Abstract Introduction For a successful implant-supported rehabilitation and long-term maintenance in the anterior maxilla, it is imperative that the patient possesses adequate bone quality and quantity. Objective The aim of this study was to assess bone gain and stability following reconstruction with titanium mesh in the anterior region of the maxilla, irrespective of the biomaterial used for bone augmentation. Material and method Patient follow-up data were obtained from the Faculty's follow-up database. The focus was on individuals who had undergone rehabilitation in the anterior maxilla, previously augmented with bone grafts and growth factors in conjunction with titanium mesh. This retrospective cohort study involved at least 10 patients who met the inclusion criteria. Following an initial review of medical records, the eligible patients were invited for evaluation appointments. Result Out of 39 patients who had undergone guided bone regeneration surgery using titanium mesh, 12 met the inclusion criteria. As such, 17 sites suitable for the study were obtained, from which total of 276 tomographic measurements were then taken. There was a noticeable increase in thickness at all assessment levels relative to the bone crest apex, both immediately (T1) and six months post-surgery (T2), compared to baseline measurements (T0). Moreover, bone thickness tended to increase with the proximity to the apical level. Conclusion The use of titanium mesh combined with various biomaterials has yielded favorable outcomes in terms of augmenting bone volume. implantsupported implant supported longterm long term maxilla quantity augmentation followup follow up Facultys Faculty s database 1 criteria records appointments 3 such 27 taken apex T1 T (T1 postsurgery post T2, T2 , (T2) T0. T0 . (T0) Moreover level volume 2 (T (T2 (T0

Resumo Introdução Para o sucesso da reabilitação com implantes suportados e da manutenção dos resultados a longo prazo na região anterior da maxila, é imperativo que o paciente possua qualidade e quantidade óssea adequadas. Objetivo O objetivo deste estudo foi avaliar o ganho e a estabilidade óssea após a reconstrução com malha de titânio na região anterior da maxila, independentemente do biomaterial utilizado para o aumento ósseo. Material e método Utilizamos dados de pacientes oriundos do banco de dados da Faculdade, focando em indivíduos que haviam passado por reabilitação na maxila anterior previamente aumentada com enxertos ósseos e fatores de crescimento em conjunto com malha de titânio. Este estudo de coorte retrospectivo envolveu pelo menos 10 pacientes que atenderam aos critérios de inclusão. Resultado De um total de 39 registros no banco de dados de pacientes que passaram por cirurgia de regeneração óssea guiada utilizando malha de titânio, doze atenderam aos critérios de inclusão, enquanto os demais foram excluídos. Assim, foram obtidos 17 sítios adequados para o estudo, envolvendo 12 pacientes e um total de 276 medições tomográficas. Houve um aumento notável na espessura óssea em todos os níveis de avaliação em relação ao ápice da crista óssea, tanto imediatamente (T1) quanto seis meses após a cirurgia (T2), em comparação com as medições iniciais (T0). Além disso, a espessura óssea tende a aumentar com a proximidade do nível apical. Conclusão A utilização da malha de titânio combinada com vários biomateriais resultou em resultados favoráveis em termos de aumento do volume ósseo. adequadas ósseo Faculdade 1 inclusão 3 excluídos Assim 27 tomográficas T1 T (T1 T2, T2 , (T2) T0. T0 . (T0) disso apical 2 (T (T2 (T0

Abstract Introduction The control of peri-implant biofilm plays a crucial role in the long-term success of dental implants. Topical treatment alternatives aimed at reducing or preventing biofilm accumulation around the implant's prosthetic components are critical for the success of the treatment. Objective The present study has clinically evaluated the effects of an oxygen-releasing mouthwash (BlueM®) over a 90-day period, following implant placement surgery. Material and method Inclusion and exclusion criteria allowed for the selection of participants who, after implant placement, were randomized into two groups: the control group, which used a 0.12% chlorhexidine mouthwash, and the BlueM group, which received the oxygen-releasing BlueM mouthwash. All participants were instructed to rinse twice daily for the first 10 days and once a day up to 30 days post-surgery. Clinical parameters were evaluated, including the visible plaque index (VP), marginal bleeding (MB), presence of calculus (PC), suppuration (SUP), mucositis (MU), and the extent of keratinized gingiva (KG). These data were collected at the time of suture removal (baseline). At 90 days postoperative, in addition to the aforementioned parameters, probing depth (PD), bleeding on probing (BOP), and the presence of peri-implantitis (PI) were evaluated. Result Nine patients were included in this study, with a total of 38 implants. The BlueM group demonstrated a reduction in MB and an even greater reduction in VP. The other assessed parameters showed similar results to those in the control group. Conclusion BlueM mouthwash has clinical benefits for biofilm control and gingival inflammation reduction. periimplant peri longterm long term implants s oxygenreleasing oxygen releasing BlueM® (BlueM® 90day period surgery who groups 012 0 12 0.12 1 3 postsurgery. postsurgery post post-surgery VP, VP , (VP) MB, (MB) PC, PC (PC) SUP, SUP (SUP) MU, MU (MU) KG. KG . (KG) baseline. baseline (baseline) 9 postoperative PD, PD (PD) BOP, BOP (BOP) periimplantitis implantitis PI (PI (BlueM 01 0.1 (VP (MB (PC (SUP (MU (KG (baseline (PD (BOP 0.

