Resumo Fundamento No início da pandemia de COVID-19, os pacientes com infarto do miocárdio (IM) demoraram para procurar um hospital por medo de contágio ou dificuldades no acesso aos serviços de saúde. Objetivos Avaliar procedimentos de cardiologia intervencionista realizados durante a pandemia de COVID-19 e implicações na abordagem do IM. Métodos Registro prospectivo de 24 centros de hemodinâmica no Brasil, com pacientes adultos submetidos a procedimentos de cardiologia intervencionista entre 26 de maio e 30 de novembro de 2020. Os desfechos foram complicações cardiovasculares (CV) e não CV, morte e IM. A concomitância de COVID-19 foi confirmada com RT-PCR. Técnicas de machine learning foram usadas com modelos não paramétricos de árvores de classificação. Usou-se análise de correspondência simples com o software R. Adotou-se nível de significância de 5%. Resultados Este estudo incluiu 1.282 pacientes, 435 dos quais (33,9%) apresentaram IM: IM com supra de ST (IMCSST), 239 (54,9%); e IM sem supra de ST(IMSSST), 196 (45.1%). Dos 1.282 pacientes, 29 tiveram complicações CV, 47 tiveram complicações não CV e 31 morreram. O diagnóstico de COVID-19 foi confirmado em 77 pacientes (6%), com 15,58% de mortalidade e 6,49% de complicações não CV. A maioria dos pacientes apresentou significativa doença arterial coronariana (63%). Trombo intracoronariano foi mais frequente na presença de IMCSST (3,4%) e COVID-19 (4%). Tempo porta-mesa superior a 12 horas no IMSSST associou-se a 30,8% de complicações, 25% em pacientes com COVID-19. Conclusões Todos os óbitos foram precedidos por complicações CV ou não CV. A presença de COVID-19 foi associada a óbito e complicações não fatais dos pacientes submetidos a procedimentos de cardiologia intervencionista durante a pandemia. COVID19, COVID19 COVID 19, 19 (IM saúde COVID-1 2 Brasil 3 2020 (CV RTPCR. RTPCR RT PCR. PCR RT-PCR classificação Usouse Usou se R Adotouse Adotou 5 5% 1282 1 282 1.28 43 33,9% 339 33 9 (33,9% IMCSST, , (IMCSST) 23 54,9% 549 54 (54,9%) STIMSSST, STIMSSST ST(IMSSST) 45.1%. 451 45.1% . 45 (45.1%) 4 morreram 7 6%, 6 6% (6%) 1558 15 58 15,58 649 49 6,49 63%. 63 63% (63%) 3,4% 34 (3,4% 4%. 4% (4%) portamesa porta mesa associouse associou 308 8 30,8 25 COVID19. 19. COVID1 COVID- 202 128 28 1.2 33,9 (33,9 (IMCSST 54,9 (54,9% ST(IMSSST 45.1 (45.1% (6% 155 15,5 64 6,4 (63% 3,4 (3,4 (4% 30, 20 1. 33, (33, 54, (54,9 45. (45.1 (6 15, 6, (63 3, (3, (4 (33 (54, (45. ( (3 (54 (45 (5

