Pesquisa | SciELO

ABSTRACT Species of the Amaranthus genus are very versatile and have potential for the application in the development of commercial products. The near infrared spectroscopy (NIR) is an efficient tool that can help in the quality control of products, quickly and non-destructive to the sample. The goal of this study was to carry out the distinction of seed and oils of different Amaranthus species using the near infrared spectroscopy. Three species were used: A. viridis L., A. hybridus L. e Amaranthus sp. (commercial). The spectra acquired from the sample using the near infrared spectroscopy were submitted to the partial least squares discriminant analysis (PLS-DA) and to the principal component analysis (PCA). Through PCA, it was possible to differentiate the Amaranthus species both for seeds and oils. Through PLS-DA, it was possible to predict the classes of the species with high degree of correct classification, with 96.67% of correct classifications for seeds and 98.89% for oil. Thus, with the use of the near infrared spectroscopy associated with the multivariate statistical analysis, it is possible to classify the different Amaranthus species, especially when using the oil.

https://doi.org/10.1590/0034-737x202370030004

Agrobacterium rhizogenes-Mediated Transformation of Crotalaria ochroleuca: Production of Flavonoids from Hairy Roots
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Blank, Daiane E.

; Demuner, Antonio J.

; Carvalho, Jilma L. B.

; Firmino, Maria J. M.

; Figueiredo, Tainá S.

; Souza, Gustavo S. F.

; Zocolo, Guilherme J.

; Guedes, Jhonyson A. C.

; Faria, Daniele V.

; Vieira, Lorena M.

; Soares, Jéssica R.

; Fortini, Evandro A.

; Santos, Marcia A. C.

; Otoni, Wagner C.

Journal of the Brazilian Chemical Society

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2023, Volume 34 Nº 12 Páginas 1898 - 1908

Resumo: En | Texto: En | PDF: En

Crotalaria ochroleuca (Fabaceae) is rich in bioactive compounds used for nematode control. Transformed root culture allows the production of a large amount of hairy root biomass, conditioned by auxin biosynthesis-related genes integrated via the process of Agrobacterium rhizogenes infection, which is known to cause a hairy root phenotype. We transformed A. rhizogenes to increase the biomass of hairy roots and optimized the process of production of bioactive compounds. The transformed nature of hairy roots was confirmed by polymerase chain reaction, which confers kanamycin resistance. The transformed roots were cultured in full-strength in liquid media to realize the growth of hairy roots and the production of bioactive compounds. The transformed roots were grown in a culture medium supplemented with elicitors to produce total phenols, and flavonoids. Chromatographic analysis of transformed roots revealed the presence of flavonoids apigenin-6,8-C-diglucoside and luteolin-6-C-glucoside. The results were obtained by conducting physiological and biochemical studies with the Flavonoids and studying the pathways that led to the production of large amounts of bioactive compounds from the hairy roots of C. ochroleuca. It was observed that the extraction of the compounds significantly affected nematodes and insect larvae, resulting in significantly high levels of economic damage to crops.

https://doi.org/10.21577/0103-5053.20230083

Mortality of large macaws by electrocution in an urban area, Campo Grande, Mato Grosso do Sul, Brazil
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Tinoco, Larissa

; Calderan, Aline

; Appel, Sabrina Cristiane

; Vicente, Eliane

; Guedes, Neiva Maria Robaldo

Ambiente & Sociedade

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2022, Volume 25 elocation e00182

Resumo: Pt En Es | Texto: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Resumo A eletrocussão é tema pouco relatado para as grandes araras. O objetivo deste trabalho foi analisar a mortalidade das araras por eletrocussão na área urbana de Campo Grande, Mato Grosso do Sul. Entre 2011 e 2020 foram registradas 59 araras mortas por eletrocussão, tendo como vítimas duas espécies: Ara ararauna (Linnaeus, 1758) e a Ara chloropterus (Gray, 1859). A. ararauna foi a espécie com maior incidência, com 48 aves mortas, seguida da A. chloropterus, com 11 indivíduos. A eletrocussão foi um importante fator de perda das grandes araras no ecossistema urbano. O tamanho corporal, a envergadura das asas e o comportamento das araras, bem como a localização e proximidade das estruturas elétricas com os recursos alimentares e de nidificação favoreceram as chances de eletrocussão. Logo, é necessário desenvolver medidas de mitigação para conciliar o desenvolvimento sustentável de uma cidade de porte médio como Campo Grande com a conservação da biodiversidade.

Abstract Electrocution is a theme that has been rarely reported for large macaws. The objective of this study was to analyze mortality of macaws due to electrocution in an urban area of Campo Grande, Mato Grosso do Sul. Between 2011 and 2020, 59 macaws were registered as having died through electrocution, and these were from two species: Ara ararauna (Linnaeus, 1758) and Ara chloropterus (Gray, 1859). A. ararauna had the largest number of deaths registered (48), and A. chloropterus had 11 individuals. Electrocution was shown to be an important factor for the loss of large macaws in this urban environment. Body size, wingspan, and behavior of the macaws, as well as location and proximity of food resources and nests to electrical structures, were shown to increase the probability of electrocution. These results demonstrate the need to develop mitigation measures to reconcile the sustainable development of a medium-sized city such as Campo Grande with biodiversity conservation.

