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Natural Durability of Mimosa tenuiflora and Mimosa caesalpiniaefolia Woods: Two Species from the Brazilian Dry Forest Woods
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Braga, Rodolpho Stephan Santos

; Melo, Rafael Rodolfo de

; Pimenta, Alexandre Santos

; Garlet, Alencar

; Canto, Juliana Lorensi do

; Medeiros Neto, Pedro Nicó de

Floresta e Ambiente

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2024, Volume 31 Nº 3 elocation e20240011

Resumo: En | Texto: En | PDF: En

Abstract Knowledge of the characteristics and durability of every wood species is essential so it can be better directed for the correct harnessing. In a wood rot field test, this work aimed to determine the natural durability of timber species from the Brazilian dry forest, Mimosa tenuiflora and Mimosa caesalpiniaefolia . The slats were buried in the ground up to half their length and distributed in the field according to a completely randomized experimental design. Annual assessments were performed to determine the deterioration rate. Furthermore, mass loss and condensed tannin content were quantified. The condensed tannin content found for the species was 4.40% for M. caesalpiniaefolia and 3.99% for M. tenuiflora . The results of 3 years of evaluation of the test make it possible to understand that both species presented durable wood, where M. caesalpiniaefolia presented a deterioration rate of 77.27%, while M. tenuiflora presented 66.27%, making them of high natural durability. harnessing forest design Furthermore quantified 440 4 40 4.40 M 399 99 3.99 7727 77 27 77.27% 6627 66 66.27% 44 4.4 39 9 3.9 772 7 2 77.27 662 6 66.27 4. 3. 77.2 66.2 77. 66.

https://doi.org/10.1590/2179-8087-floram-2024-0011

Identification of insect body fragments found in fecal samples of Sapajus nigritus (Primates: Cebidae) from five Conservation Units in Rio de Janeiro state, Brazil Primates (Primates Cebidae state
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MEDEIROS, JULIANA DE M.

; PENEDO, DIEGO M.

; RESENDE, ANDRÉ LUIS S.

; NOGUEIRA, DENISE M.

Anais da Academia Brasileira de Ciências

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2024, Volume 96 Supl. 1 elocation e20231272

Resumo: En | Texto: En | PDF: En

Abstract Sapajus nigritus (Primates: Cebidae) is endemic of the Atlantic Forest, occurring from the Southeastern and Southern regions of Brazil to Northeastern Argentina. This species plays a role in two ecosystem services: seed dispersal through endozoochory and insect population control in agricultural plantations and forest environments. Fruits and invertebrates represent approximately 90% of their diet, and there is a large number of insects in the diet of S. nigritus in urban and conserved areas. However, it is known that insect diversity decreases in anthropized environments. Our objective was to identify the insects present in the fecal samples of S. nigritus from five Conservation Units in Rio de Janeiro state, Brazil. We aim to estimate the percentage of each taxon of insects found in feces either, hypothesizing that there are a greater variety of insect species in the diet of S. nigritus that inhabit preserved forested areas. A fecal screening was conducted using a light microscopy and the insects were identified based on their external morphology. Insect fragments were found in eight out of ten fecal samples of S. nigritus, revealing that they belonged to insects from five orders: Hymenoptera, Hemiptera, Orthoptera, Coleoptera and Blattodea, suggesting a good conservation status of the sampling areas. Primates (Primates Cebidae Forest Argentina services environments 90 S areas However state either morphology orders Hymenoptera Hemiptera Orthoptera Blattodea 9

https://doi.org/10.1590/0001-3765202420231272

Physiological aspects and production of coriander using nutrient solutions prepared in different brackish waters
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Navarro, Franz E. C.

; Santos Júnior, José A.

; Martins, Juliana B.

; Cruz, Ruana I. F.

; Silva, Manassés M. da

; Medeiros, Salomão de S.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Nov 2022, Volume 26 Nº 11 Páginas 831 - 839

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO A análise da fluorescência da clorofila é uma das muitas formas de quantificar o dano salino no desempenho fotossintético e na produção agrícola. Assim, o presente estudo teve como objetivo avaliar a eficiência fotoquímica e a produção do coentro, cultivar Verdão em função dos níveis de condutividades elétricas da solução nutritiva e da natureza catiônica. O delineamento experimental foi em blocos ao acaso, em esquema fatorial 4 × 3, com quatro repetições. Os tratamentos consistiram na exposição das plantas a quatro condutividades elétricas das soluções nutritivas (CEsn = 1,6; 3,2; 4,8 e 6,4 dS m-1) e três tipos de águas de diferentes naturezas catiônicas (Na+; Ca2+; Mg2+), preparadas com a solubilização de diferentes sais - NaCl, CaCl2.2H2O e MgCl2.6H2O em água de abastecimento da UFRPE (CEa = 0,12 dS m-1). O estudo foi realizado em casa de vegetação entre novembro e dezembro de 2019 no Laboratório de Fertirrigação e Salinidade do Departamento de Engenharia Agronômica da Universidade Federal Rural de Pernambuco. O aumento da condutividade elétrica da solução nutritiva afetou os centros de reação, a atividade fotoquímica, a eficiência da carboxilação e resultou na redução da condutância estomática, da taxa de assimilação de CO2 e, portanto, na produção de biomassa do coentro. Diferentes prevalências catiônicas na água proporcionam diferenças na intensidade do dano salino, sobretudo com o aumento da concentração.

