The corrosion behavior of Al0.8CrFeCoNiCu0.5Six (x=0, 0.2, 0.3) high-entropy alloy laser cladding coatings in a 3.5% NaCl solution was investigated using polarization tests, impedance spectroscopy, scanning electron microscopy, and energy dispersive spectroscopy. As the Si content increased, the over-passivation potential and the width of the passivation zone for the Al0.8CrFeCoNiCu0.5Six coating expanded, while the corrosion current density tended to decrease, enhancing the stability of the passivation film and the corrosion resistance of the coating. The Al0.8CrFeCoNiCu0.5 coating underwent selective corrosion, with some pitting corrosion distribution showing no apparent regularity. The Al0.8CrFeCoNiCu0.5Si0.2 coating exhibited a distinct grain profile with a tendency towards intergranular corrosion. The aggregation of Cu-rich and Al-Ni intergranular phases in the eutectic structure led to preferential erosion at the grain boundaries by corrosion ions in the Al0.8CrFeCoNiCu0.5Si0.2 coating. The Al0.8CrFeCoNiCu0.5Si0.3 coating presented corrosion pits, although their distribution did not display apparent regularity. The Al0.8CrFeCoNiCu0.5Six high-entropy alloy coating demonstrates superior corrosion resistance in solution compared to 5083 aluminum alloy. Al08CrFeCoNiCu05Six AlCrFeCoNiCuSix Al0 8CrFeCoNiCu0 5Six Al CrFeCoNiCu Six x=0, x0 x 0 (x=0 02 2 0.2 0.3 03 3 highentropy high entropy 35 5 3.5 tests spectroscopy microscopy increased overpassivation over expanded decrease Al08CrFeCoNiCu05 AlCrFeCoNiCu Al0.8CrFeCoNiCu0. regularity Al08CrFeCoNiCu05Si02 AlCrFeCoNiCuSi 5Si0 Al0.8CrFeCoNiCu0.5Si0. Curich Cu rich AlNi Ni Al08CrFeCoNiCu05Si03 pits 508 8CrFeCoNiCu x=0 (x= 0. 3. Al08CrFeCoNiCu0 Al0.8CrFeCoNiCu0 Al08CrFeCoNiCu05Si0 5Si Al0.8CrFeCoNiCu0.5Si0 50 x= (x Al08CrFeCoNiCu Al0.8CrFeCoNiCu Al08CrFeCoNiCu05Si Al0.8CrFeCoNiCu0.5Si

