Pesquisa | SciELO

ABSTRACT Introduction: There is a lack of information about cardiac surgery training and professional practice in Latin American (LATAM) countries. This study is the first comparative analysis of cardiac surgical training and professional practice across LATAM and provides the fundamentals for future academic projects of the Latin American Association of Cardiac and Endovascular Surgery (LACES). Methods: International survey-based comparative analysis of the training and professional practice of cardiac surgeons across LATAM. Trainees (residents/fellows) and staf (graduated) surgeons from LATAM countries were included. Results: A total of 289 respondents (staf surgeons N=221 [76.5%]; residents/fellows N=68 [23.5%]) from 18 different countries participated in the survey. Most surgeons (N=92 [45.3%]) reported being unsatisfied with their salaries. Most respondents (N=181 [62.6%]) stated that it was difficult to obtain a leadership position, and 149 (73.8%) stated that it was difficult to find a job after completing training. Only half of the trainee respondents (N=32 [47.1%]) reported that their program had all resident spots occupied. Only 22.1% (N=15) of residents/fellows were satisfied with their training programs. The majority (N=205 [70.9%]) of respondents would choose cardiac surgery as their specialty again. Most surgeons (N=129 [63.9%]) and residents/fellows (N=52 [76.5%]) indicated that the establishment of a LATAM cardiac surgery board examination would be beneficial. Conclusion: Modernization and standardization of training, as well as greater access to opportunities, may be required in LATAM to increase professional satisfaction of cardiac surgeons and to reduce disparities in the specialty. Such changes may enhance the regional response to the dynamic challenges in the feld. Introduction (LATAM LACES. LACES . (LACES) Methods surveybased survey based residentsfellows residents fellows (residents/fellows graduated (graduated included Results 28 N221 N 221 N=22 76.5% 765 76 5 [76.5%] N68 68 N=6 23.5% 235 23 [23.5%] 1 N=92 N92 92 (N=9 45.3% 453 45 3 [45.3%] salaries N=181 N181 181 (N=18 62.6% 626 62 6 [62.6%] position 14 73.8% 738 73 8 (73.8% N=32 N32 32 (N=3 47.1% 471 47 [47.1%] occupied 22 22.1 N=15 N15 15 (N=15 programs N=205 N205 205 (N=20 70.9% 709 70 9 [70.9%] again N=129 N129 129 (N=12 63.9% 639 63 [63.9%] N=52 N52 52 (N=5 beneficial Conclusion opportunities feld (LACES 2 N22 N=2 76.5 7 [76.5% N6 N= 23.5 [23.5% N=9 N9 (N= 45.3 4 [45.3% N=18 N18 (N=1 62.6 [62.6% 73.8 (73.8 N=3 N3 47.1 [47.1% 22. N=1 N1 N=20 N20 20 (N=2 70.9 [70.9% N=12 N12 12 63.9 [63.9% N=5 N5 N2 76. [76.5 23. [23.5 (N 45. [45.3 62. [62.6 73. (73. 47. [47.1 70. [70.9 63. [63.9 [76. [23. [45. [62. (73 [47. [70. [63. [76 [23 [45 [62 (7 [47 [70 [63 [7 [2 [4 [6 ( [

https://doi.org/10.21470/1678-9741-2022-0125

Diretriz Brasileira de Dispositivos Cardíacos Eletrônicos Implantáveis – 2023 202 20 2
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Teixeira, Ricardo Alkmim

