RESUMO Objetivo: Verificar a utilização e identificar as vantagens dos métodos moleculares para diagnóstico de infecções congênitas no líquido cefalorraquidiano de neonatos. Fontes de dados: A revisão foi registrada na base PROSPERO (International Prospective Register of Systematic Reviews) sob CRD42021274210. A busca bibliográfica foi realizada nas bases de dados PubMed, Biblioteca Virtual em Saúde/ Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde (BVS/BIREME), Scopus, Web of Science, Excerpta Medica database (EMBASE), Cochrane, ProQuest, e EBSCOhost. A busca foi feita no período de agosto a outubro de 2021 e atualizada em dezembro de 2022, respeitando as orientações do Preferred Reporting Items for Systematic Reviews e Meta-Analyises (PRISMA). A sequência da seleção dos estudos foi: 1) Remoção de duplicatas; 2) Exame de títulos e resumos; 3) Recuperação dos textos completos potencialmente relevantes; e 4) Avaliação do texto completo conforme critérios de elegibilidade por dois autores independentes. O critério de inclusão considerou ensaios clínicos randomizados e não randomizados, estudos longitudinais, transversais, revisados por pares, estudos em humanos, publicados em inglês, espanhol, italiano e português, com recém-nascidos de até 28 dias que sofreram neuroinfecções congênitas pelos agentes toxoplasmose, rubéola, citomegalovírus, herpes simples (TORCH), e outros como Treponema pallidum, Zika, parvovírus B-19, varicela zoster, Epstein-Barr, e SARS-CoV-2, diagnosticadas por reação em cadeia de polimerase (PCR). Dois avaliadores extraíram as seguintes informações: autor, ano de publicação, nacionalidade, sujeitos, tipo de estudo, métodos, resultados e conclusão. Síntese dos dados: O patógeno mais estudado foi Herpes Simples. Muitos artigos relataram somente sintomas iniciais inespecíficos, motivando a coleta de líquido cefalorraquidiano e realização da PCR para investigação etiológica. Conclusões: Os métodos moleculares são eficazes para detectar o genoma do patógeno no líquido cefalorraquidiano, o que pode impactar na evolução clínica e no prognóstico neurológico. Objetivo neonatos International CRD42021274210 CRD PubMed LatinoAmericano Latino Americano BVS/BIREME, BVSBIREME BVS/BIREME , BVS BIREME (BVS/BIREME) Scopus Science EMBASE, EMBASE (EMBASE) Cochrane ProQuest EBSCOhost 202 2022 MetaAnalyises Meta Analyises PRISMA. PRISMA . (PRISMA) 1 duplicatas 2 resumos 3 relevantes 4 independentes longitudinais transversais pares humanos inglês espanhol português recémnascidos recém nascidos toxoplasmose rubéola citomegalovírus TORCH, TORCH (TORCH) pallidum Zika B19, B19 B 19, 19 B-19 zoster EpsteinBarr, EpsteinBarr Epstein Barr, Barr Epstein-Barr SARSCoV2, SARSCoV2 SARSCoV SARS CoV 2, SARS-CoV-2 PCR. (PCR) informações autor publicação nacionalidade sujeitos estudo conclusão Simples inespecíficos etiológica Conclusões neurológico CRD4202127421 (BVS/BIREME (EMBASE 20 (PRISMA (TORCH B1 B-1 SARS-CoV- (PCR CRD420212742 B- SARS-CoV CRD42021274 CRD4202127 CRD420212 CRD42021 CRD4202 CRD420 CRD42 CRD4

ABSTRACT Objective: To verify the use and identify advantages of molecular methods for congenital infections diagnosis in cerebrospinal fluid of neonates. Data source: The review was registered in the International Prospective Register of Systematic Reviews (PROSPERO), under CRD42021274210. The literature search was performed in databases: PubMed, Virtual Health Library/ Latin American and Caribbean Center on Health Sciences Information (VHL/BIREME), Scopus, Web of Science, Excerpta Medica database (EMBASE), Cochrane, ProQuest, and EBSCOhost. The search was carried out from August to October 2021 and updated in December 2022, respecting the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The selection sequence was: 1) Duplicate title removal; 2) Examination of titles and abstracts; 3) Full-text retrieval of potentially relevant reports; and 4) Evaluation of the full text according to eligibility criteria by two independent authors. Inclusion criteria considered randomized and non-randomized control trials, longitudinal, cross-sectional, and peer-reviewed studies in humans, published in English, Spanish, Italian, and Portuguese, with newborns up to 28 days old who had congenital neuroinfections by toxoplasmosis, rubella, cytomegalovirus, herpes simplex (TORCH), and others such as Treponema pallidum, Zika, parvovirus B-19, varicella zoster, Epstein-Barr, and SARS-CoV2, diagnosed by polymerase chain reaction (PCR). Two evaluators extracted the following information: author, year of publication, nationality, subjects, study type, methods, results, and conclusion. Data synthesis: The most studied pathogen was herpes simplex. Several articles reported only nonspecific initial symptoms, motivating the collection of cerebrospinal fluid and performing PCR for etiological investigation. Conclusions: Molecular methods are effective to detect pathogen genomes in cerebrospinal fluid, which can impact clinical evolution and neurological prognosis. Objective neonates source PROSPERO, PROSPERO , (PROSPERO) CRD42021274210 CRD databases PubMed Library VHL/BIREME, VHLBIREME VHL/BIREME VHL BIREME (VHL/BIREME) Scopus Science EMBASE, EMBASE (EMBASE) Cochrane ProQuest EBSCOhost 202 2022 MetaAnalyses Meta Analyses PRISMA (PRISMA guidelines 1 removal 2 abstracts 3 Fulltext Full reports 4 authors nonrandomized non trials longitudinal crosssectional, crosssectional cross sectional, sectional cross-sectional peerreviewed peer reviewed humans English Spanish Italian Portuguese toxoplasmosis rubella cytomegalovirus TORCH, TORCH (TORCH) pallidum Zika B19, B19 B 19, 19 B-19 zoster EpsteinBarr, EpsteinBarr Epstein Barr, Barr Epstein-Barr SARSCoV2, SARSCoV2 SARSCoV SARS CoV2, CoV2 CoV SARS-CoV2 PCR. . (PCR) information author publication nationality subjects type results conclusion synthesis symptoms investigation Conclusions prognosis (PROSPERO CRD4202127421 (VHL/BIREME (EMBASE 20 (TORCH B1 B-1 SARS-CoV (PCR CRD420212742 B- CRD42021274 CRD4202127 CRD420212 CRD42021 CRD4202 CRD420 CRD42 CRD4

