The essential oil extracted from Anemia tomentosa (EOAT) has shown larvicidal potential against Aedes aegypti, based on methods in vitro and in silico. Chromatographic and spectrometric techniques (gas chromatography-mass spectrometry (CG-MS), gas chromatography-flame ionization detector (GC-FID)), as well as mono and bidimensional nuclear magnetic resonance spectroscopy (NMR) were used to assess 10 essential oil components. Chemical composition of essential oil comprised 87.51% sesquiterpenes, with emphasis on presilphiperfolan-1-ol (42.13%) and silphiperfol-5-ene (19.47%). Larvicidal assay conducted in vitro with EOAT has evidenced potential cytotoxic activity up to 48 h exposure to it; mortality rate observed for A. aegypti larvae exposed to essential oil reached 100%. Study conducted in silico with chemical compounds deriving from the herein investigated plant species has evidenced its potential to inhibit acetylcholinesterase in A. aegypti. Activity of triquinane sesquiterpenes ranging from -6.8 to -8.2 kcal mol-1 stood out in comparison to that of temephos (-7.5 kcal mol-1). Chemical compounds identified in the investigated essential oil presented low human and environmental toxicity, as observed in absorption, distribution, metabolism and excretion, and toxicity (ADMETox) predictions. (EOAT chromatographymass chromatography mass CGMS, CGMS CG MS , (CG-MS) chromatographyflame flame GCFID, GCFID GC FID (GC-FID)) NMR (NMR 1 components 8751 87 51 87.51 presilphiperfolan1ol presilphiperfolanol presilphiperfolan ol 42.13% 4213 42 13 (42.13% silphiperfol5ene silphiperfolene silphiperfol 5 ene 19.47%. 1947 19.47% . 19 47 (19.47%) 4 it A 100 100% 6.8 68 6 8 -6. 8.2 82 2 -8. mol1 mol mol- 7.5 75 7 (-7. mol1. mol-1) absorption distribution excretion ADMETox (ADMETox predictions (CG-MS (GC-FID) 875 87.5 42.13 421 (42.13 194 19.47 (19.47% 6. -6 8. -8 7. (-7 (GC-FID 87. 42.1 (42.1 19.4 (19.47 - (- 42. (42. 19. (19.4 ( (42 (19. (4 (19 (1

Bredemeyera Willd. is a genus of the Polygalaceae family, occurring in Brazil. This research aims to evaluate the phytochemical and antifungal potential, in vitro and in silico, of Bredemeyera brevifolia extracts. The identification of fatty acids by gas chromatography-mass spectrometry and their quantification by gas chromatography-flame ionization detector showed that palmitic (36.55 mg g−1), margaric (3.66 mg g−1), linolelaidic (108.82 mg g−1), oleic (7.86 mg g−1), stearic (41.11 mg g−1), dihomo-γ-linolenic (85.48 mg g−1), and arachidic (209.00 mg g−1) acids are present in the hexane extract. From the chloroform extract, 1-hydroxy-3,7-dimethoxyxanthone was isolated and identified. The minimum inhibitory concentration assay with methanolic extract demonstrated antifungal activity against species of the genus Candida, Sporothrix, and Trichophyton for up to 96 h, with values ranging from 64-1024 μg mL−1. The in silico study with the chemical compounds of this species showed fungal inhibition potential of sterol-14-α-demethylase, highlighting 1-hydroxy-3,7-dimethoxyxanthone, which presented an affinity energy of -8.8 kcal mol−1, greater than that observed for fluconazole (−8.3 kcal mol−1). The fatty acids interacted with the active site of the enzyme but presented energies ≥ −7.4 kcal mol−1. Compounds isolated from this species have pharmacokinetic potential for the development of pharmaceutical formulations. Willd family Brazil extracts chromatographymass chromatography mass chromatographyflame flame 36.55 3655 36 55 (36.5 g−1, g1 g g−1 , 1 3.66 366 3 66 (3.6 108.82 10882 108 82 (108.8 7.86 786 7 86 (7.8 41.11 4111 41 11 (41.1 dihomoγlinolenic dihomo γ linolenic 85.48 8548 85 48 (85.4 209.00 20900 209 00 (209.0 1hydroxy3,7dimethoxyxanthone 1hydroxy37dimethoxyxanthone hydroxydimethoxyxanthone hydroxy 3,7 dimethoxyxanthone identified Candida Sporothrix 9 h 641024 64 1024 64-102 mL1 mL mL−1 sterol14αdemethylase, sterol14αdemethylase sterolαdemethylase sterol 14 α demethylase, demethylase sterol-14-α-demethylase 1hydroxy3,7dimethoxyxanthone, dimethoxyxanthone, 8.8 88 8 -8. mol1 mol mol−1 −8.3 83 (−8. . mol−1) 74 4 −7. formulations 36.5 365 5 (36. g− 3.6 6 (3. 108.8 1088 10 (108. 7.8 78 (7. 41.1 411 (41. 85.4 854 (85. 209.0 2090 20 0 (209. 1hydroxy3 7dimethoxyxanthone 37 3, 64102 102 64-10 mL− αdemethylase 8. -8 mol− −8. (−8 −7 36. (36 3. (3 108. (108 7. (7 41. (41 85. (85 209. 2 (209 1hydroxy 6410 64-1 - −8 (− − ( (10 (4 (8 (20 641 64- (1 (2

