Abstract Objective: To investigate the incidence, clinical and genetic characteristics of pediatric lymphoma patients of China with inborn errors of immunity (IEI)-related gene mutations, which have not been fully studied. Method: From Jan. 2020 to Mar. 2023, IEI-related genetic mutations were retrospectively explored in 108 children with lymphomas admitted to Beijing Children’s Hospital by NGS. Genetic rule and clinical characteristics as well as treatment outcomes were compared between patients with or without IEI-related gene mutations. Results: A total of 17 patients (15.7 %) harbored IEI-associated mutations, including 4 cases with X-linked lymphoproliferative syndrome (XLP), 3 cases had mutations in tumor necrosis factor receptor superfamily 13B (TNFRSF13B), 2 cases with Activated p110 syndrome (APDS). Patients with IEI all had alteration of immunocompetence with decreased levels of immunoglobulin and lymphocyte subsets. Recurrent infection existed in 41.2 % of patients. The 18-month event-free survival (EFS) and the overall response rate (ORR) of patients with IEI are significantly lower than those without IEI (33.86% vs. 73.26 %, p = 0.011; 52.94% vs. 87.91 %, p = 0.002, respectively). In addition, patients with IEI had a higher progression disease (PD) rate of 23.5 % than those without IEI of 4.4% (p = 0.006). Conclusion: The present study demonstrated that IEI-associated lymphomas were much more common than originally appreciated in pediatric lymphomas, and those were insensitive to treatment and more likely to progress or relapse. The genomic analysis and a thorough review of the medical history of IEI can be used to distinguish them from pediatric lymphomas without IEI, which are beneficial for the early diagnosis and direct intervention. Objective incidence IEIrelated related studied Method Jan 202 Mar 2023 10 Childrens Children s NGS Results 1 15.7 157 15 7 (15. IEIassociated associated Xlinked X linked XLP, XLP , (XLP) B TNFRSF13B, TNFRSF13B TNFRSFB TNFRSF (TNFRSF13B) p11 APDS. APDS . (APDS) subsets 412 41 41. 18month month 18 eventfree event free EFS (EFS ORR (ORR 33.86% 3386 33 86 (33.86 vs 7326 73 26 73.2 0.011 0011 0 011 5294 52 94 52.94 8791 87 91 87.9 0002 002 0.002 respectively. respectively respectively) addition PD (PD 235 23 5 23. 44 4.4 0.006. 0006 0.006 006 0.006) Conclusion relapse intervention 20 15. (15 (XLP (TNFRSF13B p1 (APDS 33.86 338 8 (33.8 732 73. 0.01 001 01 529 9 52.9 879 87. 000 00 0.00 4. (1 33.8 (33. 0.0 52. ( 33. (33 0. (3

RESUMEN La inundación de CO2 para la recuperación de petróleo es un proceso dinámico que requiere más investigación sobre las características de cambio de la interfaz petróleo-gas, los procesos de transferencia de masa interfacial y la variación de la composición petróleo-gas durante el desplazamiento tanto inmiscible como miscible. Comprender estos factores es crucial para comprender mejor su impacto en la recuperación de petróleo mejorada con CO2 (EOR). Esta investigación adopta un aparato experimental de inundación visual miscible con CO2 desarrollado conjuntamente para estudiar las características dinámicas horizontales del CO2 y el petróleo crudo bajo diferentes presiones y caudales en un tubo visual delgado. A 10 MPa, los resultados de la estratificación del CO2 y del petróleo crudo indican que el experimento es una inundación inmiscible. El ángulo de contacto (7,9°) entre las dos fases de CO2 y el petróleo crudo a un caudal de 15 cm/min es mayor que el (5,2°) a 1,5 cm/ min, y la escala de grises del CO2 aumenta a 100 cm/min. La cantidad, el contenido individual y la forma de los componentes de hidrocarburos ligeros y medios condensados en la pared interior del tubo varían con diferentes caudales. A 15 MPa, la aparición del intervalo de transición de CO2 y petróleo crudo demuestra que el experimento es una inundación miscible. A diferentes caudales, el ángulo de inclinación y la distribución de las franjas negras son diferentes. Todo el intervalo de transición se divide en 6 intervalos y el intervalo de transición se alarga al aumentar la velocidad del fluido. Los experimentos demuestran visualmente la aparición de la fase miscible e identifican la presión experimental y el caudal de fluido como factores clave que influyen en la miscibilidad del CO2 y el petróleo crudo.

