Abstract Curcuma zedoaria is a traditional medicine used for various purposes in human life. The organic solvent extracts of this plant also exhibit antioxidant, antibacterial, anti-inflammatory, anti- cancer, anti-necrosis, and hepatoprotective properties. However, the prospect of employing this plant extract for skin whitening, particularly its molecular mechanism, has not been reported yet. Therefore, in this study, the total polyphenolic content, antioxidant activity, cytotoxicity, tyrosinase inhibitory, melanin inhibitory, and target gene expression using the ethyl acetate fraction of ethanolic extract from Curcuma zedoaria , were all examined. The TPC result of the sample was 42.18±2.25 mg GAE/g extract with impressive antioxidant activity. Half maximal inhibitory concentration value of 2,2-Diphenyl-1-picrylhydrazyl radical scavenging assay and Ferric reducing antioxidant power FRAP value at a concentration of 2.5 µg/mL were 49.06±0.29 µg/mL and 116±50 μM Fe2+/L, respectively. The extract had no cytotoxicity at 25 µg/mL, inhibited melanin production for 74.02±3.05% and tyrosinase for 137.45±1.38%. In particular, the extract demonstrated the ability to block the expression levels of four melanogenesis-related genes. Moreover, GCMS analysis results also detected 28 different compounds, with two new compounds accounting for a high proportion. These results suggested that C. zedoaria has a significant potential for application in skin-pigmentation treatment cosmetics. life antibacterial antiinflammatory, antiinflammatory anti inflammatory, inflammatory anti-inflammatory cancer antinecrosis, antinecrosis necrosis, necrosis anti-necrosis properties However whitening mechanism yet Therefore study content activity examined 4218225 42 18 2 42.18±2.2 GAEg GAE g 2,2Diphenyl1picrylhydrazyl 22Diphenyl1picrylhydrazyl Diphenylpicrylhydrazyl 2,2 Diphenyl 1 picrylhydrazyl 5 2. µgmL µg mL 4906029 49 06 0 29 49.06±0.2 11650 116 50 116±5 Fe2L FeL Fe2 L Fe Fe2+/L respectively 7402305 74 02 3 05 74.02±3.05 13745138 137 45 38 137.45±1.38% particular melanogenesisrelated melanogenesis related genes Moreover proportion C skinpigmentation pigmentation cosmetics 421822 4 42.18±2. 2Diphenyl1picrylhydrazyl 22 2, 490602 49.06±0. 1165 11 116± 740230 7 74.02±3.0 1374513 13 137.45±1.38 42182 42.18±2 49060 49.06±0 74023 74.02±3. 137451 137.45±1.3 4218 42.18± 4906 49.06± 7402 74.02±3 13745 137.45±1. 421 42.18 490 49.06 740 74.02± 1374 137.45±1 42.1 49.0 74.02 137.45± 42. 49. 74.0 137.45 74. 137.4 137.

Abstract Citrus peel extract has been observed with many beneficial-promoting effects. Pomelo (Citrus grandis (L) Osbeck) peel extracts (PPE) from the flavedo and albedo of two varieties namely Long Co Co and Thanh Kieu were investigated. In this study, the qualitative analysis indicated the presence of phytochemical groups including alkaloids, flavonoids, saponins, and tannin in the PPE from the flavedo and albedo. The PPE from the Thanh Kieu flavedo was found to possess the highest antioxidant activity at the EC50 values of 60.89 ± 0.31 µg/mL, 60.69 ± 0.21 µg/mL, and 24.16±0.06 µg/mL for DPPH, ABTS, and FRAP assay. However, the Long Co Co albedo- PPE induced better inhibitory effect against α-amylase (EC 232-560-9) and α-glucosidase (EC 232-604-7) with respect to EC50 values of 3.59 ± 0.02 mg/mL and 80.77 ± 0.34 µg/mL. This result suggested potential in the anti-hyperglycemic effect of pomelo peel extract from the flavedo and albedo that can serve as a nutraceutical in food and pharmaceutical industries.

Abstract: Novel biomarkers for screening, diagnosis and monitoring the treatment of nasopharyngeal carcinoma (NPC), one of the most common cancers in Vietnam, are urgently required. Increasing evidence suggests that microRNA-141 (miR-141) is associated with NPC, owing to its ability to affect the expression of genes that modulate tumorigenesis. Unfortunately, research on miR-141 expression in Vietnamese patients is limited. Therefore, the objective of the current study was to evaluate miR-141 expression and assess whether miR-141 might be a potential biomarker for diagnosis of NPC in Vietnamese patients. Total RNA isolated from 40 NPC biopsy samples and 37 non-cancerous samples was analyzed by quantitative reverse-transcription PCR. The miR-141 expression levels were compared between NPC biopsy and non-cancerous samples. The frequency of miR-141 detection was 37.50% and 10.80% in the NPC and non-cancerous samples, respectively (p = 0.0143). The miR-141 expression was 5.27 times higher in tumor samples than non-cancerous samples. Additionally, the RR (Relative risk) and OR (Odds ratio) were 1.83 (95%CI = 1.2576–2.6675, p = 0.0016) and 4.95 (95%CI = 1.4625–16.7541, p = 0.01), respectively. In conclusion, miR-141 was up-regulated in the biopsy samples and thus may be a potential biomarker for NPC in the Vietnamese population.