Abstract Introduction: Mining is one of the main drivers of deforestation of tropical forests. This activity affects the storage of aboveground biomass of these ecosystems; therefore, their ability to contribute to the mitigation of global climate change. Objective: To assess the influence of soil properties on the aboveground biomass storage of post-mining forests in the Colombian Pacific. Methods: Plots were established in areas post-mining and with different successional ages (12-15 years, 30-35 years, and mature forest). The aboveground biomass and physicochemical parameters of the soil were measured. Results: An aboveground biomass of 15.58 t ha-1, 35.17 t ha-1, and 178.32 t ha-1 was recorded at 12-15 years, 30-35 years, and mature forests, respectively. The species with the highest biomass content in post-mining forests were Cespedesia spathulata and Clidemia septuplinervia. The aboveground biomass was positively correlated with organic matter (OM), calcium (Ca), magnesium (Mg), CICE, total nitrogen (N), and silt. In contrast, the relationship was negative with sand, aluminum (Al), and potassium (K) content. It was evidenced that the relationship between aboveground biomass and soils differed in each successional age. When evaluating the changes of aboveground biomass and soils in the succession, it was observed that the aboveground biomass and total N increased with the recovery time. At the same time, the P and K decreased with succession. On the other hand, the contents of OM, Mg, Al, Ca, and CICE showed curvilinear tendencies since they increased in the first stages and then decreased in the advanced successional stages. Conclusions: Aboveground biomass increases with forest recovery time in the study area. This increase is influenced by the presence of two dominant species shared among the investigated ecosystems and by the soil’s N, P, and K content.

Resumen Introducción: La minería es una de las principales causas de deforestación de los bosques tropicales. Esta actividad afecta el almacenamiento de biomasa aérea de estos ecosistemas; y, por tanto, su capacidad para contribuir a la mitigación del cambio climático global. Objetivo: Evaluar la influencia de las propiedades del suelo en el almacenamiento de la biomasa aérea de bosques post-minería del Pacífico colombiano. Métodos: Se establecieron parcelas en áreas post-minería con diferentes edades de sucesión (12-15 años, 30-35 años y bosque maduro). Se midió la biomasa aérea y parámetros fisicoquímicos del suelo. Resultados: Se registró una biomasa aérea de 15.58 t ha-1, 35.17 t ha-1 y 178.32 t ha-1 en 12-15 años, 30-35 años y bosque maduro, respectivamente. Las especies con mayor contenido de biomasa en los bosques post-minería fueron Cespedesia spathulata y Clidemia septuplinervia. La biomasa aérea se correlacionó positivamente con la materia orgánica (MO), calcio (Ca), magnesio (Mg), CICE, nitrógeno total (N) y limo. Por el contrario, la relación fue negativa con el contenido de arena, aluminio (Al) y potasio (K). Se evidenció que la relación entre la biomasa aérea y los suelos difería en cada edad sucesional. Al evaluar los cambios de la biomasa aérea y los suelos en la sucesión, se observó que la biomasa aérea y el N total aumentaron con el tiempo de recuperación. Al mismo tiempo, el P y el K disminuyeron con la sucesión. Por otro lado, los contenidos de OM, Mg, Al, Ca, y CICE mostraron tendencias curvilíneas ya que aumentaron en los primeros estadios y luego disminuyeron en los estadios sucesionales avanzados. Conclusiones: la biomasa aérea aumenta con el tiempo de recuperación del bosque en el área de estudio. Este incremento está influenciado por la presencia de dos especies dominantes compartidas entre los ecosistemas investigados y por el contenido de N, P y K del suelo.

