Pesquisa | SciELO

This study sought to understand the perceptions of civil servants working in a federal higher education institution about working in collaboration with the city council in the development of care activities during the Covid-19 pandemic. We conducted a qualitative study based on interviews with 15 professionals. The data were analyzed using content analysis and the software Iramuteq. Two core thematic categories emerged from the analysis, each with their subcategories: feelings regarding the temporary displacement of the function; and understanding of institutional posture towards activities. The findings show that, despite protective factors and motivation for the work undertaken, critical moments involving public health caused emotional damage to the professionals and that seeking help for mental health problems should always be encouraged and support should be on hand as and when needed.

O artigo buscou compreender a percepção de servidores públicos de uma instituição federal de ensino superior acerca do trabalho realizado em colaboração com a prefeitura municipal, na atuação assistencial durante a pandemia de Covid-19. Trata-se de uma pesquisa com abordagem qualitativa que realizou entrevistas com 15 profissionais. Na análise dos dados, utilizou-se Análise de Conteúdo, apoiada pelo software Iramuteq, e deles emergiram duas categorias temáticas: “Sentimento em relação ao deslocamento momentâneo da função” e “Compreensão sobre postura institucional para que as atividades acontecessem”, cada uma com suas subcategorias. O estudo aponta que, mesmo com fatores de proteção e motivação para o trabalho realizado, os momentos críticos que envolveram a saúde pública causaram danos emocionais nos profissionais que atuaram nessas circunstâncias e a busca por apoio psicológico deve ser sempre encorajada e disponibilizada em caso de necessidade.

El artículo buscó comprender la percepción de servidores públicos de una institución federal de enseñanza superior sobre el trabajo realizado en colaboración con la municipalidad, en la actuación asistencial durante la pandemia de Covid-19. Investigación con abordaje cualitativo y entrevista a 15 profesionales. En el análisis de los datos se utilizó el Análisis de Contenido apoyado por el Software Iramuteq y de ellos surgieron dos categorías temáticas: Sentimiento con relación al desplazamiento momentáneo de la función y Comprensión sobre la postura institucional para que las actividades sucedieran, cada una con sus subcategorías. El estudio señala que incluso con factores de protección y motivación para el trabajo realizado, los momentos críticos que envolvieron la salud pública causaron daños emocionales en los profesionales que actuaron y la búsqueda de apoyo psicológico siempre debe incentivarse y ponerse a disposición en caso de necesidad.

https://doi.org/10.1590/interface.230530

Cryptococcoma hipofisiario: un extraño caso de hipofisitis granulomatosa secundaria
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Rotondaro, Julio C.

; Bettaza, Celeste

; Clifton Goldney, Dolores

; Martinez, Gabriela

; Paz, Leonardo

; Gazzi, Carla

; González Abbati, Santiago

; Tarnogol, Mariano

; Miguel, Silvia

; Lungman, Ariel

Medicina (Buenos Aires)

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Dez 2023, Volume 83 Nº 6 Páginas 1007 - 1012

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen La hipofisitis es una afección con baja incidencia y prevalencia. Asimismo, las infecciones profundas por hongos en pacientes inmunocompetentes también re presentan un fenómeno infrecuente. Más raro aún es el caso que se describe a continuación, en donde se conjugan estos dos elementos mencionados, a saber: cryptococcoma hipofisario o hipofisitis granulomatosa causado por dicho patógeno en un huésped sin altera ción de la respuesta inmune. Luego de una búsqueda realizada en PubMed, existen limitados casos en la literatura médica de hipofisitis granulomatosa por Cryptococcus spp., que simuló por manifestaciones clínicas e imagenológicas un macro adenoma hipofisario. No encontramos informes en los que no haya evidencia de afectación del tejido meníngeo. La etiología micótica está escasamente descrita en las guías de referencia para hipofisitis y creemos que Cryp tococcus spp. debe ser tenido en cuenta en el diagnóstico diferencial de las hipofisitis granulomatosas secundarias dado que es un patógeno ubicuo y el tratamiento es sustancialmente diferente a otras entidades. Cobra mayor relevancia ante la tendencia actual al uso de glucocorticoides sistémicos a altas dosis para el tratamiento de la hipofisitis, que podría haber gene rado mayor daño de no haberse hecho el diagnóstico correcto.

Abstract Hypophysitis is a pathology with low incidence and prevalence. Likewise, deep fungal infections in immuno competent patients also represent a rare phenomenon. Even rarer is the case described below, where these two mentioned elements are combined, namely: pituitary cryptococcoma or granulomatous hypophysitis caused by said pathogen in a host without altered immune response. After research in PubMed, there are limited cases in the medical literature of granulomatous hypophysitis caused by Cryptococcus spp., which simulated a pituitary macroadenoma by clinical and imaging manifestations. We did not find reports in which there is no evidence of involvement of the meningeal tissue. The fungal etiology is scarcely described in the reference guidelines for hypophysitis and we believe that Cryptococcus spp. it should be taken into account in the differential diagnosis of secondary granuloma tous hypophysitis since it is a ubiquitous pathogen and the treatment is substantially different from other entities. It becomes more relevant given the current trend towards the use of high-dose systemic glucocorticoids for the treatment of hypophysitis, which could have generated greater damage if the correct diagnosis had not been made.

