Pesquisa | SciELO

ABSTRACT Objective: To report outcomes from the largest multicenter series of penile cancer patients undergoing video endoscopic inguinal lymphadenectomy (VEIL). Materials and Methods: Retrospective multicenter analysis. Authors of 21 centers from the Penile Cancer Collaborative Coalition-Latin America (PeC-LA) were included. All centers performed the procedure following the same previously described standardized technique. Inclusion criteria included penile cancer patients with no palpable lymph nodes and intermediate/high-risk disease and those with non-fixed palpable lymph nodes less than 4 cm in diameter. Categorical variables are shown as percentages and frequencies whereas continuous variables as mean and range. Results: From 2006 to 2020, 210 VEIL procedures were performed in 105 patients. Mean age was 58 (45-68) years old. Mean operative time was 90 minutes (60-120). Mean lymph node yield was 10 nodes (6-16). Complication rate was 15.7%, including severe complications in 1.9% of procedures. Lymphatic and skin complications were noted in 8.6 and 4.8% of patients, respectively. Histopathological analysis revealed lymph node involvement in 26.7% of patients with non-palpable nodes. Inguinal recurrence was observed in 2.8% of patients. 10y- overall survival was 74.2% and 10-y cancer specific survival was 84.8%. CSS for pN0, pN1, pN2 and pN3 were 100%, 82.4%, 72.7% and 9.1%, respectively. Conclusion: VEIL seems to offer appropriate long term oncological control with minimal morbidity. In the absence of non-invasive stratification measures such as dynamic sentinel node biopsy, VEIL emerged as the alternative for the management of non-bulky lymph nodes in penile cancer.

https://doi.org/10.1590/s1677-5538.ibju.2023.0065

Impact of monochromatic lights on the in vitro development of Cattleya walkeriana and effects on acclimatization
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Nadal, Michele Carla

; Machado, Natalia Bernardes

; Santos, Cyntia Stephânia dos

; Flores, José Henrique Nunes

; Dória, Joyce

; Pasqual, Moacir

Ornamental Horticulture

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2023, Volume 29 Nº 2 Páginas 238 - 248

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Light quality is an important factor for the adequacy of plant production through plant tissue culture, as it directly interferes with morphogenesis and photosynthetic capacity of explants. The objective of this study was to analyze the impact of monochromatic lights such as light emitting diode (LED) on the in vitro development of Cattleya walkeriana G. and their effects on acclimatization. The plants were developed in vitro under the colors of green, blue, yellow, red, 2 red:1 blue, and white LED lights. For in vitro cultivation, Knudson medium was used, supplemented with 20 mg L-1 sucrose, 5.5 mg L-1 agar, 2% activated charcoal, 100 mL coconut water, and pH 6.0. For ex vitro cultivation, the plants were acclimatized in styrofoam trays containing sphagnum as a substrate. In general, the supplied light lengths impactedin vitro growth and acclimatization analyses. There was influence on the cuticle thickness of plants in vitro. Chlorophyll and carotenoid contents were not significant. We can conclude that light lengths formed by 2 red:1 blue, red and yellow LEDs can be indicated for better performance in the production of C. walkeriana. The 2 red:1 blue and red LEDs provide superior in vitro development than the others, with gains for the species in acclimatization. The yellow LED provided a possible in vitro hardening, which ensured the greatest success of the seedlings during acclimatization.

Resumo A qualidade luminosa é um fator importante para a adequação da produção de plantas através da cultura de tecidos vegetais, pois interfere diretamente nos processos de morfogênese e na capacidade fotossintéticas dos explantes. O objetivo deste estudo foi analisar o impacto de luzes monocromática do tipo diodo emissor de luz (light-emittingdiode - LED) no desenvolvimento in vitro de Cattleya walkeriana G. e os efeitos na fase aclimatização. As plantas foram desenvolvidas in vitro sob as cores de luzes do tipo LED verde, azul, amarelo, vermelho, 2 vermelhos: 1 azul, e branco. Para cultivo in vitro foi utilizado o meio Knudson acrescido de 20 mg L-1 de sacarose, 5,5 mg L-1 de ágar, 2% de carvão ativado, 100 mL de água de coco, e pH 6,0. Para o cultivo ex vitro as plantas foram aclimatizadas em bandejas de isopor contendo esfagno como substrato. De maneira geral, os comprimentos luminosos fornecidos apresentaram impactos nas análises de crescimento in vitro e na de aclimatização. Houve influência na espessura da cutícula das plantas in vitro. Os teores de clorofilas e carotenoides não foram significativos. Podemos concluir, que comprimentos luminosos formados pelos LEDs 2 vermelhos: 1 azul, vermelho e amarelo podem ser indicados para melhor desempenho na produção de C. walkeriana. Os LEDs 2 vermelhos: 1 azul, e o LED vermelho propiciam desenvolvimento in vitro superior ao demais com ganhos na fase de aclimatização para espécie. O LED amarelo propiciou um possível processo de rustificação in vitro o que garantiu o maior sucesso das mudas no período de aclimatização.

