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Abstract Background: Cardiovascular diseases are the leading cause of mortality worldwide and Mexico is no exception. The epidemiological data obtained in 1990 showed that cardiovascular diseases represented 19.8% of all causes of death in our country. This figure increased significantly to 25.5% for 2015. Some national surveys suggest that more than 60% of the adult population has at least one risk factor for cardiovascular disease (obesity or overweight, hypertension, smoking, diabetes, dyslipidemias). On the other hand, data from the Pan American Health Organization have linked the process of atherosclerosis as the first cause of premature death, significantly reducing life expectancy, which has enormous social repercussions. Objective: This document constitutes the Clinical Practice Guide (CPG) prepared at the initiative of the Mexican Society of Cardiology in collaboration with the Mexican Society of Nutrition and Endocrinology, AC, National Association of Cardiologists of Mexico, AC, Mexican Association for the Prevention of Atherosclerosis and its Complications, AC, National Normative Committee of General Medicine, AC, National College of Geriatric Medicine, AC, College of Internal Medicine of Mexico, AC, Mexican Society of Angiology and Vascular and Endovenous Surgery, AC, Mexican Institute of Research Nephrological, AC and the Mexican Academy of Neurology, A.C.; with the methodological support of the Ibero-American Agency for the Development and Evaluation of Health Technologies, in order to establish recommendations based on the best available evidence and agreed upon by an interdisciplinary group of experts. The objective of this document is to provide evidence-based recommendations to help decision makers in the diagnosis and treatment of dyslipidemias in our country. Material and methods: This document complies with international quality standards, such as those described by the Institute of Medicine of the USA, the Institute of Clinical Excellence of Great Britain, the Scottish Intercollegiate Guideline Network and the Guidelines International Network. A multidisciplinary group of clinical experts and methodologists with experience in systematic reviews of the literature and the development of clinical practice guidelines was formed. A scope document was agreed upon, relevant clinical questions were established, the best available evidence critically evaluated in systematic literature reviews was exhaustively identified, and clinical recommendations were developed. The modified Delphi Panel methodology was used to achieve an adequate level of consensus in each of the recommendations contained in this CPG. Results: 23 clinical questions were agreed upon which gave rise to their respective clinical recommendations. Conclusions: We consider that this document contributes to better clinical decision-making and becomes a point of reference for clinicians and patients in the management of dyslipidemias and this contributes to reducing the morbidity and mortality derived from atherosclerotic cardiovascular events in our country.

https://doi.org/10.24875/acm.m22000081 1458 downloads

Three Mexican Families with β thalassemia intermedia with different molecular basis
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Torre, Lourdes del Carmen Rizo de la

; Díaz, Francisco Javier Perea

; Cortés, Bertha Ibarra

; López, Víctor Manuel Rentería

; López, Josefina Yoaly Sánchez

; Anzaldo, Francisco Javier Sánchez

; Torres, María Teresa Magaña

; Gonnet, Katia

; Badens, Catherine

; Bonello-Palot, Nathalie

Genetics and Molecular Biology

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2019, Volume 42 Nº 4 elocation e20190032

Resumo: En | Texto: En | PDF: En

Abstract Beta thalassemia (β-thal) is a frequent monogenic disease, is clinically and molecularly heterogeneous. This study described molecular and laboratory findings for three Mexican patients with β-thal intermedia phenotype and their relatives. Three Mexican families were studied for presenting β-thal intermedia, ARMS-PCR and Gap-PCR were performed to screen for common mutations, Sanger sequencing for rare or new alleles, and MLPA for identifying deletions and or duplications. In all three families we observed, in heterozygote condition, the mutation c.118C > T (p.Gln39*) also known as codon 39(C > T) in the β globin gene (HBB) associated with a novel molecular defect: a new duplication of the alpha globin gene cluster, a new deletion that includes the loss of exon 3 of HBB and finally a novel mutation in the 3’UTR of HBB (HBB: c.*132C > A). We report three Mexican families with beta thalassemia intermedia due to different molecular basis; a new single nucleotide mutation involving the last nucleotide of the β-globin chain transcript; and two possible new DNA rearrangements, an α cluster duplication, and a partial β gene deletion.

