Abstract Objectives This study aimed to investigate the osseointegration of titanium (Ti) implants with micro-nano textured surfaces functionalized with strontium additions (Sr) in a pre-clinical rat tibia model. Methodology Ti commercially pure (cp-Ti) implants were installed bilaterally in the tibia of 64 Holtzman rats, divided into four experimental groups (n=16/group): (1) Machined surface - control (C); (2) Micro-nano textured surface treatment (MN); (3) Micro-nano textured surface with Sr2+ addition (MNSr); and (4) Micro-nano textured surface with a higher complementary addition of Sr2+ (MNSr+). In total, two experimental euthanasia periods were assessed at 15 and 45 days (n=8/period). The tibia was subjected to micro-computed tomography (μ-CT), histomorphometry with the EXAKT system, removal torque (TR) testing, and gene expression analysis by PCR-Array of 84 osteogenic markers. Gene expression and protein production of bone markers were performed in an in vitro model with MC3T3-E1 cells. The surface characteristics of the implants were evaluated by scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), and laser scanning confocal microscopy. Results SEM, confocal, and EDS analyses demonstrated the formation of uniform micro-nano textured surfaces in the MN group and Sr addition in the MNSr and MNSr+ groups. TR test indicated greater osseointegration in the 45-day period for treated surfaces. Histological analysis highlighted the benefits of the treatments, especially in cortical bone, in which an increase in bone-implant contact was found in groups MN (15 days) and MNSr (45 days) compared to the control group. Gene expression analysis of osteogenic activity markers showed modulation of various osteogenesis-related genes. According to the in vitro model, RT-qPCR and ELISA demonstrated that the treatments favored gene expression and production of osteoblastic differentiation markers. Conclusions Micro-nano textured surface and Sr addition can effectively improve and accelerate implant osseointegration and is, therefore, an attractive approach to modifying titanium implant surfaces with significant potential in clinical practice. (Ti micronano micro nano (Sr preclinical pre cpTi cp (cp-Ti 6 rats n=16/group n16group ngroup n 16 (n=16/group) 1 (1 C (C) 2 (2 Micronano Micro (MN) 3 (3 Sr2 (MNSr) 4 (4 MNSr+. . (MNSr+) total n=8/period. n8period nperiod n=8/period 8 (n=8/period) microcomputed computed μCT, μCT μ CT , (μ-CT) system (TR testing PCRArray PCR Array MC3T3E1 MCTE MC3T3 E1 MC T E MC3T3-E cells SEM (SEM) energydispersive energy dispersive EDS, (EDS) 45day day boneimplant osteogenesisrelated osteogenesis related genes RTqPCR RT qPCR is therefore practice (n=16/group ( (C (MN (MNSr (MNSr+ (n=8/period (μ-CT MC3T3E MCT MC3T (SEM (EDS

Resumo Introdução Toda má oclusão apresenta origem multifatorial, e dentre os fatores que a desencadeiam estão os hábitos. O surgimento de um hábito pode estar relacionado com o emocional, o fisiológico ou com o aprendizado condicionado do indivíduo. Objetivo Analisar as relações entre hábitos e más oclusões associado a problemas respiratórios em escolares de 5 anos. Material e método Foi realizado um estudo transversal com uma amostra de 753 crianças pertencentes à rede municipal de ensino da cidade de Araraquara, SP. Foram realizados exames clínicos para avaliação da má oclusão (Foster & Hamilton), além de aplicado questionários aos pais para avaliação de condição socioeconômica e demográfica, qualidade de vida relacionada à saúde bucal (ECOHIS) e hábitos. A análise dos dados foi feita pela análise bivariada por teste qui-quadrado seguida de modelo de regressão logística múltipla considerando nível de significância de 5%. Resultado A prevalência de crianças com problemas respiratórios e/ou alergias foi de 46,9%, com má oclusão foi de 58,7% e com algum tipo de hábito oral deletério foi de 12,0%. Apresentaram mais chance de ter problemas respiratórios e/ou alergias crianças do sexo masculino (1,40 vezes mais) e crianças com algum hábito oral deletério (1,95 vezes mais). Conclusão Observou-se que apresentaram maior chance de problemas respiratórios e/ou alergias as crianças do sexo masculino, crianças com algum hábito oral deletério e que apresentavam bruxismo.