In this study, we aimed to investigate the anticancer activity of the extract, fractions, and isolated compounds of the flowers of Fridericia platyphylla, and to characterize the bioactive compounds. The chemical diversity of the extracts and fractions was investigated using liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS). We were able to annotate 26 compounds from the classes of flavones, flavonols, flavanones, isoflavones, and cinnamic acid and its derivatives. The dichloromethane fraction showed greater cytotoxicity at a concentration of 100 µg mL−1. In addition, the inhibitory concentrations of the dichloromethane fraction were 22.14 and 30.9 µg mL−1 for MCF-7 and MDA-MB-231 cell lines, respectively, and were capable of inhibiting tumor cell migration. Brachydins A and C were isolated from the dichloromethane fraction and showed the greatest cytotoxicity. The results obtained from this study show the potential biological effect of F. platyphylla flowers as a possible antitumor pharmacological agent. extract LCHRMS. LCHRMS LC HRMS . (LC-HRMS) 2 flavones flavonols flavanones isoflavones derivatives 10 mL1 mL 1 addition 2214 22 14 22.1 309 30 9 30. mL− MCF7 MCF 7 MCF- MDAMB231 MDAMB MDA MB 231 MDA-MB-23 lines respectively migration F agent (LC-HRMS 221 22. 3 MDAMB23 23 MDA-MB-2 MDAMB2 MDA-MB- MDA-MB

ABSTRACT: To improve the nutritional and functional quality of agro-industrial waste, his study established a ternary mixture of flours made from pineapple, acerola, and passion fruit waste using the simplex-centroid design, as well as to evaluate their effects on the physicochemical and sensory characteristics of edible ice cream. Using mixture planning, nine ternary flour components were applied to edible ice cream formulations to evaluate acceptance scores for taste, texture, and overall impression attributes, as well as physicochemical parameters (pH, acidity, soluble solids, and color parameters) and microbiological parameters (thermotolerant coliforms at 45 ºC, Enterobacteriaceae and Salmonella). The simplex-centroid design demonstrated that the cubic model best predicts the results for the analyzed sensory attributes. The response surface graphs indicated the area of greatest acceptance in the formulations with the maximum percentage established for the flour with acerola waste. The highest level of acceptability was given to the ternary mixture of 60%, 30%, and 10% pineapple, passion fruit, and acerola flours, respectively. The results indicated that sorbet enriched with flour composed of the ternary mixture is a product with the potential for exploitation, as well as contributing to the reduction of environmental impacts generated by the large and improper disposal of agro-industrial fruit processing residues in the ecosystem. ABSTRACT agroindustrial agro industrial pineapple simplexcentroid simplex centroid planning taste texture attributes pH, pH (pH acidity solids thermotolerant 4 ºC Salmonella. Salmonella . Salmonella) 60 60% 30 30% 10 respectively exploitation ecosystem 6 3 1

RESUMO: Visando a qualidade nutricional e funcional dos resíduos agroindustriais, o estudo teve como objetivo estabelecer a mistura ternária de farinhas elaboradas com resíduos de abacaxi, acerola e maracujá utilizando o delineamento simplex-centroide, assim como avaliar seus efeitos nas características físico-químicas e sensoriais em gelados comestíveis. Com o planejamento de misturas, nove componentes ternários das farinhas foram aplicados em formulações de gelados comestíveis para avaliação dos escores de aceitação para os atributos sabor, textura e impressão global, bem como os parâmetros físico-químicos (pH, acidez, sólidos solúveis e parâmetros de cor) e microbiológicos (coliformes termotolerantes a 45 ºC, Enterobacteriaceae e Salmonella). O delineamento simplex-centroide demonstrou que o modelo cúbico melhor prediz os resultados para os atributos sensoriais analisados. Os gráficos de superfície de resposta indicaram a área de maior aceitação nas formulações com o máximo percentual estabelecido para a farinha com os resíduos de acerola. O maior índice de aceitabilidade foi atribuído a mistura ternária constituída com 60%, 30% e 10% para as farinhas de abacaxi, maracujá e acerola, respectivamente. Os resultados indicaram que o sorbet enriquecido com a farinha composta pela mistura ternária é um produto com potencial para exploração, além de contribuir para a redução dos impactos ambientais gerados pelo grande e inadequado descarte de resíduos do processamento agroindustrial de frutas no ecossistema. RESUMO agroindustriais abacaxi simplexcentroide, simplexcentroide simplex centroide, centroide físicoquímicas físico químicas misturas sabor global físicoquímicos químicos pH, pH (pH acidez cor coliformes 4 ºC Salmonella. Salmonella . Salmonella) analisados 60 60% 30 10 respectivamente exploração ecossistema 6 3 1

