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INTELLIGENT IDENTIFICATION OF RICE GROWTH PERIOD (GP)BASED ON RAMAN SPECTROSCOPY AND IMPROVED CNN IN HEILONGJIANG PROVINCE OF CHINA
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Liu, Rui

; Tan, Feng

; Ma, Bo

Engenharia Agrícola

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Dez 2023, Volume 43 Nº 6 elocation e20230127

Resumo: En | Texto: En | PDF: En

ABSTRACT The fertile land in Heilongjiang Province of China is suitable for rice cultivation, but this area is susceptible to low temperature and chilling injury, which is prevented by planting rice varieties suitable for GP that is an important measure. However, selection based on rice traits is vulnerable to environmental influences and takes a long time, and selection based on molecular markers may result in progeny recombination and lack of reliability. Therefore, an efficient accurate and intelligent identification method for rice growth period is urgently needed. In this study, machine learning and deep learning methods in Python were used to analyze the Raman spectra of 6 rice varieties in three accumulated temperature region of Heilongjiang Province. 1) In machine learning, Principal Component Analysis (PCA) was adopted for feature extraction, in combination with Support Vector Machine (SVM) classification models suitable for nonlinear data sets for identification, the identification rate was 93.33% and the type of this experimental data set was determined to be discrete. 2) In deep learning, Continuous Wavelet Transform (CWT) methods was adopted for data preprocessing, combined with the Convolutional Neural Networks (CNN) model with its own feature extraction, with the highest accuracy of 94.82%, which was higher than the PCA+SVM identification model. 3) Based on the method mentioned in 2), in order to improve the feature extraction ability of the model as a whole, Convolutional Block Attention Module (CBAM) was used to improve the CNN identification model for the first time for one-dimensional data sets, and the highest identification rate was 98.28%, which was better than the PCA+SVM identification model. 3) In the verification test, Raman spectral information of 4 rice varieties was brought into the constructed CWT+CNN-CBAM identification model for identification, and the identification results were as high as 94.79%. The experimental results showed that the CWT visualization data processing method based on Raman technology combined with the CNN identification model of CBAM with improved feature extraction ability in deep learning achieved the best identification results, which could provide an efficient, accurate and intelligent method for the identification of different growth period of rice varieties in Heilongjiang Province.

https://doi.org/10.1590/1809-4430-eng.agric.v43n6e20230127/2023

Corn straw return effectively improves the stability and increases the carbon and nitrogen contents of waterstable aggregates in northeastern China black soil
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Xie, Shuai

; Dou, Sen

; Fu, Jing

; Ma, Rui

Bragantia

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2023, Volume 82 elocation e20220218

Resumo: En | Texto: En | PDF: En

ABSTRACT In view of the current situation of black soil degradation, straw return as an important conservation tillage measure has been extensively promoted. Based on 15N tracing technology, this paper carried out experiments of different straw returning modes, including CK (conventional fertilizing tillage with straw-free returning), straw mulching (i.e., M), straw mixed with topsoil (i.e., T), and straw deep incorporation (i.e., D), to explore the influence of straw returning on the distribution and stability, and the carbon and nitrogen content of water-stable aggregates in black soil, and to analyze the distribution and stability of aggregates on the carbon and nitrogen content of aggregates. The results showed that the macroaggregate content, mean weight diameter (MWD) and geometric mean diameter (GMD) of the returned soil layers were 16.53-84.65%, 16.73-128.73% and 23.47-97.14% higher than those in CK, respectively. The contents of organic carbon, total nitrogen and 15N accumulation of aggregates in the straw-returning soil layer were 6.38-23.55%, 8.65-31.19% and 13.52-150.19% higher than those in CK, respectively. Pearson correlation analysis and redundancy analysis showed that the content of macroaggregates and stability of aggregates were positively correlated with the carbon and nitrogen contents. In conclusion, straw return significantly improved soil structure characteristics and carbon and nitrogen content. The results of this study provided a theoretical basis and technical guidance for farmland soil improvement in black soil areas, and selected an appropriate straw returning mode according to local soil conditions to maximize the effect of straw returning.

https://doi.org/10.1590/1678-4499.20220218

Abordagem de Bioinformática e Biologia de Sistemas para Identificar a Ligação Patogenética entre Insuficiência Cardíaca e Sarcopenia
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Xu, Rui