ABSTRACT This study aimed to investigate the effects of different levels of vitamin D3 in broiler diets on performance and bone health. A total of 360 one-day old male Cobb500® broiler chicks were subjected to five treatments of different levels of vitamin D3 in diets during two rearing phases: 0 IU/kg in both phases; 625 IU/kg in starter and 500 IU/kg in grower phase (25% of commercial inclusion); 1,250 IU/kg in starter and 1,000 IU/kg in grower phase (50% of commercial inclusion); 1,875 IU/kg in starter and 1,500 IU/kg in grower phase (75% of commercial inclusion); and 2,500 IU/kg in starter and 2,000 IU/kg in grower phase (100% of commercial inclusion). The traits studied weekly were feed intake, body weight, feed conversion, and viability. At 21 and 35 days of age, tibiae and femurs were removed, dissected, and evaluated for dry matter (DM), ash (%MM), calcium (%Ca), phosphorus (%P) and breaking strength. At 35 days of age, the bones were subjected to histopathological analysis for macro- and microscopic morphological evaluation. Data were subjected to regression analysis, using α = 0.05. The variables of percentage %MM, %Ca, %P, and breaking strength experienced a positive linear effect up to the supplementation levels of approximately 25% of inclusion. The histopathological analysis found that the group that received the diet with 100% inclusion of vitamin D3 presented lesions compatible with osteopetrosis and tibial dyschondroplasia. The observed results showed that for isonutritive diets, reduced levels of vitamin D3 guaranteed performance during the evaluated period while the use of 100% of vitamin D3, as commonly used, can cause bone diseases and harm the welfare of broilers. D health 36 oneday one day Cobb500 Cobb phases IUkg IU kg 62 50 25 (25 inclusion) 1250 1 250 1,25 1000 000 1,00 50% (50 1875 875 1,87 1500 1,50 75% 75 (75 2500 2 2,50 2000 2,00 100 (100 . intake weight conversion viability 3 age removed dissected DM, DM , (DM) MM %MM (%MM) Ca %Ca (%Ca) %P P (%P macro evaluation 005 05 0.05 dyschondroplasia used broilers Cobb50 6 5 (2 125 1,2 00 1,0 (5 187 87 1,8 150 1,5 7 (7 2,5 200 2,0 10 (10 (DM (%MM (%Ca 0.0 Cobb5 ( 12 1, 18 8 15 2, 20 (1 0.

Abstract Cardiac amyloidosis (CA) is a progressive condition marked by the accumulation of amyloid fibrils in cardiac tissue, resulting in cardiac dysfunction and, ultimately, heart failure. Pyrophosphate scintigraphy has emerged as a promising tool for the early detection and monitoring of CA, providing valuable insights into the disease's extent and severity. This review examines the current use of pyrophosphate scintigraphy in distinguishing between different types of amyloidosis, assessing risk levels, and monitoring patients with CA. It underscores the clinical significance and prospects of this imaging technique. CA (CA tissue ultimately failure diseases disease s severity levels technique

Resumo O objetivo deste trabalho foi avaliar as propriedades físico-químicas da palha de café, do capim-elefante e da cana-de-açúcar isolados ou combinados entre si ou com compostos comerciais, bem como seu uso como substrato para a produção de mudas clonais de Coffea canephora. O experimento foi conduzido em duas etapas: uma para a caracterização físico-química dos substratos, e a outra para a avaliação do crescimento das mudas de café nesses substratos. Na primeira etapa, os tratamentos consistiram de palha de café (PC), capim-elefante (CE), cana-de-açúcar (CA), substrato comercial (SC), vermiculita (VM) e suas combinações. Na segunda etapa, avaliou-se, também, o substrato padrão (solo). A PC apresentou altas condutividade elétrica e densidade e baixos espaço de aeração e água disponível. A CA se destacou por seus menores pH e densidade e sua maior porosidade. A CA e o CE foram viáveis para a produção de mudas de café, sem diferir dos substratos comercial e padrão. Com exceção de PC+CE, PC+SC e CE+CA, os substratos combinados proporcionaram maior crescimento das mudas que os isolados. A PC foi viável como substrato apenas em combinação, principalmente com VM. A combinação de PC, CA e CE com o substrato padrão melhora a qualidade de mudas clonais de C. canephora. físicoquímicas físico químicas capimelefante capim elefante canadeaçúcar cana açúcar comerciais canephora etapas físicoquímica química etapa , (PC) CE, (CE) CA, (CA) SC, SC (SC) VM (VM combinações avaliouse, avaliouse avaliou se, avaliou-se também solo. solo . (solo) disponível porosidade PCCE PC+CE PCSC CECA CE+CA C (PC (CE (CA (SC (solo

Abstract The objective of this work was to evaluate the physicochemical properties of coffee husk, elephant grass, and sugarcane alone or combined with each other or with commercial compounds, as well as their use as substrate for the production of clonal Coffea canephora seedlings. The experiment was carried out in two stages: one for the physicochemical characterization of the substrates, and the other for evaluations of the growth of coffee seedlings in these substrates. In the first stage, the treatments consisted of coffee husk (CH), elephant grass (EG), sugarcane (SC), commercial substrate (CS), vermiculite (VM), and their combinations. In the second stage, the standard substrate (soil) was also evaluated. CH presented a high electrical conductivity and density and a low aeration space and available water. SC stood out for its lower pH and density and its greater porosity. SC and EG were viable for coffee seedling production, not differing from the commercial and standard substrates. With the exception of CH+EG, CH+CS, and EG+SC, the combined substrates allowed of a greater seedling growth than the isolated ones. CH was only viable as a substrate when combined, especially with VM. Combining CH, SC, and EG to the standard substrate improves the quality of clonal C. canephora seedlings. compounds stages stage , (CH) EG, (EG) (SC) CS, CS (CS) VM, VM (VM) combinations soil (soil evaluated water porosity CHEG CH+EG CHCS CH+CS EGSC EG+SC ones C (CH (EG (SC (CS (VM