; Fagundes, Alexsandro Alves

; Baggio Junior, José Mário

; Oliveira, Júlio César de

; Medeiros, Paulo de Tarso Jorge

; Valdigem, Bruno Pereira

; Teno, Luiz Antônio Castilho

; Silva, Rodrigo Tavares

; Melo, Celso Salgado de

; Elias Neto, Jorge

; Moraes Júnior, Antonio Vitor

; Pedrosa, Anisio Alexandre Andrade

; Porto, Fernando Mello

; Brito Júnior, Hélio Lima de

; Souza, Thiago Gonçalves Schroder e

; Mateos, José Carlos Pachón

; Moraes, Luis Gustavo Belo de

; Forno, Alexander Romeno Janner Dal

; D’Avila, Andre Luiz Buchele

; Cavaco, Diogo Alberto de Magalhães

; Kuniyoshi, Ricardo Ryoshim

; Pimentel, Mauricio

; Camanho, Luiz Eduardo Montenegro

; Saad, Eduardo Benchimol

; Zimerman, Leandro Ioschpe

; Oliveira, Eduardo Bartholomay

; Scanavacca, Mauricio Ibrahim

; Martinelli Filho, Martino

; Lima, Carlos Eduardo Batista de

; Peixoto, Giselle de Lima

; Darrieux, Francisco Carlos da Costa

; Duarte, Jussara de Oliveira Pinheiro

; Galvão Filho, Silas dos Santos

; Costa, Eduardo Rodrigues Bento

; Mateo, Enrique Indalécio Pachón

; Melo, Sissy Lara De

; Rodrigues, Thiago da Rocha

; Rocha, Eduardo Arrais

; Hachul, Denise Tessariol

; Lorga Filho, Adalberto Menezes

; Nishioka, Silvana Angelina D’Orio

; Gadelha, Eduardo Barreto

; Costa, Roberto

; Andrade, Veridiana Silva de

; Torres, Gustavo Gomes

; Oliveira Neto, Nestor Rodrigues de

; Lucchese, Fernando Antonio

; Murad, Henrique

; Wanderley Neto, José

; Brofman, Paulo Roberto Slud

; Almeida, Rui M. S.

; Leal, João Carlos Ferreira

Arquivos Brasileiros de Cardiologia

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2023, Volume 120 Nº 1 elocation e20220892

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

https://doi.org/10.36660/abc.20220892

INVESTIGACIONES ARQUEOLÓGICAS EN EL CENTRO DE LA PROVINCIA DE CORRIENTES: PRIMEROS RESULTADOS DE LAS PROSPECCIONES EN EL SISTEMA IBERÁ
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Píccoli, Carolina V.

; Barboza, Carolina

; Contreras, Félix I.

; Fernández López, Carolina

; Rey Montoya, Tania S.

; Saucedo, Omar N.

; Cuaranta, Pedro

; Monferran, Mateo D.

; Gallego, Oscar F.

; Mujica, Juan I.

Folia Histórica del Nordeste

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2023, Nº 46 Páginas 241 - 268

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen: Las investigaciones arqueológicas en el centro de la provincia de Corrientes han sido escasas y discontinuas. Con el objetivo de revertir este panorama se iniciaron trabajos de campo en el sector sur y sur-sureste del macrosistema Iberá (Reserva Provincial Iberá y Parque Nacional Iberá). En este trabajo se presentan los resultados de las actividades de prospección y excavación desarrolladas. Como resultado de las mismas, se identificaron 11 áreas en las que los materiales arqueológicos se presentan en densidades variables y asociados a una matriz sedimentaria con alta proporción de conchillas. En líneas generales, estas se caracterizan por la presencia de material cerámico, lítico, restos óseos humanos y faunísticos, ornamentos, entre otros. A esta información, se integran y discuten los antecedentes para el sector, confirmando el alto potencial arqueológico del área. La evaluación de la red de permeabilidad del sector permitió identificar tres formas posibles de desplazamiento práctico entre la totalidad de los sitios hasta ahora identificados (n= 33).

Abstract Archaeological research in the center of Corrientes province has been scarce. This paper presents the results of the archeological survey carried out in the south and southeast of the Iberá wetlands (Iberá Reserve and Iberá National Park). Eleven archaeological sites were recorded, with different density values of pottery, lithics, animal bones, human remains, and ornaments. The stratigraphic matrices of most of them are composed in general by shells. There are differences regarding soil organic carbon and soil phosphorus among the sites; pH and electrical conductivity remain neutral. The new information and older survey data confirm the high archaeological potential of this area. Three possible main path of movement were identified among the recorded sites (n=33).

https://doi.org/10.30972/fhn.4606502

COVID-19 and Cardiovascular Surgery. Do We Know What We Are Dealing With?
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Almeida, Rui M. S.