ABSTRACT The analysis of chlorophyll fluorescence is one of many ways to quantify the salt damage to photosynthetic performance and crop production. Thus, the present study aimed to evaluate the photochemical efficiency and production of coriander, cultivar ‘Verdão’, as a function of the electrical conductivity levels of the nutrient solution and the cationic nature. The experimental design was in randomized blocks, in a 4 × 3 factorial scheme, with four replicates. The treatments consisted of four electrical conductivities of the nutrient solutions (ECns = 1.6, 3.2, 4.8, and 6.4 dS m-1) and three kinds of water of different cationic natures (Na+; Ca2+; Mg2+), which were prepared with the dissolution of different salts - NaCl, CaCl2.2H2O, and MgCl2.6H2O in supply water (ECw = 0.12 dS m-1), that is, three predominant cationic natures. The study was carried out in a greenhouse between November and December 2019 at the Fertigation and Salinity Laboratory of the Agricultural Engineering Department of the Universidade Federal Rural de Pernambuco. It was found that the increase in the electrical conductivity of the nutrient solution affected reaction centers, photochemical activity, and carboxylation efficiency and resulted in reductions in stomatal conductance, CO2 assimilation rate, and therefore, in the biomass production of coriander. Different cationic prevalence in water causes differences in the intensity of salt damage, especially with increasing concentration.

https://doi.org/10.1590/1807-1929/agriambi.v26n11p831-839

Binge drinking in Brazilian adolescents: results of a national household survey adolescents
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Medeiros, Pollyanna Fausta Pimentel de

; Valente, Juliana Y.

; Rezende, Leandro F. M.

; Sanchez, Zila M.

Cadernos de Saúde Pública

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2022, Volume 38 Nº 12 elocation e00077322

Resumo: Es En Pt | Texto: Es En Pt | PDF: Es | PDF: En | PDF: Pt

Los objetivos de este estudio fueron describir la prevalencia del consumo excesivo de alcohol (binge drinking) y otros niveles de consumo, así como analizar las características sociodemográficas asociadas al consumo excesivo de alcohol por adolescentes brasileños de entre los 15 y los 17 años. La encuesta domiciliaria transversal aplicó un cuestionario sobre consumo excesivo de alcohol (frecuencia semanal, dosis promedio consumida al día y consumo excesivo de alcohol) a 2.315 adolescentes de la Encuesta Nacional de Salud de 2019. Se realizaron un análisis descriptivo del consumo de alcohol desde las características sociodemográficas y un modelo de regresión logística multivariado. La prevalencia de consumo excesivo de alcohol en los últimos 30 días fue del 8,1% (IC95%: 6,2-10,3). Los adolescentes que no asistían a la escuela (OR = 2,8; IC95%: 1,6-5,0) tenían más probabilidades de consumir alcohol en exceso que los estudiantes que asistían a la escuela. El mayor nivel socioeconómico (medido por la escala de riqueza) también se asoció con mayores probabilidades de consumir alcohol en exceso en comparación con los adolescentes con un nivel socioeconómico más bajo (por ejemplo, quintil 5 vs. quintil 1: OR = 2,9; IC95%: 1,1-7,7). Las mujeres tenían menos probabilidades (OR = 0,5; IC95%: 0,2-0,8) de consumir alcohol en exceso que los varones. Se encontró una alta prevalencia de consumo excesivo de alcohol entre los adolescentes, especialmente entre los varones, que no asistían a la escuela y con un nivel socioeconómico más alto. El seguimiento de los niveles de consumo de alcohol en adolescentes puede aportar información importante para las políticas o acciones destinadas a la prevención del consumo excesivo de alcohol. binge drinking 1 años frecuencia semanal 2315 2 315 2.31 2019 multivariado 3 81 8 8,1 IC95% IC95 IC (IC95% 6,210,3. 62103 6,2 10,3 . 6 10 6,2-10,3) 2,8 28 1,65,0 1650 1,6 5,0 0 1,6-5,0 medido riqueza ejemplo vs 2,9 29 9 1,17,7. 1177 1,1 7,7 7 1,1-7,7) 0,5 05 0,20,8 0208 0,2 0,8 0,2-0,8 varones alto 231 31 2.3 201 8, IC9 (IC95 210 6,210,3 6210 62 6, 103 10, 6,2-10,3 2, 65 1,65, 165 16 1, 50 5, 1,6-5, 1,17,7 117 11 77 7, 1,1-7,7 0, 20 0,20, 020 02 08 0,2-0, 23 2. (IC9 21 6,210, 621 6,2-10, 1,65 1,6-5 1,17, 1,1-7, 0,20 0,2-0 (IC 6,210 6,2-10 1,6- 1,17 1,1-7 0,2- 6,21 6,2-1 1,1- 6,2-