RESUMO Há evidências de que as taxas de acúmulo de massa seca e nutrientes das cultivares mais precoces (determinadas) são mais rápidas do que aquelas de ciclo normal ou tardio (indeterminado). Objetivou-se quantificar a extração e exportação de nutrientes e o índice de colheita de cultivares de feijão-comum determinadas e indeterminadas. Utilizou-se delineamento em blocos casualizados, com quatro repetições e oito cultivares (BRS FC104, TAA Gol, BRS Estilo, BRSMG Uai, IPR Tuiuiú, BRSMG Marte, Pérola e TAA Dama). Foram determinados o acúmulo de massa seca e de nutrientes nos estádios vegetativo e reprodutivo, produtividade e exportação de nutrientes. No início do estádio vegetativo, as cultivares precoces TAA Gol e BRS FC104 (tipo I - determinadas) apresentaram maior acúmulo de massa seca e nutrientes do que as indeterminadas tipos II e III. No entanto, a partir de R7, não houve diferenças entre as cultivares nas extrações, bem como nas exportações de nutrientes e no índice de colheita. Foram exportados 33; 11; 17; 2,3; 2,2; e 1,5 kg t-1 de grão de N, P2O5, K2O, S, Mg e Ca, respectivamente. No caso dos micronutrients, foram 14; 3,7; 70; 13; e 34 g Mg-1 de grão de B, Cu, Fe, Mn e Zn. As cultivares determinadas TAA Gol e BRS FC104 atingiram o estádio R9 aos 77 dias e as indeterminadas aos 85 (BRSMG Marte), 90 (IPR Tuiuiú e BRSMG Uai) e 93 dias (TAA Dama e Pérola). BRS Estilo, BRS FC104 e TAA Gol apresentaram as menores produtividades (< 2.200 kg ha-1) e TAA Dama a maior produtividade (3.275 kg ha-1). (determinadas indeterminado. indeterminado . (indeterminado) Objetivouse Objetivou se feijãocomum feijão comum Utilizouse Utilizou casualizados FC Estilo Uai Marte Dama. Dama) reprodutivo FC10 tipo III entanto R7 R extrações 33 11 17 2,3 23 2 3 2,2 22 15 1 5 1, t1 t t- N P2O5 PO P O K2O KO K S Ca respectivamente micronutrients 14 3,7 37 7 70 13 Mg1 Mg- B Cu Fe Zn 8 , Marte) 9 Pérola. Pérola) < ( 2200 200 2.20 ha1 ha ha-1 3.275 3275 275 (3.27 ha1. (indeterminado FC1 2, P2O 3, 220 20 2.2 ha- 3.27 327 27 (3.2 2. 3.2 32 (3. 3. (3

ABSTRACT There is evidence that the dry mass and nutrient accumulation rates of earlier (determinate) cultivars are faster than those of normal or late cycle (indeterminate). This study aimed to quantify the nutrient extraction and export and calculate the harvest index of determinate and indeterminate common bean cultivars. A randomized block design was used, with four replicates and eight cultivars (BRS FC104, TAA Gol, BRS Estilo, BRSMG Uai, IPR Tuiuiú, BRSMG Marte, Pérola and TAA Dama). The accumulation of dry matter and nutrients at the vegetative and reproductive stages, grain yield and grain nutrient export were determined. The early cultivars TAA Gol and BRS FC104 (Type I - determinate) accumulated more dry matter and nutrients during the vegetative stage than the indeterminate types II and III. However, from R7, no differences were observed among the cultivars for extractions or nutrient exports and harvest index. The cultivars exported 33, 11, 17, 2.3, 2.2 and 1.5 kg t-1 of grain for N, P2O5, K2O, S, Mg and Ca, respectively. Micronutrient exports were 14, 3.7, 70, 13 and 34 g Mg-1 of grain for B, Cu, Fe, Mn and Zn, respectively. The TAA Gol and BRS FC104 determinate cultivars reached the R9 stage at 77 days and the indeterminate cultivars at 85 (BRSMG Marte), 90 (IPR Tuiuiú and BRSMG Uai) and 93 days (TAA Dama and Pérola). BRS Estilo, BRS FC104 and TAA Gol had the lowest grain yield (< 2,200 kg ha-1) and TAA Dama the highest grain yield (3,275 kg ha-1). (determinate indeterminate. . (indeterminate) used FC Estilo Uai Marte Dama. Dama) stages determined FC10 Type III However R7 R 33 11 17 23 2 3 2.3 22 2. 15 1 5 1. t1 t t- N P2O5 PO P O K2O KO K S Ca respectively 14 37 7 3.7 70 Mg1 Mg- B Cu Fe Zn 8 , Marte) 9 Pérola. Pérola) < ( 2200 200 2,20 ha1 ha ha-1 3,275 3275 275 (3,27 ha1. (indeterminate FC1 P2O 3. 220 20 2,2 ha- 3,27 327 27 (3,2 2, 3,2 32 (3, 3, (3

