Abstract Objective To compare the effectiveness of inhaled Magnesium Sulfate associated with Salbutamol versus Inhaled Salbutamol alone in patients with moderate and severe asthma exacerbations. Method Clinical, prospective and randomized study with patients between 3 and 14 years of age divided into two groups: one to receive inhaled salbutamol associated with magnesium sulfate (GSM), the other to receive inhaled salbutamol alone (GS). The sample consisted of 40 patients, 20 patients in each group. Severity was classified using the modified Wood-Downes score, with values between 4 and 7 classified as moderate and 8 or more classified as severe. Results Post-inhalation scores decreased both in patients who received salbutamol and magnesium and in those who received salbutamol alone, with no statistically significant difference between the groups. Conclusions Despite the benefits when administered intravenously, inhalation of the drug alone or in combination did not reduce the severity of the exacerbation. exacerbations Clinical 1 groups GSM, GSM , (GSM) GS. GS . (GS) 2 group WoodDownes Wood Downes score Postinhalation Post intravenously exacerbation (GSM (GS

Abstract Introduction The World Health Organization (WHO) estimates that ~ 32 million children worldwide are affected by hearing loss (HL). Cochlear implant is the first-line treatment for severe to profound sensorineural HL. It is considered one of the most successful prostheses developed to date. Objective To evaluate the oral language development of pediatric patients with prelingual deafness implanted in a reference hospital for the treatment of HL in southern Brazil. Methods We conducted a retrospective cohort study with a review of medical records of patients undergoing cochlear implant surgery between January 2009 and December 2018. Language development was assessed by reviewing consultations with speech therapy professionals from the cochlear implant group. Results A total of 152 children were included in the study. The mean age at cochlear implant surgery was of 41 months (standard deviation [SD]: ± 15). The patients were divided into six groups considering the type of language most used in their daily lives. We found that 36% of children use oral language as their primary form of communication. In a subanalysis, we observed that patients with developed or developing oral language had undergone cochlear implant surgery earlier than patients using Brazilian Sign Language (Língua Brasileira de Sinais, LIBRAS, in Portuguese) or those without developed language. Conclusion The cochlear implant is a state-of-the-art technology that enables the re-establishment of the sense of hearing and the development of oral language. However, language development is a complex process known to present a critical period to properly occur. We still see many patients receiving late diagnosis and treatment, which implies a delay and, often, the impossibility of developing oral communication. Level of Evidence Level 3 (cohort study). WHO (WHO . (HL) firstline first line date Brazil 200 2018 group 15 4 standard SD [SD] 15. 15) lives 36 communication subanalysis Língua Sinais LIBRAS Portuguese stateoftheart state art reestablishment re establishment However occur often study) (HL 20 201 1 [SD 2

Resumo O desenvolvimento humano é influenciado pela interação entre fatores biológicos e sociais. Este estudo teve como objetivo verificar o efeito moderador do risco social na relação entre risco biológico e desenvolvimento infantil. Os dados foram coletados em 201 crianças, com idades entre 6 e 72 meses. A variável independente foi medida pelo índice de risco biológico, e a variável moderadora pelo índice de risco social. O desenvolvimento infantil foi avaliado por meio do teste Denver II. Regressão linear, tamanho do efeito e análise de moderação foram utilizados para verificar a relação entre IRB e o desenvolvimento infantil (Denver II) e o efeito moderador do IRS. O IRB associou-se negativamente ao desenvolvimento infantil, a interação entre o IRB e o IRS aumentou a variância explicada no resultado do Denver II para 14%. O IRS também foi um moderador significativo dos domínios Linguagem e Motor Grosso. Esta pesquisa evidenciou que o risco social é um moderador da relação entre risco biológico e desenvolvimento infantil; quanto mais fatores de risco social, mais forte essa relação se torna. Por outro lado, pode-se dizer que alguns fatores sociais favorecem o desenvolvimento infantil, mesmo na presença de fatores de risco biológicos. 20 crianças 7 meses linear associouse associou 14 14% Grosso torna lado podese pode 2 1

Abstract Human development is influenced by the interaction between biological and social factors. This study aimed to verify the moderating effect of social risk on the relationship between biological risk and child development. Data were collected on 201 children, aged 6 to 72 months. The independent variable was measured by the biological risk index, and the moderator variable by the social risk index was assessed by the Denver II test. Linear regression, effect size, and analysis of moderation were used to verify the relationship between BRI and the child development (Denver II), and the moderating effect of the SRI. BRI was negatively associated with child development, the interaction between the BRI and SRI increased the explained variance in the Denver II result to 14%. The SRI was also a significant moderator of the Language and Gross Motor domains. This research evidence that social risk moderates the relationship between biological risk and child development, the more social risk factors, the stronger this relationship becomes. On the other hand, it can be said that some social factors favor child development, even in the presence of biological risk factors. 20 children 7 months test regression size II, , II) 14 14% domains becomes hand 2 1

