Abstract Blumea balsamifera (Sembung) is a very famous ethnic medicinal herbs and functional tea in many countries of Asia. Phytochemical investigations showed that B. balsamifera contains various type chemical components, including volatile oil, flavonoids, terpenoids, phenylpropanoids, polysaccharides, etc. B. balsamifera has many pharmacological activities such as antioxidation, antibiosis, antitumor, liver protection, anti-inflammation, analgesia, and wound healing. This paper reviewed the chemical constituents and pharmacological activities of B. balsamifera for the further research, development, and utilization of B. balsamifera. Sembung (Sembung Asia B components oil flavonoids terpenoids phenylpropanoids polysaccharides etc antioxidation antibiosis antitumor protection antiinflammation, antiinflammation anti inflammation, inflammation anti-inflammation analgesia healing research development

Abstract: Blumea balsamifera is a very famous medicinal plant and functional tea in many countries of Asia. Ainaxiang tablets are a preparation consisting of flavonoids from B. balsamifera. Today, a high performance liquid chromatography method was developed for simultaneous quantification of 15 flavonoids in Ainaxiang Tablets. The analysis was performed on a Kromasil C18 column (250 × 4.6 mm, 5 µm) with a binary gradient mobile phase of acetonitrile and 0.2% aqueous acetic acid. The method was validated in terms of linearity, sensitivity, stability, precision and accuracy. It was found that this method had linearity with R2 at 0.9992- 0.9999 in the test range of 0.15-276.40 µg/mL. The limit of detection (LOD) and limit of quantification (LOQ) for 15 tested reference compounds were 0.01-0.28µg/mL and 0.02 - 0.55µg/mL, respectively. The relative standard deviations (RSD%) for intra-day and inter-day repeatability were not more than 3.18%. The analyzed samples were stable for at least 18 h. The spike recoveries for 15 analyzed samples were 106.17 - 95.02%. The results suggested that this newly developed HPLC method could be used for quantitative analysis of flavonoids in Ainaxiang Tablets and its raw materials. Abstract Asia B Today 1 C C1 250 (25 46 4 6 4. mm µm 02 0 2 0.2 acid sensitivity stability accuracy R 0.9992 09992 9992 09999 9999 0.999 0.15276.40 01527640 0.15 276.40 276 40 0.15-276.4 µgmL µg mL µg/mL LOD (LOD LOQ (LOQ 0.010.28µg/mL 001028µgmL 0.01 0.28µg/mL 01 28µg 002 0.0 055µgmL 55µg 0.55µg/mL respectively RSD% RSD (RSD% intraday intra day interday inter 318 3 3.18% h 10617 106 17 106.1 9502 95 95.02% materials 25 (2 0. 0999 999 0.99 15276 0.15276.4 0152764 015 0.1 27640 276.4 27 0.15-276. 010 001 028µgmL 00 (RSD 31 3.18 1061 10 106. 950 9 95.02 ( 099 99 0.9 1527 0.15276. 015276 2764 276. 0.15-276 3.1 95.0 09 152 0.15276 01527 0.15-27 3. 95. 0.1527 0152 0.15-2 0.152 0.15-

RESUMO O presente trabalho visa investigar os efeitos do extrato de semente de uva Proanthocyanidin (GSPE) sobre o desempenho da produção, o metabolismo e o status antioxidante de gado leiteiro Holstein em lactação precoce. Quarenta e oito vacas leiteiras multíparas Holstein foram divididas em quatro grupos (CON, G20, G40 e G80) e receberam 0, 20, 40 e 80mg de GSPE/kg de peso corporal/dia, respectivamente. O G20 aumentou significativamente o rendimento do leite em comparação com os outros grupos. A proteína e os sólidos não gordurosos do leite foram aumentados nos grupos G20, G40 e G80 somente no sétimo dia durante a experiência. Não foi observada diferença significativa na gordura do leite e na contagem de células somáticas, bem como nos parâmetros de metabolismo energético no sangue, na função hepática e na função renal entre os grupos em relação ao grupo controle. Não houve diferença significativa na glutationa peroxidase, na dimutase de superóxido, na capacidade antioxidante total e no peróxido de hidrogênio entre os grupos, mas o conteúdo malondialdeído do G20 aumentou significativamente no dia 14 em comparação com o CON, e tendia a aumentar no dia 28. Em conclusão, a alimentação de 20mg de GSPE/kg de peso corporal/dia foi associada a um aumento significativo no rendimento do leite, sem efeitos nocivos sobre a função hepática ou a renal, com o metabolismo de energia substancial e a melhoria dos parâmetros antioxidantes de gado leiteiro no início da lactação.