ABSTRACT CO2 flooding for oil recovery is a dynamic process that requires further investigation of oil-gas interface change characteristics, interfacial mass transfer processes, and oil-gas composition variation during both immiscible and miscible displacement. Understanding these factors is crucial for better comprehending their impact on CO2-enhanced oil recovery (EOR). This research used a jointly developed CO2 miscible visual flooding experimental apparatus to study the horizontal dynamic characteristics of CO2 and crude oil under different pressures and flow rates in visual slim tube. At 10 MPa, the stratification results of CO2 and crude oil indicate that the experiment is immiscible flooding. The contact angle (7.9°) between the two phases of CO2 and crude oil at the flow rate of 15 cm/min is larger than that (5.2°) at 1.5 cm/min, and the grey scale of CO2 increases at 100 cm/min. The quantity, individual content, and shape of the light and medium hydrocarbon components condensed on the inner wall of the tube vary with different flow rates. At 15 MPa, the appearance of the CO2 and crude oil transition interval proves that the experiment is miscible flooding. At different flow rates, the inclination angle and distribution of black stripes vary. The whole transition interval is divided into 6 intervals, and the transition interval lengthens with increasing fluid velocity. The experiments visually demonstrate the occurrence of the miscible phase, and identify experimental pressure and fluid flow rate as key factors influencing the miscibility of CO2 and crude oil.

SUMMARY: The objective of this study was to observe the clinical efficacy of apatinib (AP) combined with 131I in the treatment of radioiodine-refractory differentiated thyroid cancer (RAIR-DTC) and the prognostic significance of MIP-1α after treatment, and to provide reference and guidance for future treatment and disease assessment of RAIR-DTC. One hundred and six patients with RAIR- DTC admitted to our hospital from January 2019 to October 2020 were selected for the study. All the patients were treated with TC surgery with 131I at our hospital, and 58 of them were subsequently transferred to AP treatment, which was considered as the research group; the other 48 patients were transferred to thyroid stimulating hormone (TSH) suppression treatment, which was considered as the control group. The clinical efficacy of the research group was better than that of the control group (P < 0.05), while no difference was seen in the comparison of the incidence of adverse effects and thyroid function (P > 0.05). After treatment, Tg, TL, maximum diameter of C/B lymph nodes, number of lymph nodes and number of calcified spots were lower in the research group than in the control group (P < 0.05). ROC analysis revealed that the predictive sensitivity of MIP-1α for prognosis of 3-year RAIR-DTC death in the research group of patients was 84.63 % and the specificity was 72.16 %. AP combined with 131I is effective in the treatment of RAIR-DTC and is worth using in the clinical practice. In addition, elevated levels of MIP-1α predicted a poor prognosis for patients with RAIR-DTC.

RESUMEN: El objetivo de este estudio fue observar la eficacia clínica de apatinib (AP) combinado con 131I en el tratamiento del cáncer de tiroides diferenciado refractario al yodo radiactivo (RAIR-DTC) y la importancia pronóstica de MIP-1α después del tratamiento, y proporcionar referencia y orientación para futuros tratamientos y enfermedades. Evaluación de RAIR- DTC. Se seleccionaron para el estudio 106 pacientes con RAIR- DTC ingresados en nuestro hospital desde enero de 2019 hasta octubre de 2020. Todos los pacientes fueron tratados con cirugía CT con 131I, y 58 de ellos fueron trasladados posteriormente a tratamiento AP, los que fueron considerados como grupo de investigación; los otros 48 pacientes fueron transferidos a tratamiento de supresión de la hormona estimulante de la tiroides (TSH), que se consideró como grupo de control. La eficacia clínica del grupo de investigación fue mejor que la del grupo de control (P < 0,05), mientras que no se observaron diferencias en la comparación de la incidencia de efectos adversos y la función tiroidea (P > 0,05). Después del tratamiento, Tg, TL, diámetro máximo de los linfonodos C/B, número linfonodos y número de manchas calcificadas fueron menores en el grupo de investigación que en el grupo de control (P <0,05). El análisis ROC reveló que la sensibilidad predictiva de MIP-1α para el pronóstico de muerte por RAIR-DTC a 3 años en el grupo de pacientes de investigación fue del 84,63 % y la especificidad fue del 72,16 %. AP combinado con 131I es eficaz en el tratamiento del RAIR-DTC y vale la pena utilizarlo en la práctica clínica. Además, los niveles elevados de MIP-1α predijeron un mal pronóstico para los pacientes con RAIR- DTC.