; Ma, Ling-ling

; Cui, Shuai

; Chen, Ling

; Xu, Hong

Arquivos Brasileiros de Cardiologia

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2023, Volume 120 Nº 10 elocation e20220874

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Background Despite increasing evidence that patients with heart failure (HF) are susceptible to sarcopenia, the reason for the association is not well understood. Objective The purpose of this study is to explore further the molecular mechanism of the occurrence of this complication. Methods Gene expression datasets for HF (GSE57345) and Sarcopenia (GSE1428) were obtained from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were identified using ‘edgeR’ and “limma” packages of R, and their functions were analyzed using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). Protein-protein interaction (PPI) networks were constructed and visualized using Search Tool for the Retrieval of Interacting Genes (STRING) and Cytoscape. Hub genes were selected using the plugin cytoHubba and validation with GSE76701 for HF and GSE136344 for Sarcopenia. The related pathways and molecular mechanisms of the hub genes were performed by Gene set enrichment analysis (GSEA). The statistical analyses were performed using R software. P < 0.05 was considered statistically significant. Results A total of 114 common DEGs were found. Pathways related to growth factor, Insulin secretion and cGMP-PKG were enriched in both HF and Sarcopenia. CYP27A1, KCNJ8, PIK3R5, TIMP2, CXCL12, KIT, and VCAM1 were found to be significant hub genes after validation, with GSEA emphasizing the importance of the hub genes in the regulation of the inflammatory response. Conclusion Our study reveals that HF and Sarcopenia share common pathways and pathogenic mechanisms. These findings may suggest new directions for future research into the underlying pathogenesis.

Resumo Fundamento Apesar das evidências crescentes de que pacientes com insuficiência cardíaca (IC) são suscetíveis à sarcopenia, o motivo da associação não é bem compreendido. Objetivo O objetivo deste estudo é explorar ainda mais o mecanismo molecular de ocorrência desta complicação. Métodos Conjuntos de dados de expressão gênica para HF (GSE57345) e Sarcopenia (GSE1428) foram obtidos do banco de dados Gene Expression Omnibus (GEO). Genes diferencialmente expressos (DEGs) foram identificados usando pacotes ‘edgeR’ e “limma” de R, e suas funções foram analisadas usando Gene Ontology (GO) e a Enciclopédia de Genes e Genomas de Kyoto (KEGG). Redes de interação proteína-proteína (PPI) foram construídas e visualizadas usando Search Tool for the Retrieval of Interacting Genes (STRING) e Cytoscape. Os genes hub foram selecionados usando o plugin cytoHubba e validados com GSE76701 para IC e GSE136344 para Sarcopenia. As vias relacionadas e os mecanismos moleculares dos genes hub foram realizados pela análise de enriquecimento de genes (GSEA). As análises estatísticas foram realizadas no software R. P < 0,05 foi considerado estatisticamente significativo. Resultados Foram encontrados 114 DEGs comuns. As vias relacionadas ao fator de crescimento, secreção de insulina e cGMP-PKG estavam enriquecidas tanto na IC quanto na sarcopenia. Descobriu-se que CYP27A1, KCNJ8, PIK3R5, TIMP2, CXCL12, KIT e VCAM1 são genes hub significativos após validação com GSEA enfatizando a importância dos genes hub na regulação da resposta inflamatória. Conclusão Nosso estudo revela que a IC e a Sarcopenia compartilham vias e mecanismos patogênicos comuns. Estes achados podem sugerir novas direções para pesquisas futuras sobre a patogênese subjacente.

https://doi.org/10.36660/abc.20220874

Serum lymphocytes and cytokines: diagnostic value and influence on the immune status in patients with pulmonary tuberculosis
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Ma, Zhiqiang

; Li, Shenghao

; Liu, Yuan

; Li, Caixin

; Wang, Xiaoyan

; Tang, Xingrui

; Dong, Rui

; Zheng, Shitai

; Wang, Lin

Jornal Brasileiro de Pneumologia

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2023, Volume 49 Nº 5 elocation e20230154