Abstract The disease coronavirus COVID-19 has been the cause of millions of deaths worldwide. Among the proteins of SARS-CoV-2, non-structural protein 12 (NSP12) plays a key role during COVID infection and is part of the RNA-dependent RNA polymerase complex. The monitoring of NSP12 polymorphisms is extremely important for the design of new antiviral drugs and monitoring of viral evolution. This study analyzed the NSP12 mutations detected in circulating SARS-CoV-2 during the years 2020 to 2022 in the population of the city of Manaus, Amazonas, Brazil. The most frequent mutations found were P323L and G671S. Reports in the literature indicate that these mutations are related to transmissibility efficiency, which may have contributed to the extremely high numbers of cases in this location. In addition, two mutations described here (E796D and R914K) are close and have RMSD that is similar to the mutations M794V and N911K, which have been described in the literature as influential on the performance of the NSP12 enzyme. These data demonstrate the need to monitor the emergence of new mutations in NSP12 in order to better understand their consequences for the treatments currently used and in the design of new drugs. COVID19 19 COVID-1 worldwide SARSCoV2, SARSCoV2 SARSCoV SARS CoV 2, 2 nonstructural non structural 1 NSP (NSP12 RNAdependent dependent complex NSP1 evolution SARS-CoV- 202 Manaus Amazonas Brazil PL P L G671S GS G S efficiency location addition E796D ED E D R914K RK R K MV M V N911K NK N enzyme COVID1 COVID- (NSP1 SARS-CoV 20 (NSP

La distorsión de la imagen corporal es una alteración en la percepción del cuerpo que puede repercutir en la salud. Este estudio busca estimar, entre las mujeres participantes del Estudio Longitudinal de Salud del Adulto (ELSA-Brasil) que viven en Bahía, Brasil, la prevalencia de precisión y distorsión de la imagen corporal e investigar asociaciones con las características socioeconómicas, el estilo de vida y la busca de atención ginecológica. Participaron 609 mujeres que tenían entre 50 y 69 años que contestaron los cuestionarios aplicados cara a cara entre 2012 y 2014. Se utilizó la escala de siluetas de Stunkard para investigar la percepción precisa o distorsionada para más o menos peso. El cociente de riesgo relativo (RR) se calculó a través de regresión logística multinomial utilizando el Stata 13. La mayoría de los participantes tiene una perspectiva precisa del propio cuerpo (53,7%). Entre las personas con percepción distorsionada hay una tendencia a la distorsión para menos peso (38,1%). En el análisis de regresión multinomial, las variables raza/color y escolaridad permanecieron asociadas con la distorsión para menos peso, siendo la primera positivamente asociada con la distorsión para menos peso entre las mujeres pardas (RR = 1,89; IC95%: 1,13-3,16) y negras (RR = 2,10; IC95%: 1,25-3,55), mientras la segunda entre las mujeres que estudiaron hasta la enseñanza secundaria (RR = 1,65; IC95%: 1,18-2,33). No hubo asociaciones con las otras variables ni con la distorsión para más peso. Los resultados contribuyen para explicar las relaciones entre la percepción de la imagen corporal y los factores socioeconómicos, demostrando que mujeres de diferentes razas/colores y diferentes niveles de educación se influyen de distintas formas a través de discursos sociales, lo que impacta en la percepción de su imagen corporal. salud estimar ELSABrasil ELSA Brasil (ELSA-Brasil Bahía socioeconómicas ginecológica 60 5 6 201 2014 RR 13 53,7%. 537 53,7% . 53 7 (53,7%) 38,1%. 381 38,1% 38 1 (38,1%) razacolor raza color 1,89 189 89 IC95% IC95 IC 1,133,16 113316 1,13 3,16 3 16 1,13-3,16 2,10 210 2 10 1,253,55, 125355 1,25 3,55 , 25 55 1,25-3,55) 1,65 165 65 1,182,33. 118233 1,18 2,33 18 33 1,18-2,33) socioeconómicos razascolores razas colores sociales 20 53,7 (53,7% 38,1 (38,1% 1,8 8 IC9 133 1,133,1 11331 113 1,1 316 3,1 1,13-3,1 2,1 21 253 1,253,55 12535 125 1,2 355 3,5 1,25-3,55 1,6 182 1,182,33 11823 118 233 2,3 1,18-2,33 53, (53,7 38, (38,1 1, 1,133, 1133 11 31 3, 1,13-3, 2, 1,253,5 1253 12 35 1,25-3,5 1,182,3 1182 23 1,18-2,3 (53, (38, 1,133 1,13-3 1,253, 1,25-3, 1,182, 1,18-2, (53 (38 1,13- 1,253 1,25-3 1,182 1,18-2 (5 (3 1,25- 1,18- (