Resumo O acúmulo de produtos finais de glicação avançada (AGEs, do inglês advanced glycation end-products) provoca comprometimento morfofuncional dos rins. Os níveis de AGEs podem ser estimados de forma não invasiva por autofluorescência da pele (sAF). Exploramos se a sAF elevada prediz desfechos renais em indivíduos com diabetes tipo 2 (DT2). O estudo foi realizado como uma análise predefinida do Estudo Brasileiro sobre Diabetes, uma coorte prospectiva de centro único de adultos com DT2. Foram considerados os dados de 155 indivíduos acompanhados por até 1.716 dias. A incidência de eventos renais adversos maiores (MAKE, por sua sigla em inglês) foi de 9,6%. Indivíduos com sAF acima da mediana apresentaram maior incidência de MAKEs (4,6% vs. 21%; p = 0,002), com HR de 3,39 [IC 95%: 1,06–10,85; p = 0,040] após ajuste por idade e sexo. A alteração média da TFGe ajustada foi de 1,08 unidades (EP: 1,15; IC95%: –1,20, 3,37) no grupo de baixa sAF e de –5,19 unidades [EP: 1,93; IC95%: –9,10, –1,29] no grupo de elevada sAF (diferença entre sujeitos: F: 5,62; p = 0,019). O grupo sAF elevada apresentou maior prevalência de declínio rápido em comparação ao grupo sAF baixa (36,7% vs. 15,8%; p = 0,028). Em conclusão, a sAF elevada foi relacionada ao aumento da incidência de MAKEs e ao declínio mais rápido da TFGe entre indivíduos com DT2. Tal fato deve ser considerado pelos profissionais de saúde ao identificar indivíduos mais propensos a complicações renais relacionadas ao diabetes. AGEs, (AGEs endproducts end products end-products rins sAF. . (sAF) DT2 DT (DT2) Diabetes 15 1716 1 716 1.71 dias MAKE, MAKE (MAKE 96 9 6 9,6% 4,6% 46 4 (4,6 vs 21% 21 0,002, 0002 0,002 , 0 002 0,002) 339 3 39 3,3 IC 95% 95 1,06–10,85 1061085 06 10 85 0,040 0040 040 sexo 108 08 1,0 EP (EP 1,15 115 IC95% IC95 120 20 –1,20 3,37 337 37 519 5 19 –5,1 [EP 1,93 193 93 910 –9,10 –1,29 129 29 diferença sujeitos F 5,62 562 62 0,019. 0019 0,019 019 0,019) 36,7% 367 36 7 (36,7 15,8% 158 8 0,028. 0028 0,028 028 0,028) conclusão (sAF (DT2 171 71 1.7 9,6 4,6 (4, 000 0,00 00 33 3, 1,06–10,8 106108 0,04 004 04 1, 1,1 11 IC9 12 –1,2 51 –5, 1,9 91 –9,1 5,6 56 001 0,01 01 36,7 (36, 15,8 0,02 02 (DT 17 1. 9, 4, (4 0,0 1,06–10, 10610 –1, –5 –9, 5, 36, (36 15, ( 0, 1,06–10 1061 –1 – –9 (3 1,06–1 106 1,06– 1,06

Abstract The accumulation of advanced glycation end-products (AGEs) elicits morphofunctional kidney impairment. AGEs levels can be noninvasively estimated by skin autofluorescence (SAF). We explored whether high SAF predicts kidney outcomes in type 2 diabetes (T2D) individuals. The study was conducted as a predefined analysis of the Brazilian Diabetes Study, a prospective single-center cohort of T2D adults. Data from 155 individuals followed for up to 1716 days were considered. The incidence of major adverse kidney events (MAKE) was 9.6%. Individuals with above-median SAF had a higher incidence of MAKEs (4.6% vs. 21%; p = 0.002), with an HR of 3.39 [95% CI: 1.06–10.85; p = 0.040] after adjustment by age and gender. The mean adjusted eGFR change was 1.08 units (SE: 1.15; 95%CI: –1.20, 3.37) in the low SAF and –5.19 units [SE: 1.93; 95%CI: –9.10, –1.29] in the high SAF groups (between-subject difference: F: 5.62, p = 0.019). The high-SAF group had a greater prevalence of rapid decliners than the low-SAF group (36.7% vs. 15.8%; p = 0.028). In conclusion, high SAF was related to increased incidence of MAKEs and faster decline in eGFR among T2D subjects. This should be considered by healthcare providers when identifying individuals more prone to diabetes-related kidney complications. endproducts end products (AGEs impairment SAF. . (SAF) TD T D (T2D Study singlecenter single center adults 15 171 MAKE (MAKE 96 9 6 9.6% abovemedian above median 4.6% 46 4 (4.6 vs 21% 21 0.002, 0002 0.002 , 0 002 0.002) 339 3 39 3.3 95% 95 [95 CI 1.06–10.85 1061085 1 06 10 85 0.040 0040 040 gender 108 08 1.0 SE (SE 1.15 115 95%CI 95CI 120 20 –1.20 3.37 337 37 519 5 19 –5.1 [SE 1.93 193 93 910 –9.10 –1.29 129 29 betweensubject between subject difference F 562 62 5.62 0.019. 0019 0.019 019 0.019) highSAF lowSAF 36.7% 367 36 7 (36.7 15.8% 158 8 0.028. 0028 0.028 028 0.028) conclusion subjects diabetesrelated complications (SAF 17 9.6 4.6 (4. 000 0.00 00 33 3. [9 1.06–10.8 106108 0.04 004 04 1. 1.1 11 12 –1.2 51 –5. 1.9 91 –9.1 56 5.6 001 0.01 01 36.7 (36. 15.8 0.02 02 9. 4. (4 0.0 [ 1.06–10. 10610 –1. –5 –9. 5. 36. (36 15. ( 0. 1.06–10 1061 –1 – –9 (3 1.06–1 106 1.06– 1.06