https://doi.org/10.1590/1678-4685-gmb-2019-0032 1162 downloads

Hemoglobina Fannin-Lubbock I y II, dos variantes que comparten una mutación
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Ibarra Cortés, Bertha

; Perea Díaz, Francisco Javier

; Rizo de la Torre, Lourdes del Carmen

Salud(i)Ciencia

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Mai 2017, Volume 22 Nº 5 Páginas 437 - 440

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Hemoglobin (Hb) Fannin-Lubbock I ( beta119 (GH2), GGC> GAC, Gly> Asp) and Hb Fannin-Lubbock II (beta111 (G13), GTC> CTC, Val> Leu and beta119 (GH2), GGC > GAC, Gly> Asp), share the mutation at position 119, both abnormal hemoglobins have similar fast electrophoretic mobility due the Gly> Asp change. Hb Fannin-Lubbock, is classified in the group of unstable hemoglobins, without alteration in their affinity for oxygen; the mutation at amino acid 119 and that of amino acid 111 participate in the interaction of the alpha1beta1 chains, with important links in the stability of the molecule, so that both substitutions could affect the stability of the molecule. Initially the instability of the variant was attributed to mutation 111, however, our group confirmed that the mutation at amino acid 119 is responsible for the instability to the molecule. In this paper, we analyze the first observations of Hb Fannin-Lubbock in 1976 and in 1982, the demonstration of Hb Fannin-Lubbock II by DNA sequencing as well as the evidence of two different mutations by DNA analysis, the Hb Fannin-Lubbock I observed mainly in families of Mexican origin and Hb Fannin-Lubbock II in families of Spanish origin, we also present the hematological characteristics of both types and the indirect evidence that the Hb Fanin-Lubbock I observed in a Mexican family and in a Hindu family are associated with haplotypes suggesting that they arose from independent mutational events.

Resumen La hemoglobina (Hb) Fannin-Lubbock I (beta119 (GH2), GGC>GAC, Gly>Asp) y la Hb Fannin-Lubbock II (beta111 (G13), GTC>CTC, Val>Leu y beta119 (GH2), GGC>GAC, Gly>Asp), comparten la mutación en la posición 119, tienen movilidad electroforética rápida similar debida al cambio Gly>Asp. La Hb Fannin-Lubbock se clasifica en el grupo de hemoglobinas inestables, sin alteración en su afinidad por el oxígeno; tanto la mutación en el aminoácido 119 como la del aminoácido 111 participan en la interacción de las cadenas alfa1beta1, por lo que ambas sustituciones podrían afectar la estabilidad de la molécula. Inicialmente la inestabilidad de la Hb se atribuyó a la mutación 111, sin embargo, nuestro grupo confirmó que la mutación en el aminoácido 119 es la responsable de la inestabilidad de la molécula. En este trabajo se analizan las primeras observaciones de la Hb Fannin-Lubbock en 1977 y en 1982, la demostración de la Hb Fannin-Lubbock II por secuenciación de ADN, así como la evidencia de dos mutaciones diferentes por análisis de ADN, la Hb Fannin-Lubbock I observada principalmente en familias de origen mexicano y la Hb Fannin-Lubbock II en familias de origen español, así como las características hematológicas de ambos tipos y la evidencia indirecta de que la Hb Fanin-Lubbock I observada en una familia mexicana y en una familia india se asocian con haplotipos beta distintos, lo que sugiere que surgieron por eventos mutacionales independientes.

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Seroprevalence and major antigens recognized by sera from Trypanosoma cruzi-infected dogs from Jalisco, México
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Martínez, Ignacio

; Martínez-Ibarra, Alejandro

; Arce-Fonseca, Minerva

; Rodríguez-Morales, Olivia

; Pérez-Morales, Deyanira

; Reyes López, Pedro A

; Espinoza, Bertha

Revista argentina de microbiología

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Jun 2014, Volume 46 Nº 2 Páginas 85 - 90

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Chagas disease is a major endemic disease caused by the protozoan parasite Trypanosoma cruzi. This parasitic disease is widely distributed throughout Latin America, affecting 10 million people. There are also reports of canine infection in the southern part of the United States. Dogs are considered the predominant domestic reservoir for T. cruzi in many areas of endemicity. In México, dog infection by this parasite has been poorly studied. In this work 209 dogs from six villages in Jalisco, México, were assessed to detect anti-T. cruzi antibodies by ELISA and Western blot. Seventeen (17) seropositive dogs (8.1 %) were detected by both tests, representing a seropositive value similar to that found in some southern states of México where the infection is present. No statistical differences were observed concerning the age and sex of infected and non-infected dogs. The major antigens recognized by positive sera were 26, 32, 66 and 80 kDa. These proteins are candidates to develop a specific diagnostic method for canine Chagas. No antibodies against HSP16 protein were found in T. cruzi seropositive sera. This is the first report of canine serology of Chagas disease in this central part of México. This report will contribute to the knowledge of the infection status of domestic reservoirs in the state of Jalisco, México.

