Abstract Objective: To audit our experience with Choanal Atresia (CA). Study design: Observational retrospective study. Material/Methods: Clinical data of all CA patients followed from 2003 to 2020 at Hospital Central do Funchal were analysed. Results: Eleven patients were included and 63,64% were females. They were divided into two groups: Unilateral (63,64%) and bilateral atresia (36,36%). In both groups, mixed type atresia was the most common. Regarding unilateral group, endoscopic repair was performed in 85,71% of cases (71,4% with stents) at a median age of 15 years old. Median number of surgeries was 1,29. Bilateral group had more congenital malformations (50%) and had more reinterventions. All patients underwent an endoscopic approach at a median age of 8 days old. Conclusions: Associated malformations, younger age at first surgery and higher number of surgeries were more associated to bilateral CA.

Resumo Objetivo : Descrever a experiência do Serviço de Otorrinolarinogologia do Hospital Dr. Nélio Mendonça com Atrésia das Coanas (AC). Desenho do estudo: Observacional retrospectivo. Material/Métodos: Analisaram-se os dados clínicos de todos os doentes com AC de 2003-2020 do Hospital Central do Funchal. Resultados: Dos onze doentes incluídos, 63,64% eram do sexo feminino e 36,36% eram do sexo masculino. As onze crianças estudadas foram divididas em dois grupos: AC unilateral (63,64%) e bilateral (36,36%). Nos dois grupos, o tipo misto foi o mais comum. Na AC unilateral, a correção endoscópica foi realizada em 85,71% dos casos (71,4% com stents) com idade média de 15 anos. Foi realizada uma média de 1,29 cirurgias. O grupo bilateral apresentou mais malformações congénitas (50%) e apresentou mais reintervenções. Todos foram submetidos a cirurgia endoscópica (idade média: 8 dias). Conclusões: Malformações associadas, idade mais jovem na primeira cirurgia e maior número de cirurgias estiveram mais associadas à AC bilateral.

ABSTRACT Plantation forests can be efficient C sinks in biomass and soil organic carbon (SOC), but the latter depends on many factors, including climate. Tropical humid, mountain areas have cooler temperatures, slowing microbial decomposition, and thus can store considerable SOC. However, the effects of forest plantations on SOC of these montane areas are still poorly studied. Here, we aimed to assess changes in SOC, and related soil properties, after conversion of native rainforest to plantations of five tree species, with rotation cycles varying from 7 to 30 years, on the Mantiqueira Range, Minas Gerais, Brazil. We measured SOC contents and stocks (0.00-0.40 m layer) under a native montane rainforest (control) and plantations of Eucalyptus, Pinus, Cunninghamia, Cupressus and Araucaria, all planted in 3 × 3 m spacing, at an altitude of ca. 1,300 m, marked by humid and cool climate, where SOC contents are naturally high. Soil organic carbon varied from 55 g kg-1 under Eucalyptus to 105 g kg-1 under Araucaria (0.00-0.05 m layer), decreasing in depth (0.20-0.40 m) to the still high values of 20-40 g kg-1. Soil organic carbon stocks for the top 0.20 m were also high, reaching ca. 140 Mg ha-1 under Araucaria, significantly higher value than the native forest (ca. 90 Mg ha-1, p<0.05), which did not differ from the other species. Soil organic carbon stocks were not affected in the 0.20-0.40 m soil layer, whereas soil structure patterns changed under some species, without however resulting in bulk density changes, and pH decreased under Araucaria. Such data showed large SOC stocks under montane native forests can not only be preserved upon conversion to forest plantations, but considerable SOC sequestration can be achieved in 30-years rotation cycles plantations of indigenous Araucaria angustifolia, marked by more open canopies and greater understory biomass. , (SOC) factors climate temperatures decomposition However studied Here properties species years Range Gerais Brazil 0.000.40 000040 0.00 0.40 0 00 40 (0.00-0.4 layer control (control Pinus Cunninghamia spacing ca 1300 1 300 1,30 5 kg1 kg kg- 10 0.000.05 000005 0.05 05 (0.00-0.0 0.200.40 020040 20 (0.20-0.4 2040 20-4 kg1. 1. 020 0.2 14 ha1 ha ha- (ca 9 ha1, 1, p<0.05, p005 p p<0.05 p<0.05) 0.20-0.4 30years angustifolia (SOC 000 0.000.4 00004 0.0 040 0.4 4 (0.00-0. 130 1,3 0.000.0 00000 005 200 0.200.4 02004 2 (0.20-0. 204 20- 02 0. p00 p<0.0 0.20-0. 0.000. 0000 04 (0.00-0 13 0.200. 0200 (0.20-0 p0 p<0. 0.20-0 0.000 (0.00- 0.200 (0.20- p<0 0.20- (0.00 (0.20 p< (0.0 (0.2 (0. (0 (

