A series of 4H-pyran derivatives were synthesized through a three-component condensation reaction in a pot using aromatic aldehyde, cyclohexanedione, and malononitrile in the dodecyl benzenesulfonic acid/H2O microemulsion system. This protocol is attractive because dodecyl benzenesulfonic acid microemulsion is superior for organic reaction due to its dual catalysis. On the one hand, dodecyl benzenesulfonic acid acts as a Brønsted acid to catalyze the reaction; on the other hand, it can form a microemulsion as surfactant to enlarge the boundary area and entrap water produced during esterification reaction. In addition, the reaction process is simple, with high liaison formation effciency, good yields, and environmentally harmless reaction conditions. The dodecyl benzenesulfonic acid/H2O microemulsion system was equally effective when used in consecutive reactions. 4Hpyran Hpyran 4H pyran H threecomponent three component aldehyde cyclohexanedione acidH2O acidHO H2O O catalysis hand addition simple effciency yields conditions reactions acidH HO

ABSTRACT: This study investigated the differences in gene expression profiles of diploid and autotetraploid in cowpea, and provided theoretical basis for screening key genes of differential expression and ploidy breeding. The phenotypes and contents of chlorophyll, soluble sugar and soluble protein of diploid and autotetraploid of cowpea were compared and transcriptome sequencing was performed. The autotetraploid leaves of cowpea were thicker and darker green than diploid leaves, and the contents of chlorophyll, soluble sugar and soluble protein in leaves were higher. A total of 2678 differentially expressed genes (DEGs) were analyzed in the diploid and autotetraploid of cowpea. Among them, there were 421 genes with higher expression of tetraploid than diploid, and 2257 genes with lower expression of tetraploid than diploid. All 2678 DEGs were annotated into the Gene Ontology (GO) functional library. The DEGs were mainly concentrated in metabolism and cell composition. Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway analysis showed that cowpea diploid and autotetraploid have significant differences in flavonoid biosynthesis, degradation of other glycan, phenylpropane biosynthesis, starch sucrose metabolism, keratin, amber and wax biosynthesis, circadian rhythm and plant pathways. ABSTRACT breeding chlorophyll performed 267 (DEGs them 42 225 GO (GO library composition KEGG (KEGG biosynthesis glycan keratin pathways 26 4 22 2

RESUMO: O objetivo deste estudo foi investigar as diferenças nos perfis de expressão gênica de diplóides e autotetraplóides em feijão-caupi e fornecer base teórica para a triagem de genes-chave de expressão diferencial e melhoramento de ploidia. Os fenótipos e teores de clorofila, açúcar solúvel e proteína solúvel de diplóides e autotetraplóides de feijão-caupi foram comparados e o sequenciamento do transcriptoma foi realizado. As folhas autotetraploides do feijão-caupi apresentaram-se mais espessas e de cor verde mais escura que as folhas diplóides, e os teores de clorofila, açúcar solúvel e proteína solúvel nas folhas foram maiores. Um total de 2678 genes diferencialmente expressos (DEG) foram analisados no diplóide e autotetraploide do feijão-caupi. Entre eles, havia 421 genes com maior expressão de tetraploide do que diplóides, e 2257 genes com menor expressão de tetraploide do que diplóides. Todos os 2678 DEGs foram anotados na biblioteca funcional GO. Os DEGs foram concentrados principalmente no metabolismo e na composição celular. A análise da via KEGG mostrou que o diplóides e o autotetraplóide do feijão-caupi apresentam diferenças significativas na biossíntese de flavonoides, degradação de outros glicanos, biossíntese de fenilpropano, metabolismo da sacarose do amido, biossíntese de queratina, âmbar e cera, ritmo circadiano e vias vegetais. RESUMO feijãocaupi feijão caupi geneschave chave ploidia clorofila realizado apresentaramse apresentaram se maiores 267 DEG (DEG feijãocaupi. caupi. eles 42 225 GO celular flavonoides glicanos fenilpropano amido queratina cera vegetais 26 4 22 2

