique opens up the possibility of freezing and storing large volumes of thymus tissue for immediate transplantation into patients with DiGeorge syndrome or atypical (Omenn-like) phenotype. 22 1 immunity athymia Here 5 (5 40 Me2SO MeSO Me SO FBS 196°C 196C C 196 3 preserved TECs , (TECs) bodies Moreover 3106 106 3×10 mL development Thus Omennlike Omenn like (Omenn-like phenotype 2 ( 4 19 (TECs 310 10 3×1 31 3×

Glioblastoma is the most prevalent and malignant brain tumor identified in adults. Surgical resection followed by radiotherapy and chemotherapy, mainly with temozolomide (TMZ), is the chosen treatment for this type of tumor. However, the average survival of patients is around 15 months. Novel approaches to glioblastoma treatment are greatly needed. Here, we aimed to investigate the anti-glioblastoma effect of the combination of matteucinol (Mat) (dihydroxyflavanone derived from Miconia chamissois Naudin) with the chemotherapeutic TMZ in vitro using tumor (U-251MG) and normal astrocyte (NHA) cell lines and in vivo using the chick embryo chorioallantoic membrane (CAM) assay. The combination was cytotoxic and selective for tumor cells (28 μg/mL Mat and 9.71 μg/mL TMZ). Additionally, the combination did not alter cell adhesion but caused morphological changes characteristic of apoptosis in vitro. Notably, the combination was also able to reduce tumor growth in the chick embryo model (CAM assay). The docking results showed that Mat was the best ligand to the cell death membrane receptor TNFR1 and to TNFR1/TMZ complex, suggesting that these two molecules may be working together increasing their potential. In conclusion, Mat-TMZ can be a good candidate for pharmacokinetic studies in view of clinical use for the treatment of glioblastoma.

RESUMO Objetivou-se identificar estratégias de rebaixamento da Urochloa brizantha cv. Marandu no início do diferimento, as quais resultem em aumento da produção e em melhoria da estrutura do dossel diferido. Foram avaliadas três estratégias de rebaixamento no início do diferimento: manutenção do capim-marandu com 15cm por quatro meses antes do início do diferimento; manutenção do capim-marandu com 30cm por quatro mese, e rebaixamento para 15cm no início do diferimento; e manutenção do capim com 45cm por quatro meses e rebaixamento para 15cm no início do diferimento. O delineamento foi inteiramente ao acaso, com quatro repetições. No fim do diferimento, o número de perfilho reprodutivo e a massa de forragem foram superiores no dossel sob 15/15cm do que nos demais; a percentagem de folha viva foi maior no dossel sob 45/15cm do que sob 15/15cm. A produção de forragem durante o diferimento foi superior no dossel sob 15/15cm e inferior no sob 45/15cm. A manutenção do capim-marandu com 15cm por quatro meses antes do período de diferimento aumenta a produção de forragem neste período. O rebaixamento do capim-marandu de 45 para 15cm no início do diferimento melhora a estrutura do dossel diferido.

ABSTRACT The objective was to identify lowering strategies for Urochloa brizantha cv. Marandu in the beginning of the stockpiling period that increase forage production and improve the structure of stockpiled canopies. Three lowering strategies were evaluated: maintenance of marandu palisade grass with 15cm four months before the stockpiling period; maintenance of palisade grass with 30cm for four months, and lowering to 15cm before the stockpiling period; and maintaining of palisade grass at 45cm for four months, and lowering to 15cm before the stockpiling period. The experimental design was completely randomized, with four replications. At the end of stockpiling, the number of reproductive tillers and forage mass were higher in the canopy under 15/15cm than in the others; the percentage of live leaf was higher in the canopy under 45/15cm than under 15/15cm. Forage production during the stockpiling period was higher in the canopy under 15/15cm and lower in the under 45/15cm. Maintaining 15cm marandu palisadegrass for four months before the stockpiling period increases forage production during this period. The lowering of the marandu palisadegrass from 45 to 15cm at the beginning of the stockpiling period improves the structure of the stockpiled canopy.