Body image distortion is an alteration in the perception of the body that can have repercussions on health. This study aims to estimate the prevalence of body image accuracy and distortion among women participating in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) living in Bahia State, Brazil, and to investigate associations with socioeconomic characteristics, lifestyle, and gynecological care seeking. A total of 609 women aged 50 to 69 years participated in the study, who answered face-to-face questionnaires from 2012 to 2014. The Stunkard silhouette scale was used to investigate accurate or distorted perception for more or less weight. The relative risk ratio (RR) was calculated by multinomial logistic regression using Stata 13. Most participants have an accurate perception of their own bodies (53.7%). Among those with distorted perception, there is a tendency to distort towards less weight (38.1%). In the multinomial regression analysis, the variables race/skin color and education remained associated with the distortion towards underweight. The race/skin color variable was positively associated with the distortion towards underweight among Mixed-race women (RR = 1.89; 95%CI: 1.13-3.16) and black (RR = 2.10; 95%CI: 1.25-3.55), while the education variable among those with up to high school education (RR = 1.65; 95%CI: 1.18-2.33). There were no associations with the other variables or with distortion for more weight. The results contribute to explaining the relationships between body image perception and socioeconomic factors, revealing that women of different races/skin colors and varying educational levels are influenced in different ways by social discourses, impacting the perception of their body image. health ELSABrasil ELSA Brasil (ELSA-Brasil State Brazil characteristics lifestyle seeking 60 5 6 facetoface face 201 2014 RR 13 53.7%. 537 53.7% . 53 7 (53.7%) 38.1%. 381 38.1% 38 1 (38.1%) analysis raceskin race skin Mixedrace Mixed 1.89 189 89 95%CI 95CI CI 95 1.133.16 113316 1.13 3.16 3 16 1.13-3.16 2.10 210 2 10 1.253.55, 125355 1.25 3.55 , 25 55 1.25-3.55) 1.65 165 65 1.182.33. 118233 1.18 2.33 18 33 1.18-2.33) factors racesskin races discourses 20 53.7 (53.7% 38.1 (38.1% 1.8 8 9 133 1.133.1 11331 113 1.1 316 3.1 1.13-3.1 2.1 21 253 1.253.55 12535 125 1.2 355 3.5 1.25-3.55 1.6 182 1.182.33 11823 118 233 2.3 1.18-2.33 53. (53.7 38. (38.1 1. 1.133. 1133 11 31 3. 1.13-3. 2. 1.253.5 1253 12 35 1.25-3.5 1.182.3 1182 23 1.18-2.3 (53. (38. 1.133 1.13-3 1.253. 1.25-3. 1.182. 1.18-2. (53 (38 1.13- 1.253 1.25-3 1.182 1.18-2 (5 (3 1.25- 1.18- (

Distorção da imagem corporal é uma alteração da percepção do corpo que pode repercutir na saúde. Este estudo visa estimar, entre mulheres participantes do Estudo Longitudinal de Saúde do Adulto (ELSA-Brasil) residentes na Bahia, Brasil, a prevalência de acurácia e distorção da imagem corporal e investigar associações com características socioeconômicas, estilo de vida e procura de cuidados ginecológicos. Participaram 609 mulheres de 50-69 anos de idade que responderam, entre 2012-2014, questionários aplicados face a face. Foi utilizada a escala de silhuetas de Stunkard para investigar a percepção acurada ou distorcida para mais ou menos peso. A razão de risco relativo (RR) foi calculada por meio de regressão logística multinomial por meio do Stata 13. A maioria das participantes tem perspectiva acurada do próprio corpo (53,7%). Entre aquelas com percepção distorcida, há uma tendência à distorção para menos peso (38,1%). Na análise de regressão multinomial, permaneceram associadas à distorção para menos peso as variáveis raça/cor e escolaridade, sendo que a primeira foi positivamente associada à distorção para menos peso entre as pardas (RR = 1,89; IC95%: 1,13-3,16) e pretas (RR = 2,10; IC95%: 1,25-3,55), enquanto a segunda entre aquelas com escolaridade até o Ensino Médio (RR = 1,65; IC95%: 1,18-2,33). Não houve associações quanto às demais variáveis, nem com distorção para mais peso. Os resultados contribuem para a explicação das relações entre percepção da imagem corporal e fatores socioeconômicos, revelando que mulheres de raça/cor diferentes e variados níveis de escolaridade são influenciadas de formas distintas pelos discursos sociais, o que impacta a percepção da sua imagem corporal. saúde estimar ELSABrasil ELSA Brasil (ELSA-Brasil Bahia socioeconômicas ginecológicos 60 5069 50 69 50-6 responderam 20122014, 20122014 2012 2014, 2014 2012-2014 RR 13 53,7%. 537 53,7% . 53 7 (53,7%) 38,1%. 381 38,1% 38 1 (38,1%) raçacor raça cor 1,89 189 89 IC95% IC95 IC 1,133,16 113316 1,13 3,16 3 16 1,13-3,16 2,10 210 2 10 1,253,55, 125355 1,25 3,55 , 25 55 1,25-3,55) 1,65 165 65 1,182,33. 118233 1,18 2,33 18 33 1,18-2,33) socioeconômicos sociais 6 506 5 50- 2012201 201 2012-201 53,7 (53,7% 38,1 (38,1% 1,8 8 IC9 133 1,133,1 11331 113 1,1 316 3,1 1,13-3,1 2,1 21 253 1,253,55 12535 125 1,2 355 3,5 1,25-3,55 1,6 182 1,182,33 11823 118 233 2,3 1,18-2,33 201220 20 2012-20 53, (53,7 38, (38,1 1, 1,133, 1133 11 31 3, 1,13-3, 2, 1,253,5 1253 12 35 1,25-3,5 1,182,3 1182 23 1,18-2,3 20122 2012-2 (53, (38, 1,133 1,13-3 1,253, 1,25-3, 1,182, 1,18-2, 2012- (53 (38 1,13- 1,253 1,25-3 1,182 1,18-2 (5 (3 1,25- 1,18- (

