RESUMO O objetivo deste trabalho foi avaliar a interação genótipos x safras de diferentes caracteres, a correlação genética entre caracteres agronómicos na seleção precoce sob déficit hídrico, bem com avaliar a seleção precoce desses caracteres. Foram avaliados 25 genótipos de mandioca em delineamento experimental de blocos completos casualizados com quatro repetições. As variáveis peso de raízes (RW); número de raízes por planta (RN); diâmetro de raiz (RD); comprimento de raiz (RL); teor de matéria seca das raízes DMC; número de hastes por planta (SN); altura de plantas (PH); diâmetro de caule (SD); severidade de ácaros (MS); índice de colheita (HI) foram avaliadas sob condições de déficit hídrico em duas safras. Foram estimadas ainda herdabilidade no sentido amplo (h²) herdabilidade realizada ( ( h R 2 )), bem como o índice de coincidência (IC). As estimativas de acurácia variaram de 0,62 para RL a 0,86 para DMC. Foi possível identificar efeito significativo de genótipos para todos os caracteres, exceto para RW, RL e SD. Ocorreu interação genótipos x safras significativas para as variáveis SN, PH, SD e HI. As estimativas de (h²) variaram de 0,27 para RL a 0,79 para SN. As estimativas de índice de coincidência variaram de 100% a 25%. Foi observada correlação positiva entre todas as variáveis avaliadas, exceto MS, RN e SN com os demais caracteres. Os genótipos apresentaram desempenho coincidente nas duas safras para maioria das variáveis exceto para SN, PH, SD e HI. repetições RW (RW) (RN) RD (RD) (RL) DMC (SN) PH (PH) (SD) MS (MS) HI (HI h² (h² , )) IC. IC . (IC) 062 0 62 0,6 086 86 0,8 027 27 0,2 079 79 0,7 100 25% (RW (RN (RD (RL (SN (PH (SD (MS (h ) (IC 06 6 0, 08 8 02 07 7 10 1

ABSTRACT The aim of this study was to evaluate the effect of genotype x harvest interactions on different agronomic traits, the genetic correlation between traits in early selection under water stress, and early selection in cassava. 25 cassava genotypes were evaluated in a randomized block experimental design with four replications. The variables root weight (RW), root number per plant (RN), root diameter (RD), root length (RL), root dry matter content (DMC), stem number per plant (SN), plant height (PH), stem diameter (SD), mite severity (MS), and harvest index (HI) were evaluated under water stress conditions in two harvests. Broad-sense heritability (h²), realized heritability ( ( h R 2 )), and the index of coincidence (IC) were estimated. Accuracy estimates ranged from 0.62 for RL to 0.86 for DMC. A significant genotype effect was identified on all traits, except for RW, RL and SD. Significant genotype x harvest interactions occurred for the variables SN, PH, SD, and HI. (h²) estimates ranged from 0.27 for RL to 0.79 for SN. The estimates of the index of coincidence ranged from 100% to 25%. A positive correlation was observed between all variables under study, except for MS, RN and SN, and the other traits. The genotypes showed similar performance in the two harvests for most variables, except for SN, PH, SD, and HI. replications RW , (RW) RN, (RN) RD, RD (RD) RL, (RL) DMC, DMC (DMC) SN (SN) PH (PH) SD (SD) MS (MS) HI (HI Broadsense Broad sense h², h² )) IC (IC estimated 062 0 62 0.6 086 86 0.8 (h² 027 27 0.2 079 79 0.7 100 25% (RW (RN (RD (RL (DMC (SN (PH (SD (MS ) 06 6 0. 08 8 (h 02 07 7 10 1