; Marin-Cuartas, Mateo

; Garcia-Villarreal, Ovidio A.

; Dayan, Victor

Brazilian Journal of Cardiovascular Surgery

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Dez 2021, Volume 36 Nº 6 Páginas III - IV

Resumo: En | Texto: En | PDF: En

https://doi.org/10.21470/1678-9741-2021-0962

The official position of the Latin American Association of Cardiac and Endovascular Surgery (LACES) regarding the recently released SOLACI/SIAC Clinical Guidelines on TAVI versus SAVR LACES (LACES SOLACISIAC SOLACI SIAC
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Dayan, Victor

; Garcia-Villarreal, Ovidio A.

; Escobar, Alejandro

; Ferrari, Javier

; Quintana, Eduard

; Marin-Cuartas, Mateo

; Almeida, Rui M. S.

Brazilian Journal of Cardiovascular Surgery

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Ago 2021, Volume 36 Nº 4 Páginas 584 - 586

Resumo: En | Texto: En | PDF: En

https://doi.org/10.21470/1678-9741-2021-0385 18 downloads

Resistencia a Sporisorium reilianum f. sp. zeae en germoplasma nativo de maíz
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Márquez-Licona, Guillermo

; Castillo-González, Fernando

; León-García de Alba, Carlos De

; Vargas-Hernández, Mateo

; Solano-Báez, Alma Rosa

; Leyva-Mir, Santos Gerardo

; Téliz-Ortíz, Daniel

Revista mexicana de fitopatología

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2021, Volume 39 Nº 1 Páginas 1 - 20

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Head smut of maize (Sporisorium reilianum f. sp. zeae) is a disease characterized by the pathogen replacing inflorescences with sori full of teliospores. The objective of this study was to investigate the response of 55 native maize populations to S. reilianum infection. Maize populations were collected in the states of Guerrero (13), Oaxaca (13), Puebla (six), Tlaxcala (12) and Estado de México (11). The seed was inoculated with teliospores of the pathogen, using grenetine as adherent. The hybrid Az 41801 was used as a control. The populations were evaluated in Mixquiahuala, Hidalgo, in the 2015 and 2016 plantings. The incidence of the disease was recorded by direct observation of signs and symptoms in the inflorescences. The maximum incidence of the disease in the maize populations was 28.8% and 22.2% in the first and second evaluation, respectively, while the control (Az 41801) presented an incidence of 70.7% and 42.3%. Considering the geographical origin, the native maize collections from the Estados de Mexico and Tlaxcala, had a lower incidence of the disease compared to the rest of the populations, which indicates the presence of genes for resistance to the disease.

Resumen El carbón de la espiga del maíz (Sporisorium reilianum f. sp. zeae) es una enfermedad que se caracteriza porque el patógeno remplaza las inflorescencias por soros llenos de teliosporas. El objetivo de este estudio fue investigar la respuesta en campo de 55 poblaciones de maíz nativo a la infección por S. reilianum. Las poblaciones de maíz se colectaron en los estados de Guerrero (13), Oaxaca (13), Puebla (seis), Tlaxcala (12) y Estado de México (11). La semilla fue inoculada con teliosporas del patógeno, usando grenetina como adherente. El híbrido Az 41801 se utilizó como testigo. Las poblaciones fueron evaluadas en Mixquiahuala, Hidalgo, en los ciclos 2015 y 2016. La incidencia de la enfermedad se registró mediante la observación directa de signos y síntomas en las inflorescencias. La incidencia máxima de la enfermedad en las poblaciones de maíz fue de 28.8% y 22.2% en la primera y segunda evaluación, respectivamente, mientras que el testigo (Az 41801) presentó una incidencia de 70.7% y 42.3%. Considerando el origen geográfico, los maíces del Estado de México y Tlaxcala presentaron una menor incidencia de la enfermedad respecto al resto de las poblaciones, lo que indica la presencia de genes de resistencia a la enfermedad.

https://doi.org/10.18781/r.mex.fit.2005-5 393 downloads

Long-term fertilization with dairy cattle slurry in intensive production systems: effects on soil porosity and pore morphology
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Valdez-Ibañez, Alcira S.