El mal de Chagas es una enfermedad endémica causada por el parásito protozoario Trypanosoma cruzi. Este padecimiento está ampliamente distribuido en América, donde afecta a alrededor de 10 millones de personas. También existen comunicaciones de la infección canina desde el sur de los Estados Unidos hasta países de Sudamérica. Los perros son considerados los principales reservorios domésticos de T. cruzi en muchas áreas endémicas. En México, la infección canina ha sido estudiada escasamente. En el presente trabajo se evaluó mediante ELISA y Western blot la presencia de anticuerpos anti-T. cruzi en el suero de 209 perros de seis localidades del estado de Jalisco, México. Se encontraron 17 perros seropositivos (8,1 %) a ambas pruebas. No se observaron diferencias de significación estadística en la edad o el sexo de los perros infectados comparados con los no infectados. Los principales antígenos reconocidos por los sueros positivos fueron de 26, 32, 66 y 80 kDa. Estas proteínas son candidatos para desarrollar un método de diagnóstico específico para Chagas canino. No se encontraron anticuerpos contra la proteína HSP16 en los sueros positivos anti-T. cruzi. Este es el primer informe de serología canina en la región central de México y contribuirá al conocimiento de la infección en reservorios domésticos de Jalisco, México.

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Factores de riesgo para osteoporosis en mujeres posmenopáusicas de Guadalajara, Jalisco
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González-Mercado, Anahí

; Sánchez-López, J Yoaly

; Ibarra, Bertha

Salud Pública de México

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Dez 2013, Volume 55 Nº 6 Páginas 627 - 630

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Objective. To know risk factors and the frequency of osteoporosis (OP) in postmenopausal women. Materials and methods. Bone mineral density was measured in lumbar spine of 513 postmenopausal women from a hospital of Guadalajara, Jalisco during 2007-2008. The cutoff points of the associated variables were obtained by ROC curves and odds ratio (OR) by logistic regression. Results. The 25.2% (95%CI 21.44-28.96) of the women was OP. The variables associated with OP and cutoff points were age >60 years, weight <71kg, height <1.54m and BMI <29.2kg/m², with OR greater than 3.19 (p<0.0001). Conclusions. It is recommended setting cutoff points to estimate risk factors for OP more accurately in each population.

Objetivo. Conocer los factores de riesgo y la frecuencia de osteoporosis (OP) en mujeres posmenopáusicas. Material y métodos. Se midió la densidad mineral ósea de columna en 513 mujeres posmenopáusicas de un hospital de Guadalajara, Jalisco durante 2007-2008. Los puntos de corte de las variables asociadas se obtuvieron por curvas ROC y la razón de momios (RM) mediante regresión logística. Resultados. El 25.2% (IC95% 21.44-28.96) de las mujeres mostró OP. Las variables asociadas a OP y sus puntos de corte fueron: edad >60 años, peso <71kg, talla <1.54m e IMC <29.2kg/m², con RM mayores a 3.19 (p<0.0001). Conclusiones. Se recomienda establecer puntos de corte para estimar factores de riesgo para OP con mayor precisión en cada población.

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Factores de riesgo para osteoporosis en mujeres posmenopáusicas de Guadalajara, Jalisco
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González-Mercado, Anahí

; Sánchez-López, J Yoaly

; Ibarra, Bertha

Salud Pública de México

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Dez 2013, Volume 55 Nº 6 Páginas 627 - 630

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

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Relación entre la reacción al tizón común y el rendimiento y peso de la semilla de frijol en riego y temporal
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Acosta-Gallegos, Jorge A.

; Ibarra-Pérez, Francisco J.

