ABSTRACT. Food production in a sustainable way is based on the association between animals and vegetables to minimize costs, environmental impact, and the use of insums in the breeding process. The objective of this work was to evaluate the effect of the inclusion of pumpkin seed flour (Cucurbita pepo L.) in the diet on the performance of early-stage pigs and the reduction of endoparasites in a commercial rearing system. The experimental design was completely randomized, with three treatments and six replicates, and the experimental unit consisted of two animals, totaling 36 animals. The treatments were the basal diet and two levels of inclusion of pumpkin seed: 5 and 10%. The animals were of the Agroceres line, with 42 days of age and an average weight of 12 kg. The animals and rations were weighed at the beginning and at the end of the 35-day experimental period to obtain the performance variables. A bromatological analysis was performed to evaluate the composition of pumpkin seed and for parasitic analysis, feces were collected individually from the animal. There was no significant effect for the Tukey test at the level of 5% probability, of the inclusion of pumpkin seed flour on in the parameters of weight gain and feed conversion. Was observed the reduction of helminth eggs present in feces using egg count and oocysts per gram of feces (OPG), performed at the beginning and end of the experiment. It is indicated the inclusion of 10% of pumpkin flour in the feeding of pigs in the initial phase by reducing contamination by verminosis and does not alter animal performance. ABSTRACT costs impact process Cucurbita L. L earlystage early stage system randomized replicates 3 10 line 4 1 kg 35day day 35 variables probability conversion OPG, OPG , (OPG) experiment (OPG

Abstract This study compares the effects of virus-cell interactions among SARS-CoV-2 variants of concern (VOCs) isolated in Brazil in 2021, hypothesizing a correlation between cellular alterations and mortality and between viral load and transmissibility. For this purpose, reference isolates of Alpha, Gamma, Zeta, and Delta variants were inoculated into monolayers of Vero-E6 cells. Viral RNA was quantified in cell supernatants by RT‒PCR, and infected cells were analyzed by Transmission Electron Microscopy (TEM) for qualitative and quantitative evaluation of cellular changes 24, 48, and 72 hours postinfection (hpi). Ultrastructural analyses showed that all variants of SARS-CoV-2 altered the structure and function of mitochondria, nucleus, and rough endoplasmic reticulum of cells. Monolayers infected with the Delta variant showed the highest number of modified cells and the greatest statistically significant differences compared to those of other variants. Viral particles were observed in the cytosol and the cell membrane in 100 % of the cells at 48 hpi. Alpha showed the highest mean particle diameter (79 nm), and Gamma and Delta were the smallest (75 nm). Alpha and Gamma had the highest particle frequency per field at 48 hpi, while the same was observed for Zeta and Delta at 72 hpi and 24 hpi, respectively. The cycle threshold of viral RNA varied among the target protein, VOC, and time of infection. The findings presented here demonstrate that all four VOCs evaluated caused ultrastructural changes in Vero-E6 cells, which were more prominent when infection occured with the Delta variant. viruscell virus SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- (VOCs 2021 transmissibility purpose VeroE6 VeroE Vero E6 E Vero-E RTPCR RT PCR RT‒PCR TEM (TEM 7 . (hpi) mitochondria nucleus 10 4 79 (7 nm, nm , nm) 75 nm. respectively protein VOC SARS-CoV 202 (hpi 1 ( 20