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT Objective: To determine the absolute number of serum T lymphocytes and cytokine levels and the characteristics of patients with active pulmonary tuberculosis and to assess their effect on the immune status of these patients and their diagnostic and predictive value for tuberculosis. Methods: We included 1,069 patients with active tuberculosis, 51 patients with latent tuberculosis infection, and 600 health individuals. Absolute serum T-lymphocyte counts and cytokine levels were quantified. Results: T lymphocytes were significantly reduced in patients with active tuberculosis when compared with healthy individuals. The immune function of patients gradually decreased with age and was stronger in female patients than in males. Th1 cells expressed higher levels of cytokines than did Th2 cells. Logistic regression analysis showed that reduced CD3+ T, CD8+ T, and NK cell counts, as well as reduced IL-4 and IFN-g expression, were independent influencing factors for active tuberculosis. ROC analysis showed that the sensitivity and specificity of absolute CD3+ T and CD8+ T lymphocyte counts and combined factors were significantly higher than were those of IL-4 and IFN-g for diagnosing active tuberculosis. Conclusions: Serum T-lymphocyte counts and cytokine levels can assess the immune status of tuberculosis patients; they are also useful biomarkers for predicting and diagnosing tuberculosis.

RESUMO Objetivo: Determinar o número absoluto de linfócitos T séricos e os níveis de citocinas séricas, bem como as características, de pacientes com tuberculose pulmonar ativa e avaliar o efeito desses no estado imunológico desses pacientes e seu valor diagnóstico e preditivo para tuberculose. Métodos: Foram incluídos 1.069 pacientes com tuberculose ativa, 51 pacientes com tuberculose latente e 600 indivíduos saudáveis. Foram realizadas a contagem absoluta de linfócitos T séricos e a quantificação de citocinas séricas. Resultados: Os linfócitos T estavam significativamente reduzidos nos pacientes com tuberculose ativa em comparação com os indivíduos saudáveis. A função imunológica dos pacientes diminuiu gradativamente com a idade e mostrou-se mais forte nas mulheres do que nos homens. As células Th1 expressaram maiores níveis de citocinas do que as células Th2. A análise de regressão logística mostrou que contagens reduzidas de células T CD3+, T CD8+ e NK e expressão reduzida de IL-4 e IFN-g foram fatores de influência independentes para tuberculose ativa. A análise ROC mostrou que a sensibilidade e especificidade dos valores absolutos de linfócitos T CD3+ e T CD8+ e de fatores combinados foram significativamente maiores do que as da IL-4 e do IFN-g para o diagnóstico da tuberculose ativa. Conclusões: A contagem de linfócitos T séricos e os níveis de citocinas séricas podem avaliar o estado imunológico de pacientes com tuberculose; também são biomarcadores úteis na predição e diagnóstico da tuberculose.

https://doi.org/10.36416/1806-3756/e20230154

Circulating miRNA-1-3p as Biomarker of Accelerated Sarcopenia in Patients Diagnosed with Chronic Heart Failure
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Xu, Rui

; Cui, Shuai

; Chen, Ling

; Chen, Xin-Chun

; Ma, Ling-Ling

; Yang, Hong-Ni

; Wen, Fang-Mei

Revista de investigación clínica

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Out 2022, Volume 74 Nº 5 Páginas 276 - 283

Resumo: En | Texto: En | PDF: En

ABSTRACT Background: While sarcopenia is an important clinical finding in individuals diagnosed with chronic heart failure (CHF), efforts to identify a reliable biomarker capable of predicting the overall muscular and functional decline in CHF patients have been unsuccessful to date. Objectives: The objectives of this study were to study the diagnostic utility of MicroRNA (miRNA)-1-3p as a predictor of sarcopenia status in individuals diagnosed with CHF. Methods: In total, 80 individuals with heart failure exhibiting a left ventricular ejection fraction < 50% were enrolled in this study. All patients were analyzed to assess miR-1-3p expression levels, with body composition being evaluated through dual-energy X-ray absorptiometry and sarcopenia being defined based on the sum of appendicular lean muscle mass (ALM) divided by height in meters squared and handgrip strength (HGS). In addition, the activation of the Akt/mTOR signaling pathway was evaluated in these individuals. Results: In total, 40 of the enrolled patients (50%) exhibited sarcopenia. Sarcopenic patients presented with increased miR-1-3p expression levels as compared to non-sarcopenic individuals (1.69 ± 0.132 vs. 1.22 ± 0.106; p < 0.05). With respect to sarcopenic indices, appendicular skeletal mass index was most strongly correlated with miR-1-3p expression, which was also strongly correlated with HGS. High levels of Akt/mTOR signaling pathway components were expressed in sarcopenic individuals, highlighting a significant relationship between miR-1-3p activity and signaling through this pathway. Moreover, miR-1-3p was identified as a specific marker for sarcopenia in individuals with CHF. Conclusion: These results suggest that circulating miR-1-3p levels are related to Akt/mTOR pathway activation and can offer valuable insight into the overall physical capacity and muscular integrity of CHF patients as a predictor of sarcopenia.

