ABSTRACT Objective: To evaluate the properties of fermented milk beverages containing probiotics and their potential demineralization on enamel after consumption. Material and Methods: Seven fermented milks were analyzed by titratable acidity and pH, and five were considered for erosive cycling. The beverages' calcium (Ca) and Phosphorus (P) were measured. For measurement of potential erosive, bovine dental enamel blocks (n=8) were individually treated (2 min - 4x/day for five days) with 1% citric acid solution pH 3.5 (control) and strawberry, grape, traditional, and orange fermented milk beverages. At treatment intervals, the blocks were immersed in artificial saliva (1h). The samples were evaluated by profilometry and scanning electron microscopy (SEM) at the end of cycling. The results were submitted to ANOVA and 5% Tukey tests. Results: For titratable acidity, the traditional flavor had the lowest value, 3.36 ± 0.46 mmoles, and the grape flavor had the highest value, 10.7 ± 0.1 mmoles. The lowest pH value was the strawberry flavor at 3.61 ± 0.07, and the highest was the orange flavor at 4.10 ± 0.07. The orange flavor showed the highest values of Ca (48.19%) and P (9.69%). The fermented milk beverages promoted surface loss; however, citric acid promoted higher values. Conclusion: Fermented milk is an acidic beverage with variations in inorganic components. Their erosive potential is lower compared with citric acid. Objective consumption Methods cycling (Ca (P measured n=8 n8 n 8 (n=8 2 ( 4xday xday 4x day x days 1 35 3 5 3. control (control intervals 1h. 1h h . (1h) SEM (SEM tests Results 336 36 3.3 046 0 46 0.4 mmoles 107 10 7 10. 01 0. 361 61 3.6 007 07 0.07 410 4 4.1 48.19% 4819 48 19 (48.19% 9.69%. 969 9.69% 9 69 (9.69%) loss however Conclusion components n= (n= (1h 33 04 6 00 0.0 41 4. 48.19 481 (48.19 96 9.69 (9.69% (n 48.1 (48.1 9.6 (9.69 48. (48. 9. (9.6 (48 (9. (4 (9

Resumo O objetivo deste trabalho foi determinar os níveis de acidez para obter os atributos químicos do solo adequados à máxima produção de frutos de amoreiras-pretas cultivadas em solos ácidos submetidos a doses crescentes de calcário. Foram avaliadas as cultivares de amoreira-preta BRS Tupy, Brazos, Guarani e BRS Xavante nos solos Latossolo Vermelho Distroférico (LVdf) e Latossolo Vermelho distrófico (LVd), aos quais foram aplicadas quatro doses de calcário dolomítico (0, 1,5, 3,0 e 6,0 Mg ha-1). Foram avaliados o efeito da calagem sobre os atributos químicos do solo, em arranjo fatorial 2×4 (solos e doses de calcário), bem como o teor de nutrientes foliares e a produção de frutos, em arranjo fatorial 2×4×4 (solos, doses de calcário e cultivares). A produção máxima de frutos foi obtida com 3,3 Mg ha-1 de calcário dolomítico. As cultivares de amoreira-preta apresentaram alta demanda por Ca, Mg e saturação por bases e baixa tolerância ao alumínio nos solos. A partir de 3,3 Mg ha-1 de calcário dolomítico, a produção máxima de frutos de amoras-pretas cultivadas em solos ácidos é obtida com pH 5,6, saturação máxima tolerada de alumínio de 6%, concentração de Ca e Mg de 45,3 mmolc kg-1 e saturação por bases de 48,3%. amoreiraspretas amoreiras pretas amoreirapreta amoreira preta Tupy Brazos LVdf (LVdf LVd, LVd , (LVd) 0, 0 (0 15 1 5 1,5 30 3 3, 60 6 6, ha1. ha1 ha . ha-1) 24 2 4 2× calcário, calcário) 244 2×4× solos, cultivares. cultivares) 33 ha- amoraspretas amoras 56 5,6 6% 453 45 45, kg1 kg kg- 483 48 48,3% (LVd ( 1, 5, 48,3 48,

Abstract The objective of this work was to determine the acidity levels to obtain adequate soil chemical attributes for the maximum fruit yield of blackberry trees grown in acidic soils subjected to increasing limestone rates. The BRS Tupy, Brazos, Guarani, and BRS Xavante blackberry cultivars were evaluated on the Typic Hapludox (LVdf) and Rhodic Hapludox (LVd) soils, to which four rates of dolomitic limestone were applied (0, 1.5, 3.0, and 6.0 Mg ha-1). The effect of liming on soil chemical attributes, in a 2×4 factorial arrangement (soils and limestone rates), as well as leaf nutrient content and fruit yield, in a 2×4×4 factorial arrangement (soils, limestone rates, and cultivars), were evaluated. The maximum fruit yield was obtained with 3.3 Mg ha-1 dolomitic limestone. The blackberry cultivars showed a high demand for Ca, Mg, and base saturation and a low tolerance to aluminum in the soils. From 3.3 Mg ha-1 dolomitic limestone, the maximum fruit yield of blackberry trees grown in acidic soils is obtained with pH 5.6, maximum tolerated aluminum saturation of 6%, Ca and Mg concentration of 45.3 mmolc kg-1, and base saturation of 48.3%. Tupy Brazos Guarani LVdf (LVdf LVd (LVd 0, 0 (0 15 1 5 1.5 30 3 3.0 60 6 6. ha1. ha1 ha . ha-1) 24 2 4 2× , rates) 244 2×4× cultivars, cultivars) 33 3. ha- 56 5.6 6% 453 45 45. kg1, kg1 kg 1, kg-1 483 48 48.3% ( 1. 5. kg- 48.3 48.