ABSTRACT. The objective was to evaluate the effect of probiotic consortium technology (PCT) on the performance of Penaeus vannamei juveniles in the biofloc system in a short time. The experiment had four treatments with four replicates. In each experimental unit, 35 animals (3.15 ± 0.53 g and 7.80 ± 0.54 cm) were stocked in a biofloc system for 35 days. The treatments were: control - control without probiotic; PCT1 - probiotic in the diet (3 mL kg-1) and water (0.5 ppm - three times a week); PCT2 - probiotic in the diet (3 mL kg-1) and water (1 ppm - daily), and PCT3 - probiotic in the water (1 ppm - daily). For water quality and Vibrio, no significant differences were observed among treatments (P > 0.05). Regarding the zootechnical performance, the treatments with the probiotic promoted higher final biomass, biomass gain, and a more efficient feed conversion rate (P < 0.05). The performance improvement promoted by the probiotic may be related to increased concentrations of amylases, chymotrypsin, and lipases in the hepatopancreas (P < 0.05). It was concluded that PCT2 promoted the best zootechnical performance of P. vannamei during the grow-out phase in the biofloc system.

Abstract Multiple pregnancies carry an increased risk of preterm birth, mortality and long-term infant morbidity. We report a case of delayed delivery following a trichorionic triamniotic pregnancy. The pregnant woman was hospitalized due to preterm premature rupture of membranes at 26 weeks. Tocolytics and corticosteroids were provided. Despite these efforts, the first fetus was born vaginally, 48 hours later. After delivery, antibiotics and tocolytics were administered to delay the birth of the remaining fetuses, which occurred after two weeks. All three babies are healthy, with normal neurological development, and the postpartum period was uneventful.

Resumo As gestações múltiplas têm um maior risco de parto prematuro e morbimortalidade neonatal. Este artigo descreve um caso de parto diferido numa gravidez tricoriónica e triamniótica. A grávida foi hospitalizada devido à rutura prematura das membranas às 26 semanas, recebendo tocolíticos e corticosteroides. O primeiro feto nasceu por parto eutócico 48 horas depois. Após o parto, foram administrados antibióticos e tocolíticos, protelando em duas semanas o nascimento dos fetos restantes. Os três bebés estão saudáveis, com desenvolvimento neurológico normal, e o pós-parto decorreu sem complicações.

Abstract To date, hydroxyurea is the only effective and safe drug that significantly reduces morbidity and mortality of individuals with Sickle cell disease. Twenty years of real-life experience has demonstrated that hydroxyurea reduces pain attacks, vaso-occlusive events, including acute chest syndrome, the number and duration of hospitalizations and the need for transfusion. The therapeutic success of hydroxyurea is directly linked to access to the drug, the dose used and adherence to treatment which, in part, is correlated to the availability of hydroxyurea. This consensus aims to reduce the number of mandatory exams needed to access the drug, prioritizing the requesting physician's report, without affecting patient safety. date disease reallife real life attacks vasoocclusive vaso occlusive events syndrome transfusion which part physicians physician s report safety

Resumo Introdução: a associação entre a diminuição da atividade física e o isolamento social já foi descrita na literatura, entretanto a relação do nível de adesão dos idosos às medidas protetivas durante a pandemia da covid-19 e os efeitos ocasionados pela inatividade física na condição de fragilidade permanece com expressivo hiato de conhecimento. Objetivo: analisar os efeitos do distanciamento e do isolamento sociais gerados pela pandemia da covid-19 na fragilidade em idosos e nas atividades físicas realizadas por eles da atenção primária à saúde de uma Unidade Básica de Saúde do Brasil. Materiais e método: estudo de coorte prospectivo, realizado nos domicílios e em unidades básicas de saúde. Na primeira onda, pré-pandemia, a amostra foi composta por idosos (n = 168) não frágeis. Após o seguimento médio de 762 dias, realizou-se uma segunda onda, em que se classificaram os idosos conforme o nível de exposição ao distanciamento e ao isolamento sociais (n = 100), bem como se observaram os desfechos “nível de atividade física” e “condição de fragilidade”. Aplicaram-se questionários sociodemográfico e clínico, miniexame do estado mental, marcadores de fragilidade física e grau de adesão ao distanciamento e ao isolamento sociais. Realizaram se análises estatísticas descritivas, de associação, curvas de sobrevida e regressão de riscos proporcionais. Resultados: 53 % dos idosos transicionaram para a pré-fragilidade e 21 % apresentaram diminuição de atividade física. Houve elevado percentual de baixo grau de adesão ao distanciamento e ao isolamento sociais, e não se observou associação com atividade física e fragilidade (p = 0,288; p = 0,351, respectivamente). Mulheres tiveram mais risco de se classificarem como pré-frágeis ou frágeis (2,53; IC 95 %, 1,01-6,34) e de apresentarem diminuição de atividade física (2,13; IC 95 %, 1,06-4,26). Conclusão: não houve associação entre medidas protetivas de distanciamento e isolamento social, atividade física e fragilidade. Idosos que apresentam redução do nível de atividade física devem ser estimulados à sua prática a fim de se manterem ativos e se evitar a transição para a fragilidade, para a perda de funcionalidade e para a dependência.

