Abstract Chakhao is a popular pigmented black rice variety with remarkably high anthocyanin content. Due to its susceptibility to various biotic and abiotic stresses, its production has remained low. Here, two genes, Pi54 blast-resistant and OsSPL14, high-yielding genes of rice, were pyramided in a Chakhao landrace by crossing with CR Dhan 307. From a total of 147 F4 lines developed, 32 were identified with the positive alleles of both genes, of which 16 were black grain coloured, and their total anthocyanin content ranged from 30.19±2.19 to 240.31±2.62 mg 100 g-1 dried weight. Among these, ChM 68 F4 line had the highest anthocyanin content (240.31 ± 2.62 mg 100 g-1 of powdered grain) with more 118.96 mg 100 g-1 anthocyanin than the recipient parent, CHK13 (121.35±3.32 mg 100 g-1), making it a promising line to be released as a high-yielding and durable blast-resistant Chakhao variety in Manipur. stresses low Here Pi Pi5 blastresistant blast resistant OsSPL14 OsSPL highyielding yielding 307 14 F developed 3 1 coloured 3019219 30 19 2 30.19±2.1 24031262 240 31 62 240.31±2.6 10 g1 g g- weight these 6 240.31 24031 (240.3 262 2.6 11896 118 96 118.9 parent CHK CHK1 121.35±3.32 12135332 121 35 (121.35±3.3 g1, , g-1) Manipur OsSPL1 301921 30.19±2. 2403126 24 240.31±2. 240.3 2403 (240. 26 2. 1189 11 9 118. 121.35±3.3 1213533 12 (121.35±3. 30192 30.19±2 240312 240.31±2 240. (240 121.35±3. 121353 (121.35±3 3019 30.19± 240.31± (24 121.35±3 12135 (121.35± 301 30.19 (2 121.35± 1213 (121.35 30.1 ( 121.35 (121.3 30. 121.3 (121. 121. (121 (12 (1

Abstract The present study has been carried out with the seed extracts of Nyctanthes arbor-tristis L. (Parijat) and evaluates its antioxidant potential and profiling the phytochemical constituents by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The antioxidant potential of the seed extracts was measured by four different in vitro assay like 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, superoxide anion free radical scavenging activity, ferric reducing antioxidant power (FRAP) and lipid peroxidation inhibition potential (LPIP) assay. The total phenol content (TPC) and total flavonoid content (TFC) were estimated. The ethyl acetate extract (EAE) of seeds showed potential DPPH free radical scavenging activity (EC50 129.49±3.55µg/ml), superoxide anion radical (EC50 969.94±8.03µg/ml) and LPIP (EC50 452.43±5.07 µg/ml) activities. The total phenol content was maximum in aqueous extract (AQE) which was 201.00±0.20 µg/mg gallic acid equivalent. The EAE was rich with total flavonoid and it was found to be 34.50±0.40 µg/mg rutin equivalent. The EAE was subjected for phytochemical-profiling using GC-MS system. The presence of different phytoconstituents supports the medicinal value of the seeds. The results suggest that EAE constitutes a promising new source of novel compounds. Further, it can be used for isolation and purification of specific compounds which have good antioxidant activities and possess useful biological activities. arbortristis arbor tristis L Parijat (Parijat ChromatographyMass Chromatography Mass GCMS GC MS (GC-MS analysis 2 2diphenyl1picrylhydrazyl diphenylpicrylhydrazyl diphenyl 1 picrylhydrazyl (DPPH FRAP (FRAP (LPIP TPC (TPC TFC (TFC estimated (EAE EC50 EC (EC5 129.49±3.55µg/ml, 12949355µgml µgml 129.49±3.55µg/ml , 129 49 3 55µg ml µg 129.49±3.55µg/ml) 969.94±8.03µg/ml 96994803µgml 969 94 8 03µg 45243507 452 43 5 07 452.43±5.0 µg/ml AQE (AQE 20100020 201 00 0 20 201.00±0.2 µgmg mg equivalent 3450040 34 50 40 34.50±0.4 phytochemicalprofiling system Further EC5 (EC 12 4 96 9 4524350 45 452.43±5. 2010002 201.00±0. 345004 34.50±0. 452435 452.43±5 201000 201.00±0 34500 34.50±0 45243 452.43± 20100 201.00± 3450 34.50± 4524 452.43 2010 201.00 345 34.50 452.4 201.0 34.5 452. 201. 34.

ABSTRACT Aegle marmelos (L.) (Rutaceae) commonly known as bael is an important medicinal fruit tree. The present study focused on the effects of aqueous extract of Aegle marmelos (AEAM) on the testis and sperm characteristics induced by cyclophosphamide (CPA) in mice. Thirty six adult Parke’s strain mice were divided into six groups: group I given only distilled water (control); group II administered with AEAM alone once in a week for five weeks; group III administered with CPA (200 mg/kg b.w., intraperitoneally) once in a week for five weeks and group IV-VI CPA along with AEAM (400, 500 and 600 mg/kg b.w., orally). CPA was found to reduce gonadosomatic index (GSI), sperm counts, motility, viability, antioxidant activities and induced histopathological changes of testis. In the group administered AEAM with CPA an exacerbation of sperm count, motility and viability of the cauda epididymis, GSI, antioxidant activities and architecture of testis was observed. The results suggest that the administration of AEAM may aggravate CPA-induced reproductive toxicity. It may be helpful in preparation of natural male contraceptives.