Resumo Objetivo Para pacientes idosos, a endoscopia gastrointestinal indolor pode facilmente induzir eventos adversos. Nosso objetivo foi avaliar os efeitos de intervenções de enfermagem de alta qualidade nos resultados e na taxa de complicações de pacientes idosos submetidos à endoscopia gastrointestinal indolor. Métodos Foram selecionados 124 pacientes que receberam endoscopia gastrointestinal indolor no período de janeiro a dezembro de 2022, 62 dos quais internados entre janeiro e junho de 2022 foram incluídos em um grupo de enfermagem convencional e receberam intervenção de enfermagem básica tradicional, e os outros 62 internados entre julho e dezembro 2022 foram atribuídos a um grupo de enfermagem de alta qualidade e receberam intervenções de enfermagem de alta qualidade. Os resultados da intervenção foram comparados. Resultados As pontuações da Escala de Autoavaliação de Ansiedade e da Escala de Autoavaliação de Depressão do grupo de enfermagem de alta qualidade foram inferiores às do grupo de enfermagem convencional (P<0,05). A pressão arterial sistólica, a pressão arterial diastólica e a frequência cardíaca durante e após o diagnóstico e tratamento diminuíram no grupo de enfermagem de alta qualidade em comparação com os do grupo de enfermagem convencional (P <0,05). O grupo de enfermagem de alta qualidade teve uma taxa de complicação mais baixa e uma taxa de satisfação de enfermagem mais alta do que o grupo de enfermagem convencional (P<0,05). Conclusão A intervenção de enfermagem de alta qualidade não só estabiliza o estado psicológico dos pacientes idosos submetidos à endoscopia gastrointestinal indolor, mas também é benéfica para manter a pressão arterial e a frequência cardíaca estáveis. adversos 12 6 202 tradicional comparados P<0,05. P005 P P<0,05 . 0 05 (P<0,05) sistólica <0,05. 005 <0,05 <0,05) estáveis 1 20 P00 P<0,0 (P<0,05 00 <0,0 2 P0 P<0, (P<0,0 <0, P<0 (P<0, <0 P< (P<0 < (P<

Resumen Objetivo En pacientes mayores, la endoscopía gastrointestinal indolora puede inducir eventos adversos fácilmente. Nuestro objetivo fue evaluar los efectos de intervenciones de enfermería de alta calidad en los resultados y en el índice de complicaciones de pacientes mayores sometidos a endoscopía gastrointestinal indolora. Métodos Se seleccionaron 124 pacientes que recibieron endoscopía gastrointestinal indolora en el período de enero a diciembre de 2022. Entre ellos, se incluyó a 62 pacientes internados entre enero y junio de 2022 en un grupo de enfermería convencional y recibieron intervenciones de enfermería básica tradicional. Los otros 62 internados entre julio y diciembre de 2022 fueron asignados a un grupo de enfermería de alta calidad y recibieron intervenciones de enfermería de alta calidad. Se compararon los resultados de la intervención. Resultados Los puntajes de la Escala de Autoevaluación de Ansiedad y de la Escala de Autoevaluación de Depresión del grupo de enfermería de alta calidad fueron inferiores a los del grupo de enfermería convencional (p<0,05). La presión arterial sistólica, la presión arterial diastólica y la frecuencia cardíaca durante y después del diagnóstico y tratamiento disminuyeron en el grupo de enfermería de alta calidad en comparación con el grupo de enfermería convencional (p<0,05). El grupo de enfermería de alta calidad tuvo un índice de complicaciones más bajo y un índice de satisfacción de enfermería más alto que el grupo de enfermería convencional (p<0,05). Conclusión La intervención de enfermería de alta calidad no solo estabiliza el estado psicológico de los pacientes mayores sometidos a endoscopía gastrointestinal indolora, sino que también es benéfica para mantener la presión arterial y la frecuencia cardíaca estables. fácilmente 12 ellos 6 202 tradicional p<0,05. p005 p p<0,05 . 0 05 (p<0,05) sistólica estables 1 20 p00 p<0,0 (p<0,05 2 p0 p<0, (p<0,0 p<0 (p<0, p< (p<0 (p< (p