In this work, cellulose-based composite biofilms doped with silver nanoparticles (AgNPs) have been produced by eco-friendly process. The AgNPs were synthesized from Ilex paraguariensis with an average size of 45 nm, a characteristic plasmon band at 450 nm and zeta potential of -23 mV. The biofilm has been synthesized by casting method, using different volumes of AgNPs suspension with glycerol plasticizing agent (T6-T10) or not (T1-T5). The AgNPs were successfully incorporated into the biofilms, which was confirmed by scanning electron microscopy. The mechanical and optical structural and the antimicrobial properties of the biofilms have been determined. In the presence of AgNPs, the mechanical properties, thermal stability and water vapor barrier of the biofilms have not been altered. However, the addition of glycerol decreased the tensile strength and the modulus of elasticity, in addition to increasing the elongation of the biofilms and the water vapor barrier. The biofilm T10 showed a transmittance of ca. 10%, with high potential to be used as a UV light blocker. Finally, the biofilms doped with AgNPs showed activity against the bacterium Pseudomonas fluorescens. Given the above, this work presented a new biofilm with activity against the bacterium Pseudomonus fluorescens obtained by eco-friendly process. cellulosebased cellulose based (AgNPs ecofriendly eco friendly process 4 23 -2 mV method T6T10 TT T6 T (T6-T10 T1T5. T1T5 T1 T5 . (T1-T5) microscopy determined altered However elasticity ca 10 10% blocker Finally above 2 - T6T1 (T6-T1 T1T (T1-T5 1 T6T (T6-T (T1-T

Florpyrauxifen-benzyl is the new active ingredient authorized by Brazilian legislation, which acts as herbicide and may be used to control weeds in rice cultivation, but studies on toxicity to humans and the environment are still under review. Due to recent insertion in the world pesticide market, there are still few studies related to the extraction and detection methodologies of this compound. Therefore, this study aimed to optimize and validate solid-liquid extraction with low temperature purification (SLE-LTP) for determining florpyrauxifen-benzyl in soil using high-performance liquid chromatography coupled to a diode array detection (HPLC-DAD). The results showed that the best conditions were achieved using Poroshell column, a temperature of 30 °C, mobile phase composition of acetonitrile:water (85:15 v/v) acidified with formic acid 0.1% (v/v), a flow rate of 0.3 mL min-1, and 243 nm as the wavelength. The best extracting phase was acetonitrile acidified with formic acid 0.1% (v/v) which achieved a recovery rate ca. 100% and relative standard deviation (RSD) less than 3.9%. The methodology was precise, accurate, linear and selective, with a limit of quantification of 20 µg kg-1. The stability study of this compound in soil showed that approximately 17.5 days is the half-life of florpyrauxifen-benzyl in soil. Florpyrauxifenbenzyl Florpyrauxifen benzyl legislation cultivation review market Therefore solidliquid solid SLELTP SLE LTP (SLE-LTP florpyrauxifenbenzyl florpyrauxifen highperformance high performance HPLCDAD. HPLCDAD HPLC DAD . (HPLC-DAD) column 3 C °C acetonitrilewater water 8515 85 15 (85:1 v/v vv v 01 0 1 0.1 v/v, , 03 0. min1, min1 min 1, min-1 24 wavelength (v/v ca 100 RSD (RSD 39 9 3.9% precise accurate selective 2 kg1. kg1 kg 1. kg-1 175 17 5 17. halflife half life (HPLC-DAD 851 8 (85: min- 10 3.9 kg- (85 3. (8 (