Abstract Objective: To assess the prevalence of chronic neutropenia (CN) and the clinical profile of patients with CN aged up to 18 years, followed in the pediatric hematology, rheumatology, or immunology outpatient clinic of a tertiary medical center from May 1, 2018, to 30 April 2019. Methods: Retrospective observational study carried out by collecting data from the patient’s medical charts. CN was defined as absolute neutrophil count (ANC) below 1.5 × 109/L lasting over three months. Autoimmune neutropenia (AIN) was defined by clinical criteria and an over twofold increase in ANC after glucocorticoid stimulation. AIN was considered secondary when associated with autoimmune or immunoregulatory disorders. Wilcoxon and Fisher’s exact tests were used to compare variables; the significance level was 5 %. Results: A total of 1,039 patients were evaluated; 217 (20 %) presented CN. Twenty-one (2 %) had AIN, classified as primary in 57 % of the cases. The average age at the onset of symptoms was 38.6 months. During follow-up, patients had 4.2 infections on average; frequency was higher among patients with secondary AIN (p = 003). Isolated neutropenia occurred in 43 % of the patients with AIN. Neutropenia resolved in eight (38 %) of the 21 patients with AIN within 19.6 months on average. Eight patients with secondary AIN met the criteria for Inborn Errors of Immunity. Conclusion: AIN prevalence was 2 %. Most cases were first evaluated by a pediatric immunologist or rheumatologist rather than a pediatric hematologist. This study highlights the need for a multidisciplinary approach involving a pediatric immunologist, rheumatologist, and hematologist. Objective (CN 1 years hematology rheumatology 2018 3 2019 Methods patient s charts (ANC 15 1. 109L L 109 (AIN stimulation disorders Fishers Fisher variables Results 1039 039 1,03 20 Twentyone Twenty one ( 386 38 6 38. followup, followup follow up, follow-up 42 4 4. p 003. 003 . 003) (3 196 19 19. Immunity Conclusion hematologist 201 10 103 03 1,0 00 0

Finos de carvão vegetal são coprodutos da carbonização da madeira, indesejados por diminuir o rendimento gravimétrico em carvão vegetal. São produzidos devido ao processo de carbonização, durante o armazenamento e transporte do carvão vegetal. A pelletização de finos aumenta a densidade energética, produzindo um combustível homogêneo e com menor teor de umidade, possibilitando a queima em equipamentos com alta eficiência energética, além de propriedades desejáveis em produtos energéticos como alto teor de carbono fixo e baixo teor de umidade. Este estudo avaliou o efeito do teor de umidade e do tempo de secagem na produção de black pellets a partir de finos de carvão vegetal ligados com amido de arroz (Oryza sativa) nas características físico-químicas e mecânicas. Os black pellets foram produzidos utilizando duas proporções de água (10 ou 20%) e tempo de secagem de 4 ou 6 horas. Os black pellets foram avaliados quanto à análise química imediata, composição elementar, teor de umidade, tamanho, densidade aparente, poder calorífico e densidade energética. A adição de água não afetou o teor de voláteis, cinzas e carbono fixo, enquanto o tratamento com adição de 20% de água e quatro horas de secagem apresentou maior teor de umidade (3,34%). A adição de água permitiu melhor condução de calor e disposição das partículas, produzindo pellets com durabilidade superior a 98%. A melhor disposição promovida pela adição de 20% de água também aumentou a densidade dos pellets, resultando em maior densidade energética. Os black pellets podem ser considerados uma boa alternativa para aquecimento residencial, com tratamento T3 (20% de umidade - 4h de secagem) apresentando elevada densidade energética e melhor desempenho em termos de propriedades desejáveis para utilização e comercialização de acordo com a norma EN 14961-6.

Charcoal fines are co-products of the wood carbonization process, unwanted because they reduce the gravimetric yield of the main product, charcoal. It can be produced due to the carbonization process, during the storage and transport of charcoal. Pelletizing charcoal fines increases the energy density of this material, producing a homogeneous fuel with lower moisture content, enabling burning in equipment with high energy efficiency, in addition to desirable properties in energy products such as high fixed carbon content and low moisture content. This study evaluated the effect of moisture content and drying time on the production of black pellets from charcoal fines bound with rice starch (Oryza sativa) on the physicochemical and mechanical characteristics. Black pellets were produced using two proportions of water (10 or 20%) and a drying time of 4 or 6 hours. Black pellets were evaluated for their proximate analysis, elemental composition, moisture content, size, apparent density, calorific value and energy density. The addition of water did not affect the content of volatile matter, ash, and fixed carbon, while the treatment with addition of 20% water and four hours of drying showed a higher moisture content (3.34%). The addition of water allowed for better heat conduction and particle arrangement, producing pellets with a durability greater than 98%. The better arrangement promoted by the addition of 20% water also increased the density of the pellets, resulting in greater energy density. Black pellets can be considered an alternative for residential heating, with T3 treatment (20% - 4h) with a high energy density and better performance in terms of desirable properties for use and commercialization according to the standard EN 14961-6.

ABSTRACT The symbiosis of common beans with nitrogen-fixing bacteria provides an efficient approach to sustainable and economical food production. Therefore, this study aimed to evaluate the application of cost-effective nitrogen management strategies in organic common bean cultivation, including the application of poultry manure and organic liquid fertilizer, seed and co-inoculation with diazotrophic bacteria (Azospirillum brasilense and/or Rhizobium tropici), and supplementary Rhizobium tropici inoculation. The study spanned three years in a well-established organic cultivation field (2018) and an initial organic cultivation area (2019 and 2020) in Brazil. It was arranged in a randomized blocks design in a 2 × 5 (2018) and 2 × 6 (2019 and 2020) factorial scheme, with four replicates. The most profitable strategy involves seed inoculation with Azospirillum brasilense and additional inoculation with Rhizobium tropici at stage V4-5 (fifth trifoliate leaf fully expanded), while the most costly strategy was the application of poultry manure at the V3 stage (first trifoliate leaf fully expanded). Thus, the use of diazotrophic bacteria for seed inoculation and co-inoculation (Azospirillum brasilense and/or Rhizobium tropici) ensured financial returns and system profitability in common bean cultivation. nitrogenfixing fixing production Therefore costeffective cost effective fertilizer coinoculation co andor or tropici, , wellestablished well established 2018 (2018 2019 (201 2020 Brazil scheme replicates V45 V V4 V4- fifth expanded, expanded expanded) first expanded. . Thus 201 (20 202 20 (2 (