RESUMO O objetivo deste estudo foi identificar diferenças nos padrões morfogênicos de quatro cultivares de Urochloa brizantha (marandu, piatã, xaraés e paiaguás) durante o período de diferimento. Foi utilizado o delineamento inteiramente ao acaso, com três repetições, em parcelas experimentais de 9m². As avaliações ocorreram por dois anos. Os capins foram diferidos por 92 (Ano 1) e 95 (Ano 2) dias. A taxa de aparecimento foliar do capim-paiaguás foi maior, em comparação aos demais capins. No Ano 1, a taxa de alongamento do colmo do capim-xaraés foi superior aos demais capins. No final do diferimento do Ano 1, a densidade populacional de perfilho (DPP) foi maior no capim-paiaguás, intermediária nos capins xaraés e marandu e inferior no capim-piatã. No fim do período de diferimento do Ano 2, a DPP foi superior no dossel de capim-paiaguás, intermediária nos dosséis dos capins marandu e piatã, e menor no dossel de capim-xaraés. O capim-paiaguás tem maior crescimento foliar durante o período de diferimento, sendo, portanto, apropriado para uso sob pastejo diferido. O capim-xaraés apresenta elevado alongamento de colmo durante o período de diferimento, razão pela qual seu uso sob pastejo diferido deve vir acompanhado de ajustes no manejo da pastagem.

ABSTRACT The objective this study was to identify differences in the morphogenic patterns of four Urochloa brizantha cultivars (marandu, piatã, xaraés and paiaguás grasses) during the stockpiling period. A completely randomized design was used, with three replications, in experimental plots of 9m². The evaluations took place over 2 years. The grasses were stockpiled for 92 (Year 1) and 95 (Year 2) days. The leaf appearance rate of paiaguás grass was higher, compared to other grasses. In Year 1, the stem elongation rate of xaraés grass was higher than other grasses. At the end of stockpiling period of Year 1, the tiller population density (TPD) was higher in the paiaguás grass, intermediate in the xaraés and marandu grasses and lower in the piatã grass. At the end of the stockpiling period in Year 2, TPD was higher in the paiaguás grass canopy, intermediate in the marandu and piatã grasses canopies, and lower in the xaraés grass canopy. Paiaguás grass has greater leaf growth during the stockpiling period and is therefore suitable for use under stockpiled grazing. Xaraés grass has high stem elongation during the stockpiling period, which is why its use under stockpiled grazing must be accompanied by adjustments in pasture management.

Abstract Humic substances are increasingly being used as biostimulants and may even benefit the development of forage peanut (Arachis pintoi cv. Amarillo MG 100), a promising species for the recovery of degraded pastures. The study aimed to evaluate different concentrations of humic substances extracted from vermicompost from cattle manure on growth and nutrient content in forage peanut plants inoculated with Bradyrhizobium spp. The experiment was carried out in a greenhouse, in a randomized block design, with five concentrations of humic substance and five replications. The shoot dry matter, root dry matter, the number of nodules, the content of macronutrients in the shoot, total chlorophyll content, chlorophyll a/b ratio, and quantum efficiency of the photosystem II were evaluated. The addition of humic substances increased the yield and nutrient accumulation of the forage peanut plants. The shoot dry matter responded in a quadratic manner to the addition of humic substances. The concentration of 40 mg L-1 of C increased 45.85% of the number of nodules compared to the control. There were improvements in efficiency of the photosystem II and the accumulation of nitrogen, potassium, calcium and magnesium by up to 12 % (37.1 to 41.5 mg pot-1), 8.6 % (35.3 to 38.3 mg pot-1), 13.5 % (21.8 to 24.7 mg pot-1) and 7.5 % (9.8 to 10.53 mg pot-1), respectively, in the shoot with the humic substances application, except for the phosphorus accumulation.

