RESUMO A deficiência de vitamina A é frequente nos países em desenvolvimento. Neste contexto, a batata-doce pode ser uma aliada na reversão desse quadro, uma vez que apresenta baixo custo de aquisição e elevada disponibilidade de mercado. Além disso, alguns genótipos apresentam polpa de coloração laranja, rica em betacaroteno, que é o precursor da vitamina A. Assim, objetivou-se com a presente pesquisa selecionar genótipos de batata-doce de polpa laranja por meio do emprego de dois índices de seleção (Smith e Hazel e Mulamba & Mock). Para tanto, foram avaliadas sete famílias de meio irmãos com raízes de polpa laranja (141 genótipos experimentais e a cultivar comercial Beauregard), verificando-se os parâmetros produtivos, aparência externa das raízes, a intensidade de cor da polpa e suscetibilidade à Euscepes postfasciatus. Dessa forma, os genótipos UZBD-C-14, UZBD-U1-25, UZBD-F-15, UZBD-C-30, UZBD-K-32, UZBD-U1-10, UZBD-L2-14 e UZBD-L5-67 apresentaram-se mais promissores, demonstrando maior equilíbrio para os caracteres avaliados. Ademais, esses genótipos estão aptos a novos estudos, com propósito de confirmar o desempenho produtivo e de qualidade de raízes, além dos parâmetros bioquímicos que comprovem a herança do caráter que regula a biofortificação mediada pelos carotenos.

ABSTRACT Vitamin A deficiency is common in developing countries. Sweet potato can be an ally in reversing this situation since it has a low acquisition cost and high market availability. In addition, some genotypes have orange-flesh roots, rich in beta-carotene, which is the precursor of vitamin A. Thus, the objective of this research was to select orange-fleshed sweet potato genotypes using two selection indices (Smith and Hazel and Mulamba & Mock). For this purpose, seven half-sibling families with orange flesh roots (141 experimental genotypes and the commercial cultivar Beauregard) were evaluated, assessing yield-related traits, external appearance of roots, pulp color intensity, and susceptibility to Euscepes postfasciatus. The genotypes UZBD-C-14, UZBD-U1-25, UZBD-F-15, UZBD-C-30, UZBD-K-32, UZBD-U1-10, UZBD-L2-14, and UZBD-L5-67 were the most promising, showing greater balance for the evaluated characters. Furthermore, these genotypes are suitable for new studies to confirm their productive performance and root quality and evaluate the biochemical parameters that prove the inheritance of the character regulating biofortification mediated by carotenes.

ABSTRACT The objective of this study was to evaluate the cholecalciferol and 1,25-dihydroxycholecalciferol glycoside supplementation in piglet diet on organ biometry and physicochemical composition, pH of the digestive tract contents, and bone traits. A total of 128 entire male piglets (21 days-old, 6.82±0.38 kg body weight) were distributed in a completely randomized block design with eight replications and four animals per experimental unit. The treatments were composed of a diet 100/0 = 100% of the vitamin D supplemented with cholecalciferol; 50/50 = 50% supplemented with cholecalciferol + 0.25 μg of 1,25(OH)2D3 glycoside; 25/75 = 25% supplemented with cholecalciferol + 0.375 μg of 1,25(OH)2D3 glycoside; and 0/100 = 0.50 μg of 1,25(OH)2D3 glycoside. The results indicated that piglets fed 100/0 showed an increase in spleen weight compared with those fed 0/100 and greater heart dry matter than piglets fed the 50/50 diet. Phosphorus concentration in the spleen was higher in piglets that received 25/75 when compared with those that received the 100/0 and 0/100 diets. Piglets that received the 0/100 diet showed higher pH of the stomach contents, but showed reduced pH of the contents of the jejunum and ileum. The width of the epiphysis and diaphysis was greater when piglets consumed the 50/50 treatment; however, a lower epiphysis height was observed. The use of 1,25(OH)2D3 glycoside alone in piglet diets does not negatively influence the pH of the gastrointestinal tract and organ physicochemical composition. In addition, the use of diets with 1,25(OH)2D3 glycoside as a replacement for cholecalciferol reduces spleen weight and promotes improvements in bone development.

