Abstract Objective: Studies focusing on bone and joint infections (BJIs) in young infants are rare. Some cases of BJI are accompanied by sepsis. This study aimed to identify the clinical and bacteriological features of sepsis in neonates and young infants with BJIs. Methods: Neonates and infants younger than 3 months diagnosed with BJI in the present institution from 2014 to 2021 were retrospectively reviewed. Patient characteristics, clinical data, and outcomes were documented and compared between those with and without sepsis. Results: Twenty-five patients with a mean age of 34.8 days were included. Nine BJI cases had concomitant sepsis (group A), and 16 had BJI without sepsis (group B). Within group A, staphylococcus aureus was the major pathogenic germ (5 cases, of which 4 were of the methicillin-resistant staphylococcus aureus (MRSA) type). There was no statistical difference in male-to-female ratio, age, history of hospitalization, anemia, birth asphyxia, peripheral leukocyte counts, C-reactive protein on admission, and sequelae between groups. Univariate analyses indicated a significant difference in the incidence of septic arthritis (SA) combined with osteomyelitis (OM) (88.9% vs 37.5%), congenital deformities (44.4% vs 0%), and mean duration of symptoms (2.83 days vs 9.21 days) in comparisons between groups A and B. Conclusion: Staphylococcus aureus is the main pathogenic bacteria in BJI cases complicated with sepsis in neonates and young infants. Among infants younger than 3 months diagnosed with BJI, those with concurrent SA and OM, MRSA infection, or congenital deformities are more likely to develop sepsis. Objective BJIs (BJIs rare Methods 201 202 reviewed characteristics data Results Twentyfive Twenty five 348 34 8 34. included , A) 1 B . B) 5 ( methicillinresistant methicillin resistant (MRSA type. type type) maletofemale male female ratio hospitalization anemia asphyxia counts Creactive C reactive admission (SA OM (OM 88.9% 889 88 9 (88.9 37.5%, 375 37.5% 37 37.5%) 44.4% 444 44 (44.4 0%, 0 0% 0%) 2.83 283 2 83 (2.8 921 21 9.2 Conclusion infection 20 88.9 (88. 37.5 44.4 (44. 2.8 28 (2. 92 9. 88. (88 37. 44. (44 2. (2 (8 (4

ABSTRACT Through a long-term micro-area positioning experiment (2006-2017), the precise fertilization locations of spring maize (Zea mays L.) in Jilin, China, were investigated using an embedded cement bucket pot. The effects of different fertilization placements and additional application of organic manure on maize yield, biomass, grain nutrient uptake, and soil fertility were examined. Six treatments were designed: base fertilizer + topdressing shallow application (BF1TD1); base fertilizer deep + topdressing shallow application (BF2TD1); base fertilizer + topdressing deep application (BF1TD2); base fertilizer deep + topdressing deep application (BF2TD2); base fertilizer + topdressing + organic manure shallow application (BF1TD1 + OS); and base fertilizer + topdressing shallow application + organic manure deep application (BF1TD1 + OD). After 12 years of continuous experimentation, the findings revealed that BF2TD2, BF1TD1 + OS, and BF1TD1 + OD exhibited 10.59 %, 25.17 %, and 29.34 % higher average yields than BF1TD1, respectively. Deep topdressing was more beneficial in increasing maize yield and nutrient accumulation, and additionally, increasing the use of organic fertilizer enhanced plant biomass and nutrient uptake. Over the 12 years considered, the soil organic matter in the 0-20 cm and 20-40 cm layers increased by 45.96 % and 80.61 %, respectively, and the soil pH increased from 0.38 to 0.48. In general, the deep application of organic manure was more beneficial for soil retention as it can increase soil fertility in the 0-40 cm layer. Considering the high yield and nutrient absorption and utilization of maize, technical advancement for soil fertilizer in whole-field tillage layers was crucial. longterm long term microarea micro area 20062017, 20062017 2006 2017 , (2006-2017) Zea L. L Jilin China pot uptake examined designed BFTD BF TD (BF1TD1) BF2TD1 (BF2TD1) BF1TD2 (BF1TD2) BF2TD2 (BF2TD2) (BF1TD OS OS) OD. . OD) 1 experimentation BF1TD 1059 10 59 10.5 2517 25 17 25.1 2934 29 34 29.3 respectively accumulation additionally considered 020 0 20 0-2 2040 40 20-4 4596 45 96 45.9 8061 80 61 80.6 038 38 0.3 048 48 0.48 general 040 0-4 layer wholefield whole field crucial 2006201 200 201 (2006-2017 BF2TD (BF2TD1 (BF1TD2 (BF2TD2 105 5 10. 251 2 25. 293 3 29. 02 0- 204 4 20- 459 9 45. 806 8 6 80. 03 0. 04 0.4 200620 (2006-201 (BF2TD 20062 (2006-20 (2006-2 (2006- (2006 (200 (20 (2 (

