Abstract The use of melatonin (MEL) on plants has recently become widespread. Melatonin (MEL) was used as elicitor in the leaf-derived sage (Salvia officinalis L.) callus culture, which allows production in a short time regardless of environmental factors. MEL was applied to the calli in various concentrations, and the effect on the amount and quality of phytochemicals was determined. MEL stimulated the production of the maximum quantity of sage calli and the synthesis of secondary metabolites when applied as an elicitor at a certain ratio (100 M). The callus induction rate decreased while the MEL concentration increased. Among the phytochemicals scanned by HPLC and GC-MS, especially the amount of rosmaniric acid was found to increase by 75% (100 µM). The amount of rosmarinic acid decreased gradually in the 200 µM and control groups. The color differences of the callus cultures were also considerable. The color, which was quite dark brown at 100 µM MEL, turned into a light color as the amount of rosmariniric acid decreased. In addition, phytochemicals such as α-thujone (27.56%), 1.8-cineole (5.9%), camphor (16.84%) analyzed in 100 µM MEL application have the highest rates compared to other applications. Phytochemicals present in the control group but not in the MEL treatments, and components present in the treatments but not in the control were detected (1.8-cineol, some aldehyde groups). Therefore, stimulating the production of pharmacologically valuable phytochemicals that can be obtained with a certain amount of MEL application in sage cell culture medium will provide an important commercial advantage. (MEL widespread leafderived leaf derived Salvia L. L factors concentrations determined (10 M. M . M) increased GCMS, GCMS GC MS, MS GC-MS 75 µM. µM) 20 groups considerable 10 addition αthujone α thujone 27.56%, 2756 27.56% , 27 56 (27.56%) 1.8cineole 18cineole cineole 1.8 1 8 5.9%, 59 5.9% 5 9 (5.9%) 16.84% 1684 16 84 (16.84% applications 1.8cineol, 18cineol cineol cineol, (1.8-cineol groups) Therefore advantage (1 7 2 275 27.56 (27.56% 8cineole 18 1. 5.9 (5.9% 16.84 168 (16.84 8cineol 1.8cineol ( 27.5 (27.56 5. (5.9 16.8 (16.8 27. (27.5 (5. 16. (16. (27. (5 (16 (27 (2

Abstract: Transverse joint spacing is one of the fundamental input parameters in structural design of jointed plain concrete pavement (JPCP). It has to be determined considering many factors to produce a well-performing and cost-effective JPCP. In this study, a comprehensive evaluation of transverse joint spacing in JPCP was carried out based on the followings: (1) guidelines based on previous studies, the Federal Highway Administration and the American Concrete Pavement Association recommendations were summarized; (2) empirical and mechanistic-empirical (M-E) pavement design methodologies were reviewed; (3) current state highway agency practices in USA were surveyed as part of this study and the survey results were documented; (4) effects of joint spacing on JPCP performance were evaluated based on field observations; and (5) lastly, an economic analysis was performed to evaluate effects of joint spacing on life-cycle costs. This study demonstrated that a joint spacing between 15-18 ft (4.6-5.5 m) seems to be provide a safe zone for both performance and cost effectiveness.

