ABSTRACT Introduction: This study aimed to compare the early postoperative outcomes of right anterior thoracotomy minimally invasive aortic valve replacement (RAT-MIAVR) surgery with those of median full sternotomy aortic valve replacement (MFS-AVR) approach with the goal of identifying potential benefits or drawbacks of each technique. Methods: This retrospective, observational, cohort study included 476 patients who underwent RAT-MIAVR or MFS-AVR in our hospital from January 2015 to January 2023. Of these, 107 patients (22.5%) underwent RAT-MIAVR, and 369 patients (77.5%) underwent MFS-AVR. Propensity score matching was used to minimize selection bias, resulting in 95 patients per group for analysis. Results: After propensity matching, two groups were comparable in preoperative characteristics. RAT-MIAVR group showed longer cardiopulmonary bypass time (130.24 ± 31.15 vs. 117.75 ± 36.29 minutes, P=0.012), aortic cross-clamping time (76.44 ± 18.00 vs. 68.49 ± 19.64 minutes, P=0.004), and longer operative time than MFS-AVR group (358.47 ± 67.11 minutes vs. 322.42 ± 63.84 minutes, P=0.000). RAT-MIAVR was associated with decreased hospitalization time after surgery, lower postoperative blood loss and drainage fluid, a reduced incidence of mediastinitis, increased left ventricular ejection fraction, and lower pacemaker use compared to MFS-AVR. However, there was no significant difference in the incidence of major complications and in-hospital mortality between the two groups. Conclusion: RAT-MIAVR is a feasible and safe alternative procedure to MFS-AVR, with comparable in-hospital mortality and early follow-up. This minimally invasive approach may be a suitable option for patients requiring isolated aortic valve replacement. Introduction RATMIAVR RAT MIAVR (RAT-MIAVR MFSAVR MFS AVR (MFS-AVR technique Methods retrospective observational 47 201 2023 these 10 22.5% 225 22 5 (22.5% RATMIAVR, MIAVR, 36 77.5% 775 77 (77.5% MFSAVR. AVR. bias 9 analysis Results characteristics 130.24 13024 130 24 (130.2 3115 31 15 31.1 vs 11775 117 75 117.7 3629 29 36.2 P=0.012, P0012 P P=0.012 , 0 012 P=0.012) crossclamping cross clamping 76.44 7644 76 44 (76.4 1800 18 00 18.0 6849 68 49 68.4 1964 19 64 19.6 P=0.004, P0004 P=0.004 004 P=0.004) 358.47 35847 358 (358.4 6711 67 11 67.1 32242 322 42 322.4 6384 63 84 63.8 P=0.000. P0000 P=0.000 . 000 P=0.000) fluid mediastinitis fraction However inhospital Conclusion MFSAVR, AVR, followup. followup follow up. up follow-up 4 20 202 1 22.5 2 (22.5 3 77.5 7 (77.5 130.2 1302 13 (130. 311 31. 1177 117. 362 36. P001 P=0.01 01 76.4 764 (76. 180 18. 684 6 68. 196 19. P000 P=0.00 358.4 3584 35 (358. 671 67. 3224 32 322. 638 8 63. 22. (22. 77. (77. 130. (130 P00 P=0.0 76. (76 358. (358 (22 (77 (13 P0 P=0. (7 (35 (2 (1 P=0 ( (3 P=