In this work, an iron/biochar composite was evaluated as a catalyst for 3,6-dichloro-2-methoxybenzoic acid (dicamba) herbicide degradation by heterogeneous Fenton-like process. The biochar was produced from pyrolysis of coffee husks. After FeII adsorption by biochar, the material was submitted to chemical reduction, via borohydride, producing zero-valent iron nanoparticles (BIO-Fe0). The material was characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy and energy dispersive X-ray spectroscopy, Raman scattering spectroscopy, nitrogen adsorption/desorption analysis, among other techniques. The BIO-Fe0 is a heterogeneous material, and the main constituent elements are carbon (C), oxygen (O), silicon (Si), sulfur (S) and iron (Fe). The dicamba degradation was monitored by high-performance liquid chromatography and biological assays using beans (Phaseolus vulgaris) as indicator species. The commercial dicamba (500 mL; 250 mg L 1) was submitted to Fenton-like process (initial pH 3.20; BIO-Fe0: 3 or 5 g; 1.00 mL H2O2 at 3.00 mmol L 1; 1.00 mL Al2(SO4)3 at 1.00 mol L 1; 25 ºC and 5 h of reaction), presenting ca. 100% of degradation. These treated solutions were used in a biological assay. The intoxication levels were less 30%. These results show that BIO-Fe0 has the potential to be used in the dicamba degradation by Fenton-like processes. work ironbiochar 3,6dichloro2methoxybenzoic 36dichloro2methoxybenzoic dichloromethoxybenzoic 3,6 dichloro 2 methoxybenzoic 6 (dicamba Fentonlike Fenton like husks reduction borohydride zerovalent zero valent BIOFe0. BIOFe0 BIOFe BIO Fe0 . Fe (BIO-Fe0) FTIR, FTIR , (FTIR) Xray X ray adsorptiondesorption desorption analysis techniques BIO-Fe C, C (C) O, O (O) Si, Si (Si) S (S Fe. (Fe) highperformance high performance Phaseolus vulgaris species 500 (50 1 initial 3.20 320 20 g 100 00 1.0 HO H H2O 300 3.0 Al2SO43 AlSO Al2 SO4 Al SO Al2(SO4) reaction, reaction reaction) ca assay 30 30% processes 6dichloro2methoxybenzoic 36 3, (BIO-Fe0 (FTIR (C (O (Si (Fe 50 (5 3.2 32 10 0 1. 3. Al2SO4 Al2(SO4 (BIO-Fe ( Al2SO Al2(SO

Abstract Objectives: To determine if anterior commissure (AC) involvement in glottic cancer (GC) is a prognosis factor to recurrence and 5-year survival rate. Study design: Observational retrospective. Methods: Assessment of medical files of patients who were diagnosed with GC with T1a and T1b staging during a 12-year period (2010-2021). Results: Twenty-three patients (67,6%) were classified with T1a stage (10 cases with AC involvement) and 11 patients (32,4%) with T1b staging (7 cases with AC involvement). AC involvement was associated with higher risk of recurrence for T1b cases (p<0,05), but not for T1a cases. Five-year mortality rate had no significant statistically difference. Conclusions: AC involvement was a negative prognostic factor in local disease control during follow-up, thus raising the possibility of AC inclusion in T staging in glottic tumors.