Resumen Las sustancias húmicas se utilizan cada vez más como bioestimulantes y pueden también beneficiar el desarrollo del maní forrajero (Arachis pintoi vc. Amarillo MG 100), una especie prometedora para la recuperación de pastos degradados. El objetivo del estudio fue evaluar el efecto de diferentes concentraciones de sustancias húmicas extraídas del vermicompost de estiércol de ganado en el crecimiento y contenido de nutrientes en plantas de maní forrajero inoculadas con Bradyrhizobium spp. El experimento se realizó en un invernadero, con un diseño de bloques al azar, cinco concentraciones de sustancia húmica y cinco réplicas. Se evaluó la materia seca del brote, la materia seca de la raíz, el número de nódulos, el contenido de macronutrientes en el brote, el contenido de clorofila total, la relación clorofila a/b y la eficiencia cuántica del fotosistema II. La adición de sustancias húmicas aumentó el rendimiento y la acumulación de nutrientes de las plantas de maní forrajero. La materia seca de los brotes respondió de forma cuadrática a la adición de sustancias húmicas. La concentración de 40 mg L-1 de C aumentó en 45.85 % el número de nódulos en comparación con el control. Hubo mejoras en la eficiencia del fotosistema II y la acumulación de nitrógeno, potasio, calcio y magnesio hasta en 12 % (37.1 a 41.5 mg maceta-1), 8.6 % (35.3 a 38.3 mg maceta-1), 13.5 % (21.8 a 24.7 mg maceta-1) y 7.5 % (9.8 a 10.53 mg maceta-1), respectivamente, en el brote con la aplicación de sustancias húmicas, excepto en la acumulación de fósforo.

RESUMO O presente relato descreve um caso de gêmeos siameses do tipo cefalotoraconfalopago deradelfo em gatos. Uma fêmea felina foi atendida na emergência do hospital veterinário em trabalho de parto distócico. Os natimortos foram encaminhados para avaliação radiográfica e constatou-se que apresentavam um único crânio e duas colunas vertebrais completas e distintas. O exame anatomopatológico evidenciou que os gêmeos possuíam cabeça, tórax e umbigo como principais pontos de união, sendo classificados como cefalotoraconfalopago. Além disso, os gêmeos apresentaram face única e bem desenvolvida, o que permitiu classificá-los como deradelfos. Esse tipo de malformação é raro em animais domésticos, e não foi encontrado nenhum trabalho em felino com a mesma classificação do presente relato. Há necessidade de mais estudos sobre essa alteração embrionária, pois a etiologia do processo ainda não foi esclarecida.

ABSTRACT The present report describes a case of conjoined twins of the cephalo-thoraco-omphalopagus deradelphous type in cats. A feline female was transferred to our veterinary hospital as an emergency for dystocic labor. The stillborn was subjected to radiographic evaluation, and a single skull and two complete distinct vertebral columns were found. Anatomopathological examination revealed that the twins presented the head, chest, and umbilicus as the main points of union and were classified as the cephalo-thoraco-omphalopagus type. In addition, the twins had unique and well-developed faces, which allowed them to be classified as deradelphous. This malformation is rare in domestic animals, and to the best of our knowledge, this type has not been reported in felines. Further studies are warranted on this embryonic alteration, primarily because its etiology remains unknown.

This study evaluated histological changes in the digestive organs and kidneys of sheep fed sugarcane silage augmented with detoxified castor bean meal (CBM). Forty Santa Inês sheep (initial weight 20 ± 0.3 kg) were adapted to the experimental regime for 14 days, after which data were collected for 60 days. The treatments consisted of S0: sugarcane silage (SC); S5: SC augmented with 5% CBM; S10: SC augmented with 10% CBM; S15: SC augmented with 15% CBM; and S20: SC augmented with 20% CBM. After 74 days of confinement, the animals were slaughtered, and then fragments of the liver, rumen, intestine, and kidney were collected for histomorphometric analyses. The intake of dry matter and the average weight gain increased linearly with the level of CBM (P <0.05), as did the rumen absorption area, the height of the papillae and the width of the papillae. The thickness of the keratinized portion of the epithelium was not influenced, Thus, no effect was detected of the anti-nutritional factor of CBM on this organ. In the intestine, CBM increased the height of the villi and the number of goblet cells. However, S20 caused some kidney damage and decreased hepatic glycogen stock, but not sufficiently to reduce performance. Thus, augmentation of sugarcane with CBM, up to 20%, is viable in feeding sheep.

