Abstract Since the inception of COVID-19 pandemic, there has been a challenging race for the development of precise diagnostic tests. Specific SARS-CoV-2 serological assays are the main tools used to estimate the rate of past infections or herd immunity in epidemiological studies, in addition to being helpful in guiding public health management policies. In this study, an in-house ELISA based on the construct of SARS-CoV-2 nucleocapsid (N) proteins, named rCoV2, rCoV4, and rCoV7, showed diagnostic performance for the detection of IgG antibodies. Sensitivity was evaluated in serum samples from patients with mild to moderate or severe COVID-19 infections, which were collected at different time points, while specificity was evaluated using pre-pandemic sera. In samples from mild to moderate cases obtained ≥16 days after the onset of symptoms, the sensitivities for rCoV2, rCoV4, and rCoV7 were 66.7%, 75%, and 77.8%, respectively. For samples from severe cases, the sensitivity was above 80% for all constructs. All proteins showed high specificity (94-98%). Overall, rCoV7 (C-terminus N-protein portion) showed better diagnostic performance, with 62.3% sensitivity in moderate and severe cases and 96.6% specificity. The SARS-CoV-2 ELISA using N-protein-based constructs could be a promisor tool for investigate the epidemiology of COVID-19 and monitor population-level serosurveillance. COVID19 COVID 19 COVID-1 pandemic tests SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- studies policies study inhouse house N (N rCoV2 rCoV rCoV4 antibodies points prepandemic pre sera 16 ≥1 symptoms 667 66 7 66.7% 75 75% 778 77 8 77.8% respectively 80 9498%. 9498 94 98% . 98 (94-98%) Overall Cterminus C terminus Nprotein protein portion 623 62 3 62.3 966 96 6 96.6 Nproteinbased populationlevel population level serosurveillance COVID1 1 COVID- SARS-CoV ≥ 66.7 77.8 9498% 949 9 (94-98% 62. 96. 66. 77. (94-98 (94-9 (94- (94 (9 (

RESUMEN Objetivos: reflexionar sobre el uso de referentes teóricos en la investigación en enfermería. Métodos: estudio teórico-reflexivo, basado en conceptos y constructos relevantes para el uso de las teorías de enfermería y otras ciencias, considerando cuestiones de epistemología o filosofía de la ciencia. Resultados: presentamos qué es y por qué hacer investigación en enfermería y qué es el marco teórico y por qué utilizarlo, además de algunas consideraciones sobre el uso de marcos teóricos en la investigación en enfermería, esencial para la construcción del conocimiento disciplinar, que posibilita la materialización del trabajo del investigador y la presentación de proposiciones resultantes de las investigaciones en y para la enfermería como disciplina y ciencia. Consideraciones Finales: a partir de una reflexión sustentada en concepciones epistemológicas, es posible afirmar que el marco teórico es el núcleo del pensamiento del investigador, al delimitar un problema a investigar y, con base en él, delinear las estrategias metodológicas a seguir, apoyando la acción y el pensamiento de la enfermería como disciplina y ciencia. Objetivos Métodos teóricoreflexivo, teóricoreflexivo reflexivo, reflexivo teórico-reflexivo ciencias ciencia Resultados utilizarlo disciplinar Finales epistemológicas él seguir

RESUMO Objetivos: refletir sobre o uso de referenciais teóricos nas pesquisas em enfermagem. Métodos: estudo teórico-reflexivo, fundamentado em conceitos e construtos pertinentes ao uso das teorias da enfermagem e outras ciências, considerando questões da epistemologia ou filosofia das ciências. Resultados: apresentamos o que é e por que fazer pesquisa em enfermagem, o que é o referencial teórico e por que utilizá-lo, além de ponderações concernentes ao uso de referenciais teóricos em enfermagem, essenciais para construção do conhecimento disciplinar, que possibilita a materialização do trabalho do pesquisador e a apresentação das proposições resultantes das investigações na e para a enfermagem enquanto ciência. Considerações Finais: a partir da reflexão pautada em concepções epistemológicas, é possível afirmar que o referencial teórico é o core do pensamento do pesquisador, ao delimitar um problema a ser investigado e, a partir dele, traçar estratégias metodológicas a serem seguidas, fundamentando o agir e pensar da enfermagem enquanto ciência. Objetivos Métodos teóricoreflexivo, teóricoreflexivo reflexivo, reflexivo teórico-reflexivo ciências Resultados utilizálo, utilizálo utilizá lo, lo utilizá-lo disciplinar ciência Finais epistemológicas dele seguidas

ABSTRACT Objectives: to reflect on theoretical framework use in nursing research. Methods: a theoretical-reflexive study, based on concepts and constructs pertinent to using nursing theories and other sciences, considering issues of epistemology or philosophy of science. Results: we presented what it is and why to do nursing research and what a theoretical framework is and why to use it, in addition to some considerations regarding theoretical framework use in nursing research, essential for constructing disciplinary knowledge, which enables the materialization of researchers’ work and the presentation of propositions resulting from investigations in and for nursing as a discipline and science. Final Considerations: based on a reflection based on epistemological conceptions, it is possible to affirm that a theoretical framework is the core of researchers’ thinking, delimiting a problem to be investigated and, based on it, outlining methodological strategies to be followed, supporting nursing action and thinking as discipline and science. Objectives Methods theoreticalreflexive reflexive study sciences science Results knowledge researchers Considerations conceptions followed

