Abstract Objective: to determine the validity of the Global Leadership Initiative on Malnutrition (GLIM) against the Patient Generated-Subjective Global Assessment (PG-SGA) as a gold standard tool in malnutrition diagnosis, and to assess the impact of malnutrition diagnosed using GLIM and PG-SGA on the clinical outcomes of patients with esophageal squamous carcinoma (ESCC) resection. Methods: we prospectively analyzed 182 patients with ESCC who underwent radical esophagectomy at Zhongshan Hospital, Xiamen University, between October 2018 and December 2019. Preoperative malnutrition was diagnosed using GLIM and PG-SGA, and the postoperative clinical outcomes, including postoperative complications, postoperative chest tube indwelling time, length of stay and total hospitalization cost, were recorded. The association between the prevalence of malnutrition defined by the two tools and postoperative clinical outcomes was evaluated. Results: among the 182 ESCC patients, the incidence of malnutrition before surgery was 58.2 % and 48.4 % defined by PG-SGA and GLIM, respectively. GLIM and PG-SGA had good consistency in nutritional assessment of ESCC patients (k = 0.628, p < 0.001). Malnourished patients had higher TNM stages and older ages (all p < 0.05). Patients with malnutrition as assessed by PG-SGA and GLIM had a higher incidence of postoperative complications, a longer indwelling time of chest tube after esophagectomy, longer hospital length of stay, and higher hospitalization costs than patients with good nutrition (p < 0.001). Comparing the predictive efficiency of postoperative complications, the sensitivity of PG-SGA- and GLIM-defined malnutrition were 81.6 % and 79.6 %, the specificity were 50.4 % and 63.2 %, the Youden index were 0.320 and 0.428, and the Kappa value were 0.110 and 0.130, respectively. The areas under ROC curve of PG-SGA- and GLIM-defined malnutrition and postoperative complications were 0.660 and 0.714, respectively. Conclusions: this study indicates the effectiveness of malnutrition diagnosed according to GLIM and PG-SGA in predicting postoperative clinical outcomes among patients with ESCC. Compared with PG-SGA, GLIM criteria can better predict postoperative complications of ESCC. Follow-up analysis of postoperative long-term survival is needed to explore the association between different assessment tools and postoperative long-term clinical outcomes.

Resumen Objetivo: determinar la validez de la iniciativa de Liderazgo Global sobre la Malnutrición (GLIM) frente a la Evaluación Global Subjetiva Generada por el Paciente (PG-SGA) como herramienta de referencia en el diagnóstico de la malnutrición y evaluar el impacto de la malnutrición diagnosticada usando GLIM y PG-SGA en los resultados clínicos de los pacientes con resección de carcinoma escamoso de esófago (CEE). Métodos: se analizaron prospectivamente 182 pacientes con CEE sometidos a esofagectomía radical en el Hospital Zhongshan, de la Universidad de Xiamen, entre octubre de 2018 y diciembre de 2019. La desnutrición preoperatoria se diagnosticó utilizando GLIM y PG-SGA, y se registraron los resultados clínicos posoperatorios, incluyendo complicaciones posoperatorias, tiempo de permanencia del tubo torácico, posoperatorio, duración de la estancia y coste total de hospital. Se evaluó la asociación entre la prevalencia de desnutrición definida por las dos herramientas y los resultados clínicos posoperatorios. Resultados: entre 182 pacientes con CEE, la incidencia de desnutrición antes de la cirugía fue del 58,2 % y 48,4 % definida por PG-SGA y GLIM, respectivamente. GLIM y PG-SGA tuvieron buena consistencia en la evaluación nutricional de los pacientes con CEE (k = 0,628, p < 0,001). Los pacientes desnutridos presentaron estadios TNM más altos y edades mayores (todos p < 0,05). Los pacientes con desnutrición evaluada por PG-SGA y GLIM tuvieron una mayor incidencia de complicaciones posoperatorias, mayor tiempo de permanencia del tubo torácico después de la esofagectomía, mayor tiempo de hospitalización y mayores costos de hospitalización que los pacientes con buena nutrición (p < 0,001). Comparando la eficacia predictiva de las complicaciones posoperatorias, la sensibilidad de la desnutrición definida por PG-SGA y GPG fue del 81,6 % y 79,6 %; la especificidad, del 50,4 % y 63,2 %; el índice de Youden, del 0,320 y 0,428; y el valor de Kappa, de 0,110 y 0,130, respectivamente. Las áreas bajo la curva de ROC de la malnutrición definida por PG-SGA y GPG y las complicaciones postoperatorio fueron 0,660 y 0,714, respectivamente. Conclusiones: este estudio indica la eficacia de la desnutrición diagnosticada según GLIM y PG-SGA en la predicción de los resultados clínicos postoperatorios en pacientes con CEE. En comparación con PG-SGA, los criterios GLIM pueden predecir mejor las complicaciones posoperatorias del CEE. Es necesario realizar un análisis de seguimiento de la supervivencia posoperatoria a largo plazo para explorar la asociación entre las diferentes herramientas de evaluación y los resultados clínicos posoperatorios a largo plazo.