Religiosity and Spirituality: The Relationship Between Psychosocial Factors and Cardiovascular Health
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Cruz, José Icaro Nunes

; Cardozo, Adelle Cristine Lima

; Melo, Enaldo Vieira de

; Lira, Juliana Maria Chianca

; Santos, Giulia Vieira

; Salazar, Gabriela de Oliveira

; Oliveira, Bruna

; Reis, Mariano César de Souza

; Lima, Diego Maldini Borba de

; Soares, Philipi Santos

; Sousa, Antônio Carlos Sobral

; Oliveira, Joselina Luzia Menezes

International Journal of Cardiovascular Sciences

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2023, Volume 36 elocation e20220176

Resumo: En | Texto: En | PDF: En

Abstract Background: Religiosity and Spirituality (R/S), despite being different entities, are multidimensional constructs, whose influence on cardiovascular health has been increasingly studied in recent decades. Objectives: To discriminate patients into subgroups according to R/S levels, in order to compare them regarding the distribution of cardiovascular comorbidities and clinical events. Methods: This is an observational, cross-sectional, analytical study. Two R/S scales were applied to a sample of patients seen at cardiology outpatient clinics. A cluster analysis was used to discriminate individuals into subgroups regarding R/S levels, which were subsequently compared regarding the frequencies of clinical variables related to cardiovascular health. A significance level of 5% was set for the statistical tests. Results: The sample included 237 patients with a mean age of 60.8 years (±10.7), of which 132 were female (55.7%). Cluster analysis (C) distinguished two groups: C1, with lower levels of R/S, and C2, with higher levels of R/S (p<0.001). C2 had a lower frequency of alcohol consumption (29.5% vs. 76.0%; p<0.001), smoking (12.9% vs. 51.0%; p<0.001), systemic arterial hypertension (SAH — 65.5% vs. 82.3%; p=0.005), dyslipidemia (58.3% vs. 77.1%; p=0.003), chronic coronary syndrome (36.7% vs. 58.3%; p=0.001), and prior cardiovascular events (15.8% vs. 36.5%; p<0.001) when compared to C1. There was also a higher frequency of females in C2 (82.0% vs. 17.7%; p<0.001). Conclusions: A better cardiovascular morbidity profile was observed in the group of patients with higher R/S levels, suggesting a probable positive relationship between R/S and cardiovascular health.

https://doi.org/10.36660/ijcs.20220176

Influencia de la técnica de muestreo en los resultados microbiológicos obtenidos de endoscopios flexibles reprocesados
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Cerna-Cardona, Jony

; Campos-Jiménez, Rachel

; Bello-López, Juan Manuel

; Loyola-Cruz, Miguel Ángel

; Sosa-Hernández, Óscar

; Cruz-Cruz, Clemente

; Durán-Manuel, Emilio Mariano

; Ibáñez-Cervantes, Gabriela

Endoscopia

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Dez 2022, Volume 34 Nº 4 Páginas 103 - 110

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Introduction and objective: This work describes the importance of adopting effective methods of sampling reprocessed endoscopes for the detection of microorganisms adhered to the internal walls of these devices. The objective of this work is to compare the rate of bacterial contamination of the channels of reprocessed endoscopes, through irrigation water culture and that obtained after brushing the channels. A secondary objective was to determine the antimicrobial susceptibility of the pathogens isolated in both methods. Method: A total of 26 endoscopes (duodenoscopes, gastroscopes, colonoscopes, bronchoscopes and echoendoscopes) were subjected to automatic cleaning and reprocessing before use in patients at Hospital Juárez de México. Cleaned and reprocessed endoscopes were simultaneously subjected to two bacteriological test methods based on no brushing (method A) and brushing (method B) of the channels. Bacterial strains isolated after carcass culture were identified to genus and species level and subjected to antimicrobial resistance testing. Results: Method A revealed zero rate of residual bacterial contamination in both channels (suction and working). The microbiological results obtained by method B revealed bacterial contamination rates of 30.7%. Bacterial strains belonging to the families Enterobacteriaceae, Bacillaceae, Pseudomonadaceae, Micrococcaceae and Staphylococcaceae were identified. High rates of antimicrobial resistance were detected against 12 tested antibiotics from eight different families. Conclusion: Implementation of microbiological surveillance of endoscope reprocessing with modified sampling methods is necessary to prevent healthcare-associated infections from endoscopic procedures.