Resumo Fundamento: O antibiótico quimioterápico antraciclina doxorrubicina (DOX) pode induzir cardiotoxicidade cumulativa e levar à disfunção cardíaca. RNAs não codificantes longos (lncRNAs) podem funcionar como importantes reguladores na lesão miocárdica induzida por DOX. Objetivo: Este estudo tem como objetivo investigar o papel funcional e o mecanismo molecular do RNA antisense lncRNA OXCT1 1 (OXCT1-AS1) na lesão celular miocárdica induzida por DOX in vitro. Métodos: Cardiomiócitos humanos (AC16) foram estimulados com DOX para induzir um modelo de lesão celular miocárdica. A expressão de OXCT1-AS1, miR-874-3p e BDH1 em células AC16 foi determinada por RT-qPCR. A viabilidade das células AC16 foi medida pelo ensaio XTT. A citometria de fluxo foi empregada para avaliar a apoptose de células AC16. Western blotting foi utilizado para avaliar os níveis proteicos de marcadores relacionados à apoptose. O ensaio repórter de luciferase dupla foi conduzido para verificar a capacidade de ligação entre miR-874-3p e OXCT1-AS1 e entre miR-874-3p e BDH1. O valor de p<0,05 indicou significância estatística. Resultados: A expressão de OXCT1-AS1 foi diminuída em células AC16 tratadas com DOX. A superexpressão de OXCT1-AS1 reverteu a redução da viabilidade celular e a promoção da apoptose celular causada pela DOX. OXCT1-AS1 está ligado competitivamente ao miR-874-3p para regular positivamente o BDH1. A superexpressão de BDH1 restaurou a viabilidade das células AC16 e suprimiu a apoptose celular sob estimulação com DOX. A derrubada do BDH1 reverteu a atenuação da apoptose de células AC16 mediada por OXCT1-AS1 sob tratamento com DOX. Conclusão: LncRNA OXCT1-AS1 protege células miocárdicas humanas AC16 da apoptose induzida por DOX através do eixo miR-874-3p/BDH1. Fundamento (DOX cardíaca lncRNAs (lncRNAs Objetivo OXCT OXCT1AS1 OXCTAS AS1 AS (OXCT1-AS1 vitro Métodos AC (AC16 OXCT1AS1, AS1, miR8743p miRp miR 874 3p p BDH AC1 RTqPCR. RTqPCR RT qPCR. qPCR RT-qPCR XTT OXCT1-AS p005 0 05 p<0,0 estatística Resultados Conclusão miR8743p/BDH1. miR8743pBDH1 miRpBDH 3p/BDH1. miR-874-3p/BDH1 OXCT1AS (OXCT1-AS (AC1 87 p00 p<0, miR8743p/BDH1 pBDH miR8743pBDH 3pBDH1 3p/BDH1 miR-874-3p/BDH (AC 8 p0 p<0 miR8743p/BDH 3pBDH 3p/BDH p<

Abstract Background: The anthracycline chemotherapeutic antibiotic doxorubicin (DOX) can induce cumulative cardiotoxicity and lead to cardiac dysfunction. Long non-coding RNAs (lncRNAs) can function as important regulators in DOX-induced myocardial injury. Objective: This study aims to investigate the functional role and molecular mechanism of lncRNA OXCT1 antisense RNA 1 (OXCT1-AS1) in DOX-induced myocardial cell injury in vitro. Methods: Human cardiomyocytes (AC16) were stimulated with DOX to induce a myocardial cell injury model. OXCT1-AS1, miR-874-3p, and BDH1 expression in AC16 cells were determined by RT-qPCR. AC16 cell viability was measured by XTT assay. Flow cytometry was employed to assess the apoptosis of AC16 cells. Western blotting was used to evaluate protein levels of apoptosis-related markers. Dual-luciferase reporter assay was conducted to verify the binding ability between miR-874-3p and OXCT1-AS1 and between miR-874-3p and BDH1. The value of p<0.05 indicated statistical significance. Results: OXCT1-AS1 expression was decreased in DOX-treated AC16 cells. Overexpression of OXCT1-AS1 reversed the reduction of cell viability and promotion of cell apoptosis caused by DOX. OXCT1-AS1 is competitively bound to miR-874-3p to upregulate BDH1. BDH1 overexpression restored AC16 cell viability and suppressed cell apoptosis under DOX stimulation. Knocking down BDH1 reversed OXCT1-AS1-mediated attenuation of AC16 cell apoptosis under DOX treatment. Conclusion: LncRNA OXCT1-AS1 protects human myocardial cells AC16 from DOX-induced apoptosis via the miR-874-3p/BDH1 axis. Background (DOX dysfunction noncoding non coding lncRNAs (lncRNAs DOXinduced induced Objective OXCT OXCT1AS1 OXCTAS AS1 AS (OXCT1-AS1 vitro Methods AC (AC16 model OXCT1AS1, AS1, miR8743p, miR8743p miRp miR 874 3p, 3p p BDH AC1 RTqPCR. RTqPCR RT qPCR. qPCR RT-qPCR apoptosisrelated related markers Dualluciferase Dual luciferase OXCT1-AS p005 0 05 p<0.0 significance Results DOXtreated treated stimulation OXCT1AS1mediated OXCTASmediated mediated treatment Conclusion miR8743p/BDH1 miR8743pBDH1 miRpBDH 3p/BDH1 miR-874-3p/BDH axis OXCT1AS (OXCT1-AS (AC1 87 p00 p<0. miR8743p/BDH pBDH miR8743pBDH 3pBDH1 3p/BDH (AC 8 p0 p<0 3pBDH p<