Abstract Objective For elderly patients, painless gastrointestinal endoscopy can easily induce adverse events. We aimed to evaluate the effects of high-quality nursing interventions on the outcomes and complication rate of elderly patients undergoing painless gastrointestinal endoscopy. Methods A total of 124 patients who received painless gastrointestinal endoscopy from January to December 2022 were selected, 62 of whom admitted between January and June 2022 were included into a conventional nursing group and given traditional basic nursing intervention, and the other 62 admitted between July and December 2022 were assigned into a high-quality nursing group and given high-quality nursing intervention. The intervention outcomes were compared. Results The Self-rating Anxiety Scale and Self-rating Depression Scale scores of the high-quality nursing group were lower than those of the conventional nursing group (P<0.05). The systolic blood pressure, diastolic blood pressure, and heart rate during and after diagnosis and treatment decreased in the high-quality nursing group compared with those in the conventional nursing group (P<0.05). The high-quality nursing group had a lower complication rate and a higher nursing satisfaction rate than those of the conventional nursing group (P<0.05). Conclusion High-quality nursing intervention not only stabilizes the psychological state of elderly patients undergoing painless gastrointestinal endoscopy, but also is beneficial to maintaining stable blood pressure and heart rate. events highquality high quality 12 202 selected 6 Selfrating Self rating P<0.05. P005 P P<0.05 . 0 05 (P<0.05) Highquality High 1 20 P00 P<0.0 (P<0.05 2 P0 P<0. (P<0.0 P<0 (P<0. P< (P<0 (P< (P

Abstract Serum uric acid (UA) is a traditional biomarker in the clinical diagnosis of gout and hyperuricemia. However, serum treatment and storage are cumbersome, and wounds are susceptible to infection. Therefore, in this study, a simple and noninvasive method was developed to detect the UA in human saliva to monitor the gout. An Inertsil ODS-3 column was used for the analysis under the condition of isocratic elution with the mixed solution phosphate buffer (74 mM, pH=2.2): Methanol=98:2 (v:v) and the UV detection at 284 nm. Using salivary UA data from healthy volunteers (HVs) (n=68) and gout patients (GPs) (n=14), we examined the salivary UA difference in their content. The intra-and inter-day accuracy and precision (RSD %) were less than 2.56%, the limit of detection (LOD) of UA was 5.0 ng/mL, the mean recoveries of the corresponding compounds were 102.48%. Saliva levels of UA in HVs and GPs were 35.26±14.06 µg/mL and 91.96±23.90 µg/mL, respectively. The concentrations of salivary UA in GPs were significantly higher than those in HVs ( p < 0.001). This method was also expected to monitor the hyperuricemia and other metabolic disorders in the future. (UA However cumbersome infection Therefore study ODS3 ODS 3 ODS- 74 (7 mM pH=2.2 pH22 pH 2 pH=2.2) Methanol=982 Methanol982 Methanol Methanol=98 98 Methanol=98: vv v (v:v 28 nm (HVs n=68 n68 n 68 (n=68 (GPs n=14, n14 n=14 , 14 (n=14) content intraand intra interday inter day RSD % 256 56 2.56% LOD (LOD 50 5 0 5. ngmL ng mL ng/mL 10248 102 48 102.48% 35261406 35 26 06 35.26±14.0 µgmL µg 91962390 91 96 23 90 91.96±23.9 respectively 0.001. 0001 0.001 . 001 0.001) future 7 pH=2. pH2 982 Methanol98 Methanol=9 9 n=6 n6 6 (n=6 n1 n=1 1 (n=14 25 2.56 1024 10 4 102.48 3526140 35.26±14. 9196239 91.96±23. 000 0.00 00 pH=2 Methanol9 Methanol= n= (n= (n=1 2.5 102.4 352614 35.26±14 919623 91.96±23 0.0 pH= (n 2. 102. 35261 35.26±1 91962 91.96±2 0. 3526 35.26± 9196 91.96± 352 35.26 919 91.96 35.2 91.9 35. 91.