https://doi.org/10.24875/ric.22000151

Effect of ozone treatment on physicochemical parameters and ethylene biosynthesis inhibition in Guichang Kiwifruit
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CAO, Sen

; MENG, Lingshuai

; MA, Chao

; BA, Liangjie

; LEI, Jiqing

; JI, Ning

; WANG, Rui

Food Science and Technology

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2022, Volume 42 elocation e64820

Resumo: En | Texto: En | PDF: En

Abstract To determine whether ozone can regulate fruit ripening and delay fruit aging, Kiwifruit (Actinidia deliciosa 'Guichang') was fumigated with different ozone concentrations (100 μL/L, 200 μL/L, 300 μL/L) for 3 h, stored at 1 °C and 85 ± 5% RH for three months, and then matured at 20 °C and 85% RH for 12 days. Compared with controls, the optimal concentration of ozone delayed kiwifruit ripening during cold storage by delaying the respiratory burst that precedes fruit decay. Consequently, fruit firmness, nutrient content, and enzyme activity remained stable; the expression of AdACS1 and AdACO1 as well as the activity of ACC synthetase (ACS) and ACC oxidase (ACO) was downregulated, whereby ACC and MACC were accumulated. In addition, ozone-treated fruit showed significantly reduced infection with grey mold. The effect of ozone on kiwifruit at 200 μL/L was the best suited for delaying fruit senescence, maintaining high quality during storage and fruit ripening compared with other fruits. Overall, our study indicated that ozone may play a major residual role in the physiological process of fruit ripening; furthermore, ozone can affect ethylene biosynthesis and fruit changes associated with endogenous ethylene production.

https://doi.org/10.1590/fst.64820

Effects of melatonin treatment on the physiological quality and cell wall metabolites in kiwifruit
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CAO, Sen

; QU, Guangfan

; MA, Chao

; BA, Liangjie

; JI, Ning

; MENG, Lingshuai

; LEI, Jiqing

; WANG, Rui

Food Science and Technology

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2022, Volume 42 elocation e85421

Resumo: En | Texto: En | PDF: En

Abstract We investigated the effects of melatonin (MT) treatment on the physiological quality and cell wall metabolism of kiwifruit during a 12-day shelf life period (20 ± 1 °C, 90 ± 5% RH), after 90 days of cold storage (0 ± 0.5 °C, 90 ± 5% RH). Our results showed that MT treatment delayed kiwifruit softening, as evidence by the enhanced endurance of a healthy appearance, quality, color difference L* value, and firmness of the fruit flesh. MT treatment reduced fruit decay rate, respiration rate, and malondialdehyde content; furthermore, MT-treated kiwifruit maintained high levels of total soluble solids, titratable acid, and ascorbic acid. Additionally, MT inhibited the decrease in fruit pectin, cellulose, and hemicellulose contents; it delayed the increase in water-soluble pectin content and kept pectin methyl esterase, polygalacturonase, cellulase, and β-galactosidase activities low. These results indicated that melatonin may have reduced the speed of softening of kiwifruit and preserved fruit quality by inhibiting enzymatic degradation of the cell wall, and by delaying pro-pectin, cellulose, and hemicellulose degradation, as well as the increase of water-soluble pectin content. These findings indicate that MT treatment may be an effective measure to delay kiwifruit postharvest cell-wall metabolism and preserve fruit quality.

https://doi.org/10.1590/fst.85421

Effects of 1-methylcyclopene and controlled-atmosphere treatment on the quality and antioxidant capacity of blueberries during storage
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BA, Liangjie

; LUO, Donglan

; JI, Ning

; CAO, Sen

; MA, Chao

; ZHANG, Peng

; WANG, Rui

Food Science and Technology

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2022, Volume 42 elocation e60220