; Domingo-Olivé, Francesc

; Mateo-Marín, Noemí

; Yagüe-Carrasco, María R.

; Bosch-Serra, Ángela D.

Revista de la Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo

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Dez 2019, Volume 51 Nº 2 Páginas 248 - 260

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

In Mediterranean environments, livestock effluents might improve soil physical properties. The study was located in an intensive crop production system of northwest Spain. After nine consecutive years of dairy cattle slurry (DCS) use as fertilizer, the aim of the experiment was to evaluate the impacts of DCS on soil porosity and pore shape. Soil texture was loam. The applied DCS rates were equivalent to 170 and 250 kg N ha-1 (170DCS and 250DCS, respectively) and they were complemented with mineral N up to 450 kg N ha-1 (two crops). A nonfertilized control was included. Digital binary images were obtained from soil thin sections. Pores with an apparent diameter (AD) >30 μm were analysed. The 250DCS treatment improved soil porosity (>30 μm): it doubled in comparison with the 170DCS and the control. The application of DCS favored the presence of pores with an AD >400 μm, the roughness for AD >100 μm and the elongation in the AD interval of 100-200 μm. From the study, the 250DCS treatment is recommended as it increases macroporosity (compaction reduction) and produces more elongated and tortuous pores, which will be a constraint for fast drainage but it will be advantageous in coarse textured soils.

En zonas Mediterráneas del noroeste español bajo producción intensiva, la aplicación de deyecciones ganaderas podría mejorar las propiedades físicas del suelo. Tras nueve años de fertilización con purín de vacas lecheras (DCS), se planteó evaluar los efectos del DCS sobre la porosidad edáfica y la morfología de los poros. La clase de familia textural era franca gruesa. Las dos dosis de DCS evaluadas equivalían a 170 y 250 kg N ha-1(170DCS y 250DCS, respectivamente) y se complementaban con N mineral hasta 450 kg N ha-1 (dos cultivos). Se incluyó un control, sin fertilización. A partir de láminas delgadas de suelo se obtuvieron imágenes digitales binarizadas. Se analizaron los poros de diámetro aparente (AD) >30 μm. El tratamiento 250DCS duplicó la porosidad (>30 μm) respecto a 170DCS y el control. La aplicación de DCS favoreció la presencia de AD >400 μm, la rugosidad para AD >100 μm y la elongación de poros en el rango 100-200 μm. La dosis de 250DCS es recomendable al incrementar la macroporosidad (implica una disminución de la compactación) y favorecer unas formas de poros más alargadas, con mayor tortuosidad, que dificultarían el drenaje rápido, lo cual es beneficioso en suelos con contenido importante de arena.

195 downloads

Impactos del cambio climático sobre la flora acuática de las Lagunas Verdes, volcán Chiles, Ecuador
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Terneus Jácome, Esteban

; Vallejo, Berenice

; Gómez de la Torre, Mateo

Biota colombiana

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Dez 2019, Volume 20 Nº 2 Páginas 20 - 31