Resumo Objetivos: Determinar se o envolvimento da comissura anterior (CA) é um fator de prognóstico no carcinoma glótico (CG) para recidiva e para sobrevida a 5 anos. Desenho de estudo: Observacional retrospetivo. Material e métodos: Revisão dos processos clínicos de doentes com diagnóstico de CG com estadiamento T1a e T1b num período de 12 anos (2010-2021). Resultados: Vinte e três doentes (67,6%) foram classificados como estadio T1a (10 casos com envolvimento da CA) e 11 (32,4%) doentes com estadio T1b (7 casos com envolvimento da CA). O atingimento da comissura anterior associou-se a risco de recidiva para os casos T1b (p<0,05), mas não para os casos T1a. A mortalidade a 5 anos não teve diferença estatisticamente significativa. Conclusões: Neste estudo, o envolvimento da CA foi fator de mau prognóstico na taxa de controlo do tumor durante o seguimento dos doentes. Os autores levantam assim a hipótese de este ser um fator a ser considerado no estadiamento T em tumores glóticos.

Resumo O sucesso da produção e da qualidade das hastes florais de gérbera depende, principalmente, dos fatores ambientais e do correto manejo da adubação. O objetivo do trabalho foi avaliar a variação da produção e da qualidade de hastes florais de gérbera de corte cultivada em substrato à base de casca de pinus durante 28 meses (sendo 25 meses de colheita), com diferentes doses de nitrogênio (N) e cálcio (Ca). O experimento foi conduzido em ambiente protegido, e consistiu da combinação de três doses de N L-1 de substrato (0,07 g, 0,15 g e 0,2 g) e de três doses de Ca L-1 de substrato (0,02 g, 0,03 g e 0,04 g), aplicadas a cada 15 dias, além da testemunha, sem adubação com N e Ca. A maior produção e a melhor qualidade das hastes florais por planta ocorreram nos meses com temperatura do ar média entre 20 ºC e 25 ºC. A produção de hastes florais planta-1 mês-1 e o diâmetro do capítulo tiveram os máximos valores obtidos com 0,14 g de N L-1 de substrato. Já para as doses de Ca, observou-se um ajuste linear positivo significativo, ou seja, os maiores valores foram obtidos com a dose de 0,04 g de Ca L-1 de substrato. Ocorre variação mensal da produção e da qualidade das hastes florais de gérbera de corte cultivada em substrato comercial, à base de casca de pinus, e é independente do uso de diferentes doses de nitrogênio e de cálcio.

Abstract Success in production and quality of gerbera flower stalks over time depends on environmental factors and on appropriate fertilization procedures. The objective of the present work was to evaluate the production and quality of flower stalks of gerberas as cut flower cultivated for 28 months (with 25 months of harvest) in a pine bark-based substrate supplemented with different doses of nitrogen and calcium. The experiment was conducted in a greenhouse. Three doses of nitrogen (N) (0.07 g, 0.15 g and 0.20 g L-1 substrate) and three doses of calcium (Ca) (0.02 g, 0.03 g and 0.04 g L-1 substrate) were applied every 15 days along the cultivation period. The control was considered an additional treatment to which neither N nor Ca was supplemented to the plants in the pots. Highest flower yields and best flower quality were obtained in months in which the temperatures were in the range of 20 ºC to 25 ºC. The highest number of floral stalks and the largest diameter of the capitulum had a significant quadratic adjustment according to N doses. The maximum values were obtained with 0.14 g of N L-1 substrate. Whereas for Ca fertilization, a significant positive linear adjustment was determined, i.e., the highest values were obtained with the highest dose (0.04 g Ca L-1 substrate). There was a monthly variation in the yield and quality of cut gerberas grown in commercial substrate based on pine bark regardless of the different doses of nitrogen and calcium used in the experiment.

Abstract The majority of ornithogenic soils studied in Antarctica focus on the influence of penguins, wherever little reports evaluated the influence of flying birds on soil genesis. This study aimed to characterize the morphologic, chemic, physic, mineralogic, and micromorphologic ornithogenic soil pockets influenced by flying birds in Snow Island, Maritime Antarctica. Fifteen soil pockets were selected, described, sampled and analyzed, these sites constitute the main areas with intense long-term terrestrial biological activity in Snow Island. In order to investigate the impact of phosphatization, we compared the soil pockets with the surrounding soils and soils affected by penguins. Zone of phosphatization have a high concentration of P, K, and Ca. The XRD patterns for the clay fraction of ornithogenic soils show that phosphate minerals are the main crystalline phases (leucophosphite, minyulite, fluorapatite, and apatite). We show that even under typical periglacial conditions, sites influenced by flying birds present active chemical weathering processes. The phosphatization release exchangeable bases and accelerate mineralogical and micromorphological transformations in soils. Under the current global warming trend and expected sea-level rise, the ornithogenic environments are susceptible to accelerated erosion rates and a great part of these hotspots may be lost for the open sea.

