Abstract: Currently, the research of new natural compounds with biological potential demonstrates great ethnopharmacological importance. In this study, we evaluated the biological properties promoted by saline extract from Malpighia emarginata DC leaves, whose objective is to evaluate the antioxidant, antimicrobial and cytotoxicity potential. Phytochemical characterization was performed by UPLC-MS chromatography to identify the chemical compounds. For the antioxidant potential, DPPH, ATT and FRAP methods were used. The antibacterial and antifungal tests were performed evaluating the MIC50, MIC90, CMB and CMF parameters. Moreover, antibiofilm action was evaluated. Cytotoxicity and proliferation were performed using splenocytes from Balb/c mice and were evaluated by cytometry. We found a list of phenolic compounds among other bioactive compounds in the M. emarginata saline extract. In addition, higher antioxidant profile and antifungal activity against different strains of Candida spp. was promoted by the saline extract. Splenocytes showed greater cell viability (more than 90%) and showed higher proliferate index in 24 and 48 hours of incubation with the extract. Saline extract from Malpighia emarginata DC has potential action like antioxidant and antifungal agent without promote animal cell damage.

ABSTRACT Rhizophora mangle is an abundant plant in mangroves and tannic acid is a polyphenol produced by the secondary metabolism of plants. The aim of the study was to evaluate the embryotoxic and embriostatic effects of the aqueous extract of R. mangle and synthetic tannic acid on eggs and larvae of Aedes aegypti. A. aegypti eggs were exposed in duplicate at concentrations of 250, 500, 750 and 1000 µg/mL of extract and tannic acid for a period of 14 days. Mineral water was used as a negative control. The eggs were observed and counted in a stereomicroscope (1.2x). In all extract concentrations there was stimulation in hatching in relation to the control, but only in concentration of 750 mg/mL it was statistically significant. In tannic acid (250µg/ml) there was significant stimulus in hatching, but in 500, 750 and 1000 µg/mL there was significant inhibition. All concentrations of aqueous extract and tannic acid on larvae showed embryotoxic and embryostatic effects when compared to the control. The aqueous extract of R. mangle showed effect on hatching of A. aegypti eggs and synthetic tannic acid showed embryotoxic and embryostatic effects. On larvae, both the aqueous extract as tannic acid showed embryotoxic and embryostatic effects.

RESUMO Spondias tuberosa Arruda, popularmente conhecida como umbu, é uma espécie nativa da Caatinga amplamente utilizada na medicina popular, cujo perfil toxicológico ainda não é conhecido. Deste modo, este estudo avaliou o perfil fitoquímico, a toxicidade aguda e a citotoxicidade do extrato etanólico da casca da Spondias tuberosa Arruda (EEStb) nos parâmetros hematológicos, bioquímicos e histopatológicos. Foram utilizados ratos fêmeas Wistar (±210g) e divididos em: grupo controle (C) e tratados com uma dose única de 300mg/Kg (EEStb300) ou 2.000mg/kg do peso corporal (ESStb2.000) do EEStb. Após 24 horas e 14 dias da gavagem, os parâmetros comportamentais, hematológicos, bioquímicos e histopatológicos foram determinados. O efeito citotóxico foi avaliado em células HEp-2. Não foi observada mortalidade ou alterações de massa corporal durante os 14 dias de experimento nos grupos EEStb300 e EEStb2.000, mas EEStb2.000 provocou uma redução discreta no consumo hídrico e alimentar, assim como na atividade locomotora no 1º dia de tratamento. Não houve alterações nos parâmetros histopatológicos, macroscópicos, bioquímicos e hematológicos. EEStb2000 nas concentrações de 6.25-50μg.ml-1) não produziu efeito citotóxico. na célula HEp-2. Estes resultados sugerem que EEStb não promoveu toxicidade aguda ou citotóxica, sugerindo boa margem de segurança para uso da Spondias tuberosa Arruda.