Resumo Introdução O controle do biofilme peri-implantar desempenha um papel importante no sucesso a longo prazo dos implantes dentários. Alternativas de tratamento tópico visando a diminuição ou a prevenção do acumulo de biofilme ao redor dos componentes protéticos do implante é um fator crucial para a sucesso do tratamento. Objetivo Assim, o presente estudo avaliou clinicamente no período de 90 dias, os efeitos de um enxaguatório bucal liberador de oxigênio (BlueM®) após cirurgia de instalação de implante. Material e método Após a instalação dos implantes, os pacientes foram randomizados em dois grupos: grupo controle utilizaram enxaguatório bucal com clorexidina 0,12% e grupo BlueM utilizaram enxaguatório bucal liberador de oxigênio (BlueM). Todos participantes foram orientados a realizarem os bochechos 2 x/dia nos primeiros 10 dias, e 1 x/dia até completar 30 dias da cirurgia. Parâmetros clínicos foram avaliados: índice de placa visível (PV), sangramento marginal (SM), presença de cálculo (PC), presença de supuração (SUP), presença de mucosite (MU), extensão da faixa de gengiva ceratinizada (GC) realizados no ato da remoção da sutura (Baseline). No período de 90 dias do pós-operatório foram realizados os exames de profundidade de sondagem (PS), sangramento à sondagem (SS) e de presença de perimplantite (Pi). Resultado 9 pacientes foram incluídos neste estudo totalizando 38 implantes instalados. O grupo BlueM apresentou redução no SM, maior redução de PV. Os demais parâmetros avaliados foram semelhantes com o grupo controle. Conclusão Pode-se concluir que o enxaguatório BlueM demonstrou benefícios clínicos no controle do biofilme e no controle da inflamação gengival. periimplantar peri implantar dentários Assim BlueM® (BlueM® grupos 012 0 12 0,12 BlueM. . (BlueM) xdia x dia 3 PV, PV , (PV) SM (SM) PC, PC (PC) SUP, SUP (SUP) MU, MU (MU) GC (GC Baseline. Baseline (Baseline) pósoperatório pós operatório PS, PS (PS) SS (SS Pi. Pi (Pi) instalados Podese Pode se gengival (BlueM 01 0,1 (PV (SM (PC (SUP (MU (Baseline (PS (Pi 0,

Abstract The aim of this study was to evaluate the effects of long-term alendronate administration on bone repair and mineralization around osseointegrated implants in rats. A total of 160 female Wistar rats were randomly assigned to two groups: the control group (CTL) and the alendronate group (ALD). The ALD group received a subcutaneous injection of sodium alendronate (1 mg/kg/week), while the CTL group received weekly injections of saline solution. After 120 days of treatment, a bilateral implant was placed in the tibia of each rat. Ten rats from each group were euthanized at 5, 10, 15, 20, 25, 30, 45, or 60 days post-surgery. Picro-sirius red staining was utilized to assess the distribution and arrangement of collagen fibers near the implant threads. Bone mineralization mapping of the native bone adjacent to the implant was performed using images obtained through scanning electron microscopy (SEM) across all follow-up periods. SEM-based mineralization mapping revealed an increase in both the degree and homogeneity of bone mineralization in the ALD group compared to the CTL group. Alendronate administration affected collagen arrangement and distribution, leading to a connective tissue with reduced organization and thinner collagen fiber bundles. In conclusion, the findings demonstrated that alendronate administration resulted in a higher degree and homogeneity of bone mineralization, accompanied by reduced collagen content and organization, suggesting an impairment in bone remodeling around dental implants. longterm long term 16 groups (CTL ALD. . (ALD) 1 ( mg/kg/week, mgkgweek mg/kg/week , mg kg week mg/kg/week) solution 12 treatment rat 5 10 15 20 25 30 45 6 postsurgery. postsurgery post surgery. surgery post-surgery Picrosirius Picro sirius threads SEM (SEM followup follow up periods SEMbased based bundles conclusion (ALD 2 3 4

