ABSTRACT Objective: To evaluate objective treatment efficacy and safety, and subjective patient-reported outcomes in patients with complex ureteral strictures (US) undergoing minimally invasive lingual mucosal graft ureteroplasty (LMGU). Materials and Methods: We prospectively enrolled patients underwent robotic or laparoscopic LMGU between May 2020 and July 2022. Clinical success was defined as symptom-free and no radiographic evidence of re-obstruction. Patient-reported outcomes, including health-related quality of life (HRQoL), mental health status and oral health-related quality of life (OHRQoL), were longitudinally evaluated before surgery, 6 and 12 months postoperatively. Results: Overall, 41 consecutive patients were included. All procedures were performed successfully with 32 patients in robotic approach and 9 in laparoscopic. Forty (97.56%) patients achieved clinical success during the median follow-up of 29 (range 15-41) months. Although patients with complex US experienced poor baseline HRQoL, there was a remarkable improvement following LMGU. Specifically, the 6-month and 12-month postoperative scores were significantly improved compared to the baseline (p < 0.05) in most domains. Twenty-eight (68.3%) and 31 (75.6%) patients had anxiety and depression symptoms before surgery, respectively. However, no significant decrease in the incidence of these symptoms was observed postoperatively. Moreover, there was no significant deterioration of OHRQoL at 6 months and 12 months postoperatively when compared to the baseline. Conclusions: LMGU is a safe and efficient procedure for complex ureteral reconstruction that significantly improves patient-reported HRQoL without compromising OHRQoL. Assessing patients’ quality of life enables us to monitor postoperative recovery and progress, which should be considered as one of the criteria for surgical success. Objective safety patientreported patient reported (US . (LMGU) Methods 202 2022 symptomfree symptom free reobstruction. reobstruction re obstruction. obstruction re-obstruction Patientreported Patient healthrelated related , (HRQoL) OHRQoL, (OHRQoL) surgery 1 Results Overall 4 included 3 97.56% 9756 97 56 (97.56% followup follow up 2 range 1541 15 15-41 Specifically 6month month 12month p 0.05 005 0 05 domains Twentyeight Twenty eight 68.3% 683 68 (68.3% 75.6% 756 75 (75.6% respectively However Moreover Conclusions progress (LMGU 20 (HRQoL (OHRQoL 97.56 975 5 (97.56 154 15-4 0.0 00 68.3 (68.3 75.6 7 (75.6 97.5 (97.5 15- 0. 68. (68. 75. (75. 97. (97. (68 (75 (97 (6 (7 (9 (

Abstract Objective: This study aimed to develop and internally validate a prediction model for estimating the risk of spontaneous abortion in early pregnancy. Methods: This prospective cohort study included 9,895 pregnant women who received prenatal care at a maternal health facility in China from January 2021 to December 2022. Data on demographics, medical history, lifestyle factors, and mental health were collected. A multivariable logistic regression analysis was performed to develop the prediction model with spontaneous abortion as the outcome. The model was internally validated using bootstrapping techniques, and its discrimination and calibration were assessed. Results: The spontaneous abortion rate was 5.95% (589/9,895) 1. The final prediction model included nine variables: maternal age, history of embryonic arrest, thyroid dysfunction, polycystic ovary syndrome, assisted reproduction, exposure to pollution, recent home renovation, depression score, and stress score 1. The model showed good discrimination with a C-statistic of 0.88 (95% CI 0.87‒0.90) 1, and its calibration was adequate based on the Hosmer-Lemeshow test (p = 0.27). Conclusions: The prediction model demonstrated good performance in estimating spontaneous abortion risk in early pregnancy based on demographic, clinical, and psychosocial factors. Further external validation is recommended before clinical application. Objective Methods 9895 9 895 9,89 202 2022 demographics factors collected outcome techniques assessed Results 595 5 95 5.95 589/9,895 5899895 589 (589/9,895 1 variables age arrest dysfunction syndrome reproduction pollution renovation Cstatistic C statistic 088 0 88 0.8 95% (95 0.87‒0.90 087090 87 90 HosmerLemeshow Hosmer Lemeshow p 0.27. 027 0.27 . 27 0.27) Conclusions demographic application 989 89 9,8 20 59 5.9 589/9,89 589989 58 (589/9,89 08 8 0. (9 0.87‒0.9 08709 02 0.2 2 98 9, 5. 589/9,8 58998 (589/9,8 ( 0.87‒0. 0870 589/9, 5899 (589/9, 0.87‒0 087 589/9 (589/9 0.87‒ 589/ (589/ 0.87 (589 (58 (5