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen Evaluamos los impactos del cambio climático sobre la estructura y composición florística de las plantas acuáticas en cinco lagunas altoandinas (3900 m s. n. m.), al pie del volcán Chiles. Utilizamos transectos y cuadrantes de profundidad para evaluar riqueza y patrones de cobertura vegetal, y evaluamos la físicoquímica de agua con equipos electrónicos de medición in situ. Esta información fue comparada con la generada hace 17 años. Registramos 15 especies de plantas acuáticas, de las cuales se evidenció reducción en las poblaciones de Isoetes sp., Crassula venezuelensis y Elatine ecuadoriensis. Al parecer, Lilaeopsis schaffneriana y Juncus stipulatus son remplazadas por Lachemilla nivalis, y una Poaceae del género Agrostis como colonizadora. Las características abióticas de las lagunas evidencian cambios en el pH y la conductividad de sus aguas, generando condiciones particulares para un posible establecimiento de especies distintas a los registros históricos. Evidenciamos perjuicios sobre la naturaleza del ecosistema acuático, por la pérdida o reemplazo de especies hidrófitas por helófitas, como indicadoras de un potencial proceso de transformación de ambientes naturales, y un inminente riesgo de la permanencia y la funcionalidad ecológica de los ecosistemas acuáticos del lugar.

Abstract We evaluated the impact of climate change on the structure and floral composition of the aquatic plants occurring in five Andean lakes (3900 m a. s. l.) at the foot of the Chiles Volcano. We obtained field information using transects and depth quadrants to evaluate richness and cover vegetation patterns, whereas physical chemistry of water was evaluated using electronic measuring equipment in situ. Part of the information from the same lakes 17 years ago was used as a basis for comparison. Were recorded fifteen species of aquatic plants, with evidence of a reduction in the populations of Isoetes sp., Crassula venezuelensis and Elatine ecuadoriensis. Apparently, Lilaeopsis schaffneriana and Juncus stipulatus were replaced by Lachemilla nivalis and a Poaceae of the genus Agrostis as a colonizer. The abiotic characteristics of the lakes show changing trends in pH and conductivity of the waters, turning them more neutral than acidic. This leads to conditions that may allow for the establishment of species other than those of historical records, increasing the general variety, implying the possibility that climate change effects are not necessarily negative in this situation. However, there is evidence of damage to the natural aquatic ecosystem, through the loss or replacement of hydrophilic for halophile plants. This potential transformation process of the natural environment jeopardizes the ecological functioning of the aquatic ecosystems in the area.

https://doi.org/10.21068/c2019.v20n02a02 689 downloads

Bloqueo más radiofrecuencia térmica de los nervios esplácnicos. A propósito de un caso
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López-Palanca, S.

; Montalbán-Moreno, B.

; González-López, T.

; Mateo-Cerdán, C.

; Cuartero-Pozo, A.-B.

; Arcas-Molina, M.

Revista de la Sociedad Española del Dolor

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Out 2018, Volume 25 Nº 5 Páginas 303 - 304

Resumo: Es | Texto: Es | PDF: Es

https://doi.org/10.20986/resed.2016.3509/2016 465 downloads

10.

Efectividad del parche de capsaicina 8% en el dolor neuropático
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Montalbán-Moreno, B.

; González-López, M.T.

; Mateo-Cerdán, C.M.

; López-Palanca, S.

; Jiménez-Jiménez, V.

; Cuartero-del-Pozo, A.B.

; Garvi-López, M.

; Arcas-Molina, M.Z.

Revista de la Sociedad Española del Dolor

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Ago 2018, Volume 25 Nº 4 Páginas 242 - 243

Resumo: Es | Texto: Es | PDF: Es

https://doi.org/10.20986/resed.2017.3536/2016 505 downloads

11.

Palmas útiles en tres comunidades indígenas de La Pedrera, Amazonia colombiana
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JAIMES-RONCANCIO, MATEO S.