Abstract Scinax comprises 129 species of treefrogs divided between the S. ruber and the S. catharinae clades. The S. rostratus group belongs to the S. ruber clade, and comprises ten species characterized by having a dark interorbital triangular mark, tubercle on the heel and by vocalizing in a head-down position. Within this group, S. kennedyi and S. rostratus are similar in that they are the only species that do not possess a row of tubercles on the lower jaw. In spite of their morphological similarity, they can be distinguished from each other by male size and advertisement call traits. Scinax kennedyi was described from eastern Colombia, and occurs from central Colombia to western Venezuela, whereas S. rostratus was described from northern Venezuela, and is thought to occur from Panama to eastern Venezuela, and in the Brazilian state of Pará. A recent study reported a population identified as S. rostratus from the state of Roraima, in northern Brazil. However, this population presented substantial differences in male size and advertisement call in comparison to the described for this species, and apparently it best fits the diagnosis of S. kennedyi instead. In view of this, the present study aimed to clarify the identity of this Brazilian population through a reassessment of its specimens and call recordings, together with the examination of data of S. kennedyi from its type locality, and of S. rostratus from northern Venezuela. Acoustic and morphological analyses revealed that specimens from northern Brazil were indeed misidentified as S. rostratus, and hence they were herein reassigned to S. kennedyi. The study therefore demonstrates the occurrence of S. kennedyi in Brazil, extending its distribution ca. 1,120 km east by south from its type locality, and ca. 844 km east-southeast from its easternmost previous record. With this reassessment, a clearer acoustic diagnosis between S. kennedyi and S. rostratus was provided, as well as further data on fine-scale temporal traits of their calls.

RESUMO Objetivo: Analisar a composição dos macronutrientes presentes no leite de mães de recém-nascidos pré-termo (RNPT) - gorduras, carboidratos e calorias - por idade gestacional (IG), idade cronológica (IC) e variáveis maternos. Métodos: Estudo longitudinal para analisar 215 amostras de leite de 51 mães de RNPT admitidos em três unidades neonatais do Rio de Janeiro de maio/2013 a janeiro/2014. A coleta de leite foi realizada por bomba coletora, em dia fixo a cada semana até a alta. Utilizou-se a técnica espectrofotométrica com análise de infravermelho (MilkoScan Minor 104) para a análise quantitativa. Uma amostra de 7 mL de leite humano foi retirada do volume total de leite extraído pela mãe. Os dados foram agrupados por IG (25-27, 28-31, 32-36 e 37-40 semanas) e por IC (0-4, 5-8, 9-12 e 13-16 semanas). Resultados: Proteínas, carboidratos, gorduras e calorias não apresentaram nenhum padrão de mudança, não havendo diferença entre os grupos de IG. Quando os macronutrientes foram analisados por grupos de IC, a proteína diminuiu, com diferença significante entre os dois primeiros grupos de IC. Carboidratos, gorduras e calorias apresentaram valores crescentes em todos os grupos, sem diferença estatística. O ganho de peso durante a gestação, a presença de hipertensão arterial e a idade materna foram associados a alterações de gordura e calorias no primeiro momento da análise do leite. Conclusões: Observou-se redução estatisticamente significante nos níveis de proteína durante as primeiras oito semanas após o nascimento. A IC pode ser um fator importante na composição do leite humano.