The reaction of metal hexafluoroacetylacetonato [MII(hfac)2, M = Cu, Mn] with the stable nitronyl nitroxide 2-(3-isobutyl-pyrazole)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (L), resulted in one dimensional zig-zag chain systems [{Cu(hfac)2}2L2]n and [CuMn(hfac)4L2]n. The main feature inherent in the nature of [{Cu(hfac)2}2L2]n single crystals is their ability to undergo reversible structural rearrangements with temperature variation, accompanied by anomalies of magnetism. The value of χmT shown strongly antiferromagnetic at low temperature and becomes ferromagnetic when the temperature increases. And the heteronuclear complex [CuMn(hfac)4L2]n shows antiferromagnetic interactions between manganese and nitronyl nitroxide. MIIhfac2, MIIhfac2 MIIhfac MII hfac 2, 2 [MII(hfac)2 Cu Mn 23isobutylpyrazole4,4,5,5tetramethylimidazoline1oxyl3oxide 23isobutylpyrazole4455tetramethylimidazoline1oxyl3oxide isobutylpyrazoletetramethylimidazolineoxyloxide 3 isobutyl pyrazole 4,4,5,5 tetramethylimidazoline 1 oxyl oxide 4 5 L, L , (L) zigzag zig zag Cuhfac22L2n CuhfacLn 2L2 n CuMnhfac4L2n. CuMnhfac4L2n CuMnhfacLn CuMn 4L2 n. variation magnetism increases [MII(hfac) 23isobutylpyrazole4 5tetramethylimidazoline1oxyl3oxide isobutylpyrazole 4455 4,4,5, (L Cuhfac 2L CuMnhfac 4L [MII(hfac 23isobutylpyrazole tetramethylimidazolineoxyloxide 445 4,4,5 44 4,4, 4,4 4,

Abstract Due to the good functional characteristics and nutritional value, ovalbumin (OVA) has a widely ranges of applications in food industry. But the characteristics of being susceptible to external conditions, such as processing, storage conditions and food ingredients, makes it display a relative lower functional property and limits its applications. Glycosylation is a natural, green approach of protein modifications that has an impact on its functional properties. In this work, we prepared the OVA-pectin conjugates through wet-heating and ultrasound glycosylation to investigate the effects on the functional and structural properties of OVA. Both wet-heating and ultrasound treated OVA at present of pectin showed higher emulsify and foam properties than untreated OVA. The results of particle size, SDS-PAGE, FT-IR, and SEM indicated that the covalent binding of pectin with OVA molecule. This study suggests that wet-heating and ultrasound glycosylation with pectin are promising method to improve the functional properties of OVA.

Abstract Purpose: To investigate the relations of neuropeptide Y (NPY) and heme oxygenase-1 (HO-1) expressions with fetal brain injury in rats with intrahepatic cholestasis of pregnancy (ICP). Methods: Sixty rats pregnant for 15 days were randomly divided into experimental and control groups. The ICP model was established in experimental group. On the 21st day, the blood biochemical test, histopathological examination of pregnant rat liver and fetal brain tissues and immunohistochemical analysis of fetal rat brain tissues were performed. Results: On the 21st day, the alanineaminotransferase, aspartate aminotransferase and total bile acid levels in experimental group were significantly higher than control group (P<0.01). Compared with control group, there was obvious vacuolar degeneration in pregnant rat liver tissue and fetal brain tissue in experimental group. NPY expression in fetal brain tissue was negative in control group and positive in experimental group. HO-1 expression in fetal brain tissue was strongly positive in control group and positive in experimental group. There was significant difference of immunohistochemical staining optical density between two groups (P<0.01). Conclusion: In fetal brain of ICP rats, the NPY expression is increased, and the HO-1 expression is decreased, which may be related to the fetal brain injury.

