Abstract Objective To explore the feasibility of injectable platelet-rich fibrin (i-PRF) in regenerative endodontics by comparing the effect of i-PRF and platelet-rich fibrin (PRF) on the biological behavior and angiogenesis of human stem cells from the apical papilla (SCAPs). Methodology i-PRF and PRF were obtained from venous blood by two different centrifugation methods, followed by hematoxylin-eosin (HE) staining and scanning electron microscopy (SEM). Enzyme-linked immunosorbent assay (ELISA) was conducted to quantify the growth factors. SCAPs were cultured with different concentrations of i-PRF extract (i-PRFe) and PRF extract (PRFe), and the optimal concentrations were selected using the Cell Counting Kit-8 (CCK-8) assay. The cell proliferation and migration potentials of SCAPs were then observed using the CCK-8 and Transwell assays. Mineralization ability was detected by alizarin red staining (ARS), and angiogenesis ability was detected by tube formation assay. Real-time quantitative polymerase chain reaction (RT-qPCR) was performed to evaluate the expression of genes related to mineralization and angiogenesis. The data were subjected to statistical analysis. Results i-PRF and PRF showed a similar three-dimensional fibrin structure, while i-PRF released a higher concentration of growth factors than PRF ( P <.05). 1/4× i-PRFe and 1/4× PRFe were selected as the optimal concentrations. The cell proliferation rate of the i-PRFe group was higher than that of the PRFe group ( P <.05), while no statistical difference was observed between them in terms of cell mitigation ( P >.05). More importantly, our results showed that i-PRFe had a stronger effect on SCAPs than PRFe in facilitating mineralization and angiogenesis, with the consistent result of RT-qPCR ( P <.05). Conclusion This study revealed that i-PRF released a higher concentration of growth factors and was superior to PRF in promoting proliferation, mineralization and angiogenesis of SCAPs, which indicates that i-PRF could be a promising biological scaffold for application in pulp regeneration. plateletrich platelet rich iPRF i (i-PRF (PRF SCAPs. . (SCAPs) methods hematoxylineosin hematoxylin eosin HE (HE SEM. SEM (SEM) Enzymelinked Enzyme linked ELISA (ELISA iPRFe (i-PRFe PRFe, , (PRFe) Kit8 Kit 8 Kit- CCK8 CCK (CCK-8 CCK- assays ARS, ARS (ARS) Realtime Real time RTqPCR RT qPCR (RT-qPCR analysis threedimensional three dimensional structure <.05. 05 <.05 <.05) 14 1 4 1/4 <.05, >.05. >.05 >.05) importantly regeneration (SCAPs (SEM (PRFe (CCK- (ARS 0 <.0 1/ >.0 (CCK <. >. < >

ABSTRACT Objective: The purpose of this study was to investigate the effect and influencing factors of post-surgical radioactive iodine (RAI) therapy for patients with low- and intermediate-risk differentiated thyroid cancer (DTC). Subjects and methods: A retrospective analysis of 423 low- and intermediate-risk DTC patients admitted to the Department of Nuclear Medicine, Sichuan Provincial People’s Hospital from January 2005 to December 2020 was performed. All patients were treated with surgery, had a postoperative pathological diagnosis, and were treated with RAI, including 89 males and 334 females. Recurrence risk stratification: 143 cases were low-risk, and 280 cases were intermediate-risk. Results: The excellent response (ER) rate for low- and intermediate-risk were 93.7% and 78.2%, respectively (P < 0.05). There were significant differences in age, cumulative dose of [131I], and pretreatment stimulated-Tg (pre-Tg) levels between the low- and intermediate-risk groups (P < 0.05). There were significant differences in the cumulative dose of 131I and pre-Tg levels between ER and the non-ER group (P < 0.05). The area under the curve (AUC) values were 0.799 in the low-risk group, and 0.747 in the intermediate-risk group for the ROC curve by ER status of pre-Tg. The ER rate with RAI treatment decreased with an increase in pre-Tg levels. Conclusion: Pre-Tg was an important factor for RAI treatment decision-making and prognostic evaluation and differed between low-risk and intermediate-risk DTC. Aggressive RAI therapy was recommended for low-risk DTC with pre-Tg ≥ 20.0 ng/mL and in intermediate-risk group with pre-Tg ≥ 10.0 ng/mL. Objective postsurgical post surgical (RAI low intermediaterisk intermediate . (DTC) methods 42 Medicine Peoples People s 200 202 performed surgery diagnosis 8 33 females stratification 14 lowrisk, lowrisk risk, 28 intermediaterisk. risk. Results (ER 937 93 7 93.7 782 78 2 78.2% P 0.05. 005 0.05 0 05 0.05) age 131I, I , [131I] stimulatedTg stimulated Tg preTg pre (pre-Tg nonER non AUC (AUC 0799 799 0.79 0747 747 0.74 preTg. Tg. Conclusion PreTg Pre decisionmaking decision making 20 20. ngmL ng mL 100 10 10. (DTC 4 3 1 9 93. 78.2 00 0.0 [131I 079 79 0.7 074 74 78. 0. 07

Abstract This study investigated the effects of different storage temperatures on the structure and physicochemical properties of starch in frozen nonfermented dough. The results showed that the water activity and water loss rate of dough samples decreased with decreasing storage temperature. The density of hydrogen protons in the dough sample decreased as well. The freezable water content in dough increased with decreasing freezing temperature. The results showed that with decreasing freezing temperature, the formation and recrystallization of ice crystals in the process of freezing storage resulted in an increase in depressions and holes in wheat starch grains, internal and external damage to starch, and a gradual decrease in the relative crystallinity. The porous surface of starch particles and their loose molecular order structure promote the infiltration of water molecules into the interior of the starch particles. The combination of starch chain and water molecules makes the peak viscosity of starch in the dough gradually rise, but diminishes its stability when heated. Compared with the control group, the starch recovery value increased, and the starch enthalpy value increased gradually. The structure and physicochemical properties of starch in nonfermented dough stored at -12 °C and -18 °C were similar.