Resumen Introducción: la asociación entre la disminución de la actividad física y el aislamiento social ya ha sido descrita en la literatura, sin embargo la relación entre el nivel de adherencia de los ancianos a las medidas de protección durante la pandemia de covid-19 y los efectos causados por la inactividad física sobre la condición de fragilidad permanece con un importante vacío de conocimiento. Objetivo: analizar los efectos del distanciamiento y el aislamiento social generados por la pandemia de covid-19 sobre la fragilidad de los ancianos y las actividades físicas realizadas por ellos en la atención primaria de salud en una Unidad Básica de Salud en Brasil. Materiales y método: estudio de cohorte prospectivo, realizado en domicilios y en unidades de atención primaria de salud. En la primera oleada, prepandémica, la muestra estaba formada por ancianos no frágiles (n = 168). Tras un seguimiento medio de 762 días, se llevo a cabo una segunda etapa, en la que se clasifico a los ancianos en función de su nivel de exposición al distanciamiento social y al aislamiento (n = 100), así como de los resultados “nivel de actividad física” y “estado de fragilidad”. Se utilizaron cuestionarios sociodemográficos y clínicos, miniexamen del estado mental, marcadores de fragilidad física y grado de adhesión al distanciamiento y el aislamiento social. Se realizaron estadísticas descriptivas, análisis de asociación, curvas de supervivencia y regresión de riesgos proporcionales. Resultados: el 53 % de los ancianos pasaron a la situación de prefragilidad y el 21 % mostraron una disminución de la actividad física. Hubo un alto porcentaje de baja adherencia al distanciamiento social y al aislamiento, y no hubo asociación con la actividad física y la fragilidad (p = 0,288; p = 0,351, respectivamente). Las mujeres tenían un mayor riesgo de ser clasificadas como pre-frágiles o frágiles (2,53; IC 95 %, 1,01-6,34) y de mostrar una disminucion de la actividad fisica (2,13; IC 95 %, 1,06-4,26). Conclusión: no hubo asociación entre las medidas protectoras de distanciamiento social y aislamiento, actividad física y fragilidad. Se debería animar a los ancianos con un nivel reducido de actividad física a practicarla para mantenerse activos y evitar la transición a la fragilidad, la perdida de funcionalidad y la dependencia.

Abstract Introduction: The association between reduced physical activity and social isolation has been described in the literature, but the relationship between the level of adherence to protective measures among older adults during the COVID-19 pandemic and the effects of physical inactivity on frailty remains a significant knowledge gap. Objective: To analyze the effects of social distancing and isolation caused by the COVID-19 pandemic on frailty in the elderly and on their physical activities in primary health care in a basic health unit in Brazil. Materials and methods: A prospective cohort study conducted in households and basic health units. In the first wave, before the pandemic, the sample consisted of non-frail elderly (n = 168). After a mean follow-up of 762 days, a second wave was conducted in which the elderly were classified according to their level of exposure to social distancing and isolation (n = 100) and the outcomes “level of physical activity” and “frailty status” were observed. Sociodemographic and clinical questionnaires, the Mini-Mental State Examination, physical markers of frailty, and level of exposure to social distancing and isolation were used. Descriptive statistics, association, survival curves, and proportional hazards regression were used. Results: Of the elderly, 53% transitioned to pre-frailty and 21% showed a decrease in physical activity. There was a high percentage of low adherence to social distancing and isolation, and there was no association with physical activity and frailty (p = 0.288; p = 0.351, respectively). Women had a higher risk of being classified as prefrail or frail (2.53; 95% CI, 1.01-6.34) and of having a decrease in physical activity (2.13; 95% CI, 1.06-4.26). Conclusion: There was no association between protective measures of social distancing and isolation, physical activity, and frailty. Older people with reduced levels of physical activity should be encouraged to exercise to remain active and avoid the transition to frailty, functional decline, and dependency.

ABSTRACT The aim of this study was to evaluate agronomic performance in cultivars of the Cavendish banana under the soil and climate conditions of the microregion of Assis in the state of São Paulo over three production cycles. The experiment was set up at the Fazenda São José Farm, Água do Pau d´Alho, in Palmital, São Paulo. The Grande Naine, IAC2001 and FHIA02 cultivars were evaluated, considering the following variables: cultivar; plant height; pseudostem perimeter; number of leaves at flowering and harvest; number of days from planting to flowering, from planting to harvest, and from flowering to harvest; weight of the bunch, stalk and fruit per bunch; number of hands per bunch; productivity of the fruit and pulp; weight and number of fingers on the second hand; weight, length and diameter of the finger; pulp diameter; and weight and thickness of the peel. The data were subjected to multivariate statistical techniques (principal component factor analysis, tree clustering, two-way joining, and correspondence). The results showed the extraction of three principal factors that accounted for 89.63% of the total variance of the characteristics under analysis: banana production (Factor 1), vegetative development (Factor 2), and cultivars and fruit diameter (Factor 3). The productive performance of the FHIA02 cultivar was homogeneous and inferior to that of the other cultivars, as seen in the two-way joining cluster analysis. Only the Grande Naine and IAC2001 cultivars are recommended for cultivation in the micro-region of Assis, São Paulo, with Grande Naine showing the largest fruit and, especially, the largest pulp diameter. cycles Farm dAlho d Alho d´Alho Palmital IAC IAC200 FHIA FHIA0 evaluated variables height perimeter harvest bunch hand finger peel analysis clustering twoway two way correspondence. correspondence . correspondence) 8963 89 63 89.63 Factor 1, 1 , 1) 2, 2 2) 3. 3 3) micro region especially IAC20 896 8 6 89.6 IAC2 89.

