Abstract Phenylketonuria (PKU) is an autosomal recessive defect affecting the metabolic pathway of phenylalanine (Phe), causing hyperphenylalaninemia and neurotoxicity. Diagnosis must occur in the neonatal period and treatment should begin as early as possible. Evidence implies that treatment adherence declines as age advances. The aim was to describe the diet of a subgroup of Chilean adults with PKU currently in follow-up. Fifty-three subjects (49% women) followed up between January 2021 to April 2023 were considered. The concentration of Phe (PheC) in dried blood spots measured by fluorometry and 24-hour dietary recalls were analyzed. The median PheC of the sample was 438µmol/L (interquartile range(IQR):351-585µmol/L). A protein intake of 1.35±0.3 gr/Kg/d was observed of which 87% came from the protein substitute without Phe. Participants had a median Phe intake of 459mg/d (IQR:327-976) and 13.1g/d of fiber intake. Most participants, 51% and 92% reported consuming fruits and vegetables, respectively, and 32% consumed Low-Protein foods. Regarding micronutrients, all participants exceeded 90% adequacy according to recommendations. For vitamin-D and vitamin-B12, 100% is provided by the protein substitute. According to our results, it is mandatory to establish transition programs toward adulthood, to constantly maintain good metabolic control, and to adapt diet therapy to their new lifestyle. (PKU Phe, , (Phe) neurotoxicity possible advances followup. followup follow up. follow-up Fiftythree Fifty three 49% 49 (49 women 202 considered (PheC 24hour hour 24 analyzed 438µmolL µmolL 438µmol L µmol interquartile rangeIQR351585µmol/L. rangeIQR351585µmolL rangeIQRµmolL range IQR 351 585µmol/L . 585µmol range(IQR):351-585µmol/L) 13503 1 35 0 3 1.35±0. grKgd gr Kg d 87 459mgd mgd 459mg mg IQR327976 327 976 (IQR:327-976 131gd gd 13 1g g 51 92 vegetables respectively 32 LowProtein Low Protein foods micronutrients 90 recommendations vitaminD vitamin D vitaminB12, vitaminB12 vitaminB B12, B12 B vitamin-B12 100 results adulthood control lifestyle (Phe 4 (4 20 2 rangeIQR351585µmol rangeIQR rangeIQR351585µmol/L 585µmolL range(IQR):351-585µmol/L 1350 1.35±0 8 IQR32797 97 (IQR:327-97 5 9 vitaminB1 B1 vitamin-B1 10 ( rangeIQRµmol 135 1.35± IQR3279 (IQR:327-9 vitamin-B 1.35 IQR327 (IQR:327- 1.3 IQR32 (IQR:327 1. IQR3 (IQR:32 (IQR:3 (IQR: (IQR

In this study, a mixed oxide (SiO2/ZnO/Fe2O3/ZnFe2O4) was prepared using the anode recycled from alkaline batteries and the silt fraction of mining tailings and applied as a photocatalyst under solar irradiation and as a pseudocapacitor. The structure and morphology of the mixed oxide revealed clusters of nanometric particles with rhombic and octahedral shapes. A 23 full factorial design was performed to determine the effect of pH, hydrogen peroxide (H2O2), and photocatalyst on methylene blue (MB) decolorization. The maximum efficiency was 96%, obtained using 2.35 × 10-5 mol H2O2 and 20 mg of photocatalyst at pH 3 for 30 min under solar irradiation. This efficiency remained even after four successive decolorization cycles. Pseudocapacitive properties showed that the mixed oxide exhibited favorable characteristics for application as electrochemical pseudocapacitors due to its high capacitance (87.16 F g-1), energy density (27.24 Wh kg-1) at 524.41 W kg-1 of power density, and reversibility (93%). The studied mixed oxide has excellent potential for use in photocatalytic reactions and electrochemical devices, contributing to environmental sustainability and circular economy. study SiO2/ZnO/Fe2O3/ZnFe2O4 SiO2ZnOFe2O3ZnFe2O4 SiOZnOFeOZnFeO SiO2 ZnO Fe2O3 ZnFe2O4 SiO Fe O ZnFe (SiO2/ZnO/Fe2O3/ZnFe2O4 pseudocapacitor shapes 2 H2O2, HO , H (H2O2) MB (MB 96 96% 235 35 2.3 105 10 5 10- H2O cycles 87.16 8716 87 16 (87.1 g1, g1 g 1 g-1) 27.24 2724 27 24 (27.2 kg1 kg 52441 524 41 524.4 kg- 93%. 93 93% . (93%) devices economy SiO2/ZnO/Fe2O3/ZnFe2O ZnOFe SiO2ZnOFe2O3ZnFe2O FeO Fe2O ZnFeO ZnFe2O (SiO2/ZnO/Fe2O3/ZnFe2O (H2O2 9 2. 87.1 871 8 (87. g-1 27.2 272 (27. 5244 52 4 524. (93% (H2O 87. (87 g- 27. (27 (93 (8 (2 (9 (