Resumen Introducción y objetivo: Este trabajo describe la importancia de adoptar métodos efectivos de muestreo de endoscopios reprocesados para la detección de microorganismos adheridos a las paredes internas de estos dispositivos. El objetivo de este trabajo es comparar la tasa de contaminación bacteriana de los canales de endoscopios reprocesados, mediante cultivo del agua de irrigación y la obtenida posterior a un cepillado de los canales. Un objetivo secundario fue determinar la sensibilidad antimicrobiana de los patógenos aislados en ambos métodos. Método: Un total de 26 endoscopios (duodenoscopios, gastroscopios, colonoscopios, broncoscopios y ecoendoscopios) fueron sometidos a limpieza y reprocesamiento automático antes de su uso en pacientes del Hospital Juárez de México. Los endoscopios limpios y reprocesados se sometieron simultáneamente a dos métodos de prueba bacteriológica basados en ausencia de cepillado (método A) y cepillado (método B) de los canales. Las cepas bacterianas aisladas después del cultivo de canales se identificaron a nivel de género y especie y se sometieron a pruebas de resistencia antimicrobiana. Resultados: El método A reveló cero tasa de contaminación bacteriana residual en ambos canales (succión y trabajo). Los resultados microbiológicos obtenidos por el método B revelaron tasas de contaminación bacteriana del 30.7%. Se identificaron cepas bacterianas pertenecientes a las familias Enterobacteriaceae, Bacillaceae, Pseudomonadaceae, Micrococcaceae y Staphylococcaceae. Se detectaron altas tasas de resistencia antimicrobiana contra 12 antibióticos probados de ocho familias diferentes. Conclusión: La implementación de la vigilancia microbiológica del reprocesamiento de endoscopios con métodos modificados de muestreo es necesaria para prevenir infecciones asociadas a la atención de la salud por procedimientos endoscópicos.

https://doi.org/10.24875/end.23000009

Taller de concordancia en la evaluación de imágenes capilaroscópicas
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Izaguirre, Pía

; Romanini, Félix Enrique

; Mamani, Marta

; Montoya, Sandra Fabiana

; Toibar, Javier

; Catalán Pellet, Santiago

; Sirera, Ana Irene

; Dueñas, Lelly

; Cucchiaro, Natalia

; Cousseau, Mario

; Orellana González, Carlos Alberto

; Yucra, Demelza

; Tralice, Elda Rossella

; Raschia, Alan Juan

; Molina, María Josefina

; Lencia, María Verónica

; Espasa, Gabriela Vanesa

; Linarez Martínez, Miguel Alex

; Castro Coello, Vanesa Viviana

; García, Aldo

; Velloso, María Sofía

; González, Paula Alejandra

; German, Noelia Fernanda

; Conti, Silvana

; Savy, Florencia

; Morbiducci, Julieta Silvana

; Rivero, Mariano

; Saavedra, Leandro José

; Sabelli, Mirtha Rosa

; Rebak, Jonathan Eliseo

; Rojas Tessel, Romina

; Sorrentino, Laura

; Gervasoni, Viviana Lorena

; Marcantoni, María Beatriz

; Gervilla de Cafrune, Ana Lía

; Got, Julio

; Sánchez, Gabriela

; Castorino, Gerardo

; García, Lucila

; Sansinanea, Pierina

; Tamborenea, María Natalia

Revista argentina de reumatología

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Out 2022, Volume 33 Nº 4 Páginas 223 - 227

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen Introducción: la capilaroscopia es un método no invasivo que permite observar la microvasculatura en el área periungueal. Los resultados informados pueden ser altamente variables entre distintos observadores. A lo largo del tiempo surgieron métodos cuantitativos y semicuantitativos para mejorar la reproducibilidad. Objetivos: conocer el nivel de acuerdo intra e interobservador al informar los diferentes patrones capilaroscópicos en individuos con diferente nivel de entrenamiento. Materiales y métodos: estudio de corte transversal. Participaron médicos reumatólogos especialistas y en formación que habían realizado previamente un curso virtual de capacitación en capilaroscopia. Recibieron 40 imágenes capilaroscópicas proyectadas en una presentación de PowerPoint y debían responder a través de un cuestionario digital. Se evaluó la concordancia de respuestas intra e interobservador. Resultados: se encontró un alto nivel de concordancia global con un kappa 0,66 IC 95% (0,63-0,70) p<0,0000. También en otros grupos como reumatólogos en formación: kappa 0,65 IC 95% (0,60-0,71) p=0,0000, y médicos reumatólogos: kappa 0,67 IC 95% (0,62-0,72) p=0,0000. Conclusiones: el nivel de concordancia encontrado fue globalmente alto, independientemente del nivel de entrenamiento de los profesionales, y de ser o no reumatólogo. La concordancia fue superior cuando se comparó a quienes tenían más de 4 años de experiencia en la realización de videocapilaroscopia.