Abstract Background: Acid-sensing ion channel 1a (ASIC1a) plays a critical role in physiological and pathological processes. To further elucidate the biological functions of ASICs and their relationships with disease occurrence and development, it is advantageous to investigate and develop additional regulatory factors for ASICs. Methods: In this study, cation exchange chromatography was used to separate seven chromatographic components from Naja naja atra venom. Capillary electrophoresis was employed to detect that Ⅶ peak component containing a main protein Ⅶ-2, which could bind to ASIC1a. The analgesic effects of Ⅶ-2 protein were determined using hot plate methods, and ASIC1a expression in spinal cord tissue from rats with inflammatory pain was detected using western blot. Results: The purified Ⅶ-2 protein named Naja naja atra venom-Ⅶ-2 (NNAV-Ⅶ-2) was obtained by Sephadex G-50 gel filtration, which exhibited a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular weight of 6.7 kD. Remarkably, the NNAV-Ⅶ-2 protein demonstrated a significant analgesic effect and downregulated ASIC1a expression in the spinal cord tissue of rats with inflammatory pain. Conclusions: The analgesic mechanism of the NNAV-Ⅶ-2 protein may be associated with its binding to ASIC1a, consequently downregulating ASIC1a expression in neural tissues. Background Acidsensing Acid sensing ASICa ASIC (ASIC1a processes development Methods study venom Ⅶ2, Ⅶ2 2, 2 Ⅶ- methods blot Results venomⅦ2 venom-Ⅶ- NNAVⅦ2 NNAV (NNAV-Ⅶ-2 G50 G 50 G-5 filtration sulfatepolyacrylamide sulfate polyacrylamide 67 6 7 6. kD Remarkably NNAV-Ⅶ- Conclusions tissues venomⅦ venom-Ⅶ NNAVⅦ (NNAV-Ⅶ- G5 5 G- NNAV-Ⅶ venom- (NNAV-Ⅶ NNAV- (NNAV- (NNAV

RESUMO No setor aeronáutico, ligas de alumínio 2024 são amplamente empregadas devido à excelente resistência mecânica e baixa massa específica. No entanto, essas ligas são propensas à corrosão localizada em meios corrosivos específicos, sendo necessários métodos de tratamento para protegê-las. Inibidores a base de cromo hexavalente são eficientes, mas têm seu uso restrito devido aos riscos à saúde humana e impactos ambientais associados. Nesse sentido, filmes de polímeros condutores têm sido investigados para substituir compostos tóxicos. Neste estudo, investigou-se o desempenho de bicamadas de polipirrol na proteção da liga de alumínio 2024 contra corrosão. Os resultados dos testes de polarização potenciodinâmica, realizados em solução salina, mostraram um deslocamento dos valores de potencial de corrosão para a região mais positiva quando as superfícies de alumínio foram revestidas com o filme de polipirrol, indicando proteção anódica. Além disso, as curvas de Tafel indicaram que as densidades de corrente anódicas foram inferiores para a bicamada de polipirrol. Adicionalmente, a morfologia das superfícies analisada por Microscopia Eletrônica de Varredura demonstrou que as bicamadas foram compactas e homogêneas, permanecendo intactas mesmo após três dias de imersão em solução de cloreto, sugerindo que a bicamada de polipirrol pode proteger a superfície metálica contra corrosão. aeronáutico 202 específica entanto específicos protegêlas. protegêlas protegê las. las protegê-las eficientes associados sentido tóxicos estudo investigouse investigou se potenciodinâmica salina anódica disso Adicionalmente homogêneas cloreto 20 2