Resumo: En | Texto: En | PDF: En

Abstract The effects of 1-methylcyclopropene (1-MCP) treatment and controlled-atmosphere (CA) storage and their combination on quality and antioxidant capacity of postharvest blueberries were evaluated here. To investigate the most appropriate approach for maintaining fruit quality, blueberries were subjected to four different treatments, a control (CK), 1-MCP, 3% O2 + 10% CO2 (CA), and 1-MCP combined with 3% O2+10% CO2 (1-MCP + CA), then stored for 80 days at 0 °C. It was found that the applications of 1-MCP, CA and 1-MCP + CA were effective at delaying the increase in weight loss and inhibiting the decrease in firmness, total soluble solids (TSS) contents and titratable acidity (TA) contents. In addition, 1-MCP, CA and 1-MCP + CA treatment could significantly suppress MDA accumulation and relative electrolytic leakage promotion. Moreover, the production of reactive oxygen species (ROS), such as O2- and H2O2, was reduced by all the treatment, which was associated with increased activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX). Taken together, our findings indicate 1-MCP, CA and 1-MCP + CA have potential applications in maintaining the quality and improving the antioxidant capacity of harvested blueberries, and 1-MCP + CA is the most effective treatment.

https://doi.org/10.1590/fst.60220

Exogenous melatonin treatment in the postharvest storage of pitaya fruits delays senescence and regulates reactive oxygen species metabolism
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BA, Liangjie

; CAO, Sen

; JI, Ning

; MA, Chao

; WANG, Rui

; LUO, Donglan

Food Science and Technology

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2022, Volume 42 elocation e15221

Resumo: En | Texto: En | PDF: En

Abstract Pitaya fruits have high senescence rates throughout their postharvest storage period. Additionally, studies have confirmed that melatonin plays a regulatory role in plant senescence. However, the involvement of melatonin in the postharvest senescence of fruits remains unclear. In this study, two cultivars of pitaya fruit, ‘Zihonglong’ and ‘Jinghonglong’, were treated with melatonin and then evaluated for characteristics of senescence while in storage for 10 days. The results showed that melatonin treatment delayed fruit senescence in both pitaya cultivars, as indicated by the inhibition of weight loss, decay incidence, relative membrane permeability, and malondialdehyde (MDA) content, as well as the maintenance of the total soluble solids and ascorbic acid contents and the reduced respiration intensity. In addition, melatonin treatment reduced the O2·- production rates, H2O2 contents, and lipoxygenase activities but enhanced the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) in both pitaya cultivars. These results indicate that melatonin may contribute to delaying senescence in pitaya fruits. This study shows the potential of the use of melatonin in the postharvest storage of pitaya fruits, as well as other horticultural fruits.

https://doi.org/10.1590/fst.15221

10.

Effects of foliar iron spraying on Cabernet Sauvignon phenolic acids and proanthocyanidins
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WANG, Jingyao

; GU, Chaofeng

; MA, Tinghui

; WANG, Rui

Food Science and Technology

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2022, Volume 42 elocation e44622

Resumo: En | Texto: En | PDF: En

Abstract In order to explore the effect of different irons on the fruit quality of wine grapes, as well as the phenolic acids and proanthocyanidins of the peel, “Cabernet Sauvignon” was taken as the research object. Ferrous sulfate, EDTA-Fe, ferric citrate, ferric gluconate and ferric sugar alcohol were sprayed between the coloring periods, and the foliar sprayed water was used as the control. The results showed that foliar spraying of iron would increase the fruit sugar-acid ratio and 100-grain weight; iron spraying would up-regulate the content of phenolic acid monomers, among which EDTA-Fe and ferric citrate had significant up-regulating effects, but all iron treatments had a significant effect on the increase. The content of caffeic acid and chlorogenic acid was down-regulated; the content of proanthocyanidin monomer was up-regulated by iron spraying, and the content of proanthocyanidin monomer was significantly up-regulated by ferric citrate treatment. Foliar spraying of ferric citrate not only balanced the sugar-acid ratio of the fruit, but also increased the content of phenolic acid and proanthocyanidin in the grape peel, which further improved the quality of the grapes and helped to increase the flavor of the wine.

https://doi.org/10.1590/fst.44622

11.