Abstract Introduction: videoapillaroscopy is a non-invasive method that allows the observation of the microvasculature in the periungual area. Reported results can be highly variable between different observers. Over time, quantitative and semi-quantitative methods emerged to improve reproducibility. Objetives: to know the level of intra and interobserver agreement when reporting the different capillaroscopic patterns in individuals with different levels of training. Materials and methods: cross section study. Specialist rheumatologists and those in training who had previously completed a virtual capillaroscopy training course participated. They received 40 capillaroscopic images projected in a PowerPoint presentation and had to issue their response through a digital questionnaire. Concordance of intra and interobserver responses was evaluated. Results: a high level of global agreement was found with a kappa 0.66 CI 95% (0.63-0.70) p<0.0000, also in other groups such as rheumatologists in training: kappa 0.65 CI 95% (0.60-0.71) p=0.0000, physicians rheumatologists: kappa 0.67 95% CI (0.62-0.72) p=0.0000. Conclusions: the level of agreement found was globally high, regardless of the level of training of the professionals, and whether or not they were a rheumatologist. Concordance was higher when compared to those who had more than 4 years of experience performing videocapillaroscopy.

https://doi.org/10.47196/rar.v33i4.709

Vacunación contra SARS-CoV-2 en pacientes con esclerosis sistémica en Argentina: preferencias, acceso y adherencia al plan de vacunación
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Mamani Ortega, María Lourdes

; Montoya, Sandra

; García, Mercedes

; Nitsche, Alejandro

; Molina, Josefina

; Pucci, Paula

; Pendon, Gisela

; López Meiller, María José

; Rojas Tessel, Romina

; Salvatierra, Gabriela Del Valle

; Yucra, Demelza

; Navarro, Sandra

; Zelaya, David

; Rivero, Mariano

; Sánchez, Gabriela

; Morbiducci, Julieta

; Secco, Anastasia

; Kerzberg, Eduardo

; Tamborenea, María Natalia

Revista argentina de reumatología

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Set 2022, Volume 33 Nº 3 Páginas 123 - 128

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen Introducción: en pacientes con enfermedades reumatológicas autoinmunes se recomienda la aplicación sistemática y secuencial de una serie de vacunas para la prevención de enfermedades transmisibles. El objetivo de este estudio fue estimar la proporción de pacientes con esclerosis sistémica (ES) que recibieron vacunación contra el coronavirus (SARS-CoV-2). Materiales y métodos: se envió una encuesta anónima por correo electrónico o contacto por WhatsApp desde mayo a septiembre de 2021, con preguntas para evaluar la adherencia al esquema de vacunación recomendado en pacientes con enfermedades reumatológicas, así como temores, preferencias y adherencia al esquema de vacunación contra el SARS-CoV-2. Resultados: se incluyeron 295 pacientes con ES. El 68,81% estaba vacunado contra el SARS-CoV-2 con al menos una dosis, de los cuales el 48,7% tenía dos dosis. El 84,75% refirió conversar con su médico sobre su esquema de vacunación general. Solo el 5,4% tenía las cuatro vacunas. El 93,56% manifestó voluntad de vacunarse contra el SARS-CoV-2; el 56,27% prefirió la vacuna Sputnik V. El 7,46% manifestó su voluntad de no vacunarse. Los factores que influyeron en la adherencia a la vacunación, con mayor frecuencia, fueron el miedo a contraer la infección por SARS-CoV-2 (86,1%) y las reacciones adversas (23,05%). Conclusiones: destacamos el hecho de que solo 6 meses después de que se dispusiera la vacunación contra el SARS-CoV-2, la mitad de los pacientes con ES tenía el esquema recomendado completo.

Abstract Introduction: in patients with autoimmune rheumatic diseases, the systematic and sequential application of a series of vaccines is recommended for the prevention of communicable diseases. The objective was to estimate the proportion of patients with systemic sclerosis (SSc) who received vaccination against coronavirus (SARS-CoV-2). Materials and methods: since may to september 2021, an anonymous survey was sent by email or messaging app, containing questions to assess adherence to the recommended vaccination schedule in patients with rheumatic diseases, as well as fears, preferences and adherence to vaccination schedule against SARS-CoV-2. Results: 295 patients with SSc were included. 68.81% were vaccinated for SARS-CoV-2 with at least one dose, 48.7% of this group had two doses. 84.75% reported talking to their doctor about their general vaccination schedule. Only 5.4% had all four vaccines. 93.56% expressed willingness to be vaccinated against SARS-CoV-2, 56.27% preferred the Sputnik V vaccine. 7.46% expressed their willingness to not be vaccinated. The factors that most frequently influenced adherence to vaccination were fear of contracting SARS-CoV-2 infection (86.1%) and adverse reactions (23.05%). Conclusions: we highlight the fact that only 6 months after vaccination against SARS-CoV-2 became available, half of the patients with SSc had the full recommended schedule.

https://doi.org/10.47196/rar.v33i3.663

Impact of COVID-19 on surgical residency training programs in Mexico City: The third victim of the pandemic. A resident’s perspective
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Oropeza-Aguilar, Mariano

; Cendejas-Gómez, José de Jesús

; Quiroz-Compeán, Alejandro

; Buerba, Gabriela A.