Abstract Here we studied the entire Atlantic Forest hotspot to investigate whether the effect of different environmental predictors depends on the phylogenetic extension and the biogeographical history of different Atlantic Forest sectors. We used occurrence data of 3,183 plant species with arboreal or arborescent habits. We reconstructed climatic stability across 120,000 years using the Random Forest method. Then, we compared the effect of biogeographical history, topographic, and climatic variables on species richness and phylogenetic diversity using Geographically Weighted Regression (GWR) models. Niche conservatism drives the strength and direction of environmental correlates with tree diversity, interacting with the biogeographical and phylogenetic extension considered. Low current climate seasonalities were the main drivers of species richness and phylogenetic diversity variation across the Atlantic Forest. Whereas in higher phylogenetic extension, topographic heterogeneity increased the number of tree species independent of the sector, deep-past climate stability favored phylogenetic diversity by increasing relict lineages of distant clades in all forests, but with anomalies in the southern sector. This investigation yields substantial evidence that the response of the northern and southern sectors of the Atlantic Forest to identical environmental conditions diverges significantly, providing compelling support for the imprint of phylogenetic heritage in generating non-linear diversity patterns. 3183 3 183 3,18 habits 120000 120 000 120,00 method Then GWR (GWR models considered sector deeppast deep past forests significantly nonlinear non linear patterns 318 18 3,1 12000 12 00 120,0 31 1 3, 1200 0 120,

The potential of four commercial clones of unripe acerola fruit was investigated regarding a source of nutraceuticals and functional foods for industrial use. The chemical profiling of the BRS 235-Apodi, BRS 236-Cereja, BRS 237-Roxinha, and BRS 238-Frutacor clones was determined by the ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS-MS) technique coupled to multivariate statistical analysis, which were then correlated to ABTS•+ radical capture (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and ferric reducing antioxidant power (FRAP) assays in order to measure the antioxidant capacity. A total of 24 bioactive compounds were identified, including high amounts of quercetin-O-hexoside, astilbin, and apigenin, which were correlated to three clones (BRS 235, BRS 237, and BRS 238). On the other hand, the BRS 236 clone presented high contents of ascorbic acid, iso-ascorbic acid, citric acid, procyanidin B trimer, rutin, phenolic compounds, and antioxidant activity. These compounds were additionally found to be strongly associated with the antioxidant capacity of unripe acerola fruits. Therefore, the present study revealed that BRS 236 is a promising clone as an antioxidant source to be used as a functional food or ingredient. use 235Apodi, 235Apodi Apodi 235 Apodi, 235-Apodi 236Cereja, 236Cereja Cereja Cereja, 236-Cereja 237Roxinha, 237Roxinha Roxinha 237 Roxinha, 237-Roxinha 238Frutacor Frutacor 238 ultrahigh ultra chromatographyquadrupole chromatography quadrupole timeofflight time flight UPLCQTOF/MSMS UPLCQTOFMSMS UPLC QTOF/MS MS QTOF (UPLC-QTOF/MS-MS analysis ABTS ABTS• 2,2azinobis3ethylbenzothiazoline6sulfonic 22azinobis3ethylbenzothiazoline6sulfonic azinobisethylbenzothiazolinesulfonic 2,2 azino bis 3 ethylbenzothiazoline 6 sulfonic 2 acid FRAP (FRAP identified quercetinOhexoside, quercetinOhexoside quercetin O hexoside, hexoside quercetin-O-hexoside astilbin apigenin 238. . 238) hand 23 isoascorbic iso trimer rutin activity fruits Therefore ingredient UPLCQTOF MSMS QTOFMS 2azinobis3ethylbenzothiazoline6sulfonic azinobis 22 2,

Determining chemical profiles of complex matrices, such as beers of different styles, can highlight regional characteristics and provide robust literature on the variability of this product, improving quality control. A practical application is to unequivocally attribute the authenticity of the product even in the face of variations in composition due to inadequate transport and storage. This work used proton nuclear magnetic resonance (1H NMR) spectroscopy and chemometrics in the semi-quantitative study of Brazilian ale and lager beers. The results demonstrated the success of applying 1H NMR in characterizing chemical profiles and as a statistical database to distinguish ale and lager beers. Using principal component analysis (PCA) of the NMR data, it was possible to identify that carbohydrate content was responsible for the separation tendency between these beer styles. Ale beers had a higher residual carbohydrate content, according to the integrals of the carbohydrate hydrogens. This is expected, as these beers are obtained by fermentation at higher temperatures for shorter fermentation times. The paper also described soft independent modelling of class analogies (SIMCA) as applied to the NMR data. This class model made it possible to correctly classify 90% of the samples as ale and 100% as lager. matrices styles control storage H semiquantitative semi quantitative PCA (PCA data hydrogens expected times SIMCA (SIMCA 90 100 9 10 1