ABSTRACT Objective: To determine the prevalence of ocular alterations in patients diagnosed with dengue, admitted to a reference center for infectious and parasitic diseases in Manaus (AM), Brazil. Methods: This was an observational, analytical, and prospective case series study with 33 patients admitted to the hospital with positive clinical pathology tests during a 90-day period of a dengue epidemic in 2011. The investigations included measurement of visual acuity, macular assessment with Amsler grid test, fundus biomicroscopy, retinography and optical coherence tomography. Results: The average age of the patients was 35.48 years (minimum age 17 years and maximum 69 years). The main symptoms reported were blurred vision, followed by retro-ocular pain, photopsia, halos, foreign bodies, scotoma, double vision, and floaters. Visual acuity ranged from 20/20 to 20/40 (median 20/30). Macular assessment with Amsler Grid Test was unsatisfactory in three patients. The most common retinal findings were perimacular edema, macular edema, retinal hemorrhage, vasculitis, and changes in the retinal pigment epithelium. Optical coherence tomography showed retinal edema in 16 patients (51.51%). Conclusions: In all cases, the disease was self-limiting and resolved spontaneously without treatment. Therefore, this study confirms that ocular manifestations in dengue fever are varied and frequent. Objective AM, AM , (AM) Brazil Methods observational analytical 3 90day day 90 2011 test biomicroscopy Results 3548 35 48 35.4 minimum 1 6 years. . years) vision retroocular retro pain photopsia halos bodies scotoma floaters 2020 20 20/2 2040 40 20/4 median 20/30. 2030 20/30 30 20/30) hemorrhage vasculitis epithelium 51.51%. 5151 51.51% 51 (51.51%) Conclusions cases selflimiting self limiting treatment Therefore frequent (AM 9 201 354 4 35. 202 2 20/ 204 203 20/3 515 51.51 5 (51.51% 51.5 (51.51 51. (51.5 (51. (51 (5 (

RESUMO Objetivo: Determinar a prevalência de alterações oculares em pacientes com diagnóstico de dengue, internados em um centro de referência para doenças infecciosas e parasitárias em Manaus (AM), Brasil. Métodos: Estudo observacional, analítico e prospectivo de série de casos de 33 pacientes admitidos no hospital com exames de patologia clínica positivos durante um período de 90 dias de uma epidemia de dengue em 2011. As investigações incluíram medida da acuidade visual, avaliação macular com tela de Amsler, biomicroscopia de fundo de olho, retinografia e tomografia de coerência ótica. Resultados: A idade média dos pacientes foi de 35,48 anos (idade mínima de 17 anos e máxima de 69 anos). Os principais sintomas relatados foram visão turva, seguida de dor retro-ocular, fotopsia, halos, corpos estranhos, escotoma, visão dupla e moscas volantes. A acuidade visual variou entre 20/20 e 20/40 (mediana 20/30). A avaliação macular com a tela de Amsler foi insatisfatória em três doentes. Os achados retinianos mais comuns foram edema perimacular, edema macular, hemorragia retiniana, vasculite e alterações no epitélio pigmentar da retina. A tomografia de coerência óptica mostrou edema da retina em 16 doentes (51,51%). Conclusões: Em todos os casos, a doença foi autolimitada e resolveu-se espontaneamente sem tratamento. Este estudo confirma, portanto, que as manifestações oculares da dengue são variadas e frequentes. Objetivo AM, AM , (AM) Brasil Métodos observacional 3 9 2011 olho ótica Resultados 3548 35 48 35,4 1 6 anos. . anos) turva retroocular, retroocular retro ocular, ocular retro-ocular fotopsia halos estranhos escotoma volantes 2020 20 20/2 2040 40 20/4 mediana 20/30. 2030 20/30 30 20/30) perimacular retiniana 51,51%. 5151 51,51% 51 (51,51%) Conclusões resolveuse resolveu se tratamento confirma portanto frequentes (AM 201 354 4 35, 202 2 20/ 204 203 20/3 515 51,51 5 (51,51% 51,5 (51,51 51, (51,5 (51, (51 (5 (

ABSTRACT. The aim of this study was to evaluate the inclusion of different levels of soybean meal in pirarucu’s diet. 200 pirarucu juveniles (152.15 ± 0.8 g) were distributed in 20 polyethylene tanks with a continuous water flow system. A completely randomized experimental design was used, with five treatments (n = 4) containing 0, 10, 20, 30 and 40% of soybean meal inclusion levels. Growth performance was measured as specific growth rate, feed conversion, weight gain and survival. Body composition, protein retention rate and histopathological changes in the intestine were analyzed. The different treatments did not affect weight gain, specific growth rate and survival. The body composition of the fish was affected by treatments for ash and ether extract and the IHS was also affected. The diet with 40% soybean meal compromised feed conversion rate. Additionally, the distal intestine of fish fed with the same diet showed a reduction in the height of mucosal folds and a loss of supranuclear vacuolation in enterocytes. Based on these results, the inclusion of up to 30% of soybean meal in the diet of juvenile pirarucu is possible without negative effects on performance and enteric morphology. ABSTRACT pirarucus s 152.15 15215 152 15 (152.1 08 0 8 0. g 2 system used n 4 10 3 40 survival analyzed Additionally enterocytes results morphology 152.1 1521 1 (152. 152. (152 (15 (1 (

