Abstract The effectiveness and feasibility of a pork kidney lavage method to remove heavy metals and antibiotics from fresh pork kidney were investigated. This work provides a basis for the research and development of contaminant-free pork kidney foods. A comparative study was performed on pork kidneys with traditional treatment versus lavage treatment. The contents of Cu, Pb and Hg, 18 sulfonamides, 15 quinolones, ampicillin and cefalexin were detected by FAAS, GF-AAS, AFS and LC-MS/MS. The detection results of heavy metals and antibiotics showed that the content of Cu in pork kidney after lavage was significantly reduced, and antibiotics such as sulfachlorpyridazine and enrofloxacin were not detected. Therefore, lavage is an effective method to remove toxins from fresh pork kidney and can remove heavy metals and antibiotics to the maximum extent, providing a basic guarantee for the production of contaminant-free pork kidney foods.

Abstract Salted duck egg white was desalted and hydrolyzed to produce angiotensin I-converting enzyme (ACE) inhibitory peptides. Single factor test and response surface design were performed to determine the best hydrolysis conditions: enzyme dosage 11875.99 U/g, substrate concentration 33.04 g/L and hydrolysis time 4 h. The fraction V (MW < 1 kDa), which exhibited the strongest ACE inhibitory activity, was characterized by HPLC-ESI-MS/MS. Eighty-three peptides were identified, and among them Ile-Leu-Lys-Pro, Ile-Asn-Ser-Trp, Ile-Arg and His-Pro-Ala were synthesized for further research. Ile-Leu-Lys-Pro exhibited the highest ACE inhibitory activity (IC50: 0.355 mM). The molecular docking studies revealed that nine amino acids contributed to stabilize the docking complex. The ACE inhibition of Ile-Leu-Lys-Pro and Ile-Asn-Ser-Trp were mainly attributed to Ile in N-terminal. The residues Glu362 and Ala332 were the important binding sites in molecular docking. This research expands the understanding of ACE inhibitory peptides from duck egg white as well as highlights an opportunity for recycling an otherwise discarded byproduct.

Abstract A simple and selective liquid chromatography tandem with mass spectrometry (LC-MS/ MS) method for quantification of lobetyolin in rat plasma was developed and validated. Chromatographic separation was achieved on a Thermo ODS C18 reversed-phase column using 0.1% aqueous formic acid-methanol (50:50, v/v) in an isocratic elution mode at a flow rate of 0.4 mL.min-1. LC/MS performance was done in a positive ion ESI mode and the MS/MS transitions were monitored at m/z 419.3 [M+Na]+ → m/z 203.1 for lobetyolin and m/z 394.9 [M+Na]+ → m/z 231.9 for IS, respectively. The assay exhibited a linear dynamic range over 1.0-500 ng.mL-1 for lobetyolin in plasma. Both the precision (%RSD) and accuracy (RE%) were within acceptable criteria (<15%). Recoveries ranged from 87.0% to 95.6%, and the matrix effects were from 91.0% to 101.3%. After oral administration, the peak plasma concentration of lobetyolin was obtained as 60.1 ng.mL-1 at 1.0 h. The proposed LC-MS/MS method could be applied to a pharmacokinetic study employing 66 samples from 6 Wistar rats.

RESUMO OBJETIVO Comparar o efeito de dois métodos de fixação interna no tratamento de fraturas da clavícula proximal. MÉTODOS Cinquenta pacientes com fraturas da clavícula proximal receberam tratamento cirúrgico. Eles foram divididos em um grupo de placa T clavicular e um grupo de miniplacas duplas. A duração da operação, perda de sangue durante a operação, tempo de cura da fratura e infecção na incisão foram avaliados nos dois grupos. RESULTADOS O tempo de operação (t=2,063, P=0,058), perda de sangue durante a operação (t=1,979, P=0,062) e tempo de cura das fraturas (t=1,082, P=0,066) não foram estatisticamente significativos nos dois grupos. Os pacientes foram acompanhados por 12-18 meses; um dos pacientes do grupo da placa T teve retirada antecipada dos parafusos, mas não apresentou sintomas clínicos. Aos dois meses de acompanhamento, a pontuação ASES no grupo de miniplacas duplas foi significativamente melhor do que a do grupo de placas T (P<0,001). Porém, no acompanhamento de seis meses, uma semana antes da remoção da fixação interna e do acompanhamento final, os dois grupos não apresentavem diferenças significativas (P>0,05). CONCLUSÃO Ambas técnicas de fixação interna têm resultados clínicos semelhantes quanto a duração da operação, perda de sangue durante a operação e tempo de cura da fratura. A fixação de miniplacas duplas apresenta vantagens quanto a redução das complicações e cura mais rápida da fratura, sendo, portanto, um método mais eficaz para tratar fraturas da clavícula proximal.