RESUMO A fim de avaliar o efeito de diferentes doses da rbST sobre a dinâmica folicular, a produção e a maturação in vitro de oócitos, 20 vacas Sindi, divididas em três grupos, receberam um dispositivo de progesterona intravaginal, estradiol e PGF2α, além de 2mL de solução salina (grupo controle), 250 (grupo rbST 250) ou 500mg de rbST (grupo rbST 500). Cinco dias depois, realizou-se a ovum pick up, e os complexos cumulus-oócitos (CCOs) recuperados foram selecionados, classificados e maturados in vitro. Os dados de contagem foram comparados pelo procedimento glht (General Linear Hypothesis Test), e os dados em porcentagem foram submetidos ao qui-quadrado, no programa estatístico R, onde as diferenças foram consideradas significativas quando P<0,05. Não houve diferença (P>0,05) entre os grupos quanto à quantidade de folículos e à taxa de maturação. Os grupos rbST 250 e rbST 500 foram significativamente superiores (P<0,05) ao grupo controle em relação ao número de folículos grandes (0,42±0,20 vs. 0). O grupo rbST 500 apresentou maior (P<0,05) porcentagem de oócitos viáveis (91,52%) do que os grupos controle (67,85%) e rbST 250 (53,33%). A rbST aumenta o número de folículos grandes, e 500mg de rbST aumentam a porcentagem de oócitos viáveis em vacas Sindi.

ABSTRACT In order to evaluate the effect of different doses of rbST on the follicular dynamics, production, and in vitro maturation of oocytes, 20 Sindhi cows were divided into three groups, receiving an intravaginal progesterone device, estradiol and PGF2α, and 2mL of solution saline (Control Group), 250 (rbST 250 Group) or 500mg rbST (rbST 500 Group). Five days later, the ovum pick up was performed, and the cumulus-oocyte (CCO) complexes recovered were selected, classified, and matured in vitro. The counting data were compared by the glht (General Linear Hypothesis Test) procedure, and the percentage data were submitted to Qui- square, in the statistical program R, where differences were considered significant when P< 0.05. There was no difference (P> 0.05) between the groups regarding follicle quantity and maturation rate. The rbST 250 and rbST 500 groups were significantly higher (P< 0.05) than the Control group in relation to the number of large follicles (0.42±0.20 versus 0). The rbST 500 group presented higher (P< 0.05) percentage of viable oocytes (91.52%) than the Control (67.85%) and rbST 250 (53.33%) groups. rbST increases the number of large follicles and 500mg rbST increases the percentage of viable oocytes in Sindhi cows.

RESUMO Infecções por Ehrlichia em bovinos são frequentes na África, mas também foram relatadas no Brasil e na América do Norte. Este artigo relata uma infecção natural por Ehrlichia sp. associado a Babesia bigemina e Anaplasma marginale em um bezerro, no município de Campo Grande, Mato Grosso do Sul, Brasil, o qual apresentava polioencefalomalácia. A evidência molecular, baseada em um fragmento do gene dsb, indica uma espécie de Ehrlichia geneticamente relacionada a Ehrlichia canis e outras espécies do gênero encontradas no carrapato Rhipicephalus (Boophilus) microplus e em um bezerro do Brasil (99 a 100% de identidade). Não foi possível associar os sinais clínicos à infecção por Ehrlichia devido a coinfecções e evidências histológicas de outra doença. No entanto, a circulação da bactéria em bovinos no Cerrado brasileiro foi confirmada, e mais atenção deve ser dada à suspeita clínica de patógenos transmitidos por carrapatos em bovinos para esclarecer o potencial patogênico de Ehrlichia sp.