ABSTRACT In the aeronautical industry, 2024 aluminum alloys are widely used due to their excellent mechanical resistance and low specific mass. However, pitting formation is usual for these metal surfaces in certain corrosive environments, requiring treatment methods to protect them. Polypyrrole is a conductive polymer that has been studied for the protection of oxidizable metals against corrosion, as a viable alternative to toxic compounds. In this context, this study aims to investigate the performance of polypyrrole bilayers, electrodeposited in aqueous medium to protect 2024 aluminum alloy against corrosion. Potentiodynamic polarization curves, in corrosive chloride medium, indicated that the corrosion potential values were shifted towards the more positive values for the aluminum surfaces coated with polypyrrole films in relation to the surface of the metal just polished, indicating anodic protection. Furthermore, the Tafel curves indicated that the anodic current densities were lower for the polypyrrole bilayer electrodeposited in phosphoric acid and dodecylbenzenesulfonic acid, respectively. The morphology of the surfaces was investigated by Scanning Electron Microscopy and indicated that the bilayers were compact and homogeneous and that they were not damaged even after three days of immersion in chloride medium, indicating that the polymeric film can protect the metal against corrosion. industry 202 mass However environments them compounds context polished Furthermore respectively 20 2

Abstract Objective: This study aimed to perform a meta-analysis to investigate the diagnostic safety and accuracy of Ultrasound-Guided Core Needle Biopsy (US-CNB) Axillary Lymph Nodes (ALNs) region in patients with Breast Cancer (BC). Methods: The authors searched the electronic databases PubMed, Scopus, Embase, and Web of Science for clinical trials about US-CNB for the detection of ALNs in breast cancer patients. The authors extracted and pooled raw data from the included studies and performed statistical analyses using Meta-DiSc 1.4 and Review Manager 5.3 software. A random effects model was used to calculate the data. At the same time, data from the Ultrasound-guided Fine-Needle Aspiration (US-FNA) were introduced for comparison with the US-CNB. In addition, the subgroup was performed to explore the causes of heterogeneity. (PROSPERO ID: CRD42022369491). Results: In total, 18 articles with 2521 patients were assessed as meeting the study criteria. The overall sensitivity was 0.90 (95% CI [Confidence Interval], 0.87‒0.91; p = 0.00), the overall specificity was 0.99 (95% CI 0.98‒ 1.00; p = 0.62), the overall area under the curve (AUC) was 0.98. Next, in the comparison of US-CNB and US-FNA, US-CNB is better than US-FNA in the diagnosis of ALNs metastases. The sensitivity was 0.88 (95% CI 0.84‒ 0.91; p = 0.12) vs. 0.73 (95% CI 0.69‒0.76; p = 0.91), the specificity was 1.00 (95% CI 0.99‒1.00; p = 1.00) vs. 0.99 (95% CI 0.67‒0.74; p = 0.92), and the AUC was 0.99 vs. 0.98. Subgroup analysis showed that heterogeneity may be related to preoperative Neoadjuvant Chemotherapy (NAC) treatment, region, size of tumor diameter, and the number of punctures. Conclusion: US-CNB has a satisfactory diagnostic performance with good specificity and sensitivity in the preoperative diagnosis of ALNs in BC patients. Objective metaanalysis meta UltrasoundGuided Ultrasound Guided USCNB US CNB (US-CNB (ALNs BC. . (BC) Methods PubMed Scopus Embase MetaDiSc Meta DiSc 14 1 4 1. 53 5 3 5. software time Ultrasoundguided guided FineNeedle Fine USFNA FNA (US-FNA USCNB. CNB. addition PROSPERO ID CRD42022369491. CRD42022369491 CRD CRD42022369491) Results total 252 criteria 090 0 90 0.9 95% 95 (95 Confidence Interval, Interval , Interval] 0.87‒0.91 087091 87 91 0.00, 000 0.00 00 0.00) 099 99 098 98 0.98 100 0.62, 062 0.62 62 0.62) (AUC Next USFNA, FNA, metastases 088 88 0.8 084 84 0.84 0.91 091 0.12 012 12 vs 073 73 0.7 0.69‒0.76 069076 69 76 0.91, 0.91) 1.0 0.99‒1.00 099100 0.67‒0.74 067074 67 74 0.92, 092 0.92 92 0.92) NAC (NAC treatment diameter punctures Conclusion (BC CRD4202236949 25 09 9 0. (9 0.87‒0.9 08709 8 0.0 10 06 0.6 6 08 0.1 01 07 7 0.69‒0.7 06907 0.99‒1.0 09910 0.67‒0.7 06707 CRD420223694 2 ( 0.87‒0. 0870 0.69‒0. 0690 0.99‒1. 0991 0.67‒0. 0670 CRD42022369 0.87‒0 087 0.69‒0 069 0.99‒1 0.67‒0 067 CRD4202236 0.87‒ 0.69‒ 0.99‒ 0.67‒ CRD420223 0.87 0.69 0.67 CRD42022 CRD4202 CRD420 CRD42 CRD4