ABSTRACT Against the background of a sharp decline in soybean planting, rising imports, and natural disasters in China, finding appropriate distribution areas for soybean has become a matter of urgency so that soybean planting policies can be formulated and soybean food security ensured. Among the numerous ecological niche models, the most suitable one for predicting potential distribution areas of soybean in the frigid region must be identified. Based on 65 soybean occurrence points and nine environmental variables, three ecological niche models, MaxEnt, BIOCLIM, and DOMAIN, were applied to the prediction of potential distribution areas for soybean. According to the analytical comparison, the three models predicted the potential distribution of soybean and, specifically, MaxEnt, stood out above the other two models as regards predicting the soybean distribution (Receiver Operating Characteristic curve, AUC = 0.916, Kappa = 0.685). The potential distribution areas (from low suitability to high suitability) predicted by MaxEnt was the largest and accounted for 59.5 % of the total area. The potential suitable distribution area of soybean was mainly concentrated in relatively flat terrain. The Sanjiang Plain and the Northeast Plain accounted for 9.4 % of the total area in the frigid region and are highly suitability for soybean. At the same time, annual mean temperature, elevation and Apr solar radiation were the key determinants affecting soybeans’ habitat. On the whole, the selection of ecological niche models and the prediction of soybean potential distribution can provide an essential reference for soybean planting and planning. Moreover, it would be a reliable example for the subsequent related research on soybean habitats. imports China ensured identified 6 variables BIOCLIM DOMAIN comparison specifically Receiver curve 0916 0 916 0.916 0.685. 0685 0.685 . 685 0.685) from 595 59 5 59. terrain 94 9 4 9. time temperature soybeans habitat whole planning Moreover habitats 091 91 0.91 068 0.68 68 09 0.9 06 0.6 0.

Abstract To investigate the role of Peg13 in modulating the inflammatory response in sepsis, we established Lipopolysaccharide (LPS)-induced 293T cells and mouse models. Peg13 expression was assessed at various time points after infection using RT-qPCR. The levels of high mobility group box 1 (HMGB1) and interleukin-6 (IL-6) were quantified through ELISA. A total of 44 septic patients and 36 healthy participants were recruited to measure Peg13 and HMGB1 levels in the blood. Peg13 demonstrated significant down-regulation in the supernatant of LPS-induced 293T cells and in the blood of LPS-induced mice. Moreover, the levels of proinflammatory cytokines HMGB1 and IL-6 were elevated in both the supernatant of LPS-induced cell models and blood specimens from LPS-induced murine models, and this elevation could be notably reduced by Peg13 suppression. In a clinical context, Peg13 and HMGB1 levels were higher in septic patients compared to healthy subjects. Peg13 exhibited a negative correlation with HMGB1, C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR) among septic patients. Peg13 mitigates the inflammatory response by reducing the release of proinflammatory cytokines HMGB1 and IL-6 in sepsis, presenting a potential therapeutic target for alleviating inflammation in sepsis treatment. Peg Peg1 LPSinduced LPS induced T RTqPCR. RTqPCR RT qPCR. qPCR RT-qPCR HMGB (HMGB1 interleukin6 interleukin 6 interleukin- IL6 IL (IL-6 ELISA 4 3 downregulation down regulation mice Moreover IL- suppression context subjects Creactive C reactive CRP, CRP , (CRP) ESR (ESR treatment (HMGB (IL- (CRP (IL

RESUMO: Neste estudo, compararmos a fenologia da floração, os açúcares solúveis e as proteínas solúveis do pólen, as enzimas antioxidantes e as características de armazenamento de cinco populações cultivadas de Paeonia ostii ‘Feng dan’ em diferentes altitudes em Luoyang, na perspectiva da biologia da polinização. O pólen é um fruto de uma espécie de árvore, com um teor de proteínas solúveis e de pólen, sistema de enzimas antioxidantes e características de armazenamento. Os resultados mostraram que o início da floração foi mais tardio em altitudes mais elevadas. O teor de açúcar solúvel do pólen variou significativamente entre diferentes locais, não havendo uma relação óbvia com a altitude, enquanto o teor de proteínas solúveis tendeu a diminuir com o aumento da altitude. As atividades das enzimas antioxidantes do pólen de populações cultivadas a diferentes altitudes diferiram entre si, as atividades das enzimas SOD e POD aumentaram basicamente com a diminuição da temperatura, enquanto a enzima CAT apresentou várias tendências. Verificaram-se diferenças no tempo de armazenamento do pólen de populações de diferentes altitudes, e o pólen de populações de altitudes mais elevadas parecia estar mais bem adaptado a ambientes de armazenamento a baixas temperaturas. A taxa de redução da viabilidade do pólen sob armazenamento seco a -74,7 °C foi significativamente menor do que sob outras condições de armazenamento. As análises sugerem que as diferenças entre as populações de cultivo de ‘Fengdan’ na mesma região a diferentes altitudes podem ser exploradas para efetuar a polinização cruzada entre populações próximas, utilizando a tecnologia de armazenamento de pólen por secagem a baixa temperatura, com vista a atingir o objetivo de produção abundante e em grande escala de peónia oleaginosa. RESUMO estudo Feng dan Luoyang árvore locais altitude si temperatura tendências Verificaramse Verificaram se temperaturas 74,7 747 74 7 -74, C ‘Fengdan Fengdan próximas oleaginosa 74, -74 -7 -