ABSTRACT Ehrlichia infections in cattle are frequent in Africa but have also been reported in Brazil and North America. This paper reports natural infection by Ehrlichia sp. associated with Babesia bigemina and Anaplasma marginale in a calf in the municipality of Campo Grande, state of Mato Grosso do Sul, Brazil, presenting polioencephalomalacia. The molecular evidence, based on a fragment of the dsb gene, indicates a species of Ehrlichia genetically related to Ehrlichia canis and other species of the genus found in the tick Rhipicephalus (Boophilus) microplus and a calf from Brazil (99 to 100% identity). It was not possible to associate the clinical signs with Ehrlichia infection due to co-infections and histological evidence of another disease. However, the circulation of the bacteria in bovines in Brazilian Cerrado was confirmed and more attention should be given to clinical suspicion of tick-borne pathogens in cattle to clarify the pathogenic potential of Ehrlichia sp.

Objective: To evaluate the serum leptin levels in cannabis smokers. Methods: This was a cross-sectional population-based study of participants between the ages of 18 and 35 years. The data were collected through a self-administered questionnaire covering sociodemographic data and the use of psychoactive substances. Leptin levels were measured using a commercial ELISA kit. Results: Of the 911 participants, 6.7% were identified as cannabis smokers and had significantly lower leptin levels (p = 0.008). When stratified by gender, there was a significant decrease in leptin levels among male smokers (p = 0.039). Conclusion: Cannabis smoking was linked to leptin levels in men, suggesting that the response to biological signals may be different between men and women.

Abstract Selective non-catalytic reduction (SNCR) has become an important technique for reducing NOx emissions in industrial furnaces. The occurrence of convective recirculation can cause significant changes in SNCR performance. This paper presents the results of a study performed by Computational Fluid Dynamics (CFD) considering: (i) changes of initial NOX; (ii) injection plan and (iii) NH3/NO molar ratio. The simulations have demonstrated that the convective recirculation (resultant from different injection plans and high temperature gradients) of flue gas assumes a role of vital importance for performance analysis of existing plants and the potential NOX reduction in furnaces during the design phase.

Resumo Neste trabalho o processo simplificado de obtenção dos esforços finais utilizando o coeficiente γ z é estudado levando-se em conta a variação dos efeitos de segunda ordem ao longo da altura dos edifícios. Com este objetivo, diversos edifícios de médio porte em concreto armado são processados em primeira e segunda ordem utilizando o programa ANSYS. Inicialmente, mostra-se que o coeficiente γ z deve ser utilizado como majorador dos momentos de primeira ordem para a obtenção dos momentos finais. Assim, o estudo é conduzido considerando a relação entre os momentos obtidos pela análise em segunda e em primeira ordem, calculada para cada pavimento das estruturas. Esta relação entre os momentos é denominada de majorador dos momentos de primeira ordem, "γ", e, na situação ideal, deve coincidir com o valor de γ z . Entretanto, observa-se que a razão γ /γ z varia ao longo da altura dos edifícios. Além disso, mostra-se, por meio de uma análise estatística, que a relação γ /γ z apresenta-se geralmente inferior a 1,05 e varia significativamente de acordo com o edifício considerado e a presença ou não de simetria na estrutura.

Abstract In this paper the simplified method to evaluate final efforts using γ z coefficient is studied considering the variation of the second order effects with the height of the buildings. With this purpose, several reinforced concrete buildings of medium height are analyzed in first and second order using ANSYS software. Initially, it was checked that the (z coefficient should be used as magnifier of first order moments to evaluate final second order moments. Therefore, the study is developed considering the relation (final second order moments/ first order moments), calculated for each story of the structures. This moments relation is called magnifier of first order moments, "γ", and, in the ideal situation, it must coincide with the γ z value. However, it is observed that the reason γ /γ z varies with the height of the buildings. Furthermore, using an statistical analysis, it was checked that γ /γ z relation is generally lower than 1.05 and varies significantly in accordance with the considered building and with the presence or not of symmetry in the structure.

