Abstract Glicophyllum is well supported, presenting four homoplasies, easily differentiated from the other genera of the clade due to characteristics related to the reproductive organs, which can make the identification of their species difficult when they are not in the reproductive phase. Therefore, there are provided the leaf anatomical and morphological description of the leaf glands of seven species of Glicophyllum to assist in the identification of their species. The samples for the study were obtained from several national and international herbaria, sectioned freehand, stained with basic fuchsin – astra blue and compared through a binary matrix using the Sorensen’s coefficient in the MVSP software. Among the leaf anatomical characters found, the following stand out: presence/absence of trichomes; petiole contour; contour of the main vein; organization of the mesophyll, presence/absence of bundle sheath extension and the surface of the glands. In this study, an identification key with leaf anatomical data is presented for the first time, demonstrating the applicability of leaf anatomy for the taxonomy of Glicophyllum. In the multivariate analysis, it is observed that the characteristics of leaf venation and topology of the glands are more representative to differentiate the taxa. Therefore, the data obtained can support future taxonomic and phylogenetic studies of the genus. supported homoplasies organs phase Therefore herbaria freehand Sorensens Sorensen s software found out presenceabsence presence absence trichomes vein mesophyll time analysis taxa genus

Abstract Being a food product that contains perishable ingredients and involves a significant degree of manual handling during preparation, sushi is regarded as a potentially hazardous food, which may lead to foodborne disease outbreaks. In Portugal, consumption of takeaway sushi meals has strongly increased throughout the past few years; however, there is limited information regarding its compliance with food quality standards. Under this context, the present study aimed to evaluate the microbiological quality and safety of take-away ready-to-eat sushi meals in Lisbon, Portugal. Sixty-two samples were collected from different origins (restaurant and hypermarket), and each sample was tested for aerobic mesophilic microorganisms, Enterobacteriaceae, Escherichia coli, positive coagulase Staphylococci, presumptive Bacillus cereus count, as for detection of pathogenic microorganisms, such as Salmonella spp., Listeria monocytogenes and Vibrio parahaemolyticus, V. cholerae and V. vulnificus. Results revealed that 48.4% (30/62) were deemed unsatisfactory, 35.5% (22/62) were classified as borderline and only 16.1% (10/62) were considered satisfactory. Even though we did not detect the incidence of potentially pathogenic microorganisms in sushi, the presence of B. cereus and coagulase-positive Staphylococci was detected at unsatisfactory levels. Furthermore, significant differences between the place of origin (restaurant vs. hypermarket) and type of fish were also observed. Overall, the high number of samples classified with a level of microbiological quality “unsatisfactory” and “borderline” highlights the need to review good hygiene practices, as well as the quality of the raw materials used, to obtain a final product with a satisfactory quality and safety level.

Resumo Por ser um género alimentício que contém ingredientes perecíveis e envolve um grau significativo de manipulação manual durante a sua preparação, o sushi é considerado um alimento potencialmente perigoso, que pode causar surtos de doença de origem alimentar. Em Portugal, o consumo de refeições de sushi prontas a consumir tem aumentado ao longo dos últimos anos. No entanto, a informação sobre o cumprimento das normas de qualidade alimentar é limitada. Neste contexto, o presente estudo teve como objetivo avaliar a qualidade e a segurança microbiológica de refeições de sushi prontas para consumo em take-way, na região de Lisboa, Portugal. Foram colhidas 62 amostras de diferentes origens (restaurante e hipermercado), e em cada amostra foi efetuada a contagem de microrganismos aeróbios mesófilos, Enterobacteriaceae, Escherichia coli, estafilococos coagulase positiva, Bacillus cereus presuntivos, e deteção de microrganismos patogénicos, tais como: Salmonella spp., Listeria monocytogenes, Vibrio parahaemolyticus, Vibrio cholerae e Vibrio vulnificus. Os resultados revelaram que 48,4% (30/62) das amostras foram consideradas insatisfatórias, 35,5% (22/62) foram classificadas como “borderline” e apenas 16,1% (10/62) foram consideradas como satisfatórias. Embora não tenham sido detetados microrganismos potencialmente patogénicos nas amostras de sushi, a presença de B. cereus e estafilococos coagulase positivos foram detetados em níveis insatisfatórios. Além disso, também foram observadas diferenças significativas entre o local de origem (restaurante vs. hipermercado) e tipo de peixe.No geral, o elevado número de amostras classificadas com um nível de qualidade microbiológica insatisfatória e “borderline” evidência a necessidade de revisão das boas práticas de higiene, bem como da qualidade das matérias-primas utilizadas, para obter um produto final com qualidade e segurança satisfatória.