RESUMO A simbiose do feijão comum com bactérias fixadoras de nitrogênio proporciona uma abordagem eficiente para a produção de alimentos sustentável e econômica. Portanto, este estudo teve como objetivo avaliar a aplicação de estratégias custo-efetivas de manejo de nitrogênio no cultivo orgânico do feijão comum, incluindo a aplicação de esterco de aves e fertilizante orgânico líquido, inoculação de sementes e coinoculação com bactérias diazotróficas (Azospirillum brasilense e/ou Rhizobium tropici), e inoculação suplementar com Rhizobium tropici. O estudo durou três anos em um campo de cultivo orgânico bem estabelecido (2018) e uma área inicial de cultivo orgânico (2019 e 2020) no Brasil. O delineamento foi em blocos casualizados em esquema fatorial 2 × 5 (2018) e 2 × 6 (2019 e 2020), com quatro repetições. A estratégia mais lucrativa envolve a inoculação de sementes com Azospirillum brasilense e inoculação adicional com Rhizobium tropici no estádio V4-5 (quinta folha trifoliada totalmente expandida), enquanto a estratégia mais cara foi a aplicação de esterco de aves no estádio V3 (primeira folha trifoliada totalmente expandida). Assim, o uso de bactérias diazotróficas para inoculação e coinoculação de sementes (Azospirillum brasilense e/ou Rhizobium tropici) garantiu retorno financeiro e rentabilidade do sistema no cultivo do feijão comum. econômica Portanto custoefetivas custo efetivas líquido eou ou tropici, , 2018 (2018 2019 (201 2020 Brasil 2020, repetições V45 V V4 V4- quinta expandida, expandida expandida) primeira expandida. . Assim 201 (20 202 20 (2 (

Abstract Several topics related to the oral cavity are briefly addressed in this article, from anatomical variations that, when recognized, avoid unnecessary investigations, to diseases that affect exclusively the mouth, mucocutaneous diseases, as well as oral manifestations of systemic diseases. A complete clinical examination comprises the examination of the mouth, and this approach facilitates clinical practice, shortening the path to diagnosis in the outpatient clinic as well as with in-hospital patients. The objective of this article is to encourage the examination of the oral cavity as a useful tool in medical practice, helping to recognize diseases in this location. recognized investigations mouth practice inhospital hospital patients location

ABSTRACT: This study evaluated the probability of pregnancy and associated factors for two times artificial inseminations (AI), 8 or 10 hours after automated activity monitoring (AAM) alarm on the first postpartum AI of 1,054 Holstein dairy cows. The estrus was synchronized by prostaglandin or estradiol-progesterone program. Stepwise logistic regression was performed to analyze the probability of pregnancy, and associated factors (activity, estrus intensity, parity, peripartum health, retained placenta, postpartum vaginal discharge, and season). The highest pregnancy rates were obtained with multiparous animals, inseminated ten hours after the AAM alarm, in the fall or winter season, with a high activity peak and estrus intensity (P < 0.05). Peripartum diseases, retained placenta, and postpartum vaginal discharge negatively influenced the pregnancy rate, regardless of parity. Thus, the optimization of AAM models by including on-farm measures like parity, peripartum health history, and environmental conditions may favor the correct identification of estrus and improve the AAM alarm regarding the ideal moment for AI, increasing the reproductive performance in dairy cows. ABSTRACT , (AI) 1 (AAM 1054 054 1,05 cows estradiolprogesterone estradiol progesterone program activity, (activity parity placenta season. season . season) animals P 0.05. 005 0.05 0 05 0.05) diseases rate Thus onfarm farm history (AI 105 1,0 00 0.0 1, 0.

RESUMO: O objetivo deste estudo foi avaliar a probabilidade de prenhez e fatores associados para dois horários de inseminação artificial (IA), oito ou 10 horas após o alarme de monitoramento automatizado de atividades (AAM), na primeira IA pós-parto em 1.054 vacas leiteiras da raça Holandês. O estro foi sincronizado por protocolos a base de prostaglandina ou estradiol-progesterona. Regressão logística stepwise foi realizada para analisar a probabilidade de prenhez e fatores associados (atividade, intensidade do estro, paridade, saúde no periparto, placenta retida, descarga vaginal pós-parto e estação do ano). As maiores taxas de prenhez foram obtidas em multíparas, inseminadas 10 horas após o alarme do AAM, no outono ou inverno, com pico de atividade elevado e intensidade de estro (P < 0,05). Doenças no periparto, placenta retida e descarga vaginal pós-parto influenciaram negativamente a taxa de prenhez, independentemente da paridade. Assim, a otimização dos modelos de AAM, incluindo medidas rotineiras da fazenda como paridade, histórico de saúde no periparto e condições ambientais, podem favorecer a identificação correta do estro e melhorar o alarme do AAM em relação ao momento ideal para a IA, aumentando o desempenho reprodutivo nas vacas leiteiras. RESUMO , (IA) 1 (AAM) pósparto pós parto 1054 054 1.05 Holandês estradiolprogesterona. estradiolprogesterona estradiol progesterona. progesterona estradiol-progesterona atividade, (atividade paridade ano. ano . ano) multíparas inverno P 0,05. 005 0,05 0 05 0,05) Assim ambientais (IA (AAM 105 1.0 00 0,0 1. 0,