A series of metal ion (M = Sn2+, Fe3+, Ni2+, Co2+, Ag+, Cu2+) exchanged tungstophosphoric acid (H3PW12O40; TPA) catalysts with tunable Brønsted/Lewis acidity were synthesized and exploited for the oxidation of benzyl alcohol (BzOH) to benzaldehyde (BzH) using hydrogen peroxide (H2O2) as oxidant. The structure of these M-TPA composite salts was also characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analyses (TGA) and solid-state 31P nuclear magnetic resonance (NMR) probe molecule method. Among these M-TPAs, the Sn1/2H2PW12O40 catalyst, which presented strong Brønsted acidity, the synergistic effect of Brønsted/Lewis and pseudo-liquid characteristic property, exhibited excellent catalytic activity and durability with 98.2% of BzH selectivity and 95.1% of BzH yield. The optimal conditions for the oxidation of BzOH optimized by response surface methodology (RSM) were as follows: n(BzOH)/n(H2O2) = 1:1.25, catalyst amount of 5.5 wt.% to BzOH, water amount of 17 mL, 3.3 h of reaction time, and temperature 393 K. Moreover, further kinetic study confirmed that the reaction order was 2.64 and the activation energy was 21.75 kJ mol-1.

The detection of vitamin D in complex samples is challenging because of the instability of vitamin D in sample preparation. To resolve this problem, packed-fiber solid-phase extraction (PFSPE) has been successfully developed for the pre-treatment of samples containing vitamin D2 (D2) and vitamin D3 (D3) in dairy products. Based on PFSPE, vitamin D could avoid oxidation because of the shorter extraction time. The adsorbent materials, polystyrene/polypyrrole (PS/PPy) nanofibers, were prepared by electrospinning of PS and in situ polymerization of PPy on the surface of PS nanofibers. The linearity was within the range of 1-2000 ng mL−1 in dairy solution, and the recoveries were 91.9-109.1% with a relative standard deviation (RSD) < 13.6%. Therefore, the prepared method could be used for the detection of D2 and D3 in dairy products.

A series of N-aryl-5,6,7,8-tetra-hydrobenzo[4,5]thieno[2,3-d ]pyrimidin-4-amines were synthesized in moderate to good yield by using a microwave-enhanced conditions. The selected compounds were evaluated for their cytotoxic effects (IC50 values) on human pulmonary carcinoma (A549), murine BALB/c spontaneous colon adenocarcinoma (CT26) and human hepatocellular liver carcinoma (HepG2) cell lines in vitro. Amongst these compounds, one compound was found to have the better cytotoxic activity with reference to the standard Erlotinib hydrochloride (Tarceva™) against A549 (IC50 = 16.06 ± 0.09 µM) and HepG2 (IC50 = 15.01 ± 0.31 µM) cell lines. Especially, two compounds showed best cytotoxic effects against CT26 (IC50 = 11.38 ± 0.44 µM) and HepG2 (IC50 = 8.51 ± 0.52 µM) cell lines, respectively. The preliminary structure-activity relationships were disclosed and the thieno[2,3-d] pyrimidine skeleton could be exploited to potential antitumor agents in the future.

Although Agrobacterium-mediated transformation protocols for many economically important plant species have been well established, protocol for a number of flowering plants including Anthurium andraeanum remains challenging. In this study, we report success in generating transgenic Anthurium andraeanum cv Arizona using Agrobacterium GV3101 strain harboring a binary vector carrying gfp as a reporter gene. The possibility of facilitating the screening process for transgenic plants expressing functional proteins using gfp marker was explored. In order to realize high transformation efficiency, different explant sources including undifferentiated callus pieces and petioles were compared for their regeneration efficiency and susceptibility to Agrobacterium-mediated transformation. We also optimized the concentration of AS added to co-cultivation media. Genomic PCR revealed that 11 of the 22 resistant plantlets regenerated on selective medium were successfully transformed. Green fluorescence was observed using a fluorescence microscope in 7 of the 11 PCR-positive plants, indicating GFP was expressed stably in the transformed Anthurium andraeanum. The highest transformation efficiency obtained in this study was 1.71% (percentage of explants with transgenic shoots in total explants) when callus explants were used as starting material and 125 μmol l-1 AS was added during the co-cultivation process.