Abstract Cowpea is a leguminous plant belonging to the fabaceae family cultivated in the North and Northeast regions of Brazil, with productive potential. Among the abiotic factors, water deficiency is one of the main environmental limitations that influence agricultural production in the world. The objective of this work was to study the relative water content and osmoregulators of cowpea plants subjected to water stress. The experiment was carried out in a greenhouse at the Universidade Federal Rural da Amazônia (UFRA, Belém, PA), cowpea plants BR-17 Gurguéia Vigna unguiculata (L.) Walp were used. The experimental design was completely randomized (DIC) in a 2 × 2 factorial scheme, two water conditions (control and water deficit) and two times of stress (four and six days of water suspension), with 7 replications, totaling 28 experimental units. The water deficit affected plants, causing a reduction in relative water content (69.98%), starch (12.84% in leaves and 23.48% in roots) and carbohydrates (84.34%), and an increase in glycine-betaine, sucrose (114.11% in leaves and 18.71% in roots) and proline (358.86%) at time 2. The relative water content was negatively affected by water conditions, with a decrease in relation to the interaction of the aerial part and the root system. Therefore, greater metabolic responses were noted in plants that were subjected to stress treatment at time 2 (6 days). Brazil potential factors world UFRA, UFRA (UFRA Belém PA, PA , PA) BR17 BR 17 BR-1 L. L (L. used DIC (DIC scheme control four suspension, suspension suspension) replications units 69.98%, 6998 69.98% 69 98 (69.98%) 12.84% 1284 12 84 (12.84 2348 23 48 23.48 roots 84.34%, 8434 84.34% 34 (84.34%) glycinebetaine, glycinebetaine glycine betaine, betaine glycine-betaine 114.11% 11411 114 11 (114.11 1871 18 71 18.71 358.86% 35886 358 86 (358.86% system Therefore 6 ( days. . days) BR1 1 BR- (L 699 69.98 9 (69.98% 12.84 128 8 (12.8 234 4 23.4 843 84.34 3 (84.34% 114.11 1141 (114.1 187 18.7 358.86 3588 35 (358.86 69.9 (69.98 12.8 (12. 23. 84.3 (84.34 114.1 (114. 18. 358.8 (358.8 69. (69.9 12. (12 84. (84.3 114. (114 358. (358. (69. (1 (84. (11 (358 (69 (84 (35 (8 (3

Resumo O feijão-caupi é uma planta leguminosa pertencente à família das Fabaceae cultivada nas regiões Norte e Nordeste do Brasil, com potencial produtivo. Entre os fatores abióticos, a deficiência hídrica é uma das principais limitações ambientais que influenciam a produção agrícola no mundo. O objetivo do trabalho foi estudar o conteúdo relativo de água e os osmorreguladores de plantas de feijão-caupi submetidas ao estresse hídrico. O experimento foi conduzido em casa de vegetação da Universidade Federal Rural da Amazônia (UFRA, Belém, PA), foram utilizadas plantas de feijão-caupi BR-17 Gurguéia Vigna unguiculata (L.) Walp. O delineamento experimental foi inteiramente casualizado (DIC) em esquema fatorial 2 × 2, duas condições hídricas (controle e déficit hídrico) e dois tempos de estresse (quatro e seis dias de suspensão hídrica), com 7 repetições, totalizando 28 unidades experimentais. O estresse hídrico afetou as plantas, provocando redução no conteúdo relativos de água (69,98%), amido (12,84% nas folhas e 23,48% nas raízes) e carboidratos (84,34%), e aumento na glicina-betaína, sacarose (114,11% nas folhas e 18,71% nas raízes) e prolina (358,86%) no tempo 2. O conteúdo relativo de água foi afetado negativamente pelas condições hídricas, com diminuição em relação à interação da parte aérea e do sistema radicular. Diante disso, notou-se maiores respostas metabólicas nas plantas que foram submetidas ao tratamento de estresse no tempo 2 (6 dias). feijãocaupi feijão caupi Brasil produtivo abióticos mundo UFRA, UFRA (UFRA Belém PA, PA , PA) BR17 BR 17 BR-1 L. L (L. Walp DIC (DIC controle quatro hídrica, hídrica) repetições experimentais 69,98%, 6998 69,98% 69 98 (69,98%) 12,84% 1284 12 84 (12,84 2348 23 48 23,48 raízes 84,34%, 8434 84,34% 34 (84,34%) glicinabetaína, glicinabetaína glicina betaína, betaína glicina-betaína 114,11% 11411 114 11 (114,11 1871 18 71 18,71 358,86% 35886 358 86 (358,86% radicular disso notouse notou se 6 ( dias. . dias) BR1 1 BR- (L 699 69,98 9 (69,98% 12,84 128 8 (12,8 234 4 23,4 843 84,34 3 (84,34% 114,11 1141 (114,1 187 18,7 358,86 3588 35 (358,86 69,9 (69,98 12,8 (12, 23, 84,3 (84,34 114,1 (114, 18, 358,8 (358,8 69, (69,9 12, (12 84, (84,3 114, (114 358, (358, (69, (1 (84, (11 (358 (69 (84 (35 (8 (3