Resumo Apiosordaria é um gênero de fungo com espécies geralmente reportadas habitando o solo ou fezes de herbívoros. No entanto, estudos referentes à associação de representantes deste gênero às plantas, especialmente em ecossistemas semiáridos, ainda são inexistentes. Neste trabalho documentamos uma nova ocorrência para o Brasil de uma espécie de Apiosordaria, associada a palmeira Syagrus coronata. As coletas foram realizadas na Estação Ecológica Raso da Catarina e as análises ocorreram no Laboratório de Ciências, da Universidade do Estado Bahia, Campus VIII, Paulo Afonso. A partir da análise topográfica do substrato vegetal em estereomicroscópio e da avaliação das estruturas fúngicas em microscópio óptico de luz, a espécie foi identificada como Apiosordaria nigeriensis. Este fungo foi relatado pela primeira vez em Enugu, na Nigéria, após de ter sido isolado de amostras de solo. Neste trabalho, reportamos a segunda ocorrência de A. nigeriensis para o mundo, sendo a primeira para o Brasil, representando o primeiro registro para as Américas, e dando destaque a palmeira S. coronata como o primeiro hospedeiro botânico colonizado pela espécie. Estes dados ampliam o conhecimento sobre da distribuição geográfica do gênero, em especial sobre a micodiversidade para o bioma caatinga.

Abstract Apiosordaria is a genus of fungus with species commonly reported inhabiting soil or herbivore feces. However, studies related to the association of representatives of this genus to plants, especially in semi-arid ecosystems, are still non-existent. In this work we documented a new occurrence for Brazil of a species of Apiosordaria, associated with the palm Syagrus coronata. The collections were carried out at the Raso da Catarina Ecological Station and the analyzes were carried out at the Laboratory of Sciences of the Bahia State University, Campus VIII, Paulo Afonso. From the topographic analysis of the plant substrate in stereomicroscope and the evaluation of fungal structures under optical light microscope, the species was identified as Apiosordaria nigeriensis. This fungus was reported for the first time in Enugu, Nigeria, after being isolated from soil samples. In this work, we report the second occurrence of A. nigeriensis to the world, being the first to Brazil, representing the first record for the Americas, and highlighting the palm S. coronata as the first botanical host colonized by the species. These data broaden the knowledge about the geographic distribution of the genus, especially on the microdiversity for the caatinga biome.

Abstract Introduction There is a mechanism of macrolide resistance in Staphylococcus spp. which also affects the lincosamides and type B streptogramins characterizing the so-called MLSB resistance, whose expression can be constitutive (cMLSB) or inducible (iMLSB) and is encoded mainly by ermA and ermC genes. The cMLSB resistance is easily detected by susceptibility testing used in the laboratory routine, but iMLSB resistance is not. Therapy with clindamycin in cases of infection with isolated iMLSB resistance may fail. Objective To characterize the phenotypic (occurrence of cMLSB and iMLSB phenotypes) and molecular (occurrence of ermA and ermC genes) profiles of MLSB resistance of clinical isolates of susceptible and methicillin-resistant Staphylococcus aureus and CNS (coagulase-negative Staphylococcus) from patients of a university hospital, in Pernambuco. Methods The antimicrobial susceptibility of 103 isolates was determined by the disk diffusion technique in Mueller–Hinton agar followed by oxacillin screening. The iMLSB phenotype was detected by D test. Isolates with cMLSB and iMLSB phenotypes were subjected to polymerase chain reaction (PCR) for the detection of ermA and ermC genes. Results The cMLSB and iMLSB phenotypes were respectively identified in 39 (37.9%) and five (4.9%) isolates. The iMLSB phenotype was found only in four (10.8%) methicillin-susceptible S. aureus and one (4.5%) methicillin-resistant S. aureus. In the 44 isolates subjected to PCR, four (9.1%) only ermA gene was detected, a lower frequency when compared to only ermC 17 (38.6%) gene and to one (2.3%) isolate presenting both genes. Conclusion In the Staphylococcus spp. analyzed, the ermC gene was found more often than the ermA, although the iMLSB phenotype had been less frequent than the cMLSB. It was important to perform the D test for its detection to guide therapeutic approaches.