During the COVID-19 outbreak, there was a sharp increase in generalized anxiety disorder (GAD). Acupuncture therapy has the advantages of accurate clinical efficacy, safety and reliability, few adverse reactions, and no dependence, and is gradually becoming one of the emerging therapies for treating GAD. We present a study protocol for a randomized clinical trial with the aim of exploring the mechanism of brain plasticity in patients with GAD and evaluate the effectiveness and reliability of acupuncture treatment. Transcranial magnetic stimulation (TMS) will be used to assess cortical excitability in GAD patients and healthy people. Sixty-six GAD patients meeting the inclusion criteria will be randomly divided into two groups: TA group, (treatment with acupuncture and basic western medicine treatment) and SA group (sham acupuncture and basic western medicine treatment). Twenty healthy people will be recruited as the control group (HC). The parameters that will be evaluated are amplitude of motor evoked potentials (MEPs), cortical resting period (CSP), resting motor threshold (RMT), and Hamilton Anxiety Scale (HAMA) score. Secondary results will include blood analysis of γ-aminobutyric acid (GABA), glutamate (Glu), glutamine (Gln), serotonin (5-HT), and brain-derived nerve growth factor (BDNF). Outcomes will be assessed at baseline and after the intervention (week 8). This study protocol is the first clinical trial designed to detect differences in cerebral cortical excitability between healthy subjects and patients with GAD, and the comparison of clinical efficacy and reliability before and after acupuncture intervention is also one of the main contents of the protocol. We hope to find a suitable non-pharmacological alternative treatment for patients with GAD. COVID19 COVID 19 COVID-1 outbreak . (GAD) reactions dependence TMS (TMS Sixtysix Sixty six groups sham HC. HC (HC) MEPs, MEPs , (MEPs) CSP, CSP (CSP) RMT, RMT (RMT) HAMA (HAMA score γaminobutyric γ aminobutyric GABA, GABA (GABA) Glu, Glu (Glu) Gln, Gln (Gln) 5HT, 5HT HT 5 (5-HT) brainderived derived BDNF. BDNF (BDNF) week 8. 8 8) nonpharmacological non pharmacological COVID1 1 COVID- (GAD (HC (MEPs (CSP (RMT (GABA (Glu (Gln (5-HT (BDNF

Abstract For solving a large amount of data processing and reducing the cost to improve detection accuracy, this paper proposes an intelligent detection model for railway vehicle wheels flat. We propose an automatic data processing algorithm for labeling large-scale data to maximize the benefits of each experiment data. To improve the generalization of the detection model to improve the accuracy, we combine the vehicle dynamics simulation data and the simulation experiment data based on the single wheel rolling test bench to construct the data set for detecting the wheel flat. Different from the existing detection models, this paper provides a method for constructing the data sets, which is used to assist machine learning in constructing the intelligent detection model. The data set and its construction method can also promote the application of data mining in the field of railway transportation. This paper verified the effectiveness of the features obtained from data processing in the detection model. The data set is used to detect and identify the wheel flat and the recognition accuracy is 98.6%. largescale scale models sets transportation 986 98 6 98.6% 9 98.6 98.