Abstract Background At the beginning of the COVID-19 pandemic, patients with myocardial infarction (MI) took longer to present to hospitals because of fear of contamination and health care access difficulties. Objectives To assess interventional cardiology procedures performed during the COVID-19 pandemic and its implications for MI approach. Methods Prospective registry of 24 cardiac catheterization laboratories in Brazil, with adult patients undergoing interventional cardiology procedures between May 26 and November 30, 2020. The outcomes were cardiovascular (CV) and non-CV complications, death, and MI. Concomitant COVID-19 was confirmed using RT-PCR. Machine learning techniques were used with nonparametric Classification Trees models, and Simple Correspondence Analysis, with R statistical software package. Significance level adopted of 5%. Results This study included 1282 patients, 435 of whom (33.9%) had MI as follows: ST-segment elevation MI (STEMI), 239 (54.9%); and non-ST-segment elevation MI (NSTEMI), 196 (45.1%). Of the 1282 patients, 29 had CV complications, 47 had non-CV complications, and 31 died. The diagnosis of COVID-19 was confirmed in 77 patients (6%), with 15.58% mortality and non-CV complications in 6.49%. Most patients had significant coronary artery disease (63%), and an intracoronary thrombus was more often found in the presence of STEMI (3.4%) and COVID-19 (4%). A door-to-table time longer than 12 hours in NSTEMI was associated with 30.8% of complications, 25% in COVID-19 patients. Conclusions All deaths were preceded by CV or non-CV complications. The presence of COVID-19 was associated with death and non-fatal complications of patients undergoing interventional cardiology procedures during the pandemic. COVID19 COVID 19 COVID-1 (MI difficulties approach 2 Brazil 30 2020 (CV nonCV non RTPCR. RTPCR RT PCR. PCR RT-PCR models Analysis package 5 5% 128 43 33.9% 339 33 9 (33.9% follows STsegment ST segment STEMI, , (STEMI) 23 54.9% 549 54 (54.9%) nonSTsegment NSTEMI, (NSTEMI) 45.1%. 451 45.1% . 45 1 (45.1%) 4 3 died 7 6%, 6 6% (6%) 1558 15 58 15.58 649 49 6.49% 63%, 63 63% (63%) 3.4% 34 (3.4% 4%. 4% (4%) doortotable door table 308 8 30.8 25 nonfatal fatal COVID1 COVID- 202 33.9 (33.9 (STEMI 54.9 (54.9% (NSTEMI 45.1 (45.1% (6% 155 15.5 64 6.49 (63% 3.4 (3.4 (4% 30. 20 33. (33. 54. (54.9 45. (45.1 (6 15. 6.4 (63 3. (3. (4 (33 (54. (45. ( 6. (3 (54 (45 (5

RESUMO: Com o objetivo de desenvolver ferramentas para o manejo simultâneo de Rhynchophorus palmarum e da resinose do coqueiro, avaliou-se o efeito do inseticida tiametoxam e do fungicida ciproconazol sobre larvas de R. palmarum em laboratório. Larvas de ínstares iniciais e intermediários foram alimentadas com dieta artificial contendo 0,1% do inseticida, do fungicida, ou da mistura de ambos. A mortalidade larval foi avaliada diariamente, durante 48 horas, e as médias foram submetidas à análise de variância e comparadas entre si pelo teste de Tukey (p ≤ 0,05). O tiametoxam causou mortalidade de 100% às larvas de ínstares iniciais e de 90% às de intermediários. Em contraste, a mortalidade foi significativamente menor no tratamento ciproconazol (60% para larvas de ínstares iniciais e 0% para as de intermediários) e no controle (0% de mortalidade para ambos). A mistura inseticida/fungicida foi igualmente eficiente (100% para larvas de ínstares iniciais e 86,67% para as de intermediários) ao inseticida sozinho. Diferenças na mortalidade entre as larvas de ínstares iniciais e intermediários foram significativas somente para os tratamentos tiametoxam e ciproconazol. Tais resultados indicam que, nas doses avaliadas, o tiametoxam é tóxico às larvas de ínstares iniciais e intermediários, enquanto o ciproconazol é tóxico somente às de ínstares iniciais. Além disso, o ciproconazol não aumenta a toxicidade do tiametoxam. Larvas de ínstares iniciais são mais sensíveis a tiametoxam e ciproconazol que as de ínstares intermediários. Conclui-se que a mistura tiametoxam + ciproconazol pode ser eficiente no manejo de R. palmarum e de doenças fúngicas associadas a essa praga em coqueiros.