ABSTRACT: From a pollination biology standpoint, this study compared modifications in the flowering phenology, soluble sugar and protein content of pollen, antioxidant enzyme system, and storage traits of five cultivated populations of Paeonia ostii ‘Feng dan’ at various altitudes in Luoyang. The findings indicated that the first blooming phase was delayed at greater altitudes. There was a significant difference in the soluble sugar content of pollen between sites; and there was no discernible relationship between soluble sugar content and altitude. With increasing altitude, there is a tendency for soluble protein content to decrease. The activities of antioxidant enzymes in pollen from cultivated populations at varying altitudes showed SOD and POD enzyme activities generally increasing with decreasing temperature, whereas CAT enzymes showed divergent patterns. Differences existed in the storage time of pollen across populations at different altitudes, and pollen from higher altitudes appeared to be better adapted to low-temperature storage environments. The rate of decrease in pollen viability under dry storage at -74.7 °C was significantly lower than under other storage conditions. The comprehensive analysis concludes that the variations in ‘Feng dan’ cultivation populations within the same region at different altitude zones can be leveraged for cross-pollination among proximate populations, utilizing low-temperature dry pollen storage technology, with the aim of achieving abundant oil peony production. ABSTRACT standpoint phenology system Feng dan Luoyang sites temperature patterns lowtemperature low environments 74.7 747 74 7 -74. C conditions crosspollination cross technology production 74. -74 -7 -

Emerging evidence demonstrates that curcumin has an inhibitory effect on non-small cell lung cancer (NSCLC), and its targets and mechanism of action need further exploration. The goal of this study was to explore the potential targets and mechanism of curcumin against NSCLC by network pharmacology, bioinformatics, and experimental validation, thereby providing more insight into combination treatment with curcumin for NSCLC in preclinical and clinical research. Curcumin targets against NSCLC were predicted based on HIT2.0, STD, CTD, and DisGeNET, and the core targets were analyzed via protein-protein interaction network construction (PPI), Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and molecular docking. The gene expression levels of samples in A549 cells, NCI-H460, and curcumin treated groups were detected by real-time quantitative PCR. A total of 67 common targets between curcumin and NSCLC were collected by screening public databases. GO and KEGG analysis suggested that curcumin treatment of NSCLC mainly involves cancer-related pathways, such as PI3K-AKT signaling pathway, Foxo signaling pathway, microRNAs, MAPK signaling pathway, HIF-1 signaling pathway, etc. The targets with the highest degree were identified through the PPI network, namely CASP3, CTNNB1, JUN, IL6, MAPK3, HIF1A, STAT3, AKT1, TP53, CCND1, VEGFA, and EGFR. The results of the in vitro experiments showed that curcumin treatment of NSCLC down-regulated the gene expressions of CCND1, CASP3, HIF1A, IL-6, MAPK3, STAT3, AKT1, and TP53. Our findings revealed that curcumin functions as a potential therapeutic candidate for NSCLC by suppressing multiple signaling pathways and interacting with multiple gene targets. nonsmall non small NSCLC, , (NSCLC) exploration pharmacology bioinformatics validation research HIT20 HIT HIT2 0 HIT2.0 STD CTD DisGeNET proteinprotein protein PPI, (PPI) GO, (GO) KEGG, (KEGG) docking A54 cells NCIH460, NCIH460 NCIH NCI H460, H460 H NCI-H460 realtime real time PCR 6 databases cancerrelated related PI3KAKT PIKAKT PI3K AKT PI K pathway microRNAs HIF1 HIF 1 HIF- etc CASP3 CASP CTNNB1 CTNNB JUN IL6 IL MAPK3 HIF1A HIFA STAT3 STAT AKT1 TP53 TP CCND1 CCND VEGFA EGFR downregulated down regulated 6, IL-6 (NSCLC HIT2. (PPI (GO (KEGG A5 NCIH46 H46 NCI-H46 KAKT PIK TP5 IL- NCIH4 H4 NCI-H4 NCI-H