ABSTRACT: Purple-fleshed sweet potato is a tuber rich in anthocyanins, which are phenolic pigments that confer color and present bioactive capacity. Moreover, its incorporation into dairy products can bring technological and functional benefits. Thus, this article evaluated the impact of the addition of lyophilized purple-fleshed sweet potato powder- LP (0% - Control, 2%, and 4%) on the physical, chemical, and technological characteristics of Greek yogurt. LP showed in vitro antioxidant and bioactive capacity by DPPH (20.64 ± 1.61 μmol TE g-1), FRAP (112.93 ± 4.38 μmol TE g-1), total phenolic content (103.66 ± 3.35 mg GAE g-1), and total anthocyanin content (51.10 ± 1.87 mg cyanidin 3-glucoside 100 g-1). Additionally, the assays demonstrated that the LP has 16.82 ± 0.63% of resistant starch. The fortification of Greek yogurt with LP reduced the syneresis, indicating that LP increased the water retention capacity. LP also inhibited the post-acidification process, increasing apparent viscosity, hardness, and gumminess, and promoting a stable pink coloration throughout the storage (P < 0.05). At the 4% level, the yogurt was firmer and showed greater chewiness, which is highly desirable for Greek yogurt. Therefore, the results suggested that lyophilized purple-fleshed sweet potato powder is a potential multifunctional natural ingredient. ABSTRACT Purplefleshed Purple fleshed anthocyanins Moreover benefits Thus purplefleshed purple 0% 0 (0 Control 2 2% 4 physical chemical 20.64 2064 20 64 (20.6 161 1 61 1.6 g1, g1 g , g-1) 112.93 11293 112 93 (112.9 438 38 4.3 103.66 10366 103 66 (103.6 335 3 35 3.3 51.10 5110 51 10 (51.1 187 87 1.8 3glucoside glucoside g1. . Additionally 1682 16 82 16.8 063 63 0.63 starch syneresis postacidification post acidification process viscosity hardness gumminess P 0.05. 005 0.05 05 0.05) level chewiness Therefore ingredient ( 20.6 206 6 (20. 1. g-1 112.9 1129 11 9 (112. 43 4. 103.6 1036 (103. 33 3. 51.1 511 5 (51. 18 8 168 16. 06 0.6 00 0.0 20. (20 g- 112. (112 103. (103 51. (51 0. (2 (11 (10 (5 (1

RESUMO: Batata-doce de polpa roxa é um tubérculo rico em antocianinas, as quais são pigmentos fenólicos que conferem cor e apresentam capacidade bioativa. Além disso, sua incorporação em produtos lácteos pode trazer benefícios tecnológicos e funcionais. Desta forma, este estudo teve como objetivo avaliar o impacto da adição de pó liofilizado de batata-doce de polpa roxa - LP (0% - Controle, 2% e 4%) nas características físicas, químicas e tecnológicas de iogurte tipo Grego. LP apresentou capacidade antioxidante e bioativa in vitro para DPPH (20.64 ± 1.61 μmol TE g-1), FRAP (112.93 ± 4.38 μmol TE g-1), teor de compostos fenólicos totais (103.66 ± 3.35 mg GAE g-1) e teor de antocianinas totais (51.10 ± 1.87 mg cyanidin 3-glucoside 100 g-1). Adicionalmente, os ensaios demonstraram que o LP possui 16,82 ± 0,63% de amido resistente. A fortificação do iogurte Grego com LP reduziu a sinérese, indicando que LP aumentou a capacidade de retenção de água, LP também inibiu o processo pós-acidificação, aumentando a viscosidade aparente, a dureza e a gomosidade, e promovendo uma coloração rosa estável durante todo o armazenamento (P < 0,05). No nível de 4%, o iogurte apresentou-se mais firme e com maior mastigabilidade, o que é altamente desejável para o iogurte tipo Grego. Assim, os resultados sugerem que o pó liofilizado de batata-doce de polpa roxa é um potencial ingrediente natural multifuncional. RESUMO Batatadoce Batata doce disso funcionais forma batatadoce batata 0% 0 (0 Controle 2 4% 4 físicas 20.64 2064 20 64 (20.6 161 1 61 1.6 g1, g1 g , 112.93 11293 112 93 (112.9 438 38 4.3 103.66 10366 103 66 (103.6 335 3 35 3.3 g-1 51.10 5110 51 10 (51.1 187 87 1.8 3glucoside glucoside g1. . Adicionalmente 1682 16 82 16,8 063 63 0,63 resistente sinérese água pósacidificação, pósacidificação pós acidificação, acidificação pós-acidificação aparente gomosidade P 0,05. 005 0,05 05 0,05) apresentouse se mastigabilidade Assim multifuncional ( 20.6 206 6 (20. 1. 112.9 1129 11 9 (112. 43 4. 103.6 1036 (103. 33 3. g- 51.1 511 5 (51. 18 8 168 16, 06 0,6 00 0,0 20. (20 112. (112 103. (103 51. (51 0, (2 (11 (10 (5 (1

