ABSTRACT The research evaluated the isolation, identification, and prospection of species-specific beneficial bacteria in fish farming of ornamental fish Betta splendens. For this, the microbiological material was obtained from the intestinal tract of healthy specimens, with bacterial growth in selective culture medium Man Rogosa Sharped (MRS). Sixteen strains were isolated based on the response of in vitro tests of catalase, Gram, nilin blue, hemolytic activity and antibiogram to pathogens Aeromonas hydrophila, Pseudomonas aeroginosa, Enterococcus durans and Escherichia coli. Of the isolated strains C1BS and C5BS, they showed the best responses, which were later identified by the Maldi-TOF method as Lactobacillus plantarum and Enterococcus faecium. Due to the performance of lactic acid strains in in vitro tests and the bibliographic record of their performance as probiotics, the species have great potential for species-specific use in the ornamental production of Betta splendens. isolation identification speciesspecific specific splendens this specimens MRS. MRS . (MRS) catalase Gram blue hydrophila aeroginosa coli CBS C BS C5BS responses MaldiTOF Maldi TOF faecium probiotics (MRS

RESUMO A pesquisa avaliou o isolamento, a identificação e a prospecção de bactérias benéficas espécie-específico na piscicultura do peixe ornamental Betta splendens. Para isso, o material microbiológico foi obtido do trato intestinal de espécimes sadios, com crescimento bacteriano em meio de cultura seletivo Man Rogosa Sharped (MRS). Dezesseis cepas foram isoladas com base na resposta dos testes in vitro de catalase, Gram, azul de anilina, atividade hemolítica e antibiograma aos patógenos Aeromonas hydrophila, Pseudomonas aeroginosa, Enterococcus durans e Escherichia coli. Das cepas isoladas, C1BS e C5BS foram as que apresentaram melhores respostas, as quais posteriormente foram identificadas, pelo método Maldi-TOF, como Lactobacillus plantarum e Enterococcus faecium. Devido ao registro bibliográfico e ao desempenho das cepas ácido lácticas nos testes in vitro, essas apresentam um grande potencial para uso probiótico espécie-específico na produção ornamental de Betta splendens. isolamento espécieespecífico espécie específico splendens isso sadios MRS. MRS . (MRS) catalase Gram anilina hydrophila aeroginosa coli CBS C BS respostas identificadas MaldiTOF, MaldiTOF Maldi TOF, TOF Maldi-TOF faecium (MRS

G‐quadruplexes (G4) are structures formed at the ends of telomeres rich in guanines and stabilized by molecules that bind to specific sites. TMPyP4 and thymoquinone (TQ) are small molecules that bind to G4 and have drawn attention because of their role as telomerase inhibitors. The aim of this study was to evaluate the effects of telomerase inhibitors on cellular proliferation, senescence, and death. Two cell lines, LC‐HK2 (non-small cell lung cancer - NSCLC) and RPE‐1 (hTERT-immortalized), were treated with TMPyP4 (5 μM) and TQ (10 μM). Both inhibitors decreased telomerase activity. TMPyP4 increased the percentage of cells with membrane damage associated with cell death and decreased the frequency of cells in the S‐phase. TMPyP4 reduced cell adhesion ability and modified the pattern of focal adhesion. TQ acted in a concentration-dependent manner, increasing the frequency of senescent cells and inducing cell cycle arrest in G1 phase. Thus, the present results showed that TMPyP4 and TQ, although acting as telomerase inhibitors, had a broader effect on other signaling pathways and processes in cells, differing from each other. However, they act both on malignant and immortalized cells, and further studies are needed before their anti-cancer potential can be considered. Gquadruplexes G quadruplexes (G4 sites TMPyP (TQ proliferation senescence lines LCHK2 LCHK LC HK2 HK LC‐HK nonsmall non NSCLC RPE1 RPE 1 RPE‐ hTERTimmortalized, hTERTimmortalized hTERT , (hTERT-immortalized) 5 ( μM 10 (1 μM. . activity Sphase S phase S‐phase concentrationdependent concentration dependent manner Thus However anticancer anti considered (G (hTERT-immortalized

We studied the effect of pulsed ultrasound therapy (UST) and antibothropic polyvalent antivenom (PAV) on the regeneration of mouse extensor digitorum longus muscle following damage by Bothrops jararacussu venom. Animals (Swiss male and female mice weighing 25.0 ± 5.0 g; 5 animals per group) received a perimuscular injection of venom (1 mg/kg) and treatment with UST was started 1 h later (1 min/day, 3 MHz, 0.3 W/cm², pulsed mode). Three and 28 days after injection, muscles were dissected and processed for light microscopy. The venom caused complete degeneration of muscle fibers. UST alone and combined with PAV (1.0 mL/kg) partially protected these fibers, whereas muscles receiving no treatment showed disorganized fascicules and fibers with reduced diameter. Treatment with UST and PAV decreased the effects of the venom on creatine kinase content and motor activity (approximately 75 and 48%, respectively). Sonication of the venom solution immediately before application decreased the in vivo and ex vivo myotoxic activities (approximately 60 and 50%, respectively). The present data show that UST counteracts some effects of B. jararacussu venom, causing structural and functional improvement of the regenerated muscle after venom injury.

Verificaram-se os efeitos da associação de furosemida e fenilbutazona sobre variáveis hidroeletrolíticas de cavalos antes e após a corrida. Dezenove equinos foram distribuídos em três grupos, de acordo com os protocolos de tratamento. O primeiro grupo, de cinco animais, não recebeu medicação (grupo-controle); o segundo grupo, de sete animais, foi tratado com furosemida, na dose de 1mg/kg, por via intramuscular, até quatro horas antes do páreo; o terceiro, de sete animais, recebeu furosemida, por via intramuscular, e fenilbutazona, por via intravenosa, nas doses de 1,0 e 4,4mg/kg, respectivamente, até quatro horas antes da corrida. Amostras de sangue foram colhidas antes, imediatamente após e duas horas após o páreo, para avaliação da osmolalidade plasmática e das concentrações plasmáticas de sódio, potássio e cloreto. A utilização de furosemida e da associação furosemida e fenilbutazona até 4h antes dos páreos nas dosagens descritas alterou (P<0,05) a osmolalidade plasmática dos equinos, mas não alterou (P>0,05) as concentrações de sódio, potássio e cloreto. Os páreos alteraram de forma fisiológica a osmolalidade plasmática e a concentração sanguínea de K+ devido ao exercício de alta intensidade.

The objective of this study was to verify the effects of furosemide and phenylbutazone association on fluid and electrolyte balance characteristics of horses before and after a race. Nineteen horses were divided into three groups according to treatment protocols. The first group (five animals - control) was not medicated. A second group (seven animals) was treated with furosemide (1mg/kg, intramuscular up to four hours before the race). A third group (seven animals) received furosemide (1mg/kg) and phenylbutazone (4.4mg/kg), both intramuscular, up to four hours before race. Blood samples were collected before, immediately after and two hours after a race to evaluate the plasma osmolality and sodium, potassium and chloride concentrations. The use of furosemide and furosemide plus phenylbutazone up to four hours before the race altered (P<0.05) the plasma osmolality but did not change (P>0.05) the sodium, potassium and chloride concentrations. It was not possible to determine an antagonist effect of phenylbutazone on furosemide, based on fluid and electrolyte balance. Due to the high intensity exercise, the increase in plasma osmolality and potassium concentration was attributed to the race effect.