Liver cancer is a malignant tumor found worldwide. mRNA turnover 4 homolog (MRTO4) is highly expressed in hepatocellular carcinoma (HCC) tissues, and we explored its relationship with HCC. All cancer data were downloaded from the Cancer Genome Atlas (TCGA), the Cancer Immune Atlas (TCIA), and the Human Protein Atlas (THPA). Stromal scores, immune scores, and ESTIMATE scores were calculated by “ESTIMATE” R package. Single sample gene set enrichment analysis and CIBERSORT were used to evaluate the immune status and infiltration of cancer tissues. pRRophetic R package was used to predict the half-maximal inhibitory concentration (IC50) of different drugs in each sample. MRTO4 overexpression was associated with poor prognosis in HCC, and positively correlated with the stage and grade of HCC patients. The average immunophenoscore (IPS) of the low MRTO4 group was significantly higher than that of the high MRTO4 group. Tumor microenvironment (TME) scores were significantly higher in the low MRTO4 group than in the high MRTO4 group in HCC. MRTO4 expression was positively correlated with tumor mutation burden (TMB) and was positively correlated with most immune checkpoint gene expressions in HCC. Drug sensitivity analysis showed significantly higher IC50 values for 5-fluorouracil, gemcitabine, and sorafenib in patients with low MRTO4 expression than in those with high MRTO4 expression. MRTO4 acts as an independent prognostic and immunological biomarker and is correlated with clinical stage, tumor grade, and drug sensitivity in HCC. It may serve as a putative therapeutic target and potential biomarker for prognosis of HCC. worldwide MRTO (MRTO4 (HCC tissues TCGA, TCGA , (TCGA) TCIA, TCIA (TCIA) THPA. THPA . (THPA) “ESTIMATE halfmaximal half maximal IC (IC50 IPS (IPS TME (TME TMB (TMB IC5 5fluorouracil, 5fluorouracil fluorouracil 5 fluorouracil, 5-fluorouracil gemcitabine (MRTO (TCGA (TCIA (THPA (IC5 (IC