ABSTRACT Objective: The purpose of present study was to comprehensively explore the efficacy and safety of prothrombin complex concentrate (PCC) to treat massive bleeding in patients undergoing cardiac surgery. Methods: PubMed®, Embase, and Cochrane Library databases were searched for studies investigating PCC administration during cardiac surgery published before September 10, 2022. Mean difference (MD) with 95% confidence interval (CI) was applied to analyze continuous data, and dichotomous data were analyzed as risk ratio (RR) with 95% CI. Results: Twelve studies were included in the meta-analysis. Compared with other non-PCC treatment regimens, PCC was not associated with elevated mortality (RR=1.18, 95% CI=0.86–1.60, P=0.30, I2=0%), shorter hospital stay (MD=-2.17 days; 95% CI=-5.62–1.28, P=0.22, I2=91%), reduced total thoracic drainage (MD=-67.94 ml, 95% CI=-239.52–103.65, P=0.44, I2=91%), thromboembolic events (RR=1.10, 95% CI=0.74–1.65, P=0.63, I2=39%), increase in atrial fibrillation events (RR=0.73, 95% CI=0.52–1.05, P=0.24, I2=29%), and myocardial infarction (RR=1.10, 95% CI=0.80–1.51, P=0.57, I2=81%). However, PCC use was associated with reduced intensive care unit length of stay (MD=-0.81 days, 95% CI=-1.48– -0.13, P=0.02, I2=0%), bleeding (MD=-248.67 ml, 95% CI=-465.36– -31.97, P=0.02, I2=84%), and intra-aortic balloon pump/extracorporeal membrane oxygenation (RR=0.65, 95% CI=0.42–0.996, P=0.05, I2=0%) when compared with non-PCC treatment regimens. Conclusion: The use of PCC in cardiac surgery did not correlate with mortality, length of hospital stay, thoracic drainage, atrial fibrillation, myocardial infarction, and thromboembolic events. However, PCC significantly improved postoperative intensive care unit length of stay, bleeding, and intra-aortic balloon pump/ extracorporeal membrane oxygenation outcomes in patients undergoing cardiac surgery. Objective (PCC Methods PubMed PubMed® Embase 10 2022 MD (MD 95 CI (CI RR (RR Results metaanalysis. metaanalysis meta analysis. analysis meta-analysis nonPCC non regimens RR=1.18, RR118 1 18 (RR=1.18 CI086160 0 86 60 CI=0.86–1.60 P030 P 30 P=0.30 I2=0%, I20 I I2=0% , I2 MD=2.17 MD217 MD= 2.17 2 17 (MD=-2.1 days CI=5.62–1.28, CI562128 CI= 5.62–1.28, 5 62 28 CI=-5.62–1.28 P022 22 P=0.22 I2=91%, I291 I2=91% 91 I2=91%) MD=67.94 MD6794 67.94 67 94 (MD=-67.9 ml CI=239.52–103.65, CI2395210365 239.52–103.65, 239 52 103 65 CI=-239.52–103.65 P044 44 P=0.44 RR=1.10, RR110 (RR=1.10 CI074165 74 CI=0.74–1.65 P063 63 P=0.63 I2=39%, I239 I2=39% 39 I2=39%) RR=0.73, RR073 73 (RR=0.73 CI052105 05 CI=0.52–1.05 P024 24 P=0.24 I2=29%, I229 I2=29% 29 I2=29%) CI080151 80 51 CI=0.80–1.51 P057 57 P=0.57 I2=81%. I281 I2=81% . 81 I2=81%) However MD=0.81 MD081 0.81 (MD=-0.8 CI=1.48– CI148 1.48– 48 CI=-1.48 0.13, 013 13 -0.13 P002 02 P=0.02 MD=248.67 MD24867 248.67 248 (MD=-248.6 CI=465.36– CI46536 465.36– 465 36 CI=-465.36 31.97, 3197 31 97 -31.97 I2=84%, I284 I2=84% 84 I2=84%) intraaortic intra aortic pumpextracorporeal pump RR=0.65, RR065 (RR=0.65 CI0420996 42 996 CI=0.42–0.996 P005 P=0.05 Conclusion 202 9 RR=1.18 RR11 (RR=1.1 CI08616 8 6 CI=0.86–1.6 P03 3 P=0.3 I2=0 MD=2.1 MD21 217 2.1 (MD=-2. CI=5.62–1.28 CI56212 562128 5.62–1.28 CI=-5.62–1.2 P02 P=0.2 I29 I2=91 MD=67.9 MD679 6794 67.9 (MD=-67. CI=239.52–103.65 CI239521036 2395210365 239.52–103.65 23 CI=-239.52–103.6 P04 4 P=0.4 RR=1.10 CI07416 7 CI=0.74–1.6 P06 P=0.6 I23 I2=39 RR=0.73 RR07 (RR=0.7 CI05210 CI=0.52–1.0 I22 I2=29 CI08015 CI=0.80–1.5 P05 P=0.5 I28 I2=81 MD=0.8 MD08 081 0.8 (MD=-0. CI=1.48 CI14 148 1.48 CI=-1.4 0.13 01 -0.1 P00 P=0.0 MD=248.6 MD2486 24867 248.6 (MD=-248. CI=465.36 CI4653 46536 465.36 46 CI=-465.3 31.97 319 -31.9 I2=84 RR=0.65 RR06 (RR=0.6 CI042099 99 CI=0.42–0.99 20 RR=1.1 RR1 (RR=1. CI0861 CI=0.86–1. P0 P=0. I2= MD=2. MD2 21 2. (MD=-2 CI=5.62–1.2 CI5621 56212 5.62–1.2 CI=-5.62–1. I2=9 MD=67. MD67 679 67. (MD=-67 CI=239.52–103.6 CI23952103 239521036 239.52–103.6 CI=-239.52–103. CI0741 CI=0.74–1. I2=3 RR=0.7 RR0 (RR=0. CI0521 CI=0.52–1. I2=2 CI0801 CI=0.80–1. I2=8 MD=0. MD0 08 0. (MD=-0 CI=1.4 CI1 14 1.4 CI=-1. 0.1 -0. MD=248. MD248 2486 248. (MD=-248 CI=465.3 CI465 4653 465.3 CI=-465. 31.9 -31. RR=0.6 CI04209 CI=0.42–0.9 RR=1. (RR=1 CI086 CI=0.86–1 P=0 MD=2 (MD=- CI=5.62–1. CI562 5621 5.62–1. CI=-5.62–1 MD=67 MD6 (MD=-6 CI=239.52–103. CI2395210 23952103 239.52–103. CI=-239.52–103 CI074 CI=0.74–1 RR=0. (RR=0 CI052 CI=0.52–1 CI080 CI=0.80–1 MD=0 CI=1. 1. CI=-1 -0 MD=248 MD24 (MD=-24 CI=465. CI46 465. CI=-465 31. -31 CI0420 CI=0.42–0. RR=1 (RR= CI08 CI=0.86– P= (MD= CI=5.62–1 CI56 562 5.62–1 CI=-5.62– MD=6 CI=239.52–103 CI239521 2395210 239.52–103 CI=-239.52–10 CI07 CI=0.74– RR=0 CI05 CI=0.52– CI=0.80– CI=1 CI=- - MD=24 CI=465 CI4 CI=-46 -3 CI042 CI=0.42–0 RR= CI0 CI=0.86 CI=5.62– CI5 56 5.62– CI=-5.62 CI=239.52–10 CI23952 239521 239.52–10 CI=-239.52–1 CI=0.74 CI=0.52 CI=0.80 CI=46 CI=-4 CI04 CI=0.42– CI=0.8 CI=5.62 5.62 CI=-5.6 CI=239.52–1 CI2395 23952 239.52–1 CI=-239.52– CI=0.7 CI=0.5 CI=4 CI=0.42 CI=0. CI=5.6 5.6 CI=-5. CI=239.52– CI239 2395 239.52– CI=-239.52 CI=0.4 CI=0 CI=5. 5. CI=-5 CI=239.52 CI23 239.52 CI=-239.5 CI=5 CI=239.5 CI2 239.5 CI=-239. CI=239. 239. CI=-239 CI=239 CI=-23 CI=23 CI=-2 CI=2