ABSTRACT. Bahiagrass (Paspalum notatum Flügge) is an important forage in South America and the United States because of its high palatability, resistance to trampling and grazing, and tolerance to cold, but it exhibits low seed yield and poor seed quality. Previous studies reported improvements in forage production and nutritive value in hybrids and ecotypes; however, information about seed yield and quality in forage bahiagrass is limited. This study aimed to characterize the seed yield and quality of nine P. notatum intraspecific hybrids and three controls: P. notatum ecotypes V4 and Bagual and cultivar Pensacola. Inflorescence density, 1,000-seed weight, seed yield and germination rate decreased in year 2 influenced by weather conditions and ergot. Seed yield ranged from 139 (Pensacola) to 1,158 (Bagual) kg ha-1 among all entries, where Bagual, C18, and V4 produced more than 974 kg ha-1, which was approximately seven times more than Pensacola. Bagual, C18, V4, 336, C15, 225, and D3 showed germination rates greater than 83%. In conclusion, Bagual had the highest seed yield and germination rate, whereas hybrids C18 and 336 showed high seed yield, 1,000-seed weight, and germination rate in both years. Bagual, C18, and 336 should be used in future breeding programs to improve seed production traits. Our study revealed that selection for seed production traits can result in improvements in seed yield and quality in bahiagrass. ABSTRACT Paspalum Flügge palatability grazing cold however limited P controls V Pensacola density 1,000seed 1000seed 1,000 1 000 weight ergot 13 (Pensacola 1158 158 1,15 (Bagual ha1 ha ha- entries C 97 ha1, 1, C15 225 D 83 83% conclusion C1 33 years 000seed 1000 1,00 00 115 15 1,1 9 22 8 3 100 1,0 0 11 10

RESUMEN Objetivo: analizar las percepciones de los profesionales de la Atención Primaria de Salud sobre el acceso de las personas con tuberculosis pulmonar a los programas gubernamentales de apoyo social y transferencia de ingresos. Métodos: estudio multicéntrico/cualitativo, realizado en Unidades de Salud de la Familia en cuatro capitales brasileñas: Belém/Pará, Campo Grande/Mato Grosso do Sul, Recife/Pernambuco y Rio de Janeiro/Rio de Janeiro. Participaron 58 profesionales (trabajadores sociales, odontólogos, enfermeros, farmacéuticos, médicos y técnicos de enfermería) que brindaron asistencia a personas con tuberculosis pulmonar. Se realizaron entrevistas individuales y se utilizó la técnica de análisis de contenido. Resultados: entre los participantes, 45/77,6% eran mujeres y 33/56,9% tenían entre 25 y 40 años. Se organizaron dos categorías temáticas, demostrando las percepciones sobre las posibilidades de acceso a los programas gubernamentales por parte de las personas con tuberculosis pulmonar en situación de vulnerabilidad y los obstáculos inherentes a este contexto. Consideraciones finales: es necesario avanzar en mejorar el acceso de los pacientes a los programas sociales. Objetivo ingresos Métodos multicéntricocualitativo multicéntrico cualitativo multicéntrico/cualitativo brasileñas BelémPará Belém Pará Belém/Pará GrandeMato Grande Mato Sul RecifePernambuco Recife Pernambuco JaneiroRio Janeiro 5 trabajadores sociales odontólogos enfermeros farmacéuticos enfermería contenido Resultados participantes 45776 45 77 6 45/77,6 33569 33 56 9 33/56,9 2 4 años temáticas contexto finales 4577 7 45/77, 3356 3 33/56, 457 45/77 335 33/56 45/7 33/5 45/ 33/

ABSTRACT Objective: to analyze Primary Health Care professionals’ perceptions about the access of people with pulmonary tuberculosis to government social support and income transfer programs. Methods: multicenter/qualitative study, carried out in Family Health Units in four Brazilian capitals: Belém/Pará, Campo Grande/Mato Grosso do Sul, Recife/Pernambuco and Rio de Janeiro/Rio de Janeiro. Fifty-eight professionals participated (social workers, dentists, nurses, pharmacists, physicians and nursing technicians), who provided assistance to people with pulmonary tuberculosis. Individual interviews were conducted, and the content analysis technique was used. Results: among the participants, 45/77.6% were women and 33/56.9% were between 25 and 40 years old. Two thematic categories were organized, demonstrating the perceptions about the possibilities of access to government programs by people with pulmonary tuberculosis in vulnerable situations and the obstacles inherent to this context. Final considerations: it is necessary to move forward in improving patient access to social programs. Objective Methods multicenterqualitative multicenter qualitative study capitals BelémPará Belém Pará Belém/Pará GrandeMato Grande Mato Sul RecifePernambuco Recife Pernambuco JaneiroRio Janeiro Fiftyeight Fifty eight workers dentists nurses pharmacists technicians, technicians , technicians) conducted used Results participants 45776 45 77 6 45/77.6 33569 33 56 9 33/56.9 2 4 old organized context considerations 4577 7 45/77. 3356 3 5 33/56. 457 45/77 335 33/56 45/7 33/5 45/ 33/