ABSTRACT We report a case of eosinophilic meningitis associated with the ingestion of raw fish (Cichla sp.) from the Brazilian Amazon, likely caused by Gnathostoma. A 36-year-old male visited Juruena river on a fishing trip. After 50 days, the patient presented with an intense frontal headache. A cerebrospinal fluid examination revealed 63% eosinophilia. Another individual who ingested raw fish developed linear dermatitis on the abdominal wall. Anti-Gnathostoma serum antibodies were detected, and the patient made a full recovery after treatment with corticosteroids and albendazole. To date, autochthonous Gnathostoma spp. infections in Latin American countries have only caused linear panniculitis. This report raises awareness of gnathostomiasis-causing meningitis. Cichla sp. sp Amazon 36yearold yearold 36 year old trip 5 days headache 63 eosinophilia wall AntiGnathostoma Anti detected albendazole date spp panniculitis gnathostomiasiscausing gnathostomiasis causing 3 6

ABSTRACT Purpose The objective of this study was analyzed the neuropsychological functions of students from a public school in Brazil, enrolled in the 1st and 2nd year of Elementary School at the time of the reopening of schools during the COVID-19 pandemic and to access the influence of family and contextual information on the performance of these skills. Methods 117 students participated in the study, as well as their parents or guardians. The children were evaluated in person using the Brief Child Neuropsychological Assessment Instrument (NEUPSILIN-Inf). The parents/guardians answered remotely the Inventory of Resources of the Family Environment and questions about socioeconomic classification and maternal education. Results The data showed a high prevalence of children who had problems or deficits in the functions of orientation, memory, language, visuospatial skills, arithmetic skills and verbal fluency. Furthermore, predictable activities that signal some degree of stability in family life are predictors of children's performance in orientation skills and resources that promote proximal processes significantly reflect on language performance. The results suggest that children included in families with a household income below one Brazilian minimum monthly salary presented poorer inhibitory control performances. Conclusion The impact of changes in neuropsychological skills in children's learning were presented and discussed, highlighting the need for immediate and targeted intervention of these functions. Contextual factors that influenced the performance of neuropsychological skills were also considered. Brazil st nd COVID19 COVID 19 COVID-1 11 guardians NEUPSILINInf. NEUPSILINInf NEUPSILIN Inf . (NEUPSILIN-Inf) parentsguardians education memory fluency Furthermore childrens s performances discussed considered COVID1 1 COVID- (NEUPSILIN-Inf

RESUMO Objetivo O objetivo desse estudo foi analisar as funções neuropsicológicas de estudantes de uma escola pública do Distrito Federal (Brasil), matriculadas 1º e 2º ano de Ensino Fundamental na reabertura das escolas públicas na pandemia da COVID-19 e a influência dos fatores familiares e contextuais sobre o desempenho dessas habilidades. Métodos Participaram 117 estudantes, bem como seus responsáveis. As crianças foram avaliadas presencialmente por meio do Instrumento de Avaliação Neuropsicológica Breve Infantil (NEUPSILIN-Inf). Os pais/responsáveis responderam remotamente ao Inventário de Recursos do Ambiente Familiar e as questões para classificação socioeconômica e de escolaridade materna. Resultado Os dados apontam alta prevalência de crianças em fase de alfabetização que apresentam alerta ou déficit das funções de orientação, memória, linguagem, habilidades visuoespaciais, habilidades aritméticas e fluência verbal. Ainda, as atividades previsíveis que sinalizam algum grau de estabilidade na vida familiar são preditoras do desempenho das crianças na habilidade de orientação e os recursos que promovem processos proximais refletem significativamente no desempenho em linguagem. Os resultados sugerem que crianças inseridas em família com estimativas da renda domiciliar abaixo de um salário mínimo apresentam piores desempenhos do controle inibitório. Conclusão Foram apresentados e discutidos os impactos das alterações das habilidades neuropsicológicas para o aprendizado das crianças, destacando a necessidade de intervenções imediatas e direcionadas para essas funções. Foram considerados os fatores contextuais que apresentaram influência sobre o desempenho das habilidades neuropsicológicas. Brasil, Brasil , (Brasil) º COVID19 COVID 19 COVID-1 11 responsáveis NEUPSILINInf. NEUPSILINInf NEUPSILIN Inf . (NEUPSILIN-Inf) paisresponsáveis pais materna memória linguagem visuoespaciais verbal Ainda inibitório (Brasil COVID1 1 COVID- (NEUPSILIN-Inf