Abstract Objectives: Allergic diseases and Meniere’s disease found to have a possible link in observational study. However, the potential causal relationship between the two is unclear. Therefore, we aimed to explore the causal relationship between allergic diseases and Meniere’s disease using a new data analysis technique called bidirectional Mendelian randomization study. Method: Summary-level statistics for Meniere’s disease and three allergic diseases (asthma, allergic rhinitis, eczema/dermatitis) were obtained from large-scale genome-wide association studies. The inverse variance weighted method was used as the primary measure, supplemented by MR-Egger regression and the weighted median method. To ensure the reliability of the conclusions, Cochran’s Q, MR-Egger intercept, MR-PRESSO test, leave-one-out test, and MR Steiger test were used. Results: Inverse-variance weighted method showed asthma (p = 0.008, OR = 3.908, 95% CI 1.424 − 10.724, adjust p = 0.024), allergic rhinitis (p = 0.026, OR = 24.714, 95% CI 1.479 − 412.827, adjust_p = 0.026) and eczema/dermatitis (p = 0.019, OR = 3725.954, 95% CI 3.795 to 3,658,399.580, adjust_p = 0.029) all had a significant effect on Meniere’s disease. Reverse Mendelian randomization studies have shown that Meniere’s disease does not increase the risk of three allergic diseases. Sensitivity analysis showed no horizontal pleiotropy and heterogeneity for each trait. Conclusion: Our Mendelian randomization analysis supports a positive causal relationship between three allergic diseases (asthma, allergic rhinitis, eczema/dermatitis) and Meniere’s disease. This suggests that physicians should pay more attention to the Meniere’s patient’s allergy history and consider allergy avoidance as part of their treatment plan. Level of evidence: Mendelian Randomized (MR) studies are second only to randomized controlled trials in terms of the level of evidence. Objectives Menieres Meniere s study However unclear Therefore Method Summarylevel Summary asthma, (asthma eczemadermatitis eczema dermatitis largescale large scale genomewide genome wide measure MREgger Egger conclusions Cochrans Cochran Q intercept MRPRESSO PRESSO leaveoneout leave one out Results Inversevariance Inverse 0008 0 008 0.008 3908 3 908 3.908 95 1424 1 424 1.42 10724 10 724 10.724 0.024, 0024 0.024 , 024 0.024) 0026 026 0.026 24714 24 714 24.714 1479 479 1.47 412827 412 827 412.827 adjustp 0019 019 0.019 3725954 3725 954 3725.954 3795 795 3.79 3658399580 658 399 580 3,658,399.580 0.029 0029 029 trait Conclusion patients patient plan evidence (MR 000 00 0.00 390 90 3.90 9 142 42 1.4 1072 72 10.72 002 0.02 02 2471 2 71 24.71 147 47 41282 41 82 412.82 001 01 0.01 372595 372 3725.95 379 79 3.7 365839958 65 39 58 3,658,399.58 0.0 3.9 14 4 1. 107 7 10.7 247 24.7 4128 8 412.8 37259 37 3725.9 3. 36583995 6 5 3,658,399.5 0. 10. 24. 412. 3725. 3658399 3,658,399. 365839 3,658,399 36583 3,658,39 3658 3,658,3 365 3,658, 36 3,658 3,65 3,6 3,

Abstract Autophagy-related gene (ATG) 5 regulates blood lipids, chronic inflammation, CD4+ T-cell differentiation, and neuronal death and is involved in post-stroke cognitive impairment. This study aimed to explore the correlation of serum ATG5 with CD4+ T cells and cognition impairment in stroke patients. Peripheral blood was collected from 180 stroke patients for serum ATG5 and T helper (Th) 1, Th2, Th17, and regulatory T (Treg) cell detection via enzyme-linked immunosorbent assays and flow cytometry. The Mini-Mental State Examination (MMSE) scale was completed at enrollment, year (Y)1, Y2, and Y3 in stroke patients. Serum ATG5 was also measured in 50 healthy controls (HCs). Serum ATG5 was elevated in stroke patients compared to HCs (P<0.001) and was positively correlated to Th2 cells (P=0.022), Th17 cells (P<0.001), and Th17/Treg ratio (P<0.001) in stroke patients but not correlated with Th1 cells, Th1/Th2 ratio, or Treg cells (all P>0.050). Serum ATG5 (P=0.037), Th1 cells (P=0.022), Th17 cells (P=0.002), and Th17/Treg ratio (P=0.018) were elevated in stroke patients with MMSE score-identified cognition impairment vs those without cognition impairment, whereas Th2 cells, Th1/Th2 ratio, and Treg cells were not different between them (all P>0.050). Importantly, serum ATG5 was negatively linked with MMSE score at enrollment (P=0.004), Y1 (P=0.002), Y2 (P=0.014), and Y3 (P=0.001); moreover, it was positively related to 2-year (P=0.024) and 3-year (P=0.012) MMSE score decline in stroke patients. Serum ATG5 was positively correlated with Th2 and Th17 cells and estimated cognitive function decline in stroke patients. Autophagyrelated Autophagy ATG (ATG lipids inflammation CD4 CD Tcell differentiation poststroke post 18 Th (Th 1 (Treg enzymelinked enzyme cytometry MiniMental Mini Mental (MMSE Y1, Y (Y)1 HCs. . (HCs) P<0.001 P0001 P 0 001 (P<0.001 P=0.022, P0022 P=0.022 , 022 (P=0.022) P<0.001, Th17Treg ThTreg Th1Th2 ThTh Th1/Th all P>0.050. P0050 P>0.050 050 P>0.050) P=0.037, P0037 P=0.037 037 (P=0.037) P=0.002, P0002 P=0.002 002 (P=0.002) P=0.018 P0018 018 (P=0.018 scoreidentified identified Importantly P=0.004, P0004 P=0.004 004 (P=0.004) P=0.014, P0014 P=0.014 014 (P=0.014) P=0.001 (P=0.001) moreover 2year 2 P=0.024 P0024 024 (P=0.024 3year 3 P=0.012 P0012 012 (P=0.012 (Y) (HCs P<0.00 P000 00 (P<0.00 P002 P=0.02 02 (P=0.022 Th1Th P005 P>0.05 05 P003 P=0.03 03 (P=0.037 P=0.00 (P=0.002 P=0.01 P001 01 (P=0.01 (P=0.004 (P=0.014 (P=0.001 (P=0.02 (Y P<0.0 P00 (P<0.0 P=0.0 P>0.0 (P=0.03 (P=0.00 (P=0.0 P<0. P0 (P<0. P=0. P>0. (P=0. P<0 (P<0 P=0 P>0 (P=0 P< (P< P= P> (P= (P