ABSTRACT Aims to investigate the effects of grape seed proanthocyanidin extract (GSPE) on production performance, metabolism, and anti-oxidative status of Holstein dairy cattle in early lactation. Forty-eight multiparous Holstein dairy cattle were assigned to four groups (CON, G20, G40 and G80) and supplied with 0, 20, 40, and 80mg GSPE/kg of body weight/day. G20 significantly increased milk yield compared with other groups. Milk protein and non-fat-solids were increased in G20, G40 and G80 groups compared with the control group only at the 7th day during the experiment. No significant difference was observed in milk fat and somatic cell count, nor on parameters of energy metabolism in blood, liver function and kidney function between the four groups. There was no significant difference in glutathione peroxidase, superoxide dismutase, total antioxidant capacity, and hydrogen peroxide between the groups; but the malondialdehyde content of G20 significantly increased at day 14 in comparison with CON, and tended to increase at the 28th day. In conclusion, feeding 20mg GSPE/kg of body weight/day was associated with a significant increase in milk yield without detrimental effects on liver or kidney function and with substantial energy metabolism and antioxidant parameters improvement in early lactation dairy cattle.

RESUMO Objetivo: Uma revisão retrospectiva tem como objetivo investigar os métodos operatórios e efeitos terapêuticos da abordagem modificada de Stoppa para o tratamento de fraturas pélvicas e acetabulares. Métodos: 18 pacientes com fratura acetabular da coluna anterior e fratura do anel anterior pélvico foram submetidos a tratamento cirúrgico utilizando a abordagem de Stoppa modificada. Parte do tratamento foi feita em conjunto com a abordagem da fossa ilíaca ou com a abordagem pelo acesso posterior de K-L. A redução da fratura e a função pós-operatória foram avaliadas pelo padrão de pontuação de Matta e o sistema de pontuação de Majeed. O sistema do Oxford Centre for Evidence-Based Medicine foi usado para classificar a revisão de literatura e criar as recomendações de grau B. Resultados: O comprimento da incisão foi de 6 a 12 cm (média de 10 cm), a duração da cirurgia foi de 50 a 150 minutos (média de 85 minutos) e o volume de perda sanguínea intraoperatória foi de 400 a 1.000 ml (média de 500 ml). 18 pacientes foram acompanhados por 12-36 meses após a operação. Nos resultados dos filmes radiográficos, 12 casos foram de reduções anatômicas e os demais casos foram de reduções satisfatórias. De acordo com o padrão de Majeed, 13 pacientes foram considerados excelentes e cinco pacientes foram considerados bons. Conclusões: O tratamento que utilizou a abordagem de Stoppa modificada foi adequado para abordagens anteriores, nas quais as fraturas pélvicas e acetabulares estavam suficientemente expostas, a fratura foi convenientemente reduzida, ocorreram menos complicações e o efeito curativo foi satisfatório. Nível de evidencia III, Estudo retrospectivo comparativo.

ABSTRACT Objective: A retrospective review aims to investigate the operative methods and therapeutic effects of the modified Stoppa approach for treating pelvic and acetabular fractures. Methods: 18 patients with acetabular fracture of the anterior column and pelvic anterior ring fracture underwent surgical treatment using the modified Stoppa approach. Some of the treatment was combined with the iliac fossa approach or rear K-L approach. Fracture reduction and postoperative function were evaluated using the Matta scoring standard and the Majeed scoring system. The Oxford Centre for Evidence-Based Medicine system was used to grade the literature review and create graded B recommendations. Results: Incision length was 6-12 cm (mean, 10 cm), operative duration was 50-150 minutes (mean, 85 minutes), and intraoperative blood loss volume was 400-1,000 ml (mean, 500 ml). 18 patients were followed up for 12-36 months post-operation. In the results of X-ray films, 12 cases were anatomical reductions and the remaining cases were satisfactory reductions. According to Majeed standard, 13 patients were excellent and five patients were good. Conclusions: Treatment using the modified Stoppa approach was suitable for anterior approaches, in which pelvic and acetabular fractures were sufficiently exposed, the fracture was conveniently reduced, less complications occurred, and curative effect was satisfactory. Level of evidence III, Retrospective comparative study.