Resumen La temperatura del aire es un factor climático que afecta la incidencia del dengue, con efectos que varían según el tiempo y el territorio. Investigamos la relación entre la temperatura mínima del aire y la incidencia de la enfermedad en Minas Gerais, Brasil, y evaluamos la influencia de variables socioeconómicas y geográficas en esta relación. Se trata de un estudio de serie temporal cuyo análisis se realiza en tres etapas distintas: modelación mediante el uso de distributed lag non-linear model (modelos distributivos no lineales con retraso), metaanálisis de los modelos obtenidos y metarregresión con datos geográficos y socioeconómicos. La temperatura mínima fue un factor de protección ante temperaturas extremadamente frías (RR = 0,65; IC95%: 0,56-0,76) y moderadas (RR = 0,71; IC95%: 0,64-0,79) y factor de riesgo en temperaturas de calor moderado (RR = 1,15; IC95%: 1,07-1,24), pero no en extremo (RR = 1,1; IC95%: 0,99-1,22). La heterogeneidad de los modelos fue alta (I2 = 60%), y esta medida no se modificó en la metarregresión. Las temperaturas frías moderadas y extremas tienen un efecto protector, mientras que las temperaturas moderadamente altas aumentan el riesgo. Sin embargo, la temperatura mínima del aire no explica la variabilidad de la región, ni siquiera con las demás variables en metarregresión. dengue territorio Gerais Brasil distintas nonlinear non linear retraso, retraso , retraso) socioeconómicos RR 0,65 065 0 65 IC95% IC95 IC 0,560,76 056076 0,56 0,76 56 76 0,56-0,76 0,71 071 71 0,640,79 064079 0,64 0,79 64 79 0,64-0,79 1,15 115 1 15 1,071,24, 107124 1,07 1,24 07 24 1,07-1,24) 1,1 11 0,991,22. 099122 0,99 1,22 . 99 22 0,99-1,22) I2 I (I 60%, 60 60% 60%) protector embargo región 0,6 06 6 IC9 560 0,560,7 05607 056 0,5 076 0,7 5 7 0,56-0,7 640 0,640,7 06407 064 079 0,64-0,7 1,071,24 10712 107 1,0 124 1,2 2 1,07-1,24 1, 991 0,991,22 09912 099 0,9 122 9 0,99-1,22 0, 0,560, 0560 05 0,56-0, 0,640, 0640 0,64-0, 1,071,2 1071 10 12 1,07-1,2 0,991,2 0991 09 0,99-1,2 0,560 0,56-0 0,640 0,64-0 1,071, 1,07-1, 0,991, 0,99-1, 0,56- 0,64- 1,071 1,07-1 0,991 0,99-1 1,07- 0,99-

Abstract Air temperature is a climatic factor that affects the incidence of dengue, with effects varying according to time and space. We investigated the relationship between minimum air temperature and dengue incidence in Minas Gerais, Brazil, and evaluated the influence of socioeconomic and geographic variables on this relationship. This is a time series study with analysis conducted in three distinct stages: modeling using a distributed lag non-linear model, meta-analysis of models obtained, and meta-regression with geographic and socioeconomic data. Minimum temperature was a protective factor at extreme cold temperatures (RR = 0.65; 95%CI: 0.56-0.76) and moderate cold temperatures (RR = 0.71; 95%CI: 0.64-0.79), and a risk factor at moderate hot temperatures (RR = 1.15; 95%CI: 1.07-1.24), but not at extreme hot temperatures (RR = 1.1; 95%CI: 0.99-1.22). Heterogeneity of the models was high (I2 = 60%), which was also observed in meta-regression. Moderate and extreme cold temperatures have a protective effect, while moderate hot temperatures increase the risk. However, minimum air temperature does not explain the variability in the region, not even with the other variables in meta-regression. space Gerais Brazil stages nonlinear non linear model metaanalysis meta obtained metaregression regression data RR 0.65 065 0 65 95%CI 95CI CI 95 0.560.76 056076 0.56 0.76 56 76 0.56-0.76 0.71 071 71 0.640.79, 064079 0.64 0.79 , 64 79 0.64-0.79) 1.15 115 1 15 1.071.24, 107124 1.07 1.24 07 24 1.07-1.24) 1.1 11 0.991.22. 099122 0.99 1.22 . 99 22 0.99-1.22) I2 I (I 60%, 60 60% 60%) metaregression. regression. effect However region 0.6 06 6 9 560 0.560.7 05607 056 0.5 076 0.7 5 7 0.56-0.7 640 0.640.79 06407 064 079 0.64-0.79 1.071.24 10712 107 1.0 124 1.2 2 1.07-1.24 1. 991 0.991.22 09912 099 0.9 122 0.99-1.22 0. 0.560. 0560 05 0.56-0. 0.640.7 0640 0.64-0.7 1.071.2 1071 10 12 1.07-1.2 0.991.2 0991 09 0.99-1.2 0.560 0.56-0 0.640. 0.64-0. 1.071. 1.07-1. 0.991. 0.99-1. 0.56- 0.640 0.64-0 1.071 1.07-1 0.991 0.99-1 0.64- 1.07- 0.99-

Resumo A temperatura do ar é um fator climático que afeta a incidência da dengue, com efeitos variando conforme o tempo e o espaço. Investigamos a relação entre a temperatura mínima do ar e a incidência da doença em Minas Gerais, Brasil, e avaliamos a influência de variáveis socioeconômicas e geográficas nessa relação, calculando-se o risco relativo (RR). Este é um estudo de série temporal com análise conduzida em três etapas distintas: modelagem por uso de distributed lag non-linear model (modelos não-lineares distributivos com defasagem), metanálise dos modelos obtidos e metarregressão com dados geográficos e socioeconômicos. A temperatura mínima foi um fator de proteção quando em temperaturas frias extremas (RR = 0,65; IC95%: 0,56-0,76) e moderadas (RR = 0,71; IC95%: 0,64-0,79) e fator de risco em temperaturas de calor moderado (RR = 1,15; IC95%: 1,07-1,24), mas não em extremo (RR = 1,1; IC95%: 0,99-1,22). A heterogeneidade dos modelos foi elevada (I2 = 60%) e essa medida não foi alterada em metarregressão. Temperaturas frias moderadas e extremas causam efeito protetivo, enquanto moderadas quentes aumentam o risco. No entanto, a temperatura mínima do ar não explica nem a variabilidade da região, nem mesmo com as outras variáveis em metarregressão. dengue espaço Gerais Brasil calculandose calculando se RR. RR . (RR) distintas nonlinear non linear nãolineares lineares defasagem, defasagem , defasagem) socioeconômicos 0,65 065 0 65 IC95% IC95 IC 0,560,76 056076 0,56 0,76 56 76 0,56-0,76 0,71 071 71 0,640,79 064079 0,64 0,79 64 79 0,64-0,79 1,15 115 1 15 1,071,24, 107124 1,07 1,24 07 24 1,07-1,24) 1,1 11 0,991,22. 099122 0,99 1,22 99 22 0,99-1,22) I2 I (I 60% 60 protetivo entanto região 0,6 06 6 IC9 560 0,560,7 05607 056 0,5 076 0,7 5 7 0,56-0,7 640 0,640,7 06407 064 079 0,64-0,7 1,071,24 10712 107 1,0 124 1,2 2 1,07-1,24 1, 991 0,991,22 09912 099 0,9 122 9 0,99-1,22 0, 0,560, 0560 05 0,56-0, 0,640, 0640 0,64-0, 1,071,2 1071 10 12 1,07-1,2 0,991,2 0991 09 0,99-1,2 0,560 0,56-0 0,640 0,64-0 1,071, 1,07-1, 0,991, 0,99-1, 0,56- 0,64- 1,071 1,07-1 0,991 0,99-1 1,07- 0,99-

