Abstract Background Chikungunya is a mosquito-borne disease caused by the chikungunya virus (CHIKV) and can lead to neurological complications in severe cases. Objective This study examined neuroimaging patterns in chikungunya cases during two outbreaks in Brazil to identify specific patterns for diagnosis and treatment of neuro-chikungunya. Methods Eight patients with confirmed chikungunya and neurological involvement were included. Clinical examinations and MRI scans were performed, and findings were analyzed by neuroradiologists. Data on age, sex, neurological symptoms, diagnostic tests, MRI findings, and clinical outcomes were recorded. Results Patients showed different neuroimaging patterns. Six patients exhibited a "clock dial pattern" with hyperintense dotted lesions in the spinal cord periphery. One patient had thickening and enhancement of anterior nerve roots. Brain MRI revealed multiple hyperintense lesions in the white matter, particularly in the medulla oblongata, in six patients. One patient had a normal brain MRI. Conclusion The "clock dial pattern" observed in spinal cord MRI may be indicative of chikungunya-related nervous system lesions. Isolated involvement of spinal cord white matter in chikungunya can help differentiate it from other viral infections. Additionally, distinct brainstem involvement in chikungunya-associated encephalitis, particularly in the rostral region, sets it apart from other arboviral infections. Recognizing these neuroimaging patterns can contribute to early diagnosis and appropriate management of neuro-chikungunya. mosquitoborne mosquito borne CHIKV (CHIKV neurochikungunya. neurochikungunya neuro chikungunya. neuro-chikungunya included performed neuroradiologists age sex symptoms tests recorded clock pattern periphery roots oblongata chikungunyarelated related infections Additionally chikungunyaassociated associated encephalitis region

Resumo Antecedentes A chikungunya é uma doença transmitida por mosquitos causada pelo vírus chikungunya (CHIKV) e pode levar a complicações neurológicas em casos graves. Objetivo Este estudo examinou padrões de neuroimagem em casos de chikungunya durante dois surtos no Brasil para identificar padrões específicos para o diagnóstico e tratamento de neurochikungunya. Métodos Oito pacientes com chikungunya confirmada e envolvimento neurológico foram incluídos. Exames clínicos e ressonâncias magnéticas (RM) foram realizados, e os achados foram analisados por neurorradiologistas. Dados sobre idade, sexo, sintomas neurológicos, testes diagnósticos, achados de RM e desfechos clínicos foram registrados. Resultados Os pacientes apresentaram diferentes padrões de neuroimagem. Seis pacientes apresentaram um "padrão de mostrador de relógio" com lesões pontilhadas hiperintensas na periferia da medula espinhal. Um paciente apresentou espessamento e realce das raízes nervosas anteriores. A RM do cérebro revelou múltiplas lesões hiperintensas na substância branca, especialmente no bulbo em seis pacientes. Um paciente apresentou uma RM cerebral normal. Conclusão O "padrão de mostrador de relógio" observado na RM da medula espinhal pode ser indicativo de lesões do sistema nervoso relacionadas à chikungunya. O envolvimento isolado da substância branca da medula espinhal na chikungunya pode ajudar a diferenciá-la de outras infecções virais. Além disso, o envolvimento distinto do tronco cerebral na encefalite associada à chikungunya, especialmente na região rostral, a distingue de outras infecções por arbovírus. O reconhecimento desses padrões de neuroimagem pode contribuir para o diagnóstico precoce e manejo adequado da neurochikungunya. CHIKV (CHIKV graves neurochikungunya incluídos (RM realizados neurorradiologistas idade sexo neurológicos diagnósticos registrados padrão relógio anteriores normal diferenciála diferenciá la virais disso rostral arbovírus