Abstract Introduction Cortical auditory evoked potentials (CAEPs) are bioelectric responses that occur from acoustic stimulations, and they assess the functionality of the central auditory system. Objective The objective of the present study was to analyze the effect of musical stimulation on CAEPs. Methods The sample consisted of 42 healthy female subjects, aged between 18 and 24 years, divided into two groups - G1: without musical stimulation prior to the CAEP examination; and G2: with stimulation prior to the examination. In both groups, as a pre-collection procedure, the complete basic audiological evaluation was performed. For the musical stimulation performed in G2, we used an MP4 player programmed to play Pachelbel’s “Canon in D Major” for five minutes prior to the CAEP examination. To analyze the effect on the groups, the ear side and the ide-group interaction , a mixed analysis of variance (ANOVA) of repeated measures was performed. Box M test and Mauchly sphericity test were also performed. Results Test differences were considered statistically significant when the p-value was < 0.05 (5%). Thus, it was possible to observe that there was a statistically significant difference of the P2 component characterized by the decrease in the amplitude of response in the left ear in G2 when comparing the responses of CAEP with and without prior musical stimulation. Conclusion The result of the present study enabled us to conclude that there was a change in the response of CAEPs with musical stimulation.

RESUMO: A leptospirose é uma doença infecciosa causada por bactérias do gênero Leptospira, que afeta animais domésticos, selvagens e também humanos. De outubro a novembro de 2014, numa propriedade rural localizada em Glorinha, RS, em que bovinos eram mantidos em resteva de arroz, 13 bezerros manifestaram hemoglobinúria e apatia, nove dos quais morreram em menos de 24 horas após o início dos sinais clínicos. Foram necropsiados quatro bezerros (A, B, C e D). Fragmentos de tecido foram fixados em formalina a 10%. Amostras de rim, fígado e pulmão dos Bezerros B, C e D foram enviadas para análise de PCR para RNA ribossômico 16S e a proteína Lip 32 de Leptospira. No exame macroscópico foram observados mucosas e tecido subcutâneo amarelados, fígado alaranjado, pulmões com múltiplas petéquias, predominantemente nos lóbulos craniais. A cavidade torácica do Bezerro A estava repleta de um líquido vermelho-escuro. À avaliação microscópica foi observada hemorragia acentuada nos pulmões; no fígado havia necrose e vacuolização hepatocelular centrolobular difusa moderada, além de infiltrado linfocítico periportal discreto. Nos rins observou-se nefrite intersticial linfoplasmocítica discreta multifocal. A análise por PCR teve resultado positivo para os Bezerros B e D. O diagnóstico de leptospirose nos bezerros foi baseado nos achados epidemiológicos, clínicos e patológicos, associados ao resultado positivo na PCR. Este estudo demonstra a importância da investigação da doença quando animais jovens são criados em áreas inundadas e têm manifestações clínicas de doença septicêmica aguda.

ABSTRACT: Leptospirosis is an infectious disease caused by bacteria of the genus Leptospira, which affect domestic and wild animals, and also humans. From October to November 2014, in a rural property located in Glorinha, RS, where cattle were kept in the rice stubble, thirteen calves presented hemoglobinuria and apathy, nine of which died within less than 24 hours after the onset of clinical signs. Four calves were necropsied (A, B, C and D). Tissue samples were collected in 10% formalin. Samples of kidney, liver and lung from calves B, C and D were sent for PCR analysis for 16S ribosomal RNA and the protein Lip 32 genes of Leptospira. At macroscopic examination jaundiced mucosae and subcutaneous tissue, orange liver, and lungs with multiple petechiae, predominantly in cranial lobes, were observed. The thoracic cavity of calf A was filled with a reddish fluid. At microscopic examination, severe hemorrhage was observed in the lungs; in the liver there was moderate diffuse centrilobular hepatocellular necrosis and vacuolization, in addition to discrete periportal lymphocytic infiltrate. Discrete multifocal lymphoplasmocytic interstitial nephritis was observed in the kidneys. PCR analyzis resulted positive for calves B and D. The diagnosis of leptospirosis in the calves was based on epidemiological, clinical and pathological findings associated with positive PCR analysis. This study demonstrates the importance of investigation of the disease when young bovids are raised in flooded areas and have clinical signs of an acute septicemic disease.