ABSTRACT Objectives: To assess the molecular profile of follicular cell-derived thyroid carcinomas (FCDTCs) and correlate the identified mutations with the clinical and pathological features of the affected patients. Materials and methods: Cross-sectional study of tumor samples from 100 adult patients diagnosed with FCDTC between 2010 and 2019. The patients’ clinical and pathological data were collected. Genomic DNA was extracted from formalin-fixed, paraffin-embedded (FFPE) tumors using the ReliaPrep FFPE gDNA Miniprep System. Genotyping of target genomic regions (KRAS, NRAS, BRAF, EGFR, and PIK3CA) was performed using the AmpliSeq panel, while sequencing was performed on the iSeq 100 platform. Results: The patients’ mean age was 39 years. In all, 82% of the tumors were classic papillary thyroid carcinomas. Overall, 54 (54%) tumor samples yielded satisfactory results on next-generation sequencing (NGS), of which 31 harbored mutations. BRAF gene mutations were the most frequent, with the BRAFV600E mutation present in 10 tumors. Seven tumors had BRAFNON-V600E mutations not previously described in FCDTCs (G464E, G464R, G466E, S467L, G469E, G596D, and the T599Ifs*10 deletion) but described in other types of cancer (i.e., skin/melanoma, lung, colorectal, and others). One tumor had a previously reported BRAFA598V mutation. EGFR gene mutations were found in 16 (29%) and KRAS or NRAS alterations in 8 (14%) of the 54 tumors analyzed. Conclusion: We described herein seven non-hotspot/novel variants in the BRAF gene, highlighting their potential role in expanding our understanding of FCDTC genetics. Objectives cellderived cell derived (FCDTCs methods Crosssectional Cross sectional 201 2019 collected formalinfixed, formalinfixed formalin fixed, fixed formalin-fixed paraffinembedded paraffin embedded (FFPE System KRAS, (KRAS PIK3CA PIKCA PIK CA panel platform Results 3 years all 82 Overall 5 54% (54% nextgeneration next generation NGS, NGS , (NGS) frequent BRAFVE BRAFV E 1 BRAFNONV600E BRAFNONVE BRAFNON V600E V G464E, G464E GE G (G464E G464R GR R G466E S467L SL S L G469E G596D GD D T599Ifs10 TIfs T599Ifs T Ifs T599Ifs*1 deletion i.e., ie i e (i.e. skinmelanoma skin melanoma skin/melanoma lung colorectal others. others . others) BRAFAV BRAFA 29% 29 (29% 14% 14 (14% analyzed Conclusion nonhotspot/novel nonhotspotnovel non hotspot/novel hotspot novel genetics 20 (54 (NGS BRAFNONV VE T599Ifs1 T599Ifs* i.e. (i.e 2 (29 (14 nonhotspot hotspotnovel (5 i.e (2 (1 (

