Resumo Avaliar o impacto de polimorfismos genéticos em interleucinas (IL1A rs17561, rs1304037; IL10 rs1800871; IL1RN rs9005), óxido nítrico (NOS2 rs2779249, rs2897518) e supressor da sinalização de citocinas (SOCS1 rs243327, rs33977706) na qualidade de vida relacionada à saúde bucal (QVRSB) de pacientes submetidos a tratamento endodôntico (TE). Métodos: A amostra foi composta por 108 participantes, que apresentavam dentes unirradiculares com lesão periapical assintomática. O impacto da QVRSB foi registrado usando o Oral Health Impact Profile (OHIP-14) antes, sete e 30 dias após o TE. Amostras de saliva foram coletadas como fonte de DNA genômico. Os polimorfismos genéticos foram genotipados por PCR em tempo real usando o método Taqman. Análises univariadas e multivariadas foram utilizadas (p<0,05). Resultados: Observou-se diferença significativa para o polimorfismo rs2297518 no gene NOS2 na limitação funcional nos modelos codominante (p=0,037) e recessivo (p=0,001); na dor física (p<0,001 em ambos os modelos); no desconforto psicológico (p<0,001 em ambos os modelos); na deficiência física (p<0,001 em ambos os modelos) e na deficiência psicológica (p<0,001 em ambos os modelos). Polimorfismos no gene SOCS1, no modelo recessivo, rs33977706 (p=0,045) e rs243327 (p=0,019), influenciaram a QVRSB no domínio desconforto psicológico. Conclusões: Polimorfismos nos genes NOS2 e SOCS1 influenciaram a QVRSB de pacientes submetidos a TE. IL1A ILA IL rs17561 rs rs1304037 IL1 rs1800871 ILRN RN rs9005, rs9005 , rs9005) NOS (NOS rs2779249 rs2897518 SOCS (SOCS (QVRSB TE . (TE) Métodos 10 participantes assintomática OHIP14 OHIP 14 (OHIP-14 antes 3 genômico Taqman p<0,05. p005 p p<0,05 0 05 (p<0,05) Resultados Observouse Observou se rs229751 p=0,037 p0037 037 (p=0,037 p=0,001 p0001 001 (p=0,001) p<0,001 (p<0,00 modelos. rs3397770 p=0,045 p0045 045 (p=0,045 rs24332 p=0,019, p0019 p=0,019 019 (p=0,019) Conclusões rs1756 rs130403 rs180087 rs900 rs277924 rs289751 (TE 1 OHIP1 (OHIP-1 p00 p<0,0 (p<0,05 rs22975 p=0,03 p003 03 (p=0,03 p=0,00 p000 00 (p=0,001 p<0,00 (p<0,0 rs339777 p=0,04 p004 04 (p=0,04 rs2433 p001 p=0,01 01 (p=0,019 rs175 rs13040 rs18008 rs90 rs27792 rs28975 (OHIP- p0 p<0, rs2297 p=0,0 (p=0,0 (p=0,00 (p<0, rs33977 rs243 (p=0,01 rs17 rs1304 rs1800 rs9 rs2779 rs2897 (OHIP p<0 rs229 p=0, (p=0, (p<0 rs3397 rs24 rs1 rs130 rs180 rs277 rs289 p< rs22 p=0 (p=0 (p< rs339 rs2 rs13 rs18 rs27 rs28 p= (p= (p rs33 rs3

Abstract To evaluate the impact of genetic polymorphisms in interleukins (IL1A rs17561, rs1304037; IL10 rs1800871; IL1RN rs9005), nitric oxide (NOS2 rs2779249, rs2897518) and suppressor of cytokine signaling (SOCS1 rs243327, rs33977706) on oral health-related quality of life (OHRQoL) of patients under-going root canal treatment (RCT). Methods: The sample consisted of 108 participants, presenting single-rooted teeth with asymptomatic periapical periodontitis. The impact of the OHRQoL was recorded using the Oral Health Impact Profile (OHIP-14) before, seven, and 30 days after RCT. Saliva samples were collected as a source of genomic DNA. Genetic polymorphisms were genotyped by Real-Time PCR using the Taqman method. Univariate and Multivariate analyses were used (p<0.05). Results: A significant difference was observed for the polymorphism rs2297518 in the NOS2 gene in functional limitation in the codominant (p=0.037) and recessive (p=0.001) models; in the physical pain (p<0.001 in both models); in psychological discomfort (p<0.001 in both models); in physical disability (p<0.001 in both models) and in psychological disability (p<0.001 in both models). Polymorphisms in the SOCS1 gene, in the recessive model, rs33977706 (p=0.045) and rs243327 (p=0.019), influenced the OHRQoL in the psychological discomfort domain. Conclusions: Polymorphisms in NOS2 and SOCS1 genes influenced the OHRQoL of patients undergoing RCT. IL1A ILA IL rs17561 rs rs1304037 IL1 rs1800871 ILRN RN rs9005, rs9005 , rs9005) NOS (NOS rs2779249 rs2897518 SOCS (SOCS healthrelated health related (OHRQoL under going RCT . (RCT) Methods 10 participants singlerooted single rooted periodontitis OHIP14 OHIP 14 (OHIP-14 before seven 3 DNA RealTime Real Time method p<0.05. p005 p p<0.05 0 05 (p<0.05) Results rs229751 p=0.037 p0037 037 (p=0.037 p=0.001 p0001 001 (p=0.001 models p<0.001 (p<0.00 models. model rs3397770 p=0.045 p0045 045 (p=0.045 rs24332 p=0.019, p0019 p=0.019 019 (p=0.019) domain Conclusions rs1756 rs130403 rs180087 rs900 rs277924 rs289751 (RCT 1 OHIP1 (OHIP-1 p00 p<0.0 (p<0.05 rs22975 p=0.03 p003 03 (p=0.03 p=0.00 p000 00 (p=0.00 p<0.00 (p<0.0 rs339777 p=0.04 p004 04 (p=0.04 rs2433 p001 p=0.01 01 (p=0.019 rs175 rs13040 rs18008 rs90 rs27792 rs28975 (OHIP- p0 p<0. rs2297 p=0.0 (p=0.0 (p<0. rs33977 rs243 (p=0.01 rs17 rs1304 rs1800 rs9 rs2779 rs2897 (OHIP p<0 rs229 p=0. (p=0. (p<0 rs3397 rs24 rs1 rs130 rs180 rs277 rs289 p< rs22 p=0 (p=0 (p< rs339 rs2 rs13 rs18 rs27 rs28 p= (p= (p rs33 rs3