; Domínguez-Rosado, Ismael

; Mendez-Probst, Carlos E.

Cirugía y cirujanos

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Abr 2022, Volume 90 Nº 2 Páginas 165 - 171

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Objective: The aim of this study is to assess the perceptions of the impact of health-care disruption due to COVID-19 on the academic training and skills of surgical trainees. Material and Methods: We developed a 32-question survey assessing the clinical and surgical impact of COVID-19 on surgical training programs and proposals to compensate for the decrease in surgical education. We got 453 responses of surgical trainees in Mexico City. Results: Sixty-six percent of the respondents answered that their centers had converted to the exclusive attention of COVID-19 patients. Ninety-five percent reported a decrease in surgical skills learning and 91.8% reported a decrease to clinical exposure. On proposals, 75.6% reported that it is essential to take the necessary measures to recover the clinical and surgical milestones lost. In the binary logistic regression analysis, we found that the postgraduate year (≥ PG-Y3) was statistically significant factor (p ≤ 0.000) related to a favorable opinion to developing an academic contingency plan and postponing the end of the academic residency year. Conclusion: More than 90% of the survey respondents reported having been affected by COVID-19 mitigation strategies. Our data calls for urgent training adjustments by hospital and university program leaders to mitigate downstream educational repercussions.

Resumen Objetivo: Evaluar las percepciones del impacto de la interrupción de la atención médica por COVID-19 en la formación académica y las habilidades de los residentes quirúrgicos. Material y Métodos: Realizamos una encuesta de 32 preguntas, evaluando el impacto clínico y quirúrgico del COVID-19 en los programas de entrenamiento quirúrgico y propuestas para compensar la disminución de la educación quirúrgica. Obtuvimos 453 respuestas de residentes quirúrgicos en la Ciudad de México. Resultados: El 66% respondió que sus centros se convirtieron en atención exclusiva de pacientes con COVID-19. El 95% presentó una disminución en el aprendizaje de habilidades quirúrgicas y el 91. 8% presentó una disminución de la exposición clínica. El 75.6% consideró fundamental tomar las medidas necesarias para recuperar las destrezas clínicas perdidas. En el análisis de regresión logística binaria, encontramos que el año de posgrado (> PG-Y3) fue un factor estadísticamente significativo (p <0,000) relacionado con una opinión favorable para desarrollar un plan de contingencia académica y posponer el final del año de residencia académica. Conclusión: Más del 90% de los encuestados fueron afectados por las estrategias de mitigación de COVID-19. Nuestros datos exigen ajustes urgentes por parte de los líderes de programas de hospitales y universidades para mitigar las repercusiones educativas posteriores.

https://doi.org/10.24875/ciru.21000278 13 downloads

ELMO helmet for CPAP to treat COVID-19-related acute hypoxemic respiratory failure outside the ICU: aspects of/comments on its assembly and methodology
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Mazza, Mariano

; Fiorentino, Giuseppe

; Esquinas, Antonio M.

; Tomaz, Betina Santos

; Gomes, Gabriela Carvalho

; Lino, Juliana Arcanjo

; Menezes, David Guabiraba Abitbol de

; Soares, Jorge Barbosa

; Furtado, Vasco

; Soares Júnior, Luiz

; Farias, Maria do Socorro Quintino

; Lima, Debora Lilian Nascimento

; Pereira, Eanes Delgado Barros

; Holanda, Marcelo Alcantara

Jornal Brasileiro de Pneumologia

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2022, Volume 48 Nº 2 elocation e20220072

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

https://doi.org/10.36416/1806-3756/e20220072

Situación sociolaboral y Aislamiento Social, Preventivo y Obligatorio en Santiago del Estero, en el mes de abril de 2020*
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TORRES, Lucas Emanuel

; SAYAGO PERALTA, Eliana Gabriela

; CAJAL, Jorgelina Anahi

; GIMÉNEZ, Mariano Hernán

Trabajo y sociedad

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Dez 2020, Volume 21 Nº 35 Páginas 1 - 3

Resumo: Es | Texto: Es | PDF: Es

181 downloads

10.

