ABSTRACT: This study detected Anaplasma marginale in calvesusing blood smears and nested PCR (nPCR) and to compare the results with the clinical signs presented by calves on a dairy farm in the municipality of Castanhal, located northeast of the state Pará (1°07’19.1”S and 47°53’53.0”W), eastern Amazon. To this end, 192 blood samples were collected from 24 animals at 1-20, 21-41 and 42-60 days of age. Blood smears and nPCR with primers for the msp5 gene were performed. The prevalence of A. marginale was 61.5% (118/192) for the blood smear technique and nPCR (msp5). The manifestation of clinical signs of anaplasmosis also increased significantly over the course of the study (P < 0.0001), being lower in animals aged 1-20 days, but increasing among those aged 21-41 and 42-60 days. These signs were characterized by apathy, fever, weight loss, diarrhea, dehydration, and hypochromic mucous membranes. Regarding the evaluation of the diagnostic techniques, no significant difference was observed in the detection of A. marginale between the blood smear and nPCR (P = 0.995), but the agent’s rickets increased on Day 47 (P < 0.01) in both tests, thereby demonstrating a near-linear pattern of increase in rickets over the 60 days, with a consequent decrease in globular volume. This shows that of the 24 animals studied, 21 were infected at some point during the study period. Additionally, there was no significant difference between blood smears and nPCR, probably due to medium and high parasitemia, which were directly related to the clinical signs and decrease in globular volume. ABSTRACT (nPCR Castanhal 1°0719.1S 107191S S 1°07 19.1 1 07 19 47°5353.0W, 4753530W W 47°53 53.0 , 53 0 47°53’53.0”W) Amazon end 2 120, 120 20, 20 2141 41 21-4 4260 42 42-6 age msp performed A 615 61 5 61.5 118/192 118192 118 (118/192 msp5. . (msp5) P 0.0001, 00001 0.0001 0001 0.0001) 1-2 apathy fever loss diarrhea dehydration membranes techniques 0.995, 0995 0.995 995 0.995) agents agent s 4 0.01 001 01 tests nearlinear near linear 6 volume studied period Additionally parasitemia 0719 1S 107 1°0 191 19. 5353 0W 47°5353.0W 4753 47°5 530 53. 47°53’53.0”W 12 214 21- 426 42- 61. 118/19 11819 11 (118/19 (msp5 0000 0.000 000 1- 099 0.99 99 0.0 00 071 10 1° 535 475 47° 118/1 1181 (118/1 (msp 0.00 09 0.9 9 0. 118/ (118/ (118 (11 (1 (

RESUMO: Objetivou-se, na construção deste trabalho, detectar Anaplasma marginale por meio do esfregaço sanguíneo e Nested PCR (nPCR) e comparar os resultados com os sinais clínicos apresentados pelos bezerros em uma propriedade leiteira localizada no município de Castanhal, região nordeste do estado do Pará (1°07’19,1”S e 47°53’53,0”W), Amazônia Oriental. Para isso, foram coletadas 192 amostras sanguíneas de 24 animais, divididos em três períodos: 1-20, 21-41 e 42-60 dias de idade. Foram realizados esfregaços sanguíneos e nPCR com iniciadores para o gene msp5. A prevalência de A. marginale foi de 61,46% (118/192) tanto para a técnica de esfregaço sanguíneo quanto para nPCR (msp5). A manifestação de sinais clínicos da anaplasmose também foi significativamente crescente ao longo do estudo (P < 0,0001), sendo menor em animais de 1 a 20 dias, mas expandindo-se entre os de 21 a 41 dias e 42 a 60 dias, esses sinais foram caracterizados por apatia, febre, perda de peso, diarreia, desidratação e mucosas hipocoradas. Quanto a avaliação das técnicas diagnósticas, não houve diferença significativa entre a detecção de A. marginale no esfregaço sanguíneo e na nPCR (P = 0,995), porém se observou aumento riquetsêmico do agente no 47º dia (P < 0,01) em ambos os testes, demonstrando, assim, um padrão de aumento da riquetsemia próximo ao linear ao longo dos 60 dias, com consequente diminuição do volume globular. Assim, demonstra-se que dos 24 animais estudados, 21 se infectaram em algum momento do período estudado, e não houve diferença significativa entre esfregaço sanguíneo e nPCR, em virtude, provavelmente, das parasitemias médias e altas, as quais estiveram diretamente relacionadas com os sinais clínicos e a diminuição do volume globular. RESUMO Objetivouse, Objetivouse Objetivou se, Objetivou-se trabalho (nPCR Castanhal 1°0719,1S 107191S S 1°07 19,1 07 19 47°5353,0W, 4753530W W 47°53 53,0 , 47 53 0 47°53’53,0”W) Oriental isso 2 períodos 120, 120 20, 1-20 2141 21-4 4260 42-6 idade msp5 msp 6146 61 46 61,46 118/192 118192 118 (118/192 . (msp5) P 0,0001, 00001 0,0001 0001 0,0001) expandindose expandindo 4 6 apatia febre peso diarreia hipocoradas diagnósticas 0,995, 0995 0,995 995 0,995) º 0,01 001 01 testes demonstrando assim globular Assim demonstrase demonstra estudados estudado virtude provavelmente altas 0719 1S 107 1°0 191 19, 5353 0W 47°5353,0W 4753 47°5 530 53, 5 47°53’53,0”W 12 1-2 214 21- 426 42- 614 61,4 118/19 11819 11 (118/19 (msp5 0000 0,000 000 099 0,99 99 0,0 00 071 10 1° 535 475 47° 1- 61, 118/1 1181 (118/1 (msp 0,00 09 0,9 9 0, 118/ (118/ (118 (11 (1 (