RESUMO Jambu (Acmella oleracea) é uma hortaliça condimentar típica do Norte do Brasil que tem adquirido maior procura e notoriedade devido as suas propriedades sensoriais e bioativas, atribuídas à presença do composto espilantol. Estratégias para o manejo da planta têm sido buscadas, entretanto, a influência da suplementação com N sobre a biossíntese de espilantol no jambu em sistema hidropônico é desconhecida. O objetivo deste estudo foi avaliar o efeito do N na produção de biomassa, de pigmentos fotossintéticos, na concentração de N nas folhas e no teor de espilantol em jambu cultivado hidroponicamente. Estas plantas foram submetidas a seis concentrações de N na solução nutritiva (11, 13, 15, 17, 19 e 21 mmol L-1), utilizando delineamento inteiramente casualizado. Avaliaram-se a produção de biomassa fresca e seca das inflorescências, parte aérea (folhas e caules) e raiz, teor de N e pigmentos nas folhas e teor de espilantol nos diferentes órgãos da planta. As concentrações de N afetaram positivamente a produção de biomassa, de pigmentos fotossintéticos, a concentração de N nas folhas e o teor de espilantol nas inflorescências, parte aérea e raiz do jambu. O suprimento de 21 mmol L-1 N na solução nutritiva resultou em maior concentração de N nas folhas, promovendo maior produção de pigmentos fotossintéticos, biomassa da parte aérea e inflorescência. Suprimento médio de 17 mmol L-1 N resultou em maior síntese de espilantol nos órgãos do jambu. Portanto, suprimentos adequados de N devem ser considerados indispensáveis para o manejo nutricional do jambu cultivado em sistema hidropônico. Acmella oleracea bioativas buscadas entretanto desconhecida fotossintéticos hidroponicamente 11, 11 (11 13 15 1 2 L1, L1 L , L-1) casualizado Avaliaramse Avaliaram se inflorescências caules L- inflorescência Portanto (1 (

ABSTRACT Jambu (Acmella oleracea) is a typical culinary vegetable from Northern Brazil that has gained increased demand and recognition due to its sensory and bioactive properties attributed to the presence of spilanthol. Strategies for plant management have been explored; however, the influence of N supplementation on spilanthol biosynthesis in jambu in a hydroponic system is unknown. Thus, this study aimed to evaluate the effect of N on biomass production, photosynthetic pigments, leaf nitrogen concentration, and spilanthol content in jambu cultivated hydroponically. The plants were subjected to six concentrations of N in the nutrient solution (11, 13, 15, 17, 19, and 21 mmol L-1), using a completely randomized design with four replications, each consisting of one plant. Fresh and dry biomass of inflorescence, shoot (leaves and stems), and root, N and pigment content in the leaves, and spilanthol content in different plant organs were evaluated. The N concentrations positively affected biomass production, photosynthetic pigments, leaf N concentration, and spilanthol content in jambu inflorescence, shoot, and root. The supply of 21 mmol L-1 N in the nutrient solution resulted in a higher concentration of N in the leaves, leading to increased production of photosynthetic pigments, shoot biomass, and inflorescence. Conversely, a moderate supply of 17 mmol L-1 N resulted in a higher synthesis of spilanthol in the organs of the jambu plant. Therefore, appropriate nitrogen supplies should be considered an indispensable tool for the nutritional management of jambu cultivated in hydroponic systems. Acmella oleracea explored however unknown Thus pigments hydroponically 11, 11 (11 13 15 19 2 L1, L1 L 1 , L-1) replications inflorescence leaves stems, stems stems) root evaluated L- Conversely Therefore systems (1 (

Resumo Fundamento: O exercício físico tem sido considerado uma importante terapia não farmacológica para a prevenção e tratamento das doenças cardiovasculares. No entanto, seus efeitos na remodelação cardíaca leve não são claros. Objetivo: Avaliar a influência do exercício aeróbico sobre a capacidade funcional, estrutura cardíaca, função ventricular esquerda (VE) e expressão gênica das subunidades da NADPH oxidase em ratos com infarto do miocárdio pequeno (IM). Métodos: Três meses após a indução do IM, ratos Wistar foram divididos em três grupos: Sham; IM sedentário (IM-SED); e IM exercício aeróbico (IM-EA). Os ratos se exercitaram em uma esteira três vezes por semana durante 12 semanas. Um ecocardiograma foi realizado antes e após o treinamento. O tamanho do infarto foi avaliado por histologia e a expressão gênica por RT-PCR. O nível de significância para análise estatística foi estabelecido em 5%. Resultados: Ratos com IM menor que 30% da área total do VE foram incluídos no estudo. A capacidade funcional foi maior no IM-EA do que nos ratos Sham e IM-SED. O tamanho do infarto não diferiu entre os grupos. Ratos infartados apresentaram aumento do diâmetro diastólico e sistólico do VE, diâmetro do átrio esquerdo e massa do VE, com disfunção sistólica. A espessura relativa da parede foi menor no grupo IM-SED do que nos grupos IM-EA e Sham. A expressão gênica das subunidades NADPH oxidase NOX2, NOX4, p22phox e p47phox não diferiu entre os grupos. Conclusão: Infarto do miocárdio pequeno altera a estrutura cardíaca e a função sistólica do VE. O exercício aeróbico tardio pode melhorar a capacidade funcional e a remodelação cardíaca por meio da preservação da geometria ventricular esquerda. A expressão gênica das subunidades da NADPH oxidase não está envolvida na remodelação cardíaca, nem é modulada pelo exercício aeróbico em ratos com infarto do miocárdio pequeno.