Abstract The aim of this study was to evaluate whether polymorphisms in SOD2 and SOD3 genes modulate the oral health-related quality of life (OHRQoL) of Para athletes with dental caries experience. The cross-sectional study included 264 Para athletes (143 in athletics, 61 in weightlifting and 60 in swimming). A trained and calibrated team recorded the decayed, missing and filled teeth index (DMFT). The Brazilian version of the Oral Health Impact Profile (OHIP-14) was used to measure OHRQoL. Genomic DNA was extracted from the athletes’ saliva, and genetic polymorphisms in the SOD2 (rs5746136 and rs10370) and SOD3 (rs2855262 and rs13306703) genes were analyzed by real-time polymerase chain reaction. Univariate and multivariate analyses were performed. A multivariate General Linear Model analysis, adjusted for sex, revealed that the SOD3 gene polymorphism (rs2855262) had a significant effect on the psychological disability domain [codominant (p = 0.045) and recessive (p=0.038) models]. The SOD2 gene polymorphism (rs5746136) had a significant effect on the total OHIP-14 score [dominant model (p = 0.038)] and the psychological discomfort [dominant model (p = 0.034)] and physical disability [codominant model (p=0.037)] domains. Presence of the SOD2 rs10370 polymorphism led to statistical differences in the total score [codominant (p = 0.026) and dominant (p = 0.023) models] and the handicap domain scores [codominant (p = 0.027) and dominant (p = 0.032) models]. Polymorphisms of the SOD2 and SOD3 genes may be important biomarkers of OHRQoL in Para athletes with dental caries experience. SOD healthrelated health related (OHRQoL experience crosssectional cross sectional 26 143 (14 athletics 6 swimming. swimming . swimming) decayed DMFT. DMFT (DMFT) OHIP14 OHIP 14 (OHIP-14 saliva rs5746136 rs (rs574613 rs2855262 (rs285526 rs13306703 realtime real time reaction performed analysis sex codominant p 0.045 0045 0 045 p=0.038 p0038 038 (p=0.038 models. models OHIP-1 0.038 0038 0.038) 0.034 0034 034 0.034) p=0.037 p0037 037 (p=0.037) domains rs1037 0.026 0026 026 0.023 0023 023 0.027 0027 027 0.032 0032 032 2 (1 (DMFT OHIP1 1 (OHIP-1 rs574613 (rs57461 rs285526 (rs28552 rs1330670 0.04 004 04 p=0.03 p003 03 (p=0.03 OHIP- 0.03 003 (p=0.037 rs103 0.02 002 02 ( (OHIP- rs57461 (rs5746 rs28552 (rs2855 rs133067 0.0 00 p=0.0 p00 (p=0.0 rs10 (OHIP rs5746 (rs574 rs2855 (rs285 rs13306 0. p=0. p0 (p=0. rs1 rs574 (rs57 rs285 (rs28 rs1330 p=0 (p=0 rs57 (rs5 rs28 (rs2 rs133 p= (p= rs5 (rs rs2 rs13

Resumo Este estudo transversal teve como objetivo investigar a associação entre defeitos de desenvolvimento do esmalte (DDE) e polimorfismos de nucleotídeo único (SNPs) nos genes que codificam o receptor da vitamina D (VDR) e o hormônio da paratireoide (PTH). Pacientes ortodônticos em tratamento em uma escola Odontologia foram selecionados por amostragem de conveniência. Os DDEs foram avaliados e classificados por um examinador calibrado (Kappa>0,80) através de fotografias intraorais de acordo com os critérios propostos por Ghanim et al. (2015). Os tipos de DDE considerados para análise foram: hipoplasia de esmalte, hipomineralização molar-incisivo (HMI), hipomineralização de segundos molares decíduos (HSMD) e opacidades demarcadas não-HMI/HSMD. O DNA gnômico foi extraído de células bucais. Os SNPs em VDR (rs7975232) e PTH (rs694, rs6256 e rs307247) foram genotipados por PCR em tempo real. As análises estatísticas foram realizadas utilizando o software PLINK (versão 1.03, concebido por Shaun Purcell, EUA). Foram feitos teste de qui-quadrado e teste exato de Fisher com um nível de significância de 5%. Foram incluídos noventa e um (n=91) pacientes (49 do sexo feminino e 42 do sexo masculino) (idade média de 14,1±5,8 anos). A frequência de DDE foi de 38,5% (35 pacientes). As distribuições genotípicas estavam em equilíbrio de Hardy-Weinberg. Não foi encontrada associação estatisticamente significante entre os DDEs e os SNPs avaliados. Foi observada uma associação limítrofe (p=0,09) entre a DDE e o haplótipo CC para o SNP rs7975232 no VDR. Em conclusão, os SNPs seleccionados nos genes VDR e PTH não foram associados à DDE nas amostras estudadas. (DDE (SNPs (VDR PTH. . (PTH) conveniência Kappa>0,80 Kappa080 Kappa 0 80 (Kappa>0,80 al 2015. 2015 (2015) molarincisivo molar incisivo HMI, HMI , (HMI) HSMD (HSMD nãoHMI/HSMD. nãoHMIHSMD HMI/HSMD. não-HMI/HSMD bucais rs (rs7975232 rs694, rs694 (rs694 rs625 rs307247 real versão 103 1 03 1.03 Purcell EUA. EUA EUA) quiquadrado qui quadrado 5 5% n=91 n91 n 91 (n=91 49 (4 4 masculino idade 14158 14 8 14,1±5, anos. anos anos) 385 38 38,5 35 (3 pacientes. pacientes) HardyWeinberg. HardyWeinberg Hardy Weinberg. Weinberg Hardy-Weinberg p=0,09 p009 p 09 (p=0,09 rs797523 conclusão estudadas (PTH Kappa>0,8 Kappa08 (Kappa>0,8 201 (2015 (HMI nãoHMI nãoHMI/HSMD HMIHSMD HMI/HSMD (rs797523 rs69 (rs69 rs62 rs30724 10 1.0 n=9 n9 9 (n=9 ( 1415 14,1±5 3 38, p=0,0 p00 (p=0,0 rs79752 Kappa>0, Kappa0 (Kappa>0, 20 (201 (rs79752 rs6 (rs6 rs3072 1. n= (n= 141 14,1± p=0, p0 (p=0, rs7975 Kappa>0 (Kappa>0 2 (20 (rs7975 (rs rs307 (n 14,1 p=0 (p=0 rs797 Kappa> (Kappa> (2 (rs797 rs30 14, p= (p= rs79 (Kappa (rs79 rs3 (p rs7 (rs7

