Abstract The objective of this study is to describe the agronomic attributes of the SCS443 Isadora apple tree cultivar and to highlight its potential use in Brazil. Obtained by the hybridization made at Epagri in 2001 between ‘Imperatriz’ and ‘Cripps Pink’, after 20 years of agronomic evaluations carried out in Caçador-SC and Fraiburgo-SC, ‘SCS443 Isadora’ was originated. This cultivar has a wide plasticity of adaptation to the southern Brazilian climate due to the medium chilling requirement to overcome dormancy. It has greater resistance to the main apple diseases than cv. Gala and fruit harvest is later than cv. Fuji. Its apples have a red skin and firm flesh (=18 lb), with high sugar content (= 14.5 %) and very crunchy and juicy texture, as well as high storage ability in post-harvesting. ‘SCS443 Isadora’ apples can be stored for 8 months, even in refrigerated air atmosphere chambers (21% O2) with inhibition of the ethylene response by the use of 1-methylcyclopropene (1-MCP). Even stored for long periods of time, the indices of quality loss, physiological disorders and fruit rotting in ‘SCS443 Isadora’ apples are lower than on Gala and Fuji apples.

Resumo O trabalho tem como objetivo descrever os atributos agronômicos da cultivar de macieira SCS443 Isadora e evidenciar suas potencialidades de uso no Brasil. Obtida pela hibridação realizada na Epagri, em 2001, entre ‘Imperatriz’ e ‘Cripps Pink’, após 20 anos de avaliações agronômicas realizadas em Caçador-SC e Fraiburgo-SC, originou-se a ‘SCS443 Isadora’. Esta cultivar é de ampla plasticidade de adaptação ao clima sul-brasileiro, em função do médio requerimento de frio hibernal, para superação da dormência das gemas. Possui maior resistência às principais doenças da macieira que a cv. Gala, e a maturação dos frutos é mais tardia que da cv. Fuji. As suas maçãs são de epiderme vermelha e de polpa firme (=18 lb.),com alto teor de açúcares (= 14,5 %) e de textura muito crocante e suculenta, bem como de muito alta capacidade de conservação em câmara fria. Maçãs ‘SCS443 Isadora’podem ser armazenadas por 8 meses, mesmo em câmaras de atmosfera do ar refrigerado (21% de O2), com a inibição da resposta ao etileno pelo uso do 1-methylcyclopropene(1-MCP). Mesmo armazenadas por longos períodos de tempo, os índices de perda de qualidade, dedistúrbios fisiológicos e de podridões são inferiores aos das maçãs Gala e Fuji.

Abstract According to literature, the apple cultivar SCS425 Luiza is a hybrid of Imperatriz♀ and Cripps Pink♂; however, molecular analysis of gametophytic self-incompatibility alleles showed the presence of an unexpected allele in SCS425 Luiza. This raised doubts about the fidelity of the pedigree. Thus, this study aimed to investigate the real genealogy of the SCS425 Luiza cultivar via fingerprint analysis. A total of 19 pairs of SSR primers covering 12 of the 17 chromosomes were used. ‘Imperatriz’ was tested as the female parent, and either ‘Cripps Pink’ or ‘Baronesa’ was tested as the presumed male parents. The results excluded ‘Cripps Pink’ as the male parent because most of the markers showed alleles of exclusion with Likelihood ratio (LR) value equal to zero. In contrast, when Baronesa was tested as the male parent, all alleles followed the expected segregation with very high LR values (>10.000). Therefore, it is concluded that the correct genealogy of the SCS425 Luiza cultivar is Imperatriz♀ and Baronesa♂.