SUMMARY OBJECTIVE: This study aimed to explore and analyze the therapeutic effect of the combination of Bifidobacterium animalis subsp. lactis BB-12® and Lactobacillus rhamnosus GG on underweight and malabsorption in premature infants. METHODS: This is a retrospective study. The clinical data of 68 premature infants admitted to Beijing United Family Hospital (Private Secondary Comprehensive Hospital, Chaoyang District, Beijing, China) from January 2016 to January 2022 were analyzed retrospectively. Preterm infants less than 37 weeks of gestational age admitted to the neonatal intensive care unit were included in the study. Patients with intestinal malformations, necrotizing enterocolitis, etc., who require long-term fasting were excluded. A telephone follow-up was performed 3-6 months after discharge. They were classified as treatment groups A and B according to the treatment plan. The treatment group A included parenteral nutrition, enteral nutrition, etc. In treatment group B, based on treatment group A, the premature infants were treated with Bifidobacterium animalis subsp. lactis BB-12® and Lactobacillus rhamnosus GG. The time to regain birthweight and the weight on day 30 were compared between the two groups, as was the duration of transition from parenteral nutrition to total enteral nutrition. RESULTS: The time of weight regain birthweight in group B was shorter than that in group A (t=-2.560; t=-4.287; p<0.05). The increase of weight on day 30 in group B was significantly higher than that in group A (t=2.591; t=2.651; p<0.05). The time from parenteral nutrition to total enteral nutrition in group B was shorter than that in group A (z=-2.145; z=-2.236; p<0.05). CONCLUSION: In the treatment of premature infants, the combination of Bifidobacterium animalis subsp. lactis BB-12® and Lactobacillus rhamnosus GG can have a better therapeutic effect on the underweight and malabsorption of premature infants, and this treatment method can be popularized in clinics. OBJECTIVE subsp BB12® BB12 BB 12® 12 BB-12 METHODS 6 Private District China 201 202 retrospectively 3 malformations enterocolitis etc longterm long term excluded followup follow up 36 3- discharge plan RESULTS t=2.560 t2560 t t= 2.560 2 560 (t=-2.560 t=4.287 t4287 4.287 4 287 t=-4.287 p<0.05. p005 p p<0.05 . 0 05 p<0.05) t=2.591 t2591 591 (t=2.591 t=2.651 t2651 651 z=2.145 z2145 z z= 2.145 145 (z=-2.145 z=2.236 z2236 2.236 236 z=-2.236 CONCLUSION clinics BB1 1 BB-1 20 t=2.56 t256 2560 2.56 56 (t=-2.56 t=4.28 t428 4287 4.28 28 t=-4.28 p00 p<0.0 t=2.59 t259 59 (t=2.59 t=2.65 t265 65 z=2.14 z214 2145 2.14 14 (z=-2.14 z=2.23 z223 2236 2.23 23 z=-2.23 BB- t=2.5 t25 256 2.5 5 (t=-2.5 t=4.2 t42 428 4.2 t=-4.2 p0 p<0. (t=2.5 t=2.6 t26 z=2.1 z21 214 2.1 (z=-2.1 z=2.2 z22 223 2.2 z=-2.2 t=2. t2 25 2. (t=-2. t=4. t4 42 4. t=-4. p<0 (t=2. z=2. z2 21 (z=-2. 22 z=-2. t=2 (t=-2 t=4 t=-4 p< (t=2 z=2 (z=-2 z=-2 (t=- t=- (t= (z=- z=- (t (z= (z