Abstract Objective This study evaluated the color stability of enamel submitted to 10% hydrogen peroxide (HP) followed by antioxidants agents, and the pH and antioxidant activity (AA%) of these agents. Methodology Bovine enamel-dentin blocks were randomly distributed into groups (n=10/group): GNC (negative control: no treatment); GPC (positive control: bleaching only); TOC_10% (HP+10% α-tocopherol); GT_10% (HP+10% green tea extract); GS_5% (HP+5% grape seed extract); SA_10% (HP+10% sodium ascorbate); QUI_10% (HP+10% quinoa extract); and QC_1% (HP+1% quercetin). Color (ΔE00) and whiteness index (ΔWID) changes were analyzed using a digital spectrophotometer. The pH and AA% were determined using a pH meter and the DPPH method, respectively. Data were analyzed by ANOVA/Tukey’s and Dunnett’s tests (α=0.05). Results At 14 days post-bleaching, GNC promoted the lowest ΔWID and ΔE00 (p<0.05), and no differences were found between GPC and the remaining groups submitted to the antioxidant agents (p>0.05). QC_1% and QUI_10% exhibited acidic pH levels (3.64 and 4.75, respectively), whereas TOC_10% and GS_5% exhibited alkaline pH (7.07 and 7.64, respectively). No differences in AA% were found between the agents (p>0.05), ranging from 92.6 to 97.6%. Conclusion The antioxidant agents did not interfere in bleached enamel color stability, showing satisfactory antioxidant activity. However, QUI and QC gels displayed acidic pH. Clinical significance: The antioxidants evaluated showed high AA% and no impact on post-bleaching color stability, suggesting that their capacity to recover bond strength demonstrated elsewhere would not compromise the esthetic efficacy of tooth bleaching. However, those with acidic pH should be used with caution due to potential enamel damage. 10 HP (HP AA (AA% enameldentin dentin n=10/group n10group ngroup n group (n=10/group) negative control treatment treatment) positive only only) TOC10 TOC TOC_10 HP+10% HP10 (HP+10 αtocopherol α tocopherol α-tocopherol) GT10 GT GT_10 extract extract) GS5 GS 5 GS_5 HP+5% HP5 (HP+5 SA10 SA SA_10 ascorbate ascorbate) QUI10 QUI_10 QC1 1 QC_1 HP+1% HP1 (HP+1 quercetin. quercetin . quercetin) ΔE (ΔE00 (ΔWID spectrophotometer method respectively ANOVA/Tukeys ANOVATukeys ANOVA/Tukey s ANOVA Tukey Dunnetts Dunnett α=0.05. α005 α=0.05 0 05 (α=0.05) postbleaching, postbleaching post bleaching, ΔE0 p<0.05, p005 p p<0.05 , (p<0.05) p>0.05. p>0.05 (p>0.05) 3.64 364 3 64 (3.6 475 4 75 4.75 respectively, respectively) 7.07 707 7 07 (7.0 764 7.64 p>0.05, 926 92 6 92. 976 97 97.6% However significance damage (AA (n=10/group TOC1 TOC_1 HP+10 α-tocopherol GT1 GT_1 GS_ HP+5 (HP+ SA1 SA_1 QUI1 QUI_1 QC_ HP+1 (ΔE0 Tukeys ANOVATukey α00 α=0.0 (α=0.05 p00 p<0.0 (p<0.05 p>0.0 (p>0.05 3.6 36 (3. 47 4.7 7.0 70 (7. 76 7.6 9 97.6 TOC_ GT_ HP+ SA_ QUI_ (ΔE α0 α=0. (α=0.0 p0 p<0. (p<0.0 p>0. (p>0.0 3. (3 4. 7. (7 97. α=0 (α=0. p<0 (p<0. p>0 (p>0. ( α= (α=0 p< (p<0 p> (p>0 (α= (p< (p> (α (p

ABSTRACT: The objective of the present study was to detect the genetic diversity of Anaplasma marginale strains in naturally infected calves from a rural property located in the northeastern region of the state of Pará, Eastern Amazon, which has a history of mortality due to anaplasmosis. Fourteen calves positive for A. marginale were selected using a semi-nested polymerase chain reaction for the target msp1α gene, with asymptomatic (n=3) and symptomatic (n=11) infections. After sequencing the samples, two genotypes were verified in the E and C regions and the structures in tandem repeats were determined. Nine different strains were found: eight related to the E genotype (α-β-β-Γ = one animal, asymptomatic; 16-F-17-F-F = two animals, symptomatic; α-β-F-F-F-F = one animal, asymptomatic; 31-62-62-61 = one animal, symptomatic; τ-10-3 = three animals, two symptomatic and one asymptomatic; α-β-β-β = one animal, symptomatic; τ-22 -13-18 = two animals, both symptomatic; β-β-β-BRA1-31 = two animals, both symptomatic), and one related to genotype C (23-24-25-31-27-27 = one animal, asymptomatic). Genotype E was predominant in 92.86% of the samples (13/14), followed by genotype C (7.14%). This study made it possible to detect the genetic diversity of A. marginale in calves from the selected dairy farm, in addition to identifying the BRA1 sequence in the animals of the present study, which was recently diagnosed in Minas Gerais, demonstrating the dispersion of A. marginale strains in herds from different Brazilian states. Genetic diversity of A. marginale was observed in both symptomatic and asymptomatic calves. There were no significant differences when clinical signs were compared to the genotype verified in the infected animals. The prevalence of pathogenicity was not observed. ABSTRACT Pará Amazon anaplasmosis A seminested semi nested mspα msp α gene n=3 n3 n 3 (n=3 n=11 n11 11 (n=11 infections determined found αββΓ β Γ animal 16F17FF FFF 16 F 17 αβFFFF 31626261 31 62 61 31-62-62-6 τ103 τ 10 τ-10- αβββ τ22 22 τ-2 1318 13 18 -13-1 βββBRA131 βββBRA BRA β-β-β-BRA1-3 symptomatic, , symptomatic) 232425312727 23 24 25 27 (23-24-25-31-27-2 asymptomatic. . asymptomatic) 9286 92 86 92.86 13/14, 1314 13/14 14 (13/14) 7.14%. 714 7.14% 7 (7.14%) farm Gerais states n= (n= n=1 n1 1 (n=1 FF 3162626 6 31-62-62- τ10 τ-10 τ2 2 τ- 131 -13- βββBRA13 β-β-β-BRA1- 23242531272 (23-24-25-31-27- 928 9 8 92.8 13/1 (13/14 71 7.14 (7.14% (n 316262 31-62-62 τ1 τ-1 -13 βββBRA1 β-β-β-BRA1 2324253127 (23-24-25-31-27 92. 13/ (13/1 7.1 (7.14 31626 31-62-6 -1 β-β-β-BRA 232425312 (23-24-25-31-2 (13/ 7. (7.1 3162 31-62- - 23242531 (23-24-25-31- (13 (7. 316 31-62 2324253 (23-24-25-31 (1 (7 31-6 232425 (23-24-25-3 ( 31- 23242 (23-24-25- 2324 (23-24-25 232 (23-24-2 (23-24- (23-24 (23-2 (23- (23 (2