ABSTRACT This study was conducted to identify the key volatile compounds that characterize whether silage has deteriorated and to investigate the intrinsic link between the key compounds and silage odor. First, silages with different aerobic exposure durations were sampled, and sensory evaluation integrating aerobic stability monitoring was used to distinguish whether the silage had deteriorated. Subsequently, headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) was utilized to determine the relative content of the compounds. Using the relative content of the compounds in each silage as input, relative odor activity value (ROAV) calculations and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed to determine the odor contribution of the compounds and the compounds with significant differences in relative content (based on variable importance for the projection, VIP) between the deteriorated and non-deteriorated silages. Next, the key compounds were identified by combining the conditions of average ROAV (aROAV) ≥ 1 and VIP > 1. Finally, the OPLS algorithm was used to analyze the intrinsic link of key compounds with the silage odor. The results showed that three out of 63 compounds—4-ethyl phenol, eugenol, and ethyl linoleate—were key compounds to characterize whether the silages deteriorated or not. In addition, ortho-guaiacol, 4-ethyl guaiacol, and 2-methoxy-4-vinyl phenol were the specific key compounds for deteriorated silage. Eugenol and ethyl linoleate were correlated with fruity, sour, and spicy odors. In addition, guaiacol, 4-ethylphenol, 4-ethyl guaiacol and 2-methoxy-4-vinyl phenol contributed to roasted, musty, and putrid odors. First sampled Subsequently solidphase solid phase chromatographymass chromatography mass HSSPMEGCMS HS SPME GC MS (HS-SPME-GC-MS input (ROAV OPLSDA DA (OPLS-DA based projection nondeteriorated non Next aROAV (aROAV Finally 6 compounds4ethyl compoundsethyl 4 eugenol linoleatewere not addition orthoguaiacol, orthoguaiacol ortho ortho-guaiacol 4ethyl 2methoxy4vinyl methoxyvinyl 2 methoxy vinyl fruity sour odors 4ethylphenol, 4ethylphenol ethylphenol ethylphenol, 4-ethylphenol roasted musty

Abstract Background The underlying mechanism of chronic pain involves the plasticity in synaptic receptors and neurotransmitters. This study aimed to investigate potential roles of Neuroligins (NLs) within the spinal dorsal horn of rats in a newly established Bone Cancer Pain (BCP) model. The objective was to explore the mechanism of neuroligin involved in the occurrence and development of bone cancer pain. Methods Using our rat BCP model, we assessed pain hypersensitivity over time. Quantitative real-time polymerase chain reaction and Western blot analysis were performed to investigate NL expression, and NLs were overexpressed in the rat spinal cord using lentiviral vectors. Immunofluorescence staining and whole-cell patch-clamp recordings were deployed to investigate the role of NLs in the development of BCP. Results We observed reduced expression levels of NL1 and NL2, but not of NL3, within the rat spinal cord, which were found to be associated with and essential for the development of BCP in our model. Accordingly, NL1 or NL2 overexpression in the spinal cord alleviated mechanical hypersensitivity of rats. Electrophysiological experiments indicated that NL1 and NL2 are involved in BCP via regulating γ-aminobutyric acid-ergic interneuronal synapses and the activity of glutamatergic interneuronal synapses, respectively. Conclusions Our observations unravel the role of NLs in cancer-related chronic pain and further suggest that inhibitory mechanisms are central features of BCP in the spinal dorsal horn. These results provide a new perspective and basis for subsequent studies elucidating the onset and progression of BCP. neurotransmitters (NLs (BCP model time realtime real vectors wholecell whole cell patchclamp patch clamp NL3 Accordingly γaminobutyric γ aminobutyric acidergic acid ergic respectively cancerrelated related