Resumen La electrocución es tema poco reportada en los guacamayos. El objetivo de este trabajo fue analizar la mortalidad de guacamayos en el área urbana de Campo Grande, Mato Grosso do Sul. Entre 2011 y 2020 fueron registrados 598 guacamayos muertos por electrocución teniendo como víctimas, dos espécies: Ara ararauna (Linnaeus, 1758) y Ara chloropterus (Gray, 1859). A. ararauna fue la especie con más incidencia, con 48 aves muertas, seguida de A. chloropterus , con 11 individuos. La electrocución es la causa importante de pérdida para los guacamayos en el ecosistema urbano. El tamaño corporal, la envergadura de las alas y el comportamiento urbano de los guacamayos, bien como la localización y la proximidad de los tendidos eléctricos con los recursos alimenticios y de nidificación favorecido las posibilidades de electrocución. Por lo tanto, es necesario desarrollar medidas de mitigación conciliar el desarrollo sustentable de una ciudad mediana como Campo Grande com la conservación de la biodiversidad.

https://doi.org/10.1590/1809-4422asoc20200018r2vu2022l1ao

Atención extrahospitalaria en una Unidad Febril de Urgencia de pacientes sospechosos de Covid 19
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Aprea, Valeria

; Debaisi, Gustavo E.

; Guedes, Verónica

; Guglielmo, María C.

; Miño, Laura

; Stabilito, Luis A.

; Yazde Puleio, María Liliana

Andes pediatrica

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Out 2021, Volume 92 Nº 5 Páginas 677 - 682

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract: Introduction: In 2019, a new infection was reported in China. This coronavirus was named SARS-COV-2, causative of the 21st-century pandemic, COVID-19. Health systems adopted different strategies to cope with it. Objective: to describe the clinical-epidemiological characteristics of COVID-19 in children seen at an Emergency Febril Unit (UFU). Patients and Method: Cross-sectional study in patients under 18 years of age tested for SARS-COV-2 between April 1 and June 30, 2020. All epidemiological re cords made at the time of consultation and the result of the Polymerase Chain Reaction (PCR) test of these patients, either by suspicion of COVID-19 or epidemiological isolation criteria, were inclu ded. Patients whose samples had been taken for SARS-COV-2 determination outside the initial time of consultation or whose epidemiological records were incomplete or did not meet the established inclusion criteria were excluded. The diagnosis of COVID-19 was made using the PCR technique for SARS-COV-2 in nasopharyngeal secretions obtained by nasopharyngeal swab or aspirate. The following variables were recorded: age, gender, place of residence, history of close contact, history of history of close contacts, travel history and comorbidities, history of institutionalization and PCR result. Results: 1,104 patients were admitted to the UFU and tested due to suspected COVID-19. 152 patients had to be excluded due to insufficient data. Of the 952 patients tested, 22.6% had a detectable result, and 71.2% of them reported close contact with confirmed cases. The mean age was 5.9 years. The 55.4% were male and 99.3% lived in the Metropolitan Area of Buenos Aires. 72.8% of the patients tested had symptoms. The time of delay in consultation was 2.17 days. 25% of the children had comorbidities. Conclusions: The availability of the UFU facilitated access and optimized the care circuit in response to demand. Children with a history of close contact and those symptomatic showed more frequently a detectable result for SARS-COV-2.

Resumen: Introducción: En 2019 una nueva infección fue informada en China. Este coronavirus se denominó SARS-COV-2, causal de la pandemia del siglo XXI, COVID-19. Los sistemas de salud asumieron diferentes estrate gias para enfrentarlo. Objetivo: describir las características clínico-epidemiológicas del COVID-19 en niños atendidos en una Unidad Febril de Urgencia (UFU). Pacientes y Método: Estudio transversal en pacientes menores de 18 años testeados para SARS-COV-2 entre el 1 de abril y el 30 de junio de 2020. Se incluyeron todas las fichas epidemiológicas confeccionadas al momento de la consulta y el resultado de la prueba de Reacción en Cadena de la Polimerasa (PCR) de dichos pacientes, ya sea por sospecha de COVID-19 o por criterios de aislamiento epidemiológico. Se excluyeron los pacientes a quienes se les hubiera tomado muestra para determinación de SARS-COV-2 fuera del momento inicial de consulta o cuyas fichas epidemiológicas estuvieran incompletas o no cumplieran con los criterios de inclusión establecidos. El diagnóstico de COVID-19 se realizó mediante la técnica de PCR para SARS-COV-2 en secreciones nasofaríngeas obtenidas por hisopado o aspirado nasofaríngeo. Se registraron las siguientes variables: edad, genero, lugar de residencia, antecedentes de contactos estrechos, antecedentes de viaje, comorbilidades, antecedentes de institucionalización y resultado de PCR. Resultados: En total, 1104 pacientes fueron ingresados en la UFU y testeados por sospecha de COVID-19. Debieron ser excluidos 152 pacientes por datos insuficientes. De los 952 analizados el 22,6% presentó un resultado detectable, y de ellos un 71,2% refirió contacto estrecho con casos confirmados. La edad media fue de 5,9 años. El 55,4% fue de sexo masculino y 99,3% vivían en el Área Metropolitana de Buenos Aires. Un 72,8% de los pacientes testeados presentaban síntomas. El tiempo de demora en la consulta fue de 2,17 días. El 25 % de los niños tenían comorbilidades. Con clusiones: La disponibilidad de la UFU facilitó el acceso y optimizó el circuito de atención frente a la demanda. Los niños con antecedentes de contacto estrecho y aquellos sintomáticos mostraron con más frecuencia un resultado detectable para SARS-COV-2.

https://doi.org/10.32641/andespediatr.v92i5.3422 26 downloads

Spondias purpurea L. Stem Bark Extract: Antioxidant and in vitro Photoprotective Activities
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Rodrigues, Francisco Alessandro M.

