Abstract A novel wideband high-selectivity substrate-integratedwaveguide (SIW) filtering antenna with controllable radiation nulls is proposed. This antenna is composed of a two-layered SIW resonator, two radiating slots on the top layer, and two coupling slots on the middle layer. A vertical SMA connector is located at the bottom and feeds two SIW cavities simultaneously. By adjusting the parameters of these slots, three radiation nulls will be achieved, and two radiation nulls can be easily controlled. For demonstration, one prototype of the filtering antenna is fabricated and measured. Measurement results show that the fractional bandwidth for |S11| < -10 dB is 8.9% (3.86-4.23 GHz), the gain is 6.08 dBi at center frequency 4.05 GHz, three radiation nulls are achieved at 3.7 GHz, 4.3 GHz, and 5.19 GHz, respectively. The out-of-band skirt selectivities of two sidebands are 150 and 332 dB/GHz, respectively. highselectivity high selectivity substrateintegratedwaveguide substrate integratedwaveguide (SIW proposed twolayered layered resonator layer simultaneously controlled demonstration measured S11 S |S11 10 -1 89 8 9 8.9 3.864.23 386423 3.86 4.23 3 86 4 23 (3.86-4.2 GHz , GHz) 608 6 08 6.0 405 05 4.0 37 7 3. 43 4. 519 5 19 5.1 respectively outofband out band 15 33 dBGHz dB/GHz S1 |S1 1 - 8. 864 3.864.2 38642 386 3.8 423 4.2 2 (3.86-4. 60 0 6. 40 51 5. |S 3.864. 3864 38 42 (3.86-4 3.864 (3.86- (3.86 (3.8 (3. (3 (

Abstract Background: The influences of Oxycodone (OXY) combined with Paclitaxel (PTX) on breast cancer cells are unclear. The present study aimed to examine the effects of OXY combined with PTX on the proliferation, apoptosis, and migration of human breast cancer SKBR3 cells and the underlying mechanism. Methods: The proliferation, apoptosis and invasion of SKBR3 cells were assessed by CCK-8, colony formation assay, flowcytometric, Transwell assay and scratch assays, respectively. In addition, Western blotting was used to detect the expression of related proteins in these cells. The autophagic bodies were observed under a transmission electron microscope. Results: OXY (0.25, 0.5 and 1 mM) significantly inhibited the viability, colony-forming, migration, and invasion of SKBR3 cells as compared to the control group. Furthermore, OXY (0.25, 0.5 and 1 mM) markedly induced the apoptosis of SKBR3 cells and the levels of apoptosis-related proteins. In addition, OXY (0.25, 0.5 and 1 mM) and PTX inhibited the proliferation of SKBR3 cells synergistically as compared to PTX group in vitro. Moreover, OXY (0.25, 0.5 and 1 mM) significantly elevated the PTX-induced apoptosis in SKBR3 cells via downregulating the expression of N-cadherin, Becline-1 LC3-II, p-Akt and p-mTOR and upregulating E-cadherin expression. Compared with the control group, OXY (1 mM) treatment induced autophagy in SKBR3 cells. Conclusions: The present study indicates that OXY can enhance the antitumor effect of PTX on breast cancer in vitro. Hence, the combination of OXY with PTX may serve as a potential strategy for the treatment of breast cancer. Background (OXY (PTX unclear SKBR mechanism Methods CCK8, CCK8 CCK 8, 8 CCK-8 flowcytometric assays respectively addition microscope Results 0.25, 025 0 25 (0.25 05 5 0. mM viability colonyforming, colonyforming forming, forming colony-forming Furthermore apoptosisrelated vitro Moreover PTXinduced Ncadherin, Ncadherin N cadherin, cadherin N-cadherin Becline1 Becline Becline- LC3II, LC3II LCII LC3 II, II LC LC3-II pAkt p Akt pmTOR mTOR Ecadherin E ( Conclusions Hence CCK- 0.25 02 2 (0.2 0.2 (0. (0