https://doi.org/10.1590/2447-536x.v29i2.2610

Synthesis and Anticancer Activity of Homodimeric Morita-Baylis-Hillman Adducts Based on 3-Hydroxyindolin-2-one Core
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Coelho, Maísa C.

; Castro, Aleff

; Olegário, Tayná R.

; Cristiano, Rodrigo

; Vaz, Boniek G.

; Santos, Gabriel F. dos

; Machado, Lucas S.

; Militão, Gardênia C. G.

; Silva, Paulo B. N. da

; Vasconcellos, Mário L. A. A.

; Lima-Junior, Claudio G.

Journal of the Brazilian Chemical Society

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2023, Volume 34 Nº 9 Páginas 1273 - 1283

Resumo: En | Texto: En | PDF: En

Cancer treatment represents one of the main scientific study targets today, mainly due to the pronounced side effects arising from chemotherapy. This study reports the synthesis, characterization, and anticancer activity of ten compounds from the Morita-Baylis-Hillman reaction. Ethylene glycol diacrylate was used as a double Michael acceptor in reactions with isatin derivatives to give homodimers of 3-hydroxyindolin-2-one core, recognized in the literature for its extensive pharmacological profile. The use of 1,4-diazabicyclo[2,2,2]octane (DABCO) as a catalyst and room temperature were the optimal conditions for the study reaction. The isolated yields were up to 63%, with most reaction times inferior to 24 h, some as fast as 15 min. The anticancer potential of the synthesized dimers was evaluated in vitro against three cancer strains, resulting in average inhibitory concentrations up to 0.72 µM. It was also found that the best performing homodimers are more active than their monomeric counterparts. Considering the promising selectivity indices observed, the preliminary results obtained here act as a basis for broader tests regarding the effectiveness of homodimeric adducts against cancer cells.

https://doi.org/10.21577/0103-5053.20230038

Medical research: Are e-Sports really sports? research eSports e Sports sports
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Scorza, Fulvio A.

; Fiorini, Ana C.

; Lima, Camila C. de

; Camilo, Nilton

; Magro, Eliana P.

; Guimarães, Liliane

; Pavanelli, Claudio

; Finsterer, Josef

; Almeida, Antonio-Carlos G. de

; Fonseca, Marcelo C.M.

; Fujita, Reginado R.

; Barros, Turibio L. de

; Scorza, Miguel M.C.

; Moret, Marcelo A.

; Teixeira-Machado, Lavínia

; Arida, Ricardo M.

Clinics

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2023, Volume 78 elocation 100190

Resumo: En | Texto: En | PDF: En

https://doi.org/10.1016/j.clinsp.2023.100190

Commercial characteristics of coffee seedlings produced with different sources of phosphorus and plant growth-promoting bacteria growthpromoting growth promoting
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Ferraro, A. C.

; França, A. C.

; Machado, C. M. M.

; Aguiar, F. R.

; Oliveira, L. L.

; Braga Neto, A. M

; Oliveira, R. G.

Brazilian Journal of Biology

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2023, Volume 83 elocation e270262

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Considerado o motor econômico de muitos países, a cultura do café representa um importante componente da cadeia agrícola no Brasil. Os crescentes valores de comercialização, das áreas de plantio e da produtividade da cultura requerem aquisição de mudas de qualidade, que devem receber adequado aporte nutricional através do uso eficiente de fertilizantes. Fertilizantes de liberação lenta e controlada, a exemplo dos organominerais, ganham destaque quando se trata de aumento da eficiência no uso do fósforo, assim como as bactérias promotoras de crescimento de plantas (BPCP) com características solubilizadoras de fosfato. Esse estudo teve como objetivo avaliar o efeito de diferentes fontes de fertilizantes mineral e organomineral, inoculados e não inoculados com BPCP, sobre parâmetros de qualidade de mudas de café. De forma geral, as fontes de P utilizadas no experimento interferiram positivamente no desenvolvimento das mudas de café. Isso comprova que existe a necessidade de complementação nutricional para um bom desenvolvimento das mudas. Dentre as fontes utilizadas, o organomineral na forma granulada apresentou melhor desempenho nos parâmetros de crescimento e fisiológicos das mudas de café, mostrando ser uma alternativa viável aos fertilizantes comumente utilizados. A adição de BPCP apresentou vantagem significativa para as variáveis de qualidade das mudas. países Brasil comercialização controlada organominerais fósforo (BPCP fosfato geral utilizados