Abstract The larynx is in the lower respiratory tract and has the function of protecting the airways, controlling, and modulating breathing, assisting the circulatory system, and vocalizing. This study aims to describe the anatomy and histology of the skeleton of the larynx and trachea of the species Chelonia mydas, Caiman yacare and Caiman latirostris. The study was conducted at the Federal University of Espírito Santo (UFES), using nine specimens of Ch. mydas, 20 of Ca. yacare and four of Ca. latirostris. Samples of the larynx and trachea were collected, fixed, and sent for dissection of the structures and subsequent macroscopic analysis. For histology, samples were processed by the routine paraffin embedding method and stained with hematoxylin-eosin and Verhoeff. For the three species, two arytenoid cartilages, a cricoid cartilage, a hyoid apparatus composed of a base and two horns were found. In Ch. mydas, two structures called thyroid wings were observed, not found in crocodilians. The trachea of crocodilians presented incomplete tracheal rings and musculature, while the trachea of Ch. mydas presented complete tracheal rings. Histologically, the entire cartilaginous skeleton of the larynx of the three species, as well as the tracheal rings, are constituted by hyaline cartilage. airways controlling breathing system vocalizing latirostris UFES, UFES , (UFES) Ch 2 Ca collected fixed analysis hematoxylineosin hematoxylin eosin Verhoeff cartilages cartilage observed musculature Histologically (UFES

Abstract In Brazil, most of cereal production is concentrated in the Cerrado which is characterized by poor soils. Thus, the soil fertility management is important for suitable use of Cerrado’s soils. After 40 years of increasing use of Cerrado for agricultural purposes, this work aimed to evaluate the soil fertility of a representative area known as Triângulo Mineiro and Alto Paranaíba (TMAP) mesoregion. Different land uses from 126 farms were selected for soil sampling and assessed the following properties: pH; exchangeable Al3+, Ca2+, Mg2+; available P, K+, S, B, Fe, Cu, Mn, and Zn; soil organic matter (SOM); and cation exchange capacity (CEC) effective and potential at pH 7.0. Land uses improved the soil fertility properties compared to native Cerrado vegetation, except planted forests. The pH increased and Al3+ was neutralized, however, satisfactory contents of Ca2+ and Mg2+ were not reached. CEC is still considered low to medium, since SOM was not significantly increased. Available P, S, Fe, Mn, Zn, and Cu contents increased, being considered satisfactory. In general, our findings suggest that the nutrients can be considered adequate for crop production and sustainability. However, continuous monitoring for maintenance of soil fertility and adoption of best management practices are needed. Brazil soils Thus Cerrados s 4 purposes TMAP (TMAP mesoregion 12 Al3 Al Ca2 Ca Mg2 Mg P K K+ S B Fe Mn Zn (SOM) (CEC 70 7 0 7.0 vegetation forests neutralized however reached medium general sustainability However needed 1 (SOM 7.

Abstract Objectives: To identify somatic mutations in tumors from young women with triple-negative or luminal breast cancer, through targeted sequencing and to explore the cancer driver potential of these gene variants. Methods: A customized gene panel was assembled based on data from previous sequencing studies of breast cancer from young women. Triple-negative and luminal tumors and paired blood samples from young breast cancer patients were sequenced, and identified gene variants were searched for their driver potential, in databases and literature. Additionally, the authors performed an exploratory analysis using large, curated databases to evaluate the frequency of somatic mutations in this gene panel in tumors stratified by age groups (every 10 years). Results: A total of 28 young women had their tumoral tissue and blood samples sequenced. Using a customized panel of 64 genes, the authors could detect cancer drivers in 11/12 (91.7 %) TNBC samples and 11/16 (68.7 %) luminal samples. Among TNBC patients, the most frequent cancer driver was TP53, followed by NF1, NOTCH1 and PTPN13. In luminal samples, PIK3CA and GATA3 were the main cancer drivers, and other drivers were GRHL2 and SMURF2. CACNA1E was involved in both TN and luminal BC. The exploratory analysis also indicated a role for SMURF2 in luminal BC development in young patients. Conclusions: The data further indicates that some cancer drivers are more common in a specific breast cancer subtype from young patients, such as TP53 in TNBC and PIK3CA and GATA3 in luminal samples. These results also provide additional evidence that some genes not considered classical cancer-causing genes, such as CACNA1E, GRHL2 and SMURF2 might be cancer drivers in this age group. Objectives triplenegative triple negative Methods Triplenegative Triple sequenced literature Additionally large every 1 years. years . years) Results 2 6 1112 11 12 11/1 91.7 917 91 7 (91. % 1116 16 68.7 687 68 (68. TP NF1 NF NOTCH PTPN13 PTPN PIKCA PIK CA GATA GRHL SMURF CACNAE CACNA E Conclusions TP5 cancercausing causing group 111 11/ 91. 9 (91 68. (68 PTPN1 (9 (6 (