Resumo O objetivo deste trabalho foi caracterizar genitores e respectivas populações de macieiras quanto aos alelos S para confirmar sua genealogia e avaliar a eficiência dos marcadores moleculares utilizados. Conjuntos específicos de iniciadores foram utilizados para a identificação dos alelos S via PCR. Foram avaliadas duas populações segregantes do Programa de Melhoramento Genético de Macieira da Epagri resultantes dos cruzamentos entre ‘Fred Hough’ × ‘Monalisa’ e ‘M-11/00’ × ‘M-13/91’. As segregações esperadas são 1:1:1:1 para compatibilidade total e 1:1 para semi-compatibilidade, que pode ser confirmada pelo teste X2. O cruzamento ‘Fred Hough’ (S5S19) × ‘Monalisa’ (S2S10) foi identificado como totalmente compatível, e foram identificados dois triploides entre os híbridos. O cruzamento entre ‘M-11/00’ (S3S19) × ‘M-13/91’ (S3S5) se mostrou semi-compatível baseado nos marcadores moleculares e a segregação dos alelos S nos híbridos foi de 1:1 como esperado. A segregação dos marcadores de DNA para S2, S3, S5, S10 e S19 ocorreu juntamente com seus respectivos alelos S. Dessa forma, a caracterização dos alelos S, além de permitir identificar a compatibilidade entre os genitores, serviu para identificar contaminações em populações segregantes.

Abstract The objective of this study was to characterize the parents and respective populations of apple trees regarding S-alleles to confirm their genealogy and to evaluate the efficiency of the molecular markers used. Sixteen specific sets of primers were used for identification of apple S-alleles by PCR. Two segregating populations of the Epagri Apple Breeding Program resulting from crosses between ‘Fred Hough’ × ‘Monalisa’ and ‘M-11/00’ × ‘M-13/91’ were evaluated. The expected segregations are 1:1:1:1 for full compatibility and 1:1 for semi-compatibility, which can be confirmed by the X2 test. The ‘Fred Hough’ (S5S19) × ‘Monalisa’ (S2S10) cross proved to be fully compatible; and two triploids were identified among the hybrids as well. The ‘M-11/00’ (S3S19) × ‘M-13/91’ (S3S5) cross was characterized as semi-compatible based on DNA markers, and the segregation of the S-alleles in the hybrids was 1:1, as expected. The segregation of the DNA markers occurred together with their respective S-alleles: S2, S3, S5, S10, and S19. Thus, characterization of the S-alleles not only allowed identification of compatibility between parents but also identified contaminations in segregating populations.

ABSTRACT Apple orchards require the presence of a different genotype to pollinate the fruit producing cultivar. This is due to the process of gametophytic self-incompatibility present in most species and cultivars of the genus Malus. The fruit producing cultivar and the pollinizer must be genetically compatible to ensure fruit set and symmetrical and adequate fruit formation. The aim of this work was to evaluate five potential pollinizers for the new apple cultivar SCS426 Venice by genotyping the self-incompatibility locus (S-locus) and by controlled pollination in the field. The S-locus was screened using molecular markers and the fertilization capacity was evaluated by monitoring the fruit set after artificial pollination. Three genotypes were identified as semi-compatible (selection 135/140, cultivar SCS433 Felix 3 and SCS425 Luiza) and two as fully compatible (‘SCS431 Felix 1’ and ‘SCS434 Felix 4’) with ‘SCS426 Venice’. Regardless of the level of compatibility, all genotypes tested are efficient for the fertilization of ‘SCS426 Venice’ flowers and can be used as pollinizers in commercial orchards of this cultivar.

Abstract The Apple Germplasm Bank (AGB) of Santa Catarina Agricultural Research and Rural Extension Company - Epagri, AGB-Epagri, is the largest of the genus Malus in Brazil. Twenty-eight main accessions of this bank were virus screened through DAS-ELISA, RT-PCR and IC-RT-PCR during two consecutive reproductive cycles, and each accession showed latent mixed infection by at least two species, among ASGV, ASPV and ACLSV. The combined use of diagnostic methods helped overcome inconsistencies commonly found in apple virus detection and was shown essential for the AGB-Epagri can be safely used as a source of genetic variability and for the exchange of virus-free propagative material.

Abstract: The objective of this study was to identify self-incompatibility (S) alleles of advanced breeding selections of apple (Malus × domestica Borkh.). The S-alleles of 42 apple genotypes were analyzed by markers using allele-specific PCR amplification and amplicons digested with restriction endonucleases. Among the screened genotypes were cultivars, advanced selections, and accessions of the Apple Germplasm Bank of Epagri (Caçador, Santa Catarina, Brazil). Two S-alleles were identified in 36 genotypes, and only one S-allele was determined in the other six genotypes. In all, eleven S-alleles were identified among all the genotypes evaluated. The S3 and S5 alleles were most frequent (30.2% and 19.8%, respectively). The identification of S-alleles using molecular markers in important apple tree genotypes is useful for determination of compatible parents for breeding programs.