This study aimed to estimate the prevalence of binge drinking and other alcohol consumption patterns and analyze the sociodemographic characteristics associated with binge drinking among Brazilian adolescents aged 15 to 17 years. A cross-sectional household survey including 2,315 adolescents from the 2019 Brazilian National Health Survey who answered a questionnaire on alcohol consumption (weekly frequency, average amount consumed in a drinking day, and binge drinking). A descriptive analysis of alcohol consumption by sociodemographic characteristics and a multivariable logistic regression model were performed. The prevalence of binge drinking (past 30 days) was 8.1% (95%CI: 6.2-10.3). Adolescents who did not attend school (OR = 2.8; 95%CI: 1.6-5.0) had greater odds of binge drinking than adolescents attending school. Higher socioeconomic status (measured via wealth score) was also associated with higher odds of binge drinking compared with lower socioeconomic status (e.g., 5th quintile vs. 1st quintile: OR = 2.9; 95%CI: 1.1-7.7). Girls had lower odds of binge drinking (OR = 0.5; 95%CI: 0.2-0.8) than boys. The prevalence of binge drinking among adolescents, especially boys, who did not attend school and had higher socioeconomic status was high. Monitoring alcohol consumption patterns in adolescents may provide important information on policies or actions to prevent binge drinking. 1 years crosssectional cross sectional 2315 2 315 2,31 201 weekly frequency day . drinking) performed past 3 days 81 8 8.1 95%CI 95CI CI 95 (95%CI 6.210.3. 62103 6.2 10.3 6 10 6.2-10.3) 2.8 28 1.65.0 1650 1.6 5.0 5 0 1.6-5.0 measured score e.g., eg e g (e.g. th vs st 2.9 29 9 1.17.7. 1177 1.1 7.7 7 1.1-7.7) 0.5 05 0.20.8 0208 0.2 0.8 0.2-0.8 boys high 231 31 2,3 20 8. 210 6.210.3 6210 62 6. 103 10. 6.2-10.3 2. 65 1.65. 165 16 1. 50 5. 1.6-5. e.g. (e.g 1.17.7 117 11 77 7. 1.1-7.7 0. 0.20. 020 02 08 0.2-0. 23 2, 21 6.210. 621 6.2-10. 1.65 1.6-5 e.g 1.17. 1.1-7. 0.20 0.2-0 6.210 6.2-10 1.6- 1.17 1.1-7 0.2- 6.21 6.2-1 1.1- 6.2-

Os objetivos deste estudo foram descrever a prevalência de consumo excessivo de álcool (binge drinking) e outros padrões de consumo e analisar as características sociodemográficas associadas ao consumo excessivo de álcool em adolescentes brasileiros de 15 a 17 anos. Nesta pesquisa domiciliar transversal, 2.315 adolescentes da Pesquisa Nacional de Saúde de 2019 responderam a um questionário sobre o consumo excessivo de álcool (frequência semanal, dose média consumida em um dia e consumo excessivo de álcool). Foram realizados uma análise descritiva do consumo de álcool por características sociodemográficas e um modelo de regressão logística multivariável. A prevalência de consumo excessivo de álcool nos últimos 30 dias foi de 8,1% (IC95%: 6,2-10,3). Os adolescentes que não frequentavam a escola (OR = 2,8; IC95%: 1,6-5,0) apresentaram maiores chances de consumo excessivo de álcool do que os alunos que frequentavam a escola. Um maior nível socioeconômico (medido por meio do escore de riqueza) também foi associado a maiores chances de consumo excessivo de álcool em comparação com adolescentes com menor nível socioeconômico (por exemplo, quintil 5 vs. quintil 1: OR = 2.9; IC95%: 1,1-7,7). Meninas tiveram menores chances (OR = 0,5; IC95%: 0,2-0,8) de consumo excessivo de álcool do que meninos. Observou-se uma alta prevalência de consumo excessivo de álcool entre adolescentes, principalmente entre meninos, que não frequentam a escola e com maior nível socioeconômico. O monitoramento de padrões de consumo de álcool em adolescentes pode fornecer informações importantes sobre políticas e ações para prevenção do consumo excessivo de álcool. binge drinking 1 anos transversal 2315 2 315 2.31 201 frequência semanal . álcool) multivariável 3 81 8 8,1 IC95% IC95 IC (IC95% 6,210,3. 62103 6,2 10,3 6 10 6,2-10,3) 2,8 28 1,65,0 1650 1,6 5,0 0 1,6-5,0 medido riqueza exemplo vs 2.9 29 9 1,17,7. 1177 1,1 7,7 7 1,1-7,7) 0,5 05 0,20,8 0208 0,2 0,8 0,2-0,8 meninos Observouse Observou se 231 31 2.3 20 8, IC9 (IC95 210 6,210,3 6210 62 6, 103 10, 6,2-10,3 2, 65 1,65, 165 16 1, 50 5, 1,6-5, 2. 1,17,7 117 11 77 7, 1,1-7,7 0, 0,20, 020 02 08 0,2-0, 23 (IC9 21 6,210, 621 6,2-10, 1,65 1,6-5 1,17, 1,1-7, 0,20 0,2-0 (IC 6,210 6,2-10 1,6- 1,17 1,1-7 0,2- 6,21 6,2-1 1,1- 6,2-