SUMMARY OBJECTIVE To compare the effect of two internal fixation methods in the treatment of proximal clavicle fractures. METHODS Fifty patients with proximal clavicle fractures received surgical treatment. They were divided into a clavicular T-plate group and a double mini-plates group. The duration of the operation, blood loss during the operation, fracture healing time, and incision infection were evaluated between the two groups. RESULTS Operation time (t=2.063, P=0.058), intraoperative bleeding (t=1.979, P=0.062), and fracture healing time (t=1.082, P=0.066) were not statistically significant in the two groups. The patients were followed up for 12-18 months; one patient in the T-plate group had early removal of nails, but no clinical symptoms. At the 2-month follow-up, the ASES score in the double mini-plates group was significantly better than in the T-plate group (P<0.001); but at the 6-month follow-up, 1-week before removal of internal fixation and the final follow-up, the two groups had no significant differences (P>0.05). CONCLUSIONS Both internal fixations have similar clinical results in the duration of operation, blood loss during the operation, and fracture healing time. The double mini-plates fixation presents advantages by reducing complications and speeding fracture healing; thus it is a more effective method to treat proximal clavicle fractures.

Abstract Purpose Glutamine, as an essential part of enteral nutrition and parenteral nutrition agent, has been widely recognized to be a kind of important intestinal mucosa protectant in clinical practice and experimental research. However, the mechanisms of its protective effects are still not fully understand. Consequently, this study aimed to explore the potential mechanism of glutamine on ischemia-reperfusion (I/R) injury induced endoplasmic reticulum (ER) stress in intestine. Methods An experimental model of intestinal I/R in rats was established by 1 hour occlusion of the superior mesenteric artery followed by 3 hours of reperfusion. Morphologic changes of intestinal mucosa, apoptosis of epithelial cells, and expression of intestinal Grp78, Gadd153, Caspase-12, ATF4, PERK phosphorylation (P-PERK) and elF2αphosphorylation(P-elF2α) were determined. Results After I/R, the apoptotic index of intestinal mucosa epithelial cells observably increased with notable necrosis of intestinal mucosa, and the expressions of Grp78, Gadd153, Caspase-12, ATF4, P-PERK and P-elF2αall were increased. However, treatment with glutamine could significantly relieve intestinal I/R injury and apoptosis index. Moreover, glutamine could clearly up-regulate the expression of Grp78, restrain P-PERK and P-elF2α, and reduce ATF4, Gadd153 and Caspase-12 expressions. Conclusion Glutamine may be involved in alleviating ER stress induced intestinal mucosa cells apoptosis.

Abstract Circulating miRNAs presenting in plasma in a stable manner have been demonstrated their potential role as a promising biomarkers in many human diseases, such as Alzheimer’s disease, melanoma and ovarian carcinoma. However, few circulating miRNAs could be used for breast ductal cancer diagnosis. Here, we identified miR-1273g-3p as a biomarker for detecting breast ductal cancer. We detected miR-1273g-3p levels in the plasma of 39 sporadic breast ductal cancer patients and 40 healthy donors by Stem-loop Quantitative Real-time PCR (qRT-PCR). The results showed the plasma miR-1273g-3p level were significantly up-regulated in breast ductal cancer patients compared with healthy donors (p=0.0139). Receiver operating characteristic (ROC) curve also revealed the significantly diagnostic ability of miR-1273g-3p in patients (p=0.0414). In addition, the plasma level of miR-1273g-3p was closely related to IIIB-IIIC TNM stage. We also confirmed the higher expression level of miR-1273g-3p in breast cancer cell lines MCF-7 (4.872±0.537) than normal breast cells (Hs 578Bst). Taken together, miR-1273g-3p could represent as a potential biomarker for early breast ductal cancer diagnosis.