This study aimed to analyze the safety and applicability of a 90-min duration of infusion (SDI) of obinutuzumab in patients with B-cell non-Hodgkin's lymphoma (NHL) in a tertiary hospital in China. This exploratory clinical trial was performed at Jiangsu Province Hospital. All patients were treated with the standard infusion regimen for the first infusion. If no grade ≥3 infusion-related reactions (IRRs) occurred, the subsequent infusions were given as SDI. The primary endpoint was the incidence of IRR during the standard infusion (3-4 h) and 90-min SDI regimens. This study enrolled 208 patients and all completed cycle 1. Forty-one patients (19.71%) had IRRs: five (2.40%) with grade 1, twenty-eight (13.46%) with grade 2, and eight (3.85%) with grade 3. The 41 patients had 71 IRRs, mainly fever (40.85%), chest pain/tightness (12.68%), and dyspnea (9.86%). The occurrence of IRRs in the first infusion was significantly lower in patients who received oral acetaminophen prophylaxis than those who did not (10.72% vs 30.21%, P<0.001). For the subsequent cycles with 90-min SDI, only two (0.25%) IRRs occurred among 814 infusions (one grade 1 hand numbness and one grade 2 chill/fever). The 90-min obinutuzumab SDI might be safe and feasible in patients with B-cell NHL in China. 90min min 90 (SDI Bcell B cell nonHodgkins non Hodgkin s (NHL China Hospital 3 ≥ infusionrelated related (IRRs 34 4 (3- h regimens 20 Fortyone Forty 19.71% 1971 19 (19.71% 2.40% 240 40 (2.40% twentyeight twenty 13.46% 1346 13 46 (13.46% 3.85% 385 85 (3.85% 7 40.85%, 4085 40.85% , (40.85%) paintightness pain tightness 12.68%, 1268 12.68% 12 68 (12.68%) 9.86%. 986 9.86% . 9 86 (9.86%) 10.72% 1072 10 72 (10.72 3021 30 21 30.21% P<0.001. P0001 P P<0.001 0 001 P<0.001) 0.25% 025 25 (0.25% 81 chill/fever. chillfever chill/fever chill chill/fever) (3 19.71 197 (19.71 2.40 24 (2.40 13.46 134 (13.46 3.85 38 8 (3.85 408 40.85 (40.85% 126 12.68 6 (12.68% 98 9.86 (9.86% 10.72 107 (10.7 302 30.21 P000 P<0.00 00 0.25 02 (0.25 ( 19.7 (19.7 2.4 (2.4 13.4 (13.4 3.8 (3.8 40.8 (40.85 12.6 (12.68 9.8 (9.86 10.7 (10. 30.2 P00 P<0.0 0.2 (0.2 19. (19. 2. (2. 13. (13. (3. 40. (40.8 12. (12.6 9. (9.8 10. (10 30. P0 P<0. 0. (0. (19 (2 (13 (40. (12. (9. (1 P<0 (0 (40 (12 (9 P< (4

RESUMEN Objetivo: Explorar el efecto de la estimulación eléctrica y la terapia de biorretroalimentación en pacientes con prolapso posparto de órganos pélvicos e identificar los factores que pueden afectar los resultados de la eficacia terapéutica. Método: Este estudio es un análisis retrospectivo de los datos clínicos de pacientes con prolapso posparto de órganos pélvicos. Entre marzo de 2019 y marzo de 2022, se reclutaron un total de 328 mujeres con prolapso de órganos pélvicos en las seis semanas posteriores al parto en un hospital terciario de la provincia de Sichuan, China. Los factores pronósticos de eficacia terapéutica se analizaron mediante regresión logística y el modelo de árbol de decisión. Resultados: En total, 259 mujeres mostraron beneficios clínicos relacionados con el tratamiento. El modelo de regresión logística mostró que la paridad, el entrenamiento en casa de la musculatura del suelo pélvico y la etapa de cuantificación del prolapso de órganos pélvicos antes del tratamiento fueron factores pronósticos independientes. El modelo de árbol de decisión mostró que la etapa de cuantificación del prolapso de órganos pélvicos previa al tratamiento fue el principal factor pronóstico, seguido de la paridad. No hubo diferencias significativas en el área bajo la curva ROC entre los dos modelos. Conclusión: La paridad, el entrenamiento en casa de la musculatura del suelo pélvico y la etapa de cuantificación del prolapso de órganos pélvicos previa al tratamiento fueron factores pronósticos importantes de la estimulación eléctrica y la terapia de biorretroalimentación en el prolapso posparto de órganos pélvicos. Objetivo Método 201 2022 32 Sichuan China Resultados 25 paridad independientes pronóstico modelos Conclusión 20 202 3 2

RESUMO Objetivo: Explorar o efeito da estimulação elétrica e da terapia de biofeedback em pacientes com prolapso de órgãos pélvicos pós-parto e identificar fatores que podem afetar os resultados da eficácia terapêutica. Método: Este estudo é uma análise retrospectiva de dados clínicos de pacientes com prolapso de órgãos pélvicos pós-parto. Um total de 328 mulheres com prolapso de órgãos pélvicos nas seis semanas pós-parto foram recrutadas em um hospital terciário na província de Sichuan, na China, entre março de 2019 e março de 2022. Os fatores prognósticos de eficácia terapêutica foram analisados por meio de regressão logística e modelo de árvore de decisão. Resultados: No geral, 259 mulheres apresentaram benefícios clínicos com o tratamento. O modelo de regressão logística mostrou que a paridade, o treinamento muscular do assoalho pélvico em casa e o estágio de quantificação do prolapso de órgãos pélvicos antes do tratamento foram fatores prognósticos independentes. O modelo de árvore de decisão mostrou que o estágio de quantificação do prolapso de órgãos pélvicos antes do tratamento foi o principal fator prognóstico, seguido pela paridade. Não houve diferença significativa na área sob a curva ROC entre os dois modelos. Conclusão: A paridade, o treinamento muscular do assoalho pélvico em casa e o estágio de quantificação do prolapso de órgãos pélvicos antes do tratamento foram importantes fatores prognósticos da estimulação elétrica e da terapia de biofeedback no prolapso de órgãos pélvicos pós-parto. Objetivo pósparto pós parto Método pósparto. parto. 32 Sichuan China 201 2022 Resultados geral 25 paridade independentes prognóstico modelos Conclusão 3 20 202 2