https://doi.org/10.1590/0102-311xen077322

Binge drinking in Brazilian adolescents: results of a national household survey
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Medeiros, Pollyanna Fausta Pimentel de

; Valente, Juliana Y.

; Rezende, Leandro F. M.

; Sanchez, Zila M.

Cadernos de Saúde Pública

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2022, Volume 38 Nº 12 elocation e00077322

Resumo: En Pt Es | Texto: En Pt Es | PDF: En | PDF: Pt | PDF: Es

https://doi.org/10.1590/0102-311xen077322

Forced Degradation Study and Development of a Stability-Indicating Method for Amoxicillin Capsules According to ANVISA Guidelines
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Gallo, Margareth B. C

; Nascimento, Diogo D. do

; Nunes, Nelson M

; Sousa, Flávia F. M. de

; Boniatti, Janine

; Aguiar, José L. N. de

; Medeiros, Juliana J. S

; Esteves, Alessandra L

; Guerra, Graça M. S

; Ferreira, Luiz E. M

; Regis, Lucas G. I

; Seiceira, Rafael C

; Moncorvo, Fabiana M. S. U

; Chaves, Marcelo H. C

Journal of the Brazilian Chemical Society

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Dez 2021, Volume 32 Nº 12 Páginas 2195 - 2205

Resumo: En | Texto: En | PDF: En

This study aimed to develop a reliable stability-indicating method (SIM) for amoxicillin 500 mg capsules (DP-drug product). A literature review addressing amoxicillin (AMX; DS-drug substance) forced degradation studies and the existing SIMs was conducted to verify the most significant outcomes. Subsequently, the forced degradation of DP and DS was carried out following the Brazilian Health Surveillance Agency (ANVISA) guidelines, including thermal degradation (dry and wet heat), acidic and alkaline hydrolyses, hydrogen peroxide oxidation, reaction with copper, and photodegradation. Both DS and DP were more susceptible to 0.015 M NaOH, resulting in approximately 50% degradation. AMX DS and DP were not significantly photodegraded, but some degradation products (PDegs) showed susceptibility to light exposure. Thermodegraded samples showing ≥ 10% degradation exhibited modified profiles in thermogravimetric (TG) and differential scanning calorimetric (DSC) analyses. The X-ray powder diffraction patterns (XRPD) of DS samples exposed to dry and wet heat displayed complete amorphization of AMX, attesting to the occurrence of physical degradation concomitantly with chemical degradation, which can alter the drug’s bioavailability. In contrast, the thermodegraded DP samples exhibited intact AMX crystals interspersed with the amorphous form, perhaps partly protected by the excipient. The validated SIM was able to detect and quantify about 80 PDegs.

https://doi.org/10.21577/0103-5053.20210111

O “Princípio de Pascal” nos livros do PNLD 2018: uma análise crítica multicontextual (histórica e conceitual)
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Hidalgo, Juliana M.

; Queiroz, Daniel de Medeiros

; Anselmo, Dory H.A.L.

Revista Brasileira de Ensino de Física

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2021, Volume 43 elocation e20210064

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Esse trabalho apresenta uma avaliação crítica multicontextual, histórica e conceitual, do Princípio de Pascal nos livros didáticos de Física aprovados no Programa Nacional do Livro Didático (PNLD) 2018. A análise realizada levou em consideração os seguintes aspectos: o enunciado do Princípio de Pascal sob o ponto de vista conceitual; relações entre ciência e tecnologia, tendo em vista dispositivos relacionados ao Princípio; fontes históricas primárias e secundárias relacionadas ao desenvolvimento do Princípio de Pascal; fundamentação historiográfica e apontamentos da legislação educacional para a inserção didática da História e da Filosofia da Ciência. Foram notadas inconsistências no que diz respeito à aplicabilidade do Princípio de Pascal, visões simplistas sobre a ciência e sobre as relações entre ciência e tecnologia, distorções históricas, anacronismos e abordagens historicamente descontextualizadas. Em face do referido cenário, são apontadas sugestões para uma inserção didática mais adequada.