Abstract Considered the economic engine of many countries, the coffee culture represents an important component of the agricultural chain in Brazil. The growing values of commercialization, planting areas, and crop productivity require the acquisition of quality seedlings, which must receive adequate nutritional support through efficient fertilizers. Slow and controlled-release fertilizers, such as organominerals, gain prominence when it comes to increasing efficiency in the use of phosphorus, as well as plant growth-promoting bacteria (PGPB) with phosphate solubilizing characteristics. This study aimed to evaluate the effect of different sources of mineral and organomineral fertilizers, inoculated and non-inoculated with PGPB on the quality parameters of coffee seedlings. In general, the P sources used in the experiment positively interfered with the development of coffee seedlings. This proves that there is a need for nutritional supplementation for the good development of the seedlings. Among the sources used, the organomineral in granulated form showed better performance in coffee seedlings' growth and physiological parameters, proving to be a viable alternative to commonly used fertilizers. The addition of PGPB showed a significant advantage for seedling quality variables. countries Brazil commercialization areas seedlings fertilizers controlledrelease controlled release organominerals phosphorus growthpromoting promoting (PGPB characteristics noninoculated non general variables

https://doi.org/10.1590/1519-6984.270262

Biotechnological and socio-environmental potential of Campomanesia adamantium (Myrtaceae): an interdisciplinary review socioenvironmental socio environmental Myrtaceae (Myrtaceae) (Myrtaceae
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Zorgetto-Pinheiro, V. A.

; Kuff, H. M.

; Machado, G. T.

; Guimarães, R. C. A.

; Pott, A.

; Campos, R. P.

; Nascimento, V. A.

; Bogo, D.

Brazilian Journal of Biology

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2023, Volume 83 elocation e273473

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Guavira (Campomanesia adamantium, Myrtaceae) é um fruto nativo do Cerrado brasileiro e de importância sócio econômica para a população indígena e tradicional do Centro-Oeste. Esta é uma revisão bibliográfica das propriedades da guavira e seus derivados e, após a discussão de estudos experimentais, uma abordagem interdisciplinar é conduzida destacando a importância dos Sistemas Agroflorestais como uma ferramenta de restauração ecológica para alavancar a cadeia produtiva da guavira enquanto fornece serviços ecossistêmicos. Muitos grupos de pesquisa estudaram os efeitos dos polifenóis e outros compostos bioativos e propriedades biológicas desta fruta e de outras partes da planta, como antibióticos, antioxidantes, anti-inflamatórios, anti-hiperlipidêmicos, antidiarreicos e antitumorais, proteção cardiovascular e hepática e ação contra a dor neuropática. Além disso, os subprodutos da guavira beneficiam a saúde intestinal das aves, da mesma forma que os antibióticos adicionados à sua alimentação. Foram, também, encontrados diversos produtos biotecnológicos, como farinha de polpa, tempero da casca, protetor solar e óleo de semente semelhante ao azeite com potencial farmacêutico e industrial. Concluímos enfatizando a importância da guavira para a restauração e preservação do ameaçado Cerrado brasileiro e para o desenvolvimento socioambiental da agricultura familiar. A mesma abordagem e estudo são bem-vindos e necessários em outras regiões e domínios em todo o mundo, tendo sua flora nativa como meio para um fim restaurador. Campomanesia adamantium Myrtaceae CentroOeste. CentroOeste Centro Oeste. Oeste Centro-Oeste experimentais ecossistêmicos planta antioxidantes antiinflamatórios, antiinflamatórios anti inflamatórios, inflamatórios anti-inflamatórios antihiperlipidêmicos, antihiperlipidêmicos hiperlipidêmicos, hiperlipidêmicos anti-hiperlipidêmicos antitumorais neuropática disso aves alimentação Foram também biotecnológicos polpa casca industrial familiar bemvindos bem vindos mundo restaurador