ABSTRACT Soil fertility evaluation is important for adopting conservation management and adequate nutrient supply. The objective of this study was to identify critical levels and soil fertility classes using the boundary line method for rainfed crops (corn, cowpea and sabiá [Mimosa caesalpiniifolia]) in the Brazilian semi-arid region. A database of 226 soil fertility analyses of samples from the 0.00-0.20 m soil layer, and corn, cowpea and sabiá yields from Ceará State was used to generate interpretation classes (at 80 and 95 % of maximum yield). In a scatter plot, soil nutrient concentrations (x-axis) and relative crop yields (y-axis) were correlated, and the border points fitted to a quadratic model. Proposed interpretation classes were classified as very low, low, adequate, high and very high, except for Na+, whose adequate class was considered tolerable. Generated models showed coefficients of determination (R2) for the chemical properties ranging from 0.54 to 0.92. Based on the interpretation classes, the critical level was determined as 6.3 for pH, 10.8 g dm-3 for OM, 20.9 mg dm-3 for P, 81 mg dm-3 for K, 55 mmolc dm-3 for Ca2+, 24 mmolc dm-3 for Mg2+ and 8 mg dm-3 for S-SO42-. Interpretation classes for soils with high-activity clay in the Brazilian semi-arid region were superior to those in the reference literature. Boundary line method established fertility classes and critical levels for soil chemical properties in more than one crop, using the concept of relative yield. supply corn (corn Mimosa caesalpiniifolia caesalpiniifolia] semiarid semi arid 22 0.000.20 000020 0.00 0.20 0 00 20 0.00-0.2 layer at 9 yield . yield) plot xaxis x axis (x-axis yaxis y (y-axis correlated model low Na Na+ tolerable R2 R (R2 054 54 0.5 092 92 0.92 63 6 3 6. pH 108 10 10. dm3 dm dm- OM 209 20. P K 5 Ca2 Ca Ca2+ 2 Mg2 Mg SSO42. SSO42 SSO S SO42 SO S-SO42- highactivity activity literature 000 0.000.2 00002 0.0 020 0.2 0.00-0. (R 05 0. 09 0.9 1 SSO4 SO4 S-SO42 0.000. 0000 02 0.00-0 S-SO4 0.000 0.00- S-SO

ABSTRACT Objectives: To assess the molecular profile of follicular cell-derived thyroid carcinomas (FCDTCs) and correlate the identified mutations with the clinical and pathological features of the affected patients. Materials and methods: Cross-sectional study of tumor samples from 100 adult patients diagnosed with FCDTC between 2010 and 2019. The patients’ clinical and pathological data were collected. Genomic DNA was extracted from formalin-fixed, paraffin-embedded (FFPE) tumors using the ReliaPrep FFPE gDNA Miniprep System. Genotyping of target genomic regions (KRAS, NRAS, BRAF, EGFR, and PIK3CA) was performed using the AmpliSeq panel, while sequencing was performed on the iSeq 100 platform. Results: The patients’ mean age was 39 years. In all, 82% of the tumors were classic papillary thyroid carcinomas. Overall, 54 (54%) tumor samples yielded satisfactory results on next-generation sequencing (NGS), of which 31 harbored mutations. BRAF gene mutations were the most frequent, with the BRAFV600E mutation present in 10 tumors. Seven tumors had BRAFNON-V600E mutations not previously described in FCDTCs (G464E, G464R, G466E, S467L, G469E, G596D, and the T599Ifs*10 deletion) but described in other types of cancer (i.e., skin/melanoma, lung, colorectal, and others). One tumor had a previously reported BRAFA598V mutation. EGFR gene mutations were found in 16 (29%) and KRAS or NRAS alterations in 8 (14%) of the 54 tumors analyzed. Conclusion: We described herein seven non-hotspot/novel variants in the BRAF gene, highlighting their potential role in expanding our understanding of FCDTC genetics. Objectives cellderived cell derived (FCDTCs methods Crosssectional Cross sectional 201 2019 collected formalinfixed, formalinfixed formalin fixed, fixed formalin-fixed paraffinembedded paraffin embedded (FFPE System KRAS, (KRAS PIK3CA PIKCA PIK CA panel platform Results 3 years all 82 Overall 5 54% (54% nextgeneration next generation NGS, NGS , (NGS) frequent BRAFVE BRAFV E 1 BRAFNONV600E BRAFNONVE BRAFNON V600E V G464E, G464E GE G (G464E G464R GR R G466E S467L SL S L G469E G596D GD D T599Ifs10 TIfs T599Ifs T Ifs T599Ifs*1 deletion i.e., ie i e (i.e. skinmelanoma skin melanoma skin/melanoma lung colorectal others. others . others) BRAFAV BRAFA 29% 29 (29% 14% 14 (14% analyzed Conclusion nonhotspot/novel nonhotspotnovel non hotspot/novel hotspot novel genetics 20 (54 (NGS BRAFNONV VE T599Ifs1 T599Ifs* i.e. (i.e 2 (29 (14 nonhotspot hotspotnovel (5 i.e (2 (1 (