Abstract This cross-sectional study aimed to investigate the association between developmental defects of enamel (DDE) and single nucleotide polymorphisms (SNPs) in the genes encoding the vitamin D receptor (VDR) and parathyroid hormone (PTH). Orthodontic patients receiving treatment at a dental school were selected through convenience sampling. Intra-oral photographs were used to assess DDE, which were classified according to the criteria proposed by Ghanim et al. (2015) by a single calibrated examiner (Kappa>0.80). Enamel hypoplasia, molar-incisor hypomineralization (MIH), hypomimineralized second primary molar (HSPM), and non-MIH/HSPM demarcated opacities were considered for the analysis. Genomic DNA was extracted from buccal cells. The SNPs in VDR (rs7975232) and PHT (rs694, rs6256, and rs307247) were genotyped using real-time polymerase chain reactions (PCR). Statistical analyses were performed using the PLINK software (version 1.03, designed by Shaun Purcell, EUA). Chi-square or Fisher's exact tests were performed at a significance level of 5%. Ninety-one (n=91) patients (49 females and 42 males) (mean age of 14.1±5.8 years) were included. The frequency of DDE was 38.5% (35 patients). Genotype distributions were in Hardy-Weinberg equilibrium. No significant statistical association was found between DDE and the SNPs evaluated. A borderline association (p=0.09) was observed between DDE and the CC haplotype for SNP rs7975232 in VDR. In conclusion, the selected SNPs in VDR and PTH genes were not associated with DDE in the studied samples. crosssectional cross sectional (DDE (SNPs (VDR PTH. . (PTH) sampling Intraoral Intra oral al 2015 (2015 Kappa>0.80. Kappa080 Kappa Kappa>0.80 0 80 (Kappa>0.80) hypoplasia molarincisor incisor MIH, MIH , (MIH) HSPM, HSPM (HSPM) nonMIH/HSPM nonMIHHSPM non MIH/HSPM analysis cells rs (rs7975232 rs694, rs694 (rs694 rs6256 rs307247 realtime real time PCR. PCR (PCR) version 103 1 03 1.03 Purcell EUA. EUA EUA) Chisquare Chi square Fishers Fisher s 5 5% Ninetyone Ninety one n=91 n91 n 91 (n=91 49 (4 4 males mean 14158 14 8 14.1±5. years included 385 38 38.5 35 (3 patients. patients) HardyWeinberg Hardy Weinberg equilibrium evaluated p=0.09 p009 p 09 (p=0.09 rs797523 conclusion samples (PTH 201 (201 Kappa08 Kappa>0.8 (Kappa>0.80 (MIH (HSPM nonMIH MIHHSPM (rs797523 rs69 (rs69 rs625 rs30724 (PCR 10 1.0 n=9 n9 9 (n=9 ( 1415 14.1±5 3 38. p=0.0 p00 (p=0.0 rs79752 20 (20 Kappa0 Kappa>0. (Kappa>0.8 (rs79752 rs6 (rs6 rs62 rs3072 1. n= (n= 141 14.1± p=0. p0 (p=0. rs7975 2 (2 Kappa>0 (Kappa>0. (rs7975 (rs rs307 (n 14.1 p=0 (p=0 rs797 Kappa> (Kappa>0 (rs797 rs30 14. p= (p= rs79 (Kappa> (rs79 rs3 (p rs7 (Kappa (rs7

Resumo O presente estudo teve como objetivo explorar a associação entre o padrão esquelético craniofacial sagital e a dimensão do seio frontal em adolescentes alemães. Tratou-se de um estudo transversal que incluiu pacientes ortodônticos alemães com idade variando entre 11 e 18 anos (80 homens, 82 mulheres). Cefalogramas laterais foram utilizados na análise cefalométrica e do seio frontal. A análise cefalométrica foi realizada para mensuração dos ângulos SNA, SNB e ANB. Medidas do seio frontal foram realizadas para obtenção da altura e dimensão ântero-posterior do seio frontal. A área da projeção lateral do seio frontal também foi determinada. As dimensões do seio frontal foram comparadas entre os sexos pelo teste de Mann Withney. Teste de Kruskal Wallis e pós teste de Dunn foram utilizados para comparar as diferenças entre as más oclusões esqueléticas. Também foram realizados o teste de correlação de Spearman e um modelo de regressão linear. A significância estatística foi estabelecida em 5%. Quanto à má oclusão esquelética, 71 pacientes apresentavam classe I, 81 classe II e 10 classe III. A altura (p = 0,009), dimensão ântero-posterior (p = 0,001) e área da projeção lateral do seio frontal (p = 0,007) foram maiores nos homens do que nas mulheres. A dimensão ântero-posterior do seio frontal foi significativamente maior na classe III quando comparada à classe I (p = 0,010) e classe II (p = 0,027). A área da projeção lateral do seio frontal também foi maior na classe III do que na classe I (p = 0,039). No modelo de regressão linear foi observada associação significativa apenas entre a área da projeção lateral do seio frontal e a classe III. Nossos resultados sugerem que a área da projeção lateral do seio frontal pode presentar diferenças morfométricas em adolescentes com maloclusão esquelética Classe III. Tratouse Tratou se 1 80 (8 8 mulheres . mulheres) SNA ANB ânteroposterior ântero posterior determinada Withney esqueléticas 5 5% 7 p 0,009, 0009 0,009 , 0 009 0,009) 0,001 0001 001 0,007 0007 007 0,010 0010 010 0,027. 0027 0,027 027 0,027) 0,039. 0039 0,039 039 0,039) ( 000 0,00 00 0,01 01 002 0,02 02 003 0,03 03 0,0 0,