Resumo O objetivo deste estudo foi avaliar o uso prolongado do alendronato no reparo ósseo e na mineralização ao redor de implantes osseointegrados em ratos. Este estudo incluiu 160 ratas Wistar, distribuídas aleatoriamente em dois grupos: grupo controle (CTL) e grupo alendronato (ALD). As ratas do grupo ALD receberam injeção subcutânea de alendronato de sódio (1 mg/kg/semana), enquanto as do grupo CTL receberam injeção de solução salina uma vez por semana. Após 120 dias de tratamento, um implante foi colocado bilateralmente na tíbia dos animais. Dez ratas por grupo foram sacrificadas aos 5, 10, 15, 20, 25, 30, 45 ou 60 dias após a cirurgia. A coloração Picro-Sirius Red foi utilizada para avaliar a distribuição e disposição das fibras colágenas na área próxima às roscas do implante. O mapeamento da mineralização óssea no osso nativo adjacente ao implante foi realizado a partir de imagens adquiridas por microscopia eletrônica de varredura (MEV) em todos os períodos de acompanhamento. As análises das imagens de MEV demonstraram aumento no grau de mineralização óssea e maior homogeneidade no grupo ALD em comparação ao grupo CTL. A administração de alendronato interferiu no arranjo e no padrão de distribuição do colágeno, resultando em tecido conjuntivo com menor grau de organização e feixes de fibras colágenas mais finos. Em conclusão, os resultados deste estudo mostraram que a administração de alendronato levou ao aumento do grau e da homogeneidade da mineralização óssea e à redução do conteúdo e da organização do colágeno em comparação ao grupo CTL, sugerindo um comprometimento da remodelação óssea ao redor dos implantes dentários. ratos 16 Wistar grupos (CTL ALD. . (ALD) 1 ( mg/kg/semana, mgkgsemana mg/kg/semana , mg kg semana mg/kg/semana) 12 tratamento animais 5 10 15 20 25 30 4 6 cirurgia PicroSirius Picro Sirius (MEV acompanhamento finos conclusão dentários (ALD 2 3

Abstract Introduction Periodontitis is a known risk factor for hypertensive subjects, with evidence suggesting that general inflammation is linked to both disorders. Objective To investigate the influence of non-surgical periodontal therapy (NSPT) on the oral health-related quality of life in subjects with refractory arterial hypertension and periodontitis. Material and method 27 patients with refractory hypertension and stage III and/or IV, grade B periodontitis experienced NSPT. Clinical periodontal parameters, including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BoP), and plaque index (PI), were assessed. Quality of life was assessed by the Oral Health Impact Profile questionnaire (OHIP-14). During the follow-up, 24 participants were evaluated at 90 days, and 22 participants at 180 days. Data were analyzed statistically (α=5%). Result Substantial enhancements were detected in periodontal parameters PD, BoP, and PI across the evaluated periods. Additionally, the sum of OHIP-14 score was significantly reduced at 180 days post-treatment. Notably, responses related to the subdomains "physical pain," "psychological disability," and "social disability" indicated an improvement in quality of life after 180 days of treatment. Conclusion: These findings highlight the positive impact of NSPT on the quality of life of patients with refractory arterial hypertension and periodontitis. disorders nonsurgical non surgical (NSPT healthrelated health 2 andor or IV PD , (PD) CAL, CAL (CAL) BoP (BoP) PI, (PI) OHIP14. OHIP14 OHIP 14 . (OHIP-14) followup, followup follow up, up follow-up 9 18 α=5%. α5 α α=5% 5 (α=5%) periods Additionally OHIP-1 posttreatment. posttreatment post treatment post-treatment Notably physical pain, pain psychological disability, disability social Conclusion (PD (CAL (BoP (PI OHIP1 1 (OHIP-14 α=5 (α=5% OHIP- (OHIP-1 α= (α=5 (OHIP- (α= (OHIP (α