Abstract RFX2 plays critical roles in mammalian spermatogenesis and cilium maturation. Here, the testes of 12-month-old adult boars of Banna mini-pig inbred line (BMI) were subjected to whole-transcriptome sequencing. The results indicated that the average expression (raw count) of RFX2 gene in BMI testes was 16138.25, and the average expression value of the corresponding transcript ENSSSCT00000043271.2 was 123.1898. The CDS of RFX2 obtained from BMI testes was 2,817 bp (GenBank accession number: OL362242). Gene structure analysis showed that RFX2 was located on chromosome 2 of the pig genome with 19 exons. Protein structure analysis indicated that RFX2 contains 728 amino acids with two conserved domains. Phylogenetic analysis revealed that RFX2 was highly conserved with evolutionary homologies among mammalian species. Other analyses, including PPI networks, KEGG, and GO, indicated that BMI RFX2 had interactions with 43 proteins involving various functions, such as in cell cycle, spermatid development, spermatid differentiation, cilium assembly, and cilium organization, etc. Correlation analysis between these proteins and the transcriptome data implied that RFX2 was significantly associated with FOXJ1, DNAH9, TMEM138, E2F7, and ATR, and particularly showed the highest correlation with ATR, demonstrating the importance of RFX2 and ART in spermatogenesis. Functional annotation implied that RFX2 was involved in 17 GO terms, including three cellular components (CC), six molecular functions (MF), and eight biological processes (BP). The analysis of miRNA-gene targeting indicated that BMI RFX2 was mainly regulated by two miRNAs, among which four lncRNAs and five lncRNAs competitively bound ssc-miR-365-5p and ssc-miR-744 with RFX2, respectively. Further, the dual-luciferase report assay indicated that the ssc-miR-365-5p and ssc-miR-744 significantly reduced luciferase activity of RFX2 3'UTR in the 293T cells, suggesting that these two miRNAs regulated the expression of RFX2. Our results revealed the important role of RFX2 in BMI spermatogenesis, making it an intriguing candidate for follow-up studies. RFX maturation Here 12monthold monthold 12 month old minipig mini (BMI wholetranscriptome whole sequencing raw count 1613825 16138 25 16138.25 ENSSSCT000000432712 ENSSSCT ENSSSCT00000043271 ENSSSCT00000043271. 1231898 123 1898 123.1898 2817 817 2,81 GenBank number OL362242. OL362242 OL . OL362242) 1 exons 72 domains species analyses networks KEGG 4 cycle development differentiation assembly organization etc FOXJ1 FOXJ DNAH9 DNAH TMEM138 TMEM E2F7 EF E F ATR terms CC, CC , (CC) MF, MF (MF) BP. BP (BP) miRNAgene miRNA sscmiR3655p sscmiRp ssc miR 365 5p p sscmiR744 sscmiR 744 ssc-miR-74 respectively Further dualluciferase dual 3UTR UTR 3 T cells followup follow up studies 161382 1613 16138.2 ENSSSCT0000004327 123189 189 123.189 281 81 2,8 OL36224 7 TMEM13 E2F (CC (MF (BP 36 sscmiR74 74 ssc-miR-7 161 16138. ENSSSCT000000432 12318 18 123.18 28 8 2, OL3622 TMEM1 sscmiR7 ssc-miR- 16 ENSSSCT00000043 1231 123.1 OL362 ssc-miR ENSSSCT0000004 123. OL36 ENSSSCT000000 OL3 ENSSSCT00000 ENSSSCT0000 ENSSSCT000 ENSSSCT00 ENSSSCT0