Abstract The present study explored the association between the craniofacial sagittal skeletal patterns and frontal sinus dimensions in Germans. This cross-sectional study included orthodontic patients with age ranging from 11 to 18 (80 males, 82 females). Lateral cephalograms were used in the cephalometric and frontal sinus analysis. Cephalometric analysis was performed to measure SNA, SNB and ANB angles. Frontal sinus measurements were performed to obtain the frontal sinus height, antero-posterior dimension and frontal sinus lateral projection area. The frontal sinus dimensions were compared between genders with Mann Withney test. Kruskal Wallis and Dunn's post-hoc tests were used to compare the mean differences between the skeletal malocclusions. Spearman’s correlation test and linear regression model were also performed. Statistical significance was set at 5%. Regarding skeletal malocclusion, 71 patients presented class I, 81 class II and 10 class III skeletal malocclusion. The frontal sinus height (p=0.009), frontal sinus antero-posterior dimension (p=0.001) and frontal sinus lateral projection area (p=0.007) were bigger in males than in females. The frontal sinus antero-posterior dimension was significantly greater in the class III when compared to the class I (p=0.010) and class II (p=0.027). Frontal sinus lateral projection area was also bigger in class III than class I (p=0.039). In the linear regression model a significant association was observed between frontal sinus lateral projection area and class III (skeletal class I as a reference). In conclusion, our result suggests that the frontal sinus lateral projection area might present morphometric differences in German teenagers with skeletal class III malocclusion. Germans crosssectional cross sectional 1 80 (8 8 females . females) SNA angles anteroposterior antero posterior Dunns Dunn s posthoc post hoc malocclusions Spearmans Spearman 5 5% malocclusion 7 p=0.009, p0009 p p=0.009 , 0 009 (p=0.009) p=0.001 p0001 001 (p=0.001 p=0.007 p0007 007 (p=0.007 p=0.010 p0010 010 (p=0.010 p=0.027. p0027 p=0.027 027 (p=0.027) p=0.039. p0039 p=0.039 039 (p=0.039) reference. reference reference) conclusion ( p000 p=0.00 00 (p=0.009 (p=0.00 p=0.01 p001 01 (p=0.01 p002 p=0.02 02 (p=0.027 p003 p=0.03 03 (p=0.039 p00 p=0.0 (p=0.0 (p=0.02 (p=0.03 p0 p=0. (p=0. p=0 (p=0 p= (p= (p

Resumo A autofagia é um mecanismo de autodigestão das células, que está relacionado ao estresse celular. Ela permite a sobrevivência das células, mantendo a homeostase celular ou iniciando a morte celular. O objetivo deste estudo foi investigar a sinalização intracelular da regulação da autofagia induzida por pressão nas células do ligamento periodontal humano (PDL) e analisar o envolvimento de Rho kinases (ROCK) e espécies reativas de oxigênio (ROS) em particular. As células do ligamento periodontal humano foram tratadas com o inibidor de ROCK Y-27632 e o eliminador de ROS N-acetilcisteína (NAC) em combinação com magnitudes de pressão de 2, 6 e 8 g/cm2 durante 16 horas. As células tratadas com rapamicina serviram como controle positivo e as células não tratadas como grupo de controle. O kit de detecção de autofagia Cyto-ID® foi usado para análise de citometria de fluxo. A análise estatística foi realizada usando ANOVA e testes post-hoc. Os resultados mostram que a autofagia induzida por pressão foi afetada de forma diferente pelos dois inibidores (p<0,05). A aplicação do Y-27632 levou a uma redução significativa da autofagia em todos os grupos de pressão. A aplicação de NAC levou à redução da autofagia nas pressões de 2 g/cm2 e 6 g/cm2. A 8 g/cm2, esse efeito não estava mais presente. No grupo de controle, a autofagia foi significativamente reduzida pelo Y-27632 e significativamente aumentada pela NAC. Nossos dados sugerem que tanto a Rho-kinase quanto as espécies reativas de oxigênio podem influenciar a regulação da autofagia induzida pela pressão nas células PDL. PDL (PDL (ROCK (ROS particular Y27632 Y 27632 Y-2763 Nacetilcisteína N acetilcisteína (NAC gcm2 gcm g cm2 cm g/cm 1 horas CytoID® CytoID Cyto ID® ID Cyto-ID fluxo posthoc. posthoc post hoc. hoc post-hoc p<0,05. p005 p p<0,05 . 0 05 (p<0,05) presente Rhokinase kinase Y2763 2763 Y-276 p00 p<0,0 (p<0,05 Y276 276 Y-27 p0 p<0, (p<0,0 Y27 27 Y-2 p<0 (p<0, Y2 Y- p< (p<0 (p< (p