Abstract SCS426 Venice was developed by hybridization between Epagri 404-Imperatriz (♀) and Epagri 406-Baronesa (♂), performed at the Epagri Experimental Station in Caçador, SC, Brazil, in 2001. It requires less winter chilling than ‘Gala’ and ‘Fuji’ traditional clones, whose fruit is picked in March. It is resistant to glomerella leaf spot.

Abstract The E’AppleBP is the largest Brazilian apple breeding program in activity in Brazil, with Brazilian and international contributions to breeding of apple cultivars, under public funding. The main objectives are development of new apple cultivars with good local climate adaptation, disease resistance, high yield, high fruit quality, good fruit storability and lower demand for orchard hand labor. Twenty-seven apple cultivars have been released, including 15 from local breeding crosses and four sport mutations. ‘Fuji Suprema’, ‘Monalisa’, ‘Venice’, ‘Daiane’, ‘Luiza’, and ‘Kinkas’ are most promising for commercial use. The other eight cultivars were released for use as pollinizers.

Resumo A maçã é um dos principais produtos do agronegócio da fruticultura temperada do Sul do Brasil. Mais de 90% da produção são originadas com o cultivo de apenas duas cultivares – Gala e Fuji, e seus mutantes. Essas cultivares são pouco adaptadas ao clima regional, sendo as do grupo ‘Gala’ também suscetíveis à doença mancha foliar de glomerella - MFG, atualmente a principal doença da macieira no Brasil. A ‘SCS425 Luiza’ foi desenvolvida pela Epagri / Estação Experimental de Caçador, buscando disponibilizar à cadeia produtiva mais uma cultivar de macieira precoce, mais bem adaptada aos ambientes de cultivo sul-brasileiros e resistente à MFG. Foi obtida via hibridação artificial, realizada em 2001, tendo como parentais as cultivares Imperatriz e Cripps Pink. A ‘SCS425 Luiza’ apresenta menor requerimento em frio hibernal que a ‘Galaxy’, tem maior precocidade em iniciar a floração e possui produção ao menos similar à da ‘Galaxy’. Produz frutos com qualidade visual, sabor e textura de polpa adequados para o mercado brasileiro e com capacidade de conservação similar à ‘Galaxy’ quando colhida no mesmo estágio de maturação.

Abstract Apple is one of the main agribusiness products of temperate-zone fruits in Southern Brazil. More than 90% of apple production is obtained from only two apple cultivars – Gala and Fuji and their mutations. These apple varieties are insufficiently adapted to the regional climate and ‘Gala’ and its mutations are also susceptible to glomerella leaf spot – GLS, currently the main apple disease in Brazil. ‘SCS425 Luiza’ cultivar was developed by Epagri / Estação Experimental Caçador, aiming to offer to the production chain an early apple variety better adapted to the environmental conditions of Southern Brazil and resistant to GLS. ‘SCS425 Luiza’ cultivar was obtained by artificial hybridization carried out in 2001 using the cultivars Imperatriz and Cripps Pink as parents. ‘SCS425 Luiza’ has lower chilling requirement compared to ‘Galaxy’, higher flowering precocity and yield potential at least equivalent to ‘Galaxy’. It produces fruits with skin finish, flavor and flesh texture adequate for the Brazilian market and storability similar to ‘Galaxy’ apples when harvested at the same maturity.