Resumen: Este estudio tuvo como objetivo investigar las asociaciones entre las percepcion del vecindario y los problemas de sueño en ancianos brasileños. Se realizó un estudio transversal con 5.719 ancianos de la comunidad (≥ 60 años) del Estudio Longitudinal de Salud de los Ancianos Brasileños (ELSI-Brasil, 2019-2021). Los desenlaces fueron los siguientes: problemas de sueño autoinformados: mala calidad del sueño, somnolencia diurna, quejas de insomnio primario, dificultad para permanecer dormido y despertarse durante la madrugada. Las variables de exposición fueron las siguientes: preguntas sobre la percepción de los participantes sobre el entorno físico y social del vecindario. En el análisis de datos se utilizó la regresión logística. La basura, la suciedad o el césped alto en las calles y el deseo de cambiar el lugar donde viven se asociaron con mayores probabilidades de tener una mala calidad del sueño. La preocupación por las caídas debido a aceras en mal estado, la preocupación por las dificultades para utilizar los medios de transporte y la preocupación por las dificultades para cruzar la calle se asociaron con mayores probabilidades de sufrir todos los problemas de sueño. El ruido producido por los autobuses y automóviles se asoció con una mayor probabilidad de sufrir algunos problemas de sueño. La percepción del vecindario como un buen lugar para vivir se asoció con menores probabilidades de sufrir somnolencia diurna y quejas de insomnio primario. La confianza en la mayoría de la gente del vecindario y la percepción de que los niños y jóvenes tratan a los adultos con respeto se asociaron con menores probabilidades de sufrir somnolencia diurna, quejas de insomnio primario y despertarse durante la madrugada. Un buen lugar para que los niños jugaran y para que los adolescentes crecieran se asoció con menores probabilidades de sufrir somnolencia diurna. Estos resultados pueden ayudar a los gestores públicos a crear políticas de planificación urbana dirigidas a mejorar los entornos vecinales como forma de promover la salud. Resumen brasileños 5719 5 719 5.71 ≥ ( 6 años ELSIBrasil, ELSIBrasil ELSI Brasil, Brasil (ELSI-Brasil 20192021. 20192021 2019 2021 . 2019-2021) siguientes autoinformados madrugada logística basura estado salud 571 71 5.7 2019202 201 202 2019-2021 57 7 5. 201920 20 2019-202 20192 2 2019-20 2019-2 2019-

Abstract: This study aimed to investigate associations between neighborhood perception and sleep problems in older Brazilian adults. A cross-sectional study was conducted with 5,719 community-dwelling older adults (≥ 60 years) from the Brazilian Longitudinal Study of Aging (ELSI-Brazil, 2019-2021). The outcomes were self-reported sleep problems: poor sleep quality, daytime sleepiness, primary insomnia complaints, difficulty staying asleep, and waking up at dawn. The exposure variables were questions about the perception of participants about the physical and social environment of the neighborhood. Logistic regression was used in data analysis. Garbage, rubbish, or tall grass on the streets and the desire to move were associated with higher odds of poor sleep quality. Concern about falling due to damaged sidewalks, concern about having difficulties taking transportation, and concern about having difficulties crossing the street were associated with higher odds of all sleep problems. Sound/noise of buses and cars was associated with higher odds of some sleep problems. Perceiving the neighborhood as a good place to live was associated with lower odds of daytime sleepiness and primary insomnia complaints. Trusting most people in the neighborhood and perceiving that kids and younger people treat adults with respect were associated with lower odds of daytime sleepiness, primary insomnia complaints, and waking up at dawn. Being a good place for kids to play and raise teenagers was associated with lower odds of daytime sleepiness. These results can assist public administrators in creating urban planning policies aimed at improving neighborhood environments as a means of health promotion. Abstract crosssectional cross sectional 5719 5 719 5,71 communitydwelling community dwelling ≥ ( 6 years ELSIBrazil, ELSIBrazil ELSI Brazil, Brazil (ELSI-Brazil 20192021. 20192021 2019 2021 . 2019-2021) selfreported self reported quality complaints asleep dawn analysis Garbage rubbish sidewalks transportation Soundnoise Sound noise promotion 571 71 5,7 2019202 201 202 2019-2021 57 7 5, 201920 20 2019-202 20192 2 2019-20 2019-2 2019-

