RESUMO Objetivo Avaliar a influência do tratamento térmico do silano na resistência de união entre dois cimentos resinosos e uma cerâmica feldspática CAD/CAM. Métodos Quarenta blocos cerâmicos foram duplicados em resina micro-híbrida. Todas as cerâmicas foram condicionadas com ácido fluorídrico 10% por 20 s e receberam aplicação do silano. Os blocos foram randomizados em oito grupos (n = 5): P-Panavia F; PAg- Panavia F + Envelhecimento; HP-Tratamento térmico + Panavia F;HPAg-Tratamento térmico + Panavia F + Envelhecimento; R-RelyX ARC; RAg-RelyX ARC; HR-Tratamento térmico + RelyX ARC; HRAg - Tratamento térmico + RelyX ARC + Envelhecimento. O tratamento térmico foi realizado em forno a 100 °C durante2 min. Todos os blocos foram cimentados e cortados para obter amostras para microtração. Foram feitos 10000 ciclos térmicos. As amostras foram submetidas a teste de micro-tração. Resultados O teste ANOVA revelou uma interação significativa entre cimento/tratamento térmico (p=0,001) e tratamento térmico/ciclo térmico (p=0,001). Conclusão Dessa maneira, o tratamentotérmico do silano a 100ºC por 2 minutos foi capaz de aumentar a resistência de união entre o cimento resinoso e uma cerâmica feldspática mesmo após o envelhecimento.

ABSTRACT Objective Evaluate the influence of silane heat treatment in bond durability between two resin cements and CAD/CAM feldspathic ceramic. Methods 40 feldspathic ceramic blocks were obtained, and were duplicated in microhybrid composite. All ceramic blocks were etched with 10% hydrofluoric acid for 20 s and received application of silane agent. The blocks were randomizedinto eight groups (n=5) according to the surface treatments and luting agents: P- Panavia F; PAg- Panavia F + Aging; HP- Heat Treatment + Panavia F; HPAg- Heat Treatment + Panavia F + Aging; R- RelyX ARC; Rag- RelyX ARC + Aging; HR- Heat Treatment + RelyX ARC; HRAg- Heat Treatment + RelyX ARC + Aging. The heat treatment was performed in furnace at 100 °C for 2 min. All blocks were cemented and cut to obtain samples with adhesive area of 1 mm2. Aging was performed with 10000 thermal cycles. The samples were subjected to microtensile bond strength test. Results The three-way ANOVA test revealed a significant interaction between cement versus heat treatment (p=0.001) and heat treatment versus thermal cycling (p=0.001) indicating that there was a change in bond strength due to surface treatment and aging. Conclusion Therefore, the heat treatment of the silane at 100 ºC for 2 minutes obtained higher values of bond strength between the resin cement and a feldspathic ceramicafter aging.

The aim of this study is to evaluate a novel oxidative stress marker (thiol-disulphide homeostasis) in paediatric sepsis and to determine their effects on the prognosis of sepsis. Patients diagnosed with sepsis (n= 38) and healthy controls (n= 40) were incorporated in the study. Total thiol, native thiol, disulphide, disulphide/total thiol, disulphide/native thiol, and native thiol /total thiol levels were measured in the sepsis and control groups. Additionally, the parameters were compared between survivors and non-survivors in the sepsis group. The levels of hemoglobin, white blood cell, platelet, lactate, and C-reactive protein were measured in patients with sepsis at diagnosis. The paediatric risk of mortality and paediatric logistic organ dysfunction scores of the patients were used to estimate the disease severity. The plasma thiol levels of the patients with sepsis were significantly lower than the control group (p < 0.001). This study showed that thiol/disulphide homeostasis is abnormal in children with sepsis in Paediatric Intensive Care Unit.