ABSTRACT Introduction: This study aimed to compare the early postoperative outcomes of right anterior thoracotomy minimally invasive aortic valve replacement (RAT-MIAVR) surgery with those of median full sternotomy aortic valve replacement (MFS-AVR) approach with the goal of identifying potential benefits or drawbacks of each technique. Methods: This retrospective, observational, cohort study included 476 patients who underwent RAT-MIAVR or MFS-AVR in our hospital from January 2015 to January 2023. Of these, 107 patients (22.5%) underwent RAT-MIAVR, and 369 patients (77.5%) underwent MFS-AVR. Propensity score matching was used to minimize selection bias, resulting in 95 patients per group for analysis. Results: After propensity matching, two groups were comparable in preoperative characteristics. RAT-MIAVR group showed longer cardiopulmonary bypass time (130.24 ± 31.15 vs. 117.75 ± 36.29 minutes, P=0.012), aortic cross-clamping time (76.44 ± 18.00 vs. 68.49 ± 19.64 minutes, P=0.004), and longer operative time than MFS-AVR group (358.47 ± 67.11 minutes vs. 322.42 ± 63.84 minutes, P=0.000). RAT-MIAVR was associated with decreased hospitalization time after surgery, lower postoperative blood loss and drainage fluid, a reduced incidence of mediastinitis, increased left ventricular ejection fraction, and lower pacemaker use compared to MFS-AVR. However, there was no significant difference in the incidence of major complications and in-hospital mortality between the two groups. Conclusion: RAT-MIAVR is a feasible and safe alternative procedure to MFS-AVR, with comparable in-hospital mortality and early follow-up. This minimally invasive approach may be a suitable option for patients requiring isolated aortic valve replacement. Introduction RATMIAVR RAT MIAVR (RAT-MIAVR MFSAVR MFS AVR (MFS-AVR technique Methods retrospective observational 47 201 2023 these 10 22.5% 225 22 5 (22.5% RATMIAVR, MIAVR, 36 77.5% 775 77 (77.5% MFSAVR. AVR. bias 9 analysis Results characteristics 130.24 13024 130 24 (130.2 3115 31 15 31.1 vs 11775 117 75 117.7 3629 29 36.2 P=0.012, P0012 P P=0.012 , 0 012 P=0.012) crossclamping cross clamping 76.44 7644 76 44 (76.4 1800 18 00 18.0 6849 68 49 68.4 1964 19 64 19.6 P=0.004, P0004 P=0.004 004 P=0.004) 358.47 35847 358 (358.4 6711 67 11 67.1 32242 322 42 322.4 6384 63 84 63.8 P=0.000. P0000 P=0.000 . 000 P=0.000) fluid mediastinitis fraction However inhospital Conclusion MFSAVR, AVR, followup. followup follow up. up follow-up 4 20 202 1 22.5 2 (22.5 3 77.5 7 (77.5 130.2 1302 13 (130. 311 31. 1177 117. 362 36. P001 P=0.01 01 76.4 764 (76. 180 18. 684 6 68. 196 19. P000 P=0.00 358.4 3584 35 (358. 671 67. 3224 32 322. 638 8 63. 22. (22. 77. (77. 130. (130 P00 P=0.0 76. (76 358. (358 (22 (77 (13 P0 P=0. (7 (35 (2 (1 P=0 ( (3 P=

Abstract Objective Dizziness or vertigo in older population frequently presents in clinical settings, yet its etiology remains elusive. The objective of this study was to delineate global trends and identify frontiers in research concerning dizziness or vertigo among older population. Methods We searched the research literature published from 2003 to 2022 on older population with dizziness or vertigo using two databases from the Web of Science Core Collection. A bibliometric and visualization analysis was conducted. Bibliometric tools facilitated co-authorship, co-citation, and keyword co-occurrence analyses, encompassing countries or regions, institutions, authors, journals, and references. Results The analysis included 1322 publications authored by 6524 individuals from 2244 institutions across 67 countries or regions, spanning 92 subject categories. A steady increase in publications was noted from 2003 to 2022. The University of Munich, Harvard University, and the University of California System emerged as leading institutions with the highest publication outputs. The United States, Germany, and China were predominant in publication counts. Eva Grill was identified as the most prolific author. Otology & Neurotology and Geriatrics & Gerontology emerged as the most prolific journal and subject category, respectively. The most prevalent keywords were “dizziness”, “vertigo”, “falls”, and “geriatric”, with “management”, “gait”, and “association” recognized as the principal research hotspots. Conclusion This study provides a systematic analysis of global scientific research on older population dizziness/vertigo, revealing significant advancements in understanding over the past two decades. Management, gait, and association have emerged as the primary research focuses on recent years. These findings offer valuable insights for directing current research efforts to capture prevailing trends and explore new frontiers in this field. settings elusive 200 202 Collection conducted coauthorship, coauthorship co authorship, authorship co-authorship cocitation, cocitation citation, citation co-citation cooccurrence occurrence analyses regions authors journals references 132 652 224 6 9 categories Munich outputs States Germany counts author category respectively dizziness, , “dizziness” vertigo, “vertigo” falls, falls “falls” geriatric, geriatric “geriatric” management, management “management” gait “gait” “association hotspots dizzinessvertigo dizziness/vertigo decades Management years field 20 13 65 22 “dizziness “vertigo “falls “geriatric “management “gait 2 1