RESUMO: O objetivo do presente trabalho foi detectar a diversidade genética de cepas de Anaplasma marginale em bezerros naturalmente infectados oriundos de uma propriedade rural localizada na região nordeste do estado do Pará, Amazônia Oriental, a qual apresentava histórico de mortalidade devido à anaplasmose. Foram selecionados 14 bezerros positivos para A. marginale pela técnica de semi-nested PCR (nPCR) para o alvo no gene msp1α, com infecção assintomática (n=3) e sintomáticos (n=11). Após o sequenciamento das amostras foram verificados dois genótipos nas regiões E e C, e determinadas as estruturas em tandem repeats. Nove diferentes estirpes foram encontradas, sendo oito relacionadas ao genótipo E (α-β-β-Γ = um animal, assintomático; 16-F-17-F-F = dois animais, sintomáticos; α-β-F-F-F-F = um animal, assintomático; 31-62-62-61 = um animal, sintomático; τ-10-3 = três animais, dois sintomáticos e um assintomático; α-β-β-β = um animal, sintomático; τ-22-13-18 = dois animais, sintomáticos; β-β-β-BRA1-31 = dois animais, sintomáticos) e uma relacionada ao genótipo C (23-24-25-31-27-27 = um animal, assintomático). O genótipo E foi predominante em 92,86% das amostras (13/14), seguido pelo genótipo C (7,14%). O estudo possibilitou a detecção da diversidade genética de A. marginale em bezerros dessa propriedade leiteira, além de identificar a sequência BRA1 nos animais do presente estudo, a qual foi diagnosticada recentemente em Minas Gerais, o que demonstra a dispersão das estirpes de A. marginale nos rebanhos de diferentes estados brasileiros. A diversidade genética de A. marginale foi observada tanto em bezerros sintomáticos quanto em assintomáticos e não houve diferença significativa quando se comparou os sinais clínicos ao genótipo verificado no animal infectado, não observando a prevalência de patogenicidade de estirpes. RESUMO Pará Oriental anaplasmose 1 seminested semi nested nPCR (nPCR msp1α mspα msp α n=3 n3 n 3 (n=3 n=11. n11 n=11 . 11 (n=11) repeats encontradas αββΓ β Γ assintomático 16F17FF FFF 16 F 17 αβFFFF 31626261 31 62 61 31-62-62-6 sintomático τ103 τ 10 τ-10- αβββ τ221318 22 13 18 τ-22-13-1 βββBRA131 βββBRA BRA β-β-β-BRA1-3 232425312727 23 24 25 27 (23-24-25-31-27-2 assintomático. assintomático) 9286 92 86 92,86 13/14, 1314 13/14 , (13/14) 7,14%. 714 7,14% 7 (7,14%) leiteira Gerais brasileiros infectado n= (n= n1 n=1 (n=11 FF 3162626 6 31-62-62- τ10 τ-10 τ22131 2 τ-22-13- βββBRA13 β-β-β-BRA1- 23242531272 (23-24-25-31-27- 928 9 8 92,8 131 13/1 (13/14 71 7,14 (7,14% (n (n=1 316262 31-62-62 τ1 τ-1 τ2213 τ-22-13 βββBRA1 β-β-β-BRA1 2324253127 (23-24-25-31-27 92, 13/ (13/1 7,1 (7,14 31626 31-62-6 τ- τ221 τ-22-1 β-β-β-BRA 232425312 (23-24-25-31-2 (13/ 7, (7,1 3162 31-62- τ22 τ-22- 23242531 (23-24-25-31- (13 (7, 316 31-62 τ2 τ-22 2324253 (23-24-25-31 (1 (7 31-6 τ-2 232425 (23-24-25-3 ( 31- 23242 (23-24-25- 2324 (23-24-25 232 (23-24-2 (23-24- (23-24 (23-2 (23- (23 (2

ABSTRACT Background: Patauá (Oenocarpus bataua Mart.) is a palm tree belonging to the botanical family Arecaceae that occurs throughout the Amazon. Like açaí, an edible pulp is extracted from its fruits, remaining a fiber-rich waste. Revealing the potential of such raw or thermochemically modified fibers for producing bioproducts is a novelty in the literature. Therefore, this work aimed to characterize patauá fibers in natural and alkali-treated conditions to support future bioproduct applications. Alkaline treatments were performed under mechanical stirring combining two NaOH levels (5 and 10%) and two temperatures (80 and 100°C). Morphological characterization was performed by light microscopy and scanning electron microscopy (SEM). The contents of the structural and non-structural chemical components were determined, and chemical groups were evaluated by Fourier-transform infrared spectroscopy (FTIR). The physical characterization included moisture content, apparent density, and water absorption tests. Results: The macerate revealed short fibers with typical elongated morphology, mean cell wall thickness (4.10 µm) greater than the mean lumen width (3.01 µm), and mean length of 445 µm. The alkaline treatments partially individualized the fibers from bundles, cleaned extractives from the surface, and unblocked superficial pits by removing silica-rich structures. They substantially removed non-cellulosic components, but FTIR showed condensed lignin exposed on the fiber surface. Density and water uptake increased concerning natural fibers. Patauá’s short length and susceptibility to modification through thermochemical treatments that concentrated cellulose up to 50% indicated great potential for developing composites and nanofibers. Regardless of the NaOH content, 100°C was more efficient in concentrating cellulose. Conclusion: Patauá fibers have unique natural characteristics. They occur as flatted fiber bundles, have silicon-rich structures obstructing their superficial pits, and comprise more lignin than cellulose. Background Oenocarpus Mart. Mart Amazon açaí fruits fiberrich rich waste literature Therefore alkalitreated alkali treated applications 5 ( 10% 10 80 (8 100°C. 100C C . 100 100°C) SEM. SEM (SEM) nonstructural non determined Fouriertransform Fourier transform FTIR. (FTIR) content density tests Results morphology 4.10 410 4 (4.1 µm 3.01 301 3 01 (3.0 µm, , 44 bundles surface silicarich silica noncellulosic cellulosic Patauás s 50 nanofibers Conclusion characteristics siliconrich silicon 1 8 (SEM (FTIR 4.1 41 (4. 3.0 30 0 (3. 4. (4 3. (3