ABSTRACT Spondias tuberosa Arruda, popularly named as umbu, is native from savanna-like vegetation and widely used for medicinal purposes, however, the toxicological profile is not available yet. This study evaluated the phytochemical profile and acute toxicity and citoxicity of Ethanolic Extract of Spondias tuberosa Arruda Bark (EEStb) in hematological, biochemical and histopathological parameters. Female Wistar rats were divided into: control (C) and animal treated single doses of 300mg/Kg (EEStb300) or 2.000mg/kg body weight (ESStb2.000) of the EEStb. After 24 hours and 14 days from gavage, the behavior, hematological, biochemical and histopathological parameters were assayed. Cytotoxicity effect was evaluated on HEp-2 cell lines. Neither EEStb300 nor EEStb2.000 produced mortality nor changes in body weight during the 14-days of observation, but EEStb2.000 reduced quietly the food and water intake as well as locomotor activity at first day. There were no changes in macroscopic, histopathological, biochemical and hematological parameters. EEStb in concentrations of 6.25- 50μg ml−1 on HEp-2 cell did not produce cytotoxic effect. These results suggest that EEStb did not cause acute toxicity and cytotoxic, suggesting a good safety rate for Spondias tuberosa Arruda.

O experimento foi realizado nas instalações experimentais da Faculdade de Medicina Veterinária e Zootecnia da Unesp, Campus de Botucatu e objetivou-se avaliar o efeito das dietas com milho e farelo de soja, sorgo com alto tanino e farelo de soja e sorgo com baixo tanino e farelo de soja sobre o desempenho, rendimento de carcaça e parâmetros gastrintestinais de frangos de corte. Foram utilizados 1200 pintos de corte de um dia, da linhagem Ross 308, distribuídos em 24 boxes em um delineamento experimental inteiramente casualizado em esquema fatorial 3 x 2, três dietas: 100% milho e farelo de soja, 100% sorgo com alto tanino e farelo de soja (SAT) e 100% sorgo com baixo tanino e farelo de soja (SBT) e dois sexos, com 4 repetições de 50 aves cada. O desempenho foi avaliado aos 21, 35 e 42 dias de idade e aos 42 dias de idade, uma amostra de 5 aves por repetição foi abatida para a determinação do rendimento de carcaça e das partes. Foram medidos o rendimento do peito desossado, coxa e sobrecoxa, asas, dorso e gordura abdominal. Aos 14, 21, 28, 35 e 42 dias de idade foram sacrificadas 3 aves por repetição para avaliação do desenvolvimento das vísceras e dos intestinos por meio de pesagens e medidas. Conclui-se que, ao utilizar sorgo com alto tanino (cultivar AG3002 com 1,89 g/kg) e sorgo com baixo tanino (cultivar SAARA com 0,49 g/kg) em substituição ao milho não foi encontrado efeito significativo (p>0,05) para os parâmetros analisados de desempenho e rendimento de carcaça, não ocorrendo também efeito evidente sobre a porcentagem das vísceras e intestinos e as medidas dos intestinos.

The experiment was carried out in the experimental facilities of Faculdade de Medicina Veterinária e Zootecnia of Unesp, Botucatu Campus and the objective was evaluate the effect of corn, sorghum with tannin and sorghum no tannin-based diets on the gastric and bowel parameters in broiler . One thousand and two hundred sexed Ross 308, one-day-old chicks were used, divided in 24 boxes in an completely randomized experimental design with factorial arrangement 3x2, three diets: 100% corn, 100% sorghum with tannin (SAT) and 100% sorghum without tannin (SBT) and two sexes, with 4 replications of 50 birds each The broiler performance was evaluate at 21, 35 and 42 days of age. At 42 days of age, a sample of 5 birds per replication was slaughtered to carcass yield determination.At 14, 21, 28, 35 and 42 days of age 3 birds per replication were slaughtered for the evaluation of guts and bowel development by means of weighing and measurements. It can be concluded that, when using sorghum with tannin (AG3002 crop with 1.89g/kg) and sorghum no tannin (SAARA crop, with 0.49g/kg) replacing corn, there was no evident effect (p>.05) on performance, carcass yield, as well as on guts and bowel percentage or on the bowel measures.