ABSTRACT Bell pepper is a globally important vegetable. The use of humic substances can enhance nutrient absorption efficiency, reducing the amounts of organic residues applied to the soil. Therefore, the objective of this study was to evaluate cattle manure rates combined with humic substance rates to increase bell pepper production. A randomized block design with four replicates was used, in a 4 × 3 factorial arrangement consisted of four cattle manure doses (8, 18, 28, and 38 Mg ha-1) and three humic substance doses (0, 8, and 12 kg ha-1). Humic substances affected bell pepper production characteristics; the highest mean fruit weight per plant (140.02 g) and fruit diameter (75.73 mm) were obtained when applying 8 Mg ha-1 of manure combined with 8 kg ha-1 of humic substances. Similarly, the greatest fruit length (80.94 mm) was found when applying 8 Mg ha-1 of manure combined with 12 kg ha-1 of humic substances. The highest yields (1.2 kg per plant and 60.14 Mg ha-1) were obtained when applying 38 Mg ha-1 of manure combined with 12 kg ha-1 of humic substances. The combination of humic substances and cattle manure increased the organic production of bell peppers under the edaphoclimatic conditions of the Semiarid region of Brazil. vegetable efficiency soil Therefore used (8 18 28 ha1 ha 1 0, 0 (0 ha1. . characteristics 140.02 14002 140 02 (140.0 g 75.73 7573 75 73 (75.7 mm ha- Similarly 80.94 8094 80 94 (80.9 1.2 2 (1. 6014 60 14 60.1 Brazil ( 140.0 1400 (140. 75.7 757 7 (75. 80.9 809 9 (80. 1. (1 601 6 60. 140. (140 75. (75 80. (80 (14 (7

RESUMO O pimentão é uma olerícola de importância mundial. O uso de substâncias húmicas pode elevar a eficiência de absorção nutricional reduzindo a quantidade de resíduos orgânicos aplicados no solo. Assim sendo, objetivou-se avaliar doses de esterco bovino associadas a substâncias húmicas para elevar a produção de pimentão. O delineamento em blocos casualizados distribuído em arranjo fatorial com quatro doses de esterco bovino (8, 18, 28 e 38 t ha-1) e três doses de substâncias húmicas (0, 8 e 12 kg ha-1), quatro repetições foi utilizado. As substâncias húmicas influenciaram nas características produtivas, obtendo-se valores máximos de peso médio de fruto (140,02 g), diâmetro de fruto (75,73 mm) aplicando 8 t ha-1 de esterco associado a 8 g ha-1 de substâncias húmicas, como também, obteve-se valor máximo de comprimento de fruto (80,94 mm) aplicando 8 t ha-1 de esterco associado a 12 kg ha-1 de substâncias húmicas. A produção máxima de 1,2 kg por planta e 60,14 t ha-1, obteve-se aplicando 38 t ha-1 de esterco associado a 12 kg ha-1 de substâncias húmicas. A associação de substâncias húmicas com esterco bovino aumentou a produção orgânica de pimentão nas condições edafoclimáticas do Semiárido. mundial solo sendo objetivouse objetivou se 8, (8 18 2 3 ha1 ha 1 0, 0 (0 ha1, , utilizado produtivas obtendose obtendo 140,02 14002 140 02 (140,0 g, g) 75,73 7573 75 73 (75,7 mm ha- também obtevese obteve 80,94 8094 80 94 (80,9 1, 6014 60 14 60,1 Semiárido ( 140,0 1400 (140, 75,7 757 7 (75, 80,9 809 9 (80, 601 6 60, 140, (140 75, (75 80, (80 (14 (7 (1

ABSTRACT This was the first study conducted on the diversity and abundance of arbuscular mycorrhizal fungi (AMF) species and quantification of glomalin-related soil protein (GRSP) fractions, as well as on their relationship with environmental and soil chemical and physical properties along an elevation gradient above 1000 m in a Brazilian Atlantic cloud forest. AMF diversity was assessed by examining spores extracted from soil samples collected near the roots of the 18 most common plant species in permanent plots established in the field at altitudes of 1500, 1700, 1900, and 2100 meters above sea level. Fifteen AMF species were found, most of them belonging to the families Acaulosporaceae, Glomeraeceae, and Gigasporaceae. Compositions of the AMF community varied among the altitudes; Acaulospora was the predominant genus, with six species. The total Bradford-reactive soil protein (BRSP) and the easily extractable BRSP (EE-BRSP) soil glomalin were the highest at the altitude of 2100 m (5.7 and 3.8 mg.g soil-1, respectively). Altitude and environment and soil characteristics affected the composition and diversity (Shannon index) of the AMF communities. However, the effect of altitude on AMF diversity can be estimated, indirectly, through its effect on plant diversity. (AMF glomalinrelated related GRSP (GRSP fractions 100 forest 1 1500 1700 1900 210 level found Acaulosporaceae Glomeraeceae Gigasporaceae genus Bradfordreactive Bradford reactive (BRSP EEBRSP EE (EE-BRSP 5.7 57 5 7 (5. 38 3 8 3. mgg mg g soil1, soil1 1, soil-1 respectively. respectively . respectively) Shannon index communities However estimated indirectly 10 150 170 190 21 5. (5 soil- 15 17 19 2 (