ABSTRACT The study was conducted to determine the effects of stocking density (SD, 5 or 7 layers/cage) and tarragon (Artemisia dracunculus L.) diet supplementation at four levels (0, 1.2, 6 and 12 g/kg feed) on performance, certain egg characteristics, serum, liver, egg yolk and small intestine bacteria parameters in laying hens. The experiment was carried out over a period of 8 weeks, with 192 Lohman Brown commercial hybrids at 50-w-age. The results showed that an increased SD reduced feed intake (FI; p<0.01) and egg production (p<0.05), but had no effect on the weight gain, feed conversion ratio, damaged egg ratio, egg weight and egg quality (p>0.05) parameters. The supplementation of tarragon to the diet reduced the FI and damaged egg ratio (p<0.01), and improved egg production and FCR (p<0.01). While an increased SD reduced serum total antioxidants (p<0.05), it elevated corticosterone (CORT) and total oxidant serum (TOS) (p<0.05). Tarragon was found to enhance total immunoglobulin (p<0.05), but to decreased the CORT and TOS of the serum (p<0.05). An increased SD raised the malondialdehyde (MDA) in the serum (p<0.001), liver (p<0.05) and yolk (p<0.001). Tarragon supplementation reduced MDA of the serum (p<0.05), liver (p<0.001) and yolk (p<0.001). E. coli and total Mesophilic Aerobic Bacteria counts in the small intestine were raised (p<0.001) with increased SD. Tarragon decreased (p<0.05) mesophilic aerobic bacteria. It was thus found that, tarragon supplementation can be considered generally effective in improving performance parameters, alleviating stress-induced negativities, reducing lipid peroxidation, regulating the immune system and controlling some intestinal microorganisms.

ABSTRACT Objective: The high-grade level of smear acid-resistant bacilli (ARB) positivity has been linked to increased infectiousness in pulmonary tuberculosis (TB). The ability to predict infectiousness is important in the management of the disease. The present study aimed to investigate the relationship between smear results, the clinical features, and the levels of radiological involvement of TB. Methods: A total of 245 cases diagnosed with pulmonary TB were admitted to the study. Data including age, sex, case definition, numbers and characteristics of symptoms, smear results, smear positivity grades, and levels of radiological involvement were recorded. Relations between smear results and other data were determined via cluster tree and regression analysis. Results: The group with only coughing had higher rates of both positive smear and high smear positivity levels (p = 0.014 and p = 0.02, respectively) compared to the group without coughing. Similarly, the groups with moderate or high radiological involvement showed significantly higher rates of both positive smear and high smear positivity level when compared to the group exhibiting low radiological involvement (p < 0.001). Conclusion: Patients with coughing and a moderate to high level of radiological involvement should be closely monitored due to their high-level risk of transmission.

ABSTRACT Objective: To determine the growth reference value of children in our province and to compare these values with current values of the USA and other local values of our country. Methods: A total of 615 boys and 586 girls with different socioeconomic levels and ages of 0–5 years were included in the study. In all children, weight, height, head circumference, abdominal circumference, hip circumference, mid-arm circumference, and lower segment length were taken. All measurements were performed by the same paediatrician. The lambda-mu-sigma method was used for the preparation of percentile curves. Results: The body weight, head circumference and chest circumference measurements of boys were significantly higher than those in girls. Anthropometric measurements of children in high-income groups were higher than those in the low-income group. All of the anthropometric parameters except upper mid-arm circumference were higher in children fed with breast milk and formula milk than in children who only fed with breast milk. The relation between the mother's education level and anthropometric parameters was insignificant. Conclusion: Our results showed that the anthropometric measurements of our children were similar to those of west Turkey as well as those of the United States. On the other hand, our children's anthropometric measurements were higher than other local values. Relatively high values of height have been interpreted as a genetic feature.

ABSTRACT The first aim of this study was to investigate the effects of perch cooling and different litter thicknesses on litter quality of the housing facility, welfare criteria (incidence of footpad and hock burns, gait score, plumage cleanliness and damages), heterophil/lymphocyte ratio, growth, and carcass compositions of broilers reared at high ambient temperature. Secondly, the study was conducted to determine whether cooled perches would be preferred by broiler chickens exposed to a hot environment, and subsequently, whether the utilization of these perches would improve the welfare and performance of broilers, beyond those provided by non-cooled perches. A total of 459 1-day-old male broiler chicks were randomly assigned to nine treatment groups based on the perch treatment (no perch, non-cooled, cooled perches) and litter thicknesses (1, 7, 14 cm) with three replicates. Aerial ammonia and litter moisture levels were lower in broilers reared at 14 cm litter thickness group than in broilers reared at 1 cm thickness group. The perch-contact incidence of chickens reared under the cooled perch was found higher than the non-cooled chickens at 36 to 42 days of age. Cooled perches and 14 cm of litter thickness tended to decrease the incidence of footpad and hock burn. The body weight gain of the broilers in cooled perch group was higher than those in no perch and non-cooled perch groups at 0-42 d (p<0.05). These results suggest that cool perches have a beneficial effect on the performance and welfare of broilers.