OBJECTIVES: To evaluate the efficacy of autologous platelet-rich plasma (PRP) gel in the treatment of refractory pressure injuries and its effect on wound healing time and quality of life of patients. METHODS: A random number table method was used to group 102 patients with refractory pressure injuries into either a control group (CG) (51 cases) receiving negative pressure wound therapy (NPWT) or a study group (SG) (51 cases) receiving NPWT+PRP gel. RESULTS: The total efficacy rate in the SG (92.16%) was higher than that in the CG (76.47%) (p<0.05). The SG exhibited lower visual analog scale (VAS) scores and pressure ulcer scale for healing (PUSH) scores, smaller wound sizes and depths, and shorter wound healing times than the CG after 21 days of treatment (p<0.05). After 6 months of treatment, the SG scored higher than the CG on the psychological, physiological, social functions, and daily activity domains on the World Health Organization Quality of Life (WHOQOL-BREF) scale (p<0.05). The incidence of postoperative complications in the SG (13.73%) was not significantly different from that of the CG (7.84%) (p>0.05). CONCLUSION: In the treatment of refractory pressure injuries, PRP gel can accelerate wound healing, reduce wound pain, shorten the treatment cycle, regulate tissue inhibitor matrix metalloproteinase-1 (TIMP-1) and matrix metalloproteinase-9 (MMP-9) levels and the expression of specific proteins in granulation tissue, reduce the levels of the inflammatory factors interleukin-1β (IL-1β), IL-8, and tumor necrosis factor-α (TNF-α), and improve the quality of life of patients without increasing complications.

The reaction mechanism of AgIII complex ([Ag(HIO6)2]5–) with ruthenium(II) bipyridine (Ru(bpy)32+) in acid medium was investigated. A novel flow injection chemiluminescence (CL) analysis method was developed for the detection of norfloxacin (NFLX) and ofloxacin (OFLX) in commercial drug, milk and human urine samples based on AgIII-ruthenium(II) bipyridine in acidic solution. Under optimal conditions, CL intensities were proportional to drug concentrations in the range of 1.6 × 10-9 to 8 × 10-6 g mL-1 for NFLX and 1.0 × 10-9 to 4 × 10-6 g mL-1 for OFLX. The detection limit was 1.0 × 10-9 g mL-1 for NFLX and 0.8 × 10-9 g mL-1 for OFLX. On the basis of the CL, UV and fluorescence spectra, possible reaction mechanisms were proposed.

Abstract The biodegradation of synthetic dyes by fungi is emerging as an effective and promising approach. In the present study, freshwater fungal strains isolated from submerged woods were screened for the decolorization of 7 synthetic dyes. Subsequently, 13 isolates with high decolorization capability were assessed in a liquid system; they belonged to 9 different fungal species. Several strains exhibited a highly effective decolorization of multiple types of dyes. New absorbance peaks appeared after the treatment with 3 fungal strains, which suggests that a biotransformation process occurred through fungal biodegradation. These results showed the unexploited and valuable capability of freshwater fungi for the treatment of dye-containing effluents. The ability of certain fungi to decolorize dyes is reported here for the first time.

Background: In recent years, obesity has become the major public health problems worldwide. The detection rate of obesity is alarmingly rising among children and adolescents in China, whose population of 120 million are in range of obesity. It was estimated that 8% of her children are obese. Methods: Publications between 2009 and 2014 on the obesity prevalence among primary school students in China were retrieved from PubMed, online Chinese periodical full-text databases of VIP, CNKI and Wanfang. Meta Analyst software was used to sum up and analyze the detection rates included in the previously retrieved literatures. Results: After evaluation of the quality of the articles, 18 papers were finally included in our study, and the total sample sizes on the obesity investigation were 247547, in which 26466 were obesity. Meta-analysis findings showed that the pooled prevalence of obesity in primary school students is 10.0 %( 95% CI: 7.2%-13.5%). Conclusion: Our results indicated that the obesity prevalence status in China was still troublesome, for the situation will go worse if we currently fail to take effective and practical measures.