ABSTRACT Melon cultivation under saline conditions is a challenge, as the plants can suffer a reduction in fruit production and quality. The aim of this study was to evaluate the production and quality of noble melon fruit fertigated with different saline solutions and K/Ca ratios. The experimental design was of randomised blocks, with treatments arranged in a 3 x 6 factorial scheme, using three melon cultivars (Bazuca, McLaren, County) fertigated with six nutrient solutions [S1 - standard nutrient solution (SNS); S2 - SNS + NaCl (5.0 dS m-1); S3 - S2 enriched with K (50%); S4 - S2 enriched with K (100%); S5 - S2 enriched with Ca (50%); S6 - S2 enriched with Ca (100%)]. At the end of each cultivar cycle, the fruit was harvested and assessed for the following parameters: fresh fruit weight, fruit diameter, internal cavity, pulp thickness, pulp firmness, soluble solids content, titratable acidity, soluble solids/titratable acidity ratio, and vitamin C. Salt stress impaired the physical characteristics of the fruit of the three noble melon cultivars. The nutrient solution enriched with 50% K (S3) did not reduce the effect of salt stress on fresh weight or fruit size in the melon, but afforded an increase in fruit quality. challenge KCa ratios blocks scheme Bazuca, Bazuca (Bazuca McLaren County S1 S [S (SNS) 5.0 50 5 0 (5. m1 m 1 m-1) (50%) 100% 100 (100%) 100%. . (100%)] cycle parameters diameter cavity thickness firmness content solidstitratable ratio C (S3 (SNS 5. (5 m-1 (50% 10 (100% (S ( m- (50 (100 (10 (1