RESUMO Objetivo: analisar as percepções dos profissionais da Atenção Primária à Saúde sobre o acesso de pessoas com tuberculose pulmonar aos programas sociais governamentais de suporte e de transferência de renda. Métodos: estudo multicêntrico/qualitativo, realizado em Unidades de Saúde da Família de quatro capitais brasileiras: Belém/Pará, Campo Grande/Mato Grosso do Sul, Recife/Pernambuco e Rio de Janeiro/Rio de Janeiro. Participaram 58 profissionais (assistentes sociais, dentistas, enfermeiros, farmacêuticos, médicos e técnicos de enfermagem), que prestavam assistência às pessoas com tuberculose pulmonar. Foram realizadas entrevistas individuais, e utilizou-se a técnica de análise de conteúdo. Resultados: entre os participantes, 45/77,6% eram mulheres e 33/56,9% tinham entre 25 e 40 anos. Organizaram-se duas categorias temáticas, demonstrando as percepções acerca das possibilidades de acesso aos programas governamentais por pessoas com tuberculose pulmonar em situação de vulnerabilidade e os obstáculos inerentes a esse contexto. Considerações finais: é necessário avançar na melhoria do acesso dos doentes aos programas sociais. Objetivo renda Métodos multicêntricoqualitativo multicêntrico qualitativo multicêntrico/qualitativo brasileiras BelémPará Belém Pará Belém/Pará GrandeMato Grande Mato Sul RecifePernambuco Recife Pernambuco JaneiroRio Janeiro 5 assistentes dentistas enfermeiros farmacêuticos enfermagem, enfermagem , enfermagem) individuais utilizouse utilizou se conteúdo Resultados participantes 45776 45 77 6 45/77,6 33569 33 56 9 33/56,9 2 4 anos Organizaramse Organizaram temáticas contexto finais 4577 7 45/77, 3356 3 33/56, 457 45/77 335 33/56 45/7 33/5 45/ 33/

RESUMO. Demência rapidamente progressiva é uma condição rara, cujos diagnósticos diferenciais incluem intoxicação por drogas como lítio, podendo causar importante disfunção cognitiva. A suspeita clínica, a confirmação laboratorial e a interrupção do uso medicamentoso são elementos fundamentais em seu diagnóstico e manejo. Trata-se de paciente feminina, de 66 anos de idade, que apresentou quadro demencial após intoxicação por lítio. Tal quadro foi acompanhado de sintomas parkinsonianos, além de Tomografia por Emissão de Pósitrons com 18F-Fluodeoxiglicose (18F-FDG PET/CT) compatível com padrão “Alzheimer-like”. Houve melhora objetiva de parâmetros clínicos e imaginológicos após a interrupção do uso medicamentoso. A intoxicação por lítio é uma causa potencialmente reversível de demência rapidamente progressiva. A descontinuação da droga deve ser considerada também em pacientes com níveis normais do metal no sangue se alterações cognitivas forem encontradas. Imagens 18F-FDG PET/CT podem demonstrar achados sugestivos de doença de Alzheimer na intoxicação aguda e parecem ser um método útil no seguimento.

ABSTRACT. Rapidly progressive dementia (RPD) is a rare neurological disorder. Drug toxicity is among the differential diagnoses, including the use of lithium, in which an overdosage might cause cognitive dysfunction. Clinical suspicion, laboratory confirmation, and drug interruption are key points in the management of lithium intoxication. We described a 66-year-old female patient under treatment with lithium who developed an RPD associated with parkinsonian symptoms. 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) showed an “Alzheimer-like” pattern, while cerebrospinal fluid biomarkers for the disease were negative. There was a significant clinical and radiological improvement after lithium interruption. Lithium intoxication is a potentially reversible cause of RPD, as demonstrated in this case report. Drug discontinuation should be considered even in patients with normal levels of this metal, if cognitive impairment is detected. 18F-FDG PET/CT images may show an “Alzheimer-like” image pattern in acute intoxication and are useful for monitoring these patients.