Abstract Thiosemicarbazones are promising classes of compounds with antitumor activity. For this study, six 2,4-dihydroxy-benzylidene-thiosemicarbazones compounds were synthesized. These compounds were submitted to different assays in silico, in vitro and in vivo to evaluate the toxicological, antioxidant and antitumor effects. The in silico results were evaluated by the SwissADME and pkCSM platforms and showed that all compounds had good oral bioavailability profiles. The in vitro and in vivo toxicity assays showed that the compounds showed low cytotoxicity against different normal cells and did not promote hemolytic effects. The single dose acute toxicity test (2000 mg/kg) showed that none of the compounds were toxic to mice. In in vitro antioxidant activity assays, the compounds showed moderate to low activity, with PB17 standing out for the ABTS radical capture assay. The in vivo antioxidant activity highlighted the compounds 1, 6 and 8 that promoted a significant increase in the concentration of liver antioxidant enzymes. Finally, all compounds showed promising antitumor activity against different cell lines, especially MCF-7 and DU145 lines, in addition, they inhibited the growth of sarcoma 180 at concentrations lower than 50 mg/kg. These results showed that the evaluated compounds can be considered as potential antitumor agents. study 2,4dihydroxybenzylidenethiosemicarbazones 24dihydroxybenzylidenethiosemicarbazones dihydroxybenzylidenethiosemicarbazones 2,4 dihydroxy benzylidene thiosemicarbazones 2 4 synthesized toxicological effects profiles 2000 (200 mg/kg mgkg mg kg mice PB PB1 assay 1 enzymes Finally lines MCF7 MCF 7 MCF- DU DU14 addition 18 5 agents 4dihydroxybenzylidenethiosemicarbazones 24 2, 200 (20 DU1 20 (2 (

Abstract Background: Conventional dental care is often impossible in patients with Autism Spectrum Disorder (ASD). Non-collaborative behaviors, sometimes associated with aggressiveness, are usual justifications for premedication in this population. Thereby, this research focuses on the effects of oral midazolam versus oral ketamine plus midazolam as preanesthetic medication in ASD. Methods: The sample included 64 persons with ASD, aged 2-59 years, scheduled for dental care under general anesthesia. The primary objective of this study was to compare degrees of sedation between two parallel, double-blinded, equally proportional groups randomized to receive oral midazolam (0.5 mg.kg−1, maximum 15 mg) or oral midazolam (0.5 mg.kg−1) associated with oral S(+)-ketamine (3 mg.kg−1, maximum 300 mg). The secondary outcomes were the need of physical stabilization to obtain intravenous line, awakening time, and occurrence of adverse events. Results: According to the dichotomous analysis of sedation level (Ramsay score 1 and 2 versus Ramsay ≥ 3), oral association of S(+)-ketamine and midazolam improved sedation, with increased probability of Ramsay ≥ 3, Relative Risk (RR) = 3.2 (95% Confidence Interval [95% CI] = 1.32 to 7.76) compared to midazolam alone. Combined treatment also made it easier to obtain venous access without physical stabilization, RR = 2.05 (95% CI = 1.14 to 3.68). There were no differences between groups regarding awakening time and the occurrence of adverse events. Conclusion: The association of oral S(+)-ketamine with midazolam provides better preanesthetic sedation rates than midazolam alone and facilitates intravenous line access in patients with autism. Background ASD . (ASD) Noncollaborative Non collaborative behaviors aggressiveness population Thereby Methods 6 259 59 2-5 years anesthesia parallel doubleblinded, doubleblinded double blinded, blinded double-blinded 0.5 05 0 5 (0. mgkg1 mgkg mg kg mg.kg−1 S+ketamine Sketamine S + 3 ( 30 mg. events Results , 3) (RR 32 3. 95% 95 (95 [95 132 1.3 7.76 776 7 76 205 2.0 114 14 1.1 3.68. 368 3.68 68 3.68) Conclusion autism (ASD 25 2- 0. (0 mg.kg− 9 (9 [9 13 1. 7.7 77 20 2. 11 36 3.6 mg.kg [ 7.