ABSTRACT Objective: To analyze the composition of macronutrients present in the milk of mothers of preterm newborn infants (PTNB) - protein, fat, carbohydrate, and calories - by gestational age (GA), chronological age (CA) and maternal variables. Methods: Longitudinal study that analyzed 215 milk samples from the 51 mothers of PTNB admitted in three Neonatal Intensive Care Units of Rio de Janeiro from May/2013-January/2014. Milk collection was performed by pickup pump, on a fixed day of each week until discharge. The spectrophotometric technique with Infrared Analysis (MilkoScan Minor 104) was used for the quantitative analysis. A sample of 7 mL of human milk was taken from the total volume of milk extracted by the mother. The data was grouped by GA (25-27, 28-31, 32-36, 37-40 weeks) and by CA (zero to 4, 5-8, 9-12, 13-16 weeks). Results: Protein, carbohydrate, fat and calories did not show any pattern of change, with no difference among groups of GA. When the macronutrients were analyzed by groups of CA, protein decreased, with significant difference between the first two groups of CA. Carbohydrates, fat and calories presented increasing values in all groups, without significant differences. Weight gain during pregnancy, maternal hypertension and maternal age were associated with changes in fat and calories in the first moment of the analysis of milk. Conclusions: There was a significant decrease in the levels of protein during the first eight weeks after birth. CA may be an important factor in the composition of human milk.

Resumo O angiofibroma celular é um tumor mesenquimatoso benigno e raro. Nasmulheres, surge principalmente na região vulvo-vaginal, com localização vulvar em 70% dos casos. A sua apresentação clínica é inespecífica e semelhante a vários outros tumores vulvares de diferentes origens celulares. Assim, são as suas características histológicas e imunohistoquímicas que permitem a diferenciação entre eles. Os angiofibromas celulares têm bom prognóstico,embora apresentemalgumrisco de recidiva. Os autores apresentamo caso de umamulher de 49 anos de idade comuma lesão vulvar direita volumosa, cujo diagnóstico pré-operatório era de quisto da glândula de Bartholin, mas cujo exame histológico e imunohistoquímico revelaram tratar-se de um angiofibroma celular.

Abstract Cellular angiofibroma (CA)is a rare benign mesenchymal tumor. In women, it occurs mainly in the vulvovaginal region, with vulvar location in 70% of the cases. Its clinical presentation is nonspecific and similar to several other vulvar tumors of different cellular origins. Thus, its histological and immunohistochemical features allow distinction fromother tumors. Cellular angiofibromas have good prognosis, despite some risk of relapse. The authors present the case of a 49-year-old woman with a bulky right vulvar lesion, for which the preoperative diagnosis was a Bartholin cyst, but the histological and immunohistochemical evaluation yielded a CA.

Photophysical processes in multicomponent solutions composed by natural pigments, extracted from Caesalpinia echinata (Brazil-wood) leaves, are investigated. Beacuse of the main fluorescent molecules in these solutions are the chlorophyll a, chlorophyll b and pheophytin a, all features are discussed regarding these three pigments. It is observed that the fluorescence bands of these macrocyclic molecules, located around ca. 673 nm, are progressively quenched due to the increase of the solutions’ concentration. Due to a significant overlapping between their fluorescence and absorption Q-bands, it is concluded that the inner-filter effect causes such quenching. Through analysis of steady-state and time-resolved fluorescence, it is verified that the inner filter acts more effectively on the emission assigned to chlorophyll a. Invoking reported extinction coefficients for these pigments in their isolated forms, we can give a qualitative interpretation of such selectivity considering the effectiveness of the overlapping between the absorption and fluorescence bands.