; Navarrete-Maya, Rosa

; Sánchez-García, Bertha M.

; Jiménez Hernández, Yanet

; Mendoza Hernández, Francisco M.

Revista mexicana de ciencias agrícolas

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Ago 2013, Volume 4 Nº 5 Páginas 673 - 685

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Various diseases damage the bean crops in the central region of Mexico, especially those under rainfed conditions. The aim of this study was to identify bean genotypes tolerant to common blight (Xanthomonas campestris pv. phaseoli), and to determine the association between the reaction to this disease and yield and seed weight in 36 genotypes established under irrigation and rainfed conditions. From 2004 to 2006, an assay was established five times in Celaya, Guanajuato, with 36 genotypes of different origin and type of grain; two were conducted under irrigated conditions in February sowings, and three under rainfed conditions in July sowings. We determined the reaction ofthe genotypes to common blight incidence, their yield and the weight of 100 seeds of them. Overall, common blight incidence was observed in both planting systems at the beginning of the reproductive stage, intensifying, with differences between genotypes (p< 0.05), towards grain filling; the incidence was higher under rainfed irrigation. All genotypes showed, in varying degrees, disease symptoms on the leaves, and a few of them on the pods. No immune genotypes were observed; the ones with the lowest incidence ofthe disease, as shown in the assays, were: Afn, FEB 190, A 321 (reaction 2.3), Flor de Mayo Anita, PT 98001 (2.6), Alteño 2000, A 339, EMP507 and Flor de Mayo M38 (2.7). There was no relationship between the incidence of the disease and yield, but there was a relationship between the disease incidence and the weight of 100 seeds; this due to the susceptibility of the "pinto" and "bayo" genotypes with medium to large size seeds of the Durango race.

En la región centro de México diversas enfermedades dañan el cultivo del frijol, sobre todo bajo condiciones de temporal. El objetivo fue identificar genotipos de frijol tolerantes al tizón común (Xanthomonas campestris pv. phaseoli), determinar la asociación entre la reacción a esa enfermedad y el rendimiento y peso de la semilla en 36 genotipos establecidos bajo condiciones de riego y temporal. De 2004 a 2006, se estableció en Celaya, Guanajuato, en cinco ocasiones, un ensayo con 36 genotipos de diferente procedencia y tipo de grano, dos se condujeron bajo condiciones de riego en siembras de febrero y tres bajo temporal en siembras de julio. Se determinó la reacción de los genotipos a la incidencia de tizón común, el rendimiento y peso de 100 semillas. En general, en ambos sistemas de siembra la incidencia de tizón común se observó al inicio de la etapa reproductiva acentuándose, con diferencias entre genotipos (p< 0.05), hacia el llenado de grano; la incidencia fue mayor en temporal que en riego. Todos los genotipos mostraron, en diferente grado, síntomas de la enfermedad en hojas y pocos en las vainas. No se observaron genotipos inmunes, los de menor incidencia de la enfermedad a través de los ensayos fueron: Afi, FEB 190, A 321 (reacción 2.3), Flor de Mayo Anita, PT 98001 (2.6), Alteño 2000, A 339, EMP507 y Flor de Mayo M38 (2.7). No hubo relación entre la incidencia de la enfermedad y el rendimiento y si entre su incidencia y el peso de 100 semillas; esto debido a la susceptibilidad de genotipos de los tipos pinto y bayo con semilla de tamaño intermedio a grande de raza Durango.

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Analysis of the SLC4A1 gene in three Mexican patients with hereditary spherocytosis: report of a novel mutation
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Sánchez-López, Josefina Y.

; Camacho-Torres, Ana L.

; Ibarra, Bertha

; Tintos, Jesús A.

; Perea, Francisco J.

Genetics and Molecular Biology

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2010, Volume 33 Nº 1 Páginas 9 - 11

Resumo: En | Texto: En | PDF: En

We analyzed the SLC4A1 gene in three Mexican patients with Hereditary Spherocytosis (HS). The promoter and all 20 exons were investigated through heteroduplex analysis and DNA sequencing. No DNA changes were detected in one of the three patients. Two well-known polymorphisms, Memphis I and the Diego-a blood group, were detected in another one. In the third, the HS phenotype could be explained by the novel 1885_1888dupCCGG mutation found in heterozygosis. This frameshift mutation is predicted to result in a truncated and unstable protein lacking normal functions.