ABSTRACT Objective: To explore the effects of electric stimulation and biofeedback therapy in patients with postpartum pelvic organ prolapse and to identify factors that can affect therapeutic efficacy outcomes. Method: This retrospective study analysed clinical data about patients with postpartum pelvic organ prolapse. A total of 328 women with pelvic organ prolapse at 6 weeks postpartum were recruited from one tertiary hospitals in Sichuan province in China, between March 2019 and March 2022. The prognostic factors of therapeutic efficacy were analysed using logistic regression and decision tree model. Results: Overall, 259 women showed clinical benefits from the treatment. The logistic regression model showed that parity, pelvic floor muscle training at home, and the pelvic organ prolapse quantitation stage before treatment were independent prognostic factors. The decision tree model showed that the pelvic organ prolapse quantitation stage before treatment was the main prognostic factor, followed by parity. There was no significant difference in the area under the receiver operating characteristic curve between the two models. Conclusion: Parity, pelvic floor muscle training at home, and the pelvic organ prolapse quantitation stage before treatment were important prognostic factors of electric stimulation and biofeedback therapy on postpartum pelvic organ prolapse. Objective outcomes Method 32 China 201 2022 Results Overall 25 parity home factor models Conclusion Parity 3 20 202 2

Abstract Introduction: Propofol is a widely used anesthetic and its dose is closely related to aging. Telomere length (TL) is a unique heritable trait, and emerging as a biomarker of aging, health and disease. Telomerase RNA component (TERC) plays an important role in maintaining TL. We proposed a hypothesis that propofol dose in general anesthesia can be predicted by measuring TL before operation, which greatly reduced the risk of anesthesia, especially the elderly. Methods: The association between the propofol dose in anesthesia induction and: TL in the DNA of peripheral blood leukocytes; body weight; sex; difference of the Bispectral Index (BIS) before and after anesthesia induction in patients was evaluated by multivariable linear regression analyses. The mutation at the 5’end or 3’end of TERC was detected. We recruited 100 patients of elective surgery. Results: We found that propofol dose in anesthesia induction was clearly correlated significantly with TL (r = 0.78, p < 0.001), body weight (r = 0.84, p = 0.004), sex (r = 0.83, p= 0.84, p = 0.004), sex (r = 0.83, p = 0.004), and difference of BIS before and after anesthesia induction (r = 0.85, p = 0.029). By comparing the absolute values of standardized regression coefficients (0.58, 0.21, 0.19, and 0.12) of the four variables, it can be seen that TL contributes the most to the propofol dose in anesthesia induction. However, the mutation at the 5’ end or 3’ end of TERC was not found. Conclusions: These findings provide preliminary evidence that the propofol dose in anesthesia induction was clearly correlated with genetically determined TL. TL may be a promising predictor of the propofol dose, which is beneficial to improve the safety of anesthesia and reduce perioperative complications. Introduction aging (TL trait disease (TERC operation elderly Methods leukocytes (BIS analyses 5end 5 3end 3 detected 10 surgery Results r 078 0 78 0.78 0.001, 0001 0.001 , 001 0.001) 084 84 0.84 0.004, 0004 0.004 004 0.004) 083 83 0.83 085 85 0.85 0.029. 0029 0.029 . 029 0.029) 0.58, 058 58 (0.58 021 21 0.21 019 19 0.19 0.12 012 12 variables However Conclusions complications 1 07 7 0.7 000 0.00 00 08 8 0.8 002 0.02 02 0.58 05 (0.5 2 0.2 01 0.1 0. 0.0 0.5 (0. (0 (

Abstract Background stewart-treves syndrome (STS) is an angiosarcoma associated with chronic lymphedema. Objectives This article analyses the characteristics of twenty-two patients and proposes active intervention in lymphedema and the early diagnosis of STS. Methods Twenty-two patients with STS were diagnosed at the centre over an 11-year period. Clinical manifestations, a series of conventional analyses, and histopathology were used to study these cases retrospectively. Results The age range of 22 patients with STS was 15 to 78 years. The main clinical manifestations included multiple skin and subcutaneous nodules and scattered red or purplish-red rashes in the lymphoedematous limbs. These patients often showed clinical symptoms such as lymphedema, weakness, emaciation, pain, mass, lymphadenopathy and so on. The positive rates of ultrasonography, MRI and radionuclide imaging were 66.7% (6/9), 92.3% (12/13) and 18.2% (2/11), respectively. The main points regarding active intervention in lymphedema and early diagnosis of STS were summarized. Study limitations Since this was a retrospective study, the main points summarized by the author need to be further quantified in clinical work to guide the diagnosis of this kind of disease more conveniently. In addition, further clinical trials are needed to evaluate the role of lymphedema in the occurrence and development of malignant tumors. Conclusions STS can appear in lymphoedematous tissue many years after lymphedema onset. To avoid delays in the diagnosis and therapy of STS, physicians should actively look for signs or symptoms of malignant lymphedema during the follow-up period and promptly manage patients developing problems. stewarttreves stewart treves (STS twentytwo twenty two Twentytwo Twenty 11year year 11 retrospectively 2 1 7 purplishred purplish limbs weakness emaciation pain mass on ultrasonography 667 66 66.7 6/9, 69 6/9 , 6 9 (6/9) 923 92 3 92.3 12/13 1213 12 13 (12/13 182 18 18.2 2/11, 211 2/11 (2/11) respectively conveniently addition tumors onset followup follow up problems 66. 6/ (6/9 92. 12/1 121 (12/1 18. 21 2/1 (2/11 (6/ 12/ (12/ 2/ (2/1 (6 (12 (2/ ( (1 (2