ABSTRACT Purpose: To investigate the protective effect of breviscapine on myocardial ischemia-reperfusion injury (MIRI) in diabetes rats. Methods: Forty rats were divided into control, diabetes, MIRI of diabetes, and treatment groups. The MIRI of diabetes model was established in the latter two groups. Then, the treatment group was treated with 100 mg/kg breviscapine by intraperitoneal injection for 14 consecutive days. Results: After treatment, compared with MIRI of diabetes group, in treatment group the serum fasting blood glucose, fasting insulin, homeostasis model assessment of insulin resistance, and glycosylated hemoglobin levels decreased, the serum total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol levels decreased, the serum high-density lipoprotein cholesterol level increased, the heart rate decreased, the mean arterial pressure, left ventricular ejection fraction, and fractional shortening increased, the serum cardiac troponin I, and creatine kinase-MB levels decreased, the myocardial tumor necrosis factor α and interleukin-6 levels decreased, the myocardial superoxide dismutase level increased, and the myocardial malondialdehyde level decreased (all P < 0.05). Conclusions: For treating MIRI of diabetes in rats, the breviscapine can reduce the blood glucose and lipid levels, improve the cardiac function, reduce the myocardial injury, and decrease the inflammatory response and oxidative stress, thus exerting the alleviating effect. Purpose ischemiareperfusion ischemia reperfusion (MIRI Methods control groups Then 10 mgkg mg kg 1 days Results resistance triacylglycerol lowdensity low density highdensity high increased pressure fraction I kinaseMB kinase MB interleukin6 interleukin 6 interleukin- all 0.05. 005 0.05 . 0 05 0.05) Conclusions function stress 00 0.0 0.

Abstract Slender reed straws in nature can withstand heavy weight of reed spikes and lateral wind force, and thus show good mechanical properties, especially compression resistance and shear resistance. In the paper, the quasi-static and dynamic mechanical properties of reed straws were studied. Three influencing factors of the straws were set according to the nodes (node-containing, node-free), location section (location 1, 2, 3, 4, 5), and moisture content (low, medium, high). The tensile strength of node-free reed straws with low moisture content ranged from 55.40 to 68.14 MPa at different locations. The axial compressive strength of node-containing straws was higher than that of node-free straws, and the influence of moisture content on the axial compressive strength was more obvious. The radial compressive strength of node-containing straws is 13.00 times higher than that of node-free straws in the corresponding part. The bending strength of straws gradually increases from the top to the root, and it is further enhanced with higher moisture content. The axial impact peak load of node-containing straws is up to 1.27 times that of node-free straws, and the axial impact peak load of straws with high moisture content is 1.40 times higher than that of straws with low moisture content in the corresponding part. The radial impact strength of reed straws gradually increases from the top to the root. In terms of bending impact strength, the impact toughness of reed straws ranges from 0.030 to 0.14 J·cm2, and the specific energy absorption ranges from 1.09 to 4.35 J/kg. Because of the excellent mechanical properties, reed straws provide important material performance data for the engineering field and inspire the design of new engineering materials or structures by improving strength and reducing weight. force paper quasistatic quasi static studied nodecontaining, nodecontaining node containing, containing (node-containing nodefree, nodefree free , node-free) 1 2 3 4 5, 5 5) low, (low medium high. . high) 5540 55 40 55.4 6814 68 14 68.1 locations obvious 1300 13 00 13.0 part root 127 27 1.2 140 1.4 0030 0 030 0.03 014 0.1 Jcm2 Jcm J cm2 cm J·cm2 109 09 1.0 435 35 4.3 Jkg kg J/kg 554 55. 681 6 68. 130 13. 12 1. 003 03 0.0 01 0. J·cm 10 43 4.