ABSTRACT Objective: To explore the factors affecting short-term prognosis of circulatory failure patients undergoing venoarterial extracorporeal membrane oxygenation (VA-ECMO) treatment. Methods: A total of 136 patients undergoing VA-ECMO were enrolled in this study and subsequently divided into the death group (n=35) and the survival group (n=101) based on whether death occurred during hospitalisation. Extracorporeal membrane oxygenation (ECMO) running time, length of intensive care unit stay, length of hospital stay, costs, and ECMO complications were then compared between the two groups. Results: The average age of all patients undergoing ECMO was 47.64±16.78 years (53.2±16.20 years in the death group and 45.713±16.62 years in the survival group) (P=0.022). Patients in the survival group exhibited a clear downward trend in lactic acid value following ECMO treatment compared to those in the death group. Total hospitalisation stay was longer in the survival group (35 days) than in the death group (15.5 days) (P<0.001). In the analysis of ECMO complications, the incidence of neurological complications, renal failure, limb complications, and infection were higher in the death group than in the survival group (P<0.05 for all). Specifically, as a risk factor for patient survival and discharge, the occurrence of infection will lead to increased hospitalisation stays and costs (P<0.05 for both). Conclusion: Complications such as kidney failure and infection are associated with in-hospital death, and ECMO-related complications should be actively prevented to improve the survival rate of VA-ECMO treatment. Objective shortterm short term VAECMO VA (VA-ECMO Methods 13 n=35 n35 n 35 (n=35 n=101 n101 101 (n=101 (ECMO time groups Results 47641678 47 64 16 78 47.64±16.7 53.2±16.20 5321620 53 2 20 (53.2±16.2 457131662 45 713 62 45.713±16.6 P=0.022. P0022 P P=0.022 . 0 022 (P=0.022) (3 days 15.5 155 15 5 (15. P<0.001. P0001 P<0.001 001 (P<0.001) P<0.05 P005 05 (P<0.0 all. all) Specifically discharge both. both both) Conclusion inhospital ECMOrelated related 1 n=3 n3 3 (n=3 n=10 n10 10 (n=10 4764167 4 6 7 47.64±16. 53.2±16.2 532162 (53.2±16. 45713166 71 45.713±16. P002 P=0.02 02 (P=0.022 ( 15. (15 P000 P<0.00 00 (P<0.001 P<0.0 P00 (P<0. n= (n= n=1 n1 (n=1 476416 47.64±16 53.2±16. 53216 (53.2±16 4571316 45.713±16 P=0.0 (P=0.02 (1 (P<0.00 P<0. P0 (P<0 (n 47641 47.64±1 53.2±16 5321 (53.2±1 457131 45.713±1 P=0. (P=0.0 P<0 (P< 4764 47.64± 53.2±1 532 (53.2± 45713 45.713± P=0 (P=0. P< (P 476 47.64 53.2± (53.2 4571 45.713 P= (P=0 47.6 53.2 (53. 457 45.71 (P= 47. 53. (53 45.7 (5 45.