Abstract Background: Physical exercise has been considered an important non-pharmacological therapy for the prevention and treatment of cardiovascular diseases. However, its effects on minor cardiac remodeling are not clear. Objective: To evaluate the influence of aerobic exercise on the functional capacity, cardiac structure, left ventricular (LV) function, and gene expression of NADPH oxidase subunits in rats with small-sized myocardial infarction (MI). Methods: Three months after MI induction, Wistar rats were divided into three groups: Sham; sedentary MI (MI-SED); and aerobic exercised MI (MI-AE). The rats exercised on a treadmill three times a week for 12 weeks. An echocardiogram was performed before and after training. The infarction size was evaluated by histology, and gene expression was assessed by RT-PCR. The significance level for statistical analysis was set at 5%. Results: Rats with MI lower than 30% of the LV total area were included in the study. Functional capacity was higher in MI-AE than in Sham and MI-SED rats. The infarction size did not differ between groups. Infarcted rats had increased LV diastolic and systolic diameter, left atrial diameter, and LV mass, with systolic dysfunction. Relative wall thickness was lower in MI-SED than in the MI-AE and Sham groups. Gene expression of the NADPH oxidase subunits NOX2, NOX4, p22phox, and p47phox did not differ between groups. Conclusion: Small-sized MI changes cardiac structure and LV systolic function. Late aerobic exercise is able to improve functional capacity and cardiac remodeling by preserving the left ventricular geometry. NADPH oxidase subunits gene expression is not involved in cardiac remodeling or modulated by aerobic exercise in rats with small-sized MI.

RESUMO A deriva da pulverização de inseticidas pode proporcionar a redução da eficiência de controle e perda para o ambiente. Desta forma, foi conduzido um estudo com o objetivo de estudar o efeito de diferentes pontas de pulverização e adição de adjuvantes na calda inseticida, em relação ao espectro de gotas e deriva em túnel de vento. Os experimentos foram conduzidos no delineamento inteiramente casualizado, com quatro repetições, no esquema fatorial 5 × 3, constituído por cinco caldas, uma apenas com água e outras quatro com inseticida tiametoxam + lambda-cialotrina (sem adjuvante, Oro-solve, Wetcit Gold e Orobor N1), combinadas com três pontas de pulverização (AXI, JFC e J3D). Foi avaliado o espectro das gotas, através do diâmetro mediano volumétrico, amplitude relativa e porcentagem do volume de gotas com diâmetro ≤ 100 µm. A deriva foi avaliada em túnel de vento, a diferentes distâncias: 5, 10 e 15 m. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey. Além disso, foram realizadas análises de componentes principais. A aplicação do inseticida com os adjuvantes combinados com as diferentes pontas de pulverização alterou o espectro de gotas e o risco da deriva. A ponta AXI e a J3D associada aos adjuvantes Oro-solve e Wetcit Gold apresentaram maiores diâmetros medianos volumétricos das gotas e deriva a 5 m.

ABSTRACT Insecticide spray drift can lead to reduced control efficiency and loss of product to the environment. Thus, we conducted a study to evaluate the effect of different spray nozzles and the addition of adjuvants in insecticide spray on the resulting droplet spectrum and wind tunnel drift. All experiments were conducted in a completely randomized design with four repetitions using a 5 × 3 factorial scheme. Five spraying solutions were studied; one contained only water and the other four comprised thiamethoxam + lambda-cyhalothrin (no adjuvant, Oro-solve, Wetcit Gold, and Orobor N1), in combination with three spray nozzles (AXI, JFC, and J3D). The droplet spectrum was evaluated through the volumetric median diameter, relative amplitude, and percentage of the droplet volume with diameter ≤ 100 µm. The drifts were evaluated in a wind tunnel at 5, 10, and 15 m. Data were subjected to analysis of variance and means were compared using the Tukey’s test. In addition, a principal component analysis was performed. Application of the insecticide with the adjuvants combined with the different nozzles changed the droplet spectrum and the risk of drift. The AXI nozzle and the J3D associated with the Oro-solve and Wetcit Gold adjuvants resulted in a greater volumetric median diameter of the droplets and drifts were observed at 5 m.