Resumen Objetivo: realizar una revisión sistemática de los estudios observacionales que analizan la asociación entre la ingesta de alimentos ultraprocesados (UPF) y el riesgo de depresión. Material y métodos: la búsqueda se adhirió a las directrices Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA); se realizó una búsqueda de estudios observacionales publicados hasta junio de 2020 en las bases de datos PubMed, Embase, Cochrane Library y Web of Science, seguida de búsquedas manuales adicionales. Ocho revisores, que trabajaron de forma independiente en equipos de dos, seleccionaron los estudios para su elegibilidad, extrajeron los datos y evaluaron el riesgo de sesgo. Los desacuerdos se resolvieron a través de la discusión o, si era necesario, a través de la adjudicación de un tercero. Se evaluaron los estudios transversales mediante la lista de comprobación metodológica de la Agency for Healthcare Research and Quality (AHRQ) y los estudios de cohortes y de casos y controles mediante la escala Newcastle-Ottawa (NOS) para la calidad. Se utilizó un formato tabular para resumir los artículos. Resultados: finalmente se seleccionaron 28 estudios que evaluaban la ingesta de UPF y el riesgo de depresión, 21 de los cuales tenían un diseño transversal, 6 un diseño de cohortes y 1 un diseño de casos y controles. De ellos, 4 estudios de cohortes y 17 estudios transversales encontraron que el consumo de UPF se asociaba positivamente con la depresión o los síntomas depresivos. Conclusiones: nuestra revisión demostró que la mayoría de los estudios incluidos en la revisión sistemática mostraron que el consumo de UPF está asociado con el riesgo de depresión. Los estudios futuros deberían considerar el uso de evaluaciones validadas del consumo de alimentos y métodos estandarizados de evaluación de la depresión para promover la comparabilidad entre los estudios.

Abstract Objective: to conduct a systematic review of the observational studies analyzing the association between ultra-processed food (UPF) intake and the risk of depression. Material and methods: the search adhered to the guidelines of Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA); a search for observational studies published until June 2020 was performed in PubMed, Embase, Cochrane Library, and Web of Science databases, followed by additional manual searches. Eight reviewers, working independently in teams of two, screened studies for eligibility, extracted data, and assessed risk of bias. We resolved disagreements through discussion or, if necessary, through adjudication by a third (LH). And the study assessed cross-sectional studies using the Agency for Healthcare Research and Quality (AHRQ) methodological checklist and cohort and case-control studies using the Newcastle-Ottawa Scale (NOS) for quality. We used a tabular format to summarize the articles. Results: twenty-eight studies evaluating UPF intake and risk of depression were finally selected, 21 of which had a cross-sectional design, 6 studies had a cohort design, and 1 had a case-control design. Of these, 4 cohort studies and 17 cross-sectional studies found that consumption of UPF were positively associated with depression or depressive symptoms. Conclusions: our review demonstrated that most studies included in the systematic review showed that UPF consumption is associated with the risk of depression. Future studies should consider the use of validated food intake assessments and standardized depression assessment methods to promote comparability between studies.

Abstract In this study, systematic pharmacological methods were used to reveal the potential pharmacological targets of baweixiaoyaosan in the treatment of major depressive disorder (MDD). We identified 133 potential active compounds through data mining and absorption, distribution, metabolism, and excretion evaluation systems. Then, the target of potential active compounds is predicted by a system model based on random forest and support vector machine methods. Next, construct herbal ingredient-target networks and target-disease networks for further analysis of multi-directional treatment methods. At the same time, we also performed gene ontology enrichment analysis, tissue location analysis, and pathway analysis on 76 potential targets. Finally, we conducted the Jun-Chen-Zuo-Shi compatibility analysis of the formula and scientifically explained the different functions of different herbs in the formula. In short, we found that the formula mainly exerts the effect of treating MDD through the four functional modules of inflammation inhibition, neuroprotection, monoamine neurotransmitter and liver. This research not only explores the mechanism of Traditional Chinese Medicine treatment of MDD from a multi-scale perspective, but also provides a reference for future research on BWXYS. It plays a role in promoting the widespread use of BWXYS. study MDD. . (MDD) 13 absorption distribution metabolism systems Then Next ingredienttarget ingredient targetdisease disease multidirectional multi directional time 7 Finally JunChenZuoShi Jun Chen Zuo Shi short inhibition neuroprotection liver multiscale scale perspective BWXYS (MDD 1