ABSTRACT Objective: MCM3AP-AS1 has been characterized as an oncogenic long non-coding RNA (lncRNA) in several cancers including papillary thyroid cancer (PTC), but its role in PTC has not been fully elucidated. Considering the critical role of lncRNAs in cancer biology, further functional analysis of MCM3AP-AS1 in PTC may provide novel insights into PTC management. Subjects and methods: Paired tumor and non-tumor tissues were collected from 63 papillary thyroid carcinoma (PTC) patients. Expression levels of MCM3AP-AS1 , miR-218 and GLUT1 in tissue samples were analyzed by qRT-PCR. Cell transfection was performed to explore the interactions among MCM3AP-AS1 , miR-218 and GLUT1 . Cell proliferation assay was performed to evaluate the effects of MCM3AP-AS1 and miR-218 on cell proliferation. Results: MCM3AP-AS1 accumulated to high levels in PTC tissues and was affected by clinical stage. MCM3AP-AS1 showed a positive correlation with GLUT1 across PTC tissues. RNA interaction prediction showed that MCM3AP-AS1 could bind to miR-218 , which can directly target GLUT1 . MCM3AP-AS1 and miR-218 showed no regulatory role regulating the expression of each other, but overexpression of MCM3AP-AS1 upregulated GLUT1 and enhanced cell proliferation. In contrast, overexpression of miR-218 downregulated GLUT1 and attenuated cell proliferation. In addition, miR-218 suppressed the role of MCM3AP-AS1 in regulating the expression of GLUT1 and cell proliferation. Conclusions: MCM3AP-AS1 may serve as a competing endogenous RNA of miR-218 to upregulate GLUT1 in PTC, thereby promoting cell proliferation. The MCM3AP-AS1/miR-218/GLUT1 pathway characterized in the present study might serve as a potential target to treat PTC. Objective MCM3APAS1 MCMAPAS MCM3AP AS1 MCM AP AS MCM3AP-AS noncoding non coding lncRNA (lncRNA elucidated biology management methods nontumor 6 (PTC patients miR218 miR 218 miR-21 GLUT qRTPCR. qRTPCR qRT PCR. PCR qRT-PCR Results stage other contrast addition Conclusions MCM3APAS1/miR218/GLUT1 MCM3APAS1miR218GLUT1 MCMAPASmiRGLUT AS1/miR 218/GLUT1 MCM3AP-AS1/miR-218/GLUT APAS MCM3APAS MCMAP miR21 21 miR-2 MCM3APAS1/miR218/GLUT MCM3APAS1miR218GLUT AS1miR ASmiR 218GLUT1 218/GLUT miR2 2 miR- 218GLUT

Abstract Objectives: Lung cancer was one of the most common malignancies around the world. It has great significance in to search for the mechanism of occurrence and development of lung cancer. LIM Domain Binding protein 2 (LDB2) belongs to the LIM-domain binding family, it can be used as a binding protein that combined with other transcription factors to form the transcription complex for regulating the expression of target genes. The expression of microRNA-96-5p (miR-96-5p) has been investigated in various tumors. The aim of this study is to investigate the potential role of LDB2 and miR-96-5p in lung cancer. Methods: Real-time quantitative PCR was applied to detect the expression of LDB2 and miR-96-5p. The proliferation, invasion, and metastasis of H1299 cells were analyzed by CCK8, transwell, and wound healing assay after LDB2 or miR-96-5p transfection. Luciferase activities assay and western blot were used to reveal the targeted regulation between LDB2 and miR-96-5p. Results: Here the authors found LDB2 was down-regulated in lung cancer tissues and negatively correlated with miR-96-5p expression, it could promote or inhibit the proliferation, invasion and metastasis of H1299 cells after LDB2 knockdown or overexpression and regulate the expression of cyclinD1, MMP9, Bcl-2, and Bax via ERK1/2 signaling pathway. Furthermore, miR-96-5p exerted its function by directly binding to 3′-UTR of LDB2 and regulating expression of LDB2. miR-96-5p could promote the proliferation, invasion, and metastasis of H1299 cells. Conclusion: These findings demonstrate that LDB2 can act as a new regulator to inhibit cell proliferation, invasion, and metastasis via the ERK1/2 signaling pathway, and miR-96-5p may be a potential promising molecular by targeting LDB2 in lung cancer. Objectives world LDB (LDB2 LIMdomain domain family genes microRNA965p microRNAp microRNA 96 5p p miR965p miRp miR (miR-96-5p tumors Methods Realtime Real time miR965p. 5p. proliferation H H129 CCK8 CCK transwell transfection Results downregulated down regulated cyclinD1 cyclinD MMP9 MMP Bcl2, Bcl2 Bcl 2, Bcl-2 ERK12 ERK ERK1 ERK1/ pathway Furthermore 3′UTR 3UTR UTR 3′ 3 Conclusion (LDB 9 H12 Bcl- H1