Abstract Guavira (Campomanesia adamantium, Myrtaceae) is a native fruit from the Brazilian Cerrado savanna and is socio-economically important for the indigenous and traditional people living in the Central-West. This is a bibliographic review of the biological properties of guavira and its derivatives, and, after discussing experimental studies, an interdisciplinary approach is conducted highlighting the im-portance of Agroforestry Systems as an ecological restoration tool to leverage the production chain of guavira while providing ecosystem services. Many research groups studied effects of polyphenols and other bioactive compounds and biological properties of this fruit and other plant parts such as antibiotic, antioxidant, anti-inflammatory, anti-hyperlipidemic, anti-diarrheic and antitumoral activities, cardiovascular and hepatic protection and action against neuropathic pain. Besides, guavira by-products benefit poultry intestinal health, similarly to antibiotics added to their feed. Furthermore, several biotechnological products were found, like pulp flour, seasoning from the peel, sunscreen, and seed oil similar to olive oil with pharmaceutical and industrial potential. We conclude by emphasizing the importance of guavira for restoration and preservation of the threatened Brazilian Cerrado, and for the socio-environmental development of family agriculture. The same approach and study are welcome and necessary in other regions and domains worldwide having their native flora as means for a restorative end. Campomanesia adamantium Myrtaceae socioeconomically socio economically CentralWest. CentralWest Central West. West Central-West derivatives studies im portance services antibiotic antioxidant antiinflammatory, antiinflammatory anti inflammatory, inflammatory anti-inflammatory antihyperlipidemic, antihyperlipidemic hyperlipidemic, hyperlipidemic anti-hyperlipidemic antidiarrheic diarrheic activities pain Besides byproducts health feed Furthermore found flour peel sunscreen potential socioenvironmental environmental agriculture end

https://doi.org/10.1590/1519-6984.273473

Poly(vinyl alcohol)-Based Electrospun Nanofibers with Improved Antimicrobial Activity
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Machado, Paola A. P.

; Fraga, Gabriel N.

; Medeiros, Alessandra R.

; Pellá, Michelly C. G.

; Colauto, Nelson B.

; Linde, Giani A.

; Cavalheiro, Fernando N.

; Faria, Maria G. I.

; Dragunski, Douglas C.

; Caetano, Josiane

Journal of the Brazilian Chemical Society

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2023, Volume 34 Nº 12 Páginas 1852 - 1862

Resumo: En | Texto: En | PDF: En

Electrospinning was used to produce poly(vinyl alcohol) (PVA)-based nanofibers containing nerolidol (NER) as the antimicrobial agent. The scanning electron microscopy results indicated the formation of homogeneous fibers in the absence of NER. However, its incorporation into the fiber prevented the complete solvent evaporation, ultimately leading to the coalescence of the fibers. It became more evident as the amount of NER increased from 0 to 50% m/v. The incorporation of NER also decreased the crystallinity of the fibers from 10 to ca. 6.5%. However, this decrease was not proportional to the concentration of NER. The contact angle results confirmed the presence of NER molecules on the surface of the fibers especially due to the increase of the contact angle from 0 (PVA/NER0) to 27.8º (PVA/NER50). The fibrous mats exhibited good antimicrobial activity, being even more efficient than the control in some cases, suggesting their potential for wound-dressing purposes.

https://doi.org/10.21577/0103-5053.20230078

Diversity of eukaryotic and prokaryotic microbiota revealed by metabarcoding in Neotropical floodplain lakes
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TARGUETA, CÍNTIA P.

; ANTUNES, ADRIANA M.

; MACHADO, KARINE B.

; FERNANDES, JORDANA G.

; TELLES, MARIANA P. DE C.

; VIEIRA, LUDGERO C. GALLI

; LOGARES, RAMIRO

; NABOUT, JOÃO C.

; SOARES, THANNYA N.

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Supl. 1 elocation e20201578

Resumo: En | Texto: En | PDF: En

Abstract The diversity of eukaryotic and prokaryotic communities has been assessed by morphological and genetic approaches, which are used to characterize the microbiota in different environments. Here, planktonic prokaryotic and eukaryotic communities of the Araguaia River, located in the Central region of Brazil, were analyzed based on metabarcoding analysis of rRNA genes to evaluate the diversity of these groups in tropical floodplain lakes. Also, we tested their spatial concordance throughout the Araguaia river. Water samples were collected from 8 floodplain lakes in Araguaia River. The 16S and 18S rRNA genes were amplified and sequenced using Illumina MiSeq. For eukaryotes, 34,242 merged reads were obtained and 225 distinct OTUs were delineated, of which 106 OTUs were taxonomically classified. For prokaryotes, 26,426 sequences were obtained and 351 OTUs were detected. Of them, 231 were classified in at least one taxonomic category. The most representative eukaryotes belonged to Ciliophora, Chlorophyta and Charophyta. The prokaryotic phylum with the most OTUs classified were Proteobacteria, Actinobacteria and Bacteroidetes. The lakes did not show spatial concordance when comparing the similarity between their microbiota. The knowledge of freshwater biodiversity using DNA sequencing for important rivers, such as Araguaia River, can improve microbiota inventories of tropical biodiversity hotspots.