ABSTRACT Objective: MCM3AP-AS1 has been characterized as an oncogenic long non-coding RNA (lncRNA) in several cancers including papillary thyroid cancer (PTC), but its role in PTC has not been fully elucidated. Considering the critical role of lncRNAs in cancer biology, further functional analysis of MCM3AP-AS1 in PTC may provide novel insights into PTC management. Subjects and methods: Paired tumor and non-tumor tissues were collected from 63 papillary thyroid carcinoma (PTC) patients. Expression levels of MCM3AP-AS1 , miR-218 and GLUT1 in tissue samples were analyzed by qRT-PCR. Cell transfection was performed to explore the interactions among MCM3AP-AS1 , miR-218 and GLUT1 . Cell proliferation assay was performed to evaluate the effects of MCM3AP-AS1 and miR-218 on cell proliferation. Results: MCM3AP-AS1 accumulated to high levels in PTC tissues and was affected by clinical stage. MCM3AP-AS1 showed a positive correlation with GLUT1 across PTC tissues. RNA interaction prediction showed that MCM3AP-AS1 could bind to miR-218 , which can directly target GLUT1 . MCM3AP-AS1 and miR-218 showed no regulatory role regulating the expression of each other, but overexpression of MCM3AP-AS1 upregulated GLUT1 and enhanced cell proliferation. In contrast, overexpression of miR-218 downregulated GLUT1 and attenuated cell proliferation. In addition, miR-218 suppressed the role of MCM3AP-AS1 in regulating the expression of GLUT1 and cell proliferation. Conclusions: MCM3AP-AS1 may serve as a competing endogenous RNA of miR-218 to upregulate GLUT1 in PTC, thereby promoting cell proliferation. The MCM3AP-AS1/miR-218/GLUT1 pathway characterized in the present study might serve as a potential target to treat PTC. Objective MCM3APAS1 MCMAPAS MCM3AP AS1 MCM AP AS MCM3AP-AS noncoding non coding lncRNA (lncRNA elucidated biology management methods nontumor 6 (PTC patients miR218 miR 218 miR-21 GLUT qRTPCR. qRTPCR qRT PCR. PCR qRT-PCR Results stage other contrast addition Conclusions MCM3APAS1/miR218/GLUT1 MCM3APAS1miR218GLUT1 MCMAPASmiRGLUT AS1/miR 218/GLUT1 MCM3AP-AS1/miR-218/GLUT APAS MCM3APAS MCMAP miR21 21 miR-2 MCM3APAS1/miR218/GLUT MCM3APAS1miR218GLUT AS1miR ASmiR 218GLUT1 218/GLUT miR2 2 miR- 218GLUT

Abstract Heigen is the root and rhizomes of Duhaldea Nervosa Anderberg., a traditional medicine food homology herb used to treat rheumatoid arthritis in China,but the potential active components and the underlying mechanism have not been clarified. In this study, the chemical compounds and the components absorbed into the blood from Heigen were assessed using ultra-high performance liquid chromatography coupled with quadrupole Orbitrap high-resolution mass spectrometry. 72 chemical constituents were identified in Heigen, 13 blood prototypical constituents, and 9 metabolites were found in serum samples after intragastric administration of Heigen extracts to rats. Then, using network pharmacology and molecule docking, 10 compounds were assumed to be potentially key active compounds against RA, which may be via the multi-component, multi-target and multi-pathway interaction mechanism of Heigen in the treatment of RA, and also provides a pharmacological basis for the treatment of RA. Anderberg Anderberg. Chinabut China but clarified study ultrahigh ultra high highresolution resolution spectrometry 7 1 rats Then docking RA multicomponent, multicomponent multi component, component multi-component multitarget target multipathway pathway