RESUMO Objetivou-se com esta pesquisa comparar diferentes modelos de regressão aleatória e determinar o mais adequado para descrever mudanças nos parâmetros de avaliação do crescimento de bovinos da raça Tabapuã criados no Nordeste brasileiro. O modelo de regressão aleatória M4532-5 foi definido como sendo o de melhor ajuste para descrição das estimativas de variância e herdabilidades das características de crescimento dos animais avaliados. As estimativas de variância genética aditiva direta aumentaram em função da idade, já as de variância genética aditiva materna mostraram crescimento do nascimento até próximo aos 420 dias. As correlações genéticas entre as quatro primeiras características foram positivas e de magnitudes moderada a alta. A maior correlação genética foi observada entre o peso ao nascer e aos 240 dias (0,82). A correlação fenotípica entre peso ao nascimento e demais características foi baixa. O modelo de regressão aleatória M4532-5 com 39 parâmetros apresentou-se como aquele de melhor ajuste para descrever a curva de crescimento dos animais avaliados. Resposta à seleção para obtenção de animais mais pesados será eficiente quando realizada em idades posteriores ao desmame. Ao se avaliar a curva de crescimento de bovinos por meio da interpretação dos parâmetros genéticos estimados, é possível selecionar animais com maior precocidade de abate.

ABSTRACT The objective of this study is to compare random-regression models used to describe changes in evaluation parameters for growth in Tabapuã bovine raised in the Northeast of Brazilian. The M4532-5 random-regression model was found to be best for estimating the variation and heritability of growth characteristics in the animals evaluated. Estimates of direct additive genetic variance increased with age, while the maternal additive genetic variance demonstrated growth from birth to up to nearly 420 days of age. The genetic correlations between the first four characteristics were positive with moderate to large ranges. The greatest genetic correlation was observed between birth weight and at 240 days of age (0.82). The phenotypic correlation between birth weight and other characteristics was low. The M4532-5 random-regression model with 39 parameters was found to be best for describing the growth curve of the animals evaluated providing improved selection for heavier animals when performed after weaning. The interpretation of genetic parameters to predict the growth curve of cattle may allow the selection of animals to accelerate slaughter procedures.

RESUMO Os herbicidas aminocyclopyrachlor e indaziflam encontram-se em fase de desenvolvimento no Brasil. Informações a respeito do comportamento deles em condições edafoclimáticas brasileiras são escassas. O presente trabalho foi desenvolvido com o objetivo de avaliar a persistência da atividade biológica e o potencial de lixiviação do aminocyclopyrachlor e indaziflam em solos de texturas contrastantes, por meio de bioensaios. Para avaliação da persistência foram realizados quatro experimentos, em que foram estudados solos com texturas distintas em diferentes períodos de tempo entre a aplicação dos herbicidas e a semeadura do bioindicador (beterraba e soja). Para determinação do potencial de lixiviação, simularam-se três lâminas de precipitação (0, 30 e 60 mm), cada uma constituindo um experimento isolado, em solos com diferentes texturas e cinco faixas de profundidades nas colunas. O bioindicador utilizado para os ensaios de lixiviação foi a beterraba. A persistência da atividade biológica do aminocyclopyrachlor e indaziflam foi superior a 150 dias. No solo de textura muito argilosa, verificou-se menor persistência do aminocyclopyrachlor do que no de textura franco-argiloarenosa. Para o indaziflam, não se observou diferença na persistência entre os dois solos estudados. Com relação ao potencial de lixiviação, observou-se que as precipitações possuem capacidade de interferir na lixiviação dos herbicidas aminocyclopyrachlor e indaziflam. O aminocyclopyrachlor possui maior potencial de lixiviação do que o indaziflam; o primeiro tem sua mobilidade aumentada quando aplicado em solo de textura franco-argiloarenosa, em relação ao solo de textura muito argilosa. Quanto ao indaziflam, não foram notadas diferenças marcantes entre os solos com diferentes texturas.