Abstract Objective To compare the clinical features, risk factors, distribution of Benign Paroxysmal Positional Vertigo (BPPV) subtypes, and effectiveness of canalith repositioning between geriatric and non-geriatric patients with BPPV. Methods A total of 400 patients with BPPV were enrolled. Canalith repositioning was performed according to the semicircular canals involved. Patients were divided by age into a geriatric group (≥60 years) and a non-geriatric group (20-59 years). Clinical characteristics, potential age-related risk factors, distribution of subtypes, and effectiveness of canalith repositioning were compared between the groups. Results Female sex was significantly more common in all age groups, with a peak female-to-male ratio of 5.1:1 in the group aged 50-59 years. There was a higher proportion of men in the geriatric group. A history of disease associated with atherosclerosis was significantly more common in the geriatric group (p< 0.05). Migraine was significantly more common in the non-geriatric group (p= 0.018), as was posterior canal BPPV. The horizontal canal BPPV (especially horizontal canal BPPV-cupulolithiasis), and multicanal BPPV subtypes were more common in the geriatric group, whereas anterior canal BPPV was more common in the non-geriatric group. Two canalith repositioning sessions were effective in 58.0% of the geriatric cases and in 72.6% of the non-geriatric cases (p= 0.002). There was a tendency for the effectiveness of canalith repositioning to decrease with increasing age. Conclusion BPPV was more common in women. However, the proportion of men with BPPV increased with age. Elderly patients often had a history of diseases associated with atherosclerosis (i.e., hypertension, diabetes, and hyperlipidemia). The horizontal canal BPPV (particularly horizontal canal BPPV-cupulolithiasis) and multicanal BPPV subtypes were more common and the anterior canal BPPV subtype was less common in elderly patients. The effectiveness of canalith repositioning may decrease with age. Therefore, older patients should receive more comprehensive medical treatment. Level of evidence: 4. features factors (BPPV nongeriatric non 40 enrolled involved ≥60 60 (≥6 years 2059 20 59 (20-5 . characteristics agerelated related groups femaletomale female male 5.11 511 5.1 1 5 5.1: 5059 50 50-5 p< p (p 0.05. 005 0.05 0 05 0.05) p= 0.018, 0018 0.018 , 018 0.018) especially BPPVcupulolithiasis, BPPVcupulolithiasis cupulolithiasis 580 58 58.0 726 72 6 72.6 0.002. 0002 0.002 002 0.002) women However i.e., ie i e (i.e. hypertension diabetes hyperlipidemia. hyperlipidemia hyperlipidemia) particularly BPPV-cupulolithiasis Therefore treatment evidence 4 ≥6 (≥ 205 2 (20- 11 51 5. 505 50- 00 0.0 001 0.01 01 58. 7 72. 000 0.00 i.e. (i.e ≥ ( (20 0. i.e (2

RESUMEN Introducción: El programa de entrenamiento de fuerza y acondicionamiento conocido como CrossFit ha demostrado gran capacidad de optimización en resistencia cardiorrespiratoria y muscular, fuerza, flexibilidad, potencia, velocidad, coordinación, agilidad, equilibrio y precisión. Se cree que los atletas de baloncesto pueden obtener beneficios de su práctica. Objetivo: Explorar los efectos de la práctica de CrossFit en el rendimiento deportivo de atletas de baloncesto. Métodos: Dieciséis atletas fueron divididos aleatoriamente en grupo experimental y grupo control. El grupo experimental realizó un entrenamiento adaptado basado en CrossFit, mientras que el grupo control se mantuvo con el entrenamiento tradicional. Los índices relevantes para el estudio fueron capturados antes y después de la intervención, analizados estadísticamente y discutidos teniendo en cuenta la literatura actual. Resultados: El índice medio de grasa corporal del grupo experimental se redujo del 22,33% al 17,68%, la capacidad vital media aumentó de 6210,08 ml a 6270,44 ml; el tiempo medio de la carrera de 3000 m se aceleró de 14,00 min a 12,50 min, y el valor medio de la fuerza de tracción horizontal aumentó de 63,02 kg a 79,84 kg; y el índice medio de golpeos de balón en un minuto aumentó del 31,06% al 40,17%. Conclusión: La aplicación del entrenamiento basado en CrossFit demostró mejorar eficazmente el nivel físico, la forma física y el rendimiento deportivo en jugadores de baloncesto, y puede aplicarse al entrenamiento diario. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción muscular flexibilidad potencia velocidad coordinación agilidad precisión Objetivo Métodos tradicional intervención actual Resultados 2233 22 33 22,33 1768 17 68 17,68% 621008 6210 08 6210,0 627044 6270 44 6270,4 300 1400 14 00 14,0 1250 12 50 12,5 6302 63 02 63,0 7984 79 84 79,8 3106 31 06 31,06 4017 40 40,17% Conclusión físico diario II tratamiento 223 2 3 22,3 176 1 6 17,68 62100 621 0 6210, 62704 627 4 6270, 30 140 14, 125 5 12, 630 63, 798 7 8 79, 310 31,0 401 40,17 22, 17,6 62 31, 40,1 17, 40,