Abstract Perianal fistula is a common complication of Crohn disease, and it is a great burden on the life and psychology of patients, but its treatment is still a difficult problem to face. In recent years, progress in the treatment of Crohn disease has progressed rapidly due to the advent of biological agents, but there has been a lack of research on perianal fistula in Crohn disease, and the direction of research has been scattered; therefore, the author reviews the traditional treatment of perianal fistula in Crohn disease in the context of the available literature and discusses emerging and potential therapeutic approaches. patients face years agents scattered therefore approaches

Abstract Objectives Metastasis is one of the biggest challenges in the management of Esophageal Squamous Cell Carcinoma (ESCC), of which molecular mechanisms remain elusive. The present study aimed to explore the roles and underlying mechanisms of Transmembrane protein 26 (TMEM26) in ESCC. Method TMEM26 expressions in tumorous and adjacent tissues from patients with ESCC and in normal esophageal epithelial and ESCC cell lines were detected by immunostaining and western blotting, respectively. The Epithelial-Mesenchymal Transition (EMT), a critical process during metastasis, was investigated by wound healing and Transwell assays, and EMT-related proteins were examined after the TMEM26 alteration in ESCC cell lines. NF-κB signaling activation and Tight Junction (TJ) protein expression were analyzed by western blotting and immunofluorescence, respectively. In vivo verification was performed on the liver metastatic murine model. Results Compared with non-cancerous esophageal tissues and cells, the TMEM26 expression level was higher in ESCC samples and cell lines, where the plasma membrane localization of TMEM26 was observed. The EMT-related processes of ESCC cells were suppressed by RNAi depletion of TMEM26 but aggravated by TMEM26 overexpression. Mechanistically, TMEM26 promoted NF-κB signaling to accelerate EMT in ESCC cells. The plasma membrane presentation and assembly of TJ proteins were impaired by TMEM26. Conclusion Overall, TMEM26 acts as a critical determinant for EMT in ESCC cells by disrupting TJ formation and promoting NF-κB signaling, which may be a potential therapeutic target for treating metastatic ESCC. ESCC, , (ESCC) elusive 2 TMEM (TMEM26 TMEM2 respectively EpithelialMesenchymal Epithelial Mesenchymal EMT, (EMT) metastasis assays EMTrelated related NFκB NF κB (TJ immunofluorescence model noncancerous non cancerous observed overexpression Mechanistically Overall (ESCC (TMEM2 (EMT (TMEM

ABSTRACT Objective: To determine the absolute number of serum T lymphocytes and cytokine levels and the characteristics of patients with active pulmonary tuberculosis and to assess their effect on the immune status of these patients and their diagnostic and predictive value for tuberculosis. Methods: We included 1,069 patients with active tuberculosis, 51 patients with latent tuberculosis infection, and 600 health individuals. Absolute serum T-lymphocyte counts and cytokine levels were quantified. Results: T lymphocytes were significantly reduced in patients with active tuberculosis when compared with healthy individuals. The immune function of patients gradually decreased with age and was stronger in female patients than in males. Th1 cells expressed higher levels of cytokines than did Th2 cells. Logistic regression analysis showed that reduced CD3+ T, CD8+ T, and NK cell counts, as well as reduced IL-4 and IFN-g expression, were independent influencing factors for active tuberculosis. ROC analysis showed that the sensitivity and specificity of absolute CD3+ T and CD8+ T lymphocyte counts and combined factors were significantly higher than were those of IL-4 and IFN-g for diagnosing active tuberculosis. Conclusions: Serum T-lymphocyte counts and cytokine levels can assess the immune status of tuberculosis patients; they are also useful biomarkers for predicting and diagnosing tuberculosis. Objective Methods 1069 1 069 1,06 5 infection 60 individuals Tlymphocyte quantified Results males Th CD3 CD CD8 IL4 IL 4 IL- IFNg IFN g expression Conclusions 106 06 1,0 6 10 0 1,