ABSTRACT This study investigated the effects of the addition of Lactobacillus reuteri E81 (LRE) into rations of chukar partridges (Alectoris chukar) fed under heat stress (HS) conditions on fattening performance and meat quality. This study included 256 chukar partridges aged 1 day. The study comprised an adaptation period of 7 days and a fattening period of 35 days and included 8 different groups with 32 animals in each group. Each group was further divided into four subgroups with eight animals in each subgroup. At the end of the study, the best results in terms of fattening performance in the non-HS groups were obtained in the LRE 600 ppm group and in the HS groups, SLRE 200 ppm had the best effect on average live weight and average live weight increase, whereas SLRE 400 ppm had the best effect on FCR (p<0.05). The analysis of the samples collected from chukar partridges on day 21 showed that, there was no effect on the colour parameters and Thiobarbituric acid reactive substances (TBARS) level in the LRE in the HS and non- HS groups, whereas the meat pH level decreased in the SLRE 400 ppm group (p<0.05). The analysis of the samples collected on day 42 showed that there was no effect on colour parameters in the HS and non-HS groups. TBARS level decreased at the dose of LRE 200 ppm in the non-HS group, and the meat pH level decreased in both HS and non-HS groups (p<0.05).

RESUMO Objetivo: A obesidade está associada a doenças oulares, mas as mudanças estruturais subjacentes e os mecanismos patogênicos não foram examinados detalhadamente. Aqui avaliamos os efeitos da obesidade mórbida nos índices morfométricos da doença ocular. Métodos: Voluntários obesos mórbidos (n=101, índice de massa corporal ³40) e indivíduos saudáveis (n=95, índice de massa corporal 18,50 a 24,99) foram examinados por tonometria de aplanação de Goldman, paquimetria e tomografia de coerência óptica de domício espectral. A pressão intraocular, profundidade da câmara anterior, comprimento axial, espessura central da córnea, espessura da camada de fibras nervosas da retina, espessura foveal central e espessura da coroide foram comparadas entre os grupos. Resultados: A pressão intraocular não corrigida foi significativamente maior no grupo com obesidade mórbida do que no grupo controle saudável (15,5 ± 2,5 vs. 14,5 ± 2,6 mmHg, p=0,009), enquanto que o comprimento axial, profundidade da câmara anterior e espessura central da córnea não diferiram entre os grupos. A espessura média da camada de fibras nervosas da retina no quadrante temporal foi reduzida no grupo com obesidade mórbida (72,7 ± 13,6 vs. 85,05 ± 52,6 mm, p=0,024). Da mesma forma, a média das espesuras da retinianas nas localizações nasal e temporal de 1500 m foi menor no grupo com obesidade mórbida (346,6 ± 18,2 mm vs. 353,7 ± 18,8 mm, p=0,008; 323,1 ± 20,3 mm vs. 330,0 ± 18,9 mm, p=0,001). A espessura média da coroide também foi reduzida em quase todos os locais de mensuração (fóvea, temporal 500 e 1000 mm, nasal 500, 1000 e 1500 mm) do grupo obeso (p<0,05). Peso e índice de massa corporal foram negativamente correlacionados com a espessura da coroide subfoveal (r=-0,186, p=0,009; r=-0,173, p=0,015). Conclusão: A obesidade mórbida está associada à elevada pressão intraocular não corrigida e a sinais de neuropatia e retinopatia. A obesidade pode, assim, aumentar os riscos de glaucoma e neuropatia óptica glaucomatosa.