; BETANCUR, JULIO

; CÁMARA-LERET, RODRIGO

Caldasia

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Jun 2018, Volume 40 Nº 1 Páginas 112 - 128

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

RESUMEN Se estudió el conocimiento y el uso de las palmas por parte de las comunidades indígenas Angostura, Curare y Yukuna, cercanas a la cabecera del corregimiento de La Pedrera, Amazonia colombiana. Entre junio y julio de 2010 se registró información etnobotánica mediante entrevistas estructuradas realizadas a 53 personas informantes de dichas comunidades. Los datos se analizaron aplicando los índices de importancia relativa de categoría de uso (IR(c)), importancia cultural relativa (IC(s)) e importancia relativa de cada especie (IR(s)). Se encontraron 34 especies de palmas útiles y nueve categorías de uso. Según el IR(c) las categorías de uso más importantes fueron Alimentación humana, Construcción y Utensilios y herramientas. Dentro de estas se destaca el consumo directo de los frutos, la elaboración de bebidas, la utilización de la palma entera o de las hojas para la construcción de viviendas y la elaboración de instrumentos de caza y utensilios para el uso cotidiano. Según el IR(s) e IC(s) las especies más importantes fueron el chontaduro (Bactris gasipaes), el canangucho (Mauritia flexuosa), el milpesos (Oenocarpus bataua) y el asaí (Euterpe precatoria). Se confirma que las palmas juegan un papel importante en las tres comunidades y se evidencia una disminución del conocimiento en los usos asociados a la cultura y a las ceremonias rituales. Por otra parte, según los índices de importancia relativa y cultural coinciden las mismas especies de palmas registradas en otras comunidades amazónicas colombianas.

ABSTRACT We studied the knowledge and use of palms in three indigenous communities (Angostura, Curare and Yukuna) near the corregimiento of La Pedrera, Colombian Amazonia. Between June and July of 2010 ethnobotanical data was recorded through structured interviews with 53 informants. The data was analyzed using three indices: relative importance of use category (IR (c)), relative cultural importance (IC (s)) and relative species importance (IR (s)). We found 34 useful palms species, used in nine different use categories. According to the IR (c) the most important use categories were Human Food, Construction and Utensils and tools. Notable among these were the direct consumption of fruits, preparation of beverages, the use of the entire palm and the leaves for housing, and the manufacture of weapons for hunting and utensils for daily use. According to the IR (s) and IC (s) the most important species were chontaduro (Bactris gasipaes), canangucho (Mauritia flexuosa), milpesos (Oenocarpus bataua), and asaí (Euterpe precatoria). Palms play an important role in all three communities, but there is a notable decrease in knowledge about uses associated with culture and ritual ceremonies. According to the indices or relative cultural and relative species importance, the species that were important in this study resembled those found in other Colombian Amazon communities.

https://doi.org/10.15446/caldasia.v40n1.68851 826 downloads

12.

3rd Guideline for Perioperative Cardiovascular Evaluation of the Brazilian Society of Cardiology
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Gualandro, Danielle Menosi

; Yu, Pai Ching

; Caramelli, Bruno

; Marques, André Coelho

; Calderaro, Daniela

; Fornari, Luciana Savoy

; Pinho, Claudio

; Feitosa, Alina Coutinho Rodrigues

; Polanczyk, Carisi Anne

; Rochitte, Carlos Eduardo

; Jardim, Carlos

; Vieira, Carolina L. Z.

; Nakamura, Debora Y. M.

; Iezzi, Denise

; Schreen, Dirk

; Adam, Eduardo Leal

; D’Amico, Elbio Antonio

; Lima, Emerson Q. de

; Burdmann, Emmanuel de Almeida

; Mateo, Enrique Indalecio Pachón

; Marcondes-Braga, Fabiana Goulart

; Machado, Fabio S.

; Paula, Flavio J. de

; Carmo, Gabriel Assis Lopes do

; Feitosa-Filho, Gilson Soares

; Prado, Gustavo Faibischew

; Lopes, Heno Ferreira

; Fernandes, João R. C.

; Lima, José J. G. de

; Sacilotto, Luciana

; Drager, Luciano Ferreira

; Vacanti, Luciano Janussi

; Rohde, Luis Eduardo Paim

; Prada, Luis F. L.