Resumo: O objetivo deste trabalho foi identificar porta-enxertos com potencial agronômico competitivo para substituir os tradicionalmente usados no Sul do Brasil para as cultivares-copa de macieira Galaxy e Fuji Suprema cultivadas em solo de replantio. O experimento foi conduzido em Lebon Regis, no Estado de Santa Catarina, nas safras de 2009/2010 a 2014/2015, em delineamento experimental de blocos ao acaso, com quatro repetições. Avaliou-se o desempenho agronômico das copas 'Galaxy' e 'Fuji Suprema', enxertadas sobre G.056, G.202, G.213, G.814, G.896, G.969, M.9 e Marubakaido/M.9. G.896 e G.814 se destacaram pelas produções de frutos anual e acumulada superiores, bem como pela maior regularidade produtiva, embora tenham apresentado menor capacidade ananizante sobre a copa. As maiores massas médias de frutos de 'Galaxy' foram obtidas sobre G.056 e G.213. Para 'Fuji Suprema', G.969 proporcionou frutos menores, enquanto os demais porta-enxertos não diferiram entre si. Todos os porta-enxertos avaliados rebrotaram menos que Marubakaido/M.9 e produziram menos “burrknots” que M.9 e Marubakaido/M.9. No geral, nas condições de solo de replantio, G.202 mostra ser promissor entre os porta-enxertos ananizantes, principalmente para 'Fuji Suprema'. Destacam-se também G.056, G.814 e G.896, sendo os dois primeiros mais específicos para 'Fuji Suprema'.

Abstract: The objective of this work was to identify rootstocks with competitive agronomic potential to substitute those currently used in Southern Brazil for the Galaxy and Fuji Suprema apple scion cultivars cultivated on replanting soils. The experiment was carried out in the municipality of Lebon Regis, in the state of Santa Catarina, Brazil, from the 2009/2010 to the 2014/2015 crop season, in a randomized complete block design, with four replicates. The yield performance of the 'Galaxy' and 'Fuji Suprema' scions grafted on the G.056, G.202, G.213, G.814, G.896, G.969, M.9, and Marubakaido/M.9 rootstocks was evaluated. G.896 and G.814 stood out due to their superior annual and cumulative fruit yields, as well as to their greater yield regularity, although they showed lower ability of dwarfing the scion. The greatest average fruit weight of 'Galaxy' was obtained on G.056 and G.213. For 'Fuji Suprema', G.969 promoted smaller fruits, whereas the other rootstocks did not differ. All the evaluated rootstocks produced less suckers than Marubakaido/M.9 and less burrknots than M.9 and Marubakaido/M.9. In general, on replanting soil conditions, G.202 is promising among dwarfing rootstocks, mainly for 'Fuji Suprema'. G.056, G.814, and G.896 also stand out, and the two first are more specific for 'Fuji Suprema'.

Resumo O objetivo deste trabalho foi avaliar o potencial de conservação de maçãs da cultivar ‘SCS426 Venice’ submetidas a diferentes tecnologias de armazenagem. Os frutos foram colhidos em um pomar experimental localizado no município de Fraiburgo-SC, e armazenados por até oito e dez meses, nos anos de 2013 e 2014, respectivamente. As maçãs foram tratadas ou não com 1-metilciclopropeno (1-MCP) e armazenadas em atmosfera do ar (AA; 0,5±0,5 °C/UR de 85±5%) ou em atmosfera controlada (AC; 1,5 kPa O2 e 1,5 kPa CO2 a 0,7±0,5 °C/UR de 93±3%). Os frutos foram analisados a cada dois meses de armazenagem, logo após a retirada da câmara e após sete dias em condição ambiente (23±0,3 °C/UR de 68±0,6%). O armazenamento de maçãs ‘SCS426 Venice’ sob AA e sem aplicação de 1-MCP não deve estender-se além de seis meses. Nessa condição de armazenagem, as maçãs apresentam maior incidência de podridões e maiores taxas respiratória e de produção de etileno, bem como uma coloração da epiderme mais amarelada, menor firmeza de polpa e menor acidez titulável e teor de sólidos solúveis, em comparação aos frutos armazenados em AC ou em AA tratados com 1-MCP. Maçãs ‘SCS426 Venice’ podem desenvolver escurecimento de polpa e escaldadura superficial depois de longos períodos de armazenagem. Maçãs ‘SCS426 Venice’ em AA, tratadas com 1-MCP ou em AC, independentemente da aplicação de 1-MCP, apresentam potencial de armazenagem superior a oito meses, mantendo firmeza superior a 14 lb e baixo índice de distúrbios fisiológicos, mesmo após dez meses de armazenagem.