Effects of melatonin on blueberry fruit quality and cell wall metabolism during low temperature storage
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QU, Guangfan

; BA, Liangjie

; WANG, Rui

; LI, Jiangkuo

; MA, Chao

; JI, Ning

; CAO, Sen

Food Science and Technology

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2022, Volume 42 elocation e40822

Resumo: En | Texto: En | PDF: En

Abstract Melatonin is known to influence various physiological processes, including plant ripening; however, the effect of melatonin on postharvest blueberry fruit softening is unknown. Herein, the effects of melatonin at different concentrations (100, 300, and 500 μmol L-1) on the quality parameters, cell wall substances, cell-wall-degrading enzymes, and cell wall ultrastructure of blueberry fruits were investigated during low-temperature storage. Melatonin treatment was found to effectively delay the decline in fruit firmness, slow the loss of soluble solids and titratable acids, and decrease the respiration rate and decay rate of blueberry fruits. The blueberry fruits treated with 300 μmol L-1 melatonin exhibited the best quality. Melatonin treatment promoted anthocyanin accumulation, delayed ascorbic acid degradation, maintained a low relative membrane permeability in pulp, inhibited increases in lipoxygenase (LOX) activity, and reduced membrane lipid peroxidation. Moreover, melatonin controlled the formation of soluble pectin by inhibiting the activities of pectin methylesterase (PME), polygalacturonase (PG), cellulase (Cx), and β-glucosidase (β-Glu) while maintaining high contents of protopectin and cellulose. These results indicate that melatonin can effectively improve blueberry fruit quality and antioxidant capacity as well as and delay fruit softening by inhibiting the degradation of cell wall substances.

https://doi.org/10.1590/fst.40822

12.

Neuropathologic damage induced by radiofrequency ablation at different temperatures
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Dong, Yu

; Chen, Ying

; Yao, Baoguo

; Song, Peng

; Xu, Ruiting

; Li, Rui

; Liu, Ping

; Zhang, Yu

; Mu, Li

; Tong, Xin

; Ma, Linwei

; Yu, Jianjun

; Su, Li

Clinics

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2022, Volume 77 elocation 100033

Resumo: En | Texto: En | PDF: En

Abstract Objective To explore the molecular mechanism of neuropathologic damage induced by radiofrequency ablation at different temperatures. Methods This is basic research, and 36 SD rats were used to construct the neuropathological injury model. The rats were subjected to radiofrequency stimulation at different temperatures and were divided into 6 groups according to the temperature injury: 42°, 47°, 52°, 57°, 62°, and 67°C groups. Conduction time, conduction distance, and nerve conduction velocity were recorded after temperature injury. HE-staining was used to observe the histopathological morphology of the sciatic nerve. The expression of SCN9A, SCN3B, and NFASC protein in sciatic nerve tissue were detected by western blot. Results With the increase in temperature, nerve conduction velocity gradually decreased, and neurons were damaged when the temperature was 67°C. HE-staining showed that the degrees of degeneration of neurons in rats at 47°, 52°, 57°, 62°, and 67°C were gradually increased. The expression of SCN9A, SCN3B protein in 57°, 62°, 67°C groups were much higher than that of NC, 42°, 47°, 52°C groups. However, the expression of NFASC protein in 57°, 62°, 67°C groups was much lower than that of the NC, 42°, 47°, 52°C groups. Conclusion There was a positive correlation between temperature caused by the radiofrequency stimulation to neuropathological damage. The mechanism is closely related to the expression of SCN9A, SCN3B, and NFASC protein in nerve tissue caused by heat transfer injury.

https://doi.org/10.1016/j.clinsp.2022.100033

13.

CHANGES IN THE FLEXOR AND EXTENSOR MUSCLES AT DIFFERENT SPEEDS
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Ma, Rui

; Li, Wenyan

Revista Brasileira de Medicina do Esporte

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Jul 2021, Volume 27 Nº 7 Páginas 686 - 688

Resumo: Pt En Es | Texto: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Introdução: A força centrífuga é um elemento importante na qualidade de força. O desenvolvimento da força centrífuga muscular pode, efetivamente, aumentar a estabilidade dos membros inferiores e reduzir o risco de lesões. Objetivo: Explorar as características da força contrátil do músculo flexor no treinamento de força e de reabilitação, e os músculos extensores da junta do joelho em atletas com diferentes velocidades de força centrípeta. Métodos: O grupo muscular da articulação do joelho de oito saltadores de primeiro escalão do sexo masculino, e oito saltadores de segundo escalão do sexo masculino foi testado por contração centrífuga isocinética. A velocidade de teste angular foi de 60 °/s, 120 °/s, 240 °/s, e os índices incluíram torque de pico, torque de pico relativo (torque de pico/peso corporal) e a razão extensão-flexão de torque de pico. Resultados: Na contração centrífuga do grupo muscular da articulação do joelho (P < 0,05), os saltadores de segundo escalão devem aumentar a habilidade do grupo muscular flexor do joelho da perna de arranque. No caso da contração centrífuga de velocidade constante (P < 0,01), o tempo de arranque deve ser reduzido, ou seja, a velocidade de arranque deve ser acelerada. Conclusões: A diferença nos resultados dos testes isocinéticos do músculo da junta do joelho é uma das razões para a diferença na contratilidade muscular flexor e extensor da junta do joelho sob forças de velocidade diferentes em saltadores. Nível de evidência II; Estudos terapêuticos – investigação de resultados de tratamento.