ABSTRACT We focus here on the problem of poor real-time performance and low accuracy of seed box allowance detection during wheat sowing operations, and present a real-time weighing system for a seed box allowance. The proposed weighing system obtains the seed box allowance using an ultrasonic sensor and load cell, and displays it in real time via a computer. Based on the proposed weighing system, the weighing structure is also designed for the seed box. At the software level, three filtering methods are proposed to condition the signal, namely mean filtering, median filtering, and the acceleration method, and models of these three filtering algorithms are implemented in MATLAB/Simulink. Experiments to assess the accuracy of the system and filtering algorithm were carried out on the test bench; the results show that the system error is initially in the range −16.3 g to 63.8 g, and is reduced to the range −13.4 g to 15.1 g after correction. No significant difference between the three filtering algorithms is found, but since the acceleration method can improve the response speed of the system, this is selected as the final filtering method. realtime operations cell computer level signal MATLABSimulink MATLAB Simulink MATLAB/Simulink bench 163 16 3 −16. 638 63 8 63. 134 13 4 −13. 151 15 1 15. correction found −16 6 −13 −1 −

Abstract Syringa oblata is an important garden plant whose leaf colour turns from green to red in autumn when air temperature and daylength decrease. This study explored the reasons for leaf reddening by detecting phenotypic characteristics and pigment types and contents. With leaf reddening, luminance L* increased and chrominance a* decreased significantly. Chlorophyll and carotenoid contents significantly decreased in accordance with the distribution change of green pigment in leaf cells. Conversely, the red pigment distribution increased and the total polyphenol, total flavonoid and total anthocyanin contents evidently increased. Anthocyanin accumulation was the important reason for leaf reddening. Of the anthocyanins detected in leaves, cyanidin and delphinidin-3-O-rutinoside contents gradually increased with leaf reddening and were negatively correlated with L*. They were considered key anthocyanins influencing leaf colour. Apigenin and syringic acid were correlated with delphinidin-3-O-rutinoside and cyanidin, and they could be the anthocyanin co-pigments. Cyanidin-3-O-arabinoside and taxifolin were more abundant polyphenols in leaves. In summary, anthocyanin accumulation and chlorophyll degradation occurred along with leaf reddening. Temperature, light, and other co-pigments influenced the anthocyanin and chlorophyll contents. This study provides evidence for applications of S. oblata as a coloured-leaf plant in gardens and as a source of active ingredients in the commercial market. decrease L cells Conversely polyphenol leaves delphinidin3Orutinoside delphinidinOrutinoside delphinidin 3 O rutinoside copigments. copigments co pigments. pigments Cyanidin3Oarabinoside CyanidinOarabinoside Cyanidin arabinoside summary Temperature light S colouredleaf coloured market Orutinoside Oarabinoside

The overexpression of P-glycoprotein (P-gp/ABCB1) is a leading cause of multidrug resistance (MDR). Hence, it is crucial to discover effective pharmaceuticals that counteract ABCB1-mediated multidrug resistance. FRAX486 is a p21-activated kinase (PAK) inhibitor. The objective of this study was to investigate whether FRAX486 can reverse ABCB1-mediated multidrug resistance, while also exploring its mechanism of action. The CCK8 assay demonstrated that FRAX486 significantly reversed ABCB1-mediated multidrug resistance. Furthermore, western blotting and immunofluorescence experiments revealed that FRAX486 had no impact on expression level and intracellular localization of ABCB1. Notably, FRAX486 was found to enhance intracellular drug accumulation and reduce efflux, resulting in the reversal of multidrug resistance. Docking analysis also indicated a strong affinity between FRAX486 and ABCB1. This study highlights the ability of FRAX486 to reverse ABCB1-mediated multidrug resistance and provides valuable insights for its clinical application. Pglycoprotein P glycoprotein Pgp/ABCB1 PgpABCB1 PgpABCB gp/ABCB1 gp ABCB1 ABCB (P-gp/ABCB1 MDR. MDR . (MDR) Hence ABCB1mediated ABCBmediated mediated FRAX FRAX48 p21activated pactivated p21 activated p PAK (PAK inhibitor action CCK Furthermore Notably efflux application Pgp Pgp/ABCB gpABCB1 gpABCB gp/ABCB (P-gp/ABCB (MDR FRAX4 p2