Abstract Introduction All malocclusion has a multifactorial origin, and among the factors that trigger it are the habits. The appearance of a habit can be related to the emotional, physiological or conditioned learning of the individual. Objective Evaluate the relationship between habits and malocclusions associated with respiratory problems in 5-year-old students. Material and method A cross-sectional study were carried out with a sample of 753 children belonging to the municipal education network of the city of Araraquara-SP. Clinical exams were performed for malocclusion evaluation (Foster & Hamilton), applying questionnaires to the parents for evaluation of socioeconomic and demographic condition, oral health related quality of life (ECOHIS) and habits. Data analysis was performed by bivariate analysis using chi-square test followed by multiple logistic regression model considering 5% significance level. Result The prevalence of children with respiratory problems and/or allergies was 46.9%, with malocclusion was 58.7% and with some type of deleterious oral habit was 12.0%. Male children were 1.40 times more likely to present respiratory problems or allergies and children with some deleterious oral habits were 1.95 times more likely to present respiratory problems or allergies. Conclusion It was observed that male children, children that presented deleterious oral habits, and children with bruxism are more likely to have respiratory problems and/or allergies.

Abstract When periodontal disease is diagnosed, it is difficult to predict the clinical response of treatment of a tooth over time because the result of treatment is affected by several factors and will depend on the maintenance and support of periodontal treatment. Rehabilitation with removable dental prostheses, fixed prostheses, and dental implants makes it possible to restore the function and esthetics of patients with tooth loss due to periodontal disease. The predictive factors of tooth loss in periodontitis patients should be assessed by dentists to inform their clinical decision-making during dental treatment planning. This will provide detailed individualized information and level of risk of patients considered suitable for dental rehabilitation. Therefore, the aim of this article was to review the subject of “Impact of tooth loss due to periodontal disease on the prognosis of rehabilitation” and the effect of fixed, removable, and implant-supported prostheses in periodontal patients.

Resumo Introdução Problemas de origem dentária podem ocasionar um forte impacto em aspectos funcionais, emocionais e sociais das crianças, comprometendo-as em âmbito escolar, na aprendizagem e no seu desenvolvimento fisiológico. Objetivo Analisar as associações entre as variáveis de condições socioeconômicas, saúde bucal, hábitos e má oclusão com o desempenho escolar das crianças de cinco anos. Material e método A amostra consistiu em 753 crianças matriculadas em escolas públicas da cidade de Araraquara-SP. Foram realizados exames bucais para avaliar a presença de cárie dentária e má oclusão. Foram analisados, também, hábitos de sucção e bruxismo, condição socioeconômica e a qualidade de vida relacionada à saúde bucal. Os dados foram avaliados pelas análises descritivas e por modelos de regressão logística, simples e múltiplo (p≤0,05). Foram estimados os Odds Ratios, com intervalos de 95% de confiança. Resultado Apresentaram chances de ter pior desempenho escolar: crianças de famílias com menores rendas (1,76 vez), crianças que apresentaram piores condições de saúde bucal (1,93 vez), crianças que apresentaram um comportamento deficiente em relação à higiene oral (6,6 vezes) e crianças do sexo masculino (1,48 vez). Não houve evidências de associação entre as variáveis de saúde bucal, má oclusão e de hábitos orais deletérios com o desempenho escolar percebido pelos responsáveis. Conclusão Crianças de famílias com menores rendas, que recebem ajuda financeira do governo, com pior avaliação da saúde bucal e higiene oral, do sexo masculino e que são cuidadas por pessoas que não os pais apresentaram maior chance de ter o desempenho escolar comprometido.

Abstract Introduction Problems of dental origin can cause a strong impact on functional, emotional and social aspects of children, compromising them at school, in learning and in their physiological development. Objective Evaluate the associations between variables of socioeconomic conditions, oral health, habits and malocclusion with the school performance of 5-year-old children. Material and method The sample consisted of 753 children enrolled in public schools in the city of Araraquara-SP. Oral examinations were performed to evaluate the presence of dental caries and malocclusion. We also analyzed sucking habits, bruxism, socioeconomic status and quality of life related to oral health. Data were evaluated by descriptive analyses and logistic regression models, simple and multiple (p≤0.05). Odds Ratios (OR) were estimated, with 95% confidence intervals. Result Children from families with lower income (1.76 times), children with worse oral health conditions (1.93 times), children with poor oral hygiene behavior (6.6 times), and male children (1.48 times) were more likely to have worse school performance. There was no evidence of association between the variables of oral health, malocclusion and deleterious oral habits with the school performance perceived by the guardians. Conclusion Children from families with lower incomes, who receive financial help from the government, with worse evaluation of oral health and oral hygiene, males and who are cared for by people other than their parents were more likely to have their school performance compromised.