Abstract Objective: To assess the predictive value of selected growth phenotypes for neonatal morbidity and mortality in preterm infants < 30 weeks and to compare them with INTERGROWTH-21st (IG21). Methods: Retrospective analysis of data from the Brazilian Neonatal Research Network (BNRN) database for very low birth weight (VLBW) at 20 public tertiary-care university hospitals. Outcome: the composite neonatal morbidity and mortality (CNMM) consisted of in-hospital death, oxygen use at 36 weeks, intraventricular hemorrhage grade 3 or 4, and Bell stage 2 or 3 necrotizing enterocolitis. Selected growth phenotypes: small-for-gestational-age (SGA) defined as being < 3rd (SGA3) or 10th (SGA10) percentiles of BW, and large-for-gestational-age (LGA) as being > 97th percentile of BW. Stunting as being < 3rd percentile of the length and wasting as being < 3rd percentile of BMI. Single and multiple log-binomial regression models were fitted to estimate the relative risks of CNMM, comparing them to IG21. Results: 4,072 infants were included. The adjusted relative risks of CNMM associated with selected growth phenotypes were (BNRN/IG21): 1.45 (0.92–2.31)/1.60 (1.27–2.02) for SGA; 0.90 (0.55–1.47)/1.05 (0.55–1.99) for LGA; 1.65 (1.08–2.51)/1.58 (1.28–1.96) for stunting; and 1.48 (1.02–2.17) for wasting. Agreement between the two references was variable. The growth phenotypes had good specificity (>95%) and positive predictive value (70-90%), with poor sensitivity and low negative predictive value. Conclusion: The BNRN phenotypes at birth differed markedly from the IG21 standard and showed poor accuracy in predicting adverse neonatal outcomes. Objective INTERGROWTH21st INTERGROWTHst INTERGROWTH 21st st IG . (IG21) Methods (BNRN VLBW (VLBW tertiarycare tertiary care hospitals Outcome (CNMM inhospital hospital death 4 enterocolitis smallforgestationalage small gestational age SGA (SGA rd SGA3 (SGA3 th SGA10 (SGA10 BW largeforgestationalage large LGA (LGA BMI logbinomial log binomial Results 4072 072 4,07 included BNRN/IG21 BNRNIG21 BNRNIG (BNRN/IG21) 145 1 45 1.4 0.92–2.31/1.60 092231160 0.92–2.31 /1.60 0 92 31 60 (0.92–2.31)/1.6 1.27–2.02 127202 27 02 (1.27–2.02 090 90 0.9 0.55–1.47/1.05 055147105 0.55–1.47 /1.05 55 47 05 (0.55–1.47)/1.0 0.55–1.99 055199 99 (0.55–1.99 165 65 1.6 1.08–2.51/1.58 108251158 1.08–2.51 /1.58 08 51 58 (1.08–2.51)/1.5 1.28–1.96 128196 28 96 (1.28–1.96 stunting 148 48 1.02–2.17 102217 17 (1.02–2.17 variable >95% 95 (>95% 7090%, 7090 70 90% , (70-90%) Conclusion IG2 outcomes (IG21 SGA1 (SGA1 407 07 4,0 BNRN/IG2 BNRNIG2 (BNRN/IG21 14 1. 0.92–2.31/1.6 09223116 092231 0.92–2.3 160 /1.6 9 6 (0.92–2.31)/1. 1.27–2.0 12720 (1.27–2.0 09 0. 0.55–1.47/1.0 05514710 055147 0.55–1.4 105 /1.0 5 (0.55–1.47)/1. 0.55–1.9 05519 (0.55–1.9 16 1.08–2.51/1.5 10825115 108251 1.08–2.5 158 /1.5 (1.08–2.51)/1. 1.28–1.9 12819 (1.28–1.9 1.02–2.1 10221 (1.02–2.1 >95 (>95 7090% 709 7 (70-90% (IG2 40 BNRN/IG (BNRN/IG2 0.92–2.31/1. 0922311 09223 0.92–2. /1. (0.92–2.31)/1 1.27–2. 1272 (1.27–2. 0.55–1.47/1. 0551471 05514 0.55–1. 10 (0.55–1.47)/1 0551 (0.55–1. 1.08–2.51/1. 1082511 10825 1.08–2. 15 (1.08–2.51)/1 1.28–1. 1281 (1.28–1. 1.02–2. 1022 (1.02–2. >9 (>9 (70-90 (IG (BNRN/IG 0.92–2.31/1 0922 0.92–2 /1 (0.92–2.31)/ 1.27–2 127 (1.27–2 0.55–1.47/1 0.55–1 (0.55–1.47)/ 055 (0.55–1 1.08–2.51/1 1082 1.08–2 (1.08–2.51)/ 1.28–1 128 (1.28–1 1.02–2 102 (1.02–2 (> (70-9 0.92–2.31/ 092 0.92– / (0.92–2.31) 1.27– 12 (1.27– 0.55–1.47/ 0.55– (0.55–1.47) (0.55– 1.08–2.51/ 108 1.08– (1.08–2.51) 1.28– (1.28– 1.02– (1.02– ( (70- 0.92 (0.92–2.31 1.27 (1.27 0.55 (0.55–1.47 (0.55 1.08 (1.08–2.51 1.28 (1.28 1.02 (1.02 (70 (0.92–2.3 1.2 (1.2 0.5 (0.55–1.4 (0.5 1.0 (1.08–2.5 (1.0 (7 (0.92–2. (1. (0. (1.08–2. (0.92–2 (1 (0 (1.08–2 (0.92– (1.08– (0.92 (1.08 (0.9

Abstract Objective: To verify the students’ perception about diabetes in public schools. Methods: Descriptive cross-sectional study, with students’ interview to access their diabetes knowledge (signs and symptoms, food, and diabetes self-care at school). Content analysis was used for the open question, expressed by number of occurrences, and frequencies, means and standard deviations for the other results. Results: 302 students, with 9,1±1,5 years and 54% female. For “what is diabetes” there were 91 occurrences associated with negative consequences. For 95.4% of interviewees, people with diabetes cannot eat sweets, and for 32.8% diabetes is transmitted from person to person. Only 34.8% stated that the classmate with diabetes can participate in physical/recreational activities; and 32.1% replied that he/she could not have a snack with the others. Only 29.2% of the interviewees recognized that the classmate with diabetes would need to have something with sugar to eat/drink in case he/she feel bad. Conclusions: These results reinforce the need for action of diabetes education to improve students' knowledge and perception about diabetes in the school, as well as better management for students with this condition at that environment. Objective schools Methods crosssectional cross sectional study signs symptoms food selfcare self care school. school . school) question frequencies Results 30 9115 9 1 5 9,1±1, 54 female what consequences 954 95 4 95.4 sweets 328 32 8 32.8 348 34 34.8 physicalrecreational physical recreational activities 321 32.1 heshe he she others 292 29 2 29.2 eatdrink drink bad Conclusions environment 3 911 9,1±1 95. 32. 34. 29. 9,1± 9,1 9,