Abstract: Confucian thought provides guidance on social structure and political governance. It was introduced to Japan as early as the fourth century AD (Anno Domini) and has been deeply imprinted in Japan’s history and culture for thousands of years. This article takes I am a cat as a specific example to analyze the specific Confucian ideas reflected in Japanese relational language. I am a cat is a classic work by the famous modern Japanese satirist Soseki Natsume. From the specific reflection of I am a cat, Japanese relational language includes Confucian concepts of superiority and inferiority, loyalty and filial piety, harmony and gratitude, as well as the concepts of propriety, benevolence and righteousness. It also reflects that Confucian philosophy has penetrated into Japanese related language. In such research, Japanese learners can gain a more intuitive understanding of the impact of Confucianism on modern Japanese relational language to improve the learning effectiveness. It also helps to develop the understanding of the influence of Confucian culture on Japan. Abstract governance Anno Domini Japans s years Natsume inferiority piety gratitude propriety righteousness research effectiveness

Resumo: O pensamento confucionista fornece orientação sobre a estrutura social e a governança política. Ele foi introduzido no Japão já no século IV d.C. (Anno Domini) e está profundamente marcado na história e na cultura do país, há milhares de anos. Este artigo toma I am a cat como um exemplo específico para analisar as ideias confucionistas específicas refletidas na linguagem relacional japonesa. I am a cat é uma obra clássica do famoso satirista japonês moderno Soseki Natsume. A partir da reflexão específica de I am a cat, a linguagem relacional japonesa inclui conceitos confucionistas de superioridade e inferioridade, lealdade e piedade filial, harmonia e gratidão, bem como os conceitos de propriedade, benevolência e retidão. Isso também reflete que a filosofia confucionista penetrou na linguagem japonesa relacionada. Nesta pesquisa, os alunos de japonês podem obter uma compreensão mais intuitiva do impacto do confucionismo na linguagem relacional japonesa moderna, para melhorar a eficácia do aprendizado. Isso também ajuda a desenvolver a compreensão da influência da cultura confucionista no Japão. Resumo política dC d C d.C Anno Domini país anos Natsume inferioridade filial gratidão propriedade retidão relacionada pesquisa moderna aprendizado

Silicosis is a systemic disease caused by long-term exposure to high concentrations of free silica dust particles in the workplace. It is characterized by a persistent inflammatory response, fibroblast proliferation, and excessive collagen deposition, leading to pulmonary interstitial fibrosis. Epithelial interstitial transformation (EMT) can cause epithelial cells to lose their tight junctions, cell polarity, and epithelial properties, thereby enhancing the properties of interstitial cells, which can lead to the progression of fibrosis and the formation of scar tissue. Integrin 1 (ITGB1) is considered an important factor for promoting EMT and tumor invasion in a variety of tumors and also plays an important role in the progression of fibrotic diseases. Therefore, ITGB1 can be used as a potential target for the treatment of silicosis. In this study, we found that silica exposure induced epithelial-mesenchymal transformation in rats and that the expression of integrin ITGB1 was elevated along with the EMT. We used CRISPR/Cas9 technology to construct integrin ITGB1 knockdown cell lines for in vitro experiments. We compared the expression of the EMT key proteins E-cadherin and vimentin in the ITGB1 knockdown cells and wild-type cells simultaneously stimulated by silica and detected the aggregation point distribution of E-cadherin and vimentin in the cells using laser confocal microscopy. Our results showed that ITGB1 knockout inhibited the ITGB1/ILK/Snail signaling pathway and attenuated the EMT occurrence compared to control cells. These results suggested that ITGB1 is associated with silica-induced EMT and may be a potential target for the treatment of silicosis. longterm long term workplace response proliferation deposition (EMT junctions polarity tissue ITGB (ITGB1 diseases Therefore silicosis study epithelialmesenchymal mesenchymal CRISPRCas9 CRISPRCas CRISPR Cas9 Cas CRISPR/Cas experiments Ecadherin E cadherin wildtype wild type microscopy ITGB1ILKSnail ITGBILKSnail ILK Snail silicainduced (ITGB ILKSnail