; Giffony, Priscylla S.

; Santos, Sarah B. F. dos

; Guedes, Jhonyson A. C.

; Ribeiro, Maria Elenir N. P.

; Araújo, Tamara G. de

; Silva, Larissa M. R. da

; Zocolo, Guilherme J.

; Ricardo, Nágila M. P. S.

Journal of the Brazilian Chemical Society

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Out 2021, Volume 32 Nº 10 Páginas 1918 - 1930

Resumo: En | Texto: En | PDF: En

Plant phenolic extracts are widely recognized as an important source of natural antioxidant substances and potential compounds for cosmetic formulations. This study aimed to evaluate the chemical profile, photoprotective and antioxidant activities of stem bark extract of Spondias purpurea L. (ciriguela) and its application in photoprotective formulations. Thirty phenolic constituents were annotated by ultra-performance liquid chromatography coupled with an electrospray ionization quadrupole time-of-flight mass spectrometry in mode negative (UPLC-QTOF-MS2). The stem bark extract antioxidant and chelation potential, expressed in half maximal inhibitory concentration (IC50), showed 6.25 and 352.22 µg mL−1, respectively. The phenolic extract was used as an active ingredient in six sunscreen formulations, with concentrations ranging from 0.2 to 10%. The ultraviolet (UV) protection properties of the formulations were evaluated by sun protection factor (SPF) values obtained in 0.2 mg mL−1 (0.495 to 2.27) and 2.0 mg mL−1 (2.29 to 15.87). The SPF value for the extract (14.37 and 26.16) was high, but there was a reduction in the base formulation. However, these results suggested that the bioactive extracted of stem bark of Spondias purpurea L. has interesting potential to reduce the damage caused by UV radiation and may be utilized as an active ingredient in a sunscreen formulation.

https://doi.org/10.21577/0103-5053.20210082 46 downloads

Plasma energetic substrates and hepatic enzymes in the four-eyed fish Anableps anableps (Teleostei: Cyprinodontiformes) during the dry and rainy seasons in the Amazonian Island of Maracá, extreme north of Brazil
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Guedes, Maria Eduarda Gomes

; Correia, Tiago Gabriel

Neotropical Ichthyology

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2021, Volume 19 Nº 4 elocation e210078

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Anableps anableps é um teleósteo vivíparo típico de estuários do Delta do Rio Amazonas, sendo representativo deste bioma na ilha de Maracá, com potencial para o biomonitoramento. O objetivo deste estudo foi aplicar diferentes biomarcadores em machos e fêmeas desta espécie e verificar a possível influência sazonal em aspectos de sua fisiologia. Para a coleta dos peixes foram realizadas três expedições, de abril/2018 (estação chuvosa), setembro/2018 (estação seca) até fevereiro/2019 (estação chuvosa). Foram obtidos parâmetros biométricos, índices gonadossomático (IGS), hepatossomático (IHS), viscerossomático (IVS) e amostras de sangue para dosagem de triglicerídeos, proteínas totais, glicose e a atividade das enzimas aspartato aminotransferase (AST), alanina aminotransferase (ALT) e fosfatase alcalina (ALP). O IGS dos machos foi maior na estação chuvosa e das fêmeas na estação seca, isso provavelmente ocorreu devido ao processo de embriogênese. Os machos aumentaram a biomassa na estação seca e apresentaram homogeneidade metabólica, já as fêmeas apresentaram hiperglicemia, hipertrigliceridemia e maior atividade da ALT. Os biomarcadores testados mostraram-se promissores para o biomonitoramento, sugerindo de forma preliminar que há um assincronismo sazonal entre machos e fêmeas de A. anableps na alocação de recursos energéticos em diferentes momentos do seu ciclo de vida.

ABSTRACT Anableps anableps is a viviparous teleost typical from Amazon Delta estuaries. It is representative of this biome in Maracá, which offers a potential for biomonitoring. The aim of this study is to apply different biomarkers to males and females of this species and verify possible seasonal influences on their physiology. To collect fish, three expeditions were carried out from the rainy season of April 2018 to the rainy season of February 2019. Biometric parameters and gonadosomatic (GSI), hepatosomatic (HSI), and viscerosomatic (VSI) indexes were calculated, and blood samples were taken to measure triglycerides, total proteins, glucose, and activity of the enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). The GSI of males is higher in the rainy season and of females in the dry season. This is probably related to the embryogenesis process. Males show an increase in biomass during the dry season, a metabolic homogeneity, and females show an increase in plasma glucose, triglycerides, and ALT activity. The tested biomarkers are potential for biomonitoring, preliminarily suggesting that there is a seasonal asynchronism between males and females of A. anableps as for the allocation of energy resources at different times of their life cycle.

https://doi.org/10.1590/1982-0224-2021-0078

Metabolomics-Based Discovery of Biomarkers with Cytotoxic Potential in Extracts of Myracrodruon urundeuva
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Castro, Caio B.

; Luz, Licia R.

; Guedes, Jhonyson A. C.

; Porto, Diogo D.

; Silva, Maria Francilene S.

; Silva, Gisele S.

; Ribeiro, Paulo R. V.

; Canuto, Kirley M.

; Brito, Edy S.