We constructed lentiviral vectors containing the human wild-type GJB6 gene and the mutant variants A88V and G11R. The three proteins were stably expressed by the Tet-on system in the HaCaT cell line and used to study the functional effect of the variants. The CCK-8 assay and flow cytometric analyses were used to determine the levels of cell proliferation and apoptosis. Western blot analyses were performed to analyze the relevant clinical indicators of hidrotic ectodermal dysplasia and markers of apoptosis in transfected HaCaT cells. The CCK8 assay and the flow cytometry results showed a significant increase (P<0.05) in the apoptosis of HaCaT cells expressing the A88V and G11R mutants. In addition, we demonstrated that the A88V and G11R mutants induced the apoptosis of transfected HaCaT cells via the activation of caspase-3, -8, -9, and PARA. No change was observed in the activity of BAX compared with the control. This study provides further clarification on the mechanisms underlying the effect of the mutant variants A88V and G11R of the GJB6 gene on the induction of HaCaT cell apoptosis.

Purpose To evaluate the efficacy and safety of onabotulinumtoxinA for patients with neurogenic detrusor overactivity (NDO). Materials and Methods We searched the Cochrane Library, PUBMED, EMBASE, Chinese Bio-medicine database, China Journal Full-text Database, VIP database, Wanfang database for randomized controlled trials (from inception to September 2012). Two authors independently selected studies, extracted data and assessed the methodological and evidence quality using the Cochrane Risk of Bias Table and GRADE (Grading of Recommendations, Assessment, Development and Evaluation) respectively. Data analysis was performed by RevMan 5.1 and descriptive analysis was employed if necessary. Results Eight studies were selected (n=1879 participants). OnabotulinumtoxinA was more related to urinary tract infection (UTI) (200U: OR 1.72, CI: 1.18-2.52; 300U: OR 1.88, CI: 1.31-2.69) versus placebo. Also, OnabotulinumtoxinA was superior to placebo in improving maximum cystometric capacity (MCC) (200U: OR 138.80, CI: 112.45-165.15; 300U: OR 152.09, CI: 125.25-178.93) and decreasing maximum detrusor pressure (MDP) (200U: MD -29.61, CI: -36.52--22.69; 300U: MD-28.92, CI: -39.59--18.25). However, there were no statistical differences between 200U and 300U onabotulinumtoxinA in UTI (OR 0.84, CI: 0.58-1.22), MCC (OR-12.72, CI: -43.36-17.92) and MDP (MD 2.21, CI: -6.80-11.22). Conclusions OnabotulinumtoxinA may provide superior clinical and urodynamic benefit for populations with NDO. High-quality studies are required for evaluating the optimal dose, long-term application and when to perform repeated injections.

Objective To compare the effectiveness of mobile phone text messaging and that of traditional health worker training in communicating clinical recommendations to health workers in China. Methods A cluster-randomized controlled trial (Chinese Clinical Trial Register: ChiCTR-TRC-09000488) was conducted in 100 township health centres in north-western China between 17 October and 25 December 2011. Health workers were allocated either to receive 16 text messages with recommendations on the management of viral infections affecting the upper respiratory tract and otitis media (intervention group, n = 490) or to receive the same recommendations through the existing continuing medical education programme – a one-day training workshop (control group, n = 487). Health workers’ knowledge of the recommendations was assessed before and after messaging and traditional training through a multiple choice questionnaire. The percentage change in score in the control group was compared with that in the intervention group. Changes in prescribing practices were also compared. Findings Health workers’ knowledge of the recommendations increased significantly in the intervention group, both individually (0.17 points; 95% confidence interval, CI: 0.168–0.172) and at the cluster level (0.16 points; 95% CI: 0.157–0.163), but not in the control group. In the intervention group steroid prescriptions decreased by 5 percentage points but antibiotic prescriptions remained unchanged. In the control group, however, antibiotic and steroid prescriptions increased by 17 and 11 percentage points, respectively. Conclusion Text messages can be effective for transmitting medical information and changing health workers' behaviour, particularly in resource-limited settings.