ABSTRACT. Ractopamine is a drug used as an additive in animal production to improve growth, body composition, and production efficiency, although its use in fish is still poorly known. This study aimed to evaluate ractopamine hydrochloride (RAC) effects at increasing levels (0, 10, 20, and 40 mg kg−1) in Nile tilapia (Oreochromis niloticus) diets during 40 days by considering growth performance, body composition, and muscle fiber morphometry. One hundred and twelve fish (mean initial weight 518.63 ± 5.49 g) were housed in 16 circular tanks (450 L), following a completed randomized design with four treatments and four replicates. Body composition was significantly affected (P < 0.05), and the content of lipids in fish fillets dropped 26.7% (20 mg kg−1 RAC) and 26.1% (40 mg kg−1 RAC), as well as the amount of unsaturated fatty acids C14:0 and C18:0 in fish fillets (P < 0.05) by treatment with 10 mg kg−1 of RAC. Results demonstrate that fish fed 20 mg kg−1 RAC for 40 days changed Nile tilapia fillet chemical composition, decreasing lipid levels, altering the fatty acid profile, and increasing protein levels. RAC did not affect any productive performance parameters evaluated for muscle fiber morphometry.

Abstract Faced with changes in eating habits, consumers currently seek to enjoy moderate consumption of healthy food products that results in sensory pleasure. Here, our study aimed to evaluate the biochemical and physicochemical properties and sensory profile of craft beers produced in the Brazilian Midwest region. Our evaluation of the beverages revealed different physicochemical characteristics such as alcohol content, soluble solids, titratable acidity in citric acid, and dry extract percentage, with values ranging between 4.06-6.13%, 5.13-10.98 °Brix, 1.71-3.45%, and 3.38-7.99%, respectively. Moreover, the average density of the beverages was 0.9906 g/cm3. In addition, the antioxidant activity and total phenolic of the beverages ranged between 14.28-20.46 mMol of Trolox/100 mL and 74.84-108.45 mg/100 g, respectively. Phenolic compounds that are essential in human nutrition, such as epicatechin, catechin, gallic acid, p-coumaric acid, caffeic acid, ferulic acid, rutin, and kaempferol, were identified in the beverages. Regarding the sensory profile of the craft beers, the beers had an average acceptability index of 75.11% for appearance, 70.24% for aroma, 60.43% for flavor, and 73.53% for texture. Our study demonstrated a correlation between chemical composition and acceptability index of beer, indicating a preference for lighter colored, fuller, and less acidic beers. habits pleasure Here region content solids acid percentage 4.066.13%, 406613 4.06 6.13%, 4 06 6 13 4.06-6.13% 5.1310.98 5131098 5.13 10.98 5 10 98 5.13-10.9 Brix °Brix 1.713.45%, 171345 1.71 3.45%, 1 71 3 45 1.71-3.45% 3.387.99%, 338799 3.38 7.99%, 38 7 99 3.38-7.99% respectively Moreover 09906 0 9906 0.990 gcm3 gcm g cm3 cm g/cm3 addition 14.2820.46 14282046 14.28 20.46 14 28 20 46 14.28-20.4 Trolox100 Trolox 100 Trolox/10 74.84108.45 748410845 74.84 108.45 74 84 108 74.84-108.4 mg100 mg mg/10 nutrition epicatechin catechin pcoumaric p coumaric rutin kaempferol 7511 75 11 75.11 appearance 7024 70 24 70.24 aroma 6043 60 43 60.43 flavor 7353 73 53 73.53 texture beer colored fuller 066 4.066.13% 40661 406 4.0 613 6.13% 4.06-6.13 1310 5.1310.9 513109 513 5.1 1098 10.9 9 5.13-10. 713 1.713.45% 17134 171 1.7 345 3.45% 1.71-3.45 387 3.387.99% 33879 338 3.3 799 7.99% 3.38-7.99 0990 990 0.99 g/cm 2820 14.2820.4 1428204 1428 14.2 2046 20.4 2 14.28-20. Trolox10 Trolox/1 84108 74.84108.4 74841084 7484 74.8 10845 108.4 8 74.84-108. mg10 mg/1 751 75.1 702 70.2 604 60.4 735 73.5 4.066.13 4066 40 4. 61 6.13 4.06-6.1 131 5.1310. 51310 51 5. 109 10. 5.13-10 1.713.45 1713 17 1. 34 3.45 1.71-3.4 3.387.99 3387 33 3. 79 7.99 3.38-7.9 099 0.9 282 14.2820. 142820 142 14. 204 20. 14.28-20 Trolox1 Trolox/ 8410 74.84108. 7484108 748 74. 1084 108. 74.84-108 mg1 mg/ 75. 70. 60. 73. 4.066.1 6.1 4.06-6. 5.1310 5131 5.13-1 1.713.4 3.4 1.71-3. 3.387.9 7.9 3.38-7. 09 0. 14.2820 14282 14.28-2 841 74.84108 748410 74.84-10 4.066. 6. 4.06-6 5.131 5.13- 1.713. 1.71-3 3.387. 7. 3.38-7 14.282 14.28- 74.8410 74841 74.84-1 4.066 4.06- 1.713 1.71- 3.387 3.38- 74.841 74.84-