ABSTRACT: With the aim of developing tools for simultaneously managing Rhynchophorus palmarum and the coconut stem bleeding disease, we evaluated the effects of the insecticide thiamethoxam and the fungicide cyproconazole on R. palmarum larvae under laboratory conditions. Early-instar and intermediate-instar larvae were fed on an artificial diet containing 0.1% concentration of insecticide, fungicide or a mixture of both. Larval mortality was assessed daily during the 48 hours after the application of the treatments. Mean mortality values were subjected to analysis of variance and compared by Tukey’s test (p ≤ 0.05). Thiamethoxam caused mortality of 100% of early-instar and 90% of intermediate-instar larvae. In contrast, mortality was significantly lower in the cyproconazole treatment (60% for early-instar and 0% for intermediate-instar larvae) and the control (0% mortality for both treatments). The insecticide/fungicide mixture was equally effective (100% for early-instar and 86.67% for intermediate-instar larvae) to the insecticide only treatment. Differences in mortality between early-instar and intermediate-instar larvae were significant only for the thiamethoxam and cyproconazole treatments. These results indicate that, for the doses used here, thiamethoxam is toxic to both early-instar and intermediate-instar larvae, while cyproconazole is toxic just to early-instar larvae. Moreover, cyproconazole does not increase the toxicity of thiamethoxam. Early-instar larvae are more sensitive to thiamethoxam and cyproconazole than intermediate-instar larvae. We conclude that thiamethoxam + cyproconazole mixture may be effective for managing R. palmarum and associated fungal diseases on coconut palms.

OBJECTIVES: Pituitary-dependent hyperadrenocorticism is the most common cause of naturally occurring hypercortisolism in dogs. CRHR1 expression in human and dog corticotrophinomas suggested that this gene affects pituitary tumorigenesis. The present study aimed to investigate mutations in the CRHR1 coding region in poodles with pituitary-dependent hyperadrenocorticism. METHODS: Fifty poodles with pituitary-dependent hyperadrenocorticism and 50 healthy poodles were studied. Genomic DNA was amplified by PCR and analyzed by Sanger sequencing. RESULTS: The novel CRHR1 p.V97M mutation was identified in one dog. This valine residue, located in the amino-terminal extracellular domain, exhibits high affinity for its corticotropin-releasing hormone (CRH) ligand. Bioinformatic analysis revealed structural rearrangements in the mutant protein, with a 17% increase in the surface binding affinity between CRHR1 and CRH. In vitro functional studies showed that mutant CRHR1 induced higher ACTH secretion than the wild type after stimulation with human CRH. CONCLUSION: These results suggest that germline activating mutations in CRHR1 may be a rare cause of pituitary hyperadrenocorticism in poodles.

This paper aimed to study the mode of inclusion for 7-hydroxyflavone (7HF) in β-cyclodextrin (β-CD) complex in basic (pH 12) and acid (pH 4.5) media. The thermodynamic parameters of the complexation of 7HF with β-CD were evaluated by means of steady-state fluorescence and solubility measurements at several temperatures. The 7HF-β-CD inclusion complex was spontaneously formed at 1:1 stoichiometry in both basic and acid media. The high number of hydrogen bonds formed between the host and the guest molecules favored complexation in the basic medium, with a ground-state equilibrium constant (K) of 990 ± 130 mol-1 L, at 25 ºC. The quantum-mechanics calculations showed that complexation of 7HF (neutral or anionic form) with β-CD occurred mainly via the phenyl group of the flavonoid. This result agreed with the induced circular dichroism signal of 7HF complexed with β-CD.