Resumo Introdução A periodontite é um indicador de risco para pacientes hipertensos, e há indicações de que a inflamação sistêmica está associada a ambas as condições. Objetivo Investigar o impacto da terapia periodontal não cirúrgica (TPNC) na qualidade de vida relacionada à saúde bucal em pacientes com hipertensão arterial refratária e periodontite. Material e método 27 participantes com hipertensão arterial refratária e periodontite estágio III e IV, grau B receberam a TPNC. Os parâmetros clínicos periodontais avaliados foram profundidade de sondagem (PS), nível de inserção clínica (NIC), sangramento à sondagem (SS) e índice de placa (IP). A qualidade de vida foi mensurada através do questionário Oral Health Impact Profie (OHIP-14). No período de acompanhamento foram avaliados vinte e quatro participantes aos 90 dias e vinte e dois aos 180 dias. Os dados foram submetidos a análise estatística (α=5%). Resultado Os parâmetros clínicos periodontais PS, SS e IP apresentaram diferenças estatisticamente significativas entre os períodos avaliados. A pontuação da soma dos escores do OHIP-14 foi significativamente menor aos 180 dias após o tratamento. Os resultados demonstraram que houve melhora da qualidade de vida nos subdomínios de “dor física”, “deficiência psicológica” e “deficiência social” aos 180 dias pós-tratamento. Conclusão esses achados mostram o impacto positivo da TPNC na qualidade de vida de pacientes com hipertensão arterial refratária e periodontite. hipertensos condições (TPNC 2 IV PS , (PS) NIC, NIC (NIC) (SS IP. . (IP) OHIP14. OHIP14 OHIP 14 (OHIP-14) 9 18 α=5%. α5 α α=5% 5 (α=5%) OHIP-1 tratamento dor física, física física” deficiência psicológica social póstratamento. póstratamento pós pós-tratamento (PS (NIC (IP OHIP1 1 (OHIP-14 α=5 (α=5% OHIP- (OHIP-1 α= (α=5 (OHIP- (α= (OHIP (α

Abstract Objectives This study aimed to investigate the osseointegration of titanium (Ti) implants with micro-nano textured surfaces functionalized with strontium additions (Sr) in a pre-clinical rat tibia model. Methodology Ti commercially pure (cp-Ti) implants were installed bilaterally in the tibia of 64 Holtzman rats, divided into four experimental groups (n=16/group): (1) Machined surface - control (C); (2) Micro-nano textured surface treatment (MN); (3) Micro-nano textured surface with Sr2+ addition (MNSr); and (4) Micro-nano textured surface with a higher complementary addition of Sr2+ (MNSr+). In total, two experimental euthanasia periods were assessed at 15 and 45 days (n=8/period). The tibia was subjected to micro-computed tomography (μ-CT), histomorphometry with the EXAKT system, removal torque (TR) testing, and gene expression analysis by PCR-Array of 84 osteogenic markers. Gene expression and protein production of bone markers were performed in an in vitro model with MC3T3-E1 cells. The surface characteristics of the implants were evaluated by scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), and laser scanning confocal microscopy. Results SEM, confocal, and EDS analyses demonstrated the formation of uniform micro-nano textured surfaces in the MN group and Sr addition in the MNSr and MNSr+ groups. TR test indicated greater osseointegration in the 45-day period for treated surfaces. Histological analysis highlighted the benefits of the treatments, especially in cortical bone, in which an increase in bone-implant contact was found in groups MN (15 days) and MNSr (45 days) compared to the control group. Gene expression analysis of osteogenic activity markers showed modulation of various osteogenesis-related genes. According to the in vitro model, RT-qPCR and ELISA demonstrated that the treatments favored gene expression and production of osteoblastic differentiation markers. Conclusions Micro-nano textured surface and Sr addition can effectively improve and accelerate implant osseointegration and is, therefore, an attractive approach to modifying titanium implant surfaces with significant potential in clinical practice. (Ti micronano micro nano (Sr preclinical pre cpTi cp (cp-Ti 6 rats n=16/group n16group ngroup n 16 (n=16/group) 1 (1 C (C) 2 (2 Micronano Micro (MN) 3 (3 Sr2 (MNSr) 4 (4 MNSr+. . (MNSr+) total n=8/period. n8period nperiod n=8/period 8 (n=8/period) microcomputed computed μCT, μCT μ CT , (μ-CT) system (TR testing PCRArray PCR Array MC3T3E1 MCTE MC3T3 E1 MC T E MC3T3-E cells SEM (SEM) energydispersive energy dispersive EDS, (EDS) 45day day boneimplant osteogenesisrelated osteogenesis related genes RTqPCR RT qPCR is therefore practice (n=16/group ( (C (MN (MNSr (MNSr+ (n=8/period (μ-CT MC3T3E MCT MC3T (SEM (EDS