ABSTRACT Cowpea is one of the pulse crops that present the highest potential for growing in the Semiarid region of Brazil. Lowcost vegetable protein source, it has resilience to adverse conditions, however, in competition with weeds, its productivity can be strongly compromised. To overcoming this situation, it is essential for integrated management programs to determine the critical period for preventing interference. Thus, the objective of this work was to determine weed interference periods and the effect of coexistence periods on cowpea grain yield in the Semiarid region of the state of Minas Gerais, Brazil. The treatments were arranged in a 2×9 factorial scheme, as follows: cultivation in coexistence with weeds and cultivation under weed control, for nine crescent periods (0-7, 0-14, 0-21, 0-28, 0-35, 0-42, 0-49, 0-56 and 0-63 days after crop emergence), in a randomized experimental block design with four replications. The weed community was studied through phytosociological survey and evaluation of dry matter production and cowpea was evaluated for dry matter production, 100-grain mass and grain yield. The interference periods were determined using a non-linear logistic regression model. The weed community found was composed of 70% dicotyledonous and 30% monocotyledon plants, distributed in seven families and ten species. The coexistence with weeds results in losses of up to 90% in cowpea grain yield. The critical period of weed control in cowpea crop in the Semiarid region of the state of Minas Gerais, Brazil, is 11 to 36 days after crop emergence. Brazil source conditions however compromised situation Thus Gerais 29 2 9 2× scheme follows 07, 07 0 7, 7 (0-7 014, 014 14, 14 0-14 021, 021 21, 21 0-21 028, 028 28, 28 0-28 035, 035 35, 35 0-35 042, 042 42, 42 0-42 049, 049 49, 49 0-49 056 56 0-5 063 63 0-6 emergence, emergence , emergence) replications 100grain 100 nonlinear non linear model 70 30 plants species 90 1 3 (0- 01 0-1 02 0-2 03 0-3 04 4 0-4 05 5 0- 06 6 10 (0 (

RESUMO O feijão-caupi é uma das culturas de pulses com maior potencial de cultivo no semiárido brasileiro. Fonte de proteína vegetal de baixo custo, apresenta resiliência a condições adversas, entretanto, em competição com plantas daninhas, pode ter sua produtividade fortemente comprometida. Para superar essa situação, é indispensável aos programas de manejo integrado determinar o período crítico de prevenção à interferência. Assim, objetivou-se com este trabalho determinar os períodos de interferência de plantas daninhas e o efeito dos períodos de convivência sobre a produtividade do feijão-caupi no semiárido mineiro. Os tratamentos foram arranjados em esquema fatorial 2×9, da seguinte forma: cultivo em convivência com plantas daninhas e cultivo sob controle de plantas daninhas, por nove períodos crescentes (0-7, 0-14, 0-21, 0-28, 0-35, 0-42, 0-49, 0-56 e 0-63 dias após a emergência da cultura), em delineamento experimental de blocos casualizados com quatro repetições. A comunidade infestante foi estudada por meio de levantamento fitossociológico e avaliação da produção de matéria seca e o feijão-caupi foi avaliado quanto à produção de matéria seca, massa de 100 grãos e rendimento de grãos. Os períodos de interferência foram determinados adotando um modelo de equação não linear logístico. A comunidade infestante foi composta por 70% de plantas eudicotiledôneas e 30% de monocotiledôneas, distribuídas em sete famílias e dez espécies. A convivência com plantas daninhas resulta em até 90% de perdas na produtividade do feijão-caupi. O período crítico de controle de plantas daninhas no feijão-caupi no semiárido mineiro é de 11 a 36 dias após a emergência da cultura. feijãocaupi feijão caupi brasileiro custo adversas entretanto comprometida situação Assim objetivouse objetivou se 29 2 9 2×9 forma 07, 07 0 7, 7 (0-7 014, 014 14, 14 0-14 021, 021 21, 21 0-21 028, 028 28, 28 0-28 035, 035 35, 35 0-35 042, 042 42, 42 0-42 049, 049 49, 49 0-49 056 56 0-5 063 63 0-6 cultura, cultura , cultura) repetições 10 logístico 70 30 monocotiledôneas espécies 90 feijãocaupi. caupi. 1 3 2× (0- 01 0-1 02 0-2 03 0-3 04 4 0-4 05 5 0- 06 6 (0 (

The aim of this work was to identify and quantify the metabolites present in the roots of Combretum lanceolatum inoculated with its endophytic fungi. The metabolomics was accomplished using the 1H nuclear magnetic resonance (NMR) spectral data and evaluated via rNMR software and Madison Metabolomics Consortium Database (MMCD). The principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) showed that plants inoculated with Trichoderma spirale (Ts) present differentiation and discrimination over the time compared to control. Seven days after Ts fungal inoculation, 15 metabolites were identified at different concentrations comparing to the control plants. The plants inoculated with Ts fungus present the metabolites spermidine and pantothenate in higher concentrations and 3-hydroxybutyric acid and b-alanine in lower concentrations compared to control plants, indicating any response to biotic stress. These metabolites are involved in various plant processes, including secondary metabolites biosynthesis, energy metabolism and self-defense. Therefore, this work demonstrates the diversification of primary metabolites composition influenced by endophytes inoculation. fungi H NMR (NMR MMCD. MMCD . (MMCD) PCA (PCA squaresdiscriminant squares discriminant PLSDA PLS DA (PLS-DA (Ts inoculation 1 3hydroxybutyric hydroxybutyric 3 balanine b alanine stress processes biosynthesis selfdefense. selfdefense self defense. defense self-defense Therefore (MMCD

RESUMEN Objetivo: discutir el uso del software en el análisis de conceptos a través de: 1) consideraciones hechas por pares que aplican el programa en sus prácticas de enfermería; 2) correlación entre el uso del software NVivo 10® y las etapas de análisis de concepto utilizando el método Walker & Avant. Método: se trata de un ensayo teórico-reflexivo, basado en las etapas de análisis del concepto de Walker y Avant, construido después de un levantamiento bibliográfico y discusión con pares que aplican el marco metodológico y el software de análisis de datos en sus prácticas. Resultados: los resultados describen las etapas del proceso de elaboración y codificación del software de soporte a partir del análisis de concepto. Se presenta un cuadro con la demostración, descripción y comprensión de las etapas del referente metodológico en el uso del software auxiliar. Conclusión: el uso de un programa de análisis de conceptos puede facilitar la organización, visualización y acceso a los datos de la investigación. Aunque no reemplaza el trabajo del investigador y la relevancia del marco para el análisis, puede permitir la maximización de los resultados del proceso con beneficios para la investigación analítica. Objetivo 1 enfermería 2 10 Avant Método teóricoreflexivo, teóricoreflexivo teórico reflexivo, reflexivo teórico-reflexivo Resultados demostración auxiliar Conclusión organización analítica

ABSTRACT Objective: to discuss the use of software in concept analysis through: 1) considerations made by peers who apply the package in their nursing practices; 2) correlation between the use of the NVivo 10® software and the stages of concept analysis using the Walker & Avant method. Method: this is a theoretical-reflective essay, based on the stages of concept analysis by Walker and Avant, constructed after a bibliographic survey and discussion with peers who apply the methodological framework and data analysis software in their practices. Results: in the results, the stages of the preparation and coding process of the support software are described from the concept analysis. A table is presented with the demonstration, description and understanding of the stages of the methodological reference in the use of the auxiliary software. Conclusion: the use of a concept analysis program can facilitate the organization, visualization and access to research data. Although it does not replace the investigator's work and the relevance of the framework for analysis, it can allow for maximization of process results with benefits for analytical research. Objective through 1 practices 2 10 method Method theoreticalreflective theoretical reflective essay Results demonstration Conclusion organization investigators investigator s

RESUMO Objetivo: discutir sobre o uso de software na análise de conceito por meio de: 1) ponderações realizadas pelos pares que aplicam o programa em suas práticas na Enfermagem; 2) correlação do uso do software NVivo 10® com as etapas da análise de conceito pelo método de Walker & Avant. Método: trata-se de um ensaio teórico-reflexivo, fundamentado nas etapas da análise de conceito de Walker e Avant, construído após levantamento bibliográfico e discussão com pares que aplicam o referencial metodológico e software de análise de dados em suas práticas. Resultados: nos resultados, são descritas as etapas do processo de preparo e codificação do software de apoio a partir da análise de conceito. É apresentado um quadro com a demonstração, descrição e compreensão das etapas do referencial metodológico no uso do software de auxílio. Conclusão: o uso de programa na análise de conceito pode facilitar na organização, visualização e acesso aos dados da investigação. Embora não substitua o trabalho do investigador e a relevância do referencial para análise, pode permitir a maximização dos resultados do processo com benefícios para a pesquisa analítica. Objetivo 1 Enfermagem 2 10 Avant Método tratase trata se teóricoreflexivo, teóricoreflexivo teórico reflexivo, reflexivo teórico-reflexivo Resultados demonstração auxílio Conclusão organização investigação analítica

RESUMEN Objetivos: analizar la maduración conceptual de la Sistematización de los Cuidados de Enfermería en la perspectiva de la utilidad pragmática. Métodos: estudio de análisis de concepto. Los pasos fueron: seleccionar el concepto; elaborar preguntas analíticas; revisar exhaustivamente la literatura; y determinar componentes estructurales del concepto. Se analizaron 61 documentos luego de una búsqueda realizada hasta octubre de 2019. Resultados: ocurrieron cuatro períodos temporales de cambios contextuales desde el surgimiento de ideas de una sistematización en la década de 1960. Esta primera duró hasta 1990. Le siguieron las de 1990 a 2002, de 2002 a 2009 y de 2009 en adelante. Se identificó madurez conceptual parcial, operacionalización a lo largo de los años, a partir de múltiples definiciones, y, actualmente, un concepto de Sistematización de la Atención de Enfermería con atributos gerenciales y organizativo. Conclusiones: el concepto de Sistematización de la Atención de Enfermería está parcialmente maduro, tiene múltiples definiciones y se operativiza en conexiones inciertas con otros conceptos. Objetivos pragmática Métodos fueron analíticas literatura 6 2019 Resultados 1960 199 200 adelante parcial años actualmente organizativo Conclusiones maduro conceptos 201 196 19 20 1 2

ABSTRACT Objectives: to analyze the Systematization of Nursing Care conceptual maturation from the perspective of pragmatic utility. Methods: a concept analysis study. The stages were: select the concept; elaborate analytical questions; comprehensively review the literature; and determine concept structural components. Sixty-one documents were analyzed after a search carried out until October 2019. Results: four temporal periods of contextual changes have occurred since the emergence of ideas of a systematization in the 1960s. This first lasted until 1990. It was followed by those from 1990 to 2002, from 2002 to 2009 and from 2009 onwards. Partial conceptual maturity was identified, operationalization over the years, based on multiple definitions, and, currently, a concept of Systematization of Nursing Care with managerial and organizational attributes. Conclusions: the concept of Systematization of Nursing Care is partially mature, presents multiple definitions, being operationalized in uncertain connections with other concepts. Objectives utility Methods study questions literature components Sixtyone Sixty one 2019 Results 1960s s 199 200 onwards identified years definitions currently attributes Conclusions mature concepts 201 19 20 1 2

RESUMO Objetivos: analisar o amadurecimento conceitual de Sistematização da Assistência de Enfermagem na perspectiva da utilidade pragmática. Métodos: estudo de análise de conceito. As etapas foram: selecionar o conceito; elaborar questões analíticas; revisar a literatura de modo abrangente; e determinar componentes estruturais do conceito. Foram analisados 61 documentos após busca realizada até outubro de 2019. Resultados: quatro períodos temporais de mudanças contextuais ocorreram desde o surgimento das ideias de uma sistematização nos anos de 1960. Esse primeiro perdurou até 1990. Foi seguido pelos de 1990 a 2002, de 2002 a 2009 e a partir de 2009. Foi identificada parcial maturidade conceitual, operacionalização ao longo dos anos, com base em múltiplas definições, e, atualmente, um conceito de Sistematização da Assistência de Enfermagem com atributos gerenciais e organizativos. Conclusões: o conceito de Sistematização da Assistência de Enfermagem é parcialmente maduro, apresenta múltiplas definições, sendo operacionalizado em ligações incertas com outros conceitos. Objetivos pragmática Métodos foram analíticas abrangente 6 2019 Resultados 1960 199 200 definições atualmente organizativos Conclusões maduro conceitos 201 196 19 20 1 2

Food additives are chemical substances added to improve organoleptic characteristics, in this context, there are several synthetic dyes, such as sunset yellow, tartrazine, erioglaucine, allura red, indigo carmine, and new coccine, that can also be identified through different analytical techniques such as infrared spectroscopy, UV-Vis spectrophotometry, high performance liquid chromatography with diode array detector and mass spectrometry. Therefore, the objective of this work was to compare methods developed for different analytical techniques, in order to identify the one that presents greater efficiency in certain applications. For this, the patterns of the dyes were analyzed, as well as their mixtures and foods that had them. As a result, the CLAE-DAD presents excellent selectivity and linearity of the calibration curves, but it presents higher LD and LQ values when compared to the UV-Vis, in turn the infrared provides information regarding functional groups and bonds. Mass spectrometry showed great capabilities in identifying the molecular formula and better selectivity in the absence of the erioglaucine dye. It is not feasible to indicate the best or worst analytical approach, but it is possible to point out which one is more suitable for each application. Regarding the quantification capacity, chromatography and UV-Vis are good alternatives, but in mixtures containing more than one dye, CLAE-DAD is more suitable due to the better selectivity. Regarding the identification capacity, mass spectrometry, especially FT-ICR MS, is the most appropriate technique to propose molecular formulas due to its high resolution and mass accuracy. The advantage of FTIR was the easier sample preparation, being able to analyze the dyes in the solid state. characteristics context yellow tartrazine red carmine coccine spectroscopy UVVis UV Vis spectrophotometry Therefore applications analyzed them result CLAEDAD CLAE DAD curves UVVis, Vis, bonds dye approach application capacity alternatives FTICR FT ICR MS accuracy preparation state

ABSTRACT Background: Women living with human immunodeficiency virus (HIV) (WLWH) are more likely to be infected with the oncogenic human papillomavirus (HPV). We assessed the prevalence of high-risk (HR) (16/18/31/33/35/39/45/51/52/56/58/59/68/73/82), probable high-risk (pHR) (26/53/66), and low-risk (LR) (6/11/40/42/43/44/54/61/70) HPV types and their associated risk factors. Methods: This cross-sectional study of WLWH aged 18-64 years included one laboratory and eight HIV-specialty healthcare facilities in the pilot network. Descriptive statistics were used to assess sociodemographic and behavioral characteristics. Adjusted analyses were conducted to evaluate risk factors associated with HR and/or pHR HPV infection in WLWH. Results: From May/2021 to May/2022, 1,914 (92.5%) WLWH participated in the pilot study and had valid HPV-DNA results of self-collected vaginal samples. The median age of the participants was 45 years, 60.1% had ≥ 9 years of schooling, 80.5% were ≤ 18 years at first sexual intercourse, and 51.7% had > 4 sexual partners throughout life. The prevalence of any HPV type, HR HPV, pHR HPV, and LR HPV was 65.8%, 49.6%, 16.7%, and 40.0%, respectively. Age was inversely associated with pHR and/or HR-HPV (p < 0.001), and education level was inversely associated with HR-HPV (p = 0.003) types. Any HR or pHR was associated with being single (p = 0.029) and exchanging sex for drugs (p = 0.037). Conclusions: The prevalence of HPV, especially HR HPV, among WLWH is high in Brazil, highlighting the need for HPV screening in this population. Self-collection of vaginal samples is an important strategy for increasing testing access. Background HIV (HIV (WLWH HPV. . (HPV) highrisk (HR 16/18/31/33/35/39/45/51/52/56/58/59/68/73/82, 161831333539455152565859687382 16/18/31/33/35/39/45/51/52/56/58/59/68/73/82 , 16 31 33 35 39 51 52 56 58 59 68 73 82 (16/18/31/33/35/39/45/51/52/56/58/59/68/73/82) (pHR 26/53/66, 265366 26/53/66 26 53 66 (26/53/66) lowrisk low (LR 6/11/40/42/43/44/54/61/70 61140424344546170 6 11 40 42 43 44 54 61 70 (6/11/40/42/43/44/54/61/70 Methods crosssectional cross sectional 1864 64 18-6 HIVspecialty specialty network characteristics andor Results May2021 May 2021 May/202 May2022 2022 May/2022 1914 1 914 1,91 92.5% 925 92 5 (92.5% HPVDNA DNA selfcollected self collected 601 60 60.1 schooling 805 80 80.5 intercourse 517 7 51.7 life type 658 65 8 65.8% 496 49 49.6% 167 16.7% 400 0 40.0% respectively HRHPV p 0.001, 0001 0.001 001 0.001) 0.003 0003 003 0.029 0029 029 0.037. 0037 0.037 037 0.037) Conclusions Brazil population Selfcollection Self collection access (HPV 16183133353945515256585968738 16/18/31/33/35/39/45/51/52/56/58/59/68/73/8 3 (16/18/31/33/35/39/45/51/52/56/58/59/68/73/82 26536 26/53/6 2 (26/53/66 6/11/40/42/43/44/54/61/7 6114042434454617 (6/11/40/42/43/44/54/61/7 186 18- May202 202 May/20 191 91 1,9 92.5 (92.5 60. 80. 51. 65.8 49.6 16.7 40.0 000 0.00 00 0.02 002 02 0.03 03 1618313335394551525658596873 16/18/31/33/35/39/45/51/52/56/58/59/68/73/ (16/18/31/33/35/39/45/51/52/56/58/59/68/73/8 2653 26/53/ (26/53/6 6/11/40/42/43/44/54/61/ 611404243445461 (6/11/40/42/43/44/54/61/ May20 20 May/2 19 1, 92. 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Abstract The East Brazilian continental margin contains a large shelf sector called the Royal Charlotte Bank. It has terrigenous and carbonate sedimentation associated with coastal reefs. Studies using X-ray diffractometry (XRD) and the geochemistry of immobile elements were done in samples of these reefs to figure out the provenance of the mud that arrives there. In order to do this, samples of riverine sediment from the Jequitinhonha, Santo Antônio, João de Tiba, and Buranhém rivers as well as from the Barreiras Group’s sedimentary cliffs were taken. The Jequitinhonha River was found to be the most significant mud source. This was supported by the mineral fingerprints of smectite and biotite, the concentrations of immobile trace elements such as Zr, Hf, Nb, Ta, and Th, and the distribution and ratio of rare-earth elements. These findings are supported by the prevailing north-to-south drift that occurs in the region due to the northeasterly trade winds and waves that blow throughout the region for the vast majority of the year. The findings have significant implications for hydrographic basin management and the protection of reef benthic populations. Bank Xray X ray XRD (XRD there this Antônio Tiba Groups Group s taken source biotite Zr Hf Nb Ta Th rareearth rare earth northtosouth north south year populations

Abstract This work was carried out in order to evaluate the initial growth in different conditions of shadings of the species Copernicia alba, Copernicia hospita and Copernicia macroglossa. Initially the seed were soaked in water to a pre-germination, afterward were sowed in tubes (288 ml) filled with substrate (sandy loam + organic compost + vermiculite) in proportion 2:1:1 and kept in full sun, 30%, 50% e 70% shading. It was adopted the completely randomized design, with five repetitions (4 plants/repetition) and five periods of evaluation: 45, 90, 135, 180 and 225 days after sowing, with the factors disposed in a spli-splot measured over time (4 shading levels x 5 evaluation times). Each period of evaluation were measured the following variables: number of leaves, height, stem diameter, foliar area, dry mass of aerial parts and roots, Dickson of quality index. The seedling of Copernicia cultivated in full sun and 30% shading express positive effects in the initial growth with good quality. The largest leaf areas of the species were found in the environment with 30% shading. In face of these results, the obtained data are important and indispensable to establishment of seedling production of quality of Copernicia. alba macroglossa pregermination, pregermination pre germination, germination pre-germination 288 (28 ml sandy vermiculite 211 2 1 2:1: 30 50 70 design 4 ( plants/repetition plantsrepetition plants repetition 45 90 135 18 22 sowing splisplot spli splot times. times . times) variables leaves height diameter area roots index results 28 (2 21 2:1 3 7 9 13 2:

Resumo Este trabalho foi desenvolvido com o objetivo de avaliar o crescimento inicial em diferentes condições de sombreamento das espécies Copernicia alba, Copernicia hospita e Copernicia macroglossa. Inicialmente, as sementes foram embebidas em água para uma pré-germinação, em seguida foram semeadas em tubetes (288 ml) preenchidos com substrato (arisco + composto orgânico + vermiculita) na proporção de 2:1:1 e mantidas a pleno sol, 30%, 50% e 70% de sombreamento. Adotou-se o delineamento inteiramente casualizado com 5 repetições (4 plantas/repetição) e cinco períodos de avaliação 45, 90, 135, 180 e 225 dias após a semeadura, com fatores dispostos em arranjo de parcelas subdivididas no tempo (4 níveis de sombreamento x 5 épocas de avaliação). A cada época de avaliação, foram mensuradas as seguintes variáveis respostas: número de folhas, altura, diâmetro do coleto, área foliar, massa seca das partes aéreas e das raízes e índice de qualidade de Dickson. As mudas de Copernicia cultivadas em pleno sol e 30% de sombreamento expressam efeitos positivos no crescimento inicial com boa qualidade. Os melhores resultados de área foliar das espécies foram encontrados no ambiente com 30% de sombreamento. Diante desses resultados, os dados obtidos são importantes e indispensáveis ao estabelecimento de produção de mudas de qualidade de Copernicia. alba macroglossa Inicialmente prégerminação, prégerminação pré germinação, germinação pré-germinação 288 (28 ml arisco vermiculita 211 2 1 2:1: 30 50 70 Adotouse Adotou se 4 ( plantas/repetição plantasrepetição plantas repetição 45 90 135 18 22 semeadura avaliação. . avaliação) respostas folhas altura coleto Dickson 28 (2 21 2:1 3 7 9 13 2:

RESUMO A salinidade da água de irrigação ou do solo são fatores abióticos que mais impacta negativamente na produtividade das culturas, entre elas, o milho verde, sendo necessário encontrar alternativas para garantir a produção. Nesse contexto, objetivou-se avaliar as trocas gasosas, os pigmentos fotossintéticos e o crescimento do milho sob salinidade da água de irrigação e aplicação de peróxido de hidrogênio. O experimento foi conduzido no Centro de Ciências e Tecnologia Agroalimentar, Pombal, PB, pertencente à Universidade Federal de Campina Grande, no período de janeiro a fevereiro de 2015, utilizando-se do híbrido de milho ‘AG 1051’. Os tratamentos foram constituídos por dois níveis de salinidade da água de irrigação (0,3 e 2,0 dS m-1) e cinco concentrações de peróxido de hidrogênio (0, 40, 80, 160 e 320 µmol L-1) aplicadas via água de irrigação. O delineamento experimental foi o inteiramente casualizado, no esquema fatorial 2 × 5, com quatro repetições. A CEa de 2,0 dS m-1 reduz a transpiração, condutância estomática, clorofila total, carotenóides e crescimento inicial, mas não afeta o acúmulo de massa seca das plantas de milho. A aplicação de H2O2 via solo variando de 0 a 320 µmol L-1 promoveu redução na taxa de assimilação de CO2 e transpiração, bem como em concentrações de 0 a 160 µmol L-1 para condutância estomática de plantas irrigadas com CEa de 2,0 dS m-1. Aplicação de H2O2 via solo até a concentração de 320 µmol L-1 aumentou a altura de plantas, porém reduziu o diâmetro do colmo de milho.

ABSTRACT The salinity of irrigation water or soil is the abiotic factor that most negatively impacts the yield of crops, including green maize, so it is necessary to find alternatives to ensure production. In this context, this study aimed to evaluate gas exchange, photosynthetic pigments, and growth of maize under salinity of irrigation water and application of hydrogen peroxide. The experiment was conducted at the Center of Sciences and Agrifood Technology, Pombal, PB, belonging to the Federal University of Campina Grande, in the period from January to February 2015, using the maize hybrid ‘AG 1051’. The treatments consisted of two salinity levels of irrigation water (0.3 and 2.0 dS m-1) and five concentrations of hydrogen peroxide (0, 40, 80, 160, and 320 µmol L-1) applied via irrigation water. The experimental design was completely randomized, in a 2 × 5 factorial scheme, with four replicates. ECw of 2.0 dS m-1 reduces transpiration, stomatal conductance, total chlorophyll, carotenoids, and initial growth, but does not affect the dry mass accumulation of maize plants. Application of H2O2 via soil varying from 0 to 320 µmol L-1 causes reductions in the CO2 assimilation rate and transpiration, as well as at concentrations from 0 to 160 µmol L-1 for stomatal conductance of plants irrigated with ECw of 2.0 dS m-1. Application of H2O2 via soil up to a concentration of 320 µmol L-1 increases the plant height, but reduces culm diameter of maize.