Cocoa has experienced significant growth in recent years in Peru and the presence of heavy metals in the soils of these plantations is a potential problem for the export of this product. The objective of the present study was to determine the total concentrations of heavy metals (Cd, Ni, Pb, Fe, Cu, Zn, Mn) in soils with cacao crops in the main productive areas in Peru: North (Tumbes, Piura, Cajamarca and Amazonas), Center (San Martin, Huánuco and Junín) and South (Cuzco). Contents of heavy metals in soils of cocoa plantations from 10 to 15 years old were evaluated. Physical (texture) and chemical (pH, organic matter, CEC, P, K, Ca, Mg, Al, Cd, Ni, Pb, Fe, Cu, Zn, Mn) analyzes were conducted in the soils. The results show favorable growing conditions for cocoa. The values found for heavy metals were below those considered as phytotoxic. Mean values of total Fe, Zn, Mn, Ni and Pb were higher in the southern region while in the northern region the values of Cu and Cd were higher. In general, pH, % of Clay and Mg were the variables that had higher significant correlations with heavy metals.

El cacao ha tenido un crecimiento significativo en los últimos años en el Perú y la presencia de metales pesados en los suelos de estas plantaciones es un potencial problema para las exportaciones de este producto. El objetivo del presente estudio fue determinar los contenidos totales de metales pesados (Cd, Ni, Pb, Fe, Cu, Zn, Mn) en los suelos de plantaciones de cacao en las principales áreas de producción del Perú: Zona Norte (Tumbes, Piura, Cajamarca y Amazonas); Zona central (San Martín, Huánuco y Junín); Zona Sur (Cuzco). Se consideraron plantaciones entre 10 y 15 años de edad. Se realizaron los análisis físicos (textura) y químicos (pH, materia orgánica, CIC, P, K, Ca, Mg, Al, Cd, Ni, Pb, Fe, Cu, Zn, Mn) de los suelos muestreados. Los suelos en el estudio presentan adecuadas condiciones físicas y químicas para el cultivo de cacao. Los valores de metales pesados se encontraron por debajo de lo considerado como fitotóxico. Los valores promedio de hierro, zinc, manganeso, níquel y plomo fueron mayores en la zona sur, mientras que en la zona norte los valores de cobre y cadmio fueron mayores. De forma general el pH, % de arcilla y Mg fueron las variables que tuvieron mayor correlación con la concentración de metales pesados.

ABSTRACT Objective To evaluate the clinical efficiency of alpha1-adrenergic antagonists on stentless ureteroscopic lithotripsy treating uncomplicated lower ureteral stones. Materials and Methods From January 2007 to January 2013, 84 patients who have uncomplicated lower ureteral stones treated by ureteroscopic intracorporeal lithotripsy with the holmium laser were analyzed. The patients were divided into two groups, group A (44 patients received indwelled double-J stents) and group B (40 patients were treated by alpha1-adrenergic antagonists without stents). All cases of group B were treated with alpha1 blocker for 1 week. Results The mean operative time of group A was significantly longer than group B. The incidences of hematuria, flank/abdominal pain, frequency/urgency after surgery were statistically different between both groups. The stone-free rate of each group was 100%. Conclusions The effect of alpha1-adrenergic antagonists is more significant than indwelling stent after ureteroscopic lithotripsy in treating uncomplicated lower ureteral stones.