RESUMEN Objetivo: Estudiar el impacto de la pandemia de COVID-19 en las cirugías de columna en un Centro de Referencia para Urgencias de Alta Complejidad en Goiás. Métodos: Se realizó un estudio observacional retrospectivo a partir de datos de historias clínicas de pacientes operados de la columna entre septiembre de 2017 y septiembre de 2021. Los voluntarios se dividieron en dos grupos: antes de la pandemia y durante la pandemia, considerando abril de 2020 como punto de partida. De las historias clínicas se extrajo: edad, sexo, escolaridad, etiología, déficit neurológico, región de la columna abordada, mecanismo del trauma, vértebras fracturadas, tipo de fractura, tiempo de estancia en la UCI, test COVID (PCR) y fallecidos. Se utilizaron las pruebas estadísticas t de Student, chi-cuadrado y exacta de Fisher para comparar el perfil pre y postpandemia. Además, se aplicó la prueba de correlación de Spearman para verificar la correlación entre las variables, considerando p<0,05. Resultados: se analizaron 388 historias clínicas, mostrando un aumento del 15% en las cirugías de columna durante la pandemia. Hubo una diferencia significativa en la etiología de las cirugías (p=0,05), siendo más frecuente el traumatismo lumbar en los hombres y también más casos de déficit neurológico (p=0,001). También hubo una reducción en la estancia en la UTI (p=0,0001), que fue menor durante la pandemia. Conclusión: La pandemia de COVID-19 no impactó directamente en el número de cirugías realizadas en un Hospital de Referencia en Urgencias de Alta Complejidad en Goiás, pero hubo una priorización de las cirugías de emergencia. Nivel de Evidencia II; Estudio Retrospectivo. Objetivo COVID19 19 COVID-1 Goiás Métodos 201 2021 grupos 202 partida extrajo edad sexo escolaridad abordada trauma fracturadas fractura UCI PCR (PCR fallecidos Student chicuadrado chi cuadrado postpandemia Además variables p005 p 0 05 p<0,05 Resultados 38 15 p=0,05, p=0,05 , (p=0,05) p=0,001. p0001 p=0,001 . 001 (p=0,001) p=0,0001, p00001 p=0,0001 0001 (p=0,0001) Conclusión emergencia II Retrospectivo COVID1 1 COVID- 20 p00 p<0,0 3 p=0,0 (p=0,05 p000 p=0,00 00 (p=0,001 p0000 p=0,000 000 (p=0,0001 2 p0 p<0, p=0, (p=0,0 (p=0,00 (p=0,000 p<0 p=0 (p=0, p< p= (p=0 (p= (p

ABSTRACT Objective: To study the impact of the COVID-19 pandemic on spine surgeries at a Reference Center for High Complexity Urgencies in Goiás. Methods: A retrospective, observational study was carried out based on data from medical records of patients undergoing spine surgery between September 2017 and September 2021. Volunteers were divided into two groups: before and during the pandemic, considering April 2020 as a starting point. The following was extracted from the medical records: age, gender, education, etiology, neurological deficit, region of the spine addressed, mechanism of trauma, fractured vertebrae, type of fracture, length of stay in the ICU, COVID test (PCR), and deaths. Student t, chi-square, and Fisher’s exact statistical tests were used to compare the preand post-pandemic profiles. In addition, Spearman’s correlation test was applied to verify the correlation between variables, considering p<0.05. Results: 388 medical records were analyzed, showing a 15% increase in spine surgeries during the pandemic. There was a significant difference in the etiology of surgeries (p=0.05), with lumbar trauma being more prevalent in men and also more cases of neurological deficits (p=0.001). There was also a reduction in the length of stay in the ICU (p=0.0001), which was lower during the pandemic. Conclusion: The COVID-19 pandemic did not directly impact the number of surgeries performed at a Reference Hospital in High Complexity Urgencies in Goiás, but there was a prioritization of emergency surgeries. Level of Evidence II; Retrospective Study. Objective COVID19 19 COVID-1 Goiás Methods retrospective 201 2021 groups 202 point age gender education deficit addressed vertebrae fracture PCR, PCR , (PCR) deaths t chisquare, chisquare chi square, square chi-square Fishers Fisher s postpandemic post profiles addition Spearmans Spearman variables p005 p 0 05 p<0.05 Results 38 analyzed 15 p=0.05, p=0.05 (p=0.05) p=0.001. p0001 p=0.001 . 001 (p=0.001) p=0.0001, p00001 p=0.0001 0001 (p=0.0001) Conclusion II Study COVID1 1 COVID- 20 (PCR p00 p<0.0 3 p=0.0 (p=0.05 p000 p=0.00 00 (p=0.001 p0000 p=0.000 000 (p=0.0001 2 p0 p<0. p=0. (p=0.0 (p=0.00 (p=0.000 p<0 p=0 (p=0. p< p= (p=0 (p= (p

RESUMO Objetivo: Estudar o impacto da pandemia de COVID-19 nas cirurgias de coluna em um Centro de Referência em Urgências de Alta Complexidade em Goiás. Métodos: Foi realizado um estudo retrospectivo, observacional a partir de dados de prontuários médicos de pacientes submetidos à cirurgia de coluna entre setembro de 2017 a setembro de 2021. Os voluntários foram divididos em dois grupos: antes da pandemia e durante a pandemia, considerando abril de 2020 como marco de início. Foram extraídos dos prontuários: idade, gênero, escolaridade, etiologia, déficit neurológico, região da coluna abordada, mecanismo de trauma, vértebras fraturadas, tipo de fratura, tempo de internação em UTI, teste de COVID (PCR) e óbitos. Utilizou-se os testes estatísticos T de Student, Qui quadrado e exato de Fisher para realizar a comparação entre o perfil pré e pós-pandemia. Além disso, foi aplicado o teste de correlação de Spearman para verificar a correlação entre as variáveis, considerando p<0,05. Resultados: Foram analisados 388 prontuários, constatando um aumento de 15% nas cirurgias de coluna durante a pandemia. Houve diferença significativa na etiologia das cirurgias (p=0,05), sendo mais prevalentes traumas lombares em homens e também mais casos de déficits neurológicos (p=0,001). Notou-se também uma redução no tempo de internação na UTI (p=0,0001), que foi menor durante a pandemia. Conclusão: A pandemia de COVID-19 não impactou diretamente na quantidade de cirurgias realizadas em um Hospital de Referência em Urgências de Alta Complexidade em Goiás, mas verificou-se uma priorização das cirurgias emergenciais. Nível de Evidência II; Estudo Retrospectivo. Objetivo COVID19 19 COVID-1 Goiás Métodos retrospectivo 201 2021 grupos 202 início idade gênero escolaridade neurológico abordada trauma fraturadas fratura PCR (PCR óbitos Utilizouse Utilizou se Student póspandemia. póspandemia pós pós-pandemia disso variáveis p005 p 0 05 p<0,05 Resultados 38 15 p=0,05, p=0,05 , (p=0,05) p=0,001. p0001 p=0,001 . 001 (p=0,001) Notouse Notou p=0,0001, p00001 p=0,0001 0001 (p=0,0001) Conclusão verificouse verificou emergenciais II Retrospectivo COVID1 1 COVID- 20 p00 p<0,0 3 p=0,0 (p=0,05 p000 p=0,00 00 (p=0,001 p0000 p=0,000 000 (p=0,0001 2 p0 p<0, p=0, (p=0,0 (p=0,00 (p=0,000 p<0 p=0 (p=0, p< p= (p=0 (p= (p