This paper presents a multicontextual, historical and conceptual, critical analysis of Pascal’s Principle in Brazilian Physics textbooks from Programa Nacional do Livro Didático (PNLD) 2018. The analysis considered the following aspects: the statement of Pascal’s Principle from a conceptual perspective; relations between science and technology, in view of devices associated to the Principle; primary and secondary historical sources related to the development of Pascal’s Principle; historiographical foundation; and notes from Brazilian educational legislation concerned to didactic insertion of History and Philosophy of Science. We noticed inconsistencies regarding the applicability of Pascal’s Principle, simplistic views on science and on the relationship between science and technology, historical distortions, anachronisms, and historically decontextualized approaches. Suggestions are made considering this scenario, aiming at a more adequate didactic insertion.

https://doi.org/10.1590/1806-9126-rbef-2021-0064

Poor Sleep quality and health-related quality of life impact in adolescents with and without chronic immunosuppressive conditions during COVID-19 quarantine
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Helito, Alberto C.

; Lindoso, Livia

; Sieczkowska, Sofia M.

; Astley, Camilla

; Queiroz, Ligia B.

; Rose, Natalia

; Santos, Claudia Renata P.

; Bolzan, Thalis

; Peralta, Rita María I.A.

; Franco, Ruth R.

; Cominato, Louise

; Pereira, Rosa Maria R.

; Tannuri, Uenis

; Campos, Lucia Maria A.

; Lourenço, Benito

; Toma, Ricardo K.

; Medeiros, Karina

; Watanabe, Andréia

; Grangeiro, Patricia Moreno

; Farhat, Sylvia C.

; Casella, Caio B.

; Polanczyk, Guilherme V.

; Gualano, Bruno

; Silva, Clovis A.

; Sallum, Adriana M. E.

; Iraha, Amanda Y.

; Ihara, Bianca P.

; Mazzolani, Bruna C.

; Martinez, Claudia A.

; Strabelli, Claudia A. A.

; Fonseca, Claudia B.

; Lima, Dandara C. C.

; Setoue, Debora N. D.

; Roz, Deborah F. P.

; Smaira, Fabiana I.

; Roschel, Hamilton

; Miyatani, Helena T.

; Marques, Isabela G.

; Oba, Jane

; Ferreira, Juliana C. O.

; Simon, Juliana R.

; Kozu, Katia

; Saccani, Ligia P.

; Martiniano, Lorena V. M.

; Miranda, Luana C. A.

; Silva, Luiz E. V.

; Laurentino, Moisés F.

; Aikawa, Nadia E.

; Sakita, Neusa K.

; Tanigava, Nicolas Y.

; Pereira, Paulo R. A.

; Palmeira, Patrícia

; Angelo, Simone S.

; Lavorato, Sofia S. M.

; Bernardes, Tamires M.

; Franco, Tathiane C.

; Viana, Vivianne S. L.

; Barros, Vera P. M. F. R.

; Zheng, Yingying

Clinics

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2021, Volume 76 elocation e3501

Resumo: En | Texto: En | PDF: En

OBJECTIVE: To assess the possible factors that influence sleep quality in adolescents with and without chronic immunosuppressive conditions quarantined during the coronavirus disease 2019 (COVID-19) pandemic. METHODS: This cross-sectional study included 305 adolescents with chronic immunocompromised conditions and 82 healthy adolescents. Online surveys were completed, which included questions on socio-demographic data and self-rated healthcare routine during COVID-19 quarantine and the following validated questionnaires: the Pittsburgh Sleep Quality Index (PSQI), Pediatric Quality of Life Inventory 4.0 (PedsQL4.0), and Pediatric Outcome Data Collection Instrument (PODCI). RESULTS: The median current age [14 (10-18) vs. 15 (10-18) years, p=0.847] and frequency of female sex (62% vs. 58%, p=0.571) were similar in adolescents with chronic conditions compared with healthy adolescents. The frequency of poor sleep quality was similar in both groups (38% vs. 48%, p=0.118). Logistic regression analysis, including both healthy adolescents and adolescents with chronic conditions (n=387), demonstrated that self-reported increase in screen time (odds ratio [OR] 3.0; 95% confidence interval [CI] 1.3-6.8; p=0.008) and intrafamilial violence report (OR 2.1; 95% CI 1.2-3.5; p=0.008) were independently associated with poor sleep quality in these adolescents. However, the PODCI global function score was associated with a lower OR for poor sleep quality (OR 0.97; 95% CI 0.94-0.99; p=0.001). Further logistic regression, including only adolescents with chronic conditions (n=305), demonstrated that self-reported increase in screen time (OR 3.1; 95% CI 1.4-6.8; p=0.006) and intrafamilial violence report (OR 2.0; 95% CI 1.2-3.4; p=0.011) remained independently associated with poor quality of sleep, whereas a lower PODCI global function score was associated with a lower OR for sleep quality (OR 0.96; 95% CI 0.94-0.98; p<0.001). CONCLUSION: Self-reported increases in screen time and intrafamilial violence report impacted sleep quality in both healthy adolescents and those with chronic conditions. Decreased health-related quality of life was observed in adolescents with poor sleep quality.

https://doi.org/10.6061/clinics/2021/e3501

Unwrapping the long-term impacts of COVID-19 pandemic on Brazilian academic mothers: the urgency of short, medium, and long-term measures
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HIPÓLITO, JULIANA

; DIELE-VIEGAS, LUISA M.