ABSTRACT Purpose: Obesity is associated with eye diseases, but the underlying structural changes and pathogenic mechanisms have not been examined in detail. Here, we assessed the effects of morbid obesity on the morphometric indices of eye disease. Methods: Morbidly obese volunteers (n=101, body mass index [BMI] ³40) and healthy individuals (n=95, BMI: 18.50-24.99) were examined by Goldman applanation tonometry, pachymetry, and spectral domain optical coherence tomography. Intraocular pressure, anterior chamber depth, axial length, central corneal thickness, retinal nerve fiber layer thickness, central foveal thickness, and choroidal thickness were compared between groups. Results: Uncorrected intraocular pressure was significantly greater in the morbidly obese group than in the healthy control group (15.5 ± 2.5 vs. 14.5 ± 2.6 mmHg, p=0.009), whereas axial length, anterior chamber depth, and central corneal thickness did not differ between the groups. The mean retinal nerve fiber layer thickness at the temporal quadrant was reduced in the morbidly obese group (72.7 ± 13.6 vs. 85.05 ± 52.6 mm, p=0.024). Similarly, the mean retinal thicknesses at nasal and temporal 1500-mm locations were lower in the morbidly obese group (346.6 ± 18.2 vs. 353.7 ± 18.8 mm, p=0.008; 323.1 ± 20.3 vs. 330.0 ± 18.9 mm, p=0.001). The mean choroidal thickness was also reduced in almost all measurement locations (fovea, temporal 500 and 1000 mm, and nasal 500, 1000, and 1500 mm) of the obese group (p<0.05). Weight and BMI were negatively correlated with subfoveal choroidal thickness (r=-0.186, p=0.009; r=-0.173, p=0.015). Conclusion: Morbid obesity is associated with elevated uncorrected intraocular pressure and signs of neuropathy and retinopathy. Obesity may thus increase the risks of glaucoma and glaucomatous optic neuropathy.

ABSTRACT The present study was conducted to investigate the influence of different levels of dietary phytogenic feed additive (PFA) on growth performance, caecal microbiota, and intestinal morphology of broilers. A total of 480 Ross-308 one-day-old male broilers chicks (body weight 43±3 g) were randomly assigned to 32 replicate pens of four experimental groups, each experimental group consisting of 8 replicates (each replicate pen consisting of 15 chicks). A basal diet was formulated based on corn and soybean meal that was fed to the control group. Other dietary treatments received a commercial PFA at 100 mg/kg (PFA100), 125 mg/kg (PFA125), and 150 mg/kg (PFA150). Body weight gain, feed intake, and feed conversion rate of broilers were recorded on 1-21, 22-42, and 1-42 days of age. One bird was slaughtered on the 21st and 42nd days and caecal contents were aseptically collected. Jejunal tissue samples were also collected on the same days. Total aerobic bacteria, coliforms, Escherichia coli, and lactobacilli were counted in the caecal contents. Villus height, villus diameter, crypt depth, muscular thickness, and goblet cell number per villus were recorded. There was no difference among the dietary treatments for growth performance and caecal microbe populations at any phase. However, the dietary PFA supplementation increased the villus height, villus width, muscularis thickness, and reduced the crypt depth and goblet cell number per villus in broilers compared to those fed control diets. In conclusion, this study suggests that dietary supplementation of a PFA consisting of blend of different spices and essential oils did not improve growth performance and caecal microbial populations despite a positive improvement in the jejunal morphometry of broilers.

ABSTRACT Ewing's sarcoma is one of the most aggressive primary bone tumours. Ewing's sarcoma arising from the bones of the head and neck region is extremely rare; only 4—9% of all Ewing's sarcoma originate in this region. We report a case of Ewing's sarcoma localized in the mandible because of its unusual presentation.