; Zampieri, Dávila S.

; Pessoa, Claúdia Ó.

; Zocolo, Guilherme J.

Journal of the Brazilian Chemical Society

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Abr 2020, Volume 31 Nº 4 Páginas 775 - 787

Resumo: En | Texto: En | PDF: En

Myracrodruon urundeuva (“aroeira-do-sertão”) is a species threatened with extinction due to anthropogenic exploitation. Phytochemical analysis of bark, branch and leaf extracts revealed the presence of several compounds such as flavonoids, phenols, tannins, quercetin derivatives and anacardic acids. Dereplication methodology was performed to tentatively identify 50 compounds analyzed by ultra-performance liquid chromatography coupled with an electrospray ionization quadrupole time-of-flight mass spectrometry operating in MSE mode (UPLC-QTOF-MSE). The extracts exhibited anti-tumor effect in cancer cells HCT-116 (colorectal), SF-295 (glioblastoma), HL-60 (leukemia), and RAJI (leukemia). Also, these results correlate with the principal component analysis (PCA) data that identified three distinct groups indicating, efficiently, metabolic differences between organs of M. urundeuva. Through discriminatory analysis of the orthogonal partial least squares (OPLS-DA), the variable of importance in the projection (VIP) and S-Plot, we were able to determine 30 potential biomarkers. The fingerprint of hydroethanolic extracts was correlated with the cytotoxicity assay and demonstrated a significant difference in the composition of plant extract.

https://doi.org/10.21577/0103-5053.20190242 361 downloads

GC-MS-Based Metabolomic Profiles Combined with Chemometric Tools and Cytotoxic Activities of Non-Polar Leaf Extracts of Spondias mombin L. and Spondias tuberosa Arr. Cam.
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Guedes, Jhonyson A. C.

; Alves Filho, Elenilson G.

; Silva, Maria F. S.

; Rodrigues, Tigressa H. S.

; Ramires, Christiane M. C.

; Lima, Maria A. C.

; Silva, Gisele S.

; Pessoa, Cláudia Ó.

; Canuto, Kirley M.

; Brito, Edy S.

; Alves, Ricardo E.

; Nascimento, Ronaldo F.

; Zocolo, Guilherme J.

Journal of the Brazilian Chemical Society

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Fev 2020, Volume 31 Nº 2 Páginas 331 - 340

Resumo: En | Texto: En | PDF: En

Agroindustrial residues, such as leaves of fruit plants, can be sources of bioactive molecules, thus adding value to co-products that are rarely explored in agroindustry. In this context, this study aimed to look into the phytochemical profiles in detail and to explore the antitumor potential of S. tuberosa and S. mombin leaves. We observed that, S. tuberosa leaf extract was cytotoxic in both tumor and healthy cells. S. mombin extracts selectively inhibited cell proliferation in the tumor cell line for prostate cancer (PC3) and did not significantly affect healthy cells. The metabolic profiles of the extracts were evaluated by gas chromatography coupled with mass spectrometry and twenty-three metabolites were identified. The correlation of metabolic profiles with cytotoxic tests indicated possible chemical markers that may be responsible for the inhibition of cell proliferation. This study revealed that the unexplored co-products in agroindustry may have great therapeutic potential, and therefore should be screened for biologically active compounds.

https://doi.org/10.21577/0103-5053.20190185 596 downloads

Shading Levels and Substrates Affect Morphophysiological Responses and Quality of Anadenanthera peregrina (L.) Speg Seedlings
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Santos, Cleberton Correia

; Jorge, Higor Périkles Guedes

; Dias, Luiz Gabriel Fernandes

; Vieira, Maria do Carmo

Floresta e Ambiente

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2020, Volume 27 Nº 2 elocation e20190119

Resumo: En | Texto: En | PDF: En

Abstract It is essential to know the ecological tolerance of Anadenanthera peregrina (L.) Speg seedlings regarding the light and edaphic conditions. The aim of this study was to evaluate the effect of substrates and shading levels on the emergence and quality of A. peregrina seedlings. The seeding was done in two substrates: 100% Dystroferric Red Latosol (DRL) and DRL + commercial substrate (1:1, v/v). The species were kept under four shading levels: 0% (direct sunlight), 30%, 50% and 70%. The highest speed and shortest emergence time of A. peregrina occurred in DRL. The highest growth characteristics of aerial part, xylopodium and roots occurred under direct sunlight and DRL. The productions of fresh and dried masses of different vegetative organs, physiological and Dickson quality index were higher in DRL and direct sunlight. The A. peregrina seedlings in 100% of DRL and direct sunlight showed more viable morphophysiological and quality indicators for their production.

https://doi.org/10.1590/2179-8087.011919 531 downloads

10.