RESUMO O conhecimento da taxa de infiltração de água no solo é fundamental para estudos hidrológicos, planejamento e projeto de sistemas de irrigação e drenagem, entre outras aplicações. Diversos trabalhos têm sido realizados em parcelas com e sem cobertura vegetal, objetivando identificar a influência da cobertura no processo de infiltração de água no solo. Porém, poucos trabalhos tem abordado a relação entre as taxas de infiltração com e sem cobertura vegetal. Avaliamos, neste trabalho, a capacidade de iteração entre as taxas de infiltração com e sem cobertura vegetal, buscando identificar potenciais correlações. O objetivo principal deste trabalho foi de propor um modelo empírico inovador, de fácil utilização, que permita a conversão de taxas de infiltração em sistemas com cobertura vegetal em taxas de infiltração sem cobertura e vice versa. A base de dados foi composta por 142 testes, sendo 71 testes com cobertura e 71 testes sem cobertura vegetal, compreendendo 6 diferentes tipos de solo e 18 tipos de coberturas e manejo. O modelo proposto tomou como base o modelo Kostiakov-Lewis, apresentando desempenho semelhante a outros modelos de infiltração, sendo eficaz em qualquer sistema de plantio e cobertura vegetal.

ABSTRACT Soil water infiltration rates are essential for hydrological studies, planning and design of irrigation and drainage systems, among other applications. Various studies have been carried out in plots with and without vegetation cover, aiming to identify the influence of the cover on the water infiltration process in soil. However, a few works have addressed the relationship between infiltration rates of a plot with and without vegetation cover. Here we investigated the ability to iterate between infiltration rates with and without vegetation cover, seeking to identify potential correlations. We propose an innovative and easy-to-use empirical model that allows the conversion of infiltration rates in systems with vegetation cover into infiltration rates without coverage and vice versa. Altogether, we used a dataset comprising 142 rainfall simulation experiments under plots with and without cover, including 6 different types of soil and 18 types of land cover and management. The proposed model was based on the Kostiakov-Lewis model, presenting performance similar to other infiltration models, which is effective in a variety of planting and vegetation cover systems.

ABSTRACT The objective of this work was to describe morphology and grouping of Paspalum notatum accessions, based on multicategorical data which discards the redundant variables for quantification of genetic diversity. We also tested the hypothesis that geographical distance was correlated with morphological divergence. In our study, multivariate analyzes successfully demonstrated the geographic and morphological variability of the P. notatum accessions characterized. Many of these evaluated accessions can be included in future genetic improvement programs. Based on two methodologies for discarding variables, it was possible to identify the potentially important morphological characteristics from genetic diversity studies and characterize new accessions aimed at improving forage and seed production. The methodologies used to discard variables are biometric tools that can be used successfully in future plant breeding programs, especially when a large number of traits and accessions are being evaluated. Although significant, geographic distance had a low association with morphological traits. This indicated the need to use other characteristics, such as forage and seed yield, in addition to molecular analysis. Our analyzes showed genetic variability in P. notatum for all the characteristics studied.

RESUMO Complicações oftalmológicas de cirurgias não oculares são raras, mas podem levar a condições irreversíveis de baixa acuidade visual. Em geral são associadas à cirurgia de coluna, coração ou pescoço, mas podem ocorrer após procedimentos em outros sistemas. As principais causas são neuropatias ópticas isquêmicas, oclusões vasculares, lesões corticais, e glaucoma agudo de ângulo fechado. Relatamos dois casos de baixa acuidade visual súbita, secundária a oclusões vasculares, após procedimentos cirúrgicos gastrointestinais. No primeiro caso, um paciente de 57 anos foi internado de forma eletiva para reconstrução do cólon após colostomia de Hartmann. Evoluiu com complicações nos períodos intra- e pós-operatório, e necessitou de outras cirurgias complementares. Um mês depois apresentou baixa acuidade visual bilateral súbita, indolor e não altitudinal, e foi diagnosticado como papiloflebite, com resolução espontânea na evolução. O segundo caso, uma paciente de 69 anos, sem comorbidades, foi submetida à ressecção do reto por suspeita de tumor maligno e, no terceiro dia de pós-operatório, evoluiu com dor e baixa acuidade visual bilateral, secundária a glaucoma agudo de ângulo fechado, e oclusão de ramo da artéria retiniana no olho direito; tratada com iridotomia e colírios hipotensores, com recuperação parcial da visão. O objetivo do artigo é discutir os mecanismos etiológicos das doenças relatadas, e apresentar uma revisão da literatura.