La distorsión de la imagen corporal es una alteración en la percepción del cuerpo que puede repercutir en la salud. Este estudio busca estimar, entre las mujeres participantes del Estudio Longitudinal de Salud del Adulto (ELSA-Brasil) que viven en Bahía, Brasil, la prevalencia de precisión y distorsión de la imagen corporal e investigar asociaciones con las características socioeconómicas, el estilo de vida y la busca de atención ginecológica. Participaron 609 mujeres que tenían entre 50 y 69 años que contestaron los cuestionarios aplicados cara a cara entre 2012 y 2014. Se utilizó la escala de siluetas de Stunkard para investigar la percepción precisa o distorsionada para más o menos peso. El cociente de riesgo relativo (RR) se calculó a través de regresión logística multinomial utilizando el Stata 13. La mayoría de los participantes tiene una perspectiva precisa del propio cuerpo (53,7%). Entre las personas con percepción distorsionada hay una tendencia a la distorsión para menos peso (38,1%). En el análisis de regresión multinomial, las variables raza/color y escolaridad permanecieron asociadas con la distorsión para menos peso, siendo la primera positivamente asociada con la distorsión para menos peso entre las mujeres pardas (RR = 1,89; IC95%: 1,13-3,16) y negras (RR = 2,10; IC95%: 1,25-3,55), mientras la segunda entre las mujeres que estudiaron hasta la enseñanza secundaria (RR = 1,65; IC95%: 1,18-2,33). No hubo asociaciones con las otras variables ni con la distorsión para más peso. Los resultados contribuyen para explicar las relaciones entre la percepción de la imagen corporal y los factores socioeconómicos, demostrando que mujeres de diferentes razas/colores y diferentes niveles de educación se influyen de distintas formas a través de discursos sociales, lo que impacta en la percepción de su imagen corporal.

Distorção da imagem corporal é uma alteração da percepção do corpo que pode repercutir na saúde. Este estudo visa estimar, entre mulheres participantes do Estudo Longitudinal de Saúde do Adulto (ELSA-Brasil) residentes na Bahia, Brasil, a prevalência de acurácia e distorção da imagem corporal e investigar associações com características socioeconômicas, estilo de vida e procura de cuidados ginecológicos. Participaram 609 mulheres de 50-69 anos de idade que responderam, entre 2012-2014, questionários aplicados face a face. Foi utilizada a escala de silhuetas de Stunkard para investigar a percepção acurada ou distorcida para mais ou menos peso. A razão de risco relativo (RR) foi calculada por meio de regressão logística multinomial por meio do Stata 13. A maioria das participantes tem perspectiva acurada do próprio corpo (53,7%). Entre aquelas com percepção distorcida, há uma tendência à distorção para menos peso (38,1%). Na análise de regressão multinomial, permaneceram associadas à distorção para menos peso as variáveis raça/cor e escolaridade, sendo que a primeira foi positivamente associada à distorção para menos peso entre as pardas (RR = 1,89; IC95%: 1,13-3,16) e pretas (RR = 2,10; IC95%: 1,25-3,55), enquanto a segunda entre aquelas com escolaridade até o Ensino Médio (RR = 1,65; IC95%: 1,18-2,33). Não houve associações quanto às demais variáveis, nem com distorção para mais peso. Os resultados contribuem para a explicação das relações entre percepção da imagem corporal e fatores socioeconômicos, revelando que mulheres de raça/cor diferentes e variados níveis de escolaridade são influenciadas de formas distintas pelos discursos sociais, o que impacta a percepção da sua imagem corporal.

Body image distortion is an alteration in the perception of the body that can have repercussions on health. This study aims to estimate the prevalence of body image accuracy and distortion among women participating in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) living in Bahia State, Brazil, and to investigate associations with socioeconomic characteristics, lifestyle, and gynecological care seeking. A total of 609 women aged 50 to 69 years participated in the study, who answered face-to-face questionnaires from 2012 to 2014. The Stunkard silhouette scale was used to investigate accurate or distorted perception for more or less weight. The relative risk ratio (RR) was calculated by multinomial logistic regression using Stata 13. Most participants have an accurate perception of their own bodies (53.7%). Among those with distorted perception, there is a tendency to distort towards less weight (38.1%). In the multinomial regression analysis, the variables race/skin color and education remained associated with the distortion towards underweight. The race/skin color variable was positively associated with the distortion towards underweight among Mixed-race women (RR = 1.89; 95%CI: 1.13-3.16) and black (RR = 2.10; 95%CI: 1.25-3.55), while the education variable among those with up to high school education (RR = 1.65; 95%CI: 1.18-2.33). There were no associations with the other variables or with distortion for more weight. The results contribute to explaining the relationships between body image perception and socioeconomic factors, revealing that women of different races/skin colors and varying educational levels are influenced in different ways by social discourses, impacting the perception of their body image.

RESUMO A diversificação de cultivares e o cultivo consorciado com capim-limão podem ser uma abordagem inovadora para os produtores de banana aumentarem a variedade, a rentabilidade e a sustentabilidade dos pomares e se adaptarem às mudanças climáticas. O estudo foi conduzido no estado de São Paulo, uma região subtropical do sudeste do Brasil, e avaliou a duração dos ciclos de cultivo, o desempenho do crescimento e da produção e os teores nutricionais foliares das cultivares de bananeira BRS FHIA Maravilha, SCS 451 Prata Catarina, BRS Pacoua e Prata Anã em consórcio com capim-limão. O experimento de campo foi conduzido durante dois ciclos de cultivo consecutivos. O crescimento, a produtividade e o teor de nutrientes nas folhas das cultivares de bananeira apresentaram o mesmo desempenho em monocultura e em consórcio com capim-limão. O ciclo mais curto foi observado apenas na primeira safra com a cultura intercalar de capim-limão. A cultivar Prata Anã se destacou pelo ciclo mais precoce, menor altura de plantas e maior produtividade média por ano. Esses resultados são úteis no planejamento da diversificação de cultivares para novos pomares, na identificação da possibilidade de consorciação com capim-limão e na determinação de genótipos e sistemas de cultivo mais adaptados às condições climáticas adversas. capimlimão capim limão variedade Paulo Brasil Maravilha 45 Catarina capimlimão. limão. consecutivos precoce ano adversas 4