Resumo Introdução A quantidade e qualidade óssea na implantodontia é um fator de alta relevância quando se tem por objetivo instalar implantes e reabilitar pacientes. No entanto, essa disponibilidade é comprometida na maioria dos casos, havendo a necessidade da busca de novos biomateriais, membranas e substâncias para uma regeneração mais favorável. Objetivo: O objetivo deste estudo foi avaliar a resposta da neoformação óssea em defeitos críticos em calvárias de ratos utilizando scaffolds de fibras de blenda polimérica a partir de poli (ácido láctico-co-glicólico) e poli-isopreno (Cellprene®). O projeto foi aprovado pelo Comitê de Ética em Experimentação Animal. Material e método Neste estudo, foram utilizados 36 ratos (Rattus Norvegicus), variação albinus, Holtzman, adultos. Os animais foram submetidos à tricotomia na região da calota craniana e à confecção de defeitos ósseos circulares bilaterais com 5 mm de diâmetro. Os animais foram divididos em três grupos: GC – defeito sem colocação de biomaterial; GCol – scaffolds de colágeno (Bio-Gide, da empresa Geistlich Pharma Ag – Biomaterials); GPoli – scaffolds de fibras de blenda polimérica a partir de poli (ácido láctico-co-glicólico - Cellprene®). Cada grupo foi avaliado em quatro períodos experimentais (7, 15, 30 e 60 dias). Após esses períodos, os animais foram sacrificados, e as peças passaram por tramitação laboratorial de rotina e inclusão em parafina. Foram obtidos cortes semisseriados e corados pela técnica de hematoxilina e eosina para análise histométrica e histológica. Foi executada análise histométrica para avaliar a composição do tecido ósseo reparado (% osso). Os dados obtidos foram analisados estatisticamente com nível de significância de 95%. Resultado Foi verificado que o GCol apresentou maior preenchimento do defeito nos períodos de 30 e 60 dias em comparação aos GC e GPoli. Conclusão Os scaffolds de fibras de blenda polimérica a partir de poli (ácido láctico-co-glicólico) e poli-isopreno (Cellprene®) não apresentaram vantagens quando utilizados em defeitos críticos.

Abstract Introduction The bone quantity and quality in implant dentistry is a highly relevant factor when it aims the use of implants and rehabilitation in patients. However, this availability is compromised in most cases, with the need to research new biomaterials, membranes and substances for more favorable regeneration. Objective: The aim of this study was to evaluate the response of bone neoformation in critical defects in rat calvaries using polymeric blend fiber scaffolds from Poly (Lactic-Co-Glycolic Acid) and Polyisoprene (Cellprene®). The project was approved by the Animal Experimentation Ethics Committee. Material and method In this study 36 rats (Rattus Norvegicus), variation albinus, Holtzman, adults were used. The animals had trichotomy in the region of the skull and the confection of bilateral circular bone defects with a diameter of 5 mm. The animals were divided into 3 groups: Group GC - defect without biomaterial placement, Group GCol - collagen scaffolds (Bio-Gide, from Geistlich Pharma Ag - Biomaterials), Group GPoli - polymeric blend fiber scaffolds from Poly (Lactic-Co-Glycolic Acid)-Polyisoprene. Each group was evaluated in 4 experimental periods (7, 15, 30 and 60 days). After these periods the animals were sacrificed and the pieces underwent routine laboratory procedures and paraffin embedding. Semi-serial sections were obtained and stained by hematoxylin and eosin technique for histometric and histological analysis. Histometric analysis was performed to evaluate the composition of repaired bone tissue (% Bone). The data obtained were statistically analyzed with a significance level of 95%. Result It was found that the GCol group presented greater defect filling in the 30 and 60 days periods compared to the GC and GPoli groups. Conclusion Polymer blend fiber scaffolds from Poly (Lactic-Co-Glycolic Acid) and Polyisoprene (Cellprene®) did not have advantages when used in critical defects.