RESUMO Objetivo: Determinar o número absoluto de linfócitos T séricos e os níveis de citocinas séricas, bem como as características, de pacientes com tuberculose pulmonar ativa e avaliar o efeito desses no estado imunológico desses pacientes e seu valor diagnóstico e preditivo para tuberculose. Métodos: Foram incluídos 1.069 pacientes com tuberculose ativa, 51 pacientes com tuberculose latente e 600 indivíduos saudáveis. Foram realizadas a contagem absoluta de linfócitos T séricos e a quantificação de citocinas séricas. Resultados: Os linfócitos T estavam significativamente reduzidos nos pacientes com tuberculose ativa em comparação com os indivíduos saudáveis. A função imunológica dos pacientes diminuiu gradativamente com a idade e mostrou-se mais forte nas mulheres do que nos homens. As células Th1 expressaram maiores níveis de citocinas do que as células Th2. A análise de regressão logística mostrou que contagens reduzidas de células T CD3+, T CD8+ e NK e expressão reduzida de IL-4 e IFN-g foram fatores de influência independentes para tuberculose ativa. A análise ROC mostrou que a sensibilidade e especificidade dos valores absolutos de linfócitos T CD3+ e T CD8+ e de fatores combinados foram significativamente maiores do que as da IL-4 e do IFN-g para o diagnóstico da tuberculose ativa. Conclusões: A contagem de linfócitos T séricos e os níveis de citocinas séricas podem avaliar o estado imunológico de pacientes com tuberculose; também são biomarcadores úteis na predição e diagnóstico da tuberculose. Objetivo características Métodos 1069 1 069 1.06 5 60 saudáveis Resultados mostrouse se homens Th Th2 CD3 CD CD8 IL4 IL 4 IL- IFNg IFN g Conclusões 106 06 1.0 6 10 0 1.

RESUMEN Introducción Al ser un deporte físico de alta intensidad, el atletismo puede provocar fácilmente graves lesiones físicas e incluso problemas psicológicos si no se diagnostican a tiempo tras la fatiga deportiva. Objetivo Estudiar la relación entre la fatiga deportiva y la intervención nutricional en el atletismo. Métodos Se investigó a 100 practicantes de atletismo de una provincia. Mediante un estudio bibliográfico, un cuestionario y otras pruebas de datos, se analizó la fatiga deportiva y el estado nutricional de los atletas para averiguar la relación necesaria entre la fatiga deportiva y la intervención nutricional. Resultados El juicio del entrenador sobre la fatiga deportiva durante el entrenamiento de los atletas se caracteriza por la falta de precisión técnica, lo que conduce a la disminución de la capacidad de recuperación física de los atletas y agrava la fatiga deportiva en cierta medida. Como ejercicio físico de alta intensidad, el atletismo necesita un diagnóstico de la fatiga deportiva no sólo a tiempo, sino también una intervención nutricional eficaz. Conclusión La investigación muestra que, tras un ejercicio de alta intensidad, el diagnóstico de la fatiga deportiva de los atletas de atletismo es de gran importancia para juzgar la función física de los atletas, y la intervención nutricional científica desempeña un papel importante en la función física excesiva de los atletas, mejorando su capacidad deportiva y aliviando la fatiga deportiva. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. intensidad 10 provincia bibliográfico datos técnica medida eficaz II tratamiento 1