Bioactividad de Spilanthes acmella (Asteraceae) a Raoiella indica (Acari: Tenuipalpidae) y selectividad al depredador Amblyseius largoensis (Acari: Phytoseiidae)
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Rabelo Coelho, Caroline

; Clezia dos Santos, Maria

; Viteri-Jumbo, Luis

; Guedes de Sena Filho, José

; Neoob de Carvalho Castro, Karina

; Marques Canuto, Kirley

; Sousa de Brito, Edy

; Sheila de Queiroz Souza, Ana

; Vieira Teodoro, Adenir

Revista de Protección Vegetal

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Abr 2019, Volume 34 Nº 1 elocation e08

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

ABSTRACT The jambu, Spilanthes acmella (Asteraceae), has bioactivity on pests, but little is known of the effect on phytophagous mites and its compatibility with predatory mites. The red palm mite, Raoiella indica, is a severe pest of several crops, coconut trees among them. The predatory mite Amblyseius largoensis (Acari:Phytoseiidae) is found in association with R. indica in several countries, and it can assist in the control of this pest in the field. Associated with biological control, products that are compatible with natural enemies could help control this pest. Therefore, the acaricidal activity of S. amella on R. indica and its selectivity for the predator A. largoensis was evaluated using concentration-mortality bioassays of its ethanolic extract (EE). Additionally, the repellent effect and the population growth rate were evaluated in the LC50 and LC80. The EE of S. acmella was highly toxic to R. indica, but it did not kill A. largoensis. The LC50 and LC80 of the EE from S. acmella, estimated for R. indica, were repellent to the pest untill 48 hours after application, while, at its highest concentration, it repelled.

RESUMEN El jambu, Spilanthes acmella (Asteraceae), posee bioactividad contra plagas; sin embargo, es poco conocido sobre el efecto a ácaros fitófagos y su compatibilidad con ácaros depredadores. El ácaro rojo del cocotero Raoiella indica es una plaga severa de diversos cultivos agrícolas, dentro de los cuales está el cocotero. El ácaro depredador Amblyseius largoensis (Acari: Phytoseiidae) se encuentra en asociación con R. indica en diversos países y puede auxiliar en el control de esta plaga en campo. Asociado al control biológico, los productos que sean compatibles con los enemigos naturales pueden ayudar en el control de esta plaga. Se evaluó la actividad acaricida por medio de bioensayos de concentración-mortalidad del extracto etanólico (EE) de S. acmella contra R. indica y su selectividad al depredador A. largoensis. Además, se evaluaron el efecto repelente y la tasa de crecimiento poblacional en la CL50 y la CL80. La CL50 y la CL80 de EE de S. acmella, estimada para R. indica, fueron repelentes a la plaga hasta 48 horas después de la aplicación y repelió al depredador solamente hasta una hora en la mayor concentración. Esas concentraciones del EE de S. acmella redujeron drásticamente la tasa de crecimiento de R. indica y no afectaron el aumento poblacional del depredador. Se concluye que el EE de S. acmella podría ser utilizado en el manejo de R. indica por ser tóxico, repelente y reducir el crecimiento poblacional de esta plaga, además de ser selectivo al ácaro depredador A. largoensis.

11.

Crescimento de algodoeiro colorido cv. BRS Topázio em solos com distintas salinidades e adubação orgânica
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Barbosa, Joicy L.

; Nobre, Reginaldo G.

; Souza, Leandro de P.

; Veloso, Luana Lucas de S. A.

; Silva, Elcimar Lopes da

; Guedes, Maria Amanda

Revista de Ciências Agrárias

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Mar 2019, Volume 42 Nº 1 Páginas 201 - 210

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

O algodão de fibra naturalmente colorida vem despertando interesse dos produtores e consumidores no Brasil e no mundo, tendo em vista ser um mercado crescente, com grande potencial socioeconômico para fixação de mão de obra, geração de empregos e fonte de matéria-prima para a indústria têxtil. Diante do exposto, objetivou-se avaliar o crescimento do algodoeiro cv. BRS Topázio cultivado em solos de distintas sodicidades e adubação orgânica em condições de ambiente protegido no CCTA/UFCG. Usou-se o delineamento experimental de blocos ao acaso, em esquema fatorial 5 x 4, com três repetições, sendo os tratamentos compostos de cinco percentagens de sódio trocável (PST) (8,84; 12,55; 18,80; 28,80 e 38,80%) associado ao fator níveis de esterco caprino, sendo 0; 5; 10 e 15% em base de volume do vaso. A PST a partir de 24% afeta a área foliar e a produção de matéria fresca tem sua produção reduzida a partir da PST de 38,14% e seca de folha tem sua produção reduzida a partir da PST de 32,60%. A adubação com esterco caprino até 15% promove incremento nas variáveis de crescimento e produção de fitomassa do algodoeiro cv. BRS Topázio. Não houve interação entre os fatores percentagem de sódio trocável e doses de esterco caprino sobre as variáveis em estudo.

Naturally colored fiber cotton has been attracting interest from producers and consumers in Brazil and around the world, with a view to being a growing market with great socioeconomic potential for labor, employment and a source of raw material for industry textile. Regarding the above, the objective was to evaluate the growth and phytomass production of cotton cv. BRS Topaz cultivated in soils of different PSTS and doses of bovine manure under protected environment conditions in CCTA/UFCG. The experimental design was a randomized complete block design in a 5 x 4 factorial scheme with three replicates, and the treatments were composed of five percent exchangeable sodium (PST) (8.84, 12.55, 18.80, 28.80 and 38.80%) associated to the goat manure factor, being 0, 5, 10 and 15% of the volume of the pot. The PST from 24% affects the leaf area and the fresh and dry leaf has its production reduced from the PST of 38.14 and 32.60% respectively.Fertilization with bovine manure promotes an increase in growth and phytomass production of cotton cv. BRS Topaz. There was no interaction between the percentage of exchangeable sodium and doses of bovine manure on the variables under study.

https://doi.org/10.19084/RCA17294 511 downloads

12.