ABSTRACT Purpose: To compare the effectiveness and safety of marketed oral drugs for overactive bladder based on a systematic review and network meta-analysis approach. Methods: Pubmed, Embase, Web of Science, and the Cochrane Register of Clinical Trials databases were systematically searched. The search time frame was from database creation to June 2, 2022. Randomized controlled double-blind trials of oral medication for overactive bladder were screened against the protocol's entry criteria. Trials were evaluated for quality using the Cochrane Risk of Bias Assessment Tool, and data were statistically analyzed using Stata 16.0 software. Result: A total of 60 randomized controlled double-blind clinical trials were included involving 50,333 subjects. Solifenacin 10mg was the most effective in mean daily micturitions and incontinence episodes, solifenacin 5/10mg in mean daily urinary urgency episodes and nocturia episodes, fesoterodine 8mg in urgency incontinence episodes/d and oxybutynin 5mg in voided volume/micturition. In terms of safety, solifenacin 5mg, ER-tolterodine 4mg, mirabegron, vibegron and ER-oxybutynin 10mg all showed a better incidence of dry mouth, fesoterodine 4mg, ER-oxybutynin 10mg, tolterodine 2mg, and vibegron in the incidence of constipation. Compared to placebo, imidafenacin 0.1mg showed a significantly increased incidence in hypertension, solifenacin 10mg in urinary tract infection, fesoterodine 4/8mg and darifenacin 15mg in headache. Conclusion: Solifenacin showed better efficacy. For safety, most anticholinergic drugs were more likely to cause dry mouth and constipation, lower doses were better tolerated. The choice of drugs should be tailored to the patient's specific situation to find the best balance between efficacy and safety. Purpose metaanalysis meta analysis approach Methods Pubmed Embase Science searched 2 2022 doubleblind double blind protocols protocol s criteria Tool 160 16 0 16. software Result 6 50333 50 333 50,33 subjects mg 510mg 5 episodesd d volumemicturition volume micturition volume/micturition ERtolterodine ER 4mg mirabegron ERoxybutynin 2mg constipation placebo 01mg 1mg hypertension infection 48mg 4 headache Conclusion tolerated patients patient 202 1 5033 33 50,3 20 503 3 50,

ABSTRACT Top-quality pellets can significantly increase density and durability of agricultural residues, reducing logistic costs. However, these pellets depend on numerous parameters, including feedstock properties and production conditions. To ensure high-quality pellets, a single-factor experiment and the response surface methodology were used to investigate the effects of particle size, moisture content, molding pressure, pelleting time, ultrasonic power, and interaction effects between variables on density and durability of pellets for ultrasonic vibration-assisted pelleting of corn stover. The response surface models between variables and response were established. The results showed that all variables affect the density and durability of pellets. An optimal condition for density and durability was obtained, and a further experiment was conducted to validate the values. The results suggested that desirability (0.999) under optimal conditions confirmed the validation of models. The optimal combination of process parameters included particle size of 1.5 mm, moisture content of 10 %, molding pressure of 379 kPa, pelleting time of 80 s, ultrasonic power of 250 W, with values of 1,381.14 kg m–3 and 97.58 % for density and durability of pellets, respectively. Topquality Top quality residues costs However highquality high singlefactor single factor vibrationassisted vibration assisted stover established obtained 0.999 0999 0 999 (0.999 15 1 5 1. mm 37 kPa 8 s 25 W 138114 381 14 1,381.1 m3 m 3 m– 9758 97 58 97.5 respectively 0.99 099 99 (0.99 2 13811 38 1,381. 975 9 97. 0.9 09 (0.9 1381 1,381 0. (0. 138 1,38 (0 13 1,3 ( 1,