Resumo Introdução: Ainda não há um método universal estabelecido para diferenciar entre a leucoplasia benigna e maligna ou identificar as leucoplasias das pregas vocais. Objetivo: Avaliar a confiabilidade de uma classificação morfológica e a correlação entre os tipos morfológicos e os graus histopatológicos das leucoplasias de pregas vocais. Método: Os registros de 375 pacientes com leucoplasia da prega vocal assistidos entre 2009 e 2015 foram revisados retrospectivamente. Dois observadores dividiram a leucoplasia da prega vocal entre tipo plano e liso, elevado e liso, e rugoso, com base na aparência morfológica. A confiabilidade interobservador foi avaliada e os resultados de classificação de ambos os observadores foram comparados com os graus histopatológicos finais. As características clínicas entre os grupos de baixo risco e alto risco também foram analisadas. Resultados: A porcentagem da concordância interobservador da classificação morfológica foi de 78,7% (κ = 0,615, p < 0,001). Nos resultados de ambos os observadores, os tipos morfológicos correlacionaram-se significativamente com os graus histopatológicos (p1 < 0,001, p2 < 0,001, teste de Kruskal-Wallis; r1 = 0,646, p1 < 0,001, r2 = 0,539, p2 < 0,001, análise de correlação de Spearman). A análise multivariada mostrou que a idade do paciente (p = 0,018), o tamanho da lesão (p < 0,001) e o tipo morfológico (p < 0,001) foram significativamente diferentes entre o grupo de baixo risco e o de alto risco. A análise da curva ROC (Receiver Operating Characteristic) combinada de parâmetros significativos revelou uma área sob a curva de 0,863 (IC 95%: 0,823 ± 0,903, p < 0,001). Conclusões: A classificação morfológica proposta para leucoplasia de prega vocal foi consistente entre observadores e os tipos morfológicos correlacionaram-se com os graus histopatológicos. A idade do paciente, o tamanho da lesão e o tipo morfológico podem permitir a estratificação de risco e fornecem diretrizes de tratamento para a leucoplasia da prega vocal.

Abstract Introduction: There is still no general method for discriminating between benign and malignant leukoplakia and identifying vocal fold leukoplakia. Objective: To evaluate the reliability of a morphological classification and the correlation between morphological types and pathological grades of vocal fold leukoplakia. Methods: A total of 375 patients with vocal fold leukoplakia between 2009 and 2015 were retrospectively reviewed. Two observers divided the vocal fold leukoplakia into flat and smooth, elevated and smooth, and rough type on the basis of morphological appearance. The inter-observer reliability was evaluated and the results of classification from both observers were compared with final pathological grades. Clinical characteristics between low risk and high risk group were also analyzed. Results: The percentage inter-observer agreement of the morphological classification was 78.7% (κ = 0.615, p < 0.001). In the results from both observers, the morphological types were significantly correlated with the pathological grades (p1 < 0.001, p2 < 0.001, Kruskal-Wallis test; r1 = 0.646, p1 < 0.001, r2 = 0.539, p2 < 0.001, Spearman Correlation Analysis). Multivariate analysis showed patient's age (p = 0.018), the size of lesion (p < 0.001), and morphological type (p < 0.001) were significantly different between low risk group and high risk group. Combined receiver operating characteristic curve analysis of significant parameters revealed an area under the receiver operating characteristic curve of 0.863 (95% CI 0.823-0.903, p < 0.001). Conclusions: The proposed morphological classification of vocal fold leukoplakia was consistent between observers and morphological types correlated with pathological grades. Patient's age, the size of lesion, and morphological type might enable risk stratification and provide treatment guidelines for vocal fold leukoplakia.

RESUMO: As influências do emprego de subprodutos que contêm silício, cálcio, magnésio e potássio, sobre a qualidade do solo ácido na Península de Jiaodong da China, foram estudadas e comparadas com a da cal através do monitoramento das propriedades físico-químicas e atividades enzimáticas do solo ácido ao longo de um 120- período do dia. Os resultados mostraram que as alterações dos subprodutos e a cal aumentaram significativamente as atividades de pH, CE e enzimas do solo. As alterações dos subprodutos obviamente inibiram o carbono da biomassa microbiana e a respiração do solo, mas o solo tratado com cal apresentou um maior nível de emissão de CO2. Em comparação com as modificações do subproduto, o solo tratado tinha o maior pH, a atividade da peroxidase, a atividade da fenol oxidase e a atividade da invertease. Portanto, o cal pode ser uma escolha melhor em relação às alterações dos subprodutos para melhorar as propriedades químicas e biológicas do solo ácido na Península de Jiaodong da China.