Superimposing a high-fat diet on Schistosoma mansoni infection affects renin-angiotensin system components in the mouse kidney
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Lira, Danielle Guedes Dantas

; Oliveira, Danielly Cantarelli de

; Brayner, Fábio André

; Aires, André de Lima

; Albuquerque, Mônica Camelo Pessoa A.

; Vieira, Leucio Duarte

; Castro, Célia Maria Machado Barbosa de

; Paixão, Ana Durce

Revista da Sociedade Brasileira de Medicina Tropical

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2019, Volume 52 elocation e20180371

Resumo: En | Texto: En | PDF: En

Abstract INTRODUCTION: The levels of the full-length form of the (pro)renin receptor (PRR), a component of the renin-angiotensin system (RAS), may be reduced in the membranes of kidneys in renal diseases. This study aimed to investigate the RAS components in the kidneys of mice submitted to a combination of a high-fat diet and Schistosoma mansoni infection. METHODS: Female BALB/c mice were maintained on a control or high-fat diet from 3 weeks of age. After 10 weeks on the designated diets, half the mice in each group were infected with S. mansoni cercariae. The blood and kidneys were harvested 8 weeks after infection. RESULTS: The high-fat diet increased the number of eggs in the feces and the number of adult worms in the mesenteric bed. Schistosoma mansoni infection reduced the plasma levels of glucose, triglycerides, and HDL cholesterol in the control and high-fat diet groups. In mice on the control diet, S. mansoni infection resulted in increased expression of IL-6 in the kidneys; however, in mice on the high-fat diet, the levels of IL-6 were reduced and those of superoxide anions were increased. The RAS components evaluated were ACE2, renin, PRR, AT1R, and AT2R, and the levels of PRR were found to be reduced in the kidneys of infected mice on the high-fat diet. CONCLUSIONS: The finding regarding PRR is not yet clear. However, combining a high-fat diet and S. mansoni infection resulted in increased oxidative stress in the kidney that can aggravate hypertension as well as its associated complications.

https://doi.org/10.1590/0037-8682-0371-2018 1639 downloads

13.

Incidence of tuberculosis among patients with rheumatoid arthritis using TNF blockers in Brazil: data from the Brazilian Registry of Biological Therapies in Rheumatic Diseases (Registro Brasileiro de Monitoração de Terapias Biológicas - BiobadaBrasil)
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Yonekura, Claudia Leiko

; Oliveira, Rene Donizeti Ribeiro

; Titton, David C.

; Ranza, Roberto

; Ranzolin, Aline

; Hayata, André L.

; Duarte, Ângela

; Silveira, Inês G.

; Carvalho, Hellen M. da S. de

; Moraes, Júlio C. Bertacini de

; Abreu, Mirhelen Mendes de

; Valim, Valéria

; Bianchi, Washington

; Brenol, Claiton Viegas

; Pereira, Ivanio A.

; Costa, Izaias

; Macieira, José C.

; Miranda, José R.S.

; Guedes-Barbosa, Luiz S.

; Bertolo, Manoel B.

; Sauma, Maria Fátima Lobato da C.

; Silva, Marília B.G.

; Freire, Marlene

; Scheinberg, Morton A.

; Toledo, Roberto A.

; Oliveira, Sheila K.F.

; Fernandes, Vander

; Pinheiro, Marcelo M.

; Castro, Glaucio

; Vieira, Walber Pinto

; Baaklini, Cesar Emile

; Ruffino-Netto, Antonio

; Pinheiro, Geraldo da Rocha Castelar

; Laurindo, Ieda Maria Magalhães

; Louzada-Junior, Paulo

Revista Brasileira de Reumatologia

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2017, Volume 57 Supl. 2 Páginas s477 - s483

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Objetivos Avaliar incidência de tuberculose e triagem para tuberculose latente em brasileiros com artrite reumatoide em uso de agentes biológicos na prática clinica. Pacientes e métodos Estudo de coorte com dados do Registro Brasileiro de Monitoração de Terapias Biológicas (BiobadaBrasil), de 01/2009 a 05/2013, abrangeu 1.552 tratamentos, 415 somente com drogas modificadoras do curso da doença (MMCDs) sintéticas, 942 MMCDs sintéticas em associação com anti-TNF (etanercepte, infliximabe, adalimumabe) e 195 MMCDs sintéticas em associação com outros biológicos (abatacepte, rituximabe e tocilizumabe). Avaliaram-se ocorrência de tuberculose, tempo de exposição às drogas e triagem para TB. Análise estatística: teste t não pareado e teste de Fisher bicaudal; p < 0,05. Resultados O tempo de exposição dos controles foi de 981 pacientes-ano, do grupo de anti-TNF foi de 1.744 pacientes-ano (adalimumabe = 676, infliximabe = 547 e etanercepte = 521 pacientes-ano) e o de outros biológicos de 336 pacientes-ano. A incidência de TB foi de 1,01/1.000 pacientes-ano nos controles e de 2,87 pacientes-ano nos usuários de anti-TNF (adalimumabe = 4,43/1.000 pacientes-ano; etanercepte = 1,92/1.000 pacientes-ano e infliximabe = 1,82/1.000 pacientes-ano). Não houve casos de tuberculose no grupo de outros biológicos. O tempo médio de exposição até a ocorrência de tuberculose foi de 27(11) meses para o grupo anti-TNF. Conclusões A incidência de tuberculose foi maior nos usuários de MMCDs sintéticas e anti-TNF do que nos usuários de MMCDs sintéticas e de MMCDs sintéticas e biológicos não anti-TNF, e também mais tardia, sugerindo infecção durante o tratamento, e não falha na triagem.