Potencial deteriorante da microbiota mesófila, psicrotrófica, termodúrica e esporulada do leite cru
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Marioto, Louise Rodrigues Mariano

; Daniel, Gabriela Casarotto

; Gonzaga, Natalia

; Mareze, Juliana

; Tamanini, Ronaldo

; Beloti, Vanerli

Ciência Animal Brasileira

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2020, Volume 21 elocation e-44034

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Considerando os diversos problemas tecnológicos e econômicos que os microrganismos deteriorantes acarretam aos laticínios, o objetivo deste trabalho foi quantificar aeróbios mesófilos, psicrotróficos, termodúricos e esporulados no leite cru produzido na região Norte do Paraná e verificar a capacidade proteolítica e lipolítica desses microrganismos. Foram analisadas 20 amostras de leite cru e determinadas contagens de aeróbios mesófilos, psicrotróficos, termodúricos mesófilos, termodúricos psicrotróficos e esporos aeróbios e o perfil proteolítico e lipolítico de cada grupo. A contagem média de aeróbios mesófilos foi de 1,7x106UFC/mL acima do limite estabelecido pela Instrução Normativa 76 (3,0x105UFC/mL); 55% das amostras apresentaram contagens dentro dos padrões. As contagens de psicrotróficos variaram de 1,0x102 a 9,9x106UFC/mL e em 30% das amostras esse valor foi superior ao de mesófilos. A média de termodúricos mesófilos foi de 2,4x104UFC/mL, de termodúricos psicrotróficos 1,7x102UFC/mL e de esporos aeróbios foi de 5,0x101UFC/mL. Quanto ao perfil deteriorante, todos os grupos apresentaram atividade lipolítica e proteolítica elevadas, com predomínio de bactérias lipolíticas. Os valores encontrados indicam que o manejo higiênico-sanitário inadequado durante a ordenha e o resfriamento prolongado favorece a proliferação da microbiota deteriorante, que pode promover alterações significativas na qualidade e no rendimento do leite e seus derivados, mesmo após tratamento térmico.

Abstract Considering that technological problems and economic losses are basically caused by spoilage microorganisms on dairy industry, therefore the aim of this study was to quantify the mesophilic aerobic, psychrotrophic, thermoduric and aerobic spore-forming in raw milk produced in northern Paraná and also verify the proteolytic and lipolytic capacity of these microorganisms. Twenty samples of raw milk were analyzed and were determined mesophilic aerobic, psychrotrophic, thermoduric mesophilic, psychrotrophic thermoduric and aerobicspore-forming counts and proteolytic and lipolytic capacity from each group. The average counts of aerobic mesophilic were 1,79x106CFU/mL, above the limit established to Normative Instruction 76 (3,0x105CFU/mL); 55% samples had scores within the standards. Psychrotrophic counts ranged from 1,0x102 to 9,9x106CFU/mL and in 30% samples this count was higher than mesophilic. The average mesophilic thermoduric was 2,4x104CFU/mL, psychrotrophic thermoduric was 1,7x102CFU/mL and aerobic spore-forming was 5,0x101CFU/mL. As for the spoilage profile, all groups showed higher lipase and proteolytic activity, with a predominance of lipolytic bacteria. The values found indicates the inappropriate hygienic-sanitary management during milking and maintaining molk refrigerated for long periods until pasteurize, it can favorsthe proliferation of spoilage microbiota, which can promote significant changes in the quality and yield of milk and dairy products, even after the heat treatment.

https://doi.org/10.1590/1809-6891v21e-44034 1983 downloads

11.

Prebiotic effect of commercial saccharides on probiotic bacteria isolated from commercial products
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FIGUEROA-GONZÁLEZ, Ivonne

; RODRÍGUEZ-SERRANO, Gabriela

; GÓMEZ-RUIZ, Lorena

; GARCÍA-GARIBAY, Mariano

; CRUZ-GUERRERO, Alma

Food Science and Technology

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Set 2019, Volume 39 Nº 3 Páginas 747 - 753

Resumo: En | Texto: En | PDF: En

Abstract This study deals with the ability of probiotic bacteria to ferment prebiotics in vitro to estimate the prebiotic activity score and prebiotic index based on specific substrates and strains. Five probiotic bacteria were isolated from commercial products and their fermentation capability of three commercial prebiotics was assessed. Growth kinetics of probiotics showed that all of them were able to use the prebiotics as carbon sources. Mostly bacteria grew faster on Frutafit and Oligomate 55 than on lactulose. The prebiotic activity score and prebiotic index concepts were introduced to evaluate the performance of every probiotic with each prebiotic; accordingly, the highest values were for L. rhamnosus on Oligomate 55, while the worst according to these parameters was L. casei Shirota on Frutafit. Prebiotic index and prebiotic activity score describe well the selective growth of probiotics supported by prebiotics; therefore, they could be useful tools to define adequate combinations in colonic foods development.

https://doi.org/10.1590/fst.07318 2350 downloads

12.