ABSTRACT White mold caused by the fungus Sclerotinia sclerotiorum is the most yield-limiting disease of common bean in Brazil. To date, there has been no commercial cultivar resistant to this disease. In a greenhouse we evaluated white mold resistance sources (Cornell 605, A195 and G122) against eight isolates of S. sclerotiorum from five Brazilian states. A Brazilian cultivar (BRSMG Madrepérola) and a susceptible check (Beryl) were used as control. Treatments were arranged in factorial combinations (5 × 8) in a completely random design with four replicates. Disease severity was assessed on a rating scale of 1-to-9 together with lesion length, which was used to determine an area under the disease progress curve (AUDPC). Polymorphisms detected in ten microsatellite loci were used to assess variability between the isolates. Each isolate was a distinct haplotype; they formed a genetic tree with two clusters. One cluster was formed by three isolates collected from the states of Minas Gerais and São Paulo (southeastern); the others, by isolates from Paraná, Santa Catarina (southern), Goiás (Mid-western), and again, Minas Gerais. Genotype × isolate interaction was significant. In general, Beryl was more susceptible than BRSMG Madrepérola. Considering the AUDPC and/or the white mold reaction score, Cornell 605 exhibited more physiological resistance than BRSMG Madrepérola to seven isolates, A195 to five isolates, and G122 to two isolates. Our results suggest that Cornell 605 is the best source of resistance to white mold for the southern region, whereas Cornell 605 and A195 are somewhat superior to G122 for the southeastern and mid-western regions.

The purpose of this study was to determine the Minimum Inhibitory Concentration (MIC) of pure or mixed chemicals for Saccharomyces cerevisiae and Lactobacillus fermentum in the samples isolated from distilleries with serious bacterial contamination problems. The biocides, which showed the best results were: 3,4,4' trichlorocarbanilide (TCC), tested at pH 4.0 (MIC = 3.12 mg/l), TCC with benzethonium chloride (CBe) at pH 6.0 (MIC = 3.12 mg/l) and TCC mixed with benzalkonium chloride (CBa) at pH 6.0 (MIC = 1.53 mg /l). If CBa was used in sugar cane milling in 1:1 ratio with TCC, a 8 times reduction of CBa was possible. This formulation also should be tested in fermentation steps since it was more difficult for the bacterium to develop resistance to biocide. There was no inhibition of S. cerevisiae and there were only antibiotics as an option to bacterial control of fuel ethanol fermentation by S. cerevisiae.

There are many controversies regarding the cyto- and genotoxicity of carbon nanotubes (CNTs). In this work, we discuss that many of the incongruous arguments are probably associated with the poor physical-chemical characterization of the CNT samples used in many publications. This manuscript presents examples of carbon nanostructures observed under high resolution electron microscopy that can be found in typical CNT samples, and shows which roles in cyto- and genotoxicity need to be better investigated. Issues concerning chemical treatment are addressed and examples of misunderstandings that can occur during the studies of cyto- and genotoxicity of CNT samples are given.

RESUMO O objetivo deste trabalho foi avaliar o efeito de doses de macronutrientes sobre o crescimento e os índices de qualidade de mudas de canafístula e determinar a dose recomendada dos mesmos para estabelecer um adequado programa de adubação. No trabalho, conduzido em casa de vegetação, utilizou-se amostras de Argissolo Vermelho Amarelo, retirado da camada abaixo de 20 cm de profundidade, como substrato. Delimitou-se o experimento por meio de matriz baconiana, avaliando-se três doses dos seis macronutrientes (nitrogênio, fósforo, potássio, cálcio, magnésio e enxofre) e dois tratamentos adicionais, um com doses de referência e outro sem adição de nutrientes. Adotou-se o delineamento inteiramente casualizado com quatro repetições. Foram analisados os parâmetros morfológicos e os índices de qualidade de mudas. Verificou- se, através de análise dos contrastes ortogonais, resposta significativa de todas as variáveis estudadas à aplicação dos macronutrientes, exceto para a relação altura diâmetro (H/D) nos tratamentos com P, Ca e S. Não foi verificada resposta à aplicação de Ca para a maioria das características avaliadas, com exceção das relações altura de parte aérea por peso de matéria seca de parte aérea (H/MSPA) e peso de matéria seca de parte aérea por peso de matéria seca de raiz (MSPA/MSR). Recomenda-se a aplicação de 600 mg dm-3 de P, 150 mg dm-3 de K, 0,50 cmol dm-3 de Mg e 50 mg dm-3 de S, e sugerem-se novos estudos com os nutrientes N e Ca, para a produção de mudas de canafístula, usando a camada abaixo de 20 cm de profundidade de Argissolo Vermelho Amarelo como substrato.