Abstract The aim of this systematic review was to answer the following question: “Does alendronate, a nitrogen-containing bisphosphonate, improve or impair alveolar socket healing after tooth extraction in animal models”? To this end, a systematic review of the literature was carried out in PubMed, Scopus, LILACS, Web of Science, as well as in the gray literature up to May 2023. Preclinical studies that evaluated alveolar healing after tooth extraction and the intake of sodium alendronate compared with placebo were included. Two investigators were responsible for screening the articles independently, extracting the data, and assessing their quality through the SYRCLE’s RoB tool for randomized trials in animal studies. The study selection process, study characteristics, risk of bias in studies, impact of alendronate on bone healing, and certainty of evidence were described in text and table formats. Methodological differences among the studies were restricted to the synthesis methods. The synthesis of qualitative results followed the Synthesis Without Meta-analysis (SWiM) reporting guideline. From the 19 included studies, five were considered to have low risk, three were of unclear risk, and eleven presented a high risk of bias. The studies were considered heterogeneous regarding alendronate posology, including its dosage and route of administration. Furthermore, a variety of animal species, different age ranges, diverse teeth extracted, and exposure or not to ovariectomy contributed to the lack of parity of the selected studies. Our results indicated that alendronate monotherapy negatively affects the early phase of wound healing after tooth extraction in preclinical studies, suggesting that the bone resorption process after tooth extraction in animals treated with alendronate might impair the bone healing process of the extraction socket. In conclusion, alendronate administration restrains bone resorption, thereby delaying alveolar socket healing . Future studies should be conducted to validate these findings and to better understand the effects of alendronate therapy on oral tissues. question Does nitrogencontaining nitrogen containing bisphosphonate models models” end PubMed Scopus LILACS Science 2023 independently data SYRCLEs SYRCLE s characteristics formats methods Metaanalysis Meta analysis SWiM (SWiM guideline 1 posology Furthermore species ranges extracted conclusion tissues 202 20 2

Abstract Studies have highlighted numerous benefits of ozone therapy in the field of medicine and dentistry, including its antimicrobial efficacy against various pathogenic microorganisms, its ability to modulate the immune system effectively, reduce inflammation, prevent hypoxia, and support tissue regeneration. However, its effects on dental extraction healing remain to be elucidated. Objective Therefore, this study aimed to evaluate the effects of systemically administered ozone (O3) at different doses in the healing of dental extraction sockets in rats. Methodology To this end, 72 Wistar rats were randomly divided into four groups after extraction of the right upper central incisor: Group C – control, no systemic treatment; Group OZ0.3 – animals received a single dose of 0.3 mg/kg O3; Group OZ0.7 – a single dose of 0.7 mg/kg O3; and Group OZ1.0 – a single dose of 1.0 mg/kg O3, intraperitoneally. In total, six animals from each group were euthanized at 7, 14, and 21 days after the commencement of treatment. Bone samples were harvested and further analyzed by descriptive histology, histomorphometry, and immunohistochemistry for osteocalcin (OCN) and tartrate-resistant acid phosphatase (TRAP) protein expression. Results All applied doses of O3 were shown to increase the percentage of bone tissue (PBT) after 21 days compared to group C. After 14 days, the OZ0.7 and OZ1.0 groups showed significantly higher PBT when compared to group C. The OZ1.0 group presented the most beneficial results regarding PBT among groups, which denotes a dose-dependent response. OCN immunostaining was higher in all groups at 21 days. However, after seven and 14 days, the OZ1.0 group showed a significant increase in OCN immunostaining compared to C group. No differences in TRAP+ osteoclasts were found between groups and time points. Conclusion Therefore, O3 therapy at higher doses might be beneficial for bone repair of the alveolar socket following tooth extraction. dentistry microorganisms effectively inflammation hypoxia regeneration However elucidated Therefore O (O3 end 7 incisor control treatment OZ03 OZ OZ0 3 OZ0. 03 0 0. mgkg mg kg OZ07 07 OZ10 OZ1 OZ1. 10 1 1. intraperitoneally total 2 histology histomorphometry (OCN tartrateresistant tartrate resistant TRAP (TRAP expression (PBT dosedependent dependent response points (O