ABSTRACT Diversifying cultivars and intercropping with lemongrass can innovative strategies for banana growers to enhance the variety, profitability and sustainability of their orchards and to adapt to climate change. This study took place in the state of São Paulo, a subtropical region of southeastern Brazil and assessed the duration of the cropping cycles, growth and yield performance, and leaf nutritional content of banana cultivars BRS FHIA Maravilha, SCS 451 Prata Catarina, BRS Pacoua and Prata Anã when intercropped with lemongrass. The field experiment spanned two consecutive growing seasons. The growth, yield and nutrient content in the leaves of banana cultivars exhibited similar performance in both monoculture and intercropping with lemongrass. A shorter cycle was observed only in the first harvest with the lemongrass intercrop. The Prata Anã cultivar stood out for its earlier cycle, lower plant height and higher average yield per year. These findings are valuable for planning cultivar diversification in new orchards, exploring the potential for intercropping with lemongrass, and identifying the genotypes and cropping systems that are better suited to adverse climatic conditions. variety change Paulo cycles Maravilha 45 Catarina seasons intercrop year conditions 4

Abstract This manuscript deals with a hierarchical control problem for Oldroyd equation under the Stackelberg-Nash strategy. The Oldroyd equation model is defined by non-regular coefficients, that is, they are bounded measurable functions. We assume that we can act in the dynamic of the system by a hierarchy of controls, where one main control (the leader) and several additional secondary control (the followers) act in order to accomplish their given tasks: controllability for the leader and optimization for followers. We obtain the existence and uniqueness of Nash equilibrium and its characterization, the approximate controllability with respect to the leader control, and the optimality system for leader control. StackelbergNash Stackelberg strategy nonregular non regular coefficients functions controls followers tasks characterization

RESUMO O surgimento do HIV impactou os serviços de saúde abruptamente em 1981, inicialmente afetando grupos sociais restritos. Atualmente, com o desenvolvimento da terapia antirretroviral, nos deparamos com uma doença diferente da encarada na década de 80, agora com uma evolução crônica. Todavia, algumas barreiras permanecem as mesmas, como o impacto social, mental e físico do diagnóstico na vida da pessoa que vive com HIV. O objetivo do presente estudo é verificar carga viral, contagem de linfócitos CD4+, e qualidade de vida de pessoas que vivem com o vírus da imunodeficiência humana. A presente pesquisa trata-se de um estudo observacional transversal com amostra de 24 indivíduos de ambos os sexos, com sorologia positiva para HIV, em acompanhamento médico sob tratamento antirretroviral. Foi utilizado um instrumento próprio para a coleta de informações sociodemográficas. Os resultados de contagem de CD4+ e a Carga Viral do HIV foram coletados dos prontuários médicos dos pacientes. A Qualidade de vida (QV) foi avaliada através do WHOQOL-HIV-BREF. Foi utilizada a análise descritiva para a caracterização do perfil da amostra. Os participantes (46,8± 9,2 anos) possuem ensino médio completo (41,8%), com renda de um a três salários mínimos (37,5%). Apresentam de um a três anos de diagnóstico e contagem de linfócitos CD4 acima de 500 mm3 de sangue (75%). Em relação à QV, os participantes apresentaram média mais alta para o domínio físico (26,7± 5,3) e mais baixa para os domínios de meio ambiente (10,9± 2,7) e relações sociais (12,2± 2,8). O estudo conclui que as pessoas que vivem com HIV participantes do estudo são adultos jovens, com renda e nível de escolaridade baixas, apresentam baixa qualidade de vida em relação aos domínios meio ambiente e relações sociais.

ABSTRACT The emergence of HIV abruptly impacted health services in 1981, initially affecting restricted social groups. Currently, with the development of antiretroviral therapy, we are faced with a different disease from the one seen in the 80s, now with a chronic evolution. However, some barriers remain the same, such as the social, mental and physical impact of the diagnosis on the life of the person living with HIV. The objective of the present study was to verify the viral load, CD4+ lymphocyte count, and quality of life of people living with the human immunodeficiency virus. This was a cross-sectional observational research with a sample of 24 individuals of both sexes, with positive serology for HIV, in medical follow-up under antiretroviral treatment. A specific instrument was used to collect sociodemographic information. CD4+ count and HIV viral load results were collected from patients’ medical records. Quality of life (QoL) was assessed using the WHOQOL-HIV-BREF. Descriptive analysis was used to characterise the sample profile. Participants (46.8± 9.2 years) have completed high school (41.8%), with an income of one to three minimum wages (37.5%). They present a three-year diagnosis and CD4 lymphocyte count above 500 mm3 of blood (75%). Regarding QoL, the participants had a higher average for the physical domain (26.7± 5.3) and lower averages for the environment domains (10.9± 2.7) and social relationships (12.2± 2.8). The study concludes that the people living with HIV who participated in the study are young adults, with low income and level of education, with a low quality of life in relation to the environment and social relations domains.