Abstract This study aimed to determine whether the addition of a microencapsulated herbal blend (MHB) based on thymol, carvacrol, and cinnamaldehyde in dairy sheep feed would improve production efficiency, milk quality, and animal health. Thirty lactating Lacaune ewes were divided into three groups: Control (T0), 150 mg blend/kg of feed (T150), and 250 mg blend/kg of feed (T250). Milk was measured before the beginning of the experiment (d 0), at the end of the adaptation period (d 15), and during the experiment (d 20). In milk samples, was measured the composition, somatic cell count (SCC), reactive oxygen species (ROS), lipoperoxidation (LPO), and total antioxidant capacity. The MHB improved the milk production (only T150 vs. T0 sheep on d 20), productive efficiency and feed efficiency, and reduced the milk SCC (only T250 vs. T0 sheep, on d 20), ROS and tended to reduce the milk levels of LPO (only T250 vs. T0 sheep on d 20). Also, MHB reduced the blood levels of neutrophils and ROS (only T250 vs. T0 sheep on d 20) and increased total protein and globulin levels. Thus, a microencapsulated blend of thymol, carvacrol, and cinnamaldehyde improved the productive performance and milk quality of sheep. (MHB thymol carvacrol health groups T0, T , (T0) 15 blendkg kg T150, (T150) 25 T250. . (T250) 0, 0 0) 15, 15) 20. 20 samples composition SCC, (SCC) ROS, (ROS) LPO, (LPO) capacity only T15 vs 20, T25 Also Thus (T0 1 (T150 2 (T250 (SCC (ROS (LPO T1 T2 (T (T15 (T25 (T1 (T2

ABSTRACT. This study was performed to investigate the effects of composted tannery sludge (CTS) on the physical properties of tropical sandy soil after seven years of CTS application. CTS was applied to a Fluvisol at five rates (0.0, 2.5, 5.0, 10.0, and 20.0 Mg ha-1) in experimental plots (sized 20 m2) with four replications. Water infiltration into the soil was determined in the field with the concentric-ring infiltrometer method. Bulk density, total porosity, macroporosity, and microporosity were determined in the soil samples. The permanent CTS application altered the physical properties of the soil and led to a decrease in bulk density. The total porosity, microporosity and macroporosity values in the CTS-applied soil ranged from 44.1-51.7, 34.6-39.4, and 9.1-12.8%, respectively. Water-infiltration rates were significantly influenced by CTS. The cumulative infiltrated water in the soil varied from 21.3-34.7 cm. The basic infiltration rate was lower in the unamended soil and increased with an increase in the rate of CTS application. This study confirmed that the physical soil parameters improved after the permanent CTS application. Therefore, this application may be a suitable strategy for improving physical soil properties over time. ABSTRACT (CTS 0.0, 00 0 (0.0 25 2 5 2.5 50 5.0 100 10 10.0 200 20. ha1 ha 1 ha-1 sized m2 m replications concentricring concentric ring method density porosity samples CTSapplied 44.151.7, 441517 44.1 51.7, 44 51 7 44.1-51.7 34.639.4, 346394 34.6 39.4, 34 6 39 4 34.6-39.4 9.112.8%, 91128 9.1 12.8%, 9 12 8 9.1-12.8% respectively Waterinfiltration 21.334.7 213347 21.3 34.7 21 3 21.3-34. cm Therefore time 0.0 (0. 2. 5. 10. ha- 151 44.151.7 44151 441 44. 517 51.7 44.1-51. 639 34.639.4 34639 346 34. 394 39.4 34.6-39. 112 9.112.8% 9112 91 9. 128 12.8% 9.1-12.8 334 21.334. 21334 213 21. 347 21.3-34 0. (0 15 44.151. 4415 51. 44.1-51 63 34.639. 3463 39. 34.6-39 11 9.112.8 911 12.8 9.1-12. 33 21.334 2133 21.3-3 ( 44.151 44.1-5 34.639 34.6-3 9.112. 12. 9.1-12 21.33 21.3- 44.15 44.1- 34.63 34.6- 9.112 9.1-1 9.11 9.1-