ABSTRACT The objective of this work was to evaluate the effect of macronutrients doses upon the growth and quality indices of Peltophorum dubium seedlings and to determine the recommended doses for them to establish a suitable fertilization program. In the paper, carried out in a greenhouse, samples of ‘Red Yellow Ultisol’ were used, removed from the layer below, 20 cm deep, as a substratum. The experiment was designed in a Baconian Matrix, and three doses of the six macronutrients (nitrogen, phosphorus, potassium, calcium, magnesium and sulfur) and two additional treatments were evaluated, one with reference doses and the other one without the addition of nutrients. The entirely casual design was adopted, with four repetitions. The morphologic parameters and quality indices of seedlings were analyzed. Through the orthogonal contrasts, a significant response of all the variables studied was verified related to the macronutrient applied, except for the relation height/diameter (H/D) in the treatments with Ca, P and S. It was not verified response to Ca application for most characteristics evaluated, unless for the relation of the aerial part height per aerial part dry weight (H/MSPA) and aerial part dry weight per root dry weight (MSPA/MSR). The recommended doses were of 600 mg dm-3 of P, 150 mg dm-3 of K, 0,50 cmol dm-3 of Mg and 50 mg dm-3 of S. Based on this work, further studies are suggested with N and Ca, for seedlings production of the studied species using from the layer below 20 cm deep of the ‘Red Yellow Ultisol’ as the substratum.

Este trabalho teve como objetivo avaliar a viabilidade de um isolado de Tritrichomonas foetus criopreservada com glicerol e dimetilsulfóxido (DMSO) a -196ºC. Isolados do protozoário foram descongeladas dois dias após o congelamento e 6, 12, 18 e 26 meses, para avaliação de sua viabilidade. Em todos os tempos analisados, o congelamento foi viável em 60% e 90% das amostras congeladas com glicerol a 10% e DMSO a 12%, respectivamente.

The aim of this work was to evaluate the viabilitity of Tritrichomonas foetus cryopreservated with glycerol and dimethilsulphosyde (DMSO) at -196ºC. Samples of the protozoa were thaw two days and 6, 12, 18, and 26 months after freezing to be evaluated. In the time analyzed, the freezing was viable in 60% and 90% of freeze samples with glycerol at 10% and DMSO at 12%, respectively.

É mostrada a importância atual da atividade de "notificação de barbeiros" pela população, na vigilância epidemiológica da doença de Chagas no Estado de São Paulo. São estudadas as variações ocorridas no número de notificações encaminhadas pela população no período de 1974 a 1983, relativa a uma área da Região Nordeste do referido Estado, com infestação por Panstrongylus megistus. Observou-se que a população responde aos estímulos à sua participação. Verificou-se aumento do número de notificações a partir do mês de outubro, mantendo-se similar nos meses de novembro e dezembro. Não foi encontrada relação entre os índices de infestação e o de notificação.

The importance of "bug reporting", on the part of the local human population, to the epidemiological surveillance of Chagas' disease in S. Paulo State is demonstrated. The variations the number of "bug reports" from the local human population in the period from 1974 to 1983 relating to an area, infested by Panstrongylus megistus, in the northeast of S. Paulo State are studied. An increase in the number of "bug reporting" occurring in October, November and December was observed; however, no connection was discovered between domiciliary infestation rate and the "bug report" rate in this specific area.

É mostrada a importância atual da atividade de "notificação de barbeiros" pela população, na vigilância epidemiológica da doença de Chagas no Estado de São Paulo. São estudadas as variações ocorridas no número de notificações encaminhadas pela população no período de 1974 a 1983, relativa a uma área da Região Nordeste do referido Estado, com infestação por Panstrongylus megistus. Observou-se que a população responde aos estímulos à sua participação. Verificou-se aumento do número de notificações a partir do mês de outubro, mantendo-se similar nos meses de novembro e dezembro. Não foi encontrada relação entre os índices de infestação e o de notificação.

The importance of "bug reporting", on the part of the local human population, to the epidemiological surveillance of Chagas' disease in S. Paulo State is demonstrated. The variations the number of "bug reports" from the local human population in the period from 1974 to 1983 relating to an area, infested by Panstrongylus megistus, in the northeast of S. Paulo State are studied. An increase in the number of "bug reporting" occurring in October, November and December was observed; however, no connection was discovered between domiciliary infestation rate and the "bug report" rate in this specific area.