ABSTRACT Ophthalmologic complications of nonocular surgeries are rare events, but can lead to irreversible conditions of low visual acuity. They are often associated with spine, heart and neck surgery, however they can occur after procedures on other systems. The main local causes are ischemic optic neuropathies, vascular occlusions, cortical lesions, and acute angle-closure glaucoma. We report two cases of sudden low visual acuity secondary to vascular occlusions after gastrointestinal procedures. In the first case, a 57-year-old patient electively admitted for colon reconstruction after Hartmann’s colostomy, progressed with intra- and postoperative complications and required subsequent complementary surgeries. Once month later he presented with sudden bilateral low visual acuity, painless and non-altitudinal, and was diagnosed as papillophlebitis, which resolved spontaneously with the progression of the condition. The second case, a 69-year-old patient with no comorbidities underwent rectal resection due to suspected malignant tumor, and progressed on the third postoperative day, with pain and bilateral low visual acuity secondary to acute angle-closure glaucoma, and branch retinal artery occlusion in right eye; treated with iridotomy and ocular hypotensive eye drops, with only slight recovery of vision. The article aims to discuss the etiological mechanisms of the reported conditions and present a literature review.

RESUMO Objetivo: avaliar o risco cardiovascular entre trabalhadores de enfermagem de um hospital público. Método: estudo seccional, com 324 trabalhadores de enfermagem, utilizando-se questionário composto por dois blocos de informações. O primeiro abrangeu questões relacionadas às características sociodemográficas e laborais, de saúde, e o segundo, o Escore de Risco de Framingham Revisado (ERF), para estratificar o risco cardiovascular. Para avaliar o estresse psicossocial no trabalho, utilizou-se a Escala Sueca de Demanda Controle Social. Resultados: o fator de risco modificável para DCVs mais prevalente foi a circunferência de cintura (75,9%), seguida de sobrepeso (43,8%), obesidade (29,3%), etilismo (21,9%), colesterol da lipoproteína de densidade baixa (LDL) > 130 (20,7%) e Hipertensão Arterial Sistêmica (20,4%). Conclusão: verificou-se que 96% dos trabalhadores apresentaram baixo risco para desenvolverem doenças cardiovasculares nos próximos dez anos, e trabalhadores homens com idade >40 anos jornada de trabalho menor apresentam maiores chances de apresentar doença cardiovascular.

ABSTRACT Objective: to assess cardiovascular risk among nursing workers at a public hospital. Method: a cross-sectional study, with 324 nursing workers, using a questionnaire composed of two information blocks. The first covered issues related to sociodemographic and occupational and health characteristics, and the second, the Revised Framingham Risk Score (FRS), to stratify cardiovascular risk. To assess work-related psychosocial stress, the Swedish Social Control Demand Scale was used. Results: the most prevalent modifiable risk factor for CVDs was waist circumference (75.9%), followed by overweight (43.8%), obesity (29.3%), alcohol consumption (21.9%), lipoprotein cholesterol low density (LDL) > 130 (20.7%) and hypertension (20.4%) Conclusion: it was found that 96% of workers had a low risk of developing cardiovascular disease in the next ten years, and male workers aged >40 years with shorter working hours are more likely to have cardiovascular disease.