ABSTRACT More information is needed on the potential of using drill cuttings (crushed rocks) from the oil industry in agriculture and forestry. An experiment in forest nursery was carried out to evaluate the influence of substrates formulated from onshore gravel on characteristics of Ipê-branco (Tabebuia roseoalba) seedlings (i.e., growth, quality, and nutrition). We used five gravimetric proportions of gravel from drill cuttings mixed with Pinus-bark - commercial substrate (Mecplant® Florestal 3): control with only commercial substrate and zero gravel (G0), 2.5 % gravel (G2.5), 5 % gravel (G5), 7.5 % gravel (G7.5), and 10 % gravel (G10). In general, high proportion of drill cuttings increases density and decrease current moisture and total porosity of the formulated substrates. The drill cuttings proportions G2.5, G5 and G7.5 significantly contributed to the available water and readily available water in these substrates, with percentage values ranging 23 – 30 % higher than the G10 substrate. Increasing the gravel proportion generally resulted in increased pH levels, P, Na, K and metals (Cu, Fe, Ni, Mn, Cd, Zn, Cr and Pb), except for Ca and Mg nutrients that decreased. Heavy metal contents in all substrates did not exceed the permissible values in legal standards. The G2.5 and G5 substrates increased by 20 % approximately the stem diameter and height of seedlings, and G2.5 proportion also affected the root dry mass and Dickson quality index, with values two times higher than G0 substrate. Multivariate analysis proved suitable as a complementary approach to evaluate the seedling quality. Drilling cuttings addition, in general, increased the accumulation of nutrients and heavy metals of the Ipê-branco seedlings, and G2.5 and G5 substrates provided the greatest accumulation of the nutrients P, Ca, Pb and Zn in the shoot, while G2.5 proportion contributed with higher accumulation of N, Ca, Fe, Cr, Mn and Pb in the root. Adding drill cuttings as a conditioning component of the substrate at 2.5 and 5 % proportions is a viable alternative for using this residue to produce high-quality Tabebuia roseoalba seedling. crushed rocks forestry Ipêbranco Ipê branco i.e., ie i e (i.e. growth nutrition. nutrition . nutrition) Pinusbark Pinus bark Mecplant® Mecplant (Mecplant 3 3) G0, G , (G0) 25 2 2. G25 G2 (G2.5) G5, (G5) 75 7 7. G7.5, G75 G7 (G7.5) 1 G10. (G10) general G7. G1 levels P Na Cu, Cu (Cu Fe Ni Cd Pb, Pb) decreased standards G2. index addition shoot N highquality i.e. (i.e (G0 (G2.5 (G5 (G7.5 (G10 i.e (G (G2. (G7. (G1 (G2 (G7

Phenylphosphonates of zinc (Zn-PPh), calcium (Ca-PPh) and magnesium (Mg-PPh) were synthesized by the hydrothermal method. Samples showed characteristic X-ray diffraction (XRD) patterns of layered compounds with basal spacing in the range of 14-15 Å. According to Fourier transform infrared spectroscopy (FTIR), all compositions showed the presence of characteristic bands attributed to phenyl aromatic rings, phosphonate and lattice metal-oxygen (M-O) vibrations. Different concentrations of phenylphosphonate particles were applied to stabilize water-in-oil Pickering emulsions using vaseline oil in different w/o fractions. The three particle types showed greater affinity for the oil phase (wettability) and the ability to stabilize the emulsions via the Pickering effect. Ca-PPh particles contained a greater emulsified fraction after 20 days of evaluation. This result can be attributed to the particle morphology observed by scanning electron microscopy (SEM). The fibrous morphology of Ca-PPh particles is believed to increase the steric hindrance effect at the surface around the droplets, while the Zn-PPh and Mg-PPh layered particles tended to form clusters. ZnPPh, ZnPPh Zn PPh , (Zn-PPh) CaPPh Ca (Ca-PPh MgPPh Mg (Mg-PPh method Xray X ray XRD (XRD 1415 14 15 14-1 Å FTIR, FTIR (FTIR) rings metaloxygen metal oxygen MO M O (M-O vibrations waterinoil water wo w o fractions wettability (wettability 2 evaluation SEM. SEM . (SEM) droplets clusters (Zn-PPh 141 1 14- (FTIR (SEM

RESUMO O objetivo deste trabalho foi produzir e caracterizar o biocarvão a partir da palha de cana-de-açúcar, bem como investigar possíveis efeitos fitotóxicos às germinações de algodão, milho e soja. Para tanto, o biocarvão foi produzido em forno de duplo tambor e caracterizado por diferentes técnicas. Os ensaios de germinação foram conduzidos in vitro, com doses de biocarvão variando entre 0 e 100 t/ha. O biocarvão foi obtido com rendimento de 40%, pH igual a 9,10, condutividade elétrica de 2,22 dS/m e área superficial específica de 20,3 m2/g. A análise elementar mostrou razões atômicas H/C e O/C de 0,41 e 0,21, respectivamente. A FTIR mostrou a presença de grupos C=C, enquanto a MEV/EDS mostrou estrutura porosa tipo favo de mel e nutrientes, como o Mg, K e Ca. O ensaio de germinação classificou o biocarvão como fitoestimulante para o milho e mostrou ausência de fitotoxicidade para soja. Por outro lado, para o algodão, as doses mais elevadas (50 e 100 t/ha) causaram fitotoxicidade. Os resultados deste trabalho vêm ao encontro das demandas globais por técnicas verdes. O biocarvão feito da palha de cana-de-açúcar é uma tecnologia promissora para melhorar a infertilidade de solos pobres e gerir o sequestro de carbono. canadeaçúcar, canadeaçúcar cana açúcar, açúcar algodão soja tanto vitro 10 tha t ha t/ha 40 40% 910 9 9,10 222 2 22 2,2 dSm dS m 203 20 3 20, m2g mg m2 g m2/g HC H C OC 041 41 0,4 021 21 0,21 respectivamente CC C=C MEVEDS MEV EDS nutrientes Mg Ca lado 50 (5 verdes carbono 1 4 91 9,1 2, 04 0, 02 0,2 5 ( 9,