ABSTRACT: The influences of byproduct amendments, containing silicon, calcium, magnesium and potassium, on acidic soil quality in Jiaodong Peninsula of China had been studied and compared with that of lime through monitoring physicochemical properties and enzyme activities of acidic soil over a 120-day period. Byproduct amendments (1125, 2250, 4500 and 9000 kg ha-1) and lime 2250 kg ha-1 was applied in the acidic soil. Results showed that both byproduct amendments and lime significantly increased the pH, EC and enzyme activities of soil. The by-product amendments inhibited microbial biomass carbon and soil respiration. Nevertheless, the lime-treated soil had a much more higher level of CO2 emission than the by-product amendments-treated soil. Compared to the by-product amendments-treated soil, the lime-treated soil had the higher pH, peroxidase activity, phenol oxidase activity and invertase activity. Therefore, lime might be a better choice over by-product amendments to improve chemical and biological properties of the acidic soil in Jiaodong Peninsula of China. For soils lacking available calcium and magnesium, the mixture of 4500 kg ha-1 amendments and 2250 kg ha-1 lime was recommend to treat the soil.

Abstract Purpose: To investigate the influence of dexmedetomidine on myocardial ischemia-reperfusion injury (IRI) in rabbits. Methods: Twenty-four New Zealand white rabbits were randomly divided into two equal-sized groups: IRI group (group IR) and dexmedetomidine group (group D). Systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR), left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), left ventricular diastolic pressure (LVDP), +dp/dtmax, -dp/dtmax, and t-dp/dtmax were recorded and calculated at the following time points: before (T0) and after (T1) dexmedetomidine infusion, after 30-min ischemia (T2), and after 120-min reperfusion (T3). The levels of plasma endothelin 1 (ET-1), thromboxane A2 (TXA2), and platelet activating factor (PAF); area of myocardial infarction (MI); and no-reflow area were evaluated. Results: SBP, DBP, LVSP, LVEDP, LVDP, and +dp/dtmax at T3 were higher in group D than in group IR (P<0.05). The average no-reflow area in group IR was significantly smaller than that in group D (14±3% vs. 38±5%, P=0.0116). The ET-1, TXA2, and PAF levels at T2 and T3 were higher than those at T0 in both groups (P<0.05). Conclusion: Dexmedetomidine could reduce the magnitude of ischemic myocardial no-reflow area and protect the myocardium with ischemia-reperfusion injury.

摘要目的 旨在评估初级医疗质量在中国实行卫生系统改革的两个特大城市中发生的变化。方法 我们针对 2011 年和 2013 年在上海以及 2012 年和 2013 年在深圳就诊于社区卫生中心的患者开展了一项多阶段、分层、随机式的面对面调查。通过评估工具对初级医疗的质量进行了衡量。基于多元线性回归的双重差分法分析用于对比在控制了潜在的混杂因素后,一段时间内发生的变化。结果 3214 名参与者中的大多数人 (2721) 都将社区卫生中心作为自己常规就诊的医疗机构,这些都包括在我们的分析中。上海和深圳的初级医疗质量的总均分在基准值上很相似。在深圳,所有参与者和低收入人群的总均分已分别降低了 0.922(95% CI:0.629 至 1.215)和 1.203(95% CI:0.397 至 2.009)。然而在上海,总均分却有所提高,这种情况包括在第一线就诊渠道利用率、持续性、信息协调性和全面性方面的分值提高。结论上海的初级医疗质量有所提高,而深圳并非如此。这可能是因为,在上海实行按人头付费并依据当地卫生工作重点提供服务,促使患者与全科医生之间保持长期有益的关系。

RésuméObjectif Évaluer les changements dans la qualité des soins de santé primaires, dans deux mégapoles chinoises, après l'introduction de réformes dans le système de santé en Chine.Méthodes Nous avons réalisé des enquêtes en face à face aléatoires stratifiées à plusieurs degrés auprès des patients des centres de santé communautaires de Shanghai en 2011 et 2013 et de Shenzhen en 2012 et 2013. La qualité des soins de santé primaires a été mesurée à l'aide d'un outil d'évaluation. Des analyses de l'écart des différences basées sur des régressions linéaires multiples ont été réalisées afin de comparer les changements survenus au fil du temps, après contrôle des facteurs de confusion potentiels.Résultats Sur les 3 214 participants, la majorité (2 721 personnes) a indiqué fréquenter régulièrement le centre de santé communautaire et a été incluse dans notre analyse. Au départ, les scores totaux moyens attribués à la qualité des soins primaires ont été similaires à Shanghai et à Shenzhen. À Shenzhen, les scores totaux moyens attribués par tous les participants et par les participants à faible revenu ont respectivement baissé de 0,922 (IC 95 % : de 0,629 à 1,215) et de 1,203 (IC 95% : de 0,397 à 2,009). À Shanghai, en revanche, les scores totaux moyens ont progressé, notamment avec l'amélioration des scores associés aux critères " utilisation de premier recours ", " continuité ", " coordination de l'information " et " exhaustivité ".Conclusion La qualité des soins primaires s'est améliorée à Shanghai mais pas à Shenzhen. Cela est peut-être lié au fait qu'à Shanghai, les relations bénéfiques de longue date instaurées entre les patients et les généralistes ont été soutenues par des paiements par capitation et par la fourniture de services adaptés aux priorités de santé locales.