Pectis brevipedunculata is native species and widely available in dry and semi-arid ecosystems showing high biotechnological potential. The objective of this study was to evaluate the circadian and seasonal chemical variation of the essential oil (EO) of P. brevipedunculata, as well as its acaricide effect on Rhipicephalus microplus larvae. Aerial parts were collected and submitted to the hydrodistillation process, and the chemical composition was determined by gas chromatography mass spectrometry (GC-MS). For the assays with R. microplus, the larval immersion test was performed. The main constituents were citral (75% of the EO), followed by α-pinene and limonene. In the seasonality analysis, the highest yields were in the months of April (2.08%) and August (2.05%), while in the study of circadian rhythm, the percentage was 2.0% at 6 p.m. in the rainy season, and 1.2%, dry season at 6 p.m. Concerning acaricidal activity (50% lethal concentration (LC50)), the April (1.17 mg mL-1), March (1.28 mg mL-1), June (1.37 mg mL-1), and October (1.27 mg mL-1) oils obtained were the most active and assays performed with circadian rhythm revealed in the rain season (April) at 6 p.m. and dry season (September) at 12 a.m. LC50 values of 1.75 and 1.75 mg mL-1, respectively. Additionally, this EO is selective to non-target organisms, i.e., ladybeetles and lacewing. semiarid semi arid potential (EO P larvae process GCMS. GCMS GC MS . (GC-MS) R 75% 75 (75 EO, , EO) αpinene α pinene limonene analysis 2.08% 208 2 08 (2.08% 2.05%, 205 2.05% 05 (2.05%) 20 0 2.0 pm p m p.m 1 1.2% 50% 50 (50 LC50, LC (LC50)) 1.17 117 17 (1.1 mL1, mL1 mL 1.28 128 28 (1.2 1.37 137 37 (1.3 1.27 127 27 mL-1 (April September (September am a a.m LC5 175 1.7 1, respectively Additionally nontarget non target organisms ie i e i.e. lacewing (GC-MS 7 (7 2.08 (2.08 2.05 (2.05% 2. 1.2 5 (5 (LC50) 1.1 11 (1. 1.3 13 3 mL- 1. i.e ( (2.0 (2.05 (LC50 (1 (2. (LC5 (2 (LC

Research to identify plant bioactive compounds led to the evolution of extraction methods. This study optimized ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) to extract the flavonoids of Croton grewioides Baill. species contributing to characterize the polar extract profile and understand the antioxidant potential of these plant constituents. The use of experimental design and statistical treatments enabled the determination of the ideal conditions for each technique. The extracts of five accessions of C. grewioides were obtained under the optimized extraction conditions and were analyzed using ultra high-resolution mass spectrometry (FT-Orbitrap MS) operating at negative ionization mode for flavonoids detection. The accurate experimental mass obtained to the main compounds was used to attribute the molecular formula. Chemical structures of the main compounds detected were proposed using structure data bases. Chemometric analysis were performed with two FT-Orbitrap MS spectra samples using the identified metabolites and, the antioxidant activity data, showing that for this species the MAE was most effective in extracting the antioxidant compounds. It was possible to propose the structures for forty compounds in the C. grewioides extracts, demonstrating the excellent performance of the FT-Orbitrap MS in providing information on the chemical profile of polar compounds in plant extracts. methods ultrasoundassisted ultrasound assisted UAE (UAE microwaveassisted microwave (MAE Baill constituents technique C highresolution high resolution FTOrbitrap FT Orbitrap detection formula bases

This study focuses on the impact of Alzheimer’s Disease on language, namely the impact in the context of the use of the null subject. We analysed the productions of a group of twelve Alzheimer’s patients and a control group with the same dimension, when reading three types of sentences: One clause with simple tense; one clause with complex tense; two-clause sentences. The sentences, translated from a previous study of Italian and English, were repeated over three days by each of the participants and audio recorded for later data processing. Alzheimer’s patients underperformed when compared to the control group, but some results showed non-significant differences. When the data obtained in the study in Portugal was compared to that from the study conducted in England, it was concluded that the Alzheimer patients tended to produce more null subjects and that these were more prevalent in longer and complex sentences. The Portuguese participants also presented a tendency for the production of less null subjects than the Italians.

Com este estudo pretendemos conhecer melhor o impacto da Doença de Alzheimer (DA) na linguagem, mais concretamente o impacto no contexto da utilização do sujeito nulo. Analisamos as produções de doze pessoas com DA e de um grupo de controlo com a mesma dimensão, em tarefas de leitura de três tipos de frases: Simples com verbo em tempo simples; simples com verbo conjugado num tempo composto; complexas formadas por duas orações. As frases, traduzidas de um estudo prévio em italiano e inglês, foram repetidas ao longo de três dias por cada um dos participantes e gravadas para posterior tratamento de dados. As pessoas com DA apresentaram um desempenho inferior em relação ao grupo de controlo, sendo, no entanto, algumas diferenças entre os dois grupos, não significativas. Os dados recolhidos em Portugal quando comparados com os do estudo efetuado em Inglaterra, permitiram concluir que as pessoas com DA têm uma maior tendência para a produção de sujeitos nulos, tendência que se verifica com maior predominância nas frases mais longas e mais complexas. Os participantes portugueses apresentaram também uma menor tendência para a produção de sujeitos nulos do que os italianos.