https://doi.org/10.1590/0001-3765202320201578

Eugenia uniflora: a promising natural alternative against multidrug-resistant bacteria
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Ferreira, M. R. A.

; Lima, L. B.

; Santos, E. C. F.

; Machado, J. C. B.

; Silva, W. A. V.

; Paiva, P. M. G.

; Napoleão, T. H.

; Soares, L. A. L.

Brazilian Journal of Biology

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2023, Volume 83 elocation e274084

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract This work aimed to evaluate the chemical composition, antioxidant and antimicrobial activities from crude extract and fractions from leaves of Eugenia uniflora Linn. The crude extract was obtained by turbo extraction and their fractions by partitioning. Chromatographic analysis were performed, and the antioxidant capacity was verified by two methods (DPPH• and ABTS•+). The Minimal Inhibitory/Bactericidal Concentration were conducted against twenty-two bacteria, selecting five strains susceptible to extract/fractions and resistant to the antibiotics tested. Ampicillin, azithromycin, ciprofloxacin, and gentamicin were associated with Ethyl Acetate Fraction (EAF) against multidrug-resistant strains in modulatory and checkerboard tests. The chromatographic data showed gallic acid, ellagic acid, and myricitrin in crude extract, with enrichment in the EAF. The electron transfer activity demonstrated in the antioxidant tests is related to the presence of flavonoids. The Gram-positive strains were more susceptible to EAF, and their action spectra were improved by association, comprising Gram-negative bacilli. Synergisms were observed to ciprofloxacin and gentamicin against Pseudomonas aeruginosa colistin-resistant. The results demonstrate that the extract and enriched fraction obtained from the leaves of E. uniflora act as a promising natural alternative against multidrug-resistant bacteria.

Resumo Este trabalho teve como objetivo avaliar a composição química, as atividades antioxidantes e antimicrobianas do extrato bruto e frações de folhas de Eugenia uniflora Linn. O extrato bruto foi obtido por turbólise e suas frações por partição. Foram realizadas análises cromatográficas e a capacidade antioxidante foi verificada por dois métodos (DPPH• e ABTS•+). A Concentração Inibitória/Bactericida Mínima foi realizada contra vinte e duas bactérias, selecionando cinco cepas suscetíveis a extração/frações e resistentes aos antibióticos testados. Ampicilina, azitromicina, ciprofloxacina e gentamicina foram associados à Fração Acetato de Etila (FAE) contra cepas multirresistentes em testes modulatórios e de checkboard. Os dados cromatográficos mostraram ácido gálico, ácido elágico e miricitrina em extrato bruto, com enriquecimento na FAE. A atividade de transferência de elétrons demonstrada nos testes antioxidantes está relacionada com a presença de flavonoides. As cepas de Gram-positivas foram mais suscetíveis à FAE, e seus espectros de ação foram melhorados por associação, compreendendo bacilos Gram-negativos. Foram observados sinergismos de ciprofloxacina e gentamicina contra Pseudomonas aeruginosa resistente à colistina. Os resultados demonstram que o extrato e a fração enriquecida obtida das folhas de E. uniflora atuam como uma alternativa natural promissora contra bactérias multirresistentes.

https://doi.org/10.1590/1519-6984.274084

10.

16S metabarcoding analysis reveals the influence of organic and conventional farming practices on bacterial communities from the rhizospheric of Coffea arabica L.
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Andrade, P. H. M.

; Machado, P. C.

; Paula, A. F.

; Paganin, A. C. L.

; Rezende, G. S.

; Matheucci Jr., E.

; Carvalho, L. M.

; Freire, C. C. M.

; Cunha, A. F.

; Lacava, P. T.