Abstract Objectives To investigate the changes in the coagulation function and hemodynamic parameters in patients with Hemorrhagic Traumatic Shock (HTS) after restrictive fluid resuscitation. Methods A total of 139 patients with HTS admitted to our hospital were enrolled, among which 69 HTS patients were divided into the control group and the remaining 70 HTS patients as the observation group. Patients in the control group underwent regular fluid resuscitation, while those in the observation group underwent restrictive fluid resuscitation. Results During treatment, 70 patients in the observation group had a lower bleeding amount, infusion amount, and blood transfusion volume than those in the control group (p < 0.05). After treatment, patients in the observation group had better hemodynamic parameters and blood coagulation than those in the control group (p < 0.05), and the incidence rate in the observation group was only 12.9%, which was significantly lower than 60.87% in the control group, while the cure rate in the observation group was 100%, which was significantly higher than that in the control group (p < 0.05). Conclusions Restrictive fluid resuscitation could remarkably increase the cure rate and reduce the bleeding amount during HTS treatment, thereby benefiting the recovery of the patient's blood coagulation. (HTS 13 enrolled 6 7 treatment p 0.05. 005 0.05 . 0 05 0.05) 0.05, , 129 12 9 12.9% 6087 60 87 60.87 100 100% patient s 1 00 0.0 12.9 608 8 60.8 10 0. 12. 60.

Abstract Under the influence of the COVID-19, people's awareness of physical health and immunity has increased significantly. Chitooligosaccharide is an oligomer of β-(1, 4)-linked D -glucosamine, furthermore, is one of the most widely studied immunomodulators. Chitooligosaccharide can be prepared from the chitin or chitosan polymers through enzymatically, chemically or physically processes. Chitooligosaccharide and its derivatives have been proven to have a wide range of biological activities including intestinal flora regulation, immunostimulant, anti-tumor, anti-obesity and anti-oxidation effects. This review summarizes the latest research of the preparation methods, biological activities in immunity and safety profiles of Chitooligosaccharide and its derivatives. We recapped the effect mechanisms of Chitooligosaccharide basing on overall immunity. Comparing the effects of Chitooligosaccharide with different molecular weights and degree of aggregation, a reference range for usage has been provided. This may provide a support for the application of Chitooligosaccharide in immune supplements and food. In addition, future research directions are also discussed. COVID19, COVID19 COVID 19, 19 COVID-19 peoples people s significantly β1, β1 β 1, 1 β-(1 4linked linked 4 glucosamine, glucosamine -glucosamine furthermore immunomodulators enzymatically processes regulation immunostimulant antitumor, antitumor anti tumor, tumor anti-tumor antiobesity obesity antioxidation oxidation methods aggregation provided food addition discussed COVID1 COVID-1 β-( COVID- β-

Abstract Background Alpha-kinase 1 (ALPK1) is a master regulator in inflammation and has been proved to promote renal fibrosis by promoting the production of IL-1β in diabetic nephropathy (DN) mice. Pyroptosis is involved in high glucose (HG)-induced tubular cells injury, characterized by activation of Gasdermin D (GSDMD) and the release of IL-1β and IL-18, resulting in inflammatory injury in DN. It is reasonable to assume that ALPK1 is involved in pyroptosis-related tubular injury in DN. However, the mechanism remains poorly defined. Methods Immunohistochemistry (IHC) staining was performed to detect the expression of pyroptosis- and fibrosis-related proteins in renal sections of DN patients and DN mice. DN models were induced through injection of streptozotocin combined with a high-fat diet. Protein levels of ALPK1, NF-κB, Caspase-1, GSDMD, IL-1β, IL-18 and α-SMA were detected by Western blot. HK-2 cells treated with high-glucose (HG) served as an in vitro model. ALPK1 small interfering RNA (siRNA) was transfected into HK-2 cells to down-regulate ALPK1. The pyroptosis rates were determined by flow cytometry. The concentrations of IL-1β and IL-18 were evaluated by ELISA kits. Immunofluorescence staining was used to observe translocation of NF-κB and GSDMD. Results The heat map of differentially expressed genes showed that ALPK1, Caspase-1 and GSDMD were upregulated in the DN group. The expression levels of ALPK1, Caspase-1, GSDMD and CD68 were increased in renal biopsy tissues of DN patients by IHC. ALPK1expression and CD68+ macrophages were positively correlated with tubular injury in DN patients. Western blot analysis showed increased expressions of ALPK1, phospho-NF-κB P65, GSDMD-NT, and IL-1β in renal tissues of DN mice and HK-2 cells, accompanied with increased renal fibrosis-related proteins (FN, α-SMA) and macrophages infiltration in interstitial areas. Inhibition of ALPK1 attenuated HG-induced upregulation expressions of NF-κB, pyroptosis-related proteins Caspase-1, GSDMD-NT, IL-1β, IL-18, α-SMA, and pyroptosis level in HK-2 cells. Also, the intensity and nuclear translocation of NF-κB and membranous translocation of GSDMD were ameliorated in HG-treated HK-2 cells after treatment with ALPK1 siRNA. Conclusions Our data suggest that ALPK1/NF-κB pathway initiated canonical caspase-1-GSDMD pyroptosis pathway, resulting in tubular injury and interstitial inflammation of DN.