ABSTRACT Resistance training (RT) is a widely practised type of training, and the number of adherents of this type of physical exercise is increasing each year. Among the most sought objectives of those who initiate RT is muscle hypertrophy, although success in this process depends on a well-designed protocol and good manipulation of training variables. The present study aims to analyse, through a systematic review, the impact of the RT variables (such as intensity, volume, recovery interval, execution speed, and concentric muscle failure) relevant to muscle hypertrophy and if there is an ideal range for each training variable. The research was carried out in the PubMed, Web of Science, Scopus and Scielo databases from 2000 to 2020, using the terms “resistance training” and “hypertrophy” and “intensity” or “volume” or “recovery interval” or “execution speed” or “muscular failure”. Twenty-three articles were included in the review. The PEDro scale was used to analyse the quality of the selected articles. It was concluded that the variables intensity and volume must be carefully analysed in a training program. Despite not having a direct impact on hypertrophy, the other variables affect the intensity and volume and must be manipulated according to what is intended with the others.

Abstract Background Aphasia, the most common language disorder secondary to stroke, has been associated with increased mortality, longer hospitalization and rehabilitation times, worse performance in daily activities, increased financial burden, and short- and long-term complications. Aphasia can negatively impact functional communication skills, including social networks, social activities, relationships with other people and social support. Objective To evaluate patients with poststroke aphasia in their respective residences to investigate potential predictors of functional communication. Methods The prospective cohort included patients with poststroke aphasia aged 18 years or older who resided in the city of Salvador, Northeastern Brazil. Following discharge from the Stroke Unit (SU), the individuals themselves, or their guardians, were contacted by telephone to schedule a home visit no less than three months after discharge. At baseline, sociodemographic and clinical data were collected, in addition to the scores on the National Institutes of Health Stroke Scale (NIHSS) and modified Barthel Index (mBI). The American Speech-Language-Hearing Association Functional Assessment of Communication Skills for Adults (ASHA FACS) was applied at the patients’ homes. Multivariate linear regression was employed using the total score on the ASHA FACS as the outcome of interest. Results A multivariate analysis of the associated factors identified using the linear regression revealed that only functional capacity (as assessed by the mBI) upon discharge from the SU remained as an independent predictor of functional communication performance (β = 0.042; 95% confidence interval [95%CI] = 0.013–0.071; p = 0.002). Conclusion The functional capacity to perform daily activities, evaluated upon discharge from a stroke unit, was identified as a potential predictor of functional communication performance, regardless of the time elapsed after the stroke. mortality times activities burden short longterm long term complications skills networks support 1 Salvador Brazil SU, , (SU) themselves guardians baseline collected NIHSS (NIHSS mBI. mBI . (mBI) SpeechLanguageHearing Speech Language Hearing homes interest β 0.042 0042 0 042 95 95%CI 95CI CI [95%CI 0.013–0.071 00130071 013 071 0.002. 0002 0.002 002 0.002) unit (SU (mBI 0.04 004 04 9 0.013–0.07 0013007 01 07 000 0.00 00 0.0 0.013–0.0 001300 0. 0.013–0. 00130 0.013–0 0013 0.013– 001 0.013 0.01

Resumo Antecedentes A afasia, distúrbio de linguagem mais comum secundário ao acidente vascular cerebral (AVC), está associada ao aumento da mortalidade, a um maior tempo de internação e reabilitação, ao pior desempenho nas atividades diárias, ao aumento da carga financeira, e às complicações de curto e longo prazos. Pode impactar negativamente as habilidades de comunicação funcional, incluindo atividades sociais, relacionamento com outras pessoas, e o apoio social. Objetivo Avaliar pacientes com afasia pós-AVC em suas respectivas residências para investigar potenciais preditores de comunicação funcional. Métodos A coorte prospectiva incluiu pacientes com afasia pós-AVC com 18 anos de idade ou mais, residentes em Salvador, Brasil. Após a alta da Unidade de AVC (UAVC), os próprios indivíduos, ou seus responsáveis, foram contatados por telefone para agendamento de visita domiciliar no mínimo três meses após a alta. Inicialmente, foram coletados dados sociodemográficos e clínicos, além das pontuações na National Institutes of Health Stroke Scale (NIHSS) e no Índice de Barthel modificado (IBM). O American Speech-Language-Hearing Association Functional Assessment of Communication Skills for Adults (ASHA FACS) foi aplicado no domicílio dos pacientes. A regressão linear multivariada foi empregada usando a pontuação total no ASHA FACS como o desfecho de interesse. Resultados A análise multivariada por meio de regressão linear revelou que apenas a capacidade funcional avaliada na alta da UAVC permaneceu como preditor independente do desempenho da comunicação funcional (β = 0,042; intervalo de confiança de 95% [IC95%] = 0,013–0,071; p = 0,002). Conclusão A capacidade funcional para realizar as atividades diárias, avaliada na alta hospitalar, foi identificada como potencial preditor do desempenho da comunicação funcional, independente do tempo desde o AVC. AVC, , (AVC) mortalidade reabilitação diárias financeira prazos sociais pessoas social pósAVC pós 1 Salvador Brasil UAVC, (UAVC) indivíduos responsáveis Inicialmente clínicos NIHSS (NIHSS IBM. IBM . (IBM) SpeechLanguageHearing Speech Language Hearing interesse β 0,042 0042 0 042 95 IC95% IC95 IC [IC95% 0,013–0,071 00130071 013 071 0,002. 0002 0,002 002 0,002) hospitalar (AVC (UAVC (IBM 0,04 004 04 9 IC9 [IC95 0,013–0,07 0013007 01 07 000 0,00 00 0,0 [IC9 0,013–0,0 001300 0, [IC 0,013–0, 00130 0,013–0 0013 0,013– 001 0,013 0,01