Objetivo. Evaluar un novedoso marcador del estrés oxidativo (la homeostasis de tiol /disulfuro) en la sepsis pediátrica y determinar sus efectos sobre el pronóstico de esta afección. Métodos. En el estudio, se incluyeron pacientes con diagnóstico de sepsis y controles sanos. Se midieron las concentraciones de tiol total, tiol nativo, disulfuro, disulfuro /tiol total, disulfuro /tiol nativo y tiol nativo/tiol total en los grupos con sepsis y de referencia. Se compararon los parámetros entre los supervivientes y los no supervivientes del grupo con sepsis. Se midieron las concentraciones de hemoglobina, leucocitos, trombocitos, lactato y proteína C-reactiva en los pacientes con sepsis al momento del diagnóstico. Se utilizaron el puntaje de riesgo de mortalidad pediátrico (Pediatric Risk of Mortality, PRISM) y el puntaje de disfunción orgánica (Pediatric Logistic Organ Dysfunction, PELOD) para estimar la gravedad de la enfermedad. Resultados. En el grupo con sepsis se incluyó a 38 pacientes y en el de referencia, a 40 niños sanos. Las concentraciones plasmáticas de tiol en los pacientes con sepsis fueron significativamente inferiores que las del grupo de referencia (p < 0,001). Conclusión. La homeostasis de tiol/disulfuro fue anormal en los niños con sepsis en la unidad de cuidados intensivos pediátricos.

Abstract The objective of the present study was to evaluate the effect of different surface treatments and polymerization protocols on the bond strength of brackets to enamel, and the degree of conversion of the bonding agents. 120 bovine crowns were embedded in acrylic resin blocks and sanded. Next, the blocks were randomly assigned into 12 groups. Metal brackets were bonded to enamel according to the “surface treatment” factor (A: Phosphoric Acid; ATxt: Phosphoric Acid + Transbond XT Primer®; Tse: Transbond Plus Self Etching Primer®; and SBU: Scotchbond Universal®) and “polymerization” factor (R20: Radii-Cal®/20 seconds; V20: Valo Cordless®/20 seconds; and V3: Valo Cordless®/3 seconds). All samples were stored for 6 months (water, 37ºC) and then subjected to a shear bond strength test (SBS). Bond failures were classified according to the Adhesive Remnant Index (ARI) and analyzed with the Kruskal-Wallis and Mann-Whitney tests (5%). Using the same factors, 120 resin discs were made to assess the degree of conversion (DC) of the monomer. Data from the SBS (MPa) and DC (%) were analyzed by analysis of variance (2 factors) and Tukey’s test (5%). For the SBS, the factors “polymerization” (R20 = 8.1B; V20 = 13.2A; V3 = 5.2C, p = 0.0001) and “surface treatment” (A = 3.1C; ATxt = 13.6A; Tse = 12.3A; SBU = 6.3B, p = 0.0001) were statistically significant among groups. The highest adhesion value were found for the ATxt/V20 group (22.2A) and the lowest value for the A/R20 group (1.2E). Regarding ARI, score 2 was the most prevalent in groups A, ATxt, V20 and V3, while score 4 was the most prevalent in the Tse, SBU and R20 groups, with no significant difference between them (p = 1.0). Regarding DC, the factors “polymerization” (R20 = 66.6A; V20 = 58.4B; V3 = 45.1C, p = 0.0001) and “surface treatment” (A = 52B, ATxt = 59.7A, Tse = 51.4B, SBU = 63.8A, p = 0.0001) were statistically significant. Tse was more sensitive to the variations in polymerization protocols than the other surface treatments. Treatment A did not present suitable bond strength or degree of conversion.

ABSTRACT PURPOSE: To investigate the potential protective effects of enoxaparin against the adverse events of carbon dioxide (CO2) pneumoperitoneum. METHODS: Thirty four rats were divided into three groups: Group 1 (sham) underwent insertion of Veress needle into the abdomen and 90 min of anesthesia with no gas insufflation. The animals in control and enoxaparin groups were subjected to 90 min of 14 mmHg CO2 pneumoperitoneum. Enoxaparin (100 u/kg) was administered subcutaneously to the rats in enoxaparin group one hour before the operation. After 90 min of pneumoperitoneum, the rats were allowed for reperfusion through 60 min. Blood and liver samples were obtained for biochemical and histopathological examination. RESULTS: Treatment with enoxaparin decreased the histopathological abnormalities when compared with the control group. The highest levels of oxidative stress parameters were found in control group. The use of enoxaparin decreased the levels of all oxidative stress parameters, but the difference between the control and enoxaparin groups was not statistically significant. CONCLUSION: Enoxaparin ameliorated the harmful effects of high pressure CO2 pneumoperitoneum on the liver.