RESUMEN Objetivo: evaluar el riesgo cardiovascular en trabajadores de enfermería de un hospital público. Método: estudio transversal, con 324 trabajadores de enfermería, utilizando un cuestionario compuesto por dos bloques de información. El primero abordó cuestiones relacionadas con las características sociodemográficas y laborales y de salud, y el segundo, el Puntaje de Riesgo de Framingham revisada (ERF), para estratificar el riesgo cardiovascular. Para evaluar el estrés psicosocial en el trabajo se utilizó la Escala Sueca de Demanda de Control Social. Resultados: factor de riesgo modificable más prevalente para ECV fue el perímetro de cintura (75,9%), seguido del sobrepeso (43,8%), obesidad (29,3%), consumo de alcohol (21,9%), colesterol unido a lipoproteínas de baja densidad (LDL) > 130 (20,7%) e Hipertensión Arterial Sistémica (20,4%). Conclusión: se encontró que el 96% de los trabajadores tenían bajo riesgo de desarrollar enfermedad cardiovascular en los próximos diez años, y los trabajadores de sexo masculino > 40 años con jornadas laborales más cortas tienen mayor probabilidad de tener enfermedad cardiovascular.

RESUMO: Este estudo teve como objetivo avaliar a estrutura da vegetação e o desempenho de novilhas em recria e vacas de descarte em pastagens naturais manejadas em sistema de pastejo rotacionado com lotes “ponta” e “rapador” no período primavera-verão. Os tratamentos foram diferentes intervalos de descanso de 402 e 252 graus-dia (GD), os quais favorecem o crescimento de gramíneas de diferentes grupos funcionais. O delineamento experimental foi em blocos ao acaso com dois tratamentos e três repetições. Os animais de teste foram 24 novilhas no grupo “ponta” e 24 vacas no grupo “rapador”. No estrato entre touceiras, as alturas do pasto maiores que 16 cm foram predominantes em ambos os tratamentos, sendo 1.3 vezes mais frequente no tratamento 402 GD em relação ao tratamento 252 GD. A taxa de lotação média foi 22% maior no tratamento com 252 GD. Embora não tenham sido observadas diferenças para o ganho médio diário de novilhas (0,227 kg.dia-1) e vacas (0,336 kg.dia-1) entre os tratamentos, o ganho de peso por área no período experimental foi maior no tratamento 252 GD. Apesar do sistema de manejo com lotes “ponta” e “rapador” não proporcionar mudanças significativas na estrutura da pastagem, ele permite atingir metas de desempenho satisfatórias em áreas de pastagens naturais.

ABSTRACT: This study evaluated the structure of vegetation and the performance of rearing heifers and cull cows in Campos grasslands managed in the rotational stocking method with first-last stocking in the spring-summer period. The treatments were different rest intervals of 402 and 252 degrees-day, which favor the growth of different functional groups of grasses. A completely randomized block design with two treatments and three repetitions was used. The test animals were 24 heifers in the “first” group and 24 cows in the “last” group. In the stratum between tussock grasses, sward heights greater than 0.16 m were predominant in both treatments, it was 1.3 times more frequent in the 402 DD treatment compared to the 252 DD treatment. Average stocking rate was 22% higher in the 252 DD treatment. Although, no differences were observed for the average daily gain of heifers (0.227 kg.day-1) and cows (0.336 kg.day-1) between treatments, the weight gain per area in the experimental period was greater in the 252 DD treatment. Despite the management system with first and last stoking does not provide significant changes in the structure of the pasture, it allowed to achieve satisfactory performance goals in areas of Campos grassland.

RESUMO Objetivos: analisar as iniciativas sociais governamentais voltadas para as pessoas com tuberculose e as possibilidades de acesso aos programas sociais governamentais e de transferência de renda, na percepção dos gestores de programas de tuberculose. Métodos: estudo descritivo, qualitativo, com 19 gestores de Belém, Recife, Campo Grande e Rio de Janeiro. Utilizou-se a análise de conteúdo temática. Resultados: não há suporte social governamental específico para pessoas com tuberculose; os benefícios são destinados às pessoas em vulnerabilidade social. Existem parcerias entre as instituições da rede social secundária da saúde, assistência social e instituições comunitárias. Considerações Finais: o apoio das instâncias oficiais é importante para o controle da tuberculose, contudo o perfil de vulnerabilidade das pessoas é fator determinante para o acesso ao/destinação dos recursos desses programas governamentais de apoio social.

