Sludges generated in the biological processing of sewage are complex mixtures, the constituents of which pose risks to public health and the environment. Anaerobic digestion is considered the most sustainable option for treating sludge because it offers the possibility of generating biogas. The aim of this study was to compare the quantities, properties, biodegradabilities and biochemical methane potentials (BMP) of primary sludge (PS) generated by a primary decanter with acidogenic sludges produced by upflow anaerobic (UA) reactors operating at solids retention times (SRTs) of 2, 4, 6 and 8 days (Samples S2, S4, S6 and S8, respectively). Sludges from both pre-treatments were submitted to alkaline solubilization in order to determine the efficiency of the process in disrupting extracellular complexes. Based on the levels of total solids (TS) present, the primary decanter was found to generate higher quantities of excess sludge (yield of 3.1 gTS∙d−1) than UA reactors operating at low SRTs (yields in the range 1.69 to 0.64 gTS∙d−1). The concentrations of dissolved materials in PS and Samples S2 and S8 were considerably higher after alkaline solubilization, with respective increases of 8, 14 and 28-fold in dissolved organic carbon, 12, 20 and 40-fold in chemical oxygen demand, 25, 31 and 59-fold in proteins, and 17, 21 and 63-fold in carbohydrates. In addition, the BMP value for S8 was some 13% higher than that recorded for PS while the kinetic constant for gas production by S8 was 1.8-fold greater than that of PS. It is concluded that a pre-treatment combining anaerobic digestion at low SRT and alkaline solubilisation would lead to improved performance in subsequent stages of anaerobic digestion and, consequently, increased efficiency in biogas production.

RESUMO As cochonilhas-farinhentas (Hemiptera: Pseudococcidae) são consideradas pragas de importância econômica em cultivos de videira. São altamente polífagas, alimentando-se de plantas hospedeiras de 250 famílias. Objetivou-se identificar plantas hospedeiras de cochonilhas-farinhentas em cultivos de videira, na região do Vale do São Francisco. Foram amostradas plantas daninhas, ornamentais, quebra-ventos, frutíferas e nativas do bioma Caatinga. Foram identificadas 37 espécies de plantas hospedeiras de cochonilhas-farinhentas, distribuídas em 18 famílias. A família Malvaceae foi a mais frequente, com oito espécies hospedeiras identificadas, seguida de Fabaceae com quatro e Euphorbiaceae com três. A maior parte destas plantas hospedeiras está sendo aqui registrada pela primeira vez em associação com cochonilhas-farinhentas. Das espécies de plantas identificadas, 24 são hospedeiras de Maconelicoccus hirsutus, 16 de Phenacoccus solenopsis, uma de Ferrisia virgata, uma de Dysmicoccus brevipes e uma de Planococcus citri. Os resultados obtidos são importantes para melhor conhecimento da diversidade de hospedeiros de cochonilhas-farinhentas, visando à implantação de programas de manejo integrado.

ABSTRACT Mealybugs (Hemiptera: Pseudococcidae) are considered pest insects of economic importance in grapevine crops. They are highly polyphagous, feeding on host plants of about 250 families. This study aimed to identify host plants for mealybugs in grapevine crops, in the São Francisco Valley region, Brazil. The samples included weeds, ornamentals, windbreaks, fruit trees and native plants of the Caatinga biome. A total of 37 species of host plants for mealybugs were identified, distributed in 18 families. The Malvaceae family was the most frequent one, with eigth host species identified, followed by Fabaceae with four and Euphorbiaceae with three. Most of these host plants are being recorded here for the first time in association with mealybugs species. From the identified plant species, 24 are hosts for Maconelicoccus hirsutus, 16 for Phenacoccus solenopsis, one for Ferrisia virgata, one for Dysmicoccus brevipes and one for Planococcus citri. The obtained results are important to better understand the host plant diversity for mealybugs, in order to implement integrated pest management programs.

This study evaluated the biomass flow of massai grass in regrowth subjected to different nitrogen levels (control - no fertilization; 400; 800; and 1200 N kg ha-1year-1) and under rotational stocking with sheep, in a completely randomized design with repeated measures over time. The leaf elongation rate increased with increasing nitrogen levels (N) and the fourth grazing cycle presented a lower value compared with the others. The stem elongation rate responded linearly with increasing levels of N, but it was not influenced by grazing cycles. The senescence rate of leaves remaining before grazing and after grazing were not influenced by the nitrogen fertilization or amended with successive grazing cycles. The phyllochron was influenced only by the nitrogen fertilization, with a decreasing linear response with nitrogen levels. Quadratic response was observed for the average length of leaves with increasing N levels and the grazing cycle 4 presented the lowest value for this variable. For each kilogram N ha-1 year-1, increases of 0.161 and 0.1604 kg ha-1 day-1 were verified in the herbage growth and accumulation rates, respectively. Nitrogen fertilization favors the biomass flow of massai grass, promoting an expressive increase in the production and accumulation of forage.

OBJECTIVE: To assess, in myocardium specimens obtained from necropsies, the correlation between the concentration of hydroxyproline, measured with the photocolorimetric method, and the intensity of fibrosis, determined with the morphometric method. METHODS: Left ventricle myocardium samples were obtained from 45 patients who had undergone necropsy, some of them with a variety of cardiopathies and others without any heart disease. The concentrations of hydroxyproline were determined with the photocolorimetric method. In the histologic sections from each heart, the myocardial fibrosis was quantified by using a light microscope with an integrating ocular lens. RESULTS: A median of, respectively, 4.5 and 4.3 mug of hydroxyproline/mg of dry weight was found in fixed and nonfixed left ventricle myocardium fragments. A positive correlation occurred between the hydroxyproline concentrations and the intensity of fibrosis, both in the fixed (Sr=+0.25; p=0.099) and in the nonfixed (Sr=+0.32; p=0.03) specimens. CONCLUSION: The biochemical methodology was proven to be adequate, and manual morphometry was shown to have limitations that may interfere with the statistical significance of correlations for the estimate of fibrosis intensity in the human myocardium.