Abstract Objectives To assess the incidence of tuberculosis and to screen for latent tuberculosis infection among Brazilians with rheumatoid arthritis using biologics in clinical practice. Patients and methods This cohort study used data from the Brazilian Registry of Biological Therapies in Rheumatic Diseases (Registro Brasileiro de Monitoração de Terapias Biológicas - BiobadaBrasil), from 01/2009 to 05/2013, encompassing 1552 treatments, including 415 with only synthetic disease-modifying anti-rheumatic drugs, 942 synthetic DMARDs combined with anti-tumor necrosis factor (etanercept, infliximab, adalimumab) and 195 synthetic DMARDs combined with other biologics (abatacept, rituximab and tocilizumab). The occurrence of tuberculosis and the drug exposure time were assessed, and screening for tuberculosis was performed. Statistical analysis: Unpaired t-test and Fisher's two-tailed test; p < 0.05. Results The exposure times were 981 patient-years in the controls, 1744 patient-years in the anti-TNF group (adalimumab = 676, infliximab = 547 and etanercept = 521 patient-years) and 336 patient-years in the other biologics group. The incidence rates of tuberculosis were 1.01/1000 patient-years in the controls and 2.87 patient-years among anti-TNF users (adalimumab = 4.43/1000 patient-years; etanercept = 1.92/1000 patient-years and infliximab = 1.82/1000 patient-years). No cases of tuberculosis occurred in the other biologics group. The mean drug exposure time until the occurrence of tuberculosis was 27(11) months for the anti-TNF group. Conclusions The incidence of tuberculosis was higher among users of synthetic DMARDs and anti-TNF than among users of synthetic DMARDs and synthetic DMARDs and non-anti-TNF biologics and also occurred later, suggesting infection during treatment and no screening failure.

https://doi.org/10.1016/j.rbre.2017.05.005 3602 downloads

14.

Recommendations of the Brazilian Society of Rheumatology for the diagnosis and treatment of chikungunya fever. Part 2 - Treatment
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Marques, Claudia Diniz Lopes

; Duarte, Angela Luzia Branco Pinto

; Ranzolin, Aline

; Dantas, Andrea Tavares

; Cavalcanti, Nara Gualberto

; Gonçalves, Rafaela Silva Guimarães

; Rocha Junior, Laurindo Ferreira da

; Valadares, Lilian David de Azevedo

; Melo, Ana Karla Guedes de

; Freire, Eutilia Andrade Medeiros

; Teixeira, Roberto

; Neto, Francisco Alves Bezerra

; Medeiros, Marta Maria das Chagas

; Carvalho, Jozélio Freire de

; Santos, Mario Sergio F.

; Océa, Regina Adalva de L. Couto

; Levy, Roger A.

; Andrade, Carlos Augusto Ferreira de

; Pinheiro, Geraldo da Rocha Castelar

; Abreu, Mirhelen Mendes

; Verztman, José Fernando

; Merenlender, Selma

; Ribeiro, Sandra Lucia Euzebio

; Costa, Izaias Pereira da

; Pileggi, Gecilmara

; Trevisani, Virginia Fernandes Moça

; Lopes, Max Igor Banks

; Brito, Carlos

; Figueiredo, Eduardo

; Queiroga, Fabio

; Feitosa, Tiago

; Tenório, Angélica da Silva

; Siqueira, Gisela Rocha de

; Paiva, Renata

; Vasconcelos, José Tupinambá Sousa

; Christopoulos, Georges

Revista Brasileira de Reumatologia

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2017, Volume 57 Supl. 2 Páginas s438 - s451

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo A febre chikungunya tem se tornado um importante problema de saúde pública nos países onde ocorrem as epidemias, visto que metade dos casos evolui com artrite crônica, persistente e incapacitante. Os dados na literatura sobre terapêuticas específicas nas diversas fases da artropatia ocasionada pela infecção pelo vírus chikungunya (CHIKV) são limitados, não existem estudos randomizados de qualidade que avaliem a eficácia das diferentes terapias. Há algumas poucas publicações sobre o tratamento das manifestações musculoesqueléticas da febre chikungunya, porém com importantes limitações metodológicas. Os dados atualmente disponíveis não permitem conclusões favoráveis ou contrárias a terapêuticas específicas, bem como uma adequada avaliação quanto à superioridade entre as diferentes medicações empregadas. O objetivo deste trabalho foi elaborar recomendações para o tratamento da febre chikungunya no Brasil. Foi feita uma revisão da literatura com seleção de artigos baseados em evidência, nas bases de dados Medline, SciELO, PubMed e Embase e de resumos de anais de congressos, além da opinião dos especialistas para dar apoio às decisões tomadas para definir as recomendações. Para a definição do grau de concordância foi feita uma metodologia Delphi, em duas reuniões presenciais e várias rodadas de votação on line. Este artigo refere-se à parte 2 das Recomendações da Sociedade Brasileira de Reumatologia para Diagnóstico e Tratamento da Febre Chikungunya, que trata especificamente do tratamento.