Abstract Autophagy is a self-digestion mechanism of cells, which is related to cell stress. It enables cell survival by maintaining cellular homeostasis or initiates cell death. This study aimed to investigate the intracellular signaling of pressure-induced autophagy regulation in human periodontal ligament (PDL) cells and to analyze the involvement of Rho kinases (ROCK) and reactive oxygen species (ROS) in particular. Human PDL cells were treated with the ROCK inhibitor Y-27632 and the ROS scavenger N-acetylcysteine (NAC) in combination with pressure magnitudes of 2, 6, and 8 g/cm2 over 16 hours. Cells treated with rapamycin served as a positive control and untreated cells as a control group. The Cyto-ID® Autophagy Detection Kit was used for flow cytometric analysis. Statistical analysis was performed using ANOVA and post-hoc tests. The results show that the pressure-induced autophagy was affected differently by the two inhibitors (p<0.05). The application of Y-27632 led to a significant reduction in autophagy in all pressure groups. The application of NAC led to reduced autophagy at pressures of 2 g/cm2 and 6 g/cm2. At 8 g/cm2, this effect was no longer present. In the control group, autophagy was significantly reduced by Y-27632 and significantly increased by NAC. Our data suggest that both Rho-kinase and reactive oxygen species could influence pressure-induced autophagy regulation in PDL cells. selfdigestion self digestion stress death pressureinduced induced (PDL (ROCK (ROS particular Y27632 Y 27632 Y-2763 Nacetylcysteine N acetylcysteine (NAC gcm2 gcm g cm2 cm g/cm 1 hours group CytoID® CytoID Cyto ID® ID Cyto-ID posthoc post hoc tests p<0.05. p005 p p<0.05 . 0 05 (p<0.05) groups present Rhokinase kinase Y2763 2763 Y-276 p00 p<0.0 (p<0.05 Y276 276 Y-27 p0 p<0. (p<0.0 Y27 27 Y-2 p<0 (p<0. Y2 Y- p< (p<0 (p< (p

Abstract This study aimed to assess whether genetic polymorphisms in MTR and MTRR are potential biomarkers of oral health-related quality of life (OHRQoL) in children with caries. A cross-sectional study was designed wherein pairs of parents/caregivers and children (aged two–five years) were selected. Clinical examination was used to detect dental caries, which were classified as low-severity and high-severity caries. The Early Childhood Oral Health Impact Scale (ECOHIS) questionnaire was used to assess OHRQoL. Genomic DNA extracted from the saliva was used to analyze two missense genetic polymorphisms: MTR (rs1805087) and MTRR (rs1801394). Mann–Whitney non-parametric test was used to analyze candidate genes with OHRQoL scale and domain, with a significance level of p≤0.05. MTR (rs1805087) was found associated (p = 0.05) with children’s OHRQoL subscale scores in the dominant model (GG + AG). Genetic polymorphisms in MTR may increase the risk of poor OHRQoL in children with caries. Further studies are needed to investigate genetics, molecular factors, and OHRQoL. healthrelated health related (OHRQoL caries crosssectional cross sectional parentscaregivers parents caregivers aged twofive five years selected lowseverity low severity highseverity high ECOHIS (ECOHIS rs1805087 rs (rs1805087 rs1801394. rs1801394 . (rs1801394) MannWhitney Mann Whitney nonparametric non parametric domain p005 p 0 05 p≤0.05 0.05 005 childrens s GG AG. AG AG) genetics factors rs180508 (rs180508 rs180139 (rs1801394 p00 p≤0.0 0.0 00 rs18050 (rs18050 rs18013 (rs180139 p0 p≤0. 0. rs1805 (rs1805 rs1801 (rs18013 p≤0 rs180 (rs180 (rs1801 p≤ rs18 (rs18 rs1 (rs1 (rs

Abstract Objective Many genes and signaling molecules are involved in orthodontic tooth movement, with mechanically and hypoxically stabilized HIF-1α having been shown to play a decisive role in periodontal ligament signaling during orthodontic tooth movement. Thus, this in vitro study aimed to investigate if genetic polymorphisms in HIF1A (Hypoxia-inducible factor α-subunits) influence the expression pattern of HIF-1α protein during simulated orthodontic compressive pressure. Methodology Samples from human periodontal ligament fibroblasts were used and their DNA was genotyped using real time Polymerase chain reaction for the genetic polymorphisms rs2301113 and rs2057482 in HIF1A . For cell culture and protein expression experiments, six human periodontal ligament fibroblast cell lines were selected based on the patients’ genotype. To simulate orthodontic compressive pressure in fibroblasts, a 2 g/cm2 force was applied under cell culture conditions for 48 hours. Protein expression was evaluated by Western Blot. Paired t-tests were used to compare HIF-1α expression with and without compressive pressure application and unpaired t-tests were used to compare expression between the genotypes in rs2057482 and rs2301113 (p<0.05). Results The expression of HIF-1α protein was significantly enhanced by compressive pressure application regardless of the genotype (p<0.0001). The genotypes in the genetic polymorphisms rs2301113 and rs2057482 were not associated with HIF-1α protein expression (p>0.05). Conclusions Our study confirms that compressive pressure application enhances HIF-1α protein expression. We could not prove that the genetic polymorphisms in HIF1A affect HIF-1α protein expression by periodontal ligament fibroblasts during simulated orthodontic compressive force. movement HIF1α HIFα HIF 1α α Thus HIFA A Hypoxiainducible Hypoxia inducible αsubunits subunits α-subunits rs rs230111 rs205748 experiments patients gcm2 gcm g cm2 cm g/cm 4 hours Blot ttests t tests p<0.05. p005 p p<0.05 0 05 (p<0.05) p<0.0001. p00001 p<0.0001 0001 (p<0.0001) p>0.05. p>0.05 (p>0.05) rs23011 rs20574 p00 p<0.0 (p<0.05 p0000 p<0.000 000 (p<0.0001 p>0.0 (p>0.05 rs2301 rs2057 p0 p<0. (p<0.0 p000 p<0.00 00 (p<0.000 p>0. (p>0.0 rs230 rs205 p<0 (p<0. (p<0.00 p>0 (p>0. rs23 rs20 p< (p<0 p> (p>0 rs2 (p< (p> (p