O objetivo desse trabalho foi caracterizar os defeitos pontuais relacionados aos grupos OH e H2O em quartzos e ametistas, crescidos em cavidades do tipo geodo em basalto da Formação Serra Geral (RS), usando a espectroscopia infravermelha (IV) a -175°C. Em um dos cristais, os espectros foram realizados separadamente nos setores de crescimento r{10<IMG SRC="/img/revistas/rem/v62n3/a050i01.gif" ALIGN="absmiddle">1} e z{01<IMG SRC="/img/revistas/rem/v62n3/a050i01.gif" ALIGN="absmiddle">1}. Na faixa entre 3800 e 3000 cm-1, os espectros apresentaram várias bandas de absorção superpostas a uma intensa banda larga. As bandas atribuídas ao defeito [H4O4]0 foram observadas independentemente da coloração dos cristais, enquanto que as bandas atribuídas ao centro [AlO4/H]0 ocorreram nos quartzos translúcido e levemente amarelado (citrino). Além da intensidade da banda larga, os espectros desses cristais se caracterizaram pela existência da banda a 3595 cm-1. Ao contrário do reportado por outros autores, verificou-se que a concentração de OH e H2O e a coloração violeta da ametista são maiores nos setores z do que nos setores r. Essa inversão foi discutida em função da não uniformidade das condições de crescimento, confirmada pelo tamanho das faces romboédricas do cristal. Concluiu-se que a intensidade da coloração da ametista não é afetada pelas altas concentrações de defeitos OH e H2O.

The aim of this study is to characterize OH-related defects in natural quartz and amethyst grown in geodic cavities formed during basaltic flow. For this, infrared (IR) spectroscopy at low temperature (-175°C) was carried out in amethyst and quartz crystals taken from two deposits located in the State of Rio Grande do Sul in Brazil. For one specimen, IR spectra were recorded directly upon the growth sectors r{10<IMG SRC="/img/revistas/rem/v62n3/a050i01.gif" ALIGN="absmiddle">1} and z{01<IMG SRC="/img/revistas/rem/v62n3/a050i01.gif" ALIGN="absmiddle">1} appearing in (0001) plates. IR spectra recorded from 3800 to 3000 cm-1 showed an intense broad band superposed upon sharp bands assigned to [H4O4]0 point defects. These bands were observed in all samples and growth sectors independently from the intensity of its violet color. On the other hand, sharp bands assigned to [AlO4/H]0 defects were observed only in translucent and pale citrine quartz and also in the colorless regions of r and z growth sectors in amethyst. Contrarily to previously reported data, it was observed that z growth sectors have the highest content of OH-related defects and the darkest violet color when compared to r sectors. This reversal relation was discussed taking into account the malformed habitus of the amethyst specimen probably caused by the anisotropic solution flow inside the geode. It was concluded that the amethyst color is independent of the concentration of OH-related defects. Besides the intense broad OH-band, the IR spectra of such crystals were characterized by the presence of a sharp band near 3595 cm-1.

In this work we briefly review the wave packet approach of two generation neutrino oscillation, aiming its description in a progenitor star supernova environment. We begin calculating the wave packet size in many situations: solar and supernova plasma medium, considering nuclear interactions, as well as accelerators and reactors. This quantity is important to calculate the coherence length, which we compare with the oscillation length to verify if neutrino oscillation will or will not occur. Finally, we compare the wave packet treatment of neutrino oscillation with the plane wave formalism in a progenitor star supernova environment.

Foi realizado um estudo epidemiológico em 65 granjas de suínos situadas na região Sul do Brasil para identificar os fatores que favorecem a ocorrência do vício de sucção entre leitões desmamados. Em cada granja acompanhou-se um lote de leitões do nascimento até 21 dias após o desmame, provenientes de no mínimo seis leitegadas, para obtenção de informações relacionadas às instalações, à nutrição, às práticas de manejo, ao ambiente interno e ao desempenho dos leitões. Os dados foram submetidos à análises descritivas e de correspondência múltipla. A manifestação do vício ocorreu em 23 (35,4%) granjas. Nos lotes que apresentaram o vício de sucção, os leitões obtiveram média de ganho diário de peso (P<0,005) inferior aos que não apresentaram o vício de sucção. Os principais fatores de risco identificados foram: peso médio ao desmame menor que 6,3kg, ausência de bebedouro específico para os leitões na maternidade, ocorrência de diarréia na primeira semana após o desmame, tipo de bebedouro usado na creche diferente daquele usado na maternidade, orientação do eixo do prédio inadequado, presença de sinais de sarna no lote, ausência de vazio sanitário na creche e uso da restrição alimentar logo após o desmame. Sugere-se que a correção dos fatores de risco previna a manifestação do vício de sucção e melhore a média de ganho diário de peso dos leitões após o desmame.