ABSTRACT Objectives: to analyze government social initiatives aimed at people with tuberculosis and the possibilities of access to government social programs and income transfers through the perception of tuberculosis program managers. Methods: descriptive, qualitative study with 19 managers from Belém, Recife, Campo Grande, and Rio de Janeiro, Brazil. Thematic content analysis was used. Results: there is no specific government social support for people with tuberculosis; the benefits are intended for people in social vulnerability. There are partnerships between the institutions of the secondary social healthcare network, social assistance, and community institutions. Final Considerations: the support of official bodies is important for the control of tuberculosis; however, the profile of people’s vulnerability is a determining factor for access to/destination of resources from these government social support programs.

RESUMEN Objetivos: analizar las iniciativas sociales gubernamentales dirigidas a las personas con tuberculosis y las posibilidades de acceso a los programas sociales gubernamentales y de transferencia de renta, en la percepción de los gestores de programas de tuberculosis. Métodos: estudio descriptivo, cualitativo, con 19 gestores de Belém, Recife, Campo Grande y Rio de Janeiro. Se utilizó el análisis de contenido temático. Resultados: no hay soporte social gubernamental específico para personas con tuberculosis; los beneficios son destinados a las personas en vulnerabilidad social. Hay colaboraciones entre las instituciones de la red social secundaria de salud, asistencia social e instituciones comunitarias. Consideraciones Finales: el apoyo de las instancias oficiales es importante para el control de la tuberculosis, con todo el perfil de vulnerabilidad de las personas es factor determinante para el acceso al/destinación de los recursos de esos programas gubernamentales de apoyo social.

BACKGROUND The coronaviruses (CoVs) called the attention of the world for causing outbreaks of severe acute respiratory syndrome (SARS-CoV), in Asia in 2002-03, and respiratory disease in the Middle East (MERS-CoV), in 2012. In December 2019, yet again a new coronavirus (SARS-CoV-2) first identified in Wuhan, China, was associated with a severe respiratory infection, known today as COVID-19. This new virus quickly spread throughout China and 30 additional countries. As result, the World Health Organization (WHO) elevated the status of the COVID-19 outbreak from emergency of international concern to pandemic on March 11, 2020. The impact of COVID-19 on public health and economy fueled a worldwide race to approve therapeutic and prophylactic agents, but so far, there are no specific antiviral drugs or vaccines available. In current scenario, the development of in vitro systems for viral mass production and for testing antiviral and vaccine candidates proves to be an urgent matter. OBJECTIVE The objective of this paper is study the biology of SARS-CoV-2 in Vero-E6 cells at the ultrastructural level. METHODS In this study, we documented, by transmission electron microscopy and real-time reverse transcription polymerase chain reaction (RT-PCR), the infection of Vero-E6 cells with SARS-CoV-2 samples isolated from Brazilian patients. FINDINGS The infected cells presented cytopathic effects and SARS-CoV-2 particles were observed attached to the cell surface and inside cytoplasmic vesicles. The entry of the virus into cells occurred through the endocytic pathway or by fusion of the viral envelope with the cell membrane. Assembled nucleocapsids were verified inside rough endoplasmic reticulum cisterns (RER). Viral maturation seemed to occur by budding of viral particles from the RER into smooth membrane vesicles. MAIN CONCLUSIONS Therefore, the susceptibility of Vero-E6 cells to SARS-CoV-2 infection and the viral pathway inside the cells were demonstrated by ultrastructural analysis.