Resumo Objetivo: Verificar a percepção sobre o diabetes dos alunos de escolas públicas. Métodos: Estudo descritivo transversal, com entrevista aos alunos sobre diabetes (sinais e sintomas, alimentação e questões sobre o autocuidado no ambiente escolar), com análise de conteúdo para a questão aberta, expressa em número de ocorrências, e demais resultados em frequência, média e desvio padrão. Resultados: 302 estudantes, com 9,1±1,5 anos e 54% do sexo feminino. Termos associados a consequências negativas tiveram 91 ocorrências para “o que é diabetes”. Para 95,4% dos entrevistados a pessoa com diabetes não pode comer doces, e para 32,8% o diabetes é transmitido de uma pessoa para outra. Apenas 34,8% afirmaram que o colega com diabetes pode participar de atividades físicas/recreativas, e 32,1% responderam que ele não poderia lanchar junto com os demais. Somente 29,2% dos entrevistados reconheceram que o colega com diabetes precisaria ter consigo algo com açúcar para momentos em que se sinta mal. Conclusões: Estes achados reforçam a necessidade de melhorar o conhecimento e a percepção dos estudantes sobre o diabetes no ambiente escolar, propiciando condições mais harmoniosas e seguras de manejo do diabetes nestes espaços. Objetivo públicas Métodos transversal sinais sintomas escolar , escolar) aberta frequência padrão Resultados 30 9115 9 1 5 9,1±1, 54 feminino diabetes. . diabetes” 954 95 4 95,4 doces 328 32 8 32,8 outra 348 34 34,8 físicasrecreativas físicas recreativas físicas/recreativas 321 32,1 292 29 2 29,2 mal Conclusões espaços 3 911 9,1±1 95, 32, 34, 29, 9,1± 9,1 9,

ABSTRACT Objective: To evaluate the prevalence and incidence of insufficient physical activity in Brazilian adolescents and identify the most affected subgroups during the pandemic. Methods: This study used data from the “ConVid Adolescents - Behavior Survey”, which evaluated the behavior of 9,470 Brazilian adolescents during the period of social restriction due to the COVID-19 pandemic in 2020, through a self-administered online questionnaire. Participants were invited through a virtual “snowball” sampling procedure. Information was reported on the frequency of physical activity before and during the pandemic. The exposure variables used were gender, age group, race/skin color, region of Brazil, type of school, maternal education, financial difficulties during the pandemic, and social restrictions. Logistic regression models were used. Results: Adolescents engaged in less physical activity during the pandemic, as the prevalence of insufficient physical activity increased from 71.3% in the previous period to 84.3% during the pandemic. The incidence of insufficient physical activity during the pandemic was 69.6%. The subgroups of adolescents most affected were those who self-declared as black or with dark skin color, reported financial difficulties during the pandemic, lived in the Southeast and South regions of the country, and practiced intense or complete social distancing. Conclusion: High incidences of insufficient physical activity were observed among Brazilian adolescents during the COVID-19 pandemic. It is recommended that further studies explore periods after those analyzed to identify the behavioral dynamics of adolescents upon returning to in-person activities. Objective Methods ConVid Survey, Survey , Survey” 9470 9 470 9,47 COVID19 COVID 19 COVID-1 2020 selfadministered self administered questionnaire snowball “snowball procedure gender group raceskin race color Brazil school education restrictions Results 713 71 3 71.3 843 84 84.3 696 69 6 69.6% selfdeclared declared country distancing Conclusion inperson person activities 947 47 9,4 COVID1 1 COVID- 202 7 71. 8 84. 69.6 94 4 9, 20 69. 2

RESUMO Objetivo: Avaliar a prevalência e a incidência de atividade física insuficiente em adolescentes brasileiros e identificar os subgrupos mais afetados durante a pandemia. Métodos: Este estudo utilizou dados da ConVid Adolescentes - Pesquisa de Comportamentos, que avaliou, por meio de um questionário online autopreenchido, o comportamento de 9.470 adolescentes brasileiros no período de restrição social, em virtude da pandemia da COVID-19 em 2020. Os participantes foram convidados por meio de um procedimento de amostragem em cadeia denominado “bola de neve” virtual. Foram relatadas informações sobre a frequência de atividade física antes e durante a pandemia. As variáveis de exposição utilizadas foram sexo, faixa etária, raça/cor da pele, região do Brasil, tipo de escola, escolaridade materna, dificuldades financeiras durante a pandemia e restrições sociais. Foram utilizados modelos de regressão logística. Resultados: Os adolescentes praticaram menos atividade física durante a pandemia, visto que a prevalência de atividade física insuficiente aumentou de 71,3% no período anterior para 84,3% durante a pandemia. A incidência de atividade física insuficiente durante a pandemia foi de 69,6%. Os subgrupos de adolescentes mais afetados foram os que se autodeclararam da raça/cor da pele preta, que relataram dificuldades financeiras durante a pandemia, residentes nas regiões Sudeste e Sul do país e que realizaram restrição social intensa e total. Conclusão: Altas incidências de atividade física insuficiente foram observadas entre adolescentes brasileiros durante a pandemia da COVID-19. Recomenda-se que novos estudos explorem períodos posteriores aos analisados para identificar a dinâmica comportamental dos adolescentes a partir do retorno das atividades presenciais. Objetivo Métodos Comportamentos avaliou autopreenchido 9470 9 470 9.47 COVID19 COVID 19 COVID-1 2020 bola neve virtual sexo etária raçacor raça cor Brasil escola materna sociais logística Resultados 713 71 3 71,3 843 84 84,3 696 69 6 69,6% preta total Conclusão COVID19. 19. Recomendase Recomenda presenciais 947 47 9.4 COVID1 1 COVID- 202 7 71, 8 84, 69,6 94 4 9. 20 69, 2

Abstract Objective: To verify the students’ perception about diabetes in public schools. Methods: Descriptive cross-sectional study, with students’ interview to access their diabetes knowledge (signs and symptoms, food, and diabetes self-care at school). Content analysis was used for the open question, expressed by number of occurrences, and frequencies, means and standard deviations for the other results. Results: 302 students, with 9,1±1,5 years and 54% female. For “what is diabetes” there were 91 occurrences associated with negative consequences. For 95.4% of interviewees, people with diabetes cannot eat sweets, and for 32.8% diabetes is transmitted from person to person. Only 34.8% stated that the classmate with diabetes can participate in physical/recreational activities; and 32.1% replied that he/she could not have a snack with the others. Only 29.2% of the interviewees recognized that the classmate with diabetes would need to have something with sugar to eat/drink in case he/she feel bad. Conclusions: These results reinforce the need for action of diabetes education to improve students' knowledge and perception about diabetes in the school, as well as better management for students with this condition at that environment.