Abstract This study aimed to evaluate the storage potential of SCS426 Venice apples under different storage technologies. Fruits were harvested in an experimental orchard located in Fraiburgo, SC and stored for up to eight and ten months in 2013 and 2014, respectively. Apples were treated or not with methylcyclopropene (1-MCP) and stored under air atmosphere (AA, 0.5±0.5 °C/RH 85±5%) or controlled atmosphere (CA; 1.5 kPa of O2 and 1.5 kPa of CO2 at 0.7±0.5 °C/RH of 93±3%). Fruits were evaluated every two months of storage, after one and seven days of shelf life (23 ± 0.3 °C/RH 93±3%). The storage period of ‘SCS426 Venice’ apples under AA without 1-MCP application should not extend beyond six months. Under this storage condition, fruits had higher incidence of decay, ethylene production and respiratory rates, higher skin degreening, lower flesh firmness, titratable acidity and soluble solids content than fruits stored under CA or AA with 1-MCP. ‘SCS426 Venice’ apples develop flesh browning and superficial scald after long-term storage. ‘SCS426 Venice’ apples under AA treated with 1-MCP or under CA (regardless of 1-MCP application) can be stored for more than eight months, keeping flesh firmness above 14 lb and low incidence of physiological disorders even after ten months of storage.

Abstract The objective of this study was to evaluate the performance of Geneva(r) apple rootstocks for 'Gala'. The experiment was set in a complete randomized block design with four replications of tree plants per plot. It was used ten Geneva(r) rootstocks and M.9EMLA and M.7EMLA as controls. It was evaluated plant vigor, annual yield, cumulative yield, cumulative yield efficiency and fruit weight. For 'Gala' vigor control, G.10 and G.757 were more efficient than M.9EMLA; CG.2022 was equivalent and the other rootstocks were less efficient. 'G.24', 'G.969', 'G.30' and 'G.210' presented vigor equivalent to 'M.7EMLA'. 'G.896' was the most vigorous. Among dwarfing rootstocks, G.213 and G.757 were the most efficient on cumulative yield, the last showing the highest cumulative yield efficiency. Among semi-dwarfing, G.210 showed the highest cumulative yield and the highest cumulative yield efficiency. 'G.896' induced higher cumulative yield than 'M.7EMLA'. The greatest 'Gala' fruit weight was on 'G.213' and 'CG.2022'.

RESUMOEste estudo teve como objetivo verificar o desempenho agronômico de porta-enxertos de macieira da série Geneva® sobre o controle do vigor, a produção anual e acumulada, a eficiência produtiva e a massa fresca média de frutos das cultivares Gala e Fuji, em relação aos porta-enxertos tradicionalmente usados no meio-oeste de Santa Catarina, M.9 e MM.111. Os porta-enxertos da série Geneva® foram mais eficientes no controle do vigor e na eficiência produtiva da ‘Gala’ e da ‘Fuji’ que o MM.111. Todos induziram maior produção acumulada que o M.9, com maior destaque ao ‘G.213’ e ao ‘G.210’ para as duas cultivares; e ao ‘G.202’ para a ‘Fuji’. Em eficiência produtiva, todos foram superiores ao ‘MM.111’. O ‘G.213’ e o ‘CG.22’ foram mais eficientes que o ‘M.9’ para a ‘Gala’. Para a ‘Fuji’, todos foram mais eficientes que o ‘M.9’, com exceção do ‘CG.22’. Considerando todas as características avaliadas, os porta-enxertos G.213 (ananizante) e G.210 (semiananizante) são as opções mais promissoras de novos porta-enxertos para ambas as cultivares de macieira avaliadas. O G.202 também se mostrou promissor para a ‘Fuji’.

ABSTRACTThe purpose of this study was to evaluate the agronomic performance of some Geneva® apple rootstocks on plant vigor control, annual yield and cumulative yield, cumulative yield efficiency and fruit weight of ‘Gala’ and ‘Fuji’ scions compared to the traditional apple rootstocks ‘M.9’ e ‘MM.111’, used in Brazil. All the Geneva® apple rootstocks were more efficient than MM.111 on vigor control and cumulative yield efficiency of ‘Gala’ and ‘Fuji’ in the Middle West of the state of Santa Catarina. All of them induced higher cumulative yield than the ‘M.9’, mainly for the ‘G.213’ and the ‘G.210’ for both varieties, and the ‘G.202’ for ‘Fuji’. All the Geneva® rootstocks had more cumulative yield efficiency than ‘MM.111’. The ‘G.213’ and ‘CG.22’ were more efficient than ‘M.9’ for ‘Gala’. For ‘Fuji’, all Geneva® rootstocks were more efficient than ‘M.9’, except for ‘CG.22’. Considering all characteristics evaluated, G.213 (dwarfing) and G.210 (semidwarfing) were the most promising rootstocks for both scion varieties. The rootstock G.202 was very promising to ‘Fuji’ as well.