RESUMO Introdução: O programa de treinamento de força e condicionamento conhecido como CrossFit tem demonstrado grande capacidade de otimização na resistência cardiorrespiratória e muscular, força, flexibilidade, potência, velocidade, coordenação, agilidade, equilíbrio e precisão. Acredita-se que os atletas de basquetebol possam obter benefícios com a sua prática. Objetivo: Explorar os efeitos da prática de CrossFit sobre o desempenho esportivo de atletas de basquetebol. Métodos: Dezesseis atletas foram divididos aleatoriamente em grupo experimental e grupo de controle. O grupo experimental executou um treinamento adaptado baseado em CrossFit, enquanto o grupo controle permaneceu com o treinamento tradicional. Os índices relevantes ao estudo foram captados antes e após a intervenção, analisados estatisticamente e discutidos considerando a literatura atual. Resultados: A taxa média de gordura corporal do grupo experimental foi reduzida de 22,33% para 17,68%, a capacidade média vital aumentou de 6210,08 ml para 6270,44 ml; o tempo médio da corrida de 3000 m foi acelerado de 14,00 min para 12,50 min, e o valor médio da força de tração horizontal foi aumentado de 63,02 kg para 79,84 kg; e a taxa média de acertos de bola em um minuto aumentou de 31,06% para 40,17%. Conclusão: A aplicação do treinamento baseado em CrossFit demonstrou melhorar efetivamente o nível físico, a aptidão física e o desempenho esportivo nos jogadores de basquetebol, podendo ser aplicado ao treinamento cotidiano. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução muscular flexibilidade potência velocidade coordenação agilidade precisão Acreditase Acredita se Objetivo Métodos tradicional intervenção atual Resultados 2233 22 33 22,33 1768 17 68 17,68% 621008 6210 08 6210,0 627044 6270 44 6270,4 300 1400 14 00 14,0 1250 12 50 12,5 6302 63 02 63,0 7984 79 84 79,8 3106 31 06 31,06 4017 40 40,17% Conclusão físico cotidiano II tratamento 223 2 3 22,3 176 1 6 17,68 62100 621 0 6210, 62704 627 4 6270, 30 140 14, 125 5 12, 630 63, 798 7 8 79, 310 31,0 401 40,17 22, 17,6 62 31, 40,1 17, 40,

ABSTRACT Introduction: The strength training and conditioning program known as CrossFit has demonstrated a great capacity for optimization in cardiorespiratory and muscular endurance, strength, flexibility, power, speed, coordination, agility, balance, and precision. It is believed that basketball athletes may derive benefits from their practice. Objective: Explore the effects of CrossFit practice on the sport performance of basketball athletes. Methods: Sixteen athletes were randomly divided into experimental and control groups. The experimental group performed adapted CrossFit-based training, while the control group remained with traditional training. The indices relevant to the study were captured before and after the intervention, statistically analyzed, and discussed considering the current literature. Results: The average body fat rate of the experimental group was reduced from 22.33% to 17.68%, the average vital capacity increased from 6210.08 ml to 6270.44 ml; the average time of the 3000 m run was accelerated from 14.00 min to 12.50 min, and the average horizontal pull force value was increased from 63.02 kg to 79.84 kg; and the average ball hit rate in one minute increased from 31.06% to 40.17%. Conclusion: The application of CrossFit-based training was shown to effectively improve the physical level, fitness, and sports performance of basketball players, and can be applied to everyday training. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction endurance flexibility power speed coordination agility balance precision Objective Methods groups CrossFitbased based intervention analyzed literature Results 2233 22 33 22.33 1768 17 68 17.68% 621008 6210 08 6210.0 627044 6270 44 6270.4 300 1400 14 00 14.0 1250 12 50 12.5 6302 63 02 63.0 7984 79 84 79.8 3106 31 06 31.06 4017 40 40.17% Conclusion level fitness players II outcomes 223 2 3 22.3 176 1 6 17.68 62100 621 0 6210. 62704 627 4 6270. 30 140 14. 125 5 12. 630 63. 798 7 8 79. 310 31.0 401 40.17 22. 17.6 62 31. 40.1 17. 40.