Abstract Protease-activated receptors (PARs) are metabotropic G-protein-coupled receptors that are activated via proteolytic cleavage of a specific sequence of amino acids in their N-terminal region. PAR2 has been implicated in mediating allergic airway inflammation. This study aims to study the effect of PAR2 antagonist ENMD1068in lung inflammation and airway remodeling in experimental asthma. Allergic lung inflammation was induced in sensitized BALB/c mice through intranasal instillations of ovalbumin (OVA), and mice were pretreated with ENMD1068 1 hour before each OVA challenge. Bronchoalveolar lavage fluid (BALF) was collected, and the lungs were removed at different time intervals after OVA challenge to analyze inflammation, airway remodeling and airway hyperresponsiveness. Ovalbumin promoted leukocyte infiltration into BALF in a PAR2-dependent manner. ENMD1068 impaired eosinophil peroxidase (EPO) and myeloperoxidase (MPO) activity in the lung parenchyma into BALF and reduced the loss of dynamic pulmonary compliance, lung resistance in response to methacholine, mucus production, collagen deposition and chemokine (C-C motif) ligand 5 expression compared to those in OVA-challenged mice. We propose that proteases released after an allergen challenge may be crucial to the development of allergic asthma in mice, and PAR2 blockade may be useful as a new pharmacological approach for the treatment of airway allergic diseases. Proteaseactivated Protease PARs (PARs Gproteincoupled G protein coupled Nterminal N terminal region PAR ENMDin ENMD BALBc BALB c OVA, , (OVA) ENMD106 (BALF collected hyperresponsiveness PAR2dependent PARdependent dependent manner EPO (EPO MPO (MPO compliance methacholine production CC C motif OVAchallenged challenged diseases (OVA ENMD10 ENMD1

Abstract Zoonotic spillover is a phenomenon characterized by the transfer of pathogens between different animal species. Most human emerging infectious diseases originate from non-human animals, and human-related environmental disturbances are the driving forces of the emergence of new human pathogens. Synthesizing the sequence of basic events involved in the emergence of new human pathogens is important for guiding the understanding, identification, and description of key aspects of human activities that can be changed to prevent new outbreaks, epidemics, and pandemics. This review synthesizes the connections between environmental disturbances and increased risk of spillover events based on the One Health perspective. Anthropogenic disturbances in the environment (e.g., deforestation, habitat fragmentation, biodiversity loss, wildlife exploitation) lead to changes in ecological niches, reduction of the dilution effect, increased contact between humans and other animals, changes in the incidence and load of pathogens in animal populations, and alterations in the abiotic factors of landscapes. These phenomena can increase the risk of spillover events and, potentially, facilitate new infectious disease outbreaks. Using Brazil as a study model, this review brings a discussion concerning anthropogenic activities in the Amazon region and their potential impacts on spillover risk and spread of emerging diseases in this region.

Abstract Curcumin, contained at Turmeric (Curcumalonga), can exert many beneficial pleiotropic activities in the gastrointestinal tract. This study evaluated the antioxidant and anti-inflammatory activity of C. longa on 5-fluorouracil (5-FU)-induced oral mucositis (OM) in hamsters. Phytochemical analysis of crude C. longa extract (CLE) was performed to detect the presence of curcumin by TLC and HPLC. Golden Syrian hamsters were orally pre-treated with CLE (5, 50, or 100mg/kg). Cheek pouch samples were subjected to macroscopic and histopathological evaluation. ELISA was performed to quantify the inflammatory cytokines IL-1β and TNF-α. Superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA) levels were assessed by ultraviolet-visible spectroscopy analysis. Behavior analysis was conducted by the open field test. Curcumin content in the CLE was 0.55%m/m ± 0.0161 (2.84%). The group treated with 5mg/kg CLE showed healing evidence with macroscopic absence of ulceration (p<0.05) and microscopic aspect of re-epithelialization, discrete inflammatory infiltrate and absence of edema. Treatment with 5mg/kg CLE significantly increased GSH levels, and reduced MDA levels and SOD activity (p˂0.05), and decreased IL-1β (p˂0.05) and TNF-α (p˂0.01) levels. A significant reduction in walking distance, ambulation, speed, and rearing was observed for motor activity. Curcumin reduced oxidative stress, inflammation, and motor activity in hamsters with 5-FU-induced OM.

RESUMO Objetivo: Avaliar a estenose lombar degenerativa em pacientes sintomáticos submetidos à cirurgia, analisando a correlação entre fatores determinantes prováveis. Métodos: Estudo retrospectivo descritivo, no qual foram avaliados exames de ressonância nuclear magnética de 73 pacientes, totalizando 141 níveis com estenose degenerativa submetidos a tratamento cirúrgico por um único cirurgião do Hospital Ortopédico de Passo Fundo, de 2015 até 2018. Os pacientes foram estratificados de acordo com grau do tropismo facetário, artrose facetária, degeneração discal e lado operado, assim como com dados epidemiológicos como idade, sexo, etc. O tropismo foi aferido pelo método de Karacan e avaliado de forma numérica e categórica. A degeneração discal foi classificada por Pfirrmann e a artrose facetária por Weishaupt. Para análise e obtenção dos resultados foi utilizado o teste do qui-quadrado e ANOVA com oprograma estatístico SPSS versão 18.0. Resultados: Verificou-se significância estatística na relação entre tropismo facetário e degeneração discal (p=0,026) no nível L4-L5. Não foi encontrada correlação entre tropismo e artrose facetária (p=0,161) ou tropismo e lado operado(p=0,573). Conclusões: O grau de tropismo influencia diretamente a degeneração discal, sendo que as assimetrias maiores estão relacionadas a degenerações mais graves. Embora o tropismo não tenha demonstrado correlação estatística significativa com o lado operado (p=0,573), acredita-se que deverão ser realizados novos estudos sobre essa correlação. Nível de evidência II; Estudo retrospectivo.