Abstract Background The p53 and p21 proteins are important regulators of cell cycle and apoptosis and may contribute to autoimmune diseases, such as systemic lupus erythematosus (SLE). As genetic polymorphisms may cause changes in protein levels and functions, we investigated associations of TP53 and p21 (CDKN1A) polymorphisms (p53 72 G > C—rs1042522; p53 PIN3—rs17878362; p21 31 C > A—rs1801270; p21 70 C > T—rs1059234) with the development of systemic lupus erythematosus (SLE) in a Southeastern Brazilian population. Methods Genotyping of 353 female volunteers (cases, n = 145; controls, n = 208) was performed by polymerase chain reaction, restriction fragment length polymorphism and/or DNA sequencing. Associations between TP53 and p21 polymorphisms and SLE susceptibility and clinical manifestations of SLE patients were assessed by logistic regression analysis. Results Protective effect was observed for the genotype combinations p53 PIN3 A1/A1 -p21 31 C/A, in the total study population (OR 0.45), and p53 PIN3 A1/A2-p21 31 C/C, in non-white women (OR 0.28). In Whites, p53 72 C-containing (OR 3.06) and p53 PIN3 A2-containing (OR 6.93) genotypes were associated with SLE risk, and higher OR value was observed for the combined genotype p53 72 G/C-p53 PIN3 A1/A2 (OR 9.00). Further, p53 PIN3 A1/A2 genotype was associated with serositis (OR 2.82), while p53 PIN3 A2/A2 and p53 72 C/C genotypes were associated with neurological disorders (OR 4.69 and OR 3.34, respectively). Conclusions Our findings showed that the TP53 and p21 polymorphisms included in this study may have potential to emerge as SLE susceptibility markers for specific groups of patients. Significant interactions of the TP53 polymorphisms with serositis and neurological disorders were also observed in SLE patients. Highlights The polymorphisms TP53 rs1042522 (G > C) and TP53 rs17878362 (16 bp Del/Ins) were associated with SLE risk in whites. In whites, the combined genotype TP53 rs1042522 GC- TP53 rs17878362 A1A2 and the haplotype TP53 rs1042522 C-rs17878362 A2 represented higher SLE risk. Combination of TP53 rs17878362 (16 bp Del/Ins) and p21 rs1801270 (C > A) protected against SLE in non-white women. TP53 and p21 (CDKN1A) polymorphisms may be SLE susceptibility markers for specific groups. p p5 p2 diseases SLE. . functions TP TP5 CDKN1A CDKNA CDKN A (CDKN1A (p5 7 Crs1042522 Crs rs C—rs1042522 PIN3rs17878362 PINrs PIN PIN3—rs17878362 3 Ars1801270 Ars A—rs1801270 Trs1059234 Trs T rs1059234 T—rs1059234 (SLE 35 cases, cases (cases 145 controls 208 reaction andor or sequencing analysis A1A1 AA A1 A1/A -p2 CA C/A 0.45, 045 0.45 , 0 45 0.45) A1/A2p21 A1A2p21 AAp A1/A2-p2 CC nonwhite non white 0.28. 028 0.28 28 0.28) Whites Ccontaining containing 3.06 306 06 A2containing Acontaining 6.93 693 6 93 G/Cp53 GCp53 GCp G/C G/C-p5 9.00. 900 9.00 9 00 9.00) Further 2.82, 282 2.82 2 82 2.82) A2A2 A2/A 469 4 69 4.6 334 34 3.34 respectively. respectively respectively) rs104252 rs1787836 16 (1 Del/Ins DelIns Del Ins whites GC A1A Crs17878362 C-rs1787836 rs180127 (p Crs104252 C—rs104252 PIN3rs1787836 PIN3—rs1787836 Ars180127 A—rs180127 Trs105923 rs105923 T—rs105923 14 20 -p 04 0.4 A2p21 A1/A2p2 A1A2p2 A1/A2-p 02 0.2 3.0 30 6.9 Cp53 Cp G/Cp5 GCp5 G/C-p 90 9.0 2.8 8 A2A 46 4. 33 3.3 rs10425 rs178783 1 ( Crs1787836 C-rs178783 rs18012 Crs10425 C—rs10425 PIN3rs178783 PIN3—rs178783 Ars18012 A—rs18012 Trs10592 rs10592 T—rs10592 0. Ap A2p2 A1/A2p A1A2p 3. 6. Cp5 G/Cp 9. 2. rs1042 rs17878 Crs178783 C-rs17878 rs1801 Crs1042 C—rs1042 PIN3rs17878 PIN3—rs17878 Ars1801 A—rs1801 Trs1059 rs1059 T—rs1059 A2p rs104 rs1787 Crs17878 C-rs1787 rs180 Crs104 C—rs104 PIN3rs1787 PIN3—rs1787 Ars180 A—rs180 Trs105 rs105 T—rs105 rs10 rs178 Crs1787 C-rs178 rs18 Crs10 C—rs10 PIN3rs178 PIN3—rs178 Ars18 A—rs18 Trs10 T—rs10 rs1 rs17 Crs178 C-rs17 Crs1 C—rs1 PIN3rs17 PIN3—rs17 Ars1 A—rs1 Trs1 T—rs1 Crs17 C-rs1 C—rs PIN3rs1 PIN3—rs1 A—rs T—rs C-rs PIN3rs PIN3—rs

Ca3Co4O9 powders and ceramics were synthesized and sintered using microwave-assisted heating method.Another batch of powders was calcined by the conventional heating method and this powder was used to produce ceramics sintered by microwave sintering aiming to compare with those prepared by microwave method. The formation of the phases based on calcination method and time was assessed by X-ray diffractometry. The synthesis and sintering methods affected various properties of the ceramics, including grain size, density, phase amount, Seebeck coefficient, thermal conductivity and electrical conductivity. Microwave heating method reduced the time required for the powder synthesis and sintering of ceramics. Ceramics produced using powder obtained by the conventional method presented Figure of Merit (ZT) values of approximately 0.04. However, these values were reduced for the ceramics produced by microwave heating reaching a value of about 0.02. The evaluation of the thermoelectric properties of the Ca3Co4O9 ceramics suggests that the main issue to enhance the Figure of Merit of the CCO ceramics is related to density and Ca3Co4O9 phase amount present in the ceramics. CaCoO Ca Co O Ca3Co4O microwaveassisted assisted methodAnother Another Xray X ray diffractometry size coefficient ZT (ZT 004 0 04 0.04 However 002 02 0.02 00 0.0 0.