ABSTRACT The objective of this study was to evaluate the effects of prebiotics, alone and in combination with antibiotics, on broiler performance, carcass yield, intestinal permeability, and intestinal morphometry. A total of 1440 day-old male Cobb 500 chicks were distributed in a completely randomized design, with four treatments and 10 replications per treatment. The treatments used were a basal diet without prebiotics and antibiotics (BD), a basal diet with antibiotics (AB), a basal diet with prebiotics (PRE), and a basal diet with antibiotics and prebiotics (AB+PRE). To perform the treatment, the antibiotic zinc bacitracin and a prebiotic of S. cerevisiae-derived mannan-oligosaccharides and beta-glucans were used. The treatments did not affect the performance of birds from 1 to 7 and 1 to 21 days of age. From 1 to 35 and 1 to 42 days, birds fed the AB+PRE treatment had higher feed intake than those fed the AB treatment. From 1 to 35 days, birds fed the AB+PRE treatment showed a worse feed conversion ratio. However, there was no difference in carcass yield between treatments. A higher number of goblet cells was observed in the duodenum and ileum of birds fed AB+PRE, but lower counts were obtained in birds fed AB. Intestinal histomorphometry displayed similar responses between both ages regarding antibiotic and prebiotic treatments, except in the ileum at 42 days, in which the antibiotic elicited a better response. Intestinal permeability analysis showed no significant difference between diets. The use of prebiotics, alone or in combination with antibiotics, does not improve the performance of broilers. morphometry 144 dayold day old 50 design BD, BD , (BD) AB, (AB) PRE, PRE (PRE) AB+PRE. ABPRE . (AB+PRE) S cerevisiaederived cerevisiae derived mannanoligosaccharides mannan oligosaccharides betaglucans beta glucans 2 age 3 4 ratio However response diets broilers 14 5 (BD (AB (PRE (AB+PRE

ABSTRACT Purpose: to carry out a pilot study applying the Reading and Language Together - Brazil program (RELATo-Brazil), targeting the development of reading and oral language abilities, aiming to improve phonological awareness skills and vocabulary knowledge in the school context. Methods: 9 children of both genders, aged between 8 and 10, enrolled in the second year of elementary school at a private school in the city of Florianópolis, SC, Brazil, joined in the study. The program was applied within three phases: Phase 1 - pre-intervention, with reading and vocabulary assessment tests; Phase 2 - intervention with sessions of different types, A, B, C and Phase 3 - post-intervention, which consisted of the subjects' reassessment, using the same tests applied in Phase 1. The data were stored in Microsoft Excel spreadsheets and exported for statistical analysis in the IBM SPSS software, the 25th version. The significance level considered was p ≤ 0.05. Results: following 21 intervention sessions, a better performance among the participants was observed when comparing the results of the pre-intervention and post-intervention phases. There is a statistically considerable difference in the percentage distributions of the conceptual vocabulary fields for Furniture and utensils (p=0.028), Places (p=0.009) and Shapes and colors (p=0.047). The reading rate did not show a statistical difference, but the children performed better in reading time and accuracy after the intervention. Conclusion: the data suggest that training in phoneme awareness and oral language and linking them in the context of authentic book reading are effective strategies for improving both reading and oral language skills. Purpose RELAToBrazil, RELAToBrazil RELATo , (RELATo-Brazil) abilities Methods genders 10 Florianópolis SC phases preintervention, preintervention pre intervention, types A B postintervention, postintervention post subjects reassessment software th version 005 0 05 0.05 Results p=0.028, p0028 p=0.028 028 (p=0.028) p=0.009 p0009 009 (p=0.009 p=0.047. p0047 p=0.047 . 047 (p=0.047) Conclusion (RELATo-Brazil 00 0.0 p002 p=0.02 02 (p=0.028 p=0.00 p000 (p=0.00 p004 p=0.04 04 (p=0.047 0. p00 p=0.0 (p=0.02 (p=0.0 (p=0.04 p0 p=0. (p=0. p=0 (p=0 p= (p= (p