ABSTRACT Objective: To evaluate degenerative lumbar stenosis in symptomatic patients submitted to surgeryand the correlation between probable determining factors. Methods: A retrospective descriptive study in which themagnetic resonance images of 73 patients with degenerative stenosis,who underwent surgeries involving 141 levels performed by a single surgeon at the Hospital Ortopédico de Passo Fundo from 2015 to 2018, were evaluated. The patients were stratified by the degree of facet tropism, facet arthrosis, disc degeneration, and operated side, as well as by epidemiological data such as age, sex, etc. Tropism was measured using the Karacan method and evaluated numerically and categorically. Disc degeneration was classified by Pfirrmann and facet arthrosis by Weishaupt. To analyze and obtain the results, the Chi-square test and ANOVA were used with the SPSS statistical program, version 18.0. Results: Statistical significance was found in the relationship between facet tropism and disc degeneration (p=0.026) at the L4-L5 level. No correlation was found between tropism and facet arthrosis (p=0.161) or tropism and the operated side (p=0.573). Conclusion: The degree of tropism directly influences disc degeneration and greater asymmetries are related to more severe degenerations. Although tropism has not shown a statistically significant correlation with the operated side (p=0.573), it is believed that further studies should be carried out on this correlation. Level of evidence II; Retrospective study.

RESUMEN Objetivo: Evaluar la estenosis lumbar degenerativa en pacientes sintomáticos sometidos a cirugía, analizando la correlación entre probables factores determinantes.. Métodos: Estudio retrospectivo descriptivo, en el que se evaluaronlos exámenes de resonancia magnética nuclear de 73 pacientes, totalizando 141 niveles con estenosis degenerativa sometidos a tratamiento quirúrgico por un único cirujano en el Hospital Ortopédico de Passo Fundo, de 2015 a 2018. Los pacientes fueron estratificados según elgrado de tropismo facetario, artrosis facetaria, degeneración discal y lado operado, así como con datos epidemiológicos como edad, sexo, etc. El tropismo se midió utilizando el método de Karacan y se evaluó numérica y categóricamente. La degeneración discal fue clasificada por Pfirrmann y la artrosis facetaria por Weishaupt. Para analizar y obtener los resultados se utilizó la prueba de chi-cuadrado y ANOVA a través del programa estadístico SPSS versión 18.0. Resultados: Se encontró significación estadística en la relación entre el tropismo facetario y la degeneración discal (p=0,026) en el nivel L4-L5. No se encontró correlación entre el tropismo y la artrosis facetaria (p=0,161) ni el tropismo y el lado operado (p=0,573). Conclusión: El grado de tropismo influye directamente en la degeneración discal, y las mayores asimetrías se relacionan con degeneraciones más severas. Aunque el tropismo no ha mostrado una correlación estadísticamente significativa con el lado operado (p=0,573), se cree que deberían realizarse más estudios sobre esta correlación. Nivel de evidencia II; Estudio retrospectivo.

ABSTRACT Objective To evaluate the incidence of variant hemoglobins of newborn samples from the Neonatal Screening Center in the state of Mato Grosso do Sul, Brazil, and to analyze the distribution and spatial autocorrelation of newborns with sickle cell trait. Methods Samples from 35,858 newborns screened by the Neonatal Screening Center. The samples with inconclusive diagnosis were submitted to electrophoretic, chromatographic, cytological and molecular analyses. The spatial distribution analysis of newborns with sickle cell trait was performed by spatial autocorrelation. Results A total of 919 newborns showed an abnormal hemoglobin profile; in that, ten genotypes had significant clinical impacts identified. Among the asymptomatic newborns, the sickle cell trait was the most frequent (incidence of 1.885 cases/100 newborns). The highest incidence rates were registered in the municipalities of Terenos, Figueirão, Corguinho and Selvíria. There was positive spatial autocorrelation between the proportion of declared individuals of black race/color and the incidence of newborns with sickle cell trait. Conclusion The early diagnosis by neonatal screening and laboratory tests was very important to identify abnormal hemoglobin profiles and guide the spatial autocorrelation analysis of sickle cell trait newborns in Mato Grosso do Sul, serving as a support to anticipate health measures aimed to discuss efficient therapeutic behaviors and effective planning of municipalities with the greatest need for care, monitoring and orientations for affected families.

RESUMO Introdução: Alterações na taxa de desenvolvimento de torque (TDT) e arquitetura muscular causadas pela fadiga são temas pouco investigados. Esta pesquisa avaliou o efeito da fadiga na TDT e arquitetura muscular do vasto lateral (VL). Métodos: Dezessete voluntários (25,5 ± 6,2 anos; 177,2 ± 12,9 cm; 76,4 ± 13,1 kg) realizaram avaliação isocinética de extensão do joelho em 30º/s para obtenção do pico de torque (PT-ISK) antes e após uma série de contrações voluntárias isométricas máximas (CVIM) intermitentes (15 reps - 3 s contração, 3 s intervalo) utilizadas para causar fadiga muscular, monitoradas pela frequência mediana (FMD) da eletromiografia do VL, reto femoral e vasto medial. Antes e após o protocolo de fadiga, imagens de ultrassonografia do VL foram obtidas para mensuração da espessura muscular, comprimento de fascículo e ângulo do fascículo. O pico de torque isométrico (PT-ISM) e TDTs em janelas de 50 ms foram calculados para cada CVIM. As TDTs foram calculadas em valores absolutos e normalizadas pelo PT-ISM. Resultados: A fadiga foi confirmada devido a reduções significativas da FMD dos três músculos. Após a fadiga, o PT-ISK foi reduzido de 239,0 ± 47,91 para 177,3 ± 34,96 Nm, e o PT-CVIM de 269,5 ± 45,63 para 220,49 ± 46,94 Nm. Todos os valores de TDT absolutos apresentaram-se significativamente diferentes após o protocolo de fadiga. Contudo, as TDTs normalizadas não demonstraram diferença significativa. Não foram observadas diferenças significativas na arquitetura muscular do VL. Conclusões: A redução da capacidade explosiva ocorreu de maneira concomitante com a redução da força máxima evidenciada pela falta de alterações na TDT normalizada. Nível de Evidência III.

ABSTRACT Introduction: There has been little research on changes in rate of torque development (RTD) and muscle architecture. This study evaluated the effect of fatigue on RTD and muscle architecture of the vastus lateralis (VL). Methods: Seventeen volunteers (25.5 ± 6.2 years; 177.2 ± 12.9 cm; 76.4 ± 13.1 kg) underwent isokinetic knee extension assessment at 30°/s to obtain the peak torque (PT-ISK), before and after a set of intermittent maximal voluntary isometric contractions (MVIC) (15 reps – 3 s contraction, 3 s rest) used to promote muscle fatigue, monitored by the median frequency (MDF) of the electromyography from the VL, rectus femoris and vastus medialis muscles. Before and after the fatigue protocol, ultrasound images of the VL were obtained to measure muscle thickness (MT), fascicle length (FL), and fascicle angle (FA). The peak isometric torque (PT-ISM) and the RTDs in 50 ms windows were calculated for each MVIC. The RTDs were reported as absolute values and normalized by the PT-ISM. Results: Fatigue was confirmed due to significant reductions in MDF in all three muscles. After the fatigue protocol, the PT-ISK was reduced from 239.0±47.91 to 177.3±34.96 Nm, and the PT-MVIC was reduced from 269.5±45.63 to 220.49±46.94 Nm. All the RTD absolute values presented significant change after the fatigue protocol. However, the normalized RTD did not demonstrate any significant differences. No significant differences were found in the muscle architecture of the VL. Conclusions: The reduction in explosive strength occurred concomitantly with the reduction in maximum strength, as evidenced by the lack of changes in normalized TDT. Level of Evidence III.