абстрактный доступен в Полный текст

AbstractObjective To assess changes in the quality of primary care in two megacities following the introduction of health system reforms in China.Methods We conducted multistage stratified random face-to-face surveys of patients visiting community health centres in Shanghai in 2011 and 2013, and Shenzhen in 2012 and 2013. Quality of primary care was measured using an assessment tool. Difference-in-difference analyses based on multiple linear regressions were used to compare the changes over time, after controlling for potential confounders.Findings Most (2721) of the 3214 participants used a community health centre as their regular source of care and were included in our analyses. The mean total scores for quality of primary care were similar for Shanghai and Shenzhen at baseline. In Shenzhen, the mean total scores for all participants and those on low incomes had worsened by 0.922 (95% CI: 0.629 to 1.215) and 1.203 (95% CI: 0.397 to 2.009), respectively. In Shanghai, however, there were improvements in the mean total scores which included increases in the scores for first-contact utilization, continuity, coordination of information and comprehensiveness.Conclusion The quality of primary care improved in Shanghai but not in Shenzhen. This may be because, in Shanghai, beneficial long-term relationships between patients and general practitioners were supported by capitation payments and the provision of services tailored to the local health priorities.

ملخصالغرض تقييم التغيرات في جودة الرعاية الصحية الأولية في اثنتين من المدن الكبرى في أعقاب إدخال إصلاحات على النظام الصحي في الصين.الطريقة أجرينا مسوحات عشوائية متطبِّقة متعددة المراحل وجهًا لوجه للمرضى الذين يترددون على المراكز الصحية المجتمعية في شانغهاي في عاميّ 2011 و2013، وشنجن في عاميّ 2012 و2013. وقد تم قياس جودة الرعاية الصحية الأولية باستخدام أداة للتقييم. تم استخدام أسلوب الاختلافات في الفارق المستند إلى التحوفات الخطية المتعددة لمقارنة التغيرات بمرور الوقت، بعد ضبط المتغيرات المحيّرة المحتملة.النتائج استخدم معظم المشاركين البالغ عددهم (2721) من أصل 3214 مشاركًا المراكز الصحية المجتمعية كمصدر منتظم للرعاية وتم تضمينهم في التحليل الذي أجريناه. وكانت الأحراز الكلية الوسطية لجودة الرعاية الصحية الأولية مشابهة لشانغهاي وشنجن عند خط الأساس. في شنجن، ساءت متوسط الأحراز الكلية لجميع المشاركين والأشخاص ذوي الدخل المنخفض بنسبة تبلغ 0.922 (بنسبة أرجحية مقدارها 95%: 0.629 إلى 1.215) و1.203 (بنسبة أرجحية مقدارها 95%: 0.397 إلى 2.009)، على التوالي. أما في شانغهاي، فقد كانت هناك تحسينات في متوسط الأحراز الكلية والتي تضمنت زيادات في أحراز الانتفاع للاتصال الأول، والاستمرارية، وتنسيق المعلومات وشموليتها.الاستنتاج لقد تحسنت جودة الرعاية الصحية الأولية في شانغهاي لكنها لم تتحسن في شنجن. ويمكن تفسير ذلك بأنه في شنغهاي، تم دعم علاقات مفيدة طويلة الأمد بين المرضى والممارسين العموميين من خلال دفع الأجرة على الفرد وتوفير خدمات توضع تبعًا لأولويات الصحة المحلية.