RESUMO Objetivo: O futebol é o esporte preferido pelos estudantes universitários masculinos entre muitos esportes na China. Métodos: De acordo com a virilidade e as características competitivas dos estudantes universitários masculinos, um curso eletivo de futebol pode exercitar seus corpos e melhorar sua qualidade física. Embora os cursos eletivos afetem positivamente a saúde física dos estudantes universitários, diferentes projetos têm funções diferentes devido a seus outros conteúdos didáticos, formas de ensino, material didático, e métodos de ensino. Resultados: a implementação dos padrões nacionais da China para o físico dos estudantes universitários apresenta novas exigências para o nível de aptidão física dos estudantes universitários masculinos. No entanto, a pesquisa mostra que o nível geral de aptidão física dos estudantes universitários chineses está diminuindo, e a intensidade de exercício da maioria dos estudantes não pode atender às exigências. Conclusões: Para estudar a influência do esporte sobre o físico e a personalidade dos estudantes de acordo com o Student Physique and Health Standard, este documento estuda e analisa o impacto dos cursos eletivos de futebol universitário no corpo e na personalidade dos estudantes universitários e discute suas estratégias de treinamento. Nível de evidência II; Estudos terapêuticos – investigação de resultados de tratamento.

ABSTRACT Objective: Soccer is a favored sport for male college students among many sports in China. Methods: According to male college students’ virility and competitive characteristics, an elective soccer course can exercise their bodies and improve their physical quality. Although elective courses positively affect college students’ physical health, different projects have different functions due to their other teaching content, teaching forms, teaching material, and teaching methods. Results: the implementation of China’s national standards for college students’ physique presents new requirements for the fitness level of male college students. However, the research shows that the overall fitness level of Chinese college students is decreasing, and the exercise intensity of most students cannot meet the requirements. Conclusions: To study the influence of sports on students’ physique and personality according to the Student Physique and Health Standard, this paper studies and analyzes the impact of college soccer elective courses on college students’ bodies and personalities and discusses their training strategies. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMEN Objetivo: El fútbol es uno de los deportes favoritos de los estudiantes universitarios masculinos entre los muchos deportes que se practican en China. Métodos: De acuerdo con la virilidad y las características competitivas de los estudiantes universitarios masculinos, un curso de fútbol optativo puede ejercitar su cuerpo y mejorar su calidad física. Aunque los cursos optativos afectan positivamente a la salud física de los estudiantes universitarios, los distintos proyectos tienen funciones diferentes debido a otros contenidos didácticos, formas de enseñanza, material didáctico y métodos de enseñanza. Resultados: la aplicación de las normas nacionales chinas para el físico de los estudiantes universitarios presenta nuevos requisitos para el nivel de condición física de los estudiantes universitarios varones. Sin embargo, la investigación muestra que el nivel general de forma física de los estudiantes universitarios chinos está disminuyendo, y la intensidad del ejercicio de la mayoría de los estudiantes no puede cumplir con los requisitos. Conclusiones: Con el fin de estudiar la influencia del deporte en el físico y en la personalidad de los estudiantes de acuerdo con el Student Physique and Health Standard, este artículo estudia y analiza el impacto de los cursos optativos de fútbol universitario en el cuerpo y la personalidad de los estudiantes universitarios y discute sus estrategias de entrenamiento. Nivel de evidencia II; Estudios terapéuticos – investigación de resultados de tratamiento.