Resumo Objetivo: Verificar a percepção sobre o diabetes dos alunos de escolas públicas. Métodos: Estudo descritivo transversal, com entrevista aos alunos sobre diabetes (sinais e sintomas, alimentação e questões sobre o autocuidado no ambiente escolar), com análise de conteúdo para a questão aberta, expressa em número de ocorrências, e demais resultados em frequência, média e desvio padrão. Resultados: 302 estudantes, com 9,1±1,5 anos e 54% do sexo feminino. Termos associados a consequências negativas tiveram 91 ocorrências para “o que é diabetes”. Para 95,4% dos entrevistados a pessoa com diabetes não pode comer doces, e para 32,8% o diabetes é transmitido de uma pessoa para outra. Apenas 34,8% afirmaram que o colega com diabetes pode participar de atividades físicas/recreativas, e 32,1% responderam que ele não poderia lanchar junto com os demais. Somente 29,2% dos entrevistados reconheceram que o colega com diabetes precisaria ter consigo algo com açúcar para momentos em que se sinta mal. Conclusões: Estes achados reforçam a necessidade de melhorar o conhecimento e a percepção dos estudantes sobre o diabetes no ambiente escolar, propiciando condições mais harmoniosas e seguras de manejo do diabetes nestes espaços.

RESUMO Objetivo: Avaliar a prevalência e a incidência de atividade física insuficiente em adolescentes brasileiros e identificar os subgrupos mais afetados durante a pandemia. Métodos: Este estudo utilizou dados da ConVid Adolescentes - Pesquisa de Comportamentos, que avaliou, por meio de um questionário online autopreenchido, o comportamento de 9.470 adolescentes brasileiros no período de restrição social, em virtude da pandemia da COVID-19 em 2020. Os participantes foram convidados por meio de um procedimento de amostragem em cadeia denominado “bola de neve” virtual. Foram relatadas informações sobre a frequência de atividade física antes e durante a pandemia. As variáveis de exposição utilizadas foram sexo, faixa etária, raça/cor da pele, região do Brasil, tipo de escola, escolaridade materna, dificuldades financeiras durante a pandemia e restrições sociais. Foram utilizados modelos de regressão logística. Resultados: Os adolescentes praticaram menos atividade física durante a pandemia, visto que a prevalência de atividade física insuficiente aumentou de 71,3% no período anterior para 84,3% durante a pandemia. A incidência de atividade física insuficiente durante a pandemia foi de 69,6%. Os subgrupos de adolescentes mais afetados foram os que se autodeclararam da raça/cor da pele preta, que relataram dificuldades financeiras durante a pandemia, residentes nas regiões Sudeste e Sul do país e que realizaram restrição social intensa e total. Conclusão: Altas incidências de atividade física insuficiente foram observadas entre adolescentes brasileiros durante a pandemia da COVID-19. Recomenda-se que novos estudos explorem períodos posteriores aos analisados para identificar a dinâmica comportamental dos adolescentes a partir do retorno das atividades presenciais.

ABSTRACT Objective: To evaluate the prevalence and incidence of insufficient physical activity in Brazilian adolescents and identify the most affected subgroups during the pandemic. Methods: This study used data from the “ConVid Adolescents - Behavior Survey”, which evaluated the behavior of 9,470 Brazilian adolescents during the period of social restriction due to the COVID-19 pandemic in 2020, through a self-administered online questionnaire. Participants were invited through a virtual “snowball” sampling procedure. Information was reported on the frequency of physical activity before and during the pandemic. The exposure variables used were gender, age group, race/skin color, region of Brazil, type of school, maternal education, financial difficulties during the pandemic, and social restrictions. Logistic regression models were used. Results: Adolescents engaged in less physical activity during the pandemic, as the prevalence of insufficient physical activity increased from 71.3% in the previous period to 84.3% during the pandemic. The incidence of insufficient physical activity during the pandemic was 69.6%. The subgroups of adolescents most affected were those who self-declared as black or with dark skin color, reported financial difficulties during the pandemic, lived in the Southeast and South regions of the country, and practiced intense or complete social distancing. Conclusion: High incidences of insufficient physical activity were observed among Brazilian adolescents during the COVID-19 pandemic. It is recommended that further studies explore periods after those analyzed to identify the behavioral dynamics of adolescents upon returning to in-person activities.

Resumo Introdução O brincar é intrinsecamente relacionado ao desenvolvimento infantil. Através da avaliação do brincar, profissionais de saúde, incluindo terapeutas ocupacionais, podem obter informações sobre o desenvolvimento global da criança. A Escala Lúdica Pré-escolar de Knox – revisada (ELPKr) é um instrumento padronizado capaz de avaliar eficazmente as habilidades presentes no brincar de pré-escolares. Objetivo Examinar a produção científica nacional e internacional sobre a utilização da ELPKr. Método Trata-se de uma revisão de escopo da literatura, utilizando as seguintes bases de dados para busca: Scopus, Lilacs, BDENF, Embase, Cochrane, Medline, PubMed, CINAHL, Web of Science, PsycINFO, Dedalus, BDTD, PROQUEST, além de Scielo, Google Scholar e Portal Capes de Periódicos. A estratégia de busca incluiu os descritores “Escala Lúdica Pré-escolar de Knox” OR “Knox Preschool Play Scale”. Resultados Foram identificados 51 estudos publicados entre 1983 e 2019, envolvendo 1.816 crianças. Além disso, foi constatado uso associado de mais 36 instrumentos de avaliação, sendo possível indicar potencialidades e fragilidades da escala. A ELPKr é um instrumento útil e confiável tanto na prática clínica como na pesquisa. No entanto, a não existência de um manual ou orientações sobre sua forma de aplicação foi indicada como uma fragilidade do instrumento. Conclusão Esta revisão de escopo analisou qualitativamente a literatura nacional e estrangeira a respeito da ELPK, identificando lacunas nas produções científicas e gerando um amplo panorama de dados sobre a utilização, difusão e disponibilidade desse instrumento, além de apresentar um delineamento dos estudos, suas metodologias e objetivos.