ABSTRACT Introduction As a high-intensity physical sport, athletics can easily cause serious physical injuries and even psychological problems if not diagnosed after sports fatigue. Objective Study the relationship between sports fatigue and nutritional intervention in track and field. Methods One hundred track and field athletes in a province were investigated. Through literature survey, questionnaire survey, and other data testing, track and field athletes’ sports fatigue and nutritional status were analyzed to determine the necessary relationship between sports fatigue and nutritional intervention. Results The coach’s judgment of sports fatigue during athletes’ training is characterized by a lack of technical accuracy, leading to the decline of athletes’ physical recovery ability and aggravating sports fatigue to a certain extent. As a high-intensity physical exercise, athletics must diagnose sports fatigue in time and provide effective nutritional intervention. Conclusion The research shows that after high-intensity exercise, the diagnosis of sports fatigue in track and field athletes is of great importance in judging the athletes’ physical function, and scientific nutritional intervention plays an important role in athletes’ excessive physical function, improving their sports ability and relieving sports fatigue. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. highintensity high intensity sport investigated survey testing coachs coach s accuracy extent exercise function II outcomes

RESUMO Introdução Por ser um esporte físico de alta intensidade, o atletismo pode facilmente causar lesões físicas graves e até mesmo problemas psicológicos se não forem diagnosticáveis a tempo após a fadiga esportiva. Objetivo Estudar a relação entre a fadiga esportiva e a intervenção nutricional no atletismo. Métodos Foram investigados 100 praticantes de atletismo em uma província. Através de pesquisa bibliográfica, levantamento de questionários e outros testes de dados, a fadiga esportiva e o estado nutricional dos esportistas de atletismo foram analisados para descobrir a relação necessária entre a fadiga esportiva e a intervenção nutricional. Resultados O julgamento do treinador sobre a fadiga esportiva durante o treinamento dos atletas é caracterizado pela falta de precisão técnica, levando ao declínio da capacidade de recuperação física dos atletas e agravando a fadiga esportiva em certa proporção. Como um exercício físico de alta intensidade, o atletismo necessita de diagnóstico da fadiga esportiva não apenas no tempo, mas também de uma intervenção nutricional eficaz. Conclusão A pesquisa mostra que após o exercício de alta intensidade, o diagnóstico da fadiga esportiva dos praticantes de atletismo é de grande importância para julgar a função física dos atletas, e a intervenção nutricional científica desempenha um papel importante na função física excessiva dos atletas, melhorando sua capacidade esportiva e aliviando a fadiga esportiva. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. intensidade 10 província bibliográfica dados técnica proporção eficaz II tratamento 1

RESUMEN Introducción: La calidad de la fuerza puede dividirse en fuerza total, fuerza rápida, fuerza a la resistencia y fuerza de reacción, entre las cuales la fuerza rápida es un tema suficientemente discutido en las investigaciones de la industria del deporte durante los últimos años. Objetivo: Estudiar las repercusiones del entrenamiento excéntrico de los miembros inferiores en la capacidad de salto de los deportistas de fútbol. Métodos: Se utilizó un dispositivo de entrenamiento centrífugo multifuncional como herramienta de entrenamiento, y se analizó el efecto del entrenamiento excéntrico sobre la fuerza rápida en jugadores de fútbol. Resultados: La fuerza media de los futbolistas del grupo experimental aumentó en unos 524N, y la tasa de finalización aumentó en un 76%. Tanto la fuerza media como la tasa de finalización mejoraron significativamente. En comparación con el grupo de control, el grupo experimental obtuvo un mayor beneficio del índice de potencia explosiva y del índice cinético. También hubo una mejora significativa en los cuatro índices de capacidad de salto, los tres índices de sensibilidad (excepto la frecuencia de paso de 15s) y el entrenamiento excéntrico en el grupo experimental (P < 0,01) Conclusión: El entrenamiento excéntrico de las extremidades inferiores propuesto tuvo un efecto beneficioso en la capacidad de salto de los atletas. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción total reacción años Objetivo fútbol Métodos Resultados 524N N 76 76% significativamente control cinético excepto 15s s P 0,01 001 0 01 Conclusión atletas II tratamiento 7 0,0 00 0,