RESUMO CONTEXTO A vitamina D é conhecida por possuir propriedades imunomoduladoras, anti-inflamatórias e antifibróticas, relevantes na patogênese e tratamento de muitas causas de doença hepática crônica. OBJETIVO Este estudo tem como objetivo avaliar a associação entre os níveis séricos de vitamina D e os achados histopatológicos em pacientes com infecção crônica do vírus da hepatite C. MÉTODOS Estudo transversal, composto por pacientes com hepatite C crônica. Todos os pacientes foram submetidos à dosagem de vitamina D 25 e análise de dados antropométricos. A biópsia hepática foi realizada em um período máximo de 36 meses antes da inclusão no estudo. RESULTADOS Dos 74 pacientes incluídos no estudo, 45 (60,8%) eram mulheres, média de idade de 57,03±9,24 anos e 63 (85,1%) eram brancos. Não foi observada associação entre os níveis séricos de vitamina D e atividade inflamatória (P=0,699), nem com o grau de fibrose hepática (P=0,269). CONCLUSÃO No presente estudo, não foi observada associação entre a vitamina D e a atividade inflamatória, bem como com o grau de fibrose hepática, em pacientes com hepatite C crônica.

ABSTRACT BACKGROUND Vitamin D is known for its immunomodulatory, anti-inflammatory and antifibrotic properties, which are quite relevant in the pathogenesis and treatment of many causes of chronic liver disease. OBJECTIVE This study aimed to evaluate the association between serum vitamin D levels and the histopathological findings in patients with chronic hepatitis C virus infection. METHODS Cross-sectional study composed of patients with chronic hepatitis C. All patients underwent vitamin D 25 dosage and anthropometric data analysis. Liver biopsy was performed in a maximum 36-month period before inclusion in the study. RESULTS Of the 74 patients included in the study, 45 (60.8%) were women, mean age was 57.03±9.24 years, and 63 (85.1%) were white. No association was observed between the serum levels of vitamin D and inflammatory activity (P=0.699) nor with the degree of liver fibrosis (P=0.269). CONCLUSION In this study, no association was observed between vitamin D and inflammatory activity, as well as the degree of liver fibrosis, in patients with chronic hepatitis C.

ABSTRACT An experiment was conducted to determine the required levels of supplementary vitamin D and calcium in meat-type quail (Coturnix coturnix sp) between 15 and 35 days old. The experiment was a 4 × 4 factorial design with four levels of calcium: 0.42, 0.58, 0.74, and 0.90% and four levels of vitamin D: 1,000; 2,000; 3,000; and 4,000 IU. Body weight and body weight gain increased linearly and feed conversion showed some linear improvement due to increased levels of Ca and vitamin D. The increased vitamin D levels resulted in a linear increase in feed intake. Calcium and vitamin D requirements in meat-type quail between 15 and 35 days of age is greater or equal to 0.90% Ca and greater or equal to 4,000 IU of vitamin D, probably because the experiment was conducted during the pre-laying phase.

Resumo: Poucos estudos compararam a soroprevalência de aglutininas antileptospira com a demonstração de excreção urinária de leptospiras ou evidência de infecção ativa em primatas não humanos. A população estudada consistiu em 58 animais, sendo 42 primatas do Parque Zoobotânico Getúlio Vargas, Bahia, Brasil. O estudo avaliou ainda 16 primatas (Cebus sp.) resgatados do tráfico ilegal e abrigados no Centro de Triagem de Animais Silvestres Chico Mendes, Salvador, Bahia, Brasil. A soroprevalência de anticorpos antileptospira foi baixa (2%) nos animais do Zoológico. Uma taxa mais elevada (31%) foi observada nos animais resgatados do tráfico ilegal. Mesmo que todas as amostras de sangue e urina tenham sido negativas para DNA de leptospiras, a alta frequência de evidência de exposição nos animais de origem selvagem indicam o risco potencial da adoção de primatas como animais de estimação.

Abstract: Few studies have compared the seroprevalence of antileptospiral agglutinins with the demonstration of urinary shedding of leptospires or evidence of active infection in the bloodstreams of non-human primates. The study population consists of 58 animals, including d 42 monkeys from the Zoological Park of Salvador (Parque Zoobotânico Getúlio Vargas), Bahia, Brazil. The study also evaluated 16 primates (Cebus sp.) rescued from illegal trade that were housed in the Wildlife Rehabilitation Center of Salvador (CETAS), Bahia, Brazil. The seroprevalence of antileptospiral antibodies was low (2%) in the animals from the Zoo. A higher rate (31%) was observed among the animals that were rescued from illegal trade in the state of Bahia. Even if all the blood and urine samples were negative for leptospiral DNA fragments, the high frequency of serological evidence of exposure suggests a potential risk of leptospirosis transmission when keeping these animals as pets.