Magnesium, a promising biodegradable metal, has been reported in several studies to increase bone formation. Although there is some information regarding the concentrations of magnesium ions that affect bone remodeling at a cellular level, little is known about the effect of magnesium ions on cell gap junctions. Therefore, this study aimed to systematically investigate the effects of different concentrations of magnesium on bone cells, and further evaluate its effect on gap junctions of osteoblasts. Cultures of normal human osteoblasts were treated with magnesium ions at concentrations of 1, 2 and 3 mM, for 24, 48 and 72 h. The effects of magnesium ions on viability and function of normal human osteoblasts and on gap junction intercellular communication (GJIC) in osteoblasts were investigated. Magnesium ions induced significant (P<0.05) increases in cell viability, alkaline phosphate activity and osteocalcin levels of human osteoblasts. These stimulatory actions were positively associated with the concentration of magnesium and the time of exposure. Furthermore, the GJIC of osteoblasts was significantly promoted by magnesium ions. In conclusion, this study demonstrated that magnesium ions induced the activity of osteoblasts by enhancing GJIC between cells, and influenced bone formation. These findings may contribute to a better understanding of the influence of magnesium on bone remodeling and to the advance of its application in clinical practice.

This study aimed to evaluate the long-term survival and risk factors of traditional open surgical repair (OSR) vs thoracic endovascular aneurysm repair (TEVAR) for complicated type-B aortic dissection (TBAD). A total of 118 inpatients (45 OSR vs 73 TEVAR) with TBAD were enrolled from January 2004 to January 2015. Kaplan-Meier curves and Cox proportional hazards analysis were performed to identify the long-term survival rate and independent predictors of survival, respectively. Meta-analysis was used to further explore the long-term efficacy of OSR and TEVAR in the eight included studies using Review Manager 5.2 software. An overall 10-year survival rate of 41.9% was found, and it was similar in the two groups (56.7% OSR vs 26.1% TEVAR; log-rank P=0.953). The risk factors of long-term survival were refractory hypertension (OR=11.1; 95%CI=1.428-86.372; P=0.021] and preoperative aortic diameter >55 mm (OR=4.5; 95%CI=1.842-11.346; P=0.001). Long-term survival rate did not differ significantly between OSR and TEVAR (hazard ratio=0.87; 95%CI=0.52-1.47; P=0.61). Compared with OSR, TEVAR did not show long-term advantages for patients with TBAD. Refractory hypertension and total aortic diameter >55 mm can be used to predict the long-term survival of TBAD in the Chinese Han population.

Biomineralization is a known natural phenomenon associated with a wide range of bacterial species. Bacterial-induced calcium carbonate precipitation by marine isolates was investigated in this study. Three genera of ureolytic bacteria, Sporosarcina sp., Bacillus sp. and Brevundimonas sp. were observed to precipitate calcium carbonate minerals. Of these species, Sporosarcina sp. dominated the cultured isolates. B. lentus CP28 generated higher urease activity and facilitated more efficient precipitation of calcium carbonate at 3.24 ± 0.25 × 10−4 mg/cell. X-ray diffraction indicated that the dominant calcium carbonate phase was calcite. Scanning electron microscopy showed that morphologies of the minerals were dominated by cubic, rhombic and polygonal plate-like crystals. The dynamic process of microbial calcium carbonate precipitation revealed that B. lentus CP28 precipitated calcite crystals through the enzymatic hydrolysis of urea, and that when ammonium ion concentrations reached 746 mM and the pH reached 9.6, that favored calcite precipitation at a higher level of 96 mg/L. The results of this research provide evidence that a variety of marine bacteria can induce calcium carbonate precipitation, and may influence the marine carbonate cycle in natural environments.