; CORDEIRO, TÁBATA E.F.

; SALES, LILIAN P.

; MEDEIROS, ADRIANA

; DEEGAN, KATHLEEN R.

; LEITE, LUCIANA

Anais da Academia Brasileira de Ciências

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2020, Volume 92 Nº 4 elocation e20201292

Resumo: En | Texto: En | PDF: En

Abstract Since the beginning of the COVID-19 pandemic, publications have highlighted the disproportionate impact of the COVID-19 pandemic on academic mothers, mostly focusing on the impact of social distancing and quarantine. A few months later, despite the lack of effective vaccines or therapeutics in sight, many economic activities are being resumed. Nurseries and schools are expected to be among the latest to reopen, which will amplify the impacts of the pandemic on academic mothers. In this letter, we unwrap the pandemic impacts on academic mothers and describe a set of specific short-, medium- and long-term policies that, if implemented, could reduce setbacks for gender equality during the pandemic and can help to level the playing field for academic mothers.

https://doi.org/10.1590/0001-3765202020201292 1664 downloads

10.

Detection of Drug Resistant Mycobacterium Tuberculosis Strains Using Kit SIRE Nitratase®: a Multicenter Study
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Miranda, Silvana Spindola de

; Almeida, Isabela Neves de

; Mansur, Maria de Fátima Filardi Oliveira

; Figueredo, Lida Jouca de Assis

; Carvalho, Wânia da Silva

; Hadaad, João Paulo Amaral

; Diniz, Jaciara de Lourdes do Carmo Guimarães

; Groll, Andrea von

; Silva, Pedro Almeida da

; Lopes, Maria Luiza

; Santos, Marcelo Cordeiro dos

; Brito, Alexandra

; Mello, Fernanda Carvalho de Queiroz

; Malaquias, Thiago da Silva Santos

; Croda, Julio

; Pinhata, Juliana Maira Watanabe

; Oliveira, Rosângela Siqueira de

; Chimara, Erica

; Rossetti, Maria Lúcia

; Halon, Maria Laura

; Lourenço, Maria Cristina

; Medeiros, Reginalda Ferreira de Melo

; Montes, Fátima Cristina Onofre Fandinho

; Machado, Diana

; Viveiros, Miguel

; Kritski, Afrânio Lineu

Brazilian Archives of Biology and Technology

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2020, Volume 63 elocation e20190179

Resumo: En | Texto: En | PDF: En

Abstract (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.

https://doi.org/10.1590/1678-4324-2020190179 905 downloads

11.

Helicobacter spp. in domestic cats: identification and relationship with anatomical and histopathological gastric changes in animals of blood group A
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Sousa, Daniela A.

; Cascon, Clarice M.

; Mello, Marcela F.V.

; Leite, Juliana S.

; Medeiros, Miguel A.

; Fonseca, Ana Beatriz M.

; Ferreira, Ana Maria R.

Pesquisa Veterinária Brasileira

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Dez 2017, Volume 37 Nº 12 Páginas 1467 - 1473

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: O objetivo deste estudo foi avaliar a presença de organismos semelhantes a Helicobacter e as alterações endoscópicas e histopatológicas em estômago de gatos domésticos de sangue tipo A. Amostras de antro, corpo e fundo gástricos foram coletadas de 32 gatos, sem raça definida, não domiciliados através de gastroscopia. Teste de urease e análise citológica foram realizados em amostras frescas. Secções teciduais foram processadas e coradas com hematoxilina e eosina e pela prata pelo método de Warthin-Starry para avaliação histológica. Helicobacter spp. foi detectado em 100% das amostras submetidas às análises citológicas e coloração pela prata e em 96,9% das amostras submetidas ao teste de urease. Em 87,5% dos gatos foi identificado infiltrado inflamatório mononuclear. A graduação e distribuição do infiltrado inflamatório nestes gatos revelaram alterações leves (78,1%) a moderada (9,4%) em pelo menos uma região gástrica. Estas alterações eram independentes do escore de colonização. Hiperplasia de folículos linfoides foram detectadas em 3 gatos. Gatos do grupo sanguíneo A são frequentemente colonizados por Helicobacter spp. e os achados macro e microscópicos são consistentes com estudos em gatos domésticos realizados até a presente data. Conclui-se que o grupo sanguíneo mais comum em gatos não está associado com uma alta susceptibilidade a gastrite sintomática causada por Helicobacter spp.