ABSTRACT The objective of this work was to produce and characterize biochar from sugarcane straw, as well as to investigate possible phytotoxic effects on the germination of cotton, maize, and soybean. To this end, the biochar was produced in a double-drum oven and characterized by different techniques. The germination trials were conducted in vitro, with biochar doses varying between 0 and 100 t/ha. The biochar was obtained with a yield of 40%, pH equal to 9.10, electrical conductivity of 2.22 dS/m and specific surface area of 20.3 m2/g. The elemental analysis showed H/C and O/C atomic ratios of 0.41 and 0.21, respectively. The FTIR showed the presence of C=C groups, while SEM/EDS showed a honeycomb porous structure and nutrients such as Mg, K and Ca. The germination test classified the biochar as a phytonutrient for corn and showed no phytotoxicity for soybeans. On the other hand, for cotton, the highest doses (50 and 100 t/ha) caused phytotoxicity. The results of this work meet the global demands for green techniques. Biochar made from sugarcane straw is a promising technology to improve the infertility of poor soils and manage carbon sequestration. cotton maize soybean end doubledrum double drum techniques vitro 10 tha t ha t/ha 40 40% 910 9 9.10 222 2 22 2.2 dSm dS m 203 20 3 20. m2g mg m2 g m2/g HC H C OC O 041 41 0.4 021 21 0.21 respectively CC groups SEMEDS SEM EDS Mg Ca soybeans hand 50 (5 sequestration 1 4 91 9.1 2. 04 0. 02 0.2 5 ( 9.

RESUMO Esta pesquisa experimental tem como objetivo produzir bioconcretos de casca de arroz (BCCs) com uma matriz de misturas ternárias de cimento, cinza da casca de arroz e cinza volante. A primeira etapa consistiu na avaliação da influência de um aditivo superplastificante e um aditivo polifuncional no comportamento reológico de pastas cimentícias. O objetivo dessa etapa foi testar diferentes teores dos aditivos a fim de desenvolver uma pasta fluida que, após a adição de altas frações volumétricas de casca de arroz (CA), permita obter BCCs leves que possuam trabalhabilidade adequada para adensamento por vibração mecânica. Realizou-se uma trituração e um tratamento químico da CA, no intuito de melhorar sua aderência com a matriz. Os resultados experimentais mostram que o aditivo do tipo PF apresentou melhor compatibilidade com a matriz a um teor de 0,3%. Assim, os BCCs produzidos apresentaram-se coesos e sem segregação, com espalhamento de 210 ± 30 mm. A massa específica seca e a resistência à compressão foram determinadas aos 28 dias de idade. Com os resultados obtidos, os BCCs apresentaram massa específica entre 1000 e 1300 kg/m3 e resistência à compressão superiores a 8,0 MPa, indicando a possibilidade de utilização na produção de painéis leves e autoportantes. (BCCs cimento volante cimentícias CA , (CA) mecânica Realizouse Realizou se 03 0 3 0,3% Assim apresentaramse segregação 21 mm 2 idade obtidos 100 130 kgm3 kgm kg m3 m kg/m 80 8 8, MPa autoportantes (CA 0,3 10 13 0, 1

ABSTRACT This experimental research aims to produce rice husk bio-concretes (RBCs) with a matrix of ternary mixtures of cement, rice husk ash and fly ash. The first step consisted in an evaluation of the influence of a superplasticizer additive and a polyfunctional additive on the rheological behavior of cementitious pastes. The purpose of this step was to test different levels of additives in order to develop a paste that, after adding high volumetric fractions of rice husk, allows obtaining RBCs that have adequate workability for compaction by mechanical vibration. A grinding and chemical treatment process was carried out on the rice husk to improve its adhesion with the matrix. The experimental results show that the PF type additive showed better compatibility with the ternary combination at an optimal content of 0.3%. Thus, the RBCs produced were cohesive, without segregation, with spreading of 210 ± 30 mm. The dry specific mass and compressive strength of the RBCs were determined after 28 days of age. Based on the results obtained, the BCCs presented specific mass between 1000 and 1300 kg/m3 and compressive strength higher than 8.0 MPa, indicating the possibility of use in the production of lightweight and load bearing panels. bioconcretes bio concretes (RBCs cement pastes vibration 03 0 3 0.3% Thus cohesive segregation 21 mm 2 age obtained 100 130 kgm3 kgm kg m3 m kg/m 80 8 8. MPa panels 0.3 10 13 0. 1