Abstract Objective The study explored improvements in pulmonary inflammation and fibrosis in a bovine type II collagen-induced rheumatoid arthritis-associated interstitial lung disease mouse model after treatment with baricitinib and the possible mechanism of action. Methods A rheumatoid arthritis-associated interstitial lung disease mouse model was established, siRNA Jak2 and lentiviral vectors were transfected with human embryonic lung fibroblast cells. And the levels of relevant proteins in mouse lung tissue and human embryonic lung fibroblasts were detected by Western blotting. Results The levels of JAK2, p-JAK2, p-STAT3, p-SMAD3, SMA, TGFβR2, FN and COL4 were increased in the lung tissues of model mice (P < 0.5) and decreased after baricitinib intervention (P < 0.05). The expression levels of p-STAT3, p-SMAD3, SMA, TGFβR2, FN and COL4 were reduced after siRNA downregulation of the JAK2 gene (P < 0.01) and increased after lentiviral overexpression of the JAK2 gene (P < 0.01). Conclusion Baricitinib alleviated fibrosis in the lung tissue of rheumatoid arthritis-associated interstitial lung disease mice, and the mechanism of action may involve the downregulation of Smad3 expression via inhibition of the Jak2/Stat3 signaling pathway, with consequent inhibition of the profibrotic effect of transforming growth factor-β1. collageninduced collagen induced arthritisassociated arthritis associated established Jak cells blotting JAK pJAK2, pJAK2 pJAK p p-JAK2 pSTAT3, pSTAT3 pSTAT STAT3, STAT3 STAT p-STAT3 pSMAD3, pSMAD3 pSMAD SMAD3, SMAD3 SMAD p-SMAD3 SMA TGFβR2 TGFβR COL P 0.5 05 0 5 0.05. 005 0.05 . 0.05) 0.01 001 01 0.01. Smad Jak2Stat3 JakStat Stat3 Stat Jak2/Stat pathway factorβ1. factorβ1 factorβ factor β1. β1 β factor-β1 p-JAK p-STAT p-SMAD 0. 00 0.0 Jak2Stat factor-β

Abstract Background Previous studies have suggested that systemic metabolic abnormalities are closely related to psoriatic arthritis (PsA). Gamma-glutamyl transpeptidase (GGT) and indirect bilirubin (IBIL), two essential active substances in hepatic metabolism that have been demonstrated as an oxidative and anti-oxidative factor respectively, have been proved to be involved in oxidative stress damage and inflammation in several human diseases. However, their role in PsA remains unclear. Methods In this retrospective comparative cohort study, a case group of 68 PsA patients and a control group of 73 healthy volunteers from the Third Hospital of Hebei Medical University were enrolled. Serum GGT, IBIL, GGT/IBIL ratio and C-reactive protein (CRP), a well applied bio-marker of systemic inflammatory in PsA, were compared between the two groups. Furthermore, the relationship of GGT, IBIL and GGT/IBIL with CRP were explored in PsA patients. Finally, the patients were divided into high inflammation group and low inflammation group according to the median value of CRP. Multivariate logistic regression analyses were used for the association of systemic inflammation level with GGT, IBIL and GGT/IBIL. Results Compared with healthy controls, PsA patients exhibited significantly higher serum GGT, GGT/IBIL, and CRP levels and lower IBIL levels. Serum GGT and GGT/IBIL were positively correlated with CRP, whereas IBIL were negatively correlated with CRP. Binary logistic regression analysis revealed that serum GGT was a risk factor for high CRP in PsA, whereas IBIL was a protective factor. Furthermore, GGT/IBIL was a better indicator of high CRP condition in PsA patients than either GGT or IBIL alone, as determined by the receiver operating characteristic curves. Conclusion GGT and IBIL may participate in the pathogenesis of PsA. Additionally, GGT, IBIL and the balance of the two may reflect systemic inflammation mediated by oxidative stress events related to metabolic abnormalities to a certain extent. . (PsA) Gammaglutamyl Gamma glutamyl (GGT , (IBIL) antioxidative anti respectively diseases However unclear study 6 7 enrolled GGTIBIL Creactive C reactive (CRP) biomarker bio marker groups Furthermore Finally controls alone curves Additionally extent (PsA (IBIL (CRP

Abstract Background: Diabetic kidney disease (DKD) is a serious microvascular complication of diabetes that affects both type 1 and type 2 diabetes patients at a high incidence rate. Naja Naja atra venom (NNAV) has been shown to have protective effects and improved renal function in diabetic rats. However, its mechanism of action is still unclear. This study aims to unravel the effectiveness and mechanisms of NNAV on DKD. Methods: We conducted in vitro experiments in which Human Kidney-2 (HK-2) cells were stimulated with high glucose, and exposed to varying concentrations of NNAV. Cell morphology, as well as α-SMA, TGF-β1, and E-cadherin levels, were analyzed using immunofluorescence and western blot. In vivo experiments involved a diabetic rat model, where varying concentrations of cobra α-neurotoxin (CTX) were administrated via gastric treatment. We observed and noted pathomorphological changes, measured biochemical and oxidative stress indices, and used western blot to assess podocin and nephrin levels. Results: High glucose levels can induce a decrease in E-cadherin expression and an increase in α-SMA and transforming growth factor-β1 (TGF-β1) expression in HK-2 cells. NNAV can inhibit the transdifferentiation of HK-2 cells to myofibroblast (MyoF) in a high glucose environment and reduce the expression of TGF-β1. Cobra α-neurotoxin (CTX) can reduce urine protein in diabetes model rats at an early stage, which is dose-independent and has a time application range. CTX can regulate the expression of nephrin and podocin. Conclusion: The present study indicates that CTX and NNAV attenuate STZ and high glucose-induced DKD. Its mechanisms of action are associated with inhibiting oxidative stress and TEMT. The study suggests that NNAV and CTX might be a potential therapeutic drug for treating DKD. Background DKD (DKD rate (NNAV However unclear Methods Kidney2 Kidney Kidney- HK2 HK (HK-2 morphology αSMA, αSMA α SMA, SMA TGFβ1, TGFβ1 TGFβ TGF β1, β1 β TGF-β1 Ecadherin E cadherin αneurotoxin neurotoxin (CTX treatment changes indices Results factorβ1 factorβ factor factor-β (TGF-β1 HK- MyoF (MyoF TGFβ1. β1. stage doseindependent dose independent range Conclusion glucoseinduced induced TEMT (HK- TGF-β (TGF-β (HK