Um rapaz de 19 anos, previamente hígido, procurou o hospital com queixas de anorexia, náuseas e vômitos. Exames laboratoriais revelaram hipercalcemia (valor máximo do cálcio de 14,8 mg/dL) e lesão renal aguda (valor máximo da creatinina de 2,88 mg/dL). O paciente admitiu utilizar uma formulação parenteral de vitaminas A, D e E de uso exclusivo veterinário, contendo 20.000.000 UI de vitamina A; 5.000.000 UI de vitamina D3 e 6.800 UI de vitamina E, por ampola de 100 mL. Ele refere ter usado cerca de 300 mL do produto no último ano. O jovem não estava interessado na quantidade maciça de vitaminas contida no produto, mas apenas no efeito local do veículo oleoso; o edema provocado pela injeção simulava um aumento de massa muscular. O produto, no entanto, foi absorvido e causou hipervitaminose. O nível sérico de 25(OH) vitamina D estava claramente elevado em 150 ng/mL (referência de 30 a 60 ng/mL), mas não tanto quanto em outros casos publicados de intoxicação por vitamina D. A maioria dos casos de hipercalcemia por hipervitaminose D se associa a níveis de 25 (OH)D bem maiores do que 200 ng/mL. O PTH estava indetectável, e outras causas de hipercalcemia foram excluídas. Deste modo, conclui-se que a gravidade da hipercalcemia encontrada neste caso foi resultado do efeito sinérgico da intoxicação pelas vitaminas A e D. O paciente foi tratado com soro fisiológico, furosemida e ácido zolendrônico e evoluiu com normalização rápida dos níveis séricos de cálcio e da função renal.

A previously healthy 19 year-old male presented to the hospital with anorexia, nausea, and vomiting. Laboratory studies were significant for hypercalcemia (peak calcium value of 14.8 mg/dL) and acute kidney injury (peak serum creatinine of 2.88 mg/dL). He admitted to using a parenteral formulation of vitamins A, D and E restricted for veterinary use containing 20,000,000 IU of vitamin A; 5,000,000 IU of vitamin D3; and 6,800 IU of vitamin E per 100 mL vial. The patient stated to have used close to 300 mL of the product over the preceding year. Interestingly, the young man was not interested in the massive amounts of vitamins that the product contained; he was only after the local effects of the oily vehicle. The swelling produced by the injection resulted in a silicone-like effect, which gave the impression of bigger muscles. Nevertheless, the product was absorbed and caused hypervitaminosis. The serum level of 25(OH) vitamin D was clearly elevated at 150 ng/mL (reference range from 30 to 60 ng/mL), but in most published cases of vitamin D toxicity, serum levels have been well above 200 ng/mL. His PTH level was undetectable and other potential causes of hypercalcemia were excluded. Therefore, we posit that the severity of the hypercalcemia observed in this case was the result of a synergistic effect of vitamins A and D. The patient was treated with normal saline, furosemide and zolendronic acid, with rapid normalization of calcium levels and renal function.

O excesso de peso está associado a inúmeros fatores, dentre eles, o nível socioeconômico das famílias. Assim, este trabalho objetivou avaliar a relação entre os níveis de sobrepeso e obesidade e a classe econômica de crianças de 6,0 a 7,9 anos, do município de Maringá/PR. O Índice de Massa Corporal (IMC) foi utilizado para diagnóstico do estado nutricional. Para a classificação econômica familiar, foi empregada proposta da Associação Brasileira de Empresas de Pesquisa (ABEP), sendo que as classes originais foram reagrupadas em níveis econômicos alto (classes A1, A2, B1), médio (classes B2, C1 e C2) e baixo (classes D e E). O tratamento estatístico foi realizado utilizando pacote computadorizado SPSS 13.0 e testes não-paramétricos e Odds Ratio (OR) com intervalo de confiança (IC) de 95%. Foram avaliadas 1634 crianças de 24 escolas públicas e privadas, com idade média de 7,1±0,51 anos, sendo 50,8% do sexo feminino. Não foram observadas diferenças significativas entre os gêneros, para os valores de IMC, das crianças com sobrepeso e obesidade. A prevalência de excesso de peso (sobrepeso+obesidade) foi de 22,3% entre os meninos e de 21,9% entre as meninas. Não houve associação estatística entre o excesso de peso e o gênero. Por outro lado, crianças do nível econômico A apresentaram 28% mais chance de apresentar excesso de peso que aquelas do nível B. Concluindo, neste estudo, ambos os gêneros foram igualmente afetados pelo excesso de peso, com maior prevalência entre os meninos, e as crianças pertencentes a famílias de níveis econômicos inferiores apresentaram menor chance de apresentarem excesso de peso.