Resumo Há uma crescente preocupação com a participação de hospedeiros e reservatórios silvestres na epidemiologia de diversos patógenos, principalmente no contexto das mudanças ambientais e da expansão do conceito "One Health". O objetivo deste estudo foi investigar a presença de hemoplasmas em gambás resgatados da região metropolitana do estado do Rio de Janeiro, Brasil. Amostras de sangue foram coletadas de 15 Didelphis aurita e submetidas à extração de DNA e PCR utilizando-se "primers" para os genes 16S rRNA e 23S rRNA. O exame físico e a análise hematológica também foram realizados. Três dos 15 gambás testaram positivo para Mycoplasma spp. hemotrópico por PCR. Os sinais clínicos eram inespecíficos e associados a lesões traumáticas. Anemia e leucocitose foram detectadas em animais positivos. A análise filogenética indicou que o hemoplasma detectado estava posicionado entre 'Ca. Mycoplasma haemodidelphis' detectado em D. virginiana da América do Norte e hemoplasmas recentemente detectados em D. aurita do estado de Minas Gerais, Brasil. Este estudo indica a existência de infecções por hemoplasmas em D. aurita, da região metropolitana do Rio de Janeiro, e reforça a necessidade de novos inquéritos epidemiológicos, para esclarecer a participação destes na dinâmica de circulação de patógenos transmitidos por carrapatos. One Health. Health . Health" Janeiro Brasil 1 utilizandose utilizando se primers "primers S realizados spp traumáticas positivos Ca. Ca 'Ca haemodidelphis D Gerais epidemiológicos carrapatos

Abstract There is a growing concern about the participation of wild hosts and reservoirs in the epidemiology of several pathogens, particularly within the context of environmental changes and the expansion of the One Health concept. The aim of this study was to investigate the presence of hemoplasmas in opossums rescued from the metropolitan region of Rio de Janeiro state, Brazil. Blood samples were collected from 15 Didelphis aurita and subjected to DNA extraction and PCR using primers for the 16S rRNA and 23S rRNA genes. Physical examination and hematological analysis were also performed. Three out of 15 opossums tested positive for hemotropic Mycoplasma spp. by PCR and showed hematological alterations such as anemia and leukocytosis. Clinical signs were non-specific and associated to traumatic lesions. The phylogenetic analysis indicated that the hemoplasma detected was positioned between 'Ca. Mycoplasma haemodidelphis' detected in D. virginiana from North American and hemoplasmas recently detected in D. aurita from the state of Minas Gerais, Brazil. This study indicates the existence of hemoplasma infections in D. aurita from the metropolitan region of Rio de Janeiro, and reinforce the need for new epidemiological inquiries to clarify the participation of these in the dynamics of circulation of tick-borne pathogens. pathogens concept Brazil 1 S genes performed spp leukocytosis nonspecific non specific lesions Ca. Ca 'Ca haemodidelphis D Gerais tickborne tick borne

Abstract Background Atrial fibrillation (AF) is the most frequent arrhythmia, and its prevalence increases with age. The management of AF in the elderly is challenging, as it is normally associated with comorbidities and frailty. AF catheter ablation (CA) is a safe and superior alternative to antiarrhythmic drugs (AADs) for the maintenance of sinus rhythm. Objectives To evaluate the rate of complications associated with CA for AF across different age groups. Methods A retrospective analysis of 219 patients who underwent CA for AF between 2016 and 2020 were divided into 3 age groups: less than 60 years, 60 to 70 years, and > 70 years. All the included patients underwent radiofrequency ablation using an electroanatomic mapping system. Categorical variables were evaluated with chi-square and Fisher’s test, and continuous variables were evaluated by Kruskal-Wallis and post-hoc Tamhane’s T2. P values less than 0.05 were considered significant. Results We found an overall total complication rate of 4.6%. The total complication rate was 3.3% in patients < 60 years of age, 5.7% in patients between 60 and 70 years, and 5.2% in patients > 70 years (p = 0.742). No deaths occurred. Conclusion There was no significant difference in the AF CA-related complications when comparing the patients by age group. (AF arrhythmia challenging frailty (CA AADs (AADs rhythm groups 21 201 202 6 7 system chisquare chi square Fishers Fisher s test KruskalWallis Kruskal Wallis posthoc post hoc Tamhanes Tamhane T2 T 005 0 05 0.0 46 4 4.6% 33 3.3 57 5 5.7 52 2 5.2 p 0.742. 0742 0.742 . 742 0.742) occurred CArelated related group 20 00 0. 4.6 3. 5. 074 0.74 74 4. 07 0.7