RESUMEN Introducción: Los cambios en la tasa de desarrollo del torque (TDT) y la arquitectura muscular causada por la fatiga son temas poco investigados. Esta investigación evaluó el efecto de la fatiga en la TDT y la arquitectura muscular del vasto lateral (VL). Métodos: 17 voluntarios (25.5 ± 6.2 años; 177.2 ± 12.9 cm; 76.4 ± 13.1 kg) se sometieron a una evaluación isocinética de la extensión de la rodilla a 30º/s para obtener el pico de torque (PT-ISK), antes y después de una serie de contracciones voluntarias máximas intermitentes (CVIM) (15 repeticiones - contracción 3 s, intervalo 3 s) utilizadas para causar fatiga muscular, monitoreadas por la frecuencia media (FMD) de la electromiografía VL, recto femoral y vasta medial. Antes y después del protocolo de fatiga, se obtuvieron imágenes de ultrasonido VL para medir el grosor muscular, la longitud del fascículo y el ángulo del fascículo. Se calculó el par isométrico máximo (PT-ISM) y las TDT en ventanas de 50 ms para cada CVIM. Las DTT se calcularon en valores absolutos y se normalizaron mediante PT-ISM. Resultados: la fatiga se confirmó por reducciones significativas en la fiebre aftosa de los tres músculos. Después de la fatiga, el PT-ISK se redujo de 239.0 ± 47.91 a 177.3 ± 34.96 Nm, y el PT-CVIM de 269.5 ± 45.63 a 220.49 ± 46.94 Nm. Todos los valores absolutos de TDT fueron significativamente diferentes después del protocolo de fatiga. Sin embargo, las TDT normalizadas no mostraron diferencias significativas. No se observaron diferencias significativas en la arquitectura muscular de la LV. Conclusiones: La reducción en la capacidad explosiva ocurrió simultáneamente con la reducción en la fuerza máxima, evidenciada por la falta de cambios en la TDT normalizada. Nível de evidencia III.

Abstract AISI D6 tool steel and AISI 304 stainless steel are among the most widely used material in the industry. However, this application field can still be expanded through the coatings deposition. In this regard, the objective of this work was to evaluate the impact of coatings application by cathodic cage technique using Hastelloy’s cathodic cage, on corrosion resistance of these steels. Two treatment temperatures were compared. The samples were characterized by Optical Microscopy, Scanning Electron Microscopy, Energy Dispersive Spectroscopy, X-Ray Diffraction with the application of the Rietveld routine and corrosion test. The results showed the formation of multiphase layers that contributed to corrosion potentials reduction for treatments at 400 °C, being more effective when applied on AISI D6. It was observed that the corrosion resistance is influenced by the temperature, which was attributed to the precipitation of chromium nitride and iron oxide at 450 ºC.

RESUMO Objetivo Avaliar o conhecimento de alunos dos cursos de enfermagem e medicina de uma universidade pública sobre a profilaxia pré e pós exposição ao HIV/Aids. Método estudo transversal, realizado com estudantes de enfermagem e medicina (n=167). Para explicar o efeito das variáveis sobre o conhecimento, utilizou-se a Regressão Logística Multinomial. Resultados Os participantes tinham em média 23,03 anos, sexo feminino (56,5%), solteiros (64,3%), heterossexuais (86,3%). O nível de conhecimento demonstrado foi majoritariamente médio (49,4%), e apenas 28,6% demonstraram um alto conhecimento acerca da temática. A análise multivariada mostrou que o curso (p=0,03) e a idade maior que 24 anos (p=0,01) foram associados a um maior nível de conhecimento. Conclusões Estudantes de enfermagem e medicina possuem conhecimento considerado como médio sobre as profilaxias preventivas ao HIV, cabendo assim às instituições de ensino em saúde fornecer subsídios para uma melhor formação dos estudantes, tratando o tema como assunto transversal em sua formação.

ABSTRACT Objective To evaluate students' knowledge of nursing and medical courses at a public university on prophylaxis before and after exposure to HIV/AIDS. Method cross-sectional study, carried out with nursing and medical students (n = 167). In order to explain the effect of variables on knowledge, the Multinomial Logistic Regression was used. Results Participants had a mean of 23.03 years, female (56.5%), unmarried (64.3%), and heterosexual (86.3%). The level of knowledge demonstrated was mostly medium (49.4%), and only 28.6% showed a high knowledge about the subject. The multivariate analysis showed that the course (p = 0.03) and age (p = 0.01) were associated with a higher level of knowledge. Conclusions Nursing and medical students possess knowledge considered as average about HIV preventive prophylaxis, thus it is up to the health education institutions to provide subsidies for a better training of students, treating the theme as a transversal subject in their training.

RESUMEN Objetivo Evaluar el conocimiento de alumnos de los cursos de enfermería y medicina de una universidad pública sobre la profilaxis pre y post exposición al VIH / SIDA. Método estudio transversal, realizado con estudiantes de enfermería y medicina (n = 167). Para explicar el efecto de las variables sobre el conocimiento, se utilizó la Regresión Logística Multinomial. Resultados Los participantes tenían en promedio 23,03 años, sexo femenino (56,5%), solteros (64,3%), y eran heterosexuales (86,3%). El nivel de conocimiento demostrado fue mayoritariamente medio (49,4%), y sólo el 28,6% demostró un alto conocimiento acerca de la temática. El análisis multivariado mostró que el curso (p = 0,03) y la edad (p = 0,01) se asociaron a un mayor nivel de conocimiento. Conclusiones Estudiantes de enfermería y medicina poseen conocimiento considerado como medio sobre las profilaxis preventivas al VIH, por lo tanto, corresponde a las instituciones de educación sanitaria proporcionar subsidios para una mejor capacitación de los estudiantes, tratando el tema como un tema transversal en su capacitación.