O fornecimento de P para as culturas em solos tropicais é deficiente em razão da sua natureza pouco solúvel no solo; e a adição de fertilizantes fosfatados é necessária para obter-se alta produtividade. O objetivo deste estudo foi examinar os mecanismos pelos quais uma planta de cobertura (Braquiária - Brachiaria ruziziensis) cultivada em rotação com soja pode melhorar a disponibilidade do P de fertilizantes e do solo, utilizando ensaios de campo de longo prazo e abordagens de laboratório com fracionamento químico do P. O campo experimental vinha sendo cultivado com soja em rotação com diversas espécies sob semeadura direta há seis anos. Doses de 0 ou 240 kg ha-1 de P2O5, como superfosfato triplo ou fosfato reativo de Arad, haviam sido aplicadas. Em abril de 2009, foram aplicados novamente 0 e 80 kg ha-1 de P2O5, às mesmas parcelas, quando foi semeada B. ruziziensis. Em novembro de 2009, após a dessecação da braquiária, amostras de solo foram coletadas nas camadas de 0-5 e 5-10 cm, e o P foi fracionado. O P disponível do solo aumentou em profundidade com o cultivo de B. ruziziensis na presença de fertilizantes fosfatados. A relação C/P também foi aumentada pela planta de cobertura. O cultivo de B. ruziziensis elevou os teores de P na fração húmica do solo. A B. ruziziensis aumenta a disponibilidade de P no solo por prevenir a fixação do P aplicado como fertilizante e manter o P em formas orgânicas.

The phosphorus supply to crops in tropical soils is deficient due to its somewhat insoluble nature in soil, and addition of P fertilizers has been necessary to achieve high yields. The objective of this study was to examine the mechanisms through which a cover crop (Congo grass - Brachiaria ruziziensis) in rotation with soybean can enhance soil and fertilizer P availability using long-term field trials and laboratory chemical fractionation approaches. The experimental field had been cropped to soybean in rotation with several species under no-till for six years. An application rate of no P or 240 kg ha-1 of P2O5 had been applied as triple superphosphate or as Arad rock phosphate. In April 2009, once more 0.0 or 80.0 kg ha-1 of P2O5 was applied to the same plots when Congo grass was planted. In November 2009, after Congo grass desiccation, soil samples were taken from the 0-5 and 5-10 cm depth layer and soil P was fractionated. Soil-available P increased to the depth of 10 cm through growing Congo grass when P fertilizers were applied. The C:P ratio was also increased by the cover crop. Congo grass cultivation increased P content in the soil humic fraction to the depth of 10 cm. Congo grass increases soil P availability by preventing fertilizer from being adsorbed and by increasing soil organic P.

PURPOSE: Cerebral vasospasm (CVS) is a major complication after subarachnoid hemorrhage (SAH) induced by the rupture of intracranial aneurysms. The aim of the present study was to investigate the effect and mechanism of cervical sympathetic block on cerebral vasospasm of the rabbits after SAH. METHODS: After successful modeling of cervical sympathetic block, 18 healthy male white rabbits were randomly divided into three groups (n=6), ie, sham operation group (Group A), SAH group (Group B) and SAH with cervical sympathetic block group (Group C). Models of delayed CVS were established by puncturing cisterna magna twice with an injection of autologous arterial blood in Groups B and C. A sham injection of blood through cisterna magna was made in Group A. 0.5 ml saline was injected each time through a catheter for cervical sympathetic block after the first injection of blood three times a day for 3 d in Group B (bilateral alternating). 0.5 ml of 0.25% bupivacaine was injected each time through a catheter for cervical sympathetic block after the first injection of blood three times a day for 7 d in Group B. 2 ml venous blood and cerebrospinal fluid were obtained before (T1), 30 min (T2) and 7 d (T3) after the first injection of blood, respectively, and conserved in a low temperature refrigerator. Basilar artery value at T1, T2 and T3 was measured via cerebral angiography. The degree of damage to nervous system at T1 and T3 was recorded. RESULTS: There was no significant difference in diameter of basilar artery at T1 among three groups. The diameters of basilar artery at T2 and T3 of Groups B and C were all smaller than that in Group A, which was smaller than Group C, with a significant difference. There was no significant difference in NO and NOS in plasma and cerebrospinal fluid among three groups. The NO and NOS contents at T2 and T3 of Groups B and C were all lower than Group A; Group C was higher than Group B, with a significant difference. The nerve function at T3 of Groups B and C were all lower than Group A and that of Group C higher than Group B, with a significant difference. CONCLUSION: Cervical sympathetic block can relieve cerebral vasospasm after subarachnoid hemorrhage and increase NO content and NOS activity in plasma and cerebrospinal fluid to promote neural functional recovery.