Abstract Objective Investigate the auditory function of the elderly using the middle latency potentials. Methodology Group 1 (G1): 20 healthy individuals of both genders, older than 60 years, without hearing loss. Group 2 (G2): 20 healthy individuals of both sexes, older than 60 years, with hearing loss in frequencies from 4 to 8 kHz. Potential recording was performed with unilateral and bilateral stimulation and the Binaural Interaction Component was calculated. Results Na latency in C3A1 was greater in the stimulation of the right ear in G2 and the amplitude of Na-Pa was greater in the stimulation of the right ear and recording in C3A1 in G1. The latency of the Pa component was higher in the stimulation of the right ear recorded in C4A2. The Pb component in G2 by bilateral stimulation and recorded in C4A2 had higher latency. The first and second negative and positive peaks presented greater amplitude in G1. In C3A1, the 1st negative peak was more negative in G1 and the 2nd positive peak showed greater amplitude in C4A2 in both groups. Conclusion The transmission of auditory information to the primary auditory cortex is impaired with aging, especially in unilateral stimulation, reinforced by losses in elderly people with peripheral hearing loss, such as in the binaural interaction at the cortical and subcortical levels. Thus, the AMLR has shown to be a sensitive examination to investigate neuroauditory disorders in the elderly, especially related to high-frequency hearing loss and primary auditory cortex dysfunctions caused by the aging process. potentials G (G1) genders 6 years (G2) sexes kHz calculated CA C A C3A NaPa C4A st nd groups levels Thus highfrequency high frequency process (G1 (G2 (G

Electrospinning was used to produce poly(vinyl alcohol) (PVA)-based nanofibers containing nerolidol (NER) as the antimicrobial agent. The scanning electron microscopy results indicated the formation of homogeneous fibers in the absence of NER. However, its incorporation into the fiber prevented the complete solvent evaporation, ultimately leading to the coalescence of the fibers. It became more evident as the amount of NER increased from 0 to 50% m/v. The incorporation of NER also decreased the crystallinity of the fibers from 10 to ca. 6.5%. However, this decrease was not proportional to the concentration of NER. The contact angle results confirmed the presence of NER molecules on the surface of the fibers especially due to the increase of the contact angle from 0 (PVA/NER0) to 27.8º (PVA/NER50). The fibrous mats exhibited good antimicrobial activity, being even more efficient than the control in some cases, suggesting their potential for wound-dressing purposes. polyvinyl poly vinyl alcohol PVAbased PVA based (NER agent However evaporation 50 mv m v m/v 1 ca 65 6 5 6.5% PVA/NER0 PVANER0 PVANER NER0 (PVA/NER0 278º º 27 8º PVA/NER50. PVANER50 PVA/NER50 . NER50 (PVA/NER50) activity cases wounddressing wound dressing purposes 6.5 PVA/NER (PVA/NER 2 PVANER5 PVA/NER5 NER5 (PVA/NER50 6. (PVA/NER5

Abstract The identification of putative prognostic factors in canine mammary neoplasms (CMNs) has been focused on tissue-specific biomarkers, but the serum biomarkers, including cancer antigen 15-3 (CA 15-3), c-reactive protein (CRP), and lactate dehydrogenase (LDH) have been demonstrated to display clinical application in cases of CMNs. The aim of the study was to evaluate the levels of these serum biomarkers and their association with well-established prognostic factors in CMNs. Samples from 15 female canines with CMNs and 15 clinically healthy ones were collected. The results were evaluated using the Tukey’s, Pearson, or Spearman tests. The cut-off point, sensitivity, specificity, and area under curve (AUC) were evaluated using the receiver operating characteristic (ROC) curve analysis in a logistic regression model (P<0.05). The levels of CA 15-3, CRP and LDH were significantly higher in the serum of female dogs with CMNs compared to the healthy ones. Moreover, these factors were positively correlated with ulceration, tumor size, histopathological grade, metastatic lymph node, and clinical staging. Female dogs with CMNs were found to exhibit highest serum levels of CA 15-3, CRP, and LDH. Therefore, they can be applied to improve the efficacy of the diagnosis and prognostic evaluation in casas of CMNs. (CMNs tissuespecific tissue specific 153 3 15- 153, , 15-3) creactive c reactive (CRP) (LDH wellestablished well established 1 collected Tukeys, Tukeys Tukey s, s Tukey’s Pearson tests cutoff cut off point sensitivity specificity AUC (AUC ROC (ROC P<0.05. P005 P P<0.05 . 0 05 (P<0.05) 3, Moreover ulceration size grade node staging Therefore (CRP P00 P<0.0 (P<0.05 P0 P<0. (P<0.0 P<0 (P<0. P< (P<0 (P< (P