A garantia de boas produções em pomares comerciais de macieira depende de uma polinização eficiente, que também está relacionada com a compatibilidade de pólen-estigma, com a coincidência de época de floração e com a capacidade de produção e de germinação de pólen. O objetivo deste estudo foi avaliar a eficiência de diferentes genótipos de macieira como polinizadores da cv. Daiane nas condições climáticas do meio-oeste de Santa Catarina. Plantas de 'Daiane' foram polinizadas a campo com pólen de diversas seleções e cultivares de macieira, com subsequente proteção dos cachos com sacos de papel, por 72 h. Consideraram-se a coincidência de época de floração, a adaptação climática, a taxa de germinação, a reação à mancha foliar de Glomerella, além da frutificação efetiva e do número de sementes por fruto induzido pelas polinizadoras avaliadas. Os tratamentos mais eficientes na polinização da cultivar de macieira Daiane foram as seleções 140/76 e 140/228, respectivamente, que são indicadas para utilização conjugada, visando à maior eficiência na polinização.

Ensuring good production in apple orchards depends on efficient pollination, which is directly related to pollen-stigma compatibility, blooming time coincidence, high production and good germination capacity of pollen. The aim of this study was to evaluate the efficiency of different apple genotypes as pollinizers of 'Daiane' cv. at the climate of Middle-west of the state of Santa Catarina, Brazil. Apple trees of 'Daiane' cv. were pollinated in the field with several apple selections from the Apple Breeding Program of Epagri - Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina. Right after pollination, the flowering clusters were protected with paper bags for 72h hours. It were considered the coincidence on blooming time, the climatic adaptation of the trees, the percentage of germination, the reaction to Gala Leaf Spot disease, as well as the fruit set and the number of seed per fruit induced by the pollinizers of this study. It was observed that the best pollinizers for 'Daiane' apple trees were the selections 140/76 and 140/228, respectively. Both selections are indicated to be used combined as pollinizers of 'Daiane'.

A divergência genética entre 14 cultivares de mandioca-de-mesa foi estimada mediante o uso de caracteres morfoagronômicos e de marcadores moleculares RAPD. As análises de agrupamento de Tocher e do Vizinho Mais Próximo foram efetuadas. As cultivares mais divergentes foram Pão e Guaíra, Fécula Branca e Pão, e Pão e Caipira, enquanto as mais similares foram Fécula Branca e Branca 1, Branca 3 e Branca 1, e Guaíra e Branca 1. O coeficiente de Jaccard indicou que as cultivares mais similares foram Guaíra e Quarenta Quilos, enquanto as mais dissimilares foram Branca 3 e Amarela da Rama Cinza. A análise de divergência indicou que cruzamentos promissores deveriam ser efetuados entre as cultivares Branca 3, Pão, Amarela 1, Fécula Branca e Amarela 2 pela alta divergência genética, características agronômicas e culinárias favoráveis, e resistência à doenças de parte de pelo menos um dos parentais envolvidos no cruzamento.

In this work the genetic divergence among 14 sweet cassava cultivars was estimated by their morphological agronomic traits and RAPD molecular markers. The Tocher cluster analysis and the Nearest Neighbor Method were applied. The most dissimilar cultivars were Pão and Guaíra, Fécula Branca and Pão, and Pão and Caipira, while the most similar cultivar were the Fécula Branca and Branca 1, Branca 3 and Branca 1, and Guaíra and Branca 1. The Jaccard's coefficient showed that the most similar cultivars were Guaíra and Quarenta Quilos, while the most dissimilar were Branca 3 and Amarela da Rama Cinza. The divergence analysis indicated that promising crosses could be made between the Branca 3 cultivar and the Pão, Amarela 1, Fécula Branca and Amarela 2 cultivars for the high genetic divergence, favorable agronomic and culinary traits, and disease resistance on the part of at least one of the parents involved in the cross.