O objetivo deste estudo foi analisar a correlação entre níveis séricos de triglicérides (TG) em pacientes obesos e não obesos em estado pós-prandial simulado. Os grupos apresentavam níveis de TG em jejum < 150 mg/dL. Após 12 h de jejum, os indivíduos ingeriram uma sobrecarga lipídica e o sangue foi coletado. A variação entre os picos dos níveis de TG em jejum e em estado pós-prandial foi analisada. Os picos pós-prandiais de TG ocorreram 4 h depois da sobrecarga lipídica nos 2 grupos. Após a refeição, grande parte dos indivíduos não obesos permaneceu dentro da normalidade em relação aos níveis de TG, mas os valores para os obesos permaneceram elevados. Houve uma correlação significativa entre Índice de Massa Corporal (IMC) e TG até 2 h após a refeição, mas os dados não mostraram correlação após 3 h. De acordo com a curva ROC (receiver-operating characteristics), os valores de TG pós-prandiais não são um bom indicador de obesidade (baseado no IMC), mas são bons indicadores de não obesidade entre os indivíduos. Este trabalho reforça a importância de se medir níveis pós-prandiais de TG em obesos e não obesos, já que alguns pacientes não obesos provavelmente apresentavam um metabolismo alterado de gordura, indicando que este tipo de exame poderia ser um indicador de risco metabólico.

The aim of this study was to analyze the correlation between triglyceride (TG) serum levels in obese and non-obese patients in a simulated postprandial state. Both groups showed TG levels < 150 mg/dL when fasting. After 12 h fasting, the subjects ingested a lipid overload diet and blood samples were collected. The variation between fasting and the postprandial TG peak levels were analyzed. The peak of postprandial TG levels occurred 4 h after the lipid overload in both groups. When the subjects were not fasting, the majority of non-obese subjects remained within the range of normal TG values, but the values for the obese group remained elevated. There was a significant correlation between Body Mass Index (BMI) and TG at each time point until 2 h after the meal, but the data did not show a correlation after 3 h. According to the receiver-operating characteristics (ROC) curve, postprandial TG values were not a good predictor of obesity (based on BMI), but they were a predictor of non-obesity. This study reinforces the importance of measuring non-fasting TG levels in obese and non-obese subjects, because some non-obese patients probably had altered fat metabolism, indicating that this examination could be an indicator of metabolic risk.

Maturity Onset Diabetes of the Young (MODY) presents monogenic inheritance and mutation factors which have already been identified in six different genes. Given the wide molecular variation present in the hepatocyte nuclear factor-1α gene (HNF1α) MODY3, the aimof this study was to amplify and sequence the coding regions of this gene in seven patients from the Campos Gerais region, Paraná State, Brazil, presenting clinical MODY3 features. Besides the synonymous variations, A15A, L17L, Q141Q, G288G and T515T, two missense mutations, I27L and A98V, were also detected. Clinical and laboratory data obtained from patients were compared with the molecular findings, including the I27L polymorphism that was revealed in some overweight/obese diabetic patients of this study, this corroborating with the literature. We found certain DNA variations that could explain the hyperglycemic phenotype of the patients.