ABSTRACT Apparent calcium (Ca) retention and digestibility coefficients are affected by limestone particle size in the diet of laying hens. This study aimed to determine the apparent retention and digestibility coefficients of Ca in limestone of different particle sizes in laying hens. The study comprised 288 Lohmann Brown laying hens (50 weeks of age; 1,964 ± 98 g) distributed in a completely randomized experimental design with a 3 × 2 factorial arrangement [three Ca concentrations (10, 20, and 30 g kg–1) × two limestone particle sizes (480 and 1,978 µm)] with eight repetitions per treatment and six birds per experimental unit. The experiment included five days for adaptation and five days for total excreta collection. All birds were slaughtered at the end of the ten days to collect the ileal contents. The total or ileal Ca content was plotted against the Ca of diets concentration using linear regression analysis. The regression line slope represented the apparent retention (CaR) and digestibility coefficients of Ca (CaD) in limestone. There was interaction between Ca concentration in the diet and limestone granulometry on CaD (p = 0.001) and CaR (p < 0.001). The CaD and CaR of fine- and coarse-grained limestone increased linearly with increasing Ca concentrations in the diet. The apparent digestibility coefficients estimated for laying hens fed with fine-grained and coarse-grained limestone were 0.72 and 0.35, respectively. The apparent retention coefficients estimated for laying hens fed fine-grained and coarse-grained limestone were 0.96 and 0.47, respectively.

Resumo Antecedentes A telemedicina permite que pacientes com doença de Parkinson (DP) superem barreiras físicas para acessar serviços de saúde e aumenta a acessibilidade para pessoas com mobilidade reduzida. Objetivo Investigar indicadores de viabilidade de uma intervenção em telessaúde para pacientes com DP, incluindo recrutamento, atendimento, aderência, problemas técnicos, satisfação e benefícios nos níveis de atividade física e sono. Métodos Foi conduzido um estudo de centro e braço únicos baseado em consultas por telessaúde com utilização do WhatsApp (Meta Platforms, Inc., Menlo Park, CA, EUA). Foram calculados indicadores de viabilidade como desfechos primários. Resultados As taxas de recrutamento, atendimento e problemas técnicos foram 61,3%, 90,5% e 13,3%, respectivamente, com bons escores de aceitação e satisfação com a intervenção. A intervenção melhorou os níveis de atividade física, incluindo o número de passos por pelo menos 10 minutos contínuos (p = 0,009) e o número de atividades intensas e moderadas com duração de pelo menos 10 minutos contínuos (p = 0,001). O Índice de Qualidade do Sono de Pittsburgh melhorou nos seguintes componentes: duração percebida do sono (p < 0,001) e escore total (p < 0,001). A média do tempo de viagem médio poupado foi de 289,6 minutos, e a economia financeira foi de R$ 106,67 reais (por volta de USD 18; quase 10% do salário mínimo atual do Brasil). Conclusões As consultas por vídeo provaram ser viáveis e efetivas, com impacto positivo nos níveis de atividade física e sono de pacientes com DP. DP (DP reduzida recrutamento aderência Meta Platforms Inc Inc. Park CA EUA. EUA . EUA) primários 613 61 3 61,3% 905 90 5 90,5 133 13 13,3% respectivamente 1 p 0,009 0009 0 009 0,001. 0001 0,001 001 componentes 2896 289 6 289, R 10667 106 67 106,6 18 Brasil. Brasil Brasil) efetivas 61,3 9 90, 13,3 0,00 000 00 28 1066 106, 61, 13, 0,0 2 0,

Abstract Background Telemedicine allows Parkinson disease (PD) patients to overcome physical barriers to access health care services and increases accessibility for people with mobility impairments. Objective To investigate the feasibility indicators of a telehealth intervention for PD patients, including patient recruitment, attendance, technical issues, satisfaction, and benefits on levels of physical activity and sleep. Methods We conducted a single-center, single-arm study of telehealth video consultations using WhatsApp (Meta Platforms, Inc., Menlo Park, CA, USA). Also, we collected the feasibility indicators as the primary endpoints. All the patients in the study were previously evaluated in person by the same team. Results Patient recruitment, attendance, and technical issues rates were 61.3%, 90.5%, and 13.3%, respectively, with good scores of patient acceptance and satisfaction with the study intervention. The telehealth intervention improved physical activity, including the number of walks for at least 10 continuous minutes (p = 0.009) and the number of moderate-intensity activities lasting at least 10 continuous minutes (p = 0.001). The Pittsburgh sleep quality index (PSQI) scores also improved for one of its components: perceived sleep duration (p < 0.001) and for total Pittsburgh score (p < 0,001). The average travel time saving was 289.6 minutes, and money-saving was R$106.67 (around USD 18; almost 10% of the current minimum wage in Brazil). Conclusions Direct-to-patient telehealth video consultations proved to be feasible and effective and had a positive impact on physical activity levels and sleep in PD patients. (PD impairments recruitment attendance singlecenter, singlecenter single center, center single-center singlearm arm Meta Platforms Inc Inc. Park CA USA. USA . USA) Also endpoints team 613 61 3 61.3% 905 90 5 90.5% 133 13 13.3% respectively 1 p 0.009 0009 0 009 moderateintensity moderate intensity 0.001. 0001 0.001 001 PSQI (PSQI components 0,001. 0,001 0,001) 2896 289 6 289. moneysaving money R10667 R 106 67 R$106.6 around 18 Brazil. Brazil Brazil) Directtopatient Direct 61.3 9 90.5 13.3 0.00 000 00 0,00 28 R1066 R$106. 61. 90. 13. 0.0 0,0 2 R106 R$106 0. 0, R10 R$10 R1 R$1 R$