Abstract Objective At present, bleomycin has been widely used in the treatment of Lymphatic Malformations (LMs). This study aims to perform a meta-analysis to investigate the effectiveness and influencing factors of bleomycin in the treatment of LMs. Methods We conducted a systematic review and meta-analysis to clarify the relationship between bleomycin and LMs. PubMed, ISI Web of Science and MEDLINE were searched. Results A total of 21 studies (including 428 cases) about bleomycin sclerotherapy for LMs were included in the current meta-analyses. We calculated pooled effective rate and 95% Confidence Interval (95% CI) using random effects model to evaluate the relations between bleomycin and LMs. The results suggested that the effective rate of bleomycin was the combined effective rate was 84.0% (95% CI 0.81‒0.87) and ranged from 39% (95% CI 0.22‒0.56) to 94% (95% CI 0.87-1.02). The heterogeneity among the studies was substantial (I2 = 61.7%, p = 0.000). In subgroup analyses, it was observed that among retrospective study and prospective study, the estimated effective rate was 80.0% (95% CI 0.76‒0.84) and 91.0% (95% CI 0.85‒0.97), respectively. In terms of the dosage, the combined effective rates of weight-based group and fixed-dose group were 86% (95% CI 0.83‒0.90) and 74.0% (95% CI 0.66‒0.82), respectively. There was no significant publication bias in Egger's test (p = 0.059, 95% CI −3.81 to 0.082), but Begg's test did (p = 0.023), and the funnel plot is asymmetric. Conclusion Our study suggested that bleomycin was safe and effective in the treatment of LMs and was primarily dose dependent. present . (LMs) metaanalysis meta analysis PubMed searched 2 including 42 cases metaanalyses. metaanalyses analyses. analyses meta-analyses 95 (95 840 84 0 84.0 0.81‒0.87 081087 81 87 39 0.22‒0.56 022056 22 56 94 0.871.02. 087102 0.87 1.02 1 02 0.87-1.02) I2 I (I 617 61 7 61.7% 0.000. 0000 0.000 000 0.000) 800 80 80.0 0.76‒0.84 076084 76 910 91 91.0 0.85‒0.97, 085097 0.85‒0.97 , 85 97 0.85‒0.97) respectively dosage weightbased weight based fixeddose fixed 86 0.83‒0.90 083090 83 90 740 74 74.0 0.66‒0.82, 066082 0.66‒0.82 66 82 0.66‒0.82) Eggers Egger s 0059 059 0.059 381 3 −3.8 0.082, 0082 0.082 082 0.082) Beggs Begg 0.023, 0023 0.023 023 0.023) asymmetric dependent (LMs 4 9 (9 8 84. 0.81‒0.8 08108 0.22‒0.5 02205 5 871 0.871.02 08710 087 0.8 102 1.0 0.87-1.02 6 61.7 0.00 00 80. 0.76‒0.8 07608 91. 08509 0.85‒0.9 0.83‒0.9 08309 74. 06608 0.66‒0.8 005 05 0.05 38 −3. 008 0.08 08 002 0.02 ( 0.81‒0. 0810 0.22‒0. 0220 0.871.0 0871 0. 10 1. 0.87-1.0 61. 0.0 0.76‒0. 0760 0850 0.85‒0. 0.83‒0. 0830 0660 0.66‒0. −3 0.81‒0 081 0.22‒0 022 0.871. 0.87-1. 0.76‒0 076 085 0.85‒0 0.83‒0 083 066 0.66‒0 − 0.81‒ 0.22‒ 0.871 0.87-1 0.76‒ 07 0.85‒ 0.83‒ 06 0.66‒ 0.81 0.22 0.87- 0.76 0.85 0.83 0.66 0.2 0.7 0.6

ABSTRACT Due to their tiny size, soil nematodes and plant-parasitic nematodes are challenging to homogenize and collect using traditional mortar and pestle methods. To overcome this, we developed a reliable and efficient nematode preparation and grinding method using the BioPulverizer. The method involves creating a modified sample ‘sandwich’ with liquid nitrogen-frozen nematode samples placed between two layers of aluminum foil. This ‘sandwich’ facilitates complete homogenization through cryogenic grinding without any sample loss during transfer. The powerful nematode grinding yields high-quality and high-quantity samples, suitable for nucleic acid release and subsequent molecular identification and other downstream applications. size plantparasitic plant parasitic methods this BioPulverizer ‘sandwich sandwich nitrogenfrozen nitrogen frozen foil transfer highquality high quality highquantity quantity applications