Introducción: En los últimos años, la obesidad se ha convertido en el principal problema de salud pública a nivel mundial. La tasa de detección de obesidad infantil está creciendo alarmantemente entre los niños y los adolescentes en China, cuya población de 120 millones se sitúa en el rango de obesidad. Se calculó que el 8% de los niños son obesos. Métodos: Se recopilaron publicaciones entre 2009 y 2014 sobre la prevalencia de obesidad entre los alumnos de primaria en China de PubMed, textos completos de bases de datos de periódicos chinos online de VIP, CNKI y Wanfang. Se empleó software de meta-análisis para resumir y analizar las tasas de detección incluidas en la bibliografía previamente obtenida. Resultados: Después de la evaluación de la calidad de los artículos, finalmente incluimos 18 artículos en nuestro estudio, y los tamaños de la muestra total en la investigación sobre obesidad fueron 247547, donde 26466 presentaron obesidad. Los resultados de los meta-análisis mostraron que la prevalencia de la obesidad en los alumnos de primaria es del 10,0% (IC del 95%: 7,2% - 13,5%). Conclusión: Nuestros resultados indican que la prevalencia de obesidad en China todavía supone un problema preocupante, dado que la situación será peor si no se toman medidas eficaces y prácticas.

JUSTIFICATIVA E OBJETIVOS: Os anestésicos locais são amplamente utilizados na prevenção ou na reversão de dor aguda e no tratamento de dor crônica. A reação de cardiotoxicidade induzida pelos anestésicos locais é um evento acidental sem terapia farmacológica, exceto a infusão de intralípides relatados recentemente cujo mecanismo de ação ainda não é bem compreendido. CONTEÚDO: A cardiolipina, um fosfolipídio aniônico, desempenha papel relevante na determinação de reação respiratória mitocondrial, metabolismo de ácidos graxos e apoptose celular. A disfunção do metabolismo energético mitocondrial é sugerida em associação com a cardiotoxicidade dos anestésicos locais, a partir de um estudo in vitro de que ela talvez se deva a fortes ligações eletrostáticas entre os anestésicos locais e a cardiolipina na membrana mitocondrial. Não há, contudo, evidência experimental. Portanto, levantamos a hipótese de que as interações anestésico-cardiolipina sejam o principal determinante associado à reação de cardiotoxicidade, o que pode ser estabelecido com a adoção de métodos teóricos e biológicos estruturais. Esse modelo de interação nos daria uma pista sobre o mecanismo da cardiotoxicidade dos anestésicos locais, visando a futuras pesquisas na área de desenvolvimento de fármacos de prevenção a esse evento na prática clínica. CONCLUSÕES: A interação entre a cardiolipina mitocondrial e os anestésicos locais pode ser a principal fonte de sua cardiotoxicidade, em função de seus efeitos sobre o metabolismo energético e o estado eletrostático.

BACKGROUND AND OBJECTIVES: Local anesthetics are used broadly to prevent or reverse acute pain and treat symptoms of chronic pain. Local anesthetic-induced cardiotoxic reaction has been considered the accidental event without currently effective therapeutic drugs except for recently reported intralipid infusion whose possible mechanism of action is not well known. CONTENTS: Cardiolipin, an anionic phospholipid, plays a key role in determining mitochondrial respiratory reaction, fatty acid metabolism and cellular apoptosis. Mitochondrial energy metabolism dysfunction is suggested as associated with local anesthetic cardiotoxicity, from an in vitro study report that the local anesthetic cardiotoxicity may be due to the strong electrostatic interaction of local anesthetics and cardiolipin in the mitochondria membrane, although there is a lack for experimental evidence. Herein we hypothesized that local anesthetic-cardiolipin interactions were the major determinant of local anesthetic-associated cardiotoxic reaction, established by means of theoretic and structural biological methods. This interacting model would give an insight on the underlying mechanism of local anesthetic cardiotoxicity and provide clues for further in depth research on designing preventive drugs for such inadvertent accidence in routine clinical practice. CONCLUSIONS: The interaction between local anesthetic and mitochondrial cardiolipin may be the underlying mechanism for cardiotoxicity affecting its energy metabolism and electrostatic status.