ResumenObjetivo Evaluar los cambios en la calidad de la atención primaria en dos megaciudades tras la introducción de las reformas del sistema sanitario en China.Métodos Se llevaron a cabo encuestas cara a cara aleatorias y estratificadas en varias etapas a pacientes que visitaron los centros sanitarios comunitarios de Shanghai en 2011 y 2013 y de Shenzhen en 2012 y 2013. La calidad de la atención primaria se midió utilizando una herramienta de evaluación. Los análisis de diferencias en diferencias basados en regresiones lineales múltiples se utilizaron para comparar los cambios a lo largo del tiempo tras haber controlado los posibles factores de confusión.Resultados La mayoría (2.721) de los 3.214 participantes utilizaban un centro sanitario comunitario como su fuente regular de atención primaria y fueron incluidos en nuestros análisis. Las puntuaciones medias totales de la calidad de la atención primaria eran similares para Shanghai y Shenzhen al inicio del estudio. En Shenzhen, las puntuaciones medias totales de todos los participantes y de aquellos con bajos ingresos había empeorado en un 0,922 (IC del 95%: de 0,629 a 1,215) y un 1,203 (IC del 95: de 0,397 a 2,009), respectivamente. Sin embargo, en Shanghai hubo mejoras en las puntuaciones medias totales, incluidos incrementos en las puntuaciones de la utilización de primer contacto, la continuidad, la coordinación de la información y la exhaustividad.Conclusión La calidad de la atención primaria mejoró en Shanghai pero no en Shenzhen. Esto se puede deber a que en Shanghai, las relaciones beneficiosas a largo plazo entre los pacientes y los médicos generales estaban respaldadas por pagos de capitación y la prestación de servicios adaptados a las prioridades sanitarias locales.

The Toll-like receptor 5 (TLR5) recognizes flagellin of Gram-positive and -negative bacteria and plays an important role in the host defense system. Here, we surveyed single nucleotide polymorphisms (SNPs) in the coding sequence of the porcine TLR5 gene in 83 individuals from five pig breeds, these including Chinese local populations and Western commercial pig breeds. A total of 19 medium polymorphic SNPs (0.25 < PIC < 0.5) were identified, three of which were missense mutations that clustered within the extracellular domain of TLR5. One of the non-synonymous SNPs fell within a 228-amino acid region which has been shown to be important for flagellin recognition. Four SNPs were only found with high frequencies in Oriental pig breeds. The 19 SNPs were found in 30 haplotypes, one of which segregated at high frequency in all samples. Compared with Western pig breeds, Chinese local populations had higher genetic diversity and more haplotypes. Tajima's test showed no evidence for deviation from neutrality. The data provide useful information for future genetic marker characterization by means of disease association analysis and/or stimulating the mutation carrier with relevant ligands.

This paper reports on the development and validation of a loop-mediated isothermal amplification assay (LAMP) for the rapid and specific detection of Actinobacillus pleuropneumoniae (A. pleuropneumoniae). A set of six primers were designed derived from the dsbE-like gene of A.pleuropneumoniae and validate the assay using 9 A. pleuropneumoniae reference/field strains, 132 clinical isolates and 9 other pathogens. The results indicated that positive reactions were confirmed for all A. pleuropneumoniae strains and specimens by LAMP at 63ºC for 60 min and no cross-reactivity were observed from other non-A.pleuropneumoniae including Haemophilus parasuis, Escherichia coli, Pasteurella multocida, Bordetella bronchiseptica, Streptococcus suis, Salmonella enterica, Staphylococcus, porcine reproductive and respiratory syndrome virus (PRRSV), and Pseudorabies virus. The detection limit of the conventional PCR was 10² CFU per PCR test tube, while that of the LAMP was 5 copies per tube. Therefore, the sensitivity of LAMP was higher than that of PCR. Moreover, the LAMP assay provided a rapid yet simple test of A. pleuropneumoniae suitable for laboratory diagnosis and pen-side detection due to ease of operation and the requirement of only a regular water bath or heat block for the reaction.

A investigação de extrato acetônico de folhas e caules de Daphne feddei forneceu três novos fenilpropanóides, fedeicetonas A-C (1-3), juntamente com nove fenilpropanóides conhecidos (4‑11). Suas estruturas foram elucidadas com base em métodos espectroscópicos. Os compostos 1-3 foram testados com relação as suas atividades anti-HIV-1 e citotoxidades frente a células cancerígenas. Os resultados mostraram que as atividades citotóxicas e anti-HIV-1 dos compostos 1-3 são modestas.

Investigation of acetone extract of the leaves and stems of Daphne feddei afforded three new phenylpropanoids, feddeiketones A-C (1-3), together with nine known phenylpropanoids (4-11). Their chemical structures were elucidated on the basis of spectroscopic methods. Compounds 1-3 were tested for their anti-HIV-1 activities and cytotoxicities against cancer cells. The results showed that compounds 1-3 have modest cytotoxic and anti-HIV-1 activities.