ABSTRACT When legumes grow associated with other species, they are capable of increasing the total nutritional value of non-nitrogen fixing species as well as their productiv- ity. The objective of this work was to evaluate the biological Nitrogen (N) fixation of Adesmia bicolor (Leguminosae) growing in association with Festuca arundinacea (Gramineae) throughout its life cycle. The trial was carried out at the National University of Río Cuarto. Four treatments were evaluated: A1:F0 (Control I: A. bicolor, pure culture), A1:F1 (A. bicolor and F. arundinacea in similar proportions), A3:F1 (A. bicolor and F. arundinacea, in a ratio of 3 to 1) and A0:F1 (Control II: F. arundinacea, pure culture). During the growth stages, three plots of 0.25 m2 were selected per treatment. Total dry biomass production, number of nodules and N content were determined. In all stages of A. bicolor growth, a greater production of total dry biomass was observed in the A1:F0 pure culture with respect to associates. In F. arundinacea, a greater production of total dry biomass was noted in the pure culture A0:F1 in the vegetative and fruiting stages, while at the flowering stage it was higher in the associated treatments. N content in the two species was higher in the aerial part than in the underground part. The highest N values were recorded in treatment A3:F1. In A. bicolor, the highest production of nodules was detected at the vegetative stage in all treatments. It is concluded that A. bicolor is effective in fixing N both in pure culture and in association. The biomass values of F. arundinacea and N content in both species showed promising results in the association treatments. Further studies should be conducted to understand their behavior.

RESUMEN Las leguminosas al crecer asociadas a otras especies son capaces de incrementar el va- lor nutritivo total de las no fijadoras de Nitrógeno (N) y aumentar su productividad. El objetivo fue evaluar la fijación biológica de N de Adesmia bicolor (Leguminosae) creciendo consociada con Festuca arundinacea (Gramineae) a lo largo de su ciclo de vida. El ensayo se realizó en la Universidad Nacional de Río Cuarto. Se evaluaron cuatro tratamientos: A1:F0 (Control I: A. bicolor, cultivo puro), A1:F1 (A. bicolor y F. arundinacea en proporciones similares), A3:F1 (A. bicolor y F. arundinacea en pro- porciones 3 a 1) y A0:F1 (Control II: F. arundinacea, cultivo puro). Durante las fases de crecimiento se seleccionaron tres áreas de superficie de 0,25 m2 por tratamiento, se determinó producción de biomasa seca total, número de nódulos y contenido de N. En A. bicolor se observó una mayor producción de biomasa seca total en el cultivo puro A1:F0 con respecto a los consociados en todas las fases de crecimiento. En F. arundinacea se notó una mayor producción de biomasa seca total en el cultivo puro A0:F1 en etapa vegetativa y de fructificación, mientras que en etapa de floración fue mayor en los tratamientos consociados. El contenido de N en las dos especies fue más elevado en la parte aérea que subterránea. Los valores más altos de N se presentaron en el tratamiento A3:F1. En A. bicolor la mayor producción de nódulos fue, en todos los tratamientos, en etapa vegetativa. Se concluye que A. bicolor es efectiva para fijar N tanto en cultivo puro como consociada. Teniendo en cuenta los valores de biomasa de F. arundinacea y el contenido de N en ambas especies se observaron resultados prometedores en los tratamientos consociados, por lo cual se sugiere continuar con estudios que permitan comprender el comportamiento de esta asociación.

ABSTRACT When legumes grow associated with other species, they are capable of increasing the total nutritional value of non-nitrogen fixing species as well as their productiv- ity. The objective of this work was to evaluate the biological Nitrogen (N) fixation of Adesmia bicolor (Leguminosae) growing in association with Festuca arundinacea (Gramineae) throughout its life cycle. The trial was carried out at the National University of Río Cuarto. Four treatments were evaluated: A1:F0 (Control I: A. bicolor, pure culture), A1:F1 (A. bicolor and F. arundinacea in similar proportions), A3:F1 (A. bicolor and F. arundinacea, in a ratio of 3 to 1) and A0:F1 (Control II: F. arundinacea, pure culture). During the growth stages, three plots of 0.25 m2 were selected per treatment. Total dry biomass production, number of nodules and N content were determined. In all stages of A. bicolor growth, a greater production of total dry biomass was observed in the A1:F0 pure culture with respect to associates. In F. arundinacea, a greater production of total dry biomass was noted in the pure culture A0:F1 in the vegetative and fruiting stages, while at the flowering stage it was higher in the associated treatments. N content in the two species was higher in the aerial part than in the underground part. The highest N values were recorded in treatment A3:F1. In A. bicolor, the highest production of nodules was detected at the vegetative stage in all treatments. It is concluded that A. bicolor is effective in fixing N both in pure culture and in association. The biomass values of F. arundinacea and N content in both species showed promising results in the association treatments. Further studies should be conducted to understand their behavior.

RESUMEN Las leguminosas al crecer asociadas a otras especies son capaces de incrementar el va- lor nutritivo total de las no fijadoras de Nitrógeno (N) y aumentar su productividad. El objetivo fue evaluar la fijación biológica de N de Adesmia bicolor (Leguminosae) creciendo consociada con Festuca arundinacea (Gramineae) a lo largo de su ciclo de vida. El ensayo se realizó en la Universidad Nacional de Río Cuarto. Se evaluaron cuatro tratamientos: A1:F0 (Control I: A. bicolor, cultivo puro), A1:F1 (A. bicolor y F. arundinacea en proporciones similares), A3:F1 (A. bicolor y F. arundinacea en pro- porciones 3 a 1) y A0:F1 (Control II: F. arundinacea, cultivo puro). Durante las fases de crecimiento se seleccionaron tres áreas de superficie de 0,25 m2 por tratamiento, se determinó producción de biomasa seca total, número de nódulos y contenido de N. En A. bicolor se observó una mayor producción de biomasa seca total en el cultivo puro A1:F0 con respecto a los consociados en todas las fases de crecimiento. En F. arundinacea se notó una mayor producción de biomasa seca total en el cultivo puro A0:F1 en etapa vegetativa y de fructificación, mientras que en etapa de floración fue mayor en los tratamientos consociados. El contenido de N en las dos especies fue más elevado en la parte aérea que subterránea. Los valores más altos de N se presentaron en el tratamiento A3:F1. En A. bicolor la mayor producción de nódulos fue, en todos los tratamientos, en etapa vegetativa. Se concluye que A. bicolor es efectiva para fijar N tanto en cultivo puro como consociada. Teniendo en cuenta los valores de biomasa de F. arundinacea y el contenido de N en ambas especies se observaron resultados prometedores en los tratamientos consociados, por lo cual se sugiere continuar con estudios que permitan comprender el comportamiento de esta asociación.

Resumo Manjericão (Ocimum basilicum) é uma planta medicinal, ornamental e aromática, contudo, seu porte pode ser um entrave para a sua comercialização como planta ornamental de vaso. O paclobutrazol (PBZ) é uma substância que pode retardar o crescimento de plantas, através da inