Abstract Healthcare workers present an increased risk of contagion for the SARS-CoV-2 virus due to their labor exposure. Here, we describe the clinical, laboratory, and immunological characteristics of healthcare workers, before vaccine application, exposed to SARS-CoV-2-infected patients. We collected sociodemographic, clinical, and laboratory information from 50 professionals who worked during the COVID-19 pandemic at the Clinical Hospital of the Northwest in Brazil. The results showed that most workers are women, over 50 years old, and worked as nursing technicians. Approximately 56% of workers were positive for a previous infection by RT-PCR and/or anti-SARS-CoV-2-immunoglobulin tests. Increased levels of hematocrit, neutrophils, NK lymphocytes, and fibrinogen, were found in positive healthcare workers, suggesting a light inflammatory status. The immunological findings showed an increase in IL-17 production and a Th2/Th17/Th22 profile followed by high serology for anti-SARS-CoV-2 IgM and IgG. Those data reveal the importance of studies with healthcare workers to investigate if the continuous exposition to the virus may result in chronic activation of the immune system and/or pulmonary inflammation in this target group. SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- exposure Here clinical application SARSCoV2infected SARSCoVinfected infected patients sociodemographic 5 COVID19 COVID 19 COVID-1 Brazil women old technicians 56 RTPCR RT PCR andor or antiSARSCoV2immunoglobulin antiSARSCoVimmunoglobulin anti immunoglobulin tests hematocrit neutrophils lymphocytes fibrinogen status IL17 IL 17 IL-1 Th2Th17Th22 ThThTh Th2 Th17 Th22 Th Th2/Th17/Th2 antiSARSCoV2 antiSARSCoV anti-SARS-CoV- IgG group SARS-CoV COVID1 1 COVID- IL1 IL- Th2Th17Th2 Th1 Th2/Th17/Th anti-SARS-CoV Th2Th17Th

Resumen Nasutitermes corniger es una termita arbórea que causa daños económicos en las zonas urbanas, y su control es mediante insecticidas químicos. Una alternativa para el control de insectos es el uso de hongos entomopatógenos y extractos de plantas, o el efecto sinérgico de la asociación de estos. Se examinó la toxicidad de los extractos acuosos e hidroetanólicos de Agave sisalana sobre los aspectos biológicos de los hongos Cordyceps farinosa, C. fumosorosea y C. javanica y la acción de los extractos y su efecto sinérgico sobre N. corniger. Se evaluó el efecto de los extractos sobre hongos por germinación, esporulación y crecimiento micelial de los hongos en medio Sabouraud mediante el estudio de aspectos biológicos bajo condiciones de laboratorio y se examinó el control de N. corniger, por ingestión de extractos y de la asociación extracto y hongo sobre papel de filtro, siendo el porcentaje de mortalidad de soldados y obreras de la termita diaria. En general, los extractos resultaron inofensivos para los hongos a concentraciones de 10, 25, 50, 100 y 200 mg.mL-1. Los extractos demostraron acciones termiticidas a las concentraciones ensayadas, provocando la mortalidad de alrededor del 100% de los trabajadores después del tercer y sexto día (LC50: 0.778 a 0.803 mg.mL-1) y del 100% de los soldados entre el cuarto y séptimo días (LC50: 0.146 a 0.956 mg.mL-1). La asociación de los extractos con C. farinosa ESALQ1355 fue más eficiente para inducir la mortalidad en N. corniger. Estos resultados demostraron la eficiencia in vitro de los extractos de sisal en el control de las termitas de N. corniger, solas o en combinación con hongos, lo que sugiere su potencial utilidad conjunta en el control biológico de plagas de insectos.

Abstract Nasutitermes corniger is an arboreal termite that causes economic damage in urban areas, and its control is for chemical insecticides. An alternative for insect control is the use of entomopathogenic fungi and plant extracts, or the synergistic effect of the association of these. The toxicity of aqueous and hydroethanolic extracts of Agave sisalana on the biological aspects of the fungal Cordyceps farinosa, C. fumosorosea and C. javanica and the action of the extracts and their synergistic effect on N. corniger was examined. The effect of the extracts on fungi was evaluated by germination, sporulation and mycelial growth of fungi in Sabouraud medium through the study of biological aspects under laboratory conditions; and control of N. corniger were examined, by ingestion of extracts and of the association extract plus fungus on filter paper, being the percentage of mortality of soldiers and workers of the termite daily. In general, the extracts were inoffensive to the fungi at concentrations 10, 25, 50, 100 and 200 mg.mL-1. The extracts demonstrated termiticidal actions at concentrations tested, causing the mortalities about 100% of the workers after the third and sixth days (LC50: 0.778 to 0.803 mg.mL-1) and 100% of the soldiers between the fourth and seventh days (LC50:0.146 to 0.956 mg.mL-1). The association of the extracts with C. farinosa ESALQ1355 was more efficient in inducing the mortality in N. corniger. These results demonstrated the efficiency in vitro of the sisal extracts in controlling N. corniger termites, alone or in combination with fungi, suggesting their potential joint usefulness in the biological control of insect pests.

RESUMO Objetivo: Este estudo teve como objetivo investigar o relacionamento entre as crenças religiosas e os constructos da Teoria do Comportamento Planejado na intenção empreendedora. Originalidade/valor: O artigo se destaca por fomentar a compreensão do empreendedorismo a partir da Teoria do Comportamento Planejado, proposta por Ajzen (1991), alinhando-a com as crenças religiosas. Design/metodologia/abordagem: Trata-se de um estudo exploratório e descritivo de natureza quantitativa. Adotou-se o método survey para a coleta dos dados, em que foi obtida uma amostra de 448 estudantes do curso de Administração de duas universidades públicas do Nordeste. Utilizou-se a Modelagem de Equações Estruturais para o tratamento e a análise dos dados. Resultados: Os resultados apontaram que a atitude pessoal, o controle percebido e as crenças religiosas exerceram influência sobre a intenção empreendedora de estudantes universitários, o que não ocorreu com as normas subjetivas. Contudo, as normas subjetivas influenciaram a atitude pessoal e o controle percebido dos estudantes universitários. Os universitários apresentaram uma predisposição ao empreendedorismo e reconheceram que têm as competências necessárias para criação de negócios. Contudo, a escolha em se tornar empreendedor pode não ser motivada, diretamente, pela expectativa de seus referentes sociais. Ademais, os universitários não perceberam a influência das crenças religiosas na manifestação do seu comportamento, pelo fato de a vocação e a motivação pró-social estarem distantes do contexto ambiental em que os universitários estão inseridos ou por não serem praticantes de uma religião que fomente tais crenças.

ABSTRACT Purpose: To investigate the relationship between religious beliefs and the constructs of the Theory of Planned Behavior in entrepreneurial intention. Originality/value: The article stands out by promoting the understanding of entrepreneurship from the Theory of Planned Behavior proposed by Ajzen (1991), aligning it with religious beliefs. Design/methodology/approach: It is an exploratory and descriptive study, of a quantitative nature. The survey method was used to collect the data, in which a sample of 448 students from the Business Administration courses at two public universities in the northeast of Brazil was obtained. Structural Equation Modeling was used to treat and analyze the data. Findings: The results demonstrated that personal attitude, perceived control, and religious beliefs influence the entrepreneurial intention of university students, which did not occur with subjective norms. However, subjective norms influenced the personal attitude and perceived control of university students. The university students presented a predisposition to entrepreneurship and recognized that they have the necessary skills to create a business. However, the choice to become an entre preneur may not be motivated directly by the expectation of its social referents. Moreover, university students did not perceive the influence of religious beliefs in the manifestation of their behavior, because vocation and prosocial motivation are far from the environmental context in which university students are inserted or because they are not practicing a religion that fosters such beliefs.

Abstract: Currently, the research of new natural compounds with biological potential demonstrates great ethnopharmacological importance. In this study, we evaluated the biological properties promoted by saline extract from Malpighia emarginata DC leaves, whose objective is to evaluate the antioxidant, antimicrobial and cytotoxicity potential. Phytochemical characterization was performed by UPLC-MS chromatography to identify the chemical compounds. For the antioxidant potential, DPPH, ATT and FRAP methods were used. The antibacterial and antifungal tests were performed evaluating the MIC50, MIC90, CMB and CMF parameters. Moreover, antibiofilm action was evaluated. Cytotoxicity and proliferation were performed using splenocytes from Balb/c mice and were evaluated by cytometry. We found a list of phenolic compounds among other bioactive compounds in the M. emarginata saline extract. In addition, higher antioxidant profile and antifungal activity against different strains of Candida spp. was promoted by the saline extract. Splenocytes showed greater cell viability (more than 90%) and showed higher proliferate index in 24 and 48 hours of incubation with the extract. Saline extract from Malpighia emarginata DC has potential action like antioxidant and antifungal agent without promote animal cell damage.

Abstract: We evaluated the antifungal and antibiofilm potential of the hydroalcoholic extract of bark from Anadenanthera colubrina (vell.) Brenan, known as Angico, against Candida spp. Antifungal activity was evaluated using the microdilution technique through the Minimum Inhibitory and Fungicide Concentrations (MIC and MFC). The antibiofilm potential was tested in mature biofilms formed by Candida species and analyzed through the counting of CFU/mL and scanning electron micrograph (SEM). In vivo toxicity and therapeutic action was evaluated in the Galleria mellonella model. The treatment with the extract, in low doses, was able to reduce the growth of planktonic cells of Candida species. MIC values range between 19.5 and 39 µg/mL and MFC values range between 79 and 625 µg/mL. In addition was able to reduce the number of CFU/mL in biofilms and to cause structural alteration and cellular destruction, observed via SEM. A. colubrina showed low toxicity in the in vivo assay, having not affected the viability of the larvae at doses below 100mg/kg and high potential in the treatment of C. albicans infection. Considering its high antifungal potential, its low toxicity and potential to treatment of infections in in vivo model, A. colubrina extract is a strong candidate for development of a new agent for the treatment of oral candidiasis.

Pesquisa com a finalidade de compreender os limites/possibilidades de implementação de ações de saúde mental nos serviços da rede básica de saúde do município de Cabedelo PB, na perspectiva da Reforma Psiquiátrica proposta no país. Tem referencial teórico-metodológico no Materialismo Histórico e Dialético. O material empírico foi analisado pela técnica de análise do discurso. Os resultados indicaram que o processo de trabalho dos enfermeiros identifica-se com o processo de trabalho Assistir (Cuidar/Fazer). Preserva aspectos do modelo tradicional, mas, incorpora outros enfoques da Saúde Coletiva, num movimento que requer a compreensão dos pressupostos do movimento pela Reforma Psiquiátrica para a transformação do processo de trabalho de enfermagem, no sentido da inclusão social e da integralidade da assistência.

Research with the purpose of understanding the limits/possibilities of the implementation of mental health actions in the services of the basic health system of the municipal district of CabedeloPB, in the perspective of the Psychiatric Reform proposed in the country. It has theoretical-methodological reference in the Historical Materialism and Dialectics. The empiric material was analyzed by the technique of speech analysis. The results indicated that the work process of the nurses identifies with the work process to Attend (Care/Making). It preserves aspects of the traditional model, but it incorporates other focuses of the Collective Health, in a movement that requests the understanding of the presuppositions of the movement for the Psychiatric Reform for the transformation of the work process of nursing, in the sense of the social inclusion and of the integrality of the assistance.

Investigación con la finalidad de comprender los limites/posibilidades de implementación de acciones de salud mental en los servicios de la rede básica de salud del municipio de Cabedelo PB, en la perspectiva de la Reforma Psiquiátrica propuesta en el país. Tiene referencial teórico-metodológico lo Materialismo Histórico e Dialéctico. El material empírico fue analizado por la técnica de análisis del discurso. Los resultados indicaran que el proceso de trabajo de los enfermeros identifica-se con el proceso de trabajo Asistir (Cuidar/Fazer). Preserva aspectos del modelo tradicional, pero, incorpora otros enfoques de la Salud Colectiva, en un movimiento que requiere la comprensión de los presupuestos del movimiento por la Reforma Psiquiátrica para la transformación del proceso de trabajo de enfermería, en el sentido de la inclusión social e de la integralidad de la asistencia.

Research with the purpose of understanding the limits/possibilities of the implementation of mental health actions in the services of the basic health system of the municipal district of CabedeloPB, in the perspective of the Psychiatric Reform proposed in the country. It has theoretical-methodological reference in the Historical Materialism and Dialectics. The empiric material was analyzed by the technique of speech analysis. The results indicated that the work process of the nurses identifies with the work process to Attend (Care/Making). It preserves aspects of the traditional model, but it incorporates other focuses of the Collective Health, in a movement that requests the understanding of the presuppositions of the movement for the Psychiatric Reform for the transformation of the work process of nursing, in the sense of the social inclusion and of the integrality of the assistance.

Investigación con la finalidad de comprender los limites/posibilidades de implementación de acciones de salud mental en los servicios de la rede básica de salud del municipio de Cabedelo PB, en la perspectiva de la Reforma Psiquiátrica propuesta en el país. Tiene referencial teórico-metodológico lo Materialismo Histórico e Dialéctico. El material empírico fue analizado por la técnica de análisis del discurso. Los resultados indicaran que el proceso de trabajo de los enfermeros identifica-se con el proceso de trabajo Asistir (Cuidar/Fazer). Preserva aspectos del modelo tradicional, pero, incorpora otros enfoques de la Salud Colectiva, en un movimiento que requiere la comprensión de los presupuestos del movimiento por la Reforma Psiquiátrica para la transformación del proceso de trabajo de enfermería, en el sentido de la inclusión social e de la integralidad de la asistencia.

Pesquisa com a finalidade de compreender os limites/possibilidades de implementação de ações de saúde mental nos serviços da rede básica de saúde do município de Cabedelo PB, na perspectiva da Reforma Psiquiátrica proposta no país. Tem referencial teórico-metodológico no Materialismo Histórico e Dialético. O material empírico foi analisado pela técnica de análise do discurso. Os resultados indicaram que o processo de trabalho dos enfermeiros identifica-se com o processo de trabalho Assistir (Cuidar/Fazer). Preserva aspectos do modelo tradicional, mas, incorpora outros enfoques da Saúde Coletiva, num movimento que requer a compreensão dos pressupostos do movimento pela Reforma Psiquiátrica para a transformação do processo de trabalho de enfermagem, no sentido da inclusão social e da integralidade da assistência.

A doença de Huntington (HD) é afecção neurodegenerativa com padrão de herança autossômica dominante caracterizada por movimentos involuntários coreiformes e alterações cognitivas. O início dos sintomas ocorre em torno dos 40 de idade, progredindo até a morte em um período de aproximadamente 10 a 15 anos após o início da doença. A HD está associada a uma expansão trinucleotídica CAG presente na porção 5' do gene IT15. Nós investigamos a repetição CAG no gene IT15 em 44 indivíduos brasileiros (42 pacientes e 2 membros não afetados de uma família) pertencentes a 34 famílias não relacionadas com diagnóstico provável de HD. Um alelo CAG expandido foi encontrado em 32 indivíduos (76%) pertencentes a 25 famílias não relacionadas. Nos pacientes com a mutação HD, os alelos expandidos variaram de 43 a 73 unidades de repetição CAG e os alelos normais apresentaram variação de 18 a 26 CAGs. Correlação negativa significativa foi encontrada entre a idade de início dos sintomas e o tamanho da expansão trinucleotídica CAG (r=0,6; p=0,0001); no entanto, o tamanho da repetição CAG expandida foi capaz de explicar somente 40% da variação encontrada na idade de início da doença (r2=0,4). Além disso, nós genotipamos um total de 25 indivíduos pertencentes a um grupo controle da população brasileira (total de 50 alelos), sendo que o alelo normal apresentou variação de 16 a 33 unidades de repetição CAG. A porcentagem de heterozigozidade do alelo normal na população brasileira controle foi 88%. Em conclusão, nossos resultados mostraram que nem todos os pacientes com fenótipo ``HD'' possuíam a expansão CAG no gene IT15. O diagnóstico molecular foi possível em todos os indivíduos analisados nesse estudo, não sendo encontrada em nossa amostra nenhum alelo de tamanho intermediário. Portanto, nós recomendamos que a confirmação molecular do diagnóstico na HD deva ser realizada em todos os casos suspeitos, a fim de proporcionar subsídios para um aconselhamento genético adequado.

Huntington disease (HD) is a progressive neurodegenerative disorder with autosomal dominant inheritance, characterized by choreiform movements and cognitive impairment. Onset of symptoms is around 40 years of age and progression to death occurs in approximately 10 to 15 years from the time of disease onset. HD is associated with an unstable CAG repeat expansion at the 5' and of the IT15 gene. We have genotyped the CAG repeat in the IT15 gene in 44 Brazilian individuals (42 patients and 2 unaffected family members) belonging to 34 unrelated families thought to segregate HD. We found one expanded CAG allele in 32 individuals (76%) belonging to 25 unrelated families. In these HD patients, expanded alleles varied from 43 to 73 CAG units and normal alleles varied from 18 to 26 CAGs. A significant negative correlation between age at onset of symptoms and size of the expanded CAG allele was found (r=0.6; p=0.0001); however, the size of the expanded CAG repeat could explain only about 40% of the variability in age at onset (r2=0.4). In addition, we genotyped 25 unrelated control individuals (total of 50 alleles) and found normal CAG repeats varying from 16 to 33 units. The percentage of heterozigocity of the normal allele in the control population was 88%. In conclusion, our results showed that not all patients with the "HD" phenotype carried the expansion at the IT15 gene. Furthermore, molecular diagnosis was possible in all individuals, since no alleles of intermediate size were found. Therefore, molecular confirmation of the clinical diagnosis in HD should be sought in all suspected patients, making it possible for adequate genetic counseling.