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Occurrence of hybrids and laboratory evidence of fertility among three species of the Phyllosoma complex (Hemiptera: Reduviidae) in Mexico
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Martínez-Ibarra, José Alejandro

; Salazar-Schettino, Paz María

; Nogueda-Torres, Benjamín

; Vences, Mauro Omar

; Tapia-González, José María

; Espinoza-Gutiérrez, Bertha

Memórias do Instituto Oswaldo Cruz

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Dez 2009, Volume 104 Nº 8 Páginas 1125 - 1131

Resumo: En | Texto: En | PDF: En

In seven studied communities of Western Mexico, triatomine specimens were sympatrically collected, some with atypical morphological characteristics in contrast to pure specimens, which were presumed to be hybrids. More than 200 specimens of Meccus pallidipennis and Meccus longipennis with brown-yellow markings on dorsal connexival segments were collected in Ahuacapán and Quitupan. In La Mesa, more than 60 specimens similar to Meccus picturatus in most morphological characteristics (including size) were collected, although they presented a largely yellowish corium like M. pallidipennis. Interfertility was proven between all of the studied wild hybrid specimens, as well as between all the experimental laboratory hybrids. Two different phenotypes (M. picturatus and M. longipennis) were obtained from crosses between M. picturatus x M. picturatus and M. longipennis x M. longipennis from the three studied localities in state of Nayarit as from La Mesita. Results support the hypothesis that the subspecific ranking of those triatomines may, therefore, be more appropriate because reproductive isolation has not been developed and complete interbreeding was recorded.

3145 downloads Citado 3 vezes em SciELO

10.

Análisis de los polimorfismos G199A, Ncol del gen ANK1 y Memphis I del gen SLC4A1 en Individuos sanos y pacientes mexicanos con esferocitosis hereditaria
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Camacho-Torres, Ana Luisa

; Sánchez-López, Josefina Yoaly

; Mesa-Cornejo, Viviana Matilde

; Ibarra, Bertha

; Javier Perea-Díaz, Francisco

Gaceta médica de México

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Out 2006, Volume 142 Nº 5 Páginas 435 - 437

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Background. In Mexico, Hereditary Spherocytosis (HS) is the main cause of hereditary hemolytic anemia, due to mutations of one or more genes involved in the erythrocyte membrane, making it difficult to identify the primary gene. Objective. With the purpose of estimating the use of the polymorphisms G199A and Ncol of ANKl gene, and Memphis I of SLC4A1 gene, as genetic markers to screen this disease, we searched the allelic and genotypic frequencies in 45 DNA samples of HS patients and 28 from healthy individuals. Results. Allelic and genotypic frequencies were similar in both studied groups for the G199A and Memphis I polymorphisms, with low frequency of heterozygosis showing its limited use as a genetic marker. The allelic and genotypic frequencies of the Ncol polymorphism were also similar in both groups, however a higher heterozygote frequency was observed (0.49 and 0.43 in patients and healthy individuals), a feature that may turn it into a useful genetic marker. Conclusions. Since there are other genes implicated in the molecular pathology of the HS, we consider it necessary to continue analyzing other polymorphisms of the genes involved in Hereditary Spherocytosis among the Mexican population,

Antecedentes. En México la esferocitosis hereditaria (EH) es la causa principal de anemia hemolítica hereditaria y se debe a mutaciones en uno o más genes implicados en la membrana eritrocitaria, lo que dificulta la identificación del gen primario. Objetivo. Con el fin de valorar la utilización de los polimorfismos G199A y Ncol del gen ANK1, y Memphis I del gen SLC4A1 como marcadores genéticos para identificar esta enfermedad estimamos sus frecuencias alélicas y genotípicas en 45 muestras de ADN de pacientes con EH y 28 de individuos sanos, las cuales fueron similares en uno y otro grupos para los polimorfismos G199A y Memphis I, con baja frecuencia de heterocigotos, lo que limita su utilidad como marcador genético. Resultados. El polimorfismo Ncol no mostró diferencias alélicas y genotípicas en los grupos de estudio, pero sí mayor frecuencia de heterocigotos (0.49 y 0.43 en enfermos y sanos respectivamente), caracteristica que le confiere ventajas para ser utilizado como marcador genético en familias conEH. Conclusiones. Finalmente, debido a que existen otros genes implicados en la patología molecular de la EH, consideramos que es necesario analizar otros polimorfismos de genes que codificanpara las proteínas involucradas en las deficiencias que conducen a esferocitosis hereditaria en la población mexicana.