OBJETIVO: Investigar os efeitos da destruição da raiz dorsal ganglionar em pacientes com neuralgia pós-herpética. MÉTODOS: Setenta e dois pacientes selecionados com neuralgia pós-herpética foram randomicamente distribuídos em dois grupos (n=36). Grupo A foi o grupo controle (tratado por injeção) e o grupo B foi o grupo com destruição da raiz dorsal do gânglio pela adriamicina. Os escores da Escala Analógica Visual (VAS), SAS, SF-MPQ escores, efeitos clínicos e segurança terapêutica foram avaliados as antes da terapia, uma semana, três e seis meses após a terapia. Quarenta e quatro pacientes foram avaliados pela análise de intenção-em-tratar. RESULTADOS: A média dos escores de dor na escala de Likert foi significativamente reduzida em cada ponto no grupo B. Pacientes no grupo B relataram efetividade clínica aos seis meses com excelente resposta (16), boa resposta (12), melhora mais insatisfatória ou sem modificações (8). Escores VAS a cada tempo após o procedimento foram melhores em comparação ao pré-operatório. No grupo A não foi observada diferença significativa. Pacientes mostraram melhora nos escores de dormir no grupo B. Houve diferença significante no T2 no grupo A que T1. Não houve diferença significante no grupo A nos tempos T3 e T4 após a cirurgia em relação a antes. Comparação entre os grupos: houve diferença significante entre os grupos A e B a cada tempo após a cirurgia. CONCLUSÕES: A destruição da raiz dorsal ganglionar pela adriamicina sob perspectiva guiada pelo C-arm, a cirurgia pontual foi acurada sem qualquer reação adversa ou complicação séria, que pode efetivamente aliviar a dor em pacientes com neuralgia pós-herpética, mas os efeitos de longo prazo necessitam mais estudos.

PURPOSE: To investigate the effects of dorsal root ganglion destruction in patients with postherpetic neuralgia (PHN). METHODS: Seventy-two patients with PHN selected were randomly divided into two groups (n=36). Group A was the control group (treated by injection) and group B was the group of dorsal root ganglion destruction by adriamycin. Visual analog scale scores (VAS), SAS, SF-MPQ scores. Clinical effects and therapy safety were evaluated before therapy, one week, three and six months after therapy. Forty-four patients were available for intention-to-treat analysis. RESULTS: The average pain scores on the Likert scale were significantly reduced at each point in group B. Patients in group B reported clinical effectiveness at six months as excellent response, good response, improved but unsatisfactory or unchanged 16, 12 and 8.VAS scores at each time point after the operation were lower than that before operation and in group A, there was significant difference. Patients showed significant improvement in sleep scores in group B. There was significant difference at T2 in group A than T1. There was no significant difference in group A at T3, T4 after the operation than that before operation. Between group comparison: there was significant difference between group A and group B at each time point after the operation. CONCLUSIONS: Dorsal root ganglion destruction by adriamycin under guidance of C-arm perspective, the puncture operation was accurate without any adverse reaction or serious complications, which could effectively relieve pain of patients with postherpetic neuralgia, but the long-term effects needed further study.

Foram moídas duas séries de vidros silicatos para a obtenção de partículas com dimensões nas faixas micrométrica, submicrométrica e nanométrica, utilizando três tipos distintos de processos de moagem: moagem em moinho de esferas, em atritor, e de alta energia. Foram investigados os efeitos do tempo e do tipo do meio de moagem no tamanho de partícula, em suspensões aquosas e em isopropanol. Foram utilizados um granulômetro com o princípio de difração de laser e um microscópio eletrônico de varredura para a determinação do tamanho de partícula. A distribuição de tamanho de partículas com o menor tamanho médio de partícula igual a 0,3 µm foi obtida para vidros submetidos ao processo de cominuição em duas etapas, utilizando um moinho de alta energia.

Two series of silicate glasses were processed to micron-size, sub-micron size, and nanoparticles using three different milling systems: ball milling, attrition, and high-energy milling. The effect of milling time and media size on particle size and contamination were investigated in aqueous and isopropanol suspensions. The particle size was determined using a laser-diffraction particle size analyzer and scanning electron microscopy. The smallest glass particles with a median particle size of 0.3 µm were achieved by a two-step comminution process in a high energy mill.