RESUMEN Introducción: El entrenamiento debe realizarse de acuerdo con las características del baloncesto estableciendo un sistema de índices de evaluación científica para explotar plenamente el potencial de sus jugadores. Objetivo: Evaluar el sistema de índices de medición del entrenamiento físico específico para jugadores de baloncesto. Métodos: Se estudiaron los índices de evaluación del entrenamiento físico de los deportistas mediante la comprobación de la literatura científica y la estadística matemática, y se diseñó y experimentó un índice de evaluación del entrenamiento físico de los jóvenes jugadores de baloncesto. Resultados: La proporción más alta de la puntuación de la aptitud física especial en la media atlética fue del 46,25%; la proporción de la puntuación de la aptitud física general de los jugadores representó un 15% por encima y un 13,75% por debajo, el nivel de aptitud física específica de los jugadores de baloncesto está principalmente en el nivel medio. Conclusión: Los efectos del entrenamiento pueden evaluarse eficazmente mediante el sistema de índice de experiencia, y es útil para que los deportistas encuentren las deficiencias, aprovechen al máximo su propio potencial, mejoren las habilidades y adapten las tácticas de baloncesto. También puede proporcionar más talentos de reserva para el baloncesto profesional. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción Objetivo Métodos matemática Resultados 46,25% 4625 46 25 15 1375 13 75 13,75 debajo medio Conclusión experiencia deficiencias profesional II tratamiento 46,25 462 4 2 1 137 7 13,7 46,2 13, 46,

ABSTRACT Introduction: The training should be performed according to the characteristics of basketball by establishing a scientific evaluation index system to fully exploit the potential of its players. Objective: Evaluate the index system for measuring physical training specific to basketball players. Methods: The physical training evaluation indexes of athletes were studied by checking the scientific literature and mathematical statistics, and an evaluation index for the physical training of young basketball players was designed and experimented. Results: The highest proportion of the special fitness score in athletic average was 46.25%; the proportion of the players’ general fitness score represented 15% above and 13.75% below. The specific fitness level of basketball players is mainly at the average level. Conclusion: The effects of training can be effectively evaluated by the experienced index system, and it is useful for athletes to find deficiencies, make full use of their potential, improve skills and adapt basketball tactics. It may also provide more reserve talent for professional basketball. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction Objective Methods statistics experimented Results 46.25% 4625 46 25 15 1375 13 75 13.75 below Conclusion deficiencies tactics II outcomes 46.25 462 4 2 1 137 7 13.7 46.2 13. 46.

RESUMO Introdução: O treinamento deve ser realizado de acordo com as características do basquetebol estabelecendo um sistema de índice de avaliação científica para explorar plenamente o potencial de seus jogadores. Objetivo: Avaliar o sistema de índices de medição do treinamento físico específico para os jogadores de basquetebol. Métodos: Os índices de avaliação do treinamento físico dos atletas foram estudados verificando a literatura científica e estatísticas matemáticas, um índice de avaliação para o treinamento físico de jovens jogadores de basquetebol foi projetado e experimentado. Resultados: A maior proporção da pontuação da aptidão física especial na média atlética foi de 46,25%; a proporção da pontuação da aptidão física geral dos jogadores representou 15% acima e 13,75% abaixo, o nível de aptidão física específica dos jogadores de basquetebol está principalmente no nível mediano. Conclusão: Os efeitos do treinamento podem ser efetivamente avaliados pelo sistema de índice experimentado, sendo útil para que os atletas possam encontrar deficiências, aproveitar plenamente seu próprio potencial, melhorar as habilidades e adaptar as táticas do basquetebol. Também poderá fornecer mais talentos de reserva para o basquetebol profissional. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução Objetivo Métodos matemáticas experimentado Resultados 46,25% 4625 46 25 15 1375 13 75 13,75 abaixo mediano Conclusão deficiências profissional II tratamento 46,25 462 4 2 1 137 7 13,7 46,2 13, 46,

Abstract To explore the effects and mechanisms of benzoylaconitine and paeoniflorin on collagen-induced arthritis (CIA) rats. Weight, paw swelling, arthritis index and joint pathologic changes were examined in each group after CIA induction. PGE2, IL-1β, IL-6, IL-10, TNF-α, VEGF, MMP-3, IgG and anti-CII Ab were assessed by ELISA; STAT1 and STAT3 expressions were analyzed immunohistochemically, and the ultrastructure of synovial cells was observed by transmission electron microscopy. Therapeutic effects were determined in CIA rats via injecting benzoylaconitine and paeoniflorin, which could alleviate the degree of swelling and arthritis index (AI) and pathological lesions of the sacroiliac gland; decrease the levels of PGE2, IL-1β, TNF-α, VEGF and IgG in serum; reduce STAT1 and STAT3 expression in the membrane tissue; and inhibit the secretion and proliferation of synovial cells. These results showed that benzoylaconitine and paeoniflorin could significantly palliate the arthritic symptoms of CIA rats, and better therapeutic effects could be achieved if the two components were used in combination.