ABSTRACT: The aim of this study was to evaluate the presence of gastric Helicobacter-like organisms and the endoscopic and histopathological changes in domestic cats with blood type A. Samples from the stomach antrum, body and fundus were collected from 32 mixed-breed stray domestic cats using gastroscopy. Urease testing and cytological analysis were performed in fresh samples. Tissue sections were processed and stained with hematoxylin and eosin (H&E) and the Warthin-Starry (WS) silver staining methods for histopathological examination. Helicobacter spp. were detected in 100% of samples subjected to silver staining and cytological analysis, and in 96.9% of samples subjected to urease testing. In 87.5% of the cats, mononuclear inflammatory-cell infiltrates were identified. The graduation and distribution of inflammatory infiltrates in these cats revealed mild (78.1%) to moderate (9.4%) inflammatory changes in at least one gastric region. These changes were independent of the colonization score. Hyperplasia of the lymphoid follicles was detected in three cats. Cats of blood group A are often colonized by Helicobacter spp. and the macroscopic and microscopic findings are consistent with studies in domestic cats reported to date, concluding that the most common blood group in cats is not associated with high susceptibility to symptomatic gastritis.

https://doi.org/10.1590/s0100-736x2017001200017 1447 downloads

12.

Larvicidal Activity of Beauveria bassiana Extracts against Aedes aegypti and Identification of Beauvericins
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Daniel, Juliana F. S.

; Silva, Andressa A.

; Nakagawa, Danielle H.

; Medeiros, Lívia S. de

; Carvalho, Mário G.

; Tavares, Lucineli J.

; Abreu, Lucas M.

; Rodrigues-Filho, Edson

Journal of the Brazilian Chemical Society

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Jun 2017, Volume 28 Nº 6 Páginas 1003 - 1013

Resumo: En | Texto: En | PDF: En

Beauveria bassiana is an entomopathogenic fungus that has been well known for its capacity to act as biopesticide on various disease vectors. The analysis of organic extracts of strains CG71 and UNI40 led to identification of cyclodepsipeptides beauvericin, beauvericin A or F, beauvericin E and bassianolide by ultra-high pressure liquid chromatography-high resolution mass spectrometry in tandem mode (UHPLC-HRMS/MS). The larvicidal activity on 3rd instar of Aedes aegypti revealed LC50 0.9887 and 0.4653 ppm in 24 and 48 hours (CG71 methanolic extract), LC50 0.7834 ppm in 48 hours (CG71 ethyl acetate), LC50 0.7834 and 1.8149 ppm (UNI 40 for ethyl acetate and methanolic extracts, respectively) in 48 hours. These findings highlight the potential of B. bassiana metabolites for controlling the vector of Dengue and Zika diseases.

https://doi.org/10.21577/0103-5053.20160253 1482 downloads

13.

DIFFERENT GENDER DIVISION PATTERNS FOR SWINE HOUSING IN WEAN-TO-FINISH SYSTEM
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Massari, Juliana M.

; Vercellino, Rimena do A.

; Curi, Thayla M. R. de C.

; Moura, Daniella J. de

; Medeiros, Brenda B. L.

; Salgado, Douglas D'Alessandro

Engenharia Agrícola

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Fev 2017, Volume 37 Nº 1 Páginas 13 - 23

Resumo: En | Texto: En | PDF: En

ABSTRACT This study aimed at identifying the best gender division pattern for swine at nursery stage, through the evaluation of behavioral and environmental parameters. Three treatments were established to achieve our objective: single-sex housing of 26 surgically castrated male pigs; mixed-sex housing of 13 castrated males and 13 females; and single-sex housing of 26 females. Environmental and behavioral data have been recorded. It was used an ethogram to make an inventory of behaviors and that was used in multivariate analysis. Bite addiction differed statistically among treatments, and the lowest occurrence was in mixed-sex housing. Results of the multivariate analysis suggested that regardless of the treatment chosen, relative humidity is positively associated with the production of gases and dry-bulb temperature with airspeed. Mixed-sex housing had multivariate relations suggesting less aggressive behaviors over food resources, being an example of social interaction that improves swine welfare.

https://doi.org/10.1590/1809-4430-eng.agric.v37n1p13-23/2017 1861 downloads

14.

Comparative study of five commercial probes for the detection of Epstein-Barr virus (EBV) by in situ hybridization in cases of nodular sclerosis Hodgki's lymphoma
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Nonogaki, Suely

; Shirata, Neuza K.

; Kimura, Lidia M.

; Guerra, Juliana M.

; Medeiros, Raphael S. S.

; Paes, Roberto Antonio P.

; Kanamura, Cristina T.

; Oliveira, Camila C.