Resumo: Este estudo teve como objetivo investigar associações entre percepções da vizinhança e problemas de sono em idosos brasileiros. Foi realizado um estudo transversal com 5.719 idosos da comunidade (≥ 60 anos) do Estudo Longitudinal da Saúde dos Idosos Brasileiros (ELSI-Brasil, 2019-2021). Os desfechos foram problemas de sono autorreferidos: má qualidade do sono, sonolência diurna, queixas de insônia primária, dificuldade em manter o sono e acordar na madrugada. As variáveis de exposição foram: questões sobre a percepção dos participantes sobre o ambiente físico e social da vizinhança. Regressão logística foi utilizada na análise dos dados. Lixo, sujeira ou grama alta nas ruas e o desejo de mudar de onde mora foram associados a maiores chances de má qualidade do sono. Preocupação em cair devido a calçadas em má condição, preocupação com dificuldades para usar os meios de transporte e preocupação com dificuldades para atravessar a rua foram associadas a maiores chances de todos os problemas de sono. O som/ruído dos ônibus e carros foi associado a maiores chances de alguns problemas de sono. A percepção da vizinhança como um bom lugar para morar foi associado a menores chances de sonolência diurna e queixas de insônia primária. A confiança na maioria das pessoas da vizinhança e a percepção de que crianças e jovens tratam os adultos com respeito foram associados a menores chances de sonolência diurna, queixas de insônia primária e acordar na madrugada. Um bom lugar para as crianças brincarem e para os adolescentes crescerem foi associado a menores chances de sonolência diurna. Esses resultados podem ajudar os gestores públicos na criação de políticas de planejamento urbano voltadas a melhorias nos ambientes da vizinhança como forma de promoção da saúde. Resumo brasileiros 5719 5 719 5.71 ≥ ( 6 anos ELSIBrasil, ELSIBrasil ELSI Brasil, Brasil (ELSI-Brasil 20192021. 20192021 2019 2021 . 2019-2021) autorreferidos madrugada dados Lixo condição somruído som ruído saúde 571 71 5.7 2019202 201 202 2019-2021 57 7 5. 201920 20 2019-202 20192 2 2019-20 2019-2 2019-

Abstract The adnexa fetal tissues are sources of mesenchymal stromal cells (MSCs) due to their noninvasive harvest, with all biological material discarded most of the time. MSCs are a promise regarding to their plasticity, self-renewal, differentiation potentials, immunomodulatory and anti-inflammatory properties, which have made clinical stem cell therapy a reality. The present study aimed to characterize and evaluate the immunomodulation ability of bovine mesenchymal cells collected from bovine amniotic fluid (bAFMSCs) isolated and subjected to sixth consecutive culture passages in vitro. The multilineage properties of the bAFMSCs collections confirmed the ability to undergo adipogenic, chondrogenic and osteogenic differentiation. The mesenchymal gene transcription CD106, CD73, CD29, CD90 and CD166 were detected in bAFMSCs, whereas CD34 and CD45 were not detected. Regarding cytokine mRNA expression, IL2, IL6, INFα, INFβ, INFγ, TNFα and TNFβ were downregulated, while IL10 was highly regulated in all studied passages. The present study demonstrated the immunological properties and multipotency of in vitro bAFMSCs collections, and thus, they can be tested in cattle pathological treatments or multiplication by nuclear transfer cloning. (MSCs harvest time plasticity selfrenewal, selfrenewal self renewal, renewal self-renewal potentials antiinflammatory anti inflammatory reality (bAFMSCs adipogenic CD106 CD CD73 CD29 CD9 CD16 CD3 CD4 expression IL2 IL IL6 INFα INFβ INFγ downregulated IL1 thus cloning CD10 CD7 CD2 CD1

RESUMEN Objetivo: desarrollar y validar un juego educativo capaz de apoyar acciones de educación en salud para pacientes sometidos a trasplante de hígado. Método: estudio de un producto tecnológico, basado en el marco del Diseño Centrado en el Usuario, realizado en un hospital de referencia para trasplante hepático. Los participantes fueron pacientes sometidos a trasplante de hígado, enfermeras y profesionales de tecnologías de la información. La recolección de datos se realizó a través de entrevistas semiestructuradas y cuestionario, utilizando estándares brasileños para la evaluación de la usabilidad. El análisis de los datos ocurrió a través del análisis de contenido, basado en el modelo de cálculo de la System Usability Scale. Resultados: participaron 17 pacientes, entre ellos siete enfermeras y cuatro profesionales de tecnologías de la información. El juego se compone de cinco recorridos cuyo cuidado principal se realizará en casa. Cada camino presenta pasos a seguir con sus reglas. En cuanto a la validación, la media general fue de 1,38. La valoración de la usabilidad por parte de los pacientes tuvo una media de 80 puntos, mientras que la de los profesionales superó el 75%. Conclusión: se considera que la herramienta antes mencionada es susceptible de ser utilizada en la práctica por los usuarios. Además, esto se ajustó para satisfacer las necesidades de los pacientes sometidos a trasplante de hígado para apoyar y gestionar la atención domiciliaria. Objetivo Método tecnológico Usuario hepático información cuestionario contenido Scale Resultados 1 casa reglas validación 138 38 1,38 8 puntos 75 75% Conclusión usuarios Además domiciliaria 13 3 1,3 7 1,