Abstract Objectives: Lung cancer was one of the most common malignancies around the world. It has great significance in to search for the mechanism of occurrence and development of lung cancer. LIM Domain Binding protein 2 (LDB2) belongs to the LIM-domain binding family, it can be used as a binding protein that combined with other transcription factors to form the transcription complex for regulating the expression of target genes. The expression of microRNA-96-5p (miR-96-5p) has been investigated in various tumors. The aim of this study is to investigate the potential role of LDB2 and miR-96-5p in lung cancer. Methods: Real-time quantitative PCR was applied to detect the expression of LDB2 and miR-96-5p. The proliferation, invasion, and metastasis of H1299 cells were analyzed by CCK8, transwell, and wound healing assay after LDB2 or miR-96-5p transfection. Luciferase activities assay and western blot were used to reveal the targeted regulation between LDB2 and miR-96-5p. Results: Here the authors found LDB2 was down-regulated in lung cancer tissues and negatively correlated with miR-96-5p expression, it could promote or inhibit the proliferation, invasion and metastasis of H1299 cells after LDB2 knockdown or overexpression and regulate the expression of cyclinD1, MMP9, Bcl-2, and Bax via ERK1/2 signaling pathway. Furthermore, miR-96-5p exerted its function by directly binding to 3′-UTR of LDB2 and regulating expression of LDB2. miR-96-5p could promote the proliferation, invasion, and metastasis of H1299 cells. Conclusion: These findings demonstrate that LDB2 can act as a new regulator to inhibit cell proliferation, invasion, and metastasis via the ERK1/2 signaling pathway, and miR-96-5p may be a potential promising molecular by targeting LDB2 in lung cancer. Objectives world LDB (LDB2 LIMdomain domain family genes microRNA965p microRNAp microRNA 96 5p p miR965p miRp miR (miR-96-5p tumors Methods Realtime Real time miR965p. 5p. proliferation H H129 CCK8 CCK transwell transfection Results downregulated down regulated cyclinD1 cyclinD MMP9 MMP Bcl2, Bcl2 Bcl 2, Bcl-2 ERK12 ERK ERK1 ERK1/ pathway Furthermore 3′UTR 3UTR UTR 3′ 3 Conclusion (LDB 9 H12 Bcl- H1

Abstract Objective Abnormal complement activation is associated with periodontitis. W54011 is a novel non-peptide C5aR antagonist (C5aRA) that exhibits favorable anti-inflammatory effects in various inflammatory models. However, whether W54011 inhibits periodontitis has not yet been fully elucidated. To address this, we have investigated the probable anti-inflammatory mechanism of W54011 in LPS-treated inflammation in human gingival fibroblasts (HGFs). Methodology HGFs were isolated from healthy gingival tissue samples using the tissue block method and were identified with immunofluorescence staining. The CCK8 assay and reverse transcription-PCR (RT-PCR) were used to select the optimal induction conditions for Lipopolysaccharide (LPS) and C5aRA (according to supplementary data S1, S2 and S3). The levels of inflammatory cytokines, C5aR, and the activation of NF-κB/MAPK signaling pathways were determined by RT-quantitative PCR (RT-qPCR) and Western blotting. Results Immunofluorescence results showed that vimentin and FSP-1 were positive in HGFs and Keratin was negative in HGFs. Immunofluorescence staining demonstrated that C5aRA inhibited LPS-stimulated nuclear translocation of p-p65. RT-qPCR and Western blotting showed that C5aRA reduced the expression of IL-1β, IL-6, TNF-α, C5aR, p-p65, p-IκBα, p-JNK, p-c-JUN, and TLR4 in LPS-induced HGFs. Conclusion These findings suggested that C5aRA attenuated the release of inflammatory cytokines in LPS-induced HGFs by blocking the activation of the NF-κB and MAPK signaling pathways. W W5401 nonpeptide non peptide CaR C aR CaRA aRA (C5aRA antiinflammatory anti models However elucidated this LPStreated LPS treated . (HGFs) CCK transcriptionPCR transcription RTPCR RT (RT-PCR (LPS according S1 S S3. S3 S3) NFκB/MAPK NFκBMAPK NF κB/MAPK κB RTquantitative quantitative RTqPCR qPCR (RT-qPCR FSP1 FSP 1 FSP- LPSstimulated stimulated pp65. pp65 pp p p65. p65 p-p65 IL1β, IL1β ILβ IL 1β, 1β β IL-1β IL6, IL6 6, 6 IL-6 TNFα, TNFα TNF α, α TNF-α pp65, p65, pIκBα, pIκBα IκBα, IκBα p-IκBα pJNK, pJNK JNK, JNK p-JNK pcJUN, pcJUN c JUN, JUN p-c-JUN TLR LPSinduced induced NFκB W540 (HGFs κBMAPK pp6 p6 p-p6 IL- W54 p-p W5