ABSTRACT The paper processing industry is characterized as one of the three largest producers of wastewater of significant environmental contamination. In order to minimize impacts on water resources and comply with the standards required by legislation, advanced treatment solutions are needed. The present study developed a ceramic microfiltration membrane based on 60% of rice husk ash waste and 40% of kaolinitic clay to pretreat a raw effluent of paper industries. The membrane was obtained by uniaxial compression (27MPa) in an electric press, with subsequent sintering at 1,220°C for 1 hour. Base formulation materials were characterized by chemical and physical analyses. The membrane presented apparent porosity of 34.59%, density of 1,30 g.cm-3, diameter and thickness linear retraction of 1.7 and 2.5%, respectively, fire loss of 9.3% and a good chemical stability at acid and base solution. Hydraulic permeability corresponded to 11.3 × 10-3 m3/m2 s MPa-1, with a hydraulic pore diameter to 2.29 μm and average pore size of 1.727 μm. The characterization of the raw effluent showed high levels of COD (1,125.4 mg.L-1), turbidity (1,300 UNT), and color (253.8 UC). The filtration study indicated good results of membrane efficiency, with removal of turbidity (99.3%), COD (95.7%), color (83.0%), phenolic compounds (82.1%), and solids (85.1 to 90%). The rice husk ash accomplished the function as pore-form agent and adsorbent of phenolic compounds and represented a promising alternative for incorporating the waste into the production cycle. contamination legislation needed 60 40 industries 27MPa MPa (27MPa press 1220C C 220 hour analyses 3459 34 59 34.59% 130 30 1,3 g.cm3, gcm3 gcm g.cm 3, g cm 3 g.cm-3 17 7 1. 25 2 5 2.5% respectively 93 9 9.3 solution 113 11 11. 103 10 10- m3m2 mm m3 m2 m m3/m MPa1, MPa1 1, MPa-1 229 29 2.2 1727 727 1.72 1,125.4 11254 125 4 (1,125. mg.L1, mgL1 mgL mg.L , mg L mg.L-1) 1,300 1300 300 (1,30 UNT, UNT UNT) 253.8 2538 253 8 (253. UC. UC . UC) efficiency 99.3%, 993 99.3% 99 (99.3%) 95.7%, 957 95.7% 95 (95.7%) 83.0%, 830 83.0% 83 0 (83.0%) 82.1%, 821 82.1% 82 (82.1%) 85.1 851 85 (85. 90%. 90 90% 90%) poreform form cycle 6 22 345 34.59 13 cm3 g.cm3 g.cm- 2.5 9. m3m MPa- 2. 172 72 1,125. 1125 12 (1,125 L1 mg.L1 mg.L-1 (1,3 253. (253 99.3 (99.3% 95.7 (95.7% 83.0 (83.0% 82.1 (82.1% 85. (85 34.5 1,125 112 (1,12 mg.L- (1, (25 99. (99.3 95. (95.7 83. (83.0 82. (82.1 (8 34. 1,12 (1,1 (1 (2 (99. (95. (83. (82. ( 1,1 (99 (95 (83 (82 (9

RESUMO A indústria de processamento de papel está entre as três maiores produtoras de águas residuais de significativa contaminação ambiental. Com o objetivo de minimizar os impactos nos corpos d’água e se adequar aos padrões exigidos pela legislação, são necessárias soluções avançadas de tratamento. Neste trabalho, uma membrana cerâmica de microfiltração foi desenvolvida, tendo como base 60% da composição correspondente ao resíduo de cinza de casca de arroz e 40% de Caulim, para ser aplicada como pré-tratamento do efluente real da indústria de papel. A membrana foi obtida por compactação uniaxial a 27 MPa em uma prensa elétrica, com posterior sinterização em mufla a 1.200°C por 1 hora. Os materiais foram caracterizados por análises químicas e físicas. A membrana apresentou porosidade aparente de 34,59%, densidade de 1,30 g/cm³, retração linear de diâmetro e espessura de 1,7 e 2,5%, respectivamente, perda ao fogo de 9,3% e boa estabilidade química em ácido e base. A permeabilidade hidráulica correspondeu a 11,3 × 10-3 m3/m2 s MPa-1, com diâmetro hidráulico de poros de 2,29 μm e tamanho médio de poros de 1,727 μm. A caracterização do efluente bruto revelou altos teores de demanda química de oxigênio (1.125,4 mg•L-1), turbidez (1.300 UNT) e cor (253,8 UC). O estudo de filtração indicou resultados satisfatórios de eficiência da membrana, com remoção de turbidez (99,3%), demanda química de oxigênio (95,7%), cor (83,0%), compostos fenólicos (82,1%) e sólidos (85,1 a 90%). O uso da cinza de casca de arroz desempenhou a função de agente formador de poros e adsorvente de compostos fenólicos, representando promissora alternativa para a incorporação do resíduo ao ciclo produtivo. ambiental dágua d água legislação tratamento trabalho desenvolvida 60 40 Caulim prétratamento pré 2 elétrica 1200C C 200 hora físicas 3459 34 59 34,59% 130 30 1,3 gcm³ gcm g cm³ cm g/cm³ 17 7 1, 25 5 2,5% respectivamente 93 9 3 9,3 113 11 11, 103 10 10- m3m2 mm m3 m2 m m3/m MPa1, MPa1 MPa-1 229 29 2,2 1727 727 1,72 1.125,4 11254 125 4 (1.125, mg•L1, mgL1 mgL mg•L , mg L mg•L-1) 1.300 1300 300 (1.30 UNT 253,8 2538 253 8 (253, UC. UC . UC) 99,3%, 993 99,3% 99 (99,3%) 95,7%, 957 95,7% 95 (95,7%) 83,0%, 830 83,0% 83 0 (83,0%) 82,1% 821 82 (82,1% 85,1 851 85 (85, 90%. 90 90% 90%) produtivo 6 20 345 34,59 13 g/cm 2,5 9, m3m MPa- 22 2, 172 72 1.125, 1125 12 (1.125 L1 mg•L1 mg•L-1 1.30 (1.3 253, (253 99,3 (99,3% 95,7 (95,7% 83,0 (83,0% 82,1 (82,1 85, (85 34,5 1.125 112 (1.12 mg•L- 1.3 (1. (25 99, (99,3 95, (95,7 83, (83,0 82, (82, (8 34, 1.12 (1.1 1. (1 (2 (99, (95, (83, (82 ( 1.1 (99 (95 (83 (9