Abstract Chikungunya fever has become an important public health problem in countries where epidemics occur because half of the cases progress to chronic, persistent and debilitating arthritis. Literature data on specific therapies at the various phases of arthropathy caused by chikungunya virus (CHIKV) infection are limited, lacking quality randomized trials assessing the efficacies of different therapies. There are a few studies on the treatment of musculoskeletal manifestations of chikungunya fever, but these studies have important methodological limitations. The data currently available preclude conclusions favorable or contrary to specific therapies, or an adequate comparison between the different drugs used. The objective of this study was to develop recommendations for the treatment of chikungunya fever in Brazil. A literature review was performed via evidence-based selection of articles in the databases Medline, SciELO, PubMed and Embase and conference proceedings abstracts, in addition to expert opinions to support decision-making in defining recommendations. The Delphi method was used to define the degrees of agreement in 2 face-to-face meetings and several online voting rounds. This study is part 2 of the Recommendations of the Brazilian Society of Rheumatology (Sociedade Brasileira de Reumatologia - SBR) for the Diagnosis and Treatment of chikungunya fever and specifically addresses treatment.

https://doi.org/10.1016/j.rbre.2017.06.004 38509 downloads

15.

Recommendations of the Brazilian Society of Rheumatology for diagnosis and treatment of Chikungunya fever. Part 1 - Diagnosis and special situations
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Marques, Claudia Diniz Lopes

; Duarte, Angela Luzia Branco Pinto

; Ranzolin, Aline

; Dantas, Andrea Tavares

; Cavalcanti, Nara Gualberto

; Gonçalves, Rafaela Silva Guimarães

; Rocha Junior, Laurindo Ferreira da

; Valadares, Lilian David de Azevedo

; Melo, Ana Karla Guedes de

; Freire, Eutilia Andrade Medeiros

; Teixeira, Roberto

; Bezerra Neto, Francisco Alves

; Medeiros, Marta Maria das Chagas

; Carvalho, Jozélio Freire de

; Santos, Mario Sergio F.

; Océa, Regina Adalva de L. Couto

; Levy, Roger A.

; Andrade, Carlos Augusto Ferreira de

; Pinheiro, Geraldo da Rocha Castelar

; Abreu, Mirhelen Mendes

; Verztman, José Fernando

; Merenlender, Selma

; Ribeiro, Sandra Lucia Euzebio

; Costa, Izaias Pereira da

; Pileggi, Gecilmara

; Trevisani, Virginia Fernandes Moça

; Lopes, Max Igor Banks

; Brito, Carlos

; Figueiredo, Eduardo

; Queiroga, Fabio

; Feitosa, Tiago

; Tenório, Angélica da Silva

; Siqueira, Gisela Rocha de

; Paiva, Renata

; Vasconcelos, José Tupinambá Sousa

; Christopoulos, Georges

Revista Brasileira de Reumatologia

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2017, Volume 57 Supl. 2 Páginas s421 - s437

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Resumo A febre chikungunya tem se tornado um importante problema de saúde pública nos países onde ocorrem as epidemias. Até 2013, as Américas haviam registrado apenas casos importados quando, em outubro desse mesmo ano, foram notificados os primeiros casos na Ilha de Saint Martin, no Caribe. No Brasil, os primeiros relatos autóctones foram confirmados em setembro de 2014 e até a semana epidemiológica 37 de 2016 já haviam sido registrados 236.287 casos prováveis de infecção pelo chikungunya vírus (CHIKV), 116.523 confirmados sorologicamente. As mudanças ambientais causadas pelo homem, o crescimento urbano desordenado e o número cada vez maior de viagens internacionais têm sido apontados como os fatores responsáveis pela reemergência de epidemias em grande escala. Caracterizada clinicamente por febre e dor articular na fase aguda, em cerca de metade dos casos existe evolução para a fase crônica (além de três meses), com dor persistente e incapacitante. O objetivo deste trabalho foi elaborar recomendações para diagnóstico e tratamento da febre chikungunya no Brasil. Para isso, foi feita revisão da literatura nas bases de dados Medline, SciELO e PubMed, para dar apoio às decisões tomadas para definir as recomendações. Para a definição do grau de concordância foi feita uma metodologia Delphi, em duas reuniões presenciais e várias rodadas de votação on line. Foram geradas 25 recomendações, divididas em três grupos temáticos: (1) diagnóstico clínico, laboratorial e por imagem; (2) situações especiais e (3) tratamento. Na primeira parte estão os dois primeiros temas e o tratamento na segunda.

Abstract Chikungunya fever has become a relevant public health problem in countries where epidemics occur. Until 2013, only imported cases occurred in the Americas, but in October of that year, the first cases were reported in Saint Marin island in the Caribbean. The first autochthonous cases were confirmed in Brazil in September 2014; until epidemiological week 37 of 2016, 236,287 probable cases of infection with Chikungunya virus had been registered, 116,523 of which had serological confirmation. Environmental changes caused by humans, disorderly urban growth and an ever-increasing number of international travelers were described as the factors responsible for the emergence of large-scale epidemics. Clinically characterized by fever and joint pain in the acute stage, approximately half of patients progress to the chronic stage (beyond 3 months), which is accompanied by persistent and disabling pain. The aim of the present study was to formulate recommendations for the diagnosis and treatment of Chikungunya fever in Brazil. A literature review was performed in the MEDLINE, SciELO and PubMed databases to ground the decisions for recommendations. The degree of concordance among experts was established through the Delphi method, involving 2 in-person meetings and several online voting rounds. In total, 25 recommendations were formulated and divided into 3 thematic groups: (1) clinical, laboratory and imaging diagnosis; (2) special situations; and (3) treatment. The first 2 themes are presented in part 1, and treatment is presented in part 2.

https://doi.org/10.1016/j.rbre.2017.05.006 37996 downloads

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