ABSTRACT Presence of toxic compounds in marine coastal waters has increased exponentially since Industrial Revolution. In this way, we aimed to evaluate biochemical and physiological changes occurring within Hypnea musciformis after short-term exposure to gasoline. Hypnea musciformis was cultivated without gasoline and then exposed to various concentrations of it (0.001 % - 1.0 %, v/v) for periods of 30 min, 1 h, 12 h and 24 h. A Pricncipal Compound Analysis of UV-vis spectral window (200-700 nm) was able to discriminate gasoline-exposed samples according to both exposure time and gasoline concentration. Changes in carotenoid profile composition were observed. Decreased carotenoid content was associated to gasoline exposure time, being lutein and trans-β-carotene the major compounds found. Higher gasoline concentrations negatively interfered with phenolic compounds accumulation. In addition, increased gasoline concentrations corresponded to decreased intracellular starch grains content as well as increased its deposition on cell wall external surface. Data obtained allow us to conclude that gasoline can damage Hypnea musciformis physiology and cell morphology. This is important, considering Hypnea musciformis carotenoids and phenolics are potential biomarkers of environmental stress investigated, as well as its increased cell wall thickness to avoid gasoline diffusion.

Abstract: This study aimed to estimate genetic parameters and correlations between morphological, agronomic and root quality traits of cassava plants, as well as to study cause and effect relationships through path analysis. A total of 814 genotypes were evaluated from 2011 to 2015. The joint analysis of the data was performed by the mixed models approach. The predicted genetic values of the genotypes were used to estimate the genetic correlations among as well the path analysis. The estimates of heritability of the genotype means ranged from 0.31 (commercial fresh root yield – CRY) to 0.62 (plant height - PLH). The highest genetic correlation coefficient estimates were observed for starch yield (STY) × total fresh root yield (FRY) (0.97). The results of the path analysis showed that FRY had the highest direct effect on STY, but the indirect selection based on FRY was not efficient to improve the gain of STY.

Abstract The objective of the present work was to use MAS in self-pollinated cassava populations for obtaining individuals with high inbreeding (f) aimed at rapid development of partial inbred lines. Three progenies (F0222, F1378 and F1662) were self-pollinated, generating a total of 233 S1 individuals. The progenies and the S1 individuals were evaluated in the seedlings and clonal evaluation trials (CETs). In the CET, plants were evaluated for the following traits: plant height (PH), root dry matter content (DMC), above ground yield (AGY), root (RY) and starch yield (SY). Twenty-seven microsatellites and five minisatellites were used to determine the level of inbreeding of the S1 individuals. Inbreeding (f) values varied from 0.15 to 0.89 within progenies, whereas most of the S1 individuals presented f values above 0.50. In average, 25% of the S1 individuals were selected, which resulted in a 38% inbreeding increase in the cassava progenies. In contrast, phenotypic selection showed no differences in inbreeding increase. Furthermore, there was no correlation between the level of inbreeding and agronomic traits. MAS was efficient for the identification and selection of cassava S1 individuals, with higher inbreeding values contributing to the decrease in the breeding cycles necessary to obtain new cassava inbred lines.

ABSTRACT The genus Passiflora is the most diversified of the Passifloraceae, and its palynology presents wide morphological variability. The objective of the study was to evaluate the pollen morphology of 18 Passiflora species in order to identify informative pollinic characteristics to contribute to the taxonomic classification of the genus. The morphology of the pollen grains and the exine structure were investigated using light microscopy and scanning electron microscopy. Differences in the pollen morphology were found in the studied species, mainly in terms of shape, pollen aperture and ornamentation pattern of the exine. Most of the species belonging to the subgenus Passiflora presented ornamented 6-syncolpate pollen grains with an oblate-spheroidal shape and an exine with large lumens. In the subgenus Decaloba the pollen grains were 6-colporate, 12-colpate and 12-colporate, with subprolate to prolate-spheroidal shape, as well as an exine with smaller lumen size and few ornamented. The pollen morphology of the species of the subgenera Passiflora and Decaloba has shown palynological characteristics that have specific diagnostic value, thus allowing a better understanding of the taxonomy of the genus Passiflora.