Abstract Coronary heart disease (CHD) is one of the main diseases causing morbidity and mortality globally, with oxidative damage and lipid peroxidation associated with oxidative stress and atherosclerosis. Cinnamomum migao is a Miao ethnomedicine that has been shown to provide relief from CHD symptoms. A gas chromatograph-mass spectrometer (GC-MS) was used to determine the chemical components of ten batches of migao fatty oil (MFO), yielding a total of 35 chemical constituents. The antioxidant activity of MFO in vitro was assessed using the DPPH· and ABTS·+ assay methods, and both indicated good antioxidant activity. The network pharmacology predicted the active constituents of MFO and their therapeutic influence on the mechanism of oxidative damage-induced coronary heart disease (OD-CHD). Six compounds were discovered in MFO, including tau-cadinol acetate and dodecanoic acid, which may play a role in OD-CHD by acting on lipids and atherosclerosis, the PI3K-Akt signalling pathway, and other pathways. In conclusion, our study lays the foundation for investigating the use of MFO in the treatment of OD-CHD.

RESUMEN La alta concentración de sal de cromo ha causado una grave contaminación al medio ambiente desde su producción. El residuo de cromo de larga data ha contaminado el suelo y la concentración total de cromo de algunos suelos contaminados ha alcanzado los 30000 mg / kg. Para la remediación de suelos contaminados con cromo se propuso el método electrodinámico mejorado. Para mejorar la eficiencia de la remediación electrocinética de suelos contaminados con cromo se propusieron dos tecnologías mejoradas de remediación electrocinética: Tecnología de remediación mejorada por oxidación electrocinética y tecnología de remediación mejorada por oxidación electrocinética. Cr (III) en el suelo fue contaminado por óxido de cromo oxidante para aumentar el contenido de Cr(VI) disuelto, para mejorar la eficiencia de la reparación eléctrica, para encontrar un medio PRB adecuado como un gran avance para mejorar la eficiencia de la reparación. Los resultados experimentales muestran que, en comparación con la tecnología electrodinámica tradicional, el método electrodinámico mejorado puede mejorar eficazmente la tasa de eliminación del cromo total en el suelo y proporcionar apoyo técnico para la remediación electrodinámica mejorada del suelo contaminado con cromo. El sistema de control de pH hace que el pH del electrolito del ánodo y del cátodo siempre se mantenga ácido, y la velocidad de migración del H+ es más rápida que la del OH-, y el pH del suelo cerca del ánodo es más bajo que el del cátodo.

ABSTRACT High concentration of chromium salt has caused serious pollution to the environment since its production. The long-standing chromium residue has polluted the soil, and the total chromium concentration of some polluted soil has reached 30000 mg / kg. For the remediation of chromium contaminated soil, the enhanced electrodynamic method was proposed. In order to improve the efficiency of electrokinetic remediation of chromium contaminated soil, two enhanced electrokinetic remediation technologies were proposed: Electrokinetic oxidation enhanced remediation technology and electrokinetic enhanced remediation technology. Cr(III) in soil was polluted by oxidant chromium oxide to increase the content of dissolved Cr(VI),so as to improve the efficiency of electric repair, in order to find a suitable PRB medium as a breakthrough to improve the repair efficiency. The experimental results show that compared with the traditional electrodynamic technology, the enhanced electrodynamic method can effectively improve the removal rate of total chromium in the soil, and provide technical support for the enhanced electrodynamic remediation of chromium contaminated soil. The pH control system makes the pH of anode and cathode electrolyte always maintain acidic, and the H+ migration speed is faster than that of OH-, and the pH of soil near the anode is lower than that near the cathode.

The etiology of polycystic ovary syndrome (PCOS) is complex and the pathogenesis is not fully understood. Some studies have shown that dysregulation of ovarian granulosa cells may be related to abnormal follicles and excessive androgen in women with PCOS. Our team has also confirmed the high expression status of H19 in PCOS patients in the early stage. However, the relationship between H19 and miR-19b in the development of PCOS is still unknown. Therefore, we used bioinformatics to predict the binding sites of human H19 and miR-19b, and of miR-19b and CTGF genes. After the silencing and overexpression of H19, real-time polymerase chain reaction (PCR) was used to detect the expressions of H19, miR-19b, and CTGF. Western blotting was used to detect CTGF protein. Proliferation of KGN cells after H19 silencing was detected by CCK8. Flow cytometry was used to detect the apoptosis of KGN cells after H19 silencing. After the overexpression of H19, it was found that the expression of miR-19b gene decreased and the expression of CTGF increased, whereas silencing of H19 did the opposite. In addition, H19 could promote cell proliferation and decrease cell apoptosis. Finally, luciferase reporter assays showed that the 3′-end sequences of lncRNA H19 and CTGF contained the binding site of miR-19b. In conclusion, our study indicated that lncRNA H19 acted as a ceRNA to bind to miR-19b via a “sponge” to regulate the effect of CTGF on KGN cells, which may play a vital role in PCOS.