Excess weight is associated with various factors, including socioeconomic level. The objective of this study was to evaluate the relationship between overweight and obesity and socioeconomic level in children aged 6.0-7.9 years from Maringá, Paraná. Body mass index (BMI) was used for the assessment of nutritional status. Family socioeconomic status was classified based on the proposal of the Brazilian Association of Research Companies (ABEP), and the originally proposed classes were regrouped into high (A1, A2, and B1), medium (B2, C1, and C2) and low (D and E) socioeconomic level. The data were analyzed statistically with the SPSS 13.0 program using nonparametric tests and odds ratios with a 95% confidence interval (CI). A total of 1,634 children from 24 public and private schools (mean age: 7.1 ± 0.5 years, 50.8% girls) were studied. No significant difference in BMI was observed between boys and girls with overweight and obesity. The prevalence of excess weight (overweight + obesity) was 22.3% and 21.9% among boys and girls, respectively. There was no significant association between excess weight and gender. However, children of high socioeconomic level had a 28% higher chance of excess weight than those of low level. In conclusion, in this study both boys and girls were equally affected by excess weight, with a higher prevalence among boys. Children from families of lower socioeconomic levels presented a lower chance of excess weight.

Observou-se hipertrofia das células do epitélio intestinal de Aedes aegypti (L.), Anopheles albitarsis (Lynch-Arribálzaga) e Culex quinquefasciatus (Say) devido ao aumento da atividade secretora intracelular como primeira reação às toxinas das linhagens 2362 e S1116 de Bacillus sphaericus (Neide). Após a hipertrofia epitelial, formaram-se vesículas na porção apical das células, as quais eram compostas de fragmentos de membrana plasmática contendo material de natureza desconhecida em seu interior, caracterizando um tipo de secreção apócrina. A via de contaminação das larvas dos mosquitos por essas bactérias é pelo intestino, através da alimentação. Dependendo da espécie de Culicidade e da linhagem bacteriana utilizada, a hipertrofia do epitélio ocorreu entre 5 a 15 min após a exposição das larvas ao meio contaminado. O segundo aspecto observado no processo de contaminação foi o aumento da atividade de secreção apócrina. As vesículas basófilas que se desprendiam das células permaneciam entre a membrana peritrófica e o lúmen intestinal. Observou-se atividade secretora tanto no grupo controle como no experimental, porém muito mais intensa no grupo experimental. Os Culicidae estudados apresentaram diferenças marcantes nas respostas às toxinas das bactérias utilizadas, sendo C. quinquefasciatus a espécie mais suscetível. As diferenças de reações das células em relação às toxinas produzidas pelas duas linhagens bacterianas são discutidas.

Cell hypertrophy was the first reaction of the gut epithelial cells of Aedes aegypti (L.), Anopheles albitarsis (Lynch-Arribálzaga) and Culex quinquefasciatus (Say) to the toxins of strains 2362 and S1116 of Bacillus sphaericus, as cells had an increase of intracellular secretory activity. Soon after the cell hypertrophy developed, vesicles were formed at the cell apical portion, which detached with the plasma membrane, characterizing a type of apocrine secretion like. The first pathway of contamination of the mosquito larvae by these bacteria is through the gut, by feeding. Depending on the species of Culicidae and on the bacterial strain used, the hypertrophy of the gut epithelium occurred between 5 and 15 min after exposure to the contaminated environment. The second aspect observed after hypertrophy was the increase in apocrine secretion. The basophilic vesicles that detached from the cells remained between the peritrophic membrane and the gut lumen, such vesicles were filled with material of unknown nature. The gut posterior region showed secretory activity in both control and treated larvae, being much more intense in bacteria-exposed larvae. There were remarkable differences in the epithelial cell reaction according to the toxins of the two bacterial strains, but C.quinquefasciatus was the most susceptible. Differences in the gut cell reactions to the toxins produced by the two bacterial strains are discussed.