RESUMEN El sarcoma de Ewing es uno de los tumores primarios óseos más agresivos. El sarcoma de Ewing de los huesos de la región de cabeza y cuello es extremadamente raro: sólo 4–9% de todos los sarcomas de Ewing se originan en esta región. Reportamos un caso de sarcoma de Ewing localizado en la mandíbula, debido a su inusual presentación.

Experiência: Objetivamos investigar os efeitos de metoclopramida e ondansetrona no bloqueio neuromuscular por mivacúrio. Métodos: Foram incluídos no estudo 75 pacientes ASA I-II, com idades entre 18 e 65 anos e agendados para cirurgia eletiva necessitando de intubação traqueal. Os pacientes receberam metoclopramida 10 mg, ondansetrona 4 mg ou salina normal 5 mL; grupo M, grupo O e grupo SN (n = 25) respectivamente. Antes da anestesia, os medicamentos em estudo foram administrados em um volume de 5 mL. O nível de colinesterase plasmática foram obtidos antes e 5 minutos depois da administração dos medicamentos em estudo e 5 minutos depois da administração de mivacúrio. Os tempos até o início e os níveis T25, T75, T25-75 e T90 foram comparados entre si, tendo sido investigadas as diferenças entre cada paciente. Depois de registrar T90, o estudo foi terminado, tendo início a cirurgia. Resultados: O tempo até o início foi significativamente mais breve no Grupo M versus os outros dois grupos. O tempo até o início no Grupo O foi significativamente mais breve versus grupo SN. No grupo M, T25, T75, T90 e os índices de recuperação foram significativamente maiores versus Grupo NS (p < 0,001). No Grupo O, T25 e T75 foram maiores versus Grupo NS (p < 0,01 e p < 0,05,respectivamente). No Grupo M, T75, T90 e índices de retorno da anestesia foram significativamente maiores versus Grupo O (p < 0,001, p < 0,01, p < 0,001, respectivamente). Nos Grupos M e O, os níveis plasmáticos de colinesterase diminuíram significativamente (p < 0,001). Depois da administração dos medicamentos em estudo e de mivacúrio. Houve também redução na colinesterase plasmática no Grupo NS 5 minutos após a administração de mivacúrio (p < 0,001). Conclusão: Consideramos que ondansetrona seja agente mais confiável do que metoclopramida, quando utilizada com mivacúrio.

Background: We aimed to investigate the effects of metoclopramide and ondansetrone on mivacurium neuromuscular blockade. Methods: Seventy five, ASA I-II patients, aged 18-65 and scheduled for elective surgery requiring tracheal intubation were included in the study. The patients received metoclopramide 10 mg, ondansetrone 4 mg or normal saline 5 mL; group M, group O, group NS (n = 25), respectively. Before anesthesia study drugs were administered in a volume of 5 mL. The level of plasma cholinesterase were obtained before and 5 minutes after the administration of study drugs and5 minutes after the administration of mivacurium. Onset time, T25, T75, T25-75, T90 levelswere compared with each other and differences between each patients were investigated. After recording T90, the study was terminated and surgery was started. Results: Onset time was significantly shorter in group M, than the other two groups. Onset time in group O was significantly shorter than in group NS. In Group M T25, T75, T90 and recovery indices were significantly greater than in Group NS (p < 0.001). In Group O T25, T75 were greater than Group NS (p < 0.01 and p < 0.05, respectively). In Group M T75, T90 and emergence indices were significantly higher than Group O (p < 0.001, p < 0.01, p < 0.001, respectively). In Groups M and O, plasma cholinesterase levels decreased significantly (p < 0.001) after administration of study drugs and mivacurium. Plasma cholinesterase also was reduced in Group NS 5 minutes after the administration of mivacurium (p < 0.001). Conclusion: Ondansetrone is believed to be more reliable agent than metoclopramide when used with mivacurium.