Resumo: O conhecimento das assembleias de répteis para muitos ecossistemas no Brasil pode ser considerado ainda relativamente restrito. Neste estudo, nós realizamos amostragens no Monumento Natural Serra das Torres, localizado nos municípios de Atílio Vivacqua, Muqui e Mimoso do Sul, no estado do Espírito Santo, utilizando o método de avaliação rápida (RAP) durante 30 dias na estação chuvosa de 2018. Amostramos cerca de 1320 horas de busca ativa durante os períodos diurno e noturno, com uma equipe de 6 a 10 pessoas, suplementada por 720 horas de amostragem com armadilhas de queda com cercas guia (30 dias-balde). Registramos 34 espécies de répteis squamatas durante as amostragens (2 anfisbenídeo, 11 lagartos e 21 serpentes) e um encontro ocasional posterior que acrescentou uma espécie de quelônio à lista, Hydromedusa maximiliani, totalizando 35 espécies de répteis. Dipsadidae foi a família com a maior riqueza de serpentes, e Gymnophtalmidae foi a família com maior riqueza de lagartos. A riqueza de espécies que registramos no Monumento Natural Serra das Torres (Ntotal = 35) representa ca. 27% de todas as espécies de répteis encontradas no estado do Espírito Santo (N = 130). A espécie de lagarto mais abundante foi Leposoma scincoides seguido por Ecpleopus gaudichaudii, enquanto a espécie mais abundante de serpente foi a Bothrops jararaca, sendo marcadamente maior do aquela registradas em estudos similares. Vinte e sete por cento das espécies de registradas em nosso estudo são endêmicas da Mata Atlântica e trinta por cento das espécies (N = 10) tinham menos de cinco indivíduos registrados anteriormente no estado do Espírito Santo. Nosso estudo reforça a necessidade de conservação do Monumento Natural Serra das Torres devido à sua importância como reservatório de uma considerável parcela da biodiversidade de répteis do estado do Espírito Santo, bem como do bioma Mata Atlântica.

Abstract: Data on the composition of local reptile assemblages in several Brazilian ecosystems can still be considered relatively restricted in scope in most cases. In this study, we conducted surveys in the Serra das Torres Natural Monument, located in the municipalities of Atílio Vivacqua, Muqui, and Mimoso do Sul, using the Rapid Assessments method (RAP) during 30 days in the rainy season of 2018. We sampled actively for approximately 1320 hours with a 6-10 person crew, supplemented by 720 hours of passive sampling (30 bucket-days) using pitfall traps with drift fences. We recorded 34 reptile species during our sampling method (2 amphisbaenid, 11 lizards, and 21 snakes) and an occasional encounter, after the end of sampling, that added a chelonian species to the list, Hydromedusa maximiliani, totaling 35 reptile species. The Dipsadidae was the family with the greatest snake species richness and, the Gymnophtalmidae had the greatest lizard species richness. The species richness recorded in the Serra das Torres Natural Monument (Ntotal = 35) represents ca. 27% of all reptile species found in the state of Espírito Santo (N = 130). The most abundant lizard species was Leposoma scincoides followed by Ecpleopus gaudichaudii and, the most abundant snake species was Bothrops jararaca being markedly higher than that recorded in similar studies. Twenty-seven percent of the reptile species recorded in our study are endemic to the Atlantic Forest and 30% (N = 10) have been recorded less than five times previously in the Brazilian state of Espírito Santo. Our study reinforces the need for the conservation of the Serra das Torres Natural Monument because of its importance as a reservoir of a considerable portion of the reptile biodiversity of Espírito Santo state, and of the Atlantic Forest biome as a whole.

Abstract Rhagionidae (Brachycera, Tabanomorpha) is a widespread family of the order Diptera, containing ca. 720 species in 22 genera worldwide distributed. Chrysopilus Marquart is one of the most speciose genus of Rhagionidae. Here we describe Chrysopilus kafkai sp. nov. Cegolin & Santos, the first record of a rhagionid species from Midwest Brazilian region, and includes an identification key and a distribution map to the Brazilian species of Chrysopilus. The species description is complemented by a molecular characterization of the 28S rRNA sequence.

Abstract New records for Helicina schereri Baker, 1913, are reported. It was a species previously restricted to the states of Ceará, Rio Grande do Norte, Paraíba, Alagoas and Tocantins in Northeastern Brazil, and State of Santa Catarina, much further south. The new occurrences reported herein fill distribution gaps and also significantly expand the range of the species ca. 970 km westwards. The new records are from the following locations: Bahia State (Ituaçu and Itaquara municipalities) in Northeast Brazil; Mato Grosso do Sul State (Bonito Municipality) in the Midwest; and Minas Gerais (Lagoa Santa municipality) in the Southeast.