Abstract An increasing number of systematic reviews (SR) has investigated the association between dental caries and nutritional status in children and adolescents, thus requiring an overview to compile the information in a single piece of evidence. Therefore, this study aimed to evaluate and summarize evidence from published SR on the association between dental caries and nutritional status in children and adolescents. A wide search was conducted on May 29, 2023, in six databases (Medline via PubMed, Scopus, Web of Science, Cochrane library, Embase, and the Virtual Health Library – VHL). An additional search was performed in the gray literature (Open grey and Google Scholar), SR registration databases, and the list of references of the included SR. Our inclusion criteria were based on acronym PECOS. Overall, two reviewers independently extracted the data, evaluated the risk of bias (ROBIS), and assessed the quality of the chosen studies (AMSTAR-2). Data from the included meta-analysis were summarized and certainty of evidence using the GRADE approach was performed. After removing duplicates and applying our eligibility criteria, 19 SR from 2006-2022 were included. We found that 17 SR showed high risk of bias and critically low methodological quality. We observed an association between dental caries experiences and nutritional status since seven SR found an association between obesity/overweight and dental caries; one, an association between underweight and dental caries; and eleven, no associations. The meta-analysis showed divergent results according to the study designs, used indices, and participants’ age group, and were scored as having a very low certainty of evidence. Therefore, based on the high risk of bias, low methodological quality, and very low certainty of evidence of the chosen SR, most studies found no association between children and adolescents’ nutritional status and dental caries experience. (SR adolescents Therefore 29 2023 Medline PubMed Scopus Science library Embase VHL. VHL . VHL) Open Scholar, Scholar , Scholar) PECOS Overall data ROBIS, ROBIS (ROBIS) AMSTAR2. AMSTAR2 AMSTAR 2 (AMSTAR-2) metaanalysis meta analysis 1 20062022 2006 2022 2006-202 obesityoverweight obesity overweight one eleven associations designs indices participants group experience 202 (ROBIS (AMSTAR-2 2006202 200 2006-20 20 (AMSTAR- 200620 2006-2 (AMSTAR 20062 2006-

Abstract Background Genetic polymorphisms have been shown to influence several physiological traits, including dental and craniofacial characteristics. Understanding the clinical relevance of genetic polymorphisms in dental practice is crucial to personalize treatment plans and improve treatment outcomes. Objective to evaluate the association between dental age and genetic polymorphisms in genes encoding estrogen receptors alpha and beta (ESR1 and ESR2, respectively) in a sample of Brazilian children. Methodology This retrospective cross-sectional study was performed with children undergoing orthodontic treatment. Patients with syndromes, congenital anomalies, craniofacial deformities, under hormonal or systemic treatment, and with a previous history of facial trauma were excluded. Panoramic radiographs were used to assess dental age according to the Demirjian, Goldstein, and Tanner method. A delta [dental age-chronological age (DA-CA)] was obtained, which shows whether the patient tends to have a normal, delayed (negative values), or advanced (positive values) dental age. DNA isolated from buccal cells was used to genotype four genetic polymorphisms: rs9340799 (A>G) and rs2234693 (C>T), located in ESR1; and rs1256049 (C>T) and rs4986938 (C>T), located in ESR2. A statistical analysis was performed and values of p<0.05 indicated statistical difference. Results A total of 79 patients were included, 44 (55.70%) girls and 35 (44.30%) boys. The Demirjian, Goldstein, and Tanner method, in general, overestimated patients’ age by 0.75 years. There was no difference in the delta of dental age between the sexes (p>0.05). Genetic polymorphisms in ESR1 and ESR2 were not associated with dental age (p>0.05). Conclusion The studied genetic polymorphisms in ESR1 and ESR2 were not associated with dental age in Brazilian children. traits characteristics outcomes ESR (ESR respectively crosssectional cross sectional syndromes anomalies deformities excluded Demirjian Goldstein method agechronological chronological DACA DA CA (DA-CA) obtained normal negative values, , positive rs rs934079 A>G AG G (A>G rs223469 C>T, CT C>T C T rs125604 (C>T rs498693 p005 p 0 05 p<0.0 7 included 4 55.70% 5570 55 70 (55.70% 3 44.30% 4430 30 (44.30% boys general 075 75 0.7 years p>0.05. p>0.05 . (p>0.05) (DA-CA rs93407 rs22346 rs12560 rs49869 p00 p<0. 55.70 557 5 (55.70 44.30 443 (44.30 07 0. p>0.0 (p>0.05 rs9340 rs2234 rs1256 rs4986 p0 p<0 55.7 (55.7 44.3 (44.3 p>0. (p>0.0 rs934 rs223 rs125 rs498 p< 55. (55. 44. (44. p>0 (p>0. rs93 rs22 rs12 rs49 (55 (44 p> (p>0 rs9 rs2 rs1 rs4 (5 (4 (p> ( (p

ABSTRACT Objective: To investigate the association between single nucleotide polymorphisms in the COX2 gene (rs689466 and rs5275) and local and systemic signs and symptoms of teething. Material and Methods: Forty-four pairs of mothers-babies/toddlers were included. Erupted primary teeth were evaluated during clinical examination. Local and systemic signs and symptoms of teething were obtained from mothers' reporting via anamnesis. Samples of buccal cells were retrieved for DNA genotyping using real-time PCR. The T-test, Chi-square test, logistic regression, and haplotype analyses were applied. Results: Almost all mothers (95.5%) reported at least one local or systemic sign and symptom of teething. The most common was increased salivation (79.5%), diarrhea (72.3 %), and fever (70.5 %). The mean number of signs and symptoms per child was higher in boys than girls (mean = 5.1; SD= 1.5; p=0.008). Sleep disturbance (p=0.03) and loss of appetite (p=0.05) were more reported in boys. The rs689466 and rs5275 were not associated with signs and symptoms of teething (p>0.05). Conclusion: The single nucleotide polymorphisms in the COX2 gene (rs689466 and rs5275) were not associated with local and systemic signs and symptoms of teething. Objective COX rs (rs68946 Methods Fortyfour Forty four mothersbabies/toddlers mothersbabiestoddlers babies/toddlers babies toddlers included examination anamnesis realtime real time PCR Ttest, Ttest T test T-test Chisquare Chi square regression applied Results 95.5% 955 95 5 (95.5% 79.5%, 795 79.5% , 79 (79.5%) 72.3 723 72 3 (72. %, % %) 70.5 705 70 (70. %. . 5.1 51 1 SD 1.5 15 p=0.008. p0008 p p=0.008 0 008 p=0.008) p=0.03 p003 03 (p=0.03 p=0.05 p005 05 (p=0.05 rs68946 rs527 p>0.05. p>0.05 (p>0.05) Conclusion (rs6894 mothersbabies babiestoddlers 95.5 9 (95.5 79.5 7 (79.5% 72. (72 70. (70 5. 1. p000 p=0.00 00 p=0.0 p00 (p=0.0 rs6894 rs52 p>0.0 (p>0.05 (rs689 95. (95. 79. (79.5 (7 p=0. p0 (p=0. rs689 rs5 p>0. (p>0.0 (rs68 (95 (79. ( p=0 (p=0 rs68 p>0 (p>0. (rs6 (9 (79 p= (p= rs6 p> (p>0 (rs (p (p>