Resumen Objetivo Comparar la eficacia de los mensajes de texto por teléfono móvil y la de la formación tradicional del personal sanitario en la comunicación de las recomendaciones clínicas al personal sanitario en China. Métodos Se llevó a cabo un ensayo controlado aleatorio de grupos (registro chino de ensayos clínicos: ChiCTR-TRC-09000488) en 100 centros de salud de municipios situados en el noroeste de China entre el 17 de octubre y el 25 diciembre de 2011. Se determinó que el personal sanitario recibiría bien 16 mensajes de texto con recomendaciones sobre el tratamiento de las infecciones víricas que afectan a las vías respiratorias superiores y otitis media (grupo de intervención, n = 490) o bien las mismas recomendaciones a través del programa de educación médica continua existente - un taller de capacitación de un día de duración (grupo de control, n = 487). Los conocimientos del personal sanitario se evaluaron antes y después del envío de los mensajes de texto y de la formación tradicional por medio de un cuestionario de respuestas múltiples. Se comparó el porcentaje de cambio en la puntuación del grupo de control con el del grupo de intervención, así como los cambios en las prácticas de prescripción. Resultados El conocimiento del personal sanitario acerca de las recomendaciones aumentó significativamente en el grupo de intervención, tanto a nivel individual (0,17 puntos, IC del 95%: 0,168–0,172) como a nivel del grupo (0,16 puntos; intervalo de confianza del 95%: 0,157–0,163), pero no en el grupo de control. En el grupo de intervención, las prescripciones de esteroides disminuyeron en 5 puntos porcentuales, aunque las de antibióticos no presentaron cambios. En el grupo de control, sin embargo, aumentaron las prescripciones de esteroides y de antibióticos en, respectivamente, 17 y 11 puntos porcentuales. Conclusión Los mensajes de texto pueden ser eficaces para transmitir información médica y cambiar el comportamiento del personal sanitario, especialmente en entornos con recursos limitados.

Résumé Objectif Comparer l'efficacité du texting par téléphone portable et celle de la formation traditionnelle des professionnels de la santé en matière de communication des recommandations cliniques aux professionnels de la santé en Chine. Méthodes Un essai contrôlé randomisé par grappes (numéro d'enregistrement des essais cliniques chinois: ChiCTR-TRC-09000488) a été mené dans 100 centres de santé communaux dans le nord-ouest de la Chine entre le 17 octobre et le 25 décembre 2011. Les professionnels de la santé ont reçu soit 16 messages textuels contenant des recommandations sur la gestion des infections virales affectant les voies respiratoires supérieures et des otites moyennes (groupe d'intervention, n = 490), soit les mêmes recommandations, mais par le biais du programme de formation continue médicale existant – un atelier de formation d'une journée (groupe témoin, n = 487). Les connaissances des professionnels sur ces recommandations ont été évaluées avant et après la réception des messages textuels et la formation traditionnelle par le biais d'un questionnaire à choix multiples. La variation en pourcentage du score obtenu par le groupe témoin a été comparée à celle du groupe d'intervention. Les changements dans les pratiques de prescription ont également été comparés. Résultats Les connaissances des professionnels de la santé sur les recommandations ont augmenté significativement dans le groupe d'intervention, à la fois individuellement (0,17 point; intervalle de confiance de 95%, IC de 95%: 0,168–0,172) et au niveau de la grappe (0,16 point; IC de 95%: 0,157–0,163), mais pas dans le groupe témoin. Dans le groupe d'intervention, les prescriptions de stéroïdes ont diminué de 5 points de pourcentage, mais les prescriptions d'antibiotiques sont restées constantes. Dans le groupe témoin cependant, les prescriptions d'antibiotiques et de stéroïdes ont augmenté respectivement de 17 et 11 points de pourcentage. Conclusion Les messages textuels peuvent être efficaces pour transmettre des informations médicales et changer le comportement des professionnels de la santé, en particulier dans les endroits où les ressources sont limitées.

The impact of dietary 2-hydroxy-4-(methylthio) butyric acid isopropyl ester (HMBi) on rumen fermentation in cashmere goats was investigated to determine the effect of this ester on cashmere production. Four healthy castrated Liaoning cashmere goats with permanent rumen cannulas were assayed for four periods. In each period, goats were fed a different diet: control diet (containing 0% MetaSmart (an HMBi product)), diet I (containing 0.85% MetaSmart), diet II (containing 1.27% MetaSmart) or diet III (containing 1.70% MetaSmart). Rumen pH, ammonia-N concentration, volatile fatty acid (VFA) concentrations and total protein concentration were determined after feeding in each period. Results showed that rumen pH significantly increased and rumen ammonia-N concentration significantly decreased when the diet was supplemented with 0.85%, 1.27% and 1.70% MetaSmart. Diets with 1.27% and 1.70% MetaSmart increased rumen total VFA concentration, and 3 hours post feeding all three HMBi diets increased acetic acid concentration. Rumen total protein concentration significantly increased in the goats fed 1.27% or 1.70% MetaSmart. Thus, diets supplemented with HMBi significantly affected rumen pH, ammonia-N concentration, VFA concentrations and total protein content. Dietary HMBi can promote rumen fermentation in cashmere goats.