Abstract Introduction Playing is intrinsically related to child development. Through play assessment, health professionals, including occupational therapists, can obtain information about child development. The Knox Preschool Play Scale - revised (KPPS-r) is a standardized instrument that enables the effective assessment of the skills existing in preschoolers’ play behavior. Objective To examine the national and international scientific production on the KPPS-r. Method This is a scoping review of the literature utilizing the following research databases: Scopus, Lilacs, BDENF, Embase, Cochrane, Medline, PubMed, CINAHL, Web of Science, PsycINFO, Dedalus, BDTD, and PROQUEST; also Scielo, Google Scholar, and Capes Articles Website. The search strategy included the descriptors “Escala Lúdica Pré-escolar de Knox” OR “Knox Preschool Play Scale”. Results 51 studies published between 1983 and 2019 involving 1816 children were identified. The associated use of over 36 assessment instruments was identified, enabling knowledge about the strengths and weaknesses of the scale. The KPPSr is a valuable and reliable instrument both in clinical practice and in research. However, the lack of a manual or guidelines for its application is a weakness of this instrument. Conclusion This scoping review qualitatively analyzed the national and international literature addressing the KPPS, identifying gaps in scientific production and generating a broad panorama of data on the use, dissemination, and availability of this instrument, in addition to presenting an outline of the studies, their methodologies, and objectives.

Abstract Background: Patients using immunosuppressive drugs may have unfavorable results after infections. However, there is a lack of information regarding COVID 19 in these patients, especially in patients with rheumatoid arthritis (RA). Therefore, the aim of this study was to evaluate the risk factors associated with COVID 19 hospitalizations in patients with RA. Methods: This multicenter, prospective cohort study is within the ReumaCoV Brazil registry and included 489 patients with RA. In this context, 269 patients who tested positive for COVID 19 were compared to 220 patients who tested negative for COVID 19 (control group). All patient data were collected from the Research Electronic Data Capture database. Results: The participants were predominantly female (90.6%) with a mean age of 53 ±12 years. Of the patients with COVID 19, 54 (20.1%) required hospitalization. After multiple adjustments, the final regression model showed that heart disease (OR =4.61, 95% CI 1.06–20.02. P < 0.001) and current use of glucocorticoids (OR =20.66, 95% CI 3.09–138. P < 0.002) were the risk factors associated with hospitalization. In addition, anosmia was associated with a lower chance of hospitalization (OR =0.26; 95% CI 0.10–0.67, P < 0.005). Conclusion: Our results demonstrated that heart disease and the use of glucocorticoids were associated with a higher number of hospital admissions for COVID 19 in patients with RA. Trial registration: Brazilian Registry of Clinical Trials RBR 33YTQC.

RESUMO: Este estudo tem como objetivo descrever um novo método de detecção de uma reação em cadeia da polimerase quantitativa em tempo real (qPCR) visando o gene da proteína da membrana externa de 28 kDa (p28), bem como comparar este método com um PCR convencional (cPCR), que visa o mesmo gene, a fim de avaliar o desempenho da técnica desenhada neste estudo na detecção de Ehrlichia canis (E. canis). As concentrações ideais de oligonucleotídeos foram alcançadas e a sensibilidade analítica e a especificidade do qPCR foram determinadas. Um total de 218 amostras de sangue total de cães foram coletadas convencionalmente para este estudo. O DNA foi extraído de cada amostra. Posteriormente, as amostras foram testadas por um cPCR estabelecido e o novo qPCR para comparar os desempenhos entre cada técnica. A curva padrão revelou 100% de eficiência e coeficiente de determinação (R2) equivalente a 99,8%. Dentre as amostras examinadas por qPCR, 24,31% foram consideradas positivas, percentual significativamente maior do que as detectadas por cPCR (15,13%). A técnica qPCR atingiu uma sensibilidade maior do que a cPCR na detecção de E. canis. A qPCR padronizada neste estudo é um método eficiente para a confirmação do diagnóstico de erliquiose monocítica canina (EMC) e pode fornecer o monitoramento de níveis de parasitemia ao longo do tratamento da doença.

ABSTRACT: This study aims to describe a new detection method of a quantitative real-time polymerase chain reaction (qPCR) targeting the 28 kDa outer membrane protein gene (p28) as well as to compare this method with a conventional PCR (cPCR), which targets the same gene, in order to evaluate the performance of the technique designed in this study in detecting Ehrlichia canis (E. canis). Optimum oligonucleotides concentrations were reached, and the analytical sensitivity and specificity of the qPCR were performed. A total of 218 dogs’ whole blood samples were conventionally collected for this study. The DNA was extracted from each sample. Subsequently, the samples were tested by an established cPCR and the new qPCR to compare each technique’s performances. This new qPCR method for the molecular detection of E. canis presented a detection limit of ten copies of the fragment and was considered specific for E. canis according to analytical specificity analyses performed in vitro and in silico. The standard curve revealed 100% efficiency and a coefficient of determination (R2) equivalent to 99.8%. Among the samples examined by qPCR, 24.31% were considered positive, significantly greater than those detected by cPCR (15.13%). The qPCR technique reached a higher sensitivity than the cPCR when targeting the p28 gene in detecting E. canis. The qPCR standardized in this study is an efficient method for confirming canine monocytic ehrlichiosis (CME) diagnosis and might provide the parasitemia monitoring during the disease treatment.