Abstract The seed shattering trait has been repeatedly reshaped during rice evolution. Reduced in cultivated rice and increased in weedy rice, shattering is of great agronomic importance because of its association with yield losses. Since its first descriptions, the phenotypic patterns and the genetic regulation of cultivated and weedy rice seed shattering have been extensively studied, with a variety of methods and techniques. The aim of this review is to discuss and recommend the most suitable experimental methods for phenotypic and molecular evaluation of seed shattering in cultivated and weedy rice. Rice seed shattering must be quantified, preferably, by breaking tensile strength (BTS) assays, because other methods are more prone to human errors. The evaluation time is particularly important, and the developmental stages of the panicles measured need to be recorded. QTL analyses and GWAS studies are suitable for discovery of genes influencing shattering, but the resulting genes may only be relevant in the parental lines or the populations used. The variety of cultivated rice and evolutionary origin of weedy rice accessions has a great influence on results of rice seed shattering phenotypic and genotypic analyses and needs to always be taken into account. evolution losses descriptions studied techniques quantified preferably BTS (BTS assays errors important recorded used account

Abstract Background: UVB irradiation can cause acute damage such as sunburn, or photoaging and melanoma, all of which are major health threats. Objective: This study was designed to investigate the mechanism of skin photoaging induced by UVB radiation in mice through the analysis of the differential expression of miRNAs. Methods: A UVB irradiation photoaging model was constructed. HE and Masson special stains were used to examine the modifications in the epidermis and dermis of mice. The miRNA expression profiles of the mouse skin model exposed to UVB radiation and the normal skin of mice were analyzed using miRNA-sequence analysis. GO and Pathway analysis were employed for the prediction of miRNA targets. Results: A total of 23 miRNAs were evaluated for significantly different expressions in comparison to normal skin. Among them, 7 miRNAs were up-regulated and 16 were down-regulated in the skin with photoaging of mice exposed to UVB irradiation. The differential expression of miRNA is related to a variety of signal transduction pathways, among which mmu-miR-195a-5p and mitogen-activated protein kinase (MAPK) signal pathways are crucial. There was a significant differential expression of miRNA in the skin of normal mice in comparison with the skin with photoaging induced by UVB irradiation. Study limitations: Due to time and energy constraints, the specific protein level verification, MAPK pathway exploration, and miR-195a-5p downstream molecular mechanism need to be further studied in the future. Conclusions: UVB-induced skin photoaging can be diagnosed and treated using miRNA.

Eccrine sweat glands (ESGs) perform critical functions in temperature regulation in humans. Foxa1 plays an important role in ESG maturation and sweat secretion. Its molecular mechanism, however, remains unknown. This study investigated the expression of Foxa1 and Na-K-ATPase (NKA) in rat footpads at different development stages using immunofluorescence staining, qRT-PCR, and immunoblotting. Also, bioinformatics analysis and Foxa1 overexpression and silencing were employed to evaluate Foxa1 regulation of NKA. The results demonstrated that Foxa1 was consistently expressed during the late stages of ESGs and had a significant role in secretory coil maturation during sweat secretion. Furthermore, the mRNA abundance and protein expression of NKA had similar accumulation trends to those of Foxa1, confirming their underlying connections. Bioinformatics analysis revealed that Foxa1 may interact with these two proteins via binding to conserved motifs in their promoter regions. Foxa1 gain-of-function and loss-of-function experiments in Foxa1-modified cells demonstrated that the activities of NKA were dependent on the presence of Foxa1. Collectively, these data provided evidence that Foxa1 may influence ESG development through transcriptional regulation of NKA expression.