Abstract Background The high incidence of atrial arrhythmias in pulmonary hypertension (PH) might be associated with poor prognosis, and the left atrium (LA) may play a role in this. An important finding in PH studies is that LA remodeling is underestimated. Objective This study investigated LA morphology and mechanical function, as well as the susceptibility to develop arrhythmias in a monocrotaline-induced PH (MCT-PH) model. Methods Wistar rats aged 4 weeks received 50 mg/kg of MCT. Electrocardiography and histology analysis were performed to evaluate the establishment of the MCT-PH model. The tissue was mounted in an isolated organ bath to characterize the LA mechanical function Results Compared with the control group (CTRL), the MCT-PH model presented LA hypertrophy and changes in cardiac electrical activity, as evidenced by increased P wave duration, PR and QT interval in MCT-PH rats. In LA isolated from MCT-PH rats, no alteration in inotropism was observed; however, the time to peak contraction was delayed in the experimental MCT-PH group. Finally, there was no difference in arrhythmia susceptibility of LA from MCT-PH animals after the burst pacing protocol. Conclusion The morphofunctional remodeling of the LA did not lead to increased susceptibility to ex vivo arrhythmia after application of the burst pacing protocol. (PH prognosis (LA this underestimated monocrotalineinduced monocrotaline induced MCTPH MCT (MCT-PH 5 mgkg mg kg CTRL, CTRL , (CTRL) activity duration observed however Finally protocol (CTRL

Resumo Fundamento A alta incidência de arritmias atriais na hipertensão pulmonar (HP) pode estar associada a um prognóstico ruim, e o átrio esquerdo (AE) pode desempenhar um papel neste quadro. Um achado importante nos estudos de HP é que a remodelação do AE é subestimada. Objetivo Este estudo investigou a morfologia e a função mecânica do AE, bem como a suscetibilidade ao desenvolvimento de arritmias em um modelo de HP induzida por monocrotalina (HP-MCT). Métodos Ratos Wistar com 4 semanas de idade receberam 50 mg/kg de MCT. Foram realizadas análises eletrocardiográficas e histológicas para avaliar o estabelecimento do modelo de HP-MCT. O tecido foi montado em banho de órgão isolado para caracterizar a função mecânica do AE. Resultados Em comparação com o grupo controle, o modelo de HP-MCT apresentou hipertrofia do AE e alterações da atividade elétrica cardíaca, conforme evidenciadas pelo aumento da duração da onda P, PR e intervalo QT. Não foi observada alteração no inotropismo do AE isolado de ratos com HP-MCT; no entanto, o tempo para atingir a contração máxima foi atrasado. Finalmente, não observamos diferença na suscetibilidade à arritmia no AE dos ratos com HP-MCT após o protocolo de estimulação intermitente. Conclusão A remodelação morfofuncional do AE não levou ao aumento da suscetibilidade à arritmia ex vivo após a aplicação do protocolo de estimulação intermitente. (HP ruim (AE quadro subestimada HPMCT. HPMCT MCT . (HP-MCT) 5 mgkg mg kg controle cardíaca P QT entanto atrasado Finalmente intermitente (HP-MCT

Abstract Objectives Evidence suggests that ketamine’s influence on brain-derived neurotrophic factor (BDNF) might be involved in its mechanism of rapid antidepressant action. We aimed to evaluate the differential impact of ketamine and esketamine on serum BDNF levels and its association with response patterns in treatment-resistant depression (TRD). Methods Participants (n = 53) are from a randomized, double-blind clinical trial comparing the efficacy of single-dose ketamine (0.5mg/kg, n = 27) and esketamine (0.25mg/kg, n = 26) in TRD. Depression severity was assessed before and 24 hours, 72 hours, and 7 days after the intervention, using the Montgomery-Åsberg Depression Rating Scale (MADRS). Blood samples were collected before infusion, 24 hours, and 7 days afterwards. Results There were no significant changes in BDNF levels at post-infusion evaluation points, and no difference in BDNF levels comparing ketamine and esketamine. Both drugs exhibited similar therapeutic effect. There was no association between BDNF levels and response to treatment or severity of depressive symptoms. Conclusion There was no significant treatment impact on BDNF serum levels – neither with ketamine nor esketamine – despite therapeutic response. These results suggest that ketamine or esketamine intervention for TRD has no impact on BDNF levels measured at 24 hours and 7 days after the infusion. This clinical trial is registered on the Japan Primary Registries Network: UMIN000032355. ketamines s brainderived brain derived (BDNF action treatmentresistant resistant . (TRD) 53 randomized doubleblind double blind singledose single dose 0.5mg/kg, 05mgkg mgkg 0 5mg kg mg (0.5mg/kg 27 0.25mg/kg, 025mgkg 25mg (0.25mg/kg 26 2 MontgomeryÅsberg Montgomery Åsberg MADRS. MADRS (MADRS) infusion afterwards postinfusion post points effect symptoms Network UMIN000032355 UMIN (TRD 5 0.5mg/kg 0.25mg/kg (MADRS UMIN00003235 UMIN0000323 UMIN000032 UMIN00003 UMIN0000 UMIN000 UMIN00 UMIN0