Abstract Background: In Brazil, socioeconomic differences in the incidence of rheumatoid arthritis (RA) have been demonstrated, which are important in the formulation of hypotheses regarding the association between environmental factors, lifestyle and the risk of disease development. This study examines how the socioeconomic condition of the patient with RA in Brazil, assessed according to social class, educational level, employment situation and use of caregivers, affects the times between the beginning of symptoms and diagnosis and the beginning of the use of disease-modifying antirheumatic drugs, as well as the presence of erosive disease and functional status. Methods: This work is part of a multicentric study called REAL - Rheumatoid Arthritis in Real Life in Brazil, which is a prospective observational cohort study. Results: As described in the REAL study, we included a total of 1115 patients. It was noted that patients with an educational classification of up to second grade incomplete presented with erosion percentages above those with a higher grade complete. Patients with caregivers presented a higher percentage of erosion than patients without caregivers. We verified that patients from economic classes above B2 presented fewer occurrences of erosion than those from classes C2, D-E. We also analyzed the average time differences from the beginning of symptoms and diagnosis and the beginning of treatment, according to academic level, erosion and economic classification. Patients with first grade complete showed an HAQ-DI averages higher than those with second grade complete. The patients who had employment showed lower HAQ-DI averages than patients who were not employed. The patients with erosion showed an HAQ-DI value higher than those without erosion. Patients with caregivers showed an HAQ-DI average higher than that of without caregivers. Conclusion: This study showed that the therapeutic window of RA is not being reached, and therefore we should have a policy to expand and ensure access to public health for all patients, especially those with lower levels of education and income. Trial registration: This study was approved by the National Commission of Ethics in Research.

Abstract Glaze application on monolithic zirconia (Y-TZP) can be a practical approach to improve the mechanical properties of this material. Objective Our study evaluated the effect of glazing side and mechanical cycling on the biaxial flexure strength (BFS) of a Y-TZP. Methodology Eighty sintered Y-TZP discs (Ø:12 mm; thickness: 1.2 mm - ISO 6872) were produced and randomly assigned into eight groups (n=10), according to the factors “glazing side” (control – no glazing; GT – glaze on tensile side; GC – glaze on compression side; GTC – glaze on both sides) and “mechanical aging” (non-aged and aged, A – mechanical cycling: 1.2×106, 84 N, 3 Hz, under water at 37°C). Specimens were subjected to BFS test (1 mm/min; 1,000 Kgf load cell) and fractured surfaces were analyzed by stereomicroscopy and SEM. Hsueh’s rigorous solutions were used to estimate the stress at failure of glazed specimens. Two-way ANOVA, Tukey’s test (5%), and Weibull analysis were performed. Results The “glazing side”, “mechanical aging” and the interaction of the factors were significant (p<0.05). Groups GC (1157.9±146.9 MPa), GT (1156.1±195.3 MPa), GTC (986.0±187.4 MPa) and GTC-A (1131.9±128.9 MPa) presented higher BFS than control groups (Tukey, 5%). Hsueh’s rigorous solutions showed that the maximum tensile stress was presented in the bottom of zirconia layer, at the zirconia/glaze interface. Weibull characteristic strength (σo) of the GC was higher than all groups (p<0.05), except to GT, GTC-A and GTC, which were similar among them. The fractography showed initiation of failures from zirconia the tensile side regardless of the side of glaze application and fatigue. Conclusion Glazing zirconia applied on both tensile and compression sides improves the flexural strength of Y-TZP, regardless the mechanical aging.

ABSTRACT Introduction Transplantation is the gold standard approach in the treatment of heart failure, resulting in longer survival and quality of life. However, high demand and donor shortage lead to long queues in which patients experience ambiguous feelings of hope and frustration between life and death. Objective To understand the experience in the heart transplant waiting list from the perspective of patients and their families. Method Descriptive and exploratory research with a qualitative approach, conducted with 12 participants: six patients who were waiting for heart transplantation and six family members. They answered a semi-structured interview script, which was evaluated by textual analysis using the Iramuteq software (Interface for R for Les Multesanalysis de Textes et de Questionnaires). Results It was evident that the discovery of the diagnosis and the need for transplantation was permeated by feelings of sadness and fear, requiring reorganization of the family structure and use of different coping strategies. The difficulties experienced in waiting for an organ include the emergence of clinical complications that can take the life of the patient or prevent surgery, and the constant refusal of possible donors on the part of the family. Conclusion The waiting list for heart transplantation is a moment of “race against time for life”, permeated by anxiety, hope and frustration, in which family support is essential. It is also essential to invest in organ donation campaigns and training professionals to make a correct approach to families when it comes to talk about potential donors, embracing their grief and clarifying their doubts.

RESUMO Introdução O transplante é a abordagem padrão-ouro no tratamento da insuficiência cardíaca, resultando em maior sobrevida e qualidade de vida. Entretanto, a alta demanda e escassez de doadores geram longas filas, nas quais pacientes vivenciam sentimentos ambíguos de esperança e frustação, em meio a díade vida e morte. Objetivo Compreender a vivência na fila de espera do transplante cardíaco a partir da perspectiva do paciente e sua família. Método Pesquisa descritiva e exploratória, de abordagem qualitativa, realizada com 12 participantes: seis pacientes que estão em fila de espera para transplante cardíaco e seis familiares. Eles responderam um roteiro de entrevista semiestruturado, que foi avaliado por meio de análise textual no software Iramuteq (Interface de R pour les Analyses Multimensionnelles de Textes et de Questionnaires). Resultados Evidenciou-se que a descoberta do diagnóstico e da necessidade de transplante é permeada por sentimentos de tristeza e medo, que demandam reorganização da estrutura familiar e uso de diferentes estratégias de enfrentamento. Entre as dificuldades vivenciadas na espera por um órgão contemplam-se o surgimento de intercorrências clínicas, que podem lhe tirar a vida ou impossibilitar a cirurgia, e a constante recusa da família de possíveis doadores. Conclusão A fila de espera para transplante cardíaco configura-se como um momento de “corrida contra o tempo pela vida”, permeada por ansiedade, esperança e frustração, na qual o apoio familiar é fundamental. Faz-se fundamental também o investimento em campanhas de doação de órgãos e capacitação de profissionais para fazer uma abordagem correta aos familiares de possíveis doadores, acolhendo o seu luto e esclarecendo suas dúvidas.