Resumo O objetivo do estudo foi explorar a influência de polimorfismos genéticos em ANKK1 e DRD2 sobre os sinais e sintomas da disfunção temporomandibular (DTM) em trabalhadores da construção civil. Este estudo transversal incluiu apenas indivíduos do sexo masculino. Todos os trabalhadores da construção civil eram saudáveis e maiores de 18 anos. Foram excluídos os trabalhadores analfabetos e analfabetos funcionais. O diagnóstico de DTM foi estabelecido de acordo com o Research Diagnostic Criteria para DTM (RDC/TMD). O DNA genômico foi usado para avaliar os polimorfismos genéticos ANKK1 (rs1800497) e DRD2 (rs6275; rs6276) usando PCR em tempo real. Testes qui-quadrado ou exato de Fisher foram utilizados para avaliar genótipos e distribuição de alelos entre os fenótipos estudados. O alfa estabelecido deste estudo foi de 5%. A amostra incluiu um total de 115 pacientes. A idade dos pacientes variou de 19 a 70 anos (média de idade 38,2; desvio padrão 11,7). Dor crônica (87,7%), deslocamento de disco (38,2%) e inflamação articular (26,9%) foram os sinais e sintomas mais observados. O polimorfismo genético rs6276 em DRD2 foi associado a dor crônica (p=0,033). Em conclusão, nosso estudo sugere que polimorfismos genéticos em DRD2 e ANKK1 podem influenciar sinais e sintomas de DTM em um grupo de trabalhadores da construção civil do sexo masculino.

Abstract The study aimed to explore the influence of genetic polymorphisms in ANKK1 and DRD2 on the signs and symptoms of temporomandibular disorder (TMD) in construction workers. This cross-sectional study included only male subjects. All construction workers were healthy and over 18 years age. Illiterate workers and functionally illiterate workers were excluded. The diagnosis of TMD was established according to the Research Diagnostic Criteria for TMD (RDC/TMD). Genomic DNA was used to evaluate the genetic polymorphisms ANKK1 (rs1800497) and DRD2 (rs6275; rs6276) using Real-Time PCR. Chi-square or Fisher exact tests were used to evaluate genotypes and allele distribution among the studied phenotypes. The established alpha of this study was 5%. The sample included a total of 115 patients. The age of the patients ranged from 19 to 70 years (mean age 38.2; standard deviation 11.7). Chronic pain (87.7%), disc displacement (38.2%), and joint inflammation (26.9%) were the most frequently observed signs and symptoms. The genetic polymorphism rs6276 in DRD2 was associated with chronic pain (p=0.033). In conclusion, our study suggests that genetic polymorphisms in DRD2 and ANKK1 may influence TMD signs and symptoms in a group of male construction workers.

Resumo Considerando que a fumaça de cigarro é um problema de saúde pública que está crescendo entre os adolescentes, o objetivo deste estudo foi investigar o impacto da fumaça de cigarro na sinalização osteogênica e osteoclastogênica da sutura palatina mediana de ratos. Foram utilizados ratos Wistar machos expostos (n=30) e não expostos à fumaça de cigarro (n=30). A exposição à fumaça de cigarro foi realizada duas vezes ao dia por 3 minutos, com um intervalo de 12 horas entre as exposições. Os animais foram mortos após o período experimental de 3, 7, 14 e 21 dias. Os tecidos coletados foram analisados em microscópico de luz e pelo RT-PCR em tempo real foi realizado para investigar a expressão gênica. s dados obtidos foram comparados usando o testes de Kruskal Wallis e Dunn (⍺ = 5%). Morfoligicamente, não houve mudança significativa na sutura palatina mediana nos ratos expostos ou não à fumaça de cigarro durante os tempo de 3, 7, 14 e 21 dias (p> 0.05). Por outro lado, a sinalização osteogênica esta aumentada nos animais expostos à fumaça e foi caracterizado por um aumento da produção de RANKL aos 3 e 14 dias (p <0.05), sem mudança na síntese da produção de RANK e osteoprotegerina (p> 0.05). Curiosamente, nos animais expostos, também foi observado um aumento precoce da síntese de osteocalcina, sialoproteína óssea e de osteopontina aos 3 dias de exposição, o que não foi mantido ao longo do tempo. A fumaça de cigarro modula a sinalização osteogênica e osteoclastogênica na sutura palatina mediana de ratos jovens, apesar de não tenha sido evidenciado alterações morfológicas.

Abstract Considering that smoking is a public health problem that has been growing among adolescents, the aim of this study was to investigate the impact of cigarette smoke on osteogenic and osteoclastogenic signaling in middle palatal suture of rats. Male Wistar rats exposed (n = 30) or not to cigarette smoke (n = 30) were used. Exposure to smoke was carried out for two daily periods of 3 minutes each, with an interval of 12 hours between exposures. After the experimental periods of 3, 7, 14 and 21 days, the animals were euthanized. The collected tissues were analyzed using light microscopy and real-time RT-PCR was performed to investigate gene expression. The data obtained were compared using the Kruskal Wallis and Dunn tests (⍺ = 5%). Morphologically, there were no significant changes in the middle palatal suture of rats exposed or not to cigarette smoke during 3, 7, 14 and 21 days (p> 0.05). On the other hand, osteoclastogenic signaling was increased in animals exposed to smoke and was characterized by a higher production of RANKL at 3 and 14 days (p <0.05), with no change in the synthesis of RANK and osteoprotegerin (p> 0.05). Interestingly, in the exposed animals, an early increase in the synthesis of osteocalcin, bone sialoprotein and osteopontin was also identified at 3 days of exposure (p <0.05), not sustained over time (p> 0.05). Cigarette smoke modulates osteogenic and osteoclastogenic signaling in the middle palatal suture of young rats, although morphological changes have not been evidenced.