ABSTRACT Introduction: Strength quality can be divided into total strength, rapid strength, endurance strength, and reaction strength, among which rapid strength has been a much-discussed theme in sports industry research. Objective: Study the impacts of eccentric lower limb training on jumping ability in soccer athletes. Methods: A multifunctional centrifugal training device was used as a training tool, and the effect of eccentric training on rapid strength in soccer players was analyzed. Results: The average force of soccer players in the experimental group increased by about 524N, and the completion rate increased by about 76%. Both average strength and completion rate were significantly improved. Compared to the control group, the experimental group benefited more from the explosive power and kinetic indexes. There was also a significant improvement in the four indices of jumping ability, the three indices of sensitivity (except 15s step frequency), and eccentric training in the experimental group (P < 0.01). Conclusion: The proposed lower limb eccentric training resulted in a beneficial effect on the jumping ability of athletes. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction muchdiscussed much discussed research Objective athletes Methods tool analyzed Results 524N N 76 76% improved indexes except s frequency, frequency , frequency) P 0.01. 001 0.01 . 0 01 0.01) Conclusion II outcomes 7 00 0.0 0.

RESUMO Introdução: A qualidade da força pode ser dividida em força total, força rápida, força à resistência e força de reação, entre as quais a força rápida é um tema bastante discutido nas pesquisas da indústria esportiva pelos últimos anos. Objetivo: Estudar os impactos do treinamento excêntrico dos membros inferiores sobre a capacidade de salto nos atletas de futebol. Métodos: Foi utilizado um dispositivo de treinamento centrífugo multifuncional como instrumento de treino, analisou-se o efeito do treinamento excêntrico sobre a força rápida em jogadores de futebol. Resultados: A força média dos jogadores de futebol do grupo experimental aumentou em cerca de 524N, e a taxa de conclusão aumentou em cerca de 76%. Tanto a força média quanto a taxa de finalização foram significativamente aprimoradas. Em comparação com o grupo controle, o grupo experimental teve um benefício maior do índice de potência explosiva e índice cinético. Também houve melhora significativa nos quatro índices de habilidade de salto, nos três índices de sensibilidade (exceto a frequência de passos de 15s), e no treinamento excêntrico do grupo experimental (P < 0,01). Conclusão: O treinamento excêntrico proposto para membros inferiores resultou em um efeito benéfico sobre a capacidade de salto dos atletas. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução total reação anos Objetivo Métodos treino analisouse analisou se Resultados 524N N 76 76% aprimoradas controle cinético exceto 15s, 15s s , 15s) P 0,01. 001 0,01 . 0 01 0,01) Conclusão II tratamento 7 00 0,0 0,

Abstract Anthocyanin/polyvinyl alcohol/chitosan composite membrane was prepared by solution casting method using chitosan (CS) and polyvinyl alcohol (PVA) as matrix and natural pigment anthocyanin as filler. The structures of the composite films were characterized by Uv-visible reflectance spectrum (UV-VIS), Fourier infrared spectroscopy (FT-IR) and the scanning electron microscopy (SEM). The effects of adding anthocyanins on the physical properties, water resistance, degradation, antibacterial properties and pH responsiveness of the films were investigated. The experimental results showed that the prepared anthocyanin/polyvinyl alcohol/chitosan composite indicator films show color changes from pink to purple to blue and then to yellow-green when the pH value ranges from 2 to 14. The total color difference ΔE value is greater than 12. The antibacterial properties and other properties of the composite film can enhance by adding anthocyanin. The tensile strength of anthocyanin/polyvinyl alcohol/chitosan film is more than 70 MPa, which is larger than that of polyvinyl film and polyvinyl alcohol/chitosanfilm. The film has excellent physical, antibacterial properties and obvious pH response performance, making it ideal for food packaging.