ABSTRACT Background: Clostridiodes difficile infection (CDI) is one of the most common causes of antibiotic-associated diarrhea in children. Conventional antibiotics and emerging fecal micro-biota transplantation (FMT) are used to treat CDI. Methods: Children with CDI admitted to the Shanghai Children’s Hospital, from September 2014 to September 2020, were retrospectively included to this observational study. Pediatric patients were assigned as initial CDI and recurrent CDI (RCDI), and symptoms, comorbid-ities, imaging findings, laboratory tests, and treatments were systematically recorded and analyzed. Results: Of 109 pediatric patients with CDI, 58 were boys (53.2%), and the median age was 5 years (range, 2-9 years). The main clinical symptoms of CDI children were diarrhea (109/109, 100%), hematochezia (55/109, 50.46%), abdominal pain (40/109, 36.70%); fever, pseudomembrane, vomit, and bloating were observed in 39 (35.78%), 33 (30.28%), and 24 (22.02%) patients, respectively. For the primary therapy with conventional antibiotics, 68 patients received metronidazole, and 41 patients received vancomycin. RCDI occurred in 48.53% (33/68) of those initially treated with metronidazole compared with 46.33% (19/41) of those initially treated with vancomycin (p=0.825). The total resolution rate of FMT for RCDI children was significantly higher than with vancomycin treatment (28/29, 96.55% vs 11/23, 47.83%, p < 0.001). There were no serious adverse events (SAEs) reported after two months of FMT. Conclusions: The major manifestations of children with CDI were diarrhea, hematochezia, and abdominal pain. The cure rate of FMT for pediatric RCDI is superior to vancomycin treatment.

ABSTRACT Purpose: Tanshinone IIA is a well-known lipophilic active constituent refined from traditional Chinese medicines, danshen. It has been previously demonstrated to possess various biological properties, including anti-inflammatory, antioxidant, promoting angiogenesis effect and so on. However, the mechanism of tanshinone IIA on myocardial ischemia-reperfusion injury (MI/RI) remains unclear. In this study, we investigated the effect of tanshinone IIA on MI/RI. Methods: MI/RI rat models were set up. Echocardiographic evaluation and hematoxylin and eosin staining were performed to analyze the cardiac function and morphology of MI/RI. Western blot was conducted for the detection of protein expression of pyrin domain containing 3 (NLRP3) and caspase-1 in heart tissues. Flow cytometry and real-time polymerase chain reaction were used for the detection of proinflammatory cytokines and Th17 cells differentiation. Results: We found that tanshinone IIA alleviated the myocardial damage of MI/RI, ameliorated the overall and local inflammatory reaction, and produced a cardioprotective effect by inhibiting of NLRP3 inflammasome activation and Th17/Treg cells differentiation. Conclusions: Our results highlighted the cardio-protective effect of tanshinone IIA on MI/RI and uncovered its underlying mechanism related to the NLRP3 inflammasome inhibition and the modulation of Th17/Treg cells differentiation.

Abstract Kernel direct harvesting is the mainstream technology of maize harvesting in the world today, it has significant impact on the maize kernel subsequent food processing. Direct harvest technology in china is not well developed due to the influence of growth environment, agronomy, etc., which leads kernel damage. The kernel damage is necessary studied in maize direct harvesting technology. Therefore, “Zheng Dan 958” was selected, the entrance clearance, export clearance, and cylinder speed as variables to carry out the kernel damage experiment. Processed the image of threshed maize kernel, extracted the crack and boundary characteristics of kernel damage, and established the BP neural network model to study the direct harvesting damage and optimize parameters. The results indicated that kernel damage increased with decreasing threshing clearance and increasing threshing intensity. In a certain threshing clearance, cylinder speed was the key factor affecting kernel damage. The R of model was above 0.95, the accuracy of damage quantitative identification was above 85%. When inlet clearance was 35 mm, outlet clearance was 15 mm, and cylinder speed was 300 rpm, kernel damage was small. Our findings will provide reference for kernel direct harvesting technology and improve harvest quality to meet food processing industry demands.