RESUMEN Introducción El trasplante es el enfoque patrón-oro en el tratamiento de la insuficiencia cardíaca, resultando en una mayor sobrevida y calidad de vida. Sin embargo, la alta demanda y escasez de donantes generan largas filas, en las que los pacientes experimentan sentimientos ambiguos de esperanza y frustación, en medio de la vida y la muerte. Objetivo Comprender la vivencia en la cola de espera del trasplante cardíaco desde la perspectiva del paciente y su familia. Método Investigación descriptiva y exploratoria, de abordaje cualitativo, realizada con 12 participantes: 6 pacientes que están en fila de espera para trasplante cardíaco y 6 familiares. En el programa Iramuteq (Interfaz de R para los Analyses Multimensionnelles de Textes et de Questionnaires), respondieron un guión de entrevista semiestructurado, que fue evaluado por medio de análisis textual en el software Iramuteq. Resultados Se evidenció que el descubrimiento del diagnóstico y de la necesidad de trasplante está impregnado por sentimientos de tristeza y miedo, que demandan reorganización de la estructura familiar y uso de diferentes estrategias de enfrentamiento. Entre las dificultades vivenciadas en la espera por un órgano se contempla el surgimiento de intercurrencias clínicas, que pueden quitarle la vida o imposibilitar la cirugía, y el constante rechazo de la familia de posibles donantes. Conclusión La cola de espera para trasplante cardíaco se configura como un momento de "carrera contra el tiempo por la vida", impregnada por ansiedad, esperanza y frustración, en la que el apoyo familiar es fundamental. Se hace fundamental también la inversión en campañas de donación de órganos y capacitación de profesionales para hacer un abordaje correcto a los familiares de posibles donantes, acogiendo su duelo y aclarando sus dudas.

Resumo Políticos e profissionais de meio ambiente têm apontado para a ausência de conhecimento científico para embasamento da legislação que dispõe sobre o planejamento urbano. Assim, o intuito desse ensaio é disponibilizar conhecimento ecológico para auxiliar políticos e profissionais de meio ambiente na tomada de decisões relacionadas ao zoneamento urbano do Brasil. Como ciência, a ecologia disponibiliza o conhecimento necessário para auxiliar a criação de diretrizes e normas para o zoneamento urbano. Especificamente, a ecologia de paisagem aponta diferentes métodos de trabalho, que possuem parâmetros de qualificação e quantificação do ambiente urbano. Esses parâmetros podem ser utilizados como critérios para o planejamento de cidades sustentáveis. Já a ecologia urbana tem demonstrado a importância da conservação de áreas verdes e azuis (corpos de água) nas cidades, além da necessidade da formação de equipes transdisciplinares para uma gestão urbana e elaboração de políticas públicas que visem a estratégia de zoneamento sustentável. De modo geral, a ciência da ecologia demonstra contribuições que auxiliam na criação de diretrizes e normas para o zoneamento urbano brasileiro. As sugestões fornecidas através desse ensaio constituem um ponto de partida para a formulação de melhorias nas leis destinadas a desenvolver e fortalecer a legislação relativa ao zoneamento urbano brasileiro.

Abstract Politicians and environmental professionals have pointed to the lack of scientific knowledge to support legislation for planning urban centers. Thus, this essay aims to provide ecological knowledge to assist politicians and environmental professionals in decision making regarding the Brazilian urban zoning. As a science, ecology provides the necessary knowledge to assist the creation of guidelines and standards for urban zoning. Specifically, landscape ecology shows different methods of work, which have as parameters the qualification and quantification of urban environments. These parameters can be used as criteria for planning sustainable cities. The field of urban ecology has demonstrated the importance of conserving green and blue areas (water bodies) in cities, as well as the need for the formation of multidisciplinary teams for urban management and elaboration of public policies pointed to zoning sustainability strategies. Generally, ecology show contributions that assist in the creation of guidelines and norms for Brazilian urban zoning. The suggestions provided through this essay are a starting point for improving the formulation of laws designed to develop and strengthen the legislation on Brazilian urban zoning.