Artritis reumatoidea asociada a colangitis biliar primaria bajo tratamiento con medicamentos biológicos: Reporte de 4 casos y puesta al día
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Jones, Xaviar Michael

; Montiel Bertone, Mariano

; Savio, Verónica Gabriela

; Werner, Marina Laura

; Strusberg, Ingrid

Revista argentina de reumatología

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Jun 2019, Volume 30 Nº 2 Páginas 20 - 23

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

The therapeutic approach of patients with two or more autoimmune diseases is quite a challenge, especially when the treatment of one of them, can precipitate the progression of the other. Even though the association of rheumatoid arthritis (RA) and primary biliary cholangitis (PBC) is rare; when both coexist, the use of methotrexate and other hepatotoxic drugs should be used with caution. With a most widespread indication of biologic disease-modifying antirheumatic drugs (bDMARDs) some reports of patients with RA and PBC treated with etanercept, infliximab, rituximab, tocilizumab and abatacept have been published. We report a case series that includes 4 patients with RA and PBC treated with bDMARDs. This is the first report to describe two cases in which golimumab was used to control RA and the second to report patients who received adalimumab and abatacept. Three cases of patients treated with rituximab have been published to date. None of the patients of our report suffered a progression of their PBC; matter in fact, two of them showed an improvement in their biochemical parameters. PBC symptoms did not get worse in any of the patients. On the contrary, laboratory parameters improved in two of the four patients. In conclusion, according to the literature reviewed and to our findings, the use of bDMARDs could be considered in RA patients with concomitant PBC.

El abordaje terapéutico de pacientes con dos o más enfermedades autoinmunes es un verdadero desafío, especialmente cuando el tratamiento enfocado en una de ellas podría precipitar la progresión de la otra. Si bien la asociación de artritis reumatoidea (AR) con colangitis biliar primaria (CBP) no es tan frecuente, cuando coexisten, la utilización de metotrexato u otras drogas hepatotóxicas debe decidirse con cautela. Con la indicación más generalizada de las drogas biológicas modificadoras del curso de la AR (DMARb) han aparecido algunos reportes de pacientes con AR y CBP tratados con etanercept, infliximab, rituximab, tocilizumab y abatacept. Presentamos una serie de casos de 4 pacientes con AR y CBP que fueron tratados con DMARb. Nuestro reporte sería el primero en describir dos casos con golimumab para controlar la AR y el segundo en reportar un caso con adalimumab y otro con abatacept. Con rituximab, ya existen tres casos publicados. En ninguno de los pacientes de nuestra serie empeoraron los síntomas de CBP y, al contrario, en dos de ellos hubo mejoría de los parámetros bioquímicos. En conclusión, según lo observado y lo reportado en la literatura, el uso DMARb podría ser considerado en el caso de pacientes con AR activa que además padecen CBP.

256 downloads

13.

Diálogos interculturales sobre territorios ancestrales en la provincia de Buenos Aires, Argentina
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Millán, Mirta Fabiana

; Chaparro, María Gabriela

; Mariano, Mercedes

Íconos. Revista de Ciencias Sociales

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Abr 2019, Nº 63 Páginas 161 - 184

Resumo: En Pt Es | Texto: En Pt Es | PDF: En | PDF: Pt | PDF: Es

Abstract The objective of this article is to make several ancestral territories in the Olavarría party (province of Buenos Aires, Argentina) visible, focusing on their socio-cultural continuity and space-time interaction based on an intercultural dialogue between the authors. For this purpose, we chose three archaeological sites that have been systematically analyzed and studied by researchers, to rethink them as ancient territories, which from the kimun (knowledge) of the Mapuche people, make other logics about space and time possible. This article is the result of a first experience of coproduction of knowledge that sought, through an exchange of indigenous and academic insights, to promote new knowledges about local identities, their continuity and reemergence. In order to accomplish this task, we worked with a qualitative methodology that articulated scientific production with oral testimonies and ancestral knowledge of members of contemporary indigenous communities.

Resumo Com o presente trabalho nos propusemos a visualizar diversos territórios ancestrais existentes no município de Olavarría (província de Buenos Aires, Argentina), em relação com a sua continuidade sociocultural e interação espaço-temporal, a partir de um diálogo intercultural entre as autoras. Por esse motivo, escolhemos três sítios arqueológicos que têm sido sistematicamente analisados e estudados por pesquisadores, para repensá-los como territórios milenares que permitem, desde o kimun (conhecimento) do povo mapuche, outras lógicas do espaço e do tempo. Este artigo é o resultado de uma primeira experiência de coprodução de saberes com a qual se buscou, através de um intercâmbio de conhecimentos indígenas e acadêmicos, promover outras experiências em torno de identidades locais, suas continuidades e ressurgimentos. Para isso, trabalhamos com uma metodología qualitativa que articulou a produção científica com as declarações orais e saberes ancestrais de membros de povos indígenas contemporâneos.