ABSTRACT The objective of this work was to determine Paspalum notatum genotypes appropriate for the establishment of mixtures with temperate legumes and compare the productivity of mixtures with mineral-fertilized systems along two years. Four hybrids of P. notatum, ecotype Bagual and cv. Pensacola, were either subjected to mixtures with white clover (Trifolium repens) and birdsfoot trefoil (Lotus corniculatus) or fertilized with 0, 60, 120, 240, and 480 kg N ha−1 year−1. P. notatum leaf blades and stems and herbage accumulation denoted a management × year interaction. Herbage accumulation varied between the mixtures and fertilized systems in first year, while in the second year, herbage accumulation of mixed systems employing the B26, B43, C22, C9, and Bagual genotypes were similar to that of systems fertilized with 120 and 240 kg N ha−1. Hybrids B26 and C9 and ecotype Bagual, once made available to producers, could be indicated for mixtures with temperate legumes because they are superior to cv. Pensacola. Herbage accumulation in mixtures involving white clover plus birdsfoot trefoil and P. notatum genotypes is similar to that of mineral nitrogen-fertilized systems with 240 kg N ha−1, emphasizing the viability of mixed between these species.

RESUMO A sífilis é uma infecção sexualmente transmissível causada pela espiroqueta Treponema pallidum. A sífilis ocular pode ocorrer em qualquer estágio da doença, chegando a 10% na forma secundária e a 2% a 5% em sua forma terciária. A uveíte é a manifestação ocular mais comum, ocorrendo em 0,4% a 8% dos pacientes com a doença sistêmica. A coriorretinite é a manifestação mais comum do segmento posterior. Apresentamos o caso de um paciente do sexo masculino, 53 anos, com queixa de baixa acuidade visual e nictalopia há ٣ anos. Seu exame físico revelou lentificação dos reflexos pupilares, celularidade no vítreo anterior, papilas fisiológicas, atenuação arteriolar, redução do reflexo foveal, atrofia difusa do epitélio pigmentar da retina, acúmulo punctato de pigmento em regiões peripapilar e perivascular e coriorretinite periférica. Eletrorretinograma de campo total extinto em ambos os olhos. O teste treponêmico foi positivo. Foi previamente diagnosticado como portador de retinose pigmentar, evoluindo com cegueira, a despeito do tratamento correto instituído. Esse caso mostra a sífilis como importante causadora de cegueira. Casos atípicos de retinose pigmentar devem alertar o oftalmologista para causas de pseudorretinose pigmentar, como a sífilis.

ABSTRACT Syphilis is a sexually transmitted infection caused by the spirochete Treponema pallidum. Ocular involvement can occur at any time, and it may affect 10% of patients in the secondary stage, and from 2% to 5% in the tertiary stage. Uveitis is the most common presentation of ocular syphilis, affecting 0.4% to 8% of patients with systemic disease. Chorioretinitis is the most common posterior alteration. We present the case of a 53-year-old male patient, presenting with bilateral low visual acuity and nyctalopia for 3 years. His physical examination revealed decreased pupillary reflex, anterior vitreous cells, physiologic papillae, arteriolar attenuation, reduced foveal reflex, diffuse retinal pigment epithelium atrophy, peripapillary and perivascular punctate pigment accumulation and peripheral chorioretinitis. Full-field electroretinogram was extinct in both eyes. Treponemal syphilis test was positive. Previously diagnosed as retinitis pigmentosa, evolved to blindness, despite proper treatment. Our case shows syphilis as a significant cause of blindness. Atypical presentations of retinitis pigmentosa must warn ophthalmologists to etiologies of pseudoretinitis pigmentosa, such as syphilis.

ABSTRACT The objective of this study was to evaluate 94 Paspalum notatum genotypes over two growing seasons to estimate genetic dissimilarity through agronomic traits and the distance between genotypes. This information is used to create an ideotype from the best averages obtained for the set of characteristics evaluated. Seven apomitic, three sexual, and 81 hybrid genotypes were compared with native genotypes “André da Rocha”, “Bagual”, and cultivar “Pensacola” as controls. There is genetic variability in P. notatum for the studied characteristics, and distinct genotypes with superior characteristics can be used in new combinations between apomictic and sexual plants to obtain hybrids. The characters with the greatest relative contribution to the dissimilarity between the genotypes were tiller density, stem dry mass, and leaf dry mass yield. Thus, these characteristics are suitable criteria to infer genetic distance studies in P. notatum . The selection index based on the ideotype is an auxiliary tool in the breeding process. The ideotype must be based on characteristics of interest according to the objective of the breeding program, as well as on the breeder’s prior knowledge in relation to culture.