Abstract: This cross-sectional study aimed to assess if genetic polymorphisms in TNF- α are associated with a negative impact on Oral Health-Related Quality of Life (OHRQoL) in children with dental caries. A total of 307 pairs of parents/caregivers and children aged two to five years were selected. The children were clinically evaluated and classified according to caries experience and severity of active caries. The Brazilian Portuguese version of the Early Childhood Oral Health Impact Scale (ECOHIS) was used to assess OHRQoL. Genotyping analysis of genetic polymorphisms in TNF- α (rs1799724, rs1799964, and rs1800629) was performed using real-time polymerase chain reaction. In the recessive model, children with the CC genotype of TNF-α (rs1799964) had a significantly high chance of poor OHRQoL in the symptom domain (pain), in both the caries experience (p = 0.045) and the high-severity active caries phenotypes (p = 0.033) (Mann-Whitney U test). It was concluded that genetic polymorphisms in TNF-α are associated with OHRQoL related to the symptom domain (pain), suggesting that TNF-α could be used as a potential biomarker for OHRQoL. Understanding the genetic aspects associated with OHRQoL will allow the early identification of patients with OHRQoL disparities and provide personalized healthcare.

Abstract This study aimed to evaluate the associations between oral health-related quality of life (OHRQoL) and patient-associated factors and polymorphisms in the estrogen receptor 1 (ESR1) and 2 (ESR2) genes in patients with dentofacial deformities (DFD). This cross-sectional study included 234 adult individuals. Data such as age, sex, and the type of facial profile (I, II, or III), were collected, and the short-form oral health impact profile 14 (OHIP-14) questionnaire was used to assess their OHRQoL. DNA was collected from oral mucosa cells, and the polymorphisms in ESR1 (rs2234693 and rs9340799) and ESR2 (rs1256049 and rs4986938) were evaluated using real-time polymerase chain reaction. The data were subjected to statistical analysis at a significance level of 5%. Individuals over 28 years of age exhibited worse OHRQoL (p = 0.003) than individuals aged less than or equal to 28 years. Women had worse OHRQoL than men (p < 0.001). Profile II individuals had worse OHRQoL in the social disability domain than profile III individuals (p = 0.030). Genetic analysis showed that rs9340799 was associated with OHRQoL in the functional limitation domain, and GG individuals exhibited worse OHRQoL than individuals carrying the AA/AG genotypes (p < 0.030). In the social handicap domain, individuals with GG genotype in rs9340799 exhibited worse OHRQoL than AG individuals (p < 0.043). Collectively, our results reveal that factors including age, sex, and type of facial profile, are associated with OHRQoL in patients with DFD. In addition, individuals with the GG genotype in rs9340799 (ESR1) may experience a negative impact on OHRQoL in the functional limitation and social handicap domains.

RESUMO O objetivo do presente estudo foi investigar a associação entre polimorfismos de nucleotídeo único (SNPs) em genes que codificam receptores de estrógeno (ESR1 e ESR2, respectivamente) e o retardo na emergência dentária (DTE). Este estudo transversal foi composto por crianças biológicas não relacionadas de ambos os sexos, com idades entre 11 e 13 anos. O DTE foi definido pela presença do dente decíduo na cavidade bucal após seu tempo e também, quando as crianças apresentaram pelo menos um dente permanente com atraso. O DNA genômico foi usado para avaliar os SNPs em ESR1 (rs9340799 e rs2234693) e ESR2 (rs1256049 e rs4986938) usando PCR em tempo real. Foram realizados testes Qui-quadrado ou exato de Fisher e Regressão Logística ajustados por idade e sexo. A interação SNP-SNP foi acessada pela análise de redução de dimensionalidade multifatorial (MDR), também ajustada por sexo e idade. O alfa de 5% foi estabelecido. Entre 537 crianças incluídas, 296 (55%) estavam no grupo “DTE” e 241 (45%) estavam no grupo “Controle”. A idade e o sexo não foram estatisticamente diferentes entre os grupos (p> 0,05). A distribuição de genótipos dos SNPs rs9340799, rs2234693, rs1256049 e rs4986938 não foi associada ao DTE (p> 0,05). Os modelos eleitos pelo MDR também não foram estatisticamente significativos. Conclusões: Os SNPs estudados na ESR1 e ESR2 não foram associados ao DTE na dentição permanente.

Abstract The purpose of the study was to investigate the association between single nucleotide polymorphisms (SNPs) in genes encoding estrogen receptors (ESR1 and ESR2, respectively) and delayed tooth emergence (DTE). This cross-sectional study was composed of biological unrelated children of both sexes, age ranging from 11 to 13 years old. DTE was defined when the successor primary tooth was still present in the oral cavity after its exfoliation time or the absence of the permanent tooth emergence into the oral cavity. Children were diagnosed with DTE when they had at least one delayed permanent tooth, according to age of exfoliation of each tooth proposed by The American Dental Association. Genomic DNA from saliva was used to evaluate the SNPs in ESR1 (rs9340799 and rs2234693) and ESR2 (rs1256049 and rs4986938) using Real-Time PCR. Chi-square or Fisher exact tests and Logistic Regression adjusted by age and gender were performed. SNP-SNP interaction was accessed by multifactor dimensionality reduction (MDR) analysis also adjusted by gender and age. The established alpha of this study was 5%. Among 537 included children, 296 (55%) were in the “DTE” group and the 241 (45%) were in the “Control” group. Age and gender were not statistically different among the groups (p>0.05). Genotype distribution of the SNPs rs9340799, rs2234693, rs1256049 and rs4986938 were not associated with DTE (p> 0.05). The models elected by MDR were not statistically significant either. Conclusions: The studied SNPs in ESR1 and ESR2 were not associated with permanent DTE.