Abstract Clinically, methane is an anti-inflammatory gas used to treat organ damage. There is no extensive research on methane's repair of traumatic brain injury (TBI), so this study aims to investigate the role of methane and its potential mechanism. A rat TBI model was established by mechanical impact. SD rats were divided into four groups, including sham group, TBI group, 2 or 20 mL/kg methane-rich saline (MS) group. Morris water maze test revealed that MS improved cognitive function in TBI rats. HE and Nissl staining showed that MS reduced the blurring of brain tissues boundary, the number of necrotic plaques and neurons in TBI rats. In addition, ELISA revealed that MS restored the oxidative stress of TBI rats and reversed the inflammatory effect of TBI on brain cells, and TUNEL staining detected that MS inhibited cell apoptosis. Western blotting showed that MS downregulated Caspase-3 and Bax protein levels and upregulated Bcl-2 level. And MS inhibited phosphorylated expressions of Janus kinase 1 (JAK1), signal transducer and activator of the transcription (STAT1) and NF-κb-p65. In conclusion, MS improved cognitive function, reduced inflammatory response and oxidative stress and inhibited cell apoptosis to relieve rat TBI by blocking the activation of the JAK1/STAT1/NF-κb-p65 pathway.

Abstract Objective To explore the molecular mechanism of neuropathologic damage induced by radiofrequency ablation at different temperatures. Methods This is basic research, and 36 SD rats were used to construct the neuropathological injury model. The rats were subjected to radiofrequency stimulation at different temperatures and were divided into 6 groups according to the temperature injury: 42°, 47°, 52°, 57°, 62°, and 67°C groups. Conduction time, conduction distance, and nerve conduction velocity were recorded after temperature injury. HE-staining was used to observe the histopathological morphology of the sciatic nerve. The expression of SCN9A, SCN3B, and NFASC protein in sciatic nerve tissue were detected by western blot. Results With the increase in temperature, nerve conduction velocity gradually decreased, and neurons were damaged when the temperature was 67°C. HE-staining showed that the degrees of degeneration of neurons in rats at 47°, 52°, 57°, 62°, and 67°C were gradually increased. The expression of SCN9A, SCN3B protein in 57°, 62°, 67°C groups were much higher than that of NC, 42°, 47°, 52°C groups. However, the expression of NFASC protein in 57°, 62°, 67°C groups was much lower than that of the NC, 42°, 47°, 52°C groups. Conclusion There was a positive correlation between temperature caused by the radiofrequency stimulation to neuropathological damage. The mechanism is closely related to the expression of SCN9A, SCN3B, and NFASC protein in nerve tissue caused by heat transfer injury.

Abstract Melatonin is known to influence various physiological processes, including plant ripening; however, the effect of melatonin on postharvest blueberry fruit softening is unknown. Herein, the effects of melatonin at different concentrations (100, 300, and 500 μmol L-1) on the quality parameters, cell wall substances, cell-wall-degrading enzymes, and cell wall ultrastructure of blueberry fruits were investigated during low-temperature storage. Melatonin treatment was found to effectively delay the decline in fruit firmness, slow the loss of soluble solids and titratable acids, and decrease the respiration rate and decay rate of blueberry fruits. The blueberry fruits treated with 300 μmol L-1 melatonin exhibited the best quality. Melatonin treatment promoted anthocyanin accumulation, delayed ascorbic acid degradation, maintained a low relative membrane permeability in pulp, inhibited increases in lipoxygenase (LOX) activity, and reduced membrane lipid peroxidation. Moreover, melatonin controlled the formation of soluble pectin by inhibiting the activities of pectin methylesterase (PME), polygalacturonase (PG), cellulase (Cx), and β-glucosidase (β-Glu) while maintaining high contents of protopectin and cellulose. These results indicate that melatonin can effectively improve blueberry fruit quality and antioxidant capacity as well as and delay fruit softening by inhibiting the degradation of cell wall substances.

Abstract Glutathione S-transferases (GSTs) are proteins synthesized in plants and responsible for their tolerance to environmental stresses. However, little information is available on the GST gene family of sweet potato, a globally important crop. The genetic evolution of GSTs in sweet potato remains unclear. The present study investigated the GST gene family in sweet potato by transcriptomic and comparative genomic analyses. A total of 51 GSTs were identified. Gene expression analysis showed differential expression patterns of the GSTs between two investigated varieties. Some GST expression levels were either up- or downregulated under oxidative, salinity and drought stresses. The results of the investigation provided new insights on the GST gene family in sweet potato, which may further the understanding of the roles of these genes in regulating abiotic stresses.