RESUMO CONTEXTO: A doença hepática gordura não-alcoólica caracteriza-se pela deposição de lipídios no parênquima hepático, excedendo 5% do peso do fígado na ausência de outras afecções como hepatites virais, alcoólicas ou doenças metabólicas. A doença hepática gordura não-alcoólica tem sido observada como a forma mais comum de doença hepática crônica em diversos países. Além das complicações hepáticas, estudos recentes têm demonstrado a relação entre a presença de gordura hepática e a sarcopenia. OBJETIVO: Determinar a associação entre a sarcopenia e a gravidade da esteatose hepática não-alcoólica diagnosticada pela ultrassonografia abdominal. MÉTODOS: Estudo clínico e transversal com amostra de pacientes de ambos os sexos, de 18 a 70 anos de idade, diagnosticados como portadores ou não de esteatose hepática não-alcoólica pela ultrassonografia e submetidos à avaliação da força muscular dos membros superiores e inferiores. Os dados foram inseridos no programa estatístico SPSS 22.0, analisados através do teste ANCOVA e pós-teste de Bonferroni, sendo considerado significante P<0,05. RESULTADOS: Foram avaliados pela ultrassonografia abdominal 102 pacientes e destes, 57,8% apresentaram algum grau de esteatose hepática não-alcoólica. A presença e os graus da infiltração gordurosa no fígado tiveram associação estatisticamente significativa com o índice sarcopênico, determinado pela razão entre força muscular dos membros superiores e inferiores e o IMC (P=0,009 e pós-teste P=0,028 MMSS; P=0,006 e pós-teste P=0,013 MMII). CONCLUSÃO: Observou-se associação entre o índice sarcopênico e a presença de esteatose hepática não-alcoólica, com relação inversamente proporcional entre esse índice e a gravidade da infiltração gordurosa, reforçando a interação do eixo metabólico entre fígado, tecido adiposo e músculo.

ABSTRACT BACKGROUND: Non-alcoholic fatty liver disease is characterized by deposition of lipids in the hepatic parenchyma exceeding 5% of liver weight in the absence of other conditions, such as viral or alcoholic hepatitis and metabolic disease. Non-alcoholic fatty liver disease is the most common form of chronic liver disease in several countries. In addition to liver complications, recent studies have shown a relation between liver fat and sarcopenia. OBJECTIVE: Determine the association between sarcopenia and the severity of non-alcoholic hepatic steatosis diagnosed by abdominal ultrasonography. METHODS: A clinical, cross-sectional study was conducted with a sample of male and female adults (18 to 70 years of age) submitted to ultrasonography for the investigation of non-alcoholic hepatic steatosis. Evaluations were also performed for the determination of upper and lower limb muscle strength. Data analysis was performed with the aid of the SPSS 22.0 program and involved ANCOVA and the Bonferroni post hoc test, with P-value <0.05 considered indicative of statistical significance. RESULTS: One hundred two patients were submitted to abdominal ultrasonography, 57.8% of whom presented some degree of non-alcoholic hepatic steatosis. The presence and degree of fatty liver infiltration were significantly associated with the sarcopenic index, determined by the ratio between upper and lower limb strength and BMI (P=0.009 and post-test P=0.028 for upper limbs; P=0.006 and post-test P=0.013 for lower limbs). CONCLUSION: In the present study, an association was found between the sarcopenic index and non-alcoholic hepatic steatosis, with an inversely proportional relation between this index and the severity of fatty infiltration. This finding offers further evidence of the metabolic interaction of the liver, adipose tissue and muscle.

RESUMO A ferrugem asiática da soja (FAS), causada pelo fungo Phakopsora pachyrhizi, é uma das doenças mais severas na cultura. Dentre as estratégias para seu manejo propõe-se a utilização de genótipos com resistência horizontal ou parcial, ainda restritos no mercado, mas promissores pela durabilidade da resistência. O objetivo deste trabalho foi avaliar a resistência de diferentes genótipos de soja à ferrugem asiática para futura utilização em programas de melhoramento. Na safra 2015/2016, foram avaliados 11 genótipos de soja provenientes do LAGER-UFU e a cultivar Desafio 8473 (padrão de suscetibilidade) frente à infecção artificial e natural de P. pachyrhizi no campo. Com os dados de severidade da FAS, calculou-se a área abaixo da curva de progresso da doença (AACPD). O percentual de área foliar afetada e os valores da AACPD dos genótipos avaliados oscilaram de 1,56 a 80,75 e de 380,62 a 975,62, respectivamente. Os genótipos L-210, L-166, L-268, L-203, L-279, L-224, L-216 e L-218 apresentam menores valores de AACPD, indicando resistência parcial, enquanto os genótipos L-144, L-104, L-266 e a cv. Desafio 8473 apresentaram maiores valores de AACPD, portanto, com menor resistência parcial ou suscetíveis. Ainda assim, os genótipos L-144, L-104 e L-266 apresentaram coeficientes de progresso da severidade da doença de 1,8 a 2,0 vezes menores comparativamente à cv. Desafio 8473. Conclui-se que os genótipos do Programa de Melhoramento Genético da UFU (LAGER-UFU), apresentam potencial de utilização no desenvolvimento de cultivares de soja resistentes à P. pachyrhizi.

ABSTRACT Asian soybean rust (ASR), caused by the fungus Phakopsora pachyrhizi, is one of the most severe diseases that affect the crop. Among management strategies is the use of genotypes with horizontal or partial resistance, which are still restricted in the market but promising due to their resistance durability. The objective of this study was to evaluate the resistance of different genotypes to Asian soybean rust for future use in breeding programs. In the 2015/2016 crop season, 11 soybean genotypes from LAGER-UFU and cv. Desafio8473 (susceptibility pattern) were evaluated for artificial and natural infection by P. pachyrhizi in the field. ASR severity data were used to calculate the area under the disease progress curve (AUDPC). The percentage of affected leaf area and the AUDPC values of the evaluated genotypes ranged from 1.56 to 80.75% and from 380.62 to 975.62, respectively. The genotypes L-210, L-166, L-268, L-203, L-279, L-224, L-216 and L-218 have lower AUDPC values, indicating partial resistance, while the genotypes L-144, L-104, L-266 and cv. Desafio 8473 showed higher AUDPC values and therefore lower partial resistance or susceptibility. Even so, the genotypes L-144, L-104 and L-266 showed coefficients of disease severity progress 1.8 to 2.0-fold lower than those of cv. Desafio 8473. In conclusion, the genotypes from UFU Breeding Program (LAGER-UFU) showed potential to be used in the development of soybean cultivars resistant to P. pachyrhizi.