ABSTRACT Introduction: Centrifugal strength is an important element for strength quality. Developing muscle centrifugal strength can effectively increase the stability of lower limbs and reduce the risk of injury. Objective: To explore the characteristics of contractile force of flexor ahods in strength training and rehabilitation training, and the extensor muscles of the knee joint in athletes with different speeds of centripetal force. Methods: The knee joint muscle group of 8 first-level male high jumpers and 8 second-level male high jumpers were tested by isokinetic centrifugal contraction; the angular test velocity was 60 °/s, 120 °/s, 240 °/s, and the indexes included peak torque, relative peak torque (peak torque/body weight), and the peak torque flexural extension ratio. Results: With the centrifugal contraction of the knee joint muscle group (P < 0.05), the second-level high jumpers should increase the ability of the knee flexor muscle group of the take-off leg. In the case of constant velocity centrifugal contraction (P < 0.01), taking off time must be reduced, that is, taking off speed must be accelerated. Conclusions: The difference in the knee joint muscle isokinetic test results is one of the reasons for the difference in knee joint flexor and extensor muscle contractility under the different speed forces of high jumpers. Level of evidence II; Therapeutic studies - investigation of treatment results.

Resumen Introducción: La fuerza centrífuga es un elemento importante en la calidad de fuerza. El desarrollo de la fuerza centrífuga muscular puede efectivamente aumentar la estabilidad de los miembros inferiores y reducir el riesgo de lesiones. Objetivo: Explorar las características de la fuerza contráctil del músculo flexor en el entrenamiento de fuerza y de rehabilitación, y los músculos extensores de la articulación de la rodilla en atletas con diferentes velocidades de fuerza centrípeta. Métodos: Se testó el grupo muscular de la articulación de la rodilla de 8 saltadores de primer escalafón del sexo masculino, y 8 saltadores de segundo escalafón del sexo masculino por contracción centrífuga isocinética. La velocidad de prueba angular fue de 60 °/s, 120 °/s, 240 °/s, y los índices incluyeron torque de pico, torque de pico relativo (torque de pico/peso corporal) y la razón extensión-flexión de torque de pico. Resultados: En la contracción centrífuga del grupo muscular de la articulación de la rodilla (P<0,05), los saltadores de segundo escalafón deben aumentar la habilidad del grupo muscular flexor de la rodilla de la pierna de arrancada. En el caso de la contracción centrífuga de velocidad constante (P<0,01), el tiempo de arrancada debe reducirse, o sea, la velocidad de arrancada debe acelerar. Conclusiones: La diferencia en los resultados de las pruebas isocinéticas del músculo de la articulación de la rodilla es una de las razones para la diferencia en la contractilidad muscular flexor y extensor de la articulación de la rodilla bajo fuerzas de velocidad diferentes en saltadores. Nivel de evidencia II; Estudios terapéuticos – investigación de resultados de tratamiento.

https://doi.org/10.1590/1517-8692202127072021_0375

14.

Baryon Acoustic Oscillations from Integrated Neutral Gas Observations: an instrument to observe the 21cm hydrogen line in the redshift range 0.13 < z < 0.45 – status update
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WUENSCHE, CARLOS A.

; ABDALLA, ELCIO

; ABDALLA, FILIPE

; BAROSI, LUCIANO

; WANG, BIN

; AN, RUI

; BARRETOS, JOÃO A. M.

; BATTYE, RICHARD

; BRITO, FRANCISCO A.

; BROWNE, IAN

; CORREIA, DANIEL

; COSTA, ANDRÉ A.

; DELABROUILLE, JACQUES

; DICKINSON, CLIVE

; FENG, CHANG

; FERREIRA, ELISA G. M.

; FORNAZIER, KARIN

; DE GASPERIS, GIANCARLO

; GUTIERREZ, PRISCILA

; HARPER, STUART

; LANDIM, RICARDO G.