; Gowdak, Luis Henrique Wolff

; Vieira, Marcelo Luiz Campos

; Monachini, Maristela Camargo

; Macatrão-Costa, Milena Frota

; Paixão, Milena Ribeiro

; Oliveira Junior, Mucio Tavares de

; Cury, Patricia

; Villaça, Paula R.

; Farsky, Pedro Silvio

; Siciliano, Rinaldo F.

; Heinisch, Roberto Henrique

; Souza, Rogerio

; Gualandro, Sandra F.M.

; Accorsi, Tarso Augusto Duenhas

; Mathias Júnior, Wilson

Arquivos Brasileiros de Cardiologia

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Set 2017, Volume 109 Nº 3 Páginas 1 - 104

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

https://doi.org/10.5935/abc.20170140 100302 downloads

13.

Stenotrophomonas maltophilia isolated from gasoline-contaminated soil is capable of degrading methyl tert-butyl ether
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Alfonso-Gordillo, Guadalupe

; Cristiani-Urbina, Eliseo

; Flores-Ortiz, César Mateo

; Peralta, Humberto

; Cancino-Díaz, Juan Carlos

; Cruz-Maya, Juan Antonio

; Jan-Roblero, Janet

Electronic Journal of Biotechnology

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Set 2016, Volume 19 Nº 5 Páginas 12 - 20

Resumo: En | Texto: En | PDF: En

Background: Methyl tert-butyl ether (MTBE) is a pollutant that causes deleterious effects on human and environmental health. Certain microbial cultures have shown the ability to degrade MTBE, suggesting that a novel bacterial species capable of degrading MTBE could be recovered. The goal of this study was to isolate, identify and characterize the members of a bacterial consortium capable of degrading MTBE. Results: The IPN-120526 bacterial consortium was obtained through batch enrichment using MTBE as the sole carbon and energy source. The cultivable fraction of the consortium was identified; of the isolates, only Stenotrophomonas maltophilia IPN-TD and Sphingopyxis sp. IPN-TE were capable of degrading MTBE. To the best of our knowledge, this report is the first demonstrating that S. maltophilia and Sphingopyxis sp. are capable of degrading MTBE. The degradation kinetics of MTBE demonstrated that S. maltophilia IPN-TD had a significantly higher overall MTBE degradation efficiency and rate (48.39 ± 3.18% and 1.56 ± 0.12 mg L-1 h-1, respectively) than the IPN-120526 consortium (38.59 ± 2.17% and 1.25 ± 0.087 mg L-1 respectively). The kinetics of MTBE removal by both cultures fit first-order and pseudo-first-order reaction models. Conclusions: These findings suggest that S. maltophilia IPN-TD in axenic culture has considerable potential for the detoxification of MTBE-contaminated water.

https://doi.org/10.1016/j.ejbt.2016.06.006 551 downloads

14.

Cerebrospinal fluid biomarkers for the differential diagnosis of Alzheimer’s disease
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Magalhães, Carolina A.

; Figueiró, Micheli

; Fraga, Vanessa G.

; Mateo, Elvis C.

; Toledo, André A. S. F.

; Carvalho, Maria das Graças

; Caramelli, Paulo

; Gomes, Karina B.

Jornal Brasileiro de Patologia e Medicina Laboratorial

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Dez 2015, Volume 51 Nº 6 Páginas 376 - 382