ABSTRACT New techniques of rapid multiplication of cassava (Manihot esculenta Crantz) have been developed, requiring technical support for large-scale use. This work main to evaluate the agronomic performance of plantlets obtained by leaf buds technique against stem cuttings in the field conditions. The work was conducted using the randomized block design in a factorial scheme with 3 varieties (BRS Kiriris, 98150-06, 9624-09) × 4 origins of the plantlets (conventional - stem cuttings of 20 cm length, leaf buds of the upper, middle and inferior stem part) × 2 agrochemicals (control and treated). There was a remarkable decrease in some agronomic traits that ranged from 23% (number of branches) to 62% (shoot weight) when using leaf buds plantlets. The treatment of plantlets with agrochemicals promoted significant increases in all traits, ranging from 26% (number of roots per plant) to 46% (shoot weight). The plantlets originating from leaf buds of the upper and middle parts were able to generate stem-like plants similar to stem-derived ones. Despite its lower agronomic performance under field conditions, multiplication by leaf buds may generate five times the number of propagules in comparison with the conventional multiplication, and therefore it could be a viable alternative for rapid cassava multiplication.

Neste trabalho é apresentado um estudo a respeito da análise da estrutura de substâncias redutoras de tensão superficial produzidas por Pseudomonas citronellolis 222A estimulado pela presença de ferro. Esta bactéria foi isolada de um solo que há 17 anos vem sendo utilizado para o tratamento de borra oleosa proveniente da indústria petroquímica e de refinaria de petróleo. O espectro de massa difere do espectro de P. aeruginosa, indicando que as substâncias redutoras de tensão superficial produzidas por P. citronellolis podem ser um novo tipo de biosurfactante.

In this work we investigated the structure of the iron-stimulated surface tension reducing substances produced by P. citronellolis 222A isolated from a 17-years old landfarming used for sludge treatment in petrochemical industries and oil refinery. Its mass spectrum differs from P. aeruginosa spectrum, indicating that the surface tension reducing substances produced by P. citronellolis can be a new kind of biosurfactant.

A mancha-angular causada pelo fungo Phaeoisariopsis griseola, apresenta grande importância na cultura do feijoeiro (Phaseolus vulgaris) no Brasil. O desenvolvimento de cultivares resistentes tem sido proposto como maneira eficaz, eficiente e econômica para o controle da doença. Um dos primeiros passos no programa de melhoramento visando resistência à mancha-angular é a identificação e seleção de fontes de resistência. Neste contexto, este trabalho objetivou a caracterização de 58 cultivares de feijoeiro quanto a reação às raças 31.17, 63.19, 63.23 e 63.55 de P. griseola. Os resultados mostraram que as cultivares Antioquia 8 e CAL 143, ambos de origem Andina, e Ecuador 299 e México 235, de origem Mesoamericana, apresentaram resistência às quatro raças testadas. As cultivares A 193 e Golden Gate 416 mostraram resistência a três das quatro raças testadas, podendo também, ser úteis em programas de melhoramento. Dentre as cultivares mais suscetíveis encontram-se as cultivares IPA 7419, AN 9022180, Bambuí, Compuesto Negro Chimaltengo, Guanajuato 10-A-5, Diamante Negro, Early Gallatin, Jamapa e Kentucky Wonder 780 e as cultivares de grãos tipo carioca AN 9022180, Aporé e Carioca 80. As novas fontes de resistência à mancha angular identificadas neste trabalho poderão ser utilizadas por programas de melhoramento do feijoeiro que visem a incorporação de genes de resistência de origem Andina ou Mesoamericana.

Angular leaf spot caused by the fungus Phaeoisariopsis griseola is a major disease of the common bean (Phaseolus vulgaris) in Brazil. The development of resistant cultivars has been proposed as an effective, efficient and economic way to control the disease. A breeding program aiming to develop cultivars resistant to angular leaf spot starts with the identification and selection of common bean resistance sources. The objective of this work was to characterize 58 common bean cultivars with respect to their reaction to P. griseola races 31.17, 63.19, 63.23 and 63.55. The results showed that cultivars Antioquia 8 and CAL 143, both of Andean origin and Ecuador 299 and Mexico 235 (both of Mesoamerican origin) showed resistance to all four tested races. The cultivars A 193 and Golden Gate 416 showed resistance to three of the four tested races, and can be used as sources of resistance to angular leaf spot. All the other cultivars were susceptible to at least one of the four tested races. The most susceptible were: IPA 7419, Bambuí, CNC, Guanajuato 10-A-5, Black Diamond, Early Gallatin, Jamapa, Kentucky Wonder 780, and the "carioca" type cultivars AN 9022180, Aporé and Carioca 80. The new resistance sources identified in this work could be used in common bean breeding programs aiming to incorporate Andean and Mesoamerican resistance genes to angular leaf spot.