Apoptosis-inducing factor (AIF) is a mitochondrial flavoprotein that mediates both NADH-oxidizing and caspase-independent apoptosis. Further, the proapoptotic activity of AIF is located in the C-terminus of AIF, although the precise minimum sequence responsible for apoptosis induction remains to be investigated. In the present study, we generated two truncated AIFs, AIFΔ1-480-FLAG, which is a FLAG-tagged C-terminal peptide comprising amino acids from 481 to 613, and AIF360-480 containing amino acids from 360 to 480 of AIF. We used confocal microscopy to demonstrate that both the truncated proteins are expressed and located in the cytoplasm of transfected cells. AIFΔ1-480 but not AIF360-480 induces apoptosis in transfected cells. We also found that the expression of AIFΔ1-480 could initiate the release of cytochrome c from the mitochondria. The suppression of caspase-9 via siRNA blocked the proapoptotic activity of AIFΔ1-480. Therefore, AIFΔ 1-480 is sufficient for inducing caspase-9-dependent apoptotic signaling, probably by promoting the release of cytochrome c. At last, we generated a chimeric immuno-AIFΔ 1-480 protein, which comprised an HER2 antibody, a Pseudomonas exotoxin A translocation domain and AIFΔ 1-480. Human Jurkat cells transfected with the immuno-AIFΔl-480 gene could express and secrete the chimeric protein, which selectively recognize and kill HER2-overexpressing tumor cells. Our study demonstrates the feasibility of the immuno-AIFΔl-480 gene as a novel approach to treating HER2-overexpressing cancers.

Presume-se que a radiação ultravioleta-B (UV-B) e nitrogênio aumentem simultaneamente com as mudanças climáticas globais futuras. Neste estudo, avaliaram-se o crescimento e respostas fotossintéticas de plântulas de Picea asperata ao aumento da radiação ultravioleta-B e ao suprimento de nitrogênio. Utilizaram-se dois tratamentos de UV-B (UV-B ambiente, 11.02 kJ m-2 d-1; e UV-B suplementar, 14.33 kJ m-2 d-1) e duas doses de N (0 e 20 g N m-2), a fim de se determinar se o suprimento de N poderia atenuar os impactos negativos do aumento da radiação UV-B sobre o crescimento e a fotossíntese. O aumento da radiação UV-B inibiu o crescimento e fez reduzir a taxa de fotossíntese líquida, a condutância estomática, a taxa transpiratória, a taxa de fotossíntese saturada à luz, a irradiância de compensação, a taxa de respiração escura, o rendimento quântico aparente, a concentração de pigmentos fotossintéticos e o rendimento quântico potencial das plântulas de P. asperata, enquanto a fluorescência mínima e a concentração intercelular de CO2 aumentaram com o aumento da radiação UV-B. Por outro lado, o suprimento de N afetou positivamente o desempenho fotossintético e o crescimento, porém apenas sob níveis ambientes de radiação UV-B. Com efeito, N não esteve associado à proteção da maquinaria fotossintética das plântulas de P. asperata expostas à radiação UV-B suplementar.

Ultraviolet-B (UV-B) radiation and nitrogen are expected to increase simultaneously with future changes in global climate. In this study, growth and photosynthetic responses of Picea asperata seedlings to enhanced UV-B and to nitrogen supply were studied. The experimental design included two levels of UV-B treatments (ambient UV-B, 11.02 kJ m-2 d-1; enhanced UV-B, 14.33 kJ m-2 d-1) and two nitrogen levels (0; 20 g N m-2) to determine whether nitrogen can alleviate the negative impacts of enhanced UV-B on seedling growth and photosynthesis. Enhanced UV-B significantly inhibited plant growth and impaired net photosynthetic rate, stomatal conductance, transpiration rate, the light-saturated assimilation rate, assimilation capacity, light compensation point, dark respiration rate, apparent quantum yield, photosynthetic pigments and maximum quantum yield of photosynthesis of P. asperata seedlings, whereas minimal fluorescence and intercellular CO2 concentration increased by enhanced UV-B. On the other hand, nitrogen supply improved the photosynthetic performance and plant growth, but only under ambient UV-B. In fact, nitrogen supply could not alleviate the photosynthetic impairments in P. asperata seedlings exposed to enhanced UV-B radiation.