ABSTRACT The objective of this study was to determine the effects that breeder age has on digestive and immune system development; the transfer of immunoglobulins to egg yolk, yolk sac, and neonate chicks; and the immune response of chicks up to 35 days old. Three ages (32, 42, and 52 weeks) of Hubbard breeders were studied with ages as treatments. A total of 425 eggs were weighed for each of the three treatments and incubated. After hatching, a total of 300 1-day-old chicks were used in each treatment. We studied the development of the gastrointestinal tract and immune system of progeny and IgY transfer from breeder to progeny. Chicks from 52-week-old breeders had greater gastrointestinal tract growth up to seven days of life and greater body weight at 14 days. Older breeders (52 weeks) had higher amounts of IgY in serum and egg yolk. Chicks from the youngest breeders (32-weeks-old) had a better immune response at two weeks post-vaccination. It can be concluded that the older breeders have a greater capacity to immunize progeny up to 14 days. Strategies can be developed to increase IgY in the serum of young breeders and, consequently, increase the innate immunity of the newly-hatched chicks.

RESUMO Objetivo: Identificar se há mudança refracional significativa após realização de capsulotomia posterior com laser Nd:YAG em olhos pseudofácicos. Métodos: Estudo retrospectivo com análise de prontuários de pacientes atendidos em um hospital com diagnóstico de opacificação de cápsula posterior do cristalino tratada com capsulotomia posterior com laser Nd:YAG no período de outubro de 2019 a março de 2021. A comparação entre a refração antes e após o procedimento foi realizada calculando-se o equivalente esférico. Também foi avaliada a mudança da acuidade visual, aferida por LogMAR. Resultados: Foram analisados 90 prontuários, totalizando 140 olhos, de pacientes submetidos à capsulotomia posterior com laser Nd:YAG. O equivalente esférico médio pré-procedimento foi de -0,07±0,89D, mínimo de -3,0D e máximo de +2,5D, mediana (intervalo interquartil) de 0,0D (-0,50D a +0,375D). A média pós-procedimento foi de -0,18±0,86D, mínimo de -3,5D e máximo de +2,25D, mediana (intervalo interquartil) de -0,125D (-0,50D a 0,0D). com p<0,0082. Dos 140 olhos, 66 sofreram miopização e 37 hipermetropização. A média de alteração do equivalente esférico geral foi de -0,12±0,51D, mínimo de -2,50D e máximo de +1,25D, mediana (intervalo interquartil) de 0,0D (-0,375D a +0,125D). Ao se comparar a diferença entre o equivalente esférico antes e após o procedimento do grupo de olhos que sofreu miopização (n=66) ou hipermetropização (n=37), separadamente, ambos obtiveram p<0,0001. Ao se compararem todos os olhos que sofreram alguma alteração refracional (n=103), foi encontrado p=0,008. A acuidade visual média pré-procedimento foi de 0,23±0,32, mínimo de 0,0 e máximo de 2,3. Pós-procedimento, a média foi de 0,06±0,13, mínimo de -0,12 e máximo de 0,7, com p<0,0001. Conclusão: A capsulotomia posterior com laser Nd:YAG gerou melhora significativa da acuidade visual nos pacientes do estudo, porém também gerou alteração refracional significativa após o procedimento, tanto para miopização (a mais frequente), quanto para hipermetropização.

ABSTRACT Objective: To identify if there is a significant change on refraction after Nd:YAG laser posterior capsulotomy in pseudophakic eyes. Methods: A retrospective study with analysis of medical records of patients treated at a hospital, with diagnosis of opacification of posterior lens capsule treated with Nd:YAG laser posterior capsulotomy, from October 2019 to March 2021. The comparison of refraction before and after the procedure was performed by calculating the spherical equivalent. Changes in visual acuity (VA), measured by LogMAR, were also evaluated. Results: A total of 90 medical records (140 eyes) of patients submitted to Nd:YAG laser posterior capsulotomy were analysed. The mean pre-procedure spherical equivalent was -0.07±0.89D, minimum of -3.0D and maximum of +2.5D, median (interquartile range) of 0.0D (-0.50D to +0.375D). The post-procedure mean was -0.18±0.86D, minimum of -3.5D and maximum of +2.25D, median (interquartile range) of -0.125D (-0.50D to 0.0D), with p <0.0082. Of the 140 eyes, 66 underwent myopia and 37 hyperopia, the mean change in the general spherical equivalent was -0.12±0.51D, minimum -2.50D and maximum +1.25D, median (interquartile range) of 0.0D (-0.375D to +0.125D). When comparing the difference between the spherical equivalent before and after the procedure of the group of eyes that underwent myopia (n=66) or hyperopia (n=37), separately, both obtained p<0.0001. When comparing all eyes that suffered any change on refraction (n=103), the p value was 0.008. The mean pre-procedure visual acuity was 0.23±0.32, minimum of 0.0 and maximum of 2.3. After the procedure, the mean was 0.06±0.13, minimum of -0.12 and maximum of 0.7, p<0.0001. Conclusion: Nd:YAG laser posterior capsulotomy significantly improved visual acuity of patients in this study; however, it also led to a significant change on refraction after the procedure, both for myopization, which was more frequent, and for hyperopization.