Brazilian Journal of Biology

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2023, Volume 83 elocation e274070

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Coffea sp. is cultivated in many tropical countries. Brazil has always adopted intensive agricultural practices, but organic coffee farming is an alternative system based on the non-use of agrochemicals and the rational management of soils. Metabarcoding 16S analysis using next-generation sequencing has been developed to identify and compare the diversity of the Coffea arabica L. rhizospheric bacterial community in two farming areas in São Paulo, Brazil. Dourado uses conventional farming, while Ribeirão Corrente uses organic. We found broad taxonomic composition, with sequences from 24 phyla, 55 classes, 61 orders, 146 families, and 337genus. The three most abundant phyla were Proteobacteria (38.27%), Actinobacteria (15.56%), and Acidobacteria (16.10%). In organic farming, the top 3 were the family Sphingomonadaceae, order Rhizobiales, genus Nocardioides, and Gp6. The genus Gp2 and the phylum Candidatus Saccharibacteria were the most abundant OTUs exclusively present in conventional farming. In the organic farming practice, Proteobacteria, Actinobacteria, and Acidobacteria were also present among the exclusive OTUs; we also found OTUs belonging to Bacteroidetes, Firmicutes, and Verrucomicrobia. Our study indicates a positive effect of organic farming on microbial communities. Fertilization may directly affect soil microbiota, suggesting that a large and active microbial community low in functional diversity might not adapt to new climatic conditions. A diverse community could provide better resilience to environmental changes, improving the productivity of this important crop.

Resumo Coffea sp. é cultivada em muitos países tropicais. O Brasil sempre adotou práticas agrícolas intensivas, mas a cafeicultura orgânica é um sistema alternativo baseado na não utilização de agrotóxicos e no manejo racional dos solos. A análise Metabarcode 16S utilizando o sequenciamento de última geração foi desenvolvida para identificar e comparar a diversidade da comunidade bacteriana rizosférica de Coffea arabica L. em duas áreas de cultivo em São Paulo, Brasil. Dourado usa agricultura convencional, enquanto Ribeirão Corrente usa agricultura orgânica. Encontramos ampla composição taxonômica, com sequências de 24 filos, 55 classes, 61 ordens, 146 famílias e 337 gêneros. Os três filos mais abundantes foram Proteobacteria (38,27%), Actinobacteria (15,56%) e Acidobacteria (16,10%). Na agricultura orgânica, os 3 primeiros foram a família Sphingomonadaceae, ordem Rhizobiales, gênero Nocardioides e Gp6. O gênero Gp2 e o filo Candidatus Saccaribacteria foram as OTUs mais abundantes exclusivamente presentes na agricultura convencional. Na prática da agricultura orgânica, Proteobacteria, Actinobacteria e Acidobacteria também estiveram presentes entre as OTUs exclusivas; também encontramos OTUs pertencentes a Bacteroidetes, Firmicutes e Verrucomicrobia. Nosso estudo indica um efeito positivo da agricultura orgânica nas comunidades microbianas. A fertilização pode afetar diretamente a microbiota do solo, sugerindo que uma grande e ativa comunidade microbiana com baixa diversidade funcional pode não se adaptar às novas condições climáticas. Uma comunidade microbiana diversificada poderia proporcionar maior resiliência às mudanças ambientais, melhorando a produtividade desta importante cultura agrícola.

https://doi.org/10.1590/1519-6984.274070

11.

Bioinformatics and expression analysis of the Xeroderma Pigmentosum complementation group C (XPC) of Trypanosoma evansi in Trypanosoma cruzi cells
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Souza, K. M.

; Mendes, I. C.

; Dall’Igna, D. M.

; Repolês, B. M.

; Resende, B. C.

; Moreira, R. S.

; Miletti, L. C.

; Machado, C. R.

; Vogel, C. I. G.

Brazilian Journal of Biology

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2023, Volume 83 elocation e243910

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo O reparo por excisão de nucleotídeos (NER) atua reparando danos no DNA, como lesões causadas por cisplatina. A proteína Xeroderma Pigmentosum complementation group C (XPC) está envolvida no reconhecimento de danos pela via de reparação global do genoma pelo NER (GG-NER) e tem sido estudada em diferentes organismos devido à sua importância em outros processos celulares. Neste trabalho, estudamos proteínas do NER em Trypanosoma cruzi e Trypanosoma evansi, parasitos de humanos e animais, respectivamente. Modelos tridimensionais das proteínas XPC de T. cruzi e T. evansi foram feitos e observou-se poucas diferenças estruturais entre estas proteínas. Durante testes, a inserção do gene XPC de T. evansi (TevXPC) em T. cruzi resultou em crescimento celular mais lento em condições normais. Após o tratamento com cisplatina, T. cruzi superexpressando seu próprio gene XPC (TcXPC) foi capaz de recuperar as taxas de divisão celular mais rapidamente do que T. cruzi expressando o gene TevXPC. Com base nesses testes, sugere-se que TevXPC (sendo uma proteína exógena em T. cruzi) interfere negativamente nos processos celulares em que TcXPC (a proteína endógena) está envolvida. Isso provavelmente ocorreu pois TevXPC é capaz de interagir com algumas moléculas ou proteínas endógenas de T.cruzi, mas é incapaz de interagir com outras. Isso reforça a importância do correto funcionamento de XPC dentro da célula.