SUMMARY: Matrigel is a basement membrane matrix extracted from the EHS mouse tumor containing extracellular matrix protein, its main components are laminin, type IV collagen, nestin, heparin sulfate, growth factor and matrix metalloproteinase.At room temperature, Matrigel polymerized to form a three dimensional matrix with biological activity. It can simulate the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of the cells in vitro, and can be used for the study of cell morphology, biochemical function, migration, infection and gene expression. In this study, Matrigel three-dimensional culture model of bone marrow mesenchymal stem cells(BMSCs) was established, and its morphology, proliferation and survival were observed. BMSCs were isolated and cultured with whole bone marrow adherence method. The Second generation BMSCs with good growth condition were selected and mixed with Matrigel to form cell gel complexes. The morphology and proliferation of mesenchymal stem cells were observed by phase contrast microscope and HE staining,Live/Dead staining was used to evaluate the cell activity.Phase contrast microscopy showed that BMSCs were reticulated in Matrigel and proliferated well, After 7 days, the matrix gel gradually became soft and collapsed, a few cell reticular crosslinking growth was seen at 14 days; HE staining showed that the cytoplasm of the cells was larger on the fourth day and the cells were elongated and cross-linked on the seventh day; Live/dead staining showed that most cells showed green fluorescence with the prolongation of culture time, on the first, 4 and 7 days, the activity of bone marrow mesenchymal stem cells in Matrigel gradually increased, and the percentages were 92.57 %, 95.54 % and 97.37 %, respectively. Matrigel three-dimensional culture system can maintain the morphology, function and proliferation ability of bone marrow mesenchymal stem cells.

RESUMEN: Matrigel es una matriz de membrana basal extraída del tumor de ratón EHS que contiene proteína de matriz extracelular. Los componentes principales son laminina, el colágeno tipo IV, nestina, sulfato de heparina, factor de crecimiento y metaloproteinasa de matriz. A temperatura ambiente, Matrigel se polimerizó para formar una matriz tridimensional. Es posible simular la estructura, la composición, las propiedades físicas y las funciones de la membrana basal celular in vivo, lo que es beneficioso para el cultivo y la diferenciación de las células in vitro, y se puede utilizar para el estudio de la morfología celular, la función bioquímica, la migración, infección y expresión génica. En este estudio, se estableció el modelo de cultivo tridimensional Matrigel de células madre mesenquimales de médula ósea (BMSC), y se observó su morfología, proliferación y supervivencia. Las BMSC fueron aisladas y cultivadas con el método de adherencia de la médula ósea completa. Se seleccionaron las BMSC de segunda generación con buenas condiciones de crecimiento y se mezclaron con Matrigel para formar complejos de gel de células. La morfología y la proliferación de las células madre mesenquimales se observaron con microscopio de contraste de fase y se tiñó con Hematoxilina-Eosina (HE); para evaluar la actividad celular se usó la tinción Live/Dead. La microscopía de contraste mostró que las BMSC se reticularon en Matrigel y proliferaron bien. Después de 7 días, se observó que el gel de matriz gradualmente se volvió blando y colapsó, y se visualizó un cruce transversal de algunas células reticulares a los 14 días. La tinción mostró que la mayoría de las células mostraron una fluorescencia verde con la prolongación del tiempo de cultivo; en los primeros 4 y 7 días, la actividad de las células madre mesenquimales de la médula ósea en Matrigel aumentó gradualmente y los porcentajes fueron de 92,57 %, 95,54 % y 97,37 %, respectivamente. El sistema de cultivo tridimensional de Matrigel puede mantener la morfología, la función y la capacidad de proliferación de las células madre mesenquimales de la médula ósea.