; LICCARDO, VINCENZO

; MA, YIN-ZHE

; MACHADO, TELMO

; MAFFEI, BRUNO

; MARINS, ALESSANDRO

; MENDES, MILENA M. M.

; MERICIA, EDUARDO

; MONSTEIN, CHRISTIAN

; MOTTA, PABLO

; NOVAES, CAMILA

; OTOBONE, CARLOS H.

; PEEL, MICHAEL

; QUEIROZ, AMILCAR R.

; RADCLIFFE, CHRISTOPHER

; REMAZEILLES, MATHIEU

; RIBEIRO, RAFAEL M. G.

; SANG, YU

; SANTOS, JULIANA F. R.

; SANTOS, LARISSA

; SANTOS, MARCELO. V.

; SHAN, CHENXI

; SILVA, GUSTAVO B.

; VIEIRA, FREDERICO

; VIEIRA, JORDANY

; VILLELA, THYRSO

; XIAO, LINFENG

; YANG, WEIQIANG

; ZHANG, JIAJUN

; ZHANG, XUE

; ZHU, ZENGHAO

Anais da Academia Brasileira de Ciências

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2021, Volume 93 elocation e20201096

Resumo: En | Texto: En | PDF: En

Abstract BINGO (BAO from Integrated Neutral Gas Observations) is a unique radio telescope designed to map the intensity of neutral hydrogen distribution at cosmological distances, making the first detection of Baryon Acoustic Oscillations (BAO) in the frequency band 980 MHz - 1260 MHz, corresponding to a redshift range 0.127 < z < 0.449. BAO is one of the most powerful probes of cosmological parameters and BINGO was designed to detect the BAO signal to a level that makes it possible to put new constraints on the equation of state of dark energy. The telescope will be built in Paraíba, Brazil and consists of two \thicksim 40m mirrors, a feedhorn array of 50 horns, and no moving parts, working as a drift-scan instrument. It will cover a 15 ^{\circ} ∘ declination strip centered at \sim \delta ∼ δ =-15 ^{\circ} ∘, mapping \sim ∼ 5400 square degrees in the sky. The BINGO consortium is led by University of São Paulo with co-leadership at National Institute for Space Research and Campina Grande Federal University (Brazil). Telescope subsystems have already been fabricated and tested, and the dish and structure fabrication are expected to start in late 2020, as well as the road and terrain preparation.

https://doi.org/10.1590/0001-3765202120201096 413 downloads

15.

Poricoic acid A induces apoptosis and autophagy in ovarian cancer via modulating the mTOR/p70s6k signaling axis
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Ma, Rui

; Zhang, Zhenhua

; Xu, Jin

; Liang, Xueqi

; Zhao, Qiang

Brazilian Journal of Medical and Biological Research

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2021, Volume 54 Nº 12 elocation e11183

Resumo: En | Texto: En | PDF: En

Due to the high mortality and rapid disease progression, ovarian cancer remains one of the most common malignancies threatening the health of women. The present study was conducted to explore the anticancer effects and the underlying mechanisms of poricoic acid A (PAA), the main components of Poria cocos, on ovarian cancer. We investigated the anticancer effects of different concentrations of PAA in the SKOV3 cell line. Cell viability and proliferation were examined by CCK-8 assay. Cellular migration and invasion were assessed by the scratch and Transwell migration assays, respectively. The effect of PPA on cell apoptosis was measured by flow cytometry and caspase-3/8/9 colorimetric assay. Western blot was performed to detect protein level changes related to apoptosis and mTOR signaling pathways. The in vivo anticancer effect of PAA was evaluated using xenograft tumorigenesis model in nude mice. Our results showed that PAA suppressed SKOV3 cellular viability, migration, and invasion in a dosage-dependent manner. Flow cytometry results demonstrated PAA treatment could induce SKOV3 cell apoptosis. In addition, increased ratio of LC3-II/LC3-I (a marker for autophagosome formation) was observed after PAA treatment, as well as inhibition of m-TOR and p70s6k phosphorylation. In nude mice, PAA treatment reduced the xenograft tumor weight by 70% (P<0.05). In conclusion, our data suggested that PAA induced apoptosis and autophagy in ovarian cancer via modulating the mTOR/p70s6k signaling axis.

https://doi.org/10.1590/1414-431x2021e11183 166 downloads

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