Abstract Nucleotide excision repair (NER) acts repairing damages in DNA, such as lesions caused by cisplatin. Xeroderma Pigmentosum complementation group C (XPC) protein is involved in recognition of global genome DNA damages during NER (GG-NER) and it has been studied in different organisms due to its importance in other cellular processes. In this work, we studied NER proteins in Trypanosoma cruzi and Trypanosoma evansi, parasites of humans and animals respectively. We performed three-dimensional models of XPC proteins from T. cruzi and T. evansi and observed few structural differences between these proteins. In our tests, insertion of XPC gene from T. evansi (TevXPC) in T. cruzi resulted in slower cell growth under normal conditions. After cisplatin treatment, T. cruzi overexpressing its own XPC gene (TcXPC) was able to recover cell division rates faster than T. cruzi expressing TevXPC gene. Based on these tests, it is suggested that TevXPC (being an exogenous protein in T. cruzi) interferes negatively in cellular processes where TcXPC (the endogenous protein) is involved. This probably occurred due interaction of TevXPC with some endogenous molecules or proteins from T.cruzi but incapacity of interaction with others. This reinforces the importance of correctly XPC functioning within the cell.

https://doi.org/10.1590/1519-6984.243910 462 downloads

12.

Gastroesophageal tube of the Iguana iguana (Iguanidae): histological description, histochemical and immunohistochemical analysis of 5-HT and SS cells
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Serra-Campos, A. O.

; Abreu-Junior, A. N. G.

; Nascimento, A. A.

; Abidu-Figueiredo, M.

; Lima, M. S. C. S.

; Machado-Santos, C.

Brazilian Journal of Biology

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2023, Volume 83 elocation e242086

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Os objetivos do trabalho foram descrever a estrutura histológica e histoquímica do tubo gastroesofágico da Iguana iguana e verificar a ocorrência e distribuição de células serotonina (5-HT) e somatostatina (SS) imunorreativas. Fragmentos do trato gastrointestinal (TGI) de cinco iguanas foram submetidos à técnica histológica e imunohistoquímica padrão. As células imunorreativas para 5-HT e SS foram quantificadas usando o STEPanizer. O esôfago apresenta epitélio pseudoestratificado colunar ciliado Alcian blue (AB) positivo, com células caliciformes altamente reativas ao ácido periódico de Schiff (PAS). No esôfago cervical, a densidade numérica de células 5-HT por unidade de área (QA [células 5-HT] / µm2) foi de 4.6x10-2 ± 2.0 e o esôfago celomático apresentou QA = 4.0x10-2 ± 1.0. O epitélio do estômago é colunar simples, PAS e AB positivo. As regiões cranial e média do estômago apresentaram (QA [células 5-HT] / µm2) = 6.18x10-2 ± 3.2 e a região caudal, QA = 0.6x10-2 ± 0.2. As células SS foram observadas apenas no estômago caudal, com densidade numérica (QA [células SS] / µm2) = 1.4x10-2 ± 0.9. Em I. iguana, foi observada variações em termos da distribuição das secreções de muco e padrão de ocorrência das células enteroendócrinas secretoras de serotonina e somatostatina no TGI, o que possivelmente reflete uma resposta adaptativa interespecifica.