; Menezes, Yara de

Jornal Brasileiro de Patologia e Medicina Laboratorial

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Dez 2016, Volume 52 Nº 6 Páginas 416 - 425

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Introdução: O vírus Epstein-Barr (EBV) pode servir como alvo nos tratamentos terapêuticos, sendo necessário resultado diagnóstico confiável. Objetivo: Avaliar a acurácia da detecção do EBV pela hibridização in situ (ISH), utilizando cinco sondas comerciais em amostras fixadas em formalina e incluídas em parafina de linfoma de Hodgkin (LH) esclerose nodular, comparando os resultados com a imuno-histoquímica (IHQ) e a reação em cadeia pela polimerase (PCR). Material e método: Trinta amostras foram selecionadas, sendo 28 linfonodos, uma medula óssea e um mediastino. Os seguintes parâmetros foram analisados: intensidade do sinal; proporcionalidade das células positivas; qualidade da reação de acordo com o conforto na avaliação, qualidade do sinal e homogeneidade das células marcadas; reação de fundo; morfologia; presença de artefatos; e positividade em outras células não neoplásicas. Todas as amostras foram analisadas para a detecção do EBV usando as cinco sondas, IHQ para proteína da membrana latente tipo 1 (LMP1) e PCR para antígeno nuclear do EBV (EBNA1). As análises estatísticas foram realizadas com o software R1; os índices de 5% para Kappa de Fleiss e Cohen foram considerados significantes. Resultados: A detecção pela IHQ-LMP1 foi de 26,7% (8/30) e 66,7% (20/30) pela PCR-EBNA1. Todas as sondas detectaram EBV. A positividade foi observada em 42/90 (46,7%), 38/90 (42,2%), 45/90 (50%), 27/90 (30%) e 61/90 (67,8%) para as sondas A, B, C, D e E, respectivamente. Discussão: Todas as sondas demonstraram positividade. Conclusão: A sonda E mostrou melhor taxa (67,8%), sensibilidade, especificidade e precisão (100%), boa correlação entre os diferentes observadores e com a PCR, além de ótimo custo/benefício.

ABSTRACT Introduction: Epstein-Barr virus (EBV) may serve as a target in therapeutic treatments, thus reliable diagnostic results are necessary. Objective: The aim of this study was to evaluate the accuracy of EBV detection by in situ hybridization (ISH) using five commercial probes in formalin-fixed and paraffin-embedded samples of nodular sclerosis Hodgkin's lymphoma (HL), and to compare the results with immunohistochemistry (IHC) and polymerase chain reaction (PCR). Material and method: Thirty samples were selected, 28 were lymph nodes, one bone marrow and one mediastinum. The following parameters were analyzed: signal intensity; proportionality of positive cells; quality of the reaction according to comfort for evaluation, sign quality and homogeneity of labeled cells; background reaction; morphology; presence of artifacts; and positivity in other non-neoplastic cells. All samples were analyzed for EBV detection using the five probes, IHC for latent membrane protein type 1 (LMP1) and PCR for Epstein Barr virus nuclear antigen 1 (EBNA1). Statistical analyses were performed with the R1 software; Fleiss' test and Cohen Kappa index of 5% were considered significant. Results: The detection by IHC-LMP1 was 26.7% (8/30) and 66.7% (20/30) by PCR-EBNA1. All probes detected EBV. Positivity was observed in 42/90 (46.7%), 38/90 (42.2%), 45/90 (50%), 27/90 (30%) and 61/90 (67.8%) for probes A, B, C, D and E, respectively. Discussion: All five probes demonstrated positivity. Conclusion: Probe E showed better rate (67.8%), sensitivity, specificity and accuracy (100%), a very good correlation among the different observers and with PCR, besides great cost-benefits relation.

https://doi.org/10.5935/1676-2444.20160065 2384 downloads

15.

Zoning of environmental conditions inside a wean-to-finish pig facility
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Massari, Juliana M.

; Moura, Daniella J. de

; Curi, Thayla M. R. de C.

; Vercellino, Rimena do A.

; Medeiros, Brenda B. L.

Engenharia Agrícola

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Out 2016, Volume 36 Nº 5 Páginas 739 - 748

Resumo: En | Texto: En | PDF: En

ABSTRACT Using geostatistics, this study aimed to analyze environmental conditions for wean-to-finish swines at 3 pm, during the most sensitive phases to thermal stress (growth/ finishing and finishing). To this end, dry-bulb temperature, relative humidity, air speed, ammonia and carbon dioxide concentrations were measured at 81 equidistant points within the barn. Descriptive statistics was used to classify attribute variability, generating mean, median, variation coefficient, asymmetry and kurtosis, through the normality of Kolmogorov-Smirnov (KS). For that, it was used Minitab 15 software. Moreover, semivariograms were used to assess spatial dependence using the GS+ software, and through Surfer, spatial distribution maps were designed. Dry-bulb temperature and relative humidity showed a more homogeneous distribution whether compared to the other attributes. Furthermore, critical points were observed within the facility, with temperatures and low humidity, indicating the need for fogging equipment and fans to improve thermal comfort and animal welfare.

https://doi.org/10.1590/1809-4430-Eng.Agric.v36n5p739-748/2016 1141 downloads

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