ABSTRACT. In Brazil, although there is a growing demand for physalis in the food industries, information on the nutritional management of the crop is still incipient. This study aimed to evaluate the biomass production and accumulation of nutrients in the shoots and fruit of physalis in two cultivation sites. Two field experiments were conducted, one where the soil was Typic Quartzipsamment in the municipality of Diamantina and the other where the soil was Rhodic Hapludox in the municipality of São João Evangelista, Minas Gerais State, Brazil. The experiments were conducted in a randomized complete block design with four replicates. The treatments were the evaluation period. Samples were collected every 15 days the day of planting of the seedlings in the field for a period of 240 days. Dry matter and nutrient accumulation in shoot and fruit were evaluated. The biomass production and nutrient accumulation were higher in the edaphoclimatic conditions at São João Evangelista compared to the conditions at Diamantina. Macronutrient accumulation was in the following order: N > K > Ca > S > Mg > P in shoot and N > K > P > S > Ca > Mg in fruit. Micronutrient accumulation in shoot and fruit was in the following order: Fe > Mn > Zn > B > Cu.

RESUMO A pitaia é uma cultura carente em informações a respeito do manejo nutricional. O conhecimento sobre o crescimento e o acúmulo de nutrientes nas diferentes fases de desenvolvimento da planta auxiliará na elaboração de programas de adubação racionais para a cultura, diminuindo os custos de produção. Objetivou-se com este trabalho avaliar o crescimento e o acúmulo de nutrientes na pitaia saborosa ao longo do tempo de cultivo. Cladódios de pitaia da espécie Hylocereus setaceus foram cultivados em vasos de polietileno, contendo um Argissolo Vermelho-Amarelo Eutrófico e conduzidas a céu aberto. Os tratamentos constituíram-se de seis épocas de amostragens: 0, 60, 120, 180, 240, 300 e 360 dias após o plantio e o delineamento experimental utilizado foi em blocos casualizados, com quatro repetições. Em cada época foram amostradas quatro plantas para avaliar o crescimento e o acúmulo de nutrientes. A pitaia saborosa apresentou crescimento exponencial até 360 dias após o plantio. Observou-se alta absorção de nutrientes no período de 300 a 360 dias após o plantio. O acúmulo de nutrientes nos cladódios, em ordem decrescente, foi: 3.91 (K), 2.56 (Ca), 1.95 (N), 1.24 (P), 0.45 (Mg), 0.30 (S) e 0.06 (Na) g planta-1, e 14.86 (Zn), 12.72 (Fe), 12.37 (Mn), 5.37 (B) e1.04 (Cu) mg plant-1. A TCR e a TARN apresentaram maiores valores entre 60 e 120 dias. nutricional produção Objetivouse Objetivou se cultivo polietileno VermelhoAmarelo Vermelho Amarelo aberto constituíramse constituíram amostragens 0 180 240 30 36 casualizados repetições Observouse Observou cladódios decrescente 391 3 91 3.9 K, K , (K) 256 2 56 2.5 Ca, Ca (Ca) 195 1 95 1.9 N, N (N) 124 24 1.2 P, P (P) 045 45 0.4 Mg, Mg (Mg) 030 0.3 S (S 006 06 0.0 Na (Na planta1, planta1 1, planta-1 1486 14 86 14.8 Zn, Zn (Zn) 1272 12 72 12.7 Fe, Fe (Fe) 1237 37 12.3 Mn, Mn (Mn) 537 5 5.3 B (B e104 e1 04 e1.0 Cu (Cu plant1. plant1 plant 1. plant-1 6 18 39 9 3. (K 25 2. (Ca 19 (N (P 4 0. (Mg 03 00 planta- 148 8 14. (Zn 127 7 12. (Fe 123 (Mn 53 5. e10 e1. plant-