Risk factors associated with the suckling vice in post-weaning piglets were studied in an epidemiological survey in 65 swine herds in South Brazil. This follow-up study was performed in one group of piglets from each herd from birth to 21 days post weaning. Every group was composed by piglets from at least six litters. Data related to housing, feeding, breeding practices, internal environment in the weaning facilities and individual piglets performance were collected for descriptive and multiple correspondence analyses. Suckling vice was identified in 23 (35.4%) herds and groups presenting suckling vice had lower growth rates (P<0.005). Main risk factors associated with suckling vice were weaning weight lower than 6.3kg, unavailability of drinkers for piglets in the farrowing house, occurrence of post-weaning diarrhea, post-weaning drinkers of a different model than the ones available during the suckling term, inadequate axis direction of the building, presence of signs of mange, no all-in-all-out procedure in the post-weaning facilities, and the use of feed restriction immediately after weaning. The authors suggest that a correction of these factors would reduce the occurrence of suckling vice in post-weaning piglets and improve their grow rates after weaning.

Realizou-se um estudo em 64 granjas de suínos na região sul do Brasil entre julho de 1995 e março de 1997 com o objetivo de estimar o índice de pneumonia (IP) e o índice de rinite atrófica (IRA), por meio da contagem dos sinais clínicos de tosse e espirro, respectivamente, em suínos em crescimento-terminação. Em cada granja, um lote com cerca de 60 suínos foi acompanhado desde o alojamento na fase de crescimento até o abate. Tosse e espirro foram contados em quatro oportunidades (30, 50 e 80 dias após o alojamento e 1 a 3 dias antes do abate). Em cada oportunidade foram feitas três contagens de dois minutos cada e o percentual de tosse e espirro para cada lote foi calculado pela média das três contagens em relação ao tamanho do lote. No abate, os suínos foram avaliados quanto à freqüência e severidade de lesões de hepatização pulmonar e de rinite atrófica nos cornetos nasais para cálculo do IP e do IRA, respectivamente. Os dados foram submetidos às análises de correlação de Pearson e de regressão simples para ajuste da equação de predição do IP em relação à porcentagem de tosse e do IRA em relação à porcentagem de espirro. As equações obtidas foram: IPe = 0,35 + (0,11 × % de tosse) com R² = 0,45 e IRAe = 0,36 +( 0,065 × % de espirro) com R² = 0,36. Conclui-se que é possível utilizar o método de contagem de tosse e espirro na fase de crescimento-terminação para estimar, respectivamente, o IP e o IRA.

A study in 64 swine herds was carried out in order to estimate the pneumonia index (PI) and the atrophic rhinitis index (ARI) through the quantification of coughing and sneezing in growing-finishing pigs. In each herd, 60 pigs were evaluated from lodging in the growing-finishing phase up to slaughter. The amount of coughing and sneezing episodes was counted in four opportunities (30, 50 and 80 days after lodging, and three days before slaughter). In each opportunity three counts of two minutes each were made and the percentage of coughing and sneezing was calculated by the average of the three counts in relation to the batch size. At slaughter, for all pig-batches the frequency and severity of lung consolidation and atrophic rinithis were determined and scored. All data were submitted to the Pearson correlation and to the simple regression analyses of the PIe in relationship to the percentage of coughing and ARIe in relationship to the percentage of sneezing. The resulting equation were: PI estimated = 0.35 + (0.11 × % of coughing) with R² = 0.45, and ARI estimated = 0.36 + (0.065 × % of sneezing) with R² = 0.36. It is concluded that the quantification of coughing and sneezing can be used to estimate ARI and PI indexes in growing-finishing pigs.