ABSTRACT: Pornunça (Manihot sp.) is a potential forage to feed ruminants in drylands worldwide; however, evaluations of animal diets are necessary. This study assessed intake and digestibility of dry matter and nutrients, physiological responses, ingestive behavior, water intake, as well as hematological and biochemical responses of lambs fed diets containing increasing levels of Pornunça silage (PS) replacing Tifton-85 bermudagrass hay (Cynodon spp.). Treatments consisted of 0, 33, 66, and 100 % PS considering the roughage portion of the diet (% dry matter – DM). The experimental design was completely randomized with six replicates. Cyanide acid (HCN) levels in fresh Pornunça leaves were 207.7 mg kg−1 DM and 76.78 mg kg−1 DM in PS. Intake of DM and crude protein (CP), and neutral detergent fiber corrected for ash and protein (NDFap) increased linearly with increasing levels of PS in the diet. Digestibility of DM, NDF, and CP were higher with increasing levels of PS. Idle time decreased linearly, while water balance, water intake via food, and total water in feces increased with PS. Nitrogen balance, physiological, and hematological responses were not influenced by treatments, while the final alanine aminotransferase increased for lambs fed all diets evaluated. The PS up to 100 % of the roughage portion increased DM and nutrient intake and digestibility without altering physiological responses and the hematological and biochemical parameters, resulting in greater water intake via food.

Abstract: Groundwater discharges (GD) provide information for understanding the local hydrogeology of a region. This study comprehensively describes a GD in the coast of Quintana Roo, quantifying the volumetric discharge and describing the hydrochemistry and geophysics of the site in order to contribute to the knowledge of the coastal karst aquifer in Quintana Roo. The GD described is an oval opening with an approximate area of 0.0507 m2 where major elements, silicates, metals and stable isotopes of water (δ2H and δ18O) were quantified monthly. The contribution of the accumulated monthly rainfall (Qp) in the discharge flow was calculated; electrical resistivity tomography was performed to identify saturated zones and flow zones. In general, Na, K, Ca, Mg, and Cl tend to decrease as Qp increases. SiO2, ORP and salinity do not show clear relations with changes in Qp, only in some sampling events there was a positive relationship, while the rest of the time is negative or null. The water is sodium-potassium-chloride type. The contribution of groundwater dominates, seawater contributes between 2 and 16% of the flow. The greatest contribution of seawater is observed when the flow of the GD is low. The contribution of Qp is between 1 and 15% and is not measured immediately. Geophysics survey suggest the existence of a lens of free fresh water approximately 2.5 m thick and recharge zones, as well as the existence of small dissolution channels where groundwater flows. The results capture local and regional aquifer recharge events.

Resumen: Las descargas de agua subterránea (DAS) aportan información para entender la hidrogeología local dentro de una región. El objetivo de este estudio fue describir de manera integral una DAS en la zona costera de Quintana Roo para contribuir al conocimiento del acuífero kárstico costero en Quintana Roo. La DAS es una cavidad ovalada con un área aproximada de 0.0507 m2 donde mensualmente se cuantificaron elementos mayores, silicatos, metales e isótopos estables del agua (δ2H y δ18O). Se calculó el aporte de la precipitación pluvial mensual acumulada (Qp) en el caudal de la descarga, se realizaron tomografías de resistividad eléctrica para identificar zonas saturadas y zonas de flujo. En lo general el Na, K, Ca, Mg y Cl tendieron a disminuir cuando Qp aumenta. SiO2, ORP y salinidad no tuvieron una relación evidente con Qp durante el tiempo de medición. El agua es de tipo clorurada sódico potásica; domina el aporte de agua subterránea, el agua de mar contribuyó entre 2 y 16% del caudal. El mayor aporte del agua de mar se observó cuando el caudal de la DAS es bajo. La contribución de Qp fue de entre y 15% y no se observó de manera inmediata. La geofísica apunta a un lente de agua dulce libre de aproximadamente 2.5 m de espesor y zonas de recarga, así como la existencia de pequeños canales de conducción por donde fluye el agua subterránea. Los resultados capturan eventos locales y regionales de recarga del acuífero.