ABSTRACT Information on nutritional management of pitaya crops are scarce. However, understanding the growth and nutrient accumulation in these plants at different developmental stages can assist in the development of rational soil fertilizer application programs for pitaya crops and decrease production costs. Thus, the objective of this work was to evaluate the growth and nutrient accumulation in pitaya plants throughout the crop cycle. Cladodes of pitaya plants of the Hylocereus setaceus species were grown in polyethylene pots containing a Typic Hapludult (Argissolo Vermelho-Amarelo Eutrófico) under full sun. The treatments consisted of six sampling times: 0, 60, 120, 180, 240, 300, and 360 days after planting (DAP). A randomized block experimental design with four replications was used. Four plants were sampled and evaluated for growth and nutrient accumulation at each sampling time. The pitaya plants presented an exponential growth up to 360 DAP and high nutrient absorption between 300 and 360 DAP. The nutrient and Na accumulations in the cladodes, in decreasing order, were: 3.91 (K), 2.56 (Ca), 1.95 (N), 1.24 (P), 0.45 (Mg), 0.30 (S), and 0.06 (Na) g plant-1, and 14.86 (Zn), 12.72 (Fe), 12.37 (Mn), 5.37 (B), and 1.04 (Cu) mg plant-1. The highest relative growth rate and relative nutrient absorption rate were found between 60 and 120 DAP. scarce However costs Thus cycle Argissolo VermelhoAmarelo Vermelho Amarelo Eutrófico sun times 0 180 240 36 . (DAP) used time 30 cladodes order 391 3 91 3.9 K, K , (K) 256 2 56 2.5 Ca, Ca (Ca) 195 1 95 1.9 N, N (N) 124 24 1.2 P, P (P) 045 45 0.4 Mg, Mg (Mg) 030 0.3 S, S (S) 006 06 0.0 (Na plant1, plant1 plant 1, plant-1 1486 14 86 14.8 Zn, Zn (Zn) 1272 12 72 12.7 Fe, Fe (Fe) 1237 37 12.3 Mn, Mn (Mn) 537 5 5.3 B, B (B) 104 04 1.0 Cu (Cu plant1. 1. 6 18 (DAP 39 9 3. (K 25 2. (Ca 19 (N (P 4 0. (Mg 03 (S 00 plant- 148 8 14. (Zn 127 7 12. (Fe 123 (Mn 53 5. (B 10

Resumo O presente estudo investigou a relação entre cinco polimorfismos genéticos e o desenvolvimento de mucosite oral em pacientes pediátricos recebendo quimioterapia com metrotexato. O estudo longitudinal foi conduzido com 64 pacientes e a mucosite oral avaliada pelo Oral Assessment Guide modificado, que tem como objetivo diagnosticar e classificar a mucosite oral. Células epiteliais bucais foram obtidas por bochecho e o DNA foi extraído. Os polimorfismos MTHFR (rs1801133), DNMT3B (rs2424913), ABCC2 (rs717620), ABCG2 (rs2231137) e ABCG2 (rs2231142), foram analisados pela técnica de PCR-RFLP. Dados demográficos, hematológicos e bioquímicos foram coletados a partir de registros médicos. Análise estatística foi realizada utilizando o software SPSS adotando um valor de p=0,05. Observou-se que, o sexo masculino foi predominante (56,2%), e a idade média foi de 10,8 anos (± 4.9). A mucosite oral acometeu 65,6% dos pacientes, dos quais, 61,9% desenvolveram a forma grave da doença. Para o gene ABCG2 (rs2231142), o alelo raro A e o genótipo CA foram mais frequentes em indivíduos com mucosite (p= 0.02; RR = 0.60; CI = 0.387 - 0.813). A gravidade da doença foi observada principalmente em pacientes mais jovens (mediana = 9 anos; p=0.02). Além disso, os pacientes com mucosite oral grave apresentaram menor contagem de leucócitos (mediana = 2150 mm3) em comparação aos pacientes com a forma leve/moderada (mediana = 4200 mm3; p=0.03). Pacientes do sexo feminino e aumento a cada 10.000 plaquetas foram fatores de proteção contra o aparecimento de mucosite oral (p=0.02). Concluiu-se que a presença do polimorfismo rs2231142 aumenta o risco de o paciente desenvolver a mucosite oral, bem como pacientes mais jovens e menor contagem de leucócitos contribui com a severidade.

Abstract The study investigated the relationship between genetic polymorphisms and the development of oral mucositis in pediatric patients undergoing chemotherapy involving methotrexate. A longitudinal study was conducted with 64 patients, and oral mucositis was evaluated by the modified Oral Assessment Guide, which aims to diagnose and classify oral mucositis. Epithelial cells were obtained by mouthwash and DNA was extracted. The polymorphisms MTHFR (rs1801133), DNMT3B (rs2424913), ABCC2 (rs717620), ABCG2 (rs2231137) and ABCG2 (rs2231142) were analyzed by PCR-RFLP method. Demographic, hematological and biochemical data were collected from medical records. Statistical analysis was performed using the SPSS software adopting a p-value of 0.05. Male sex predominated (56.2%), and the mean age was 10.8 years (± 4.9). Oral mucositis affected 65.6% of the patients, of which 61.9% developed the severe form of the disease. For the ABCG2 gene (rs2231142), the rare A allele and CA genotype were more frequent in individuals with mucositis (p= 0.02; RR = 0.60; CI = 0.387 - 0.813). The severity of the disease was mainly observed in younger patients (median = 9 years; p=0.02). Patients with severe oral mucositis presented lower leukocytes count (median = 2.150 mm3) compared to patients with the mild/moderate form (median = 4.200 mm3; p=0.03). Female patients and each 10,000-platelet increase were protective factors against the onset of oral mucositis (p=0.02). It is concluded that rs2231142 polymorphism increases the likelihood of oral mucositis and younger patients and patients with low leukocytes counts are more likely to develop severe form.