Resumen Con el presente trabajo nos propusimos visibilizar diversos territorios ancestrales existentes en el partido de Olavarría (provincia de Buenos Aires, Argentina), en relación con su continuidad sociocultural e interacción espacio-temporal a partir de un diálogo intercultural entre las autoras. Por ello, elegimos tres sitios arqueológicos que han sido sistemáticamente analizados y estudiados por investigadores, para repensarlos como territorios milenarios que posibilitan, desde el kimun (conocimiento) del pueblo mapuche, otras lógicas del espacio y del tiempo. Este artículo es resultado de una primera experiencia de coproducción de saberes con la que se buscó, mediante un intercambio de conocimientos indígenas y académicos, propiciar otros nuevos en torno de identidades locales, sus continuidades y reemergencias. Para ello, trabajamos con una metodología cualitativa que articuló la producción científica con los testimonios orales y saberes ancestrales de miembros de pueblos originarios contemporáneos.

https://doi.org/10.17141/iconos.63.2019.2951 37 downloads

14.

Frequency and factors associated with Toxoplasma gondii infection in pregnant women and their pets in Ilhéus, Bahia, Brazil
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Oliveira, Gabriela Mota Sena de

; Simões, Juçara Magalhães

; Schaer, Robert Eduard

; Freire, Songeli Menezes

; Nascimento, Roberto José Meyer

; Pinheiro, Adélia Maria Carvalho de Melo

; Carvalho, Silvia Maria Santos

; Mariano, Ana Paula Melo

; Carvalho, Rosely Cabral de

; Munhoz, Alexandre Dias

Revista da Sociedade Brasileira de Medicina Tropical

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2019, Volume 52 elocation e20190250

Resumo: En | Texto: En | PDF: En

Abstract INTRODUCTION: Toxoplasmosis is an asymptomatic disease that can lead to systemic disease in the fetus of pregnant women with primary infection. This study aimed to determine the prevalence of toxoplasmosis, associated factors, and correlation between the serology of pregnant women and their pets, in the municipality of Ilhéus, Bahia, Brazil. METHODS: This cross-sectional study was conducted in 196 pregnant women and their cats or dogs (n=89). Semi-structured interviews were conducted and serum samples from the pregnant women were tested to detect IgM and IgG antibodies against Toxoplasma gondii, and avidity tests were performed for IgM-positive samples. The serum collected from pets were tested for IgG antibodies, and IgM antibodies in cats. A non-conditional logistic regression analysis was performed to identify infection-associated factors. RESULTS: IgG and IgM antibodies were detected in 67.9% (133/196) and 1.5% (3/196) samples, respectively, for women with an avidity of over 60%. Age ≥ 25 and the presence of cats in the vicinity were found to be associated with infection, while the level of education and previous orientation toward prevention of toxoplasmosis were protective factors in pregnant women. IgG antibodies were detected in 46.1% (41/89) of the animals, and cats were found to be negative for IgM. For the animals, age ≥ 1 year was a factor associated with infection. There was no correlation between serology of the pregnant women and the animals (p=0.15). CONCLUSIONS: An elevated prevalence of toxoplasmosis was detected in the region. Therefore, the adoption of preventive measures by public healthcare bodies is recommended.

https://doi.org/10.1590/0037-8682-0250-2019 1024 downloads

15.

Titanium Biomimetically Coated With Hydroxyapatite, Silver Nitrate and Polycaprolactone, for Use In Biomaterials (Biomedicine)
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Ferreira, Carolina Cruz

; Sousa, Lucíola Lucena de

; Ricci, Virgilio Pereira

; Rigo, Eliana Cristina da Silva

; Ramos, Alfeu Saraiva

; Campos, Maria Gabriela Nogueira

; Mariano, Neide Aparecida

Materials Research

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2019, Volume 22 elocation e20190177

Resumo: En | Texto: En | PDF: En

Titanium is used in orthopedic and orthodontic implants because it has good corrosion resistance and excellent biocompatibility. Thus, studies seek to obtain a coating to improve the adhesion between the bone and the implant, by modifying the metal’s surface. The objective of this work was to biomimetically coat C.P. Ti with hydroxyapatite doped with silver nitrate, a component with antimicrobial properties, coating the metallic-ceramic composite with a polycaprolactone polymer film, which is known by generate improved implant-tissue interaction, and reducing postoperative complications from bacterial infections. The characterization of the material demonstrated the existence of the coating overall surface of the metallic substrate. The results obtained from the bacterial culture tests with Staphylococcus aureus showed that nitrate was effective in reducing the amount of live bacteria present in the supernatant, as well as those adhered to the surface of the material. In addition, the polymeric coating did not prevent the release of the bactericidal agent, not interfering in the effect there.

https://doi.org/10.1590/1980-5373-mr-2019-0177 617 downloads

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