Abstract The work aims were to describe the histological and histochemical structure of the gastroesophageal tube of Iguana iguana and verify the occurrence and distribution of immunoreactive serotonin (5-HT) and somatostatin (SS) cells. Fragments of the gastrointestinal tract (GIT) of five iguanas were which underwent standard histological and immunohistochemistry technique. Immunoreactive cells for 5-HT and SS were quantified using the STEPanizer. The oesophagus has ciliated columnar pseudostratified epithelium with staining Alcian blue (AB) + and goblet cells highly reactive to periodic acid Schiff (PAS). In the cervical oesophagus, the numerical density of 5-HT cells per unit area (QA [5-HT cells]/µm2) was 4.6x10-2 ± 2.0 and celomatic oesophagus presented QA = 4.0x10-2 ± 1.0. The epithelium of the stomach is simple columnar, PAS and AB +. The cranial and middle regions of the stomach presented (QA [5-HT cells]/µm2) = 6.18x10-2 ± 3.2 and the caudal region, QA = 0.6x10-2 ± 0.2. The SS cells were only observed in the caudal stomach, with numerical density (QA [SS cells]/µm2) = 1.4x10-2 ± 0.9 In I. iguana, variation was observed in terms of the distribution of mucus secretions and the pattern of occurrence of serotonin and somatostatin-secreting enteroendocrine cells in the TGI, which possibly will result in an interspecific adaptive response.

https://doi.org/10.1590/1519-6984.242086 370 downloads

13.

Internet of Things as a Tool for Sustainable Analytical Chemistry: A Review
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Cadeado, Alegre N. S.

; Machado, Caio C. S.

; Oliveira, Geandre C.

; Silva, Daniela A. S e

; Muñoz, Rodrigo A. A.

; Silva, Sidnei G.

Journal of the Brazilian Chemical Society

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Jul 2022, Volume 33 Nº 7 Páginas 681 - 692

Resumo: En | Texto: En | PDF: En

Sustainable analytical methods are highly demanded in the modern society. Within the green principles, novel procedures that attend the sustainable development goals have been proposed and the internet of things (IoT) can play key role to achieve this goal. The association of IoT with analytical chemistry enables the real-time obtaining of analytical data to control industrial processes as well as for monitoring different environmental scenarios and human health by accessing remotely analytical information. In this context, this review presents the main IoT technologies used for analytical chemistry as well as a tutorial for beginners in the field.

https://doi.org/10.21577/0103-5053.20220048

14.

MIMO-PLC Communications in an Experimental Medium Voltage Network: Measurement and Analysis
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Vitor, Ulysses R. C.

; Cabral, Matheus

; Fonseca, Daniel

; Novo, Lauro R. G. S. L.

; Melo, Marcos T. de

; Segatto, Marcelo E. V.

; Machado, Gabriel G.

Journal of Microwaves, Optoelectronics and Electromagnetic Applications

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Mar 2022, Volume 21 Nº 1 Páginas 102 - 113

Resumo: En | Texto: En | PDF: En

Abstract This paper presents measurements at a mock-up medium voltage network to verify the feasibility of Broadband Power Line Communication (BPLC). We use the data extracted within the frequency range 2 − 30 MHz to characterize a MIMO PLC network using different phase combinations, defining the best and worst use case scenarios. The empirical data shows that not only phases directly connected for input-output can be used, but also that we can exploit the coupling between phases to transmit and receive data, therefore creating a dynamic PLC network which will depend on the characteristics of the power grid. By making use of transmission line theory, we also demonstrate how we can mitigate the reflections by measuring the input impedance of the network to allow better use of the bandwidth available for BPLC operation.

https://doi.org/10.1590/2179-10742022v21i11380

15.

DESIGN SPECIFICATIONS OF AN AUTONOMOUS ELECTRIC VEHICLE FOR USE IN FAMILY FARM UNITS
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Rosales, Jean C.

; Machado, Roberto L. T.

; Machado, Antônio L. T.

; Andrade, Henrique G.

; Kroessin, Laís

Engenharia Agrícola

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2022, Volume 42 Nº 3 elocation e20210220

Resumo: En | Texto: En | PDF: En

ABSTRACT Family farming in Brazil is responsible for the country’s food security, ensuring most of the food consumed by Brazilians. However, the agricultural machinery and equipment industry has not been dedicated to providing innovations to this sector, focusing mainly on supplying large agribusiness properties with the most advanced technology equipment. Thus, the introduction of new technological solutions regarding agricultural machines for family farming is necessary. Considering the need to supply this branch of the Brazilian agricultural sector with new technologies, this study aimed to establish the design specifications of an autonomous electric vehicle that serves as a multifunction platform, which was primarily designed to remove weeds, mainly meeting the needs of family farmers. The product development methodology in its informational phase was applied to obtain and study these needs. As a result, 19 design specifications were established with their associated target values, allowing defining the physical and economic parameters that will be the basis for the development of the autonomous electric vehicle in its conceptual phase.

https://doi.org/10.1590/1809-4430-eng.agric.v42n3e20210220/2022

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