ABSTRACT Objective: to develop and validate an educational game capable of supporting health education actions for patients undergoing liver transplantation. Method: this is a study of a technological product, based on the User-Centered Design framework, carried out in a reference hospital for liver transplantation. Participants were patients undergoing liver transplantation, nurses and information technology professionals. Data collection took place through semi-structured interviews and a questionnaire, using Brazilian standards for usability assessment. Data analysis occurred through content analysis, based on the calculation model for the System Usability Scale. Results: seventeen patients participated, including seven nurses and four information technology professionals. The game is made up of five paths with the main care to be carried out at home. Each path presents steps to be followed with its rules. As for validation, the overall mean was 1.38. Usability assessment by patients had a mean of 80 points, while by professionals it was above 75%. Conclusion: it is considered that the aforementioned tool is capable of being used in practice by users. Furthermore, this was adjusted to meet the needs of patients undergoing liver transplantation to support and manage home care. Objective Method product UserCentered User Centered framework semistructured semi structured questionnaire Scale Results participated rules validation 138 1 38 1.38 8 points 75 75% Conclusion users Furthermore 13 3 1.3 7 1.

RESUMO Objetivo: desenvolver e validar jogo educativo capaz de subsidiar ações de educação em saúde ao paciente submetido ao transplante hepático. Método: estudo de produto tecnológico, sustentado no referencial doDesign Centrado no Usuário, realizado em hospital de referência em transplante hepático. Participantes foram pacientes submetidos ao transplante hepático, enfermeiros e profissionais da tecnologia da informação. A coleta de dados ocorreu por meio de entrevista semiestruturada e questionário, utilizando as normas brasileiras para avaliação da usabilidade. A análise dos dados ocorreu por meio de análise de conteúdo, com base no modelo de cálculo para a escala de System Usability Scale. Resultados: participaram 17 pacientes, dos quais, sete enfermeiros e quatro profissionais da tecnologia da informação. O jogo é formado por cinco caminhos com os principais cuidados a serem desenvolvidos em domicílio. Cada caminho apresenta etapas a serem seguidas com suas regras. Quanto à validação, a média geral foi de 1,38. A avaliação da usabilidade pelos pacientes teve média de 80 pontos, já pelos profissionais foi acima de 75%. Conclusão: considera-se que a referida ferramenta está apta para ser usada na prática pelos usuários. Ainda, essa foi ajustada para atender às necessidades dos pacientes submetidos ao transplante hepático para apoiar e gerenciar os cuidados em domicílio. Objetivo Método tecnológico Usuário informação questionário conteúdo Scale Resultados 1 quais domicílio regras validação 138 38 1,38 8 pontos 75 75% Conclusão considerase considera se usuários Ainda 13 3 1,3 7 1,

ABSTRACT The standardized precipitation (SPI) and standardized precipitation-evapotranspiration (SPEI) indices are important tools for monitoring drought events, but the low density of weather station networks limits their use in many regions. To address this issue, we developed the PowerSDI R-package, which calculates these two indices using gridded-data from the NASA-POWER project (NASA-SPI and NASA-SPEI). Different from other packages, the PowerSDI package has two modes: the scientific, and the operational. In the scientific mode, the users may assess the quality of the indices estimates through their agreement with a reference/observed series and through the evaluation of how well these estimates meet the conceptual assumptions required for calculating both SPI and SPEI. This evaluation is based on measure of accuracy (e.g., Willmott index of agreement), goodness-of-fit tests (e.g., Anderson-Darling), and normality tests (e.g., Shapiro-Wilk’s test), which are calculated by the ScientSDI.R, Reference.R, and Accuracy.R functions. In the operational mode, users can calculate both indices routinely using the OperatSDI.R function. The package also uses a quasi-weekly time scale, allowing for index calculations four times a month. The OperatSDI.R enables users to download NASA-POWER data for all available period or only for the quasi-week they intend to monitor (reducing the function’s running time). In short, the PowerSDI facilitates the routine use of these two widely used drought indices and, unlike others existing software, it provides a solid scientific basis for using NASA-POWER data in drought monitoring systems, which can help improve drought preparedness and response efforts worldwide. The package is freely available at two repositories: Github (https://github.com/gabrielblain/PowerSDI), and CRAN (https://CRAN.R-project.org/package=PowerSDI). (SPI precipitationevapotranspiration evapotranspiration SPEI (SPEI events regions issue Rpackage, Rpackage R package, R-package griddeddata gridded NASAPOWER NASA POWER NASASPI NASASPEI. NASASPEI . NASA-SPEI) packages modes mode referenceobserved reference observed e.g., eg e g (e.g. agreement, , agreement) goodnessoffit goodness fit AndersonDarling, AndersonDarling Anderson Darling Anderson-Darling) ShapiroWilks Shapiro Wilk s test, test test) ScientSDIR ScientSDI ScientSDI.R ReferenceR Reference Reference.R AccuracyR Accuracy functions OperatSDIR OperatSDI function quasiweekly quasi weekly scale month quasiweek week reducing time. time) short software systems worldwide repositories https//github.com/gabrielblain/PowerSDI, httpsgithubcomgabrielblainPowerSDI https //github.com/gabrielblain/PowerSDI github com gabrielblain (https://github.com/gabrielblain/PowerSDI) https//CRAN.Rproject.org/package=PowerSDI. httpsCRANRprojectorgpackagePowerSDI //CRAN.R project.org/package=PowerSDI org (https://CRAN.R-project.org/package=PowerSDI) NASA-SPEI e.g. (e.g Anderson-Darling https//github.com/gabrielblain/PowerSDI githubcomgabrielblainPowerSDI (https://github.com/gabrielblain/PowerSDI Rproject https//CRAN.Rproject.org/package=PowerSDI CRANR projectorgpackagePowerSDI (https://CRAN.R-project.org/package=PowerSDI e.g