ABSTRACT Herbicides aminocyclopyrachlor and indaziflam are under development in Brazil. Information about the behaviors in Brazilian soils and climate is scarce. Thus, the present work has aimed to evaluate the persistence of biological activity and leaching potential of aminocyclopyrachlor and indaziflam in contrasting textured soils by means of bioassays. For the evaluation of persistence, four experiments were performed, in which soils with different textures were studied in different time periods between herbicide application and bioindicator sowing (beet and soybeans). To determine leaching potential, three blades of rainfall (0, 30 and 60 mm) were simulated, each constituting a single experiment in soils with different textures and five bands of depth in the columns. The bioindicator used for the leaching tests was beet. The persistence of biological activity of aminocyclopyrachlor and indaziflam was greater than 150 days. In clayey soil there was less persistent aminocyclopyrachlor than in the loam texture. For indaziflam there was no difference in persistence between the two soils. Regarding the leaching potential, it was observed that the precipitations have the capacity to interfere with the leaching of herbicides aminocyclopyrachlor and indaziflam. Aminocyclopyrachlor has greater potential for leaching than indaziflam. The first one has its mobility increased when applied to soil of loam texture in relation to very clayey soil. As for indaziflam, marked differences between soils with different textures have not been noticed.

A produção in vitro de embriões suínos tem alcançado resultados insatisfatórios: ovócitos maturados in vivo produzem uma porcentagem maior de embriões em relação aos maturados in vitro. O sucesso da maturação in vitro está diretamente relacionado com a competência ovocitária. Somente ovócitos competentes são capazes de serem fecundados e terem desenvolvimento embrionário normal. A competência ovocitária pode ser avaliada por vários parâmetros. Recentemente têm sido utilizados como parâmetro os estudos da expressão de genes associados com a competência. O presente trabalho teve por objetivo avaliar diferenças na expressão dos genes BMP15, RYBP, MATER e ZAR1 em ovócitos imaturos de diferentes classes morfológicas, sendo elas: 1, 2, 3 e 4, com a finalidade de proporcionar importantes marcadores moleculares relacionados com a capacidade ovocitária. O RNA total dos ovócitos foi extraído e utilizado como molde para a síntese da primeira fita de cDNA. Os resultados da expressão gênica foram analisados utilizando-se modelo misto, considerando os dados de expressão gênica variável dependente e as classes ovocitárias variáveis independentes. Os genes BMP15, ZAR1 e RYBP apresentaram expressão semelhante nas classes ovocitárias 1, 2 e 3; somente a categoria 4 diferiu na expressão desses genes (P<0,05). O gene MATER foi expresso de forma semelhante em todas as classes ovocitárias estudadas (P>0,05). A técnica de RT-qPCR foi eficiente para detecção desses transcritos em ovócitos de diferentes classes. No entanto, para melhor entendimento do envolvimento desses transcritos na aquisição da competência ovocitária, são necessários mais estudos avaliando ovócitos de diferentes classes morfológicas, em diferentes fases de desenvolvimento, e implicação de outros genes envolvidos com a competência ovocitária.

The in vitro production of pig embryos has achieved unsatisfactory results; in vivo matured oocytes produce a higher percentage of embryos compared to in vitro maturation. The success of in vitro maturation is directly related to oocyte competence. Only competent oocytes are capable of being fertilized and have normal embryonic development. The oocyte competence can be assessed using several parameters. Recently these parameters have been used for gene expression studies associated with competence. This work aimed to evaluate differences in gene expression BMP15, RYBP, MATER, ZAR1 as endogenous control and the constitutive gene GAPDH in immature oocytes of different morphological classes which are: 1, 2, 3 and 4, in order to provide significant molecular markers linked to the ability of development. Oocytes Total RNA was extracted and used as a template for synthesis of the first cDNA strand. The results of gene expression were analyzed using a mixed model, considering the dependent gene expression data and independent ovocitary variable classes. The genes BMP15, RYBP ZAR1 and showed similar ovocitary expression in classes 1, 2 and 3 differ only in category 4 in their expression (P<0.05). The MATER gene was similarly expressed in all ovocitary classes studied (P>0.05). The RTQ-PCR technique was effective for detection of these transcripts in oocytes from different classes. However, for better understanding of the involvement of these transcripts in the acquisition of oocyte competence more studies are needed to evaluate different morphological classes of oocytes at different stages of development and the implication of other genes involved in oocyte competence.