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11.

Molecular characterization of the - -SEA alpha thalassemia allele in Mexican patients with HbH disease
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Nava, Maria Paulina

; Trejo, Jorge Martin

; Aguilar-Luna, Carlos

; Barros-Núñez, Patricio

; Chávez, Ma. de la Luz

; Magaña, María Teresa

; Perea, Javier

; Ibarra, Bertha

Revista de investigación clínica

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Ago 2006, Volume 58 Nº 4 Páginas 313 - 317

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

α-Thalassemia is one of the most prevalent hemoglobin disorders in the world, in South-East Asians, the--SEA allele is widely found in the HbH disease patients. The purpose of this work is to describe the molecular characteristics of Hemoglobin H disease in three patients from two Mexican families, as well to analyze the DNA sequence of the --SEA allele to determine the precise site of the crossover. The -α3.7 and --SEA alleles were identified using an established long-PCR method modified in our laboratory. The crossover site of --SEA mutation was analyzed by DNA sequencing. The three HbH subjects showed the same genotype -α3.7/--SEA. The -α3.7 allele has been observed in almost every racial studied group, whereas the --SEA allele is predominant in South-East Asian countries. DNA analysis through the breakpoint sites of the --SEA allele in both families showed the 5' breakpoint at the third base of codon 28 in the ψα2 gene and the 3' breakpoint within an Alu-Jo sequence, 1,328 nucleotides upstream of the 3'HVR. Therefore the size of the deletion is 19,303 nucleotides. This is the first report in which the flanking deletion sites of the--SEA mutation have been analyzed in Mexican patients, the 5' and 3' ends of the deletion is well determined.

La Talasemia-α es uno de los desórdenes de la hemoglobina más prevalences en el mundo. En el sureste de Asia, --SEA es el alelo más frecuente en pacientes con enfermedad por HbH (EHbH). En el presente trabajo se describen las características moleculares de tres pacientes con EHbH de dos familias mexicanas, y se analiza la secuencia de DNA del alelo --SEA, para determinar los sitios de ruptura. Los alelos -α3.7y --SEA se identificaron por un método de PCR modificado en nuestro laboratorio y los sitios de ruptura por secuenciación de DNA. Los tres pacientes con EHbH mostraron el genotipo -a3.7/--SEA. El alelo -α3.7 está ampliamente distribuido en el mundo, mientras que el alelo--SEA predomina en los países del sureste de Asia. El análisis de DNA del alelo--SEA mostró en 5' el sitio de ruptura en el codón 28 del pseudogén ψα2 y en 3', dentro de la secuencia Alu-Jo, localizada a 1,328 nucleótidos de la región HVR3', lo que da un segmento delecionado de 19,303 nucleótidos. Éste es el primer reporte en el que se analizan los sitios que flanquean la deleción del alelo --SEA en pacientes mexicanos y se definen con precisión los extremos 5' y 3' de la deleción.

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Por exemplo, se você deseja buscar artigos sobre
casos de dengue no Brasil em 2015, use:
ti:dengue and publication_year:2015 and aff_country:Brasil

Veja abaixo a lista completa de índices de pesquisa que podem ser usados:

Cód. do Índice	Elemento
ti	título do artigo
au	autor
kw	palavras-chave do artigo
subject	assunto (palavras do título, resumo e palavras-chave)
ab	resumo
ta	título abreviado da revista (ex. Cad. Saúde Pública)
journal_title	título completo da revista (ex. Cadernos de Saúde Pública)
la	código do idioma da publicação (ex. pt - Português, es - Espanhol)
type	tipo do documento
pid	identificador da publicação
publication_year	ano de publicação do artigo
sponsor	financiador
aff_country	código do país de afiliação do autor
aff_institution	instituição de afiliação do autor
volume	volume do artigo
issue	número do artigo
elocation	elocation
doi	número DOI
issn	ISSN da revista
in	código da coleção SciELO (ex. scl - Brasil, col - Colômbia)
use_license	código da licença de uso do artigo

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