RESUMO Objetivo: realizar um estudo piloto, aplicando o programa Reading and Language Together - Brazil (RELATo-Brazil), com vistas a incentivar o desenvolvimento da leitura e da linguagem oral por meio do trabalho com as habilidades de consciência fonológica e o aprimoramento do vocabulário no contexto escolar. Métodos: participaram do estudo nove crianças, de ambos os gêneros, com idade entre 8 e 10 anos, matriculadas no terceiro ano do ensino fundamental de uma escola particular do município de Florianópolis, Santa Catarina. O programa foi aplicado em três fases: Fase 1- pré-intervenção, com provas de avaliação da leitura e vocabulário, Fase 2 - intervenção com sessões de diferentes tipos. Fase 3 - pós-intervenção, que constou da reavaliação dos sujeitos, utilizando a mesmas provas aplicadas na Fase 1. Os dados foram armazenados em planilhas do programa Microsoft Excel e exportados para análise estatística no software IBM SPSS versão 25. O nível de significância considerado foi de p≤0,05. Resultados: após 21 sessões de intervenção, constatou-se melhor desempenho dos participantes ao comparar os resultados das fases pré-intervenção e pós-intervenção. Há diferença estatisticamente significativa nas distribuições percentuais dos campos conceituais do vocabulário para Móveis e utensílios (p=0,028), Locais (p=0,009) e Formas e cores (p=0,047). A taxa de leitura não mostrou diferença estatística, porém melhor desempenho das crianças em tempo e acurácia da leitura após a intervenção. Conclusão: os dados sugerem que treinar a consciência fonêmica, a linguagem oral e a ligação entre as duas no contexto da leitura real de livros pode ser uma intervenção eficaz na leitura, além de promover competências de linguagem oral. Objetivo piloto RELAToBrazil, RELAToBrazil RELATo , (RELATo-Brazil) escolar Métodos gêneros 1 anos Florianópolis Catarina préintervenção, préintervenção pré tipos pósintervenção, pósintervenção pós pós-intervenção sujeitos 25 p005 p 0 05 p≤0,05 Resultados constatouse constatou se pósintervenção. p=0,028, p0028 p=0,028 028 (p=0,028) p=0,009 p0009 009 (p=0,009 p=0,047. p0047 p=0,047 . 047 (p=0,047) Conclusão fonêmica (RELATo-Brazil p00 p≤0,0 p002 p=0,02 02 (p=0,028 p=0,00 p000 00 (p=0,00 p004 p=0,04 04 (p=0,047 p0 p≤0, p=0,0 (p=0,02 (p=0,0 (p=0,04 p≤0 p=0, (p=0, p≤ p=0 (p=0 p= (p= (p

Abstract Biomarkers of environmental contamination have been frequently used in the assessment of marine ecosystem quality because they provide quantitative measures of biological changes in organisms exposed to pollutants such as hydrocarbons from oil spills. Polychaetes have been tested as sentinel organisms of marine environmental health because they are abundant taxa of benthic assemblages and their sedentary lifestyle ensures chronic exposure to toxins in impacted areas. In this study, we evaluated whether the polychaete Scolelepis goodbodyi can be used as a reliable sentinel species for exposure to polycyclic aromatic hydrocarbons (PAHs) from an experimental diesel spill in a dissipative sand beach in the Southern Atlantic. The design used in this study comprised replicated control and diesel impact sites sampled four times (one, two, four and seven days) before and after the impact. Total PAH levels reached 114.0 ng g -1 in the impact site one day after the diesel spill; however, the pattern of biomarker responses in S. goodbodyi was primarily influenced by spatial variation rather than being attributed to the simulated diesel spill. The apparent absence of a contaminating effect may be linked to the low levels of the toxins retained in the sediment after the spill was simulated to elicit a response or to the presence of an efficient repair system within the organism. Furthermore, the sediments tested in this study, composed of sandy fractions, had a low capacity to concentrate PAHs after the simulated diesel spill, which may have contributed to the low significant changes in their biomarker activities. Based on our results, further studies testing other Polychaeta species and simulating oil spills in sedimentary environments composed of fine sediments such as salt marshes and mangroves may help produce evidence on more effective biomarker responses in these organisms. areas (PAHs Atlantic one, (one two days 1140 114 0 114. 1 - however S organism Furthermore fractions activities results 11

Abstract This study analyzed the construction process of the chosen name and related aspects among transgender individuals from a medium-sized municipality in southern Brazil. Qualitative data from a descriptive and exploratory mixed-method study was used. Self-declared trans people aged 18 or over were included. Three focus groups were conducted with ten participants. These individuals, aged between 19 and 52 years, have a documented chosen name, but half have not yet changed their civil records. The content analysis technique produced two categories: “construction of the chosen name as an instrument for validating the transgender identity” and “impacts of the construction of the chosen name on self-acceptance and on family and social relationships.” The results reinforce the importance of respecting the name as a way of contributing to the gender affirmation of transgender individuals. The name was linked to the experiences, individuality and values of these individuals. Its proper use, much more than respect for legislation, meant respect for the different possibilities of human existence. It is expected that the results presented can be used as a tool for recognizing the issues and nuances that permeate the construction of the chosen name and contribute to identity validation. mediumsized medium sized Brazil mixedmethod mixed method Selfdeclared Self declared 1 included participants 5 years records categories impacts selfacceptance self acceptance relationships. relationships experiences use legislation existence validation

Resumo O trabalho analisou o processo de construção do nome social e aspectos relacionados em pessoas trans de um município de porte médio do Sul do Brasil. Foram utilizados dados qualitativos de um estudo de método misto descritivo e exploratório. Incluíram-se pessoas autodeclaradas trans com 18 anos ou mais. Foram conduzidos três grupos focais com dez participantes. Estes, com idade variando entre 19 e 52 anos, apresentam nome social documentado, porém metade ainda não fez a alteração nos registros civis. A técnica da análise de conteúdo produziu duas categorias: “construção do nome social como instrumento de validação da identidade trans” e “impactos da construção do nome social na autoaceitação e nas relações familiares e sociais”. Os resultados reforçam a importância do respeito ao nome como forma de contribuição para a afirmação de gênero de sujeitos trans. O nome esteve atrelado às suas vivências, individualidades e valores. Seu uso adequado, muito mais do que o cumprimento de uma legislação, significou respeito às possibilidades diversas da existência humana. Espera-se que os resultados apresentados possam ser utilizados como ferramenta de reconhecimento acerca das questões e nuances que permeiam a construção do nome social, bem como contribuam para a validação identitária. Brasil exploratório Incluíramse Incluíram se 1 participantes Estes 5 documentado civis categorias impactos sociais. sociais . sociais” vivências valores adequado legislação humana Esperase Espera identitária