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Introdução: Estudos têm sido conduzidos no sentido de validar biomarcadores no liquor para o diagnóstico da doença de Alzheimer (DA), objetivando, sobretudo, facilitar o diagnóstico precoce. Objetivo: Avaliar os biomarcadores do liquor em indivíduos com provável DA, bem como a aplicabilidade dos valores de referência internacionais nesta população. Métodos: Foram recrutados 46 indivíduos, sendo classificados como provável DA (n = 19), comprometimento cognitivo leve (CCL) (n = 5) e outras demências (n = 22). Os biomarcadores foram dosados no liquor utilizando-se os kits INNOTEST por ensaio imunossorvente ligado à enzima (ELISA). Maiores valores de proteína Tau e menores valores de Aβ e índice Innotest Amiloide Tau Index (IATI) foram observados no grupo de DA quando comparados com o de CCL; maiores níveis de Tau e Tau fosforilada (Tau-P) e menores valores de Aβ e IATI foram observados no grupo de DA quando comparados com os pacientes que apresentavam outras demências. Nenhum biomarcador ou o IATI foi capaz de discernir entre CCL e outras demências. O índice kappa entre os biomarcadores e o diagnóstico clínico foi regular para a Tau e IATI, e fraco para Aβ e Tau-P. Conclusão: Os valores de cut-off para cada biomarcador que apresentou melhor sensibilidade e especificidade conjugadas diferiram dos valores de referência sugeridos pelo fabricante. Os biomarcadores do liquor representam importantes recursos que podem auxiliar no diagnóstico da DA, mas a interpretação dos resultados deve ser feita com base na análise dos três analitos em conjunto. Os valores de cut-off devem ser estabelecidos de modo a atender as especificidades e as características de cada população.

ABSTRACT Introduction: Several studies have been conducted in order to validate cerebrospinal fluid biomarkers for the diagnosis of Alzheimer’s disease (AD), aiming primarily to facilitate the early diagnosis. Objective: To evaluate CSF biomarkers on patients with probable AD and the applicability of the international references values in this population. Methods: 46 individuals were recruited and classified as probable AD (n = 19), mild cognitive impairment (MCI) (n = 5) and other dementias (n = 22). The cerebrospinal fluid (CSF) biomarkers were measured using the INNOTEST kits for enzyme-linked immunosorbent assay (ELISA). Higher Tau protein values and lower Aβand Innotest Amyloid Tau Index (IATI) values were observed in AD group when compared with MCI; higher levels of Tau and phosphorylated Tau (P-Tau), and lower Aβand IATI values were observed in AD group when compared to patients with other dementias. No biomarker or IATI was able to distinguish between MCI and other dementias. The kappa index between biomarkers and the clinical diagnosis was regular to Tau and IATI, and weak to Aβand P-tau. Conclusion: The cut-off values for each biomarker that showed better combined sensibility and specificity differ from the reference values suggested by the manufacturer. The CSF biomarkers represent important resources that can help with the AD diagnosis, although the results interpretation must be made based on the analysis of the three analytes together. The cut-off values must be established to address the specificities and characteristics of each population.

https://doi.org/10.5935/1676-2444.20150059 2997 downloads

15.

Simultaneous Determination of Catecholamines and Metanephrines in Urine by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry: Successful Clinical Application
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Diniz, Maria E. R.

; Vilhena, Leandro S.

; Paulo, Breno P.

; Barbosa, Tatiana C. C.

; Mateo, Elvis C.

Journal of the Brazilian Chemical Society

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Ago 2015, Volume 26 Nº 8 Páginas 1684 - 1691

Resumo: En | Texto: En | PDF: En

<p>Determination of urinary catecholamines and metanephrines is important for diagnosis of pheochromocytoma. Liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed for the determination of catecholamines and metanephrines in human urine using deuterated internal standards (IS). In this method, 1000 µL of urine samples were treated by liquid-liquid extraction using ethyl acetate and subjected to LC-MS/MS analysis using positive electrospray ionization (ESI+). A BDS HYPERSILTM C18 column (125 mm × 3 mm, 3 µm) was used and mobile phase was water:methanol (98:2, v/v) with 0.25% of formic acid at 200 µ L min<sup>−1</sup>. The method has a chromatographic running time of approximately 10 min. The average of recovery was 92.9-106.1% for epinephrine, 94.6-107.7% for norepinephrine, 98.0-108.8% the dopamine, 94.2-105.1% the metanephrine, 97.0-106.0% for normetanephrine. The precision was lower than 7.0% for all analytes. Furthermore, this method has been implemented successfully in routine laboratory due to its easy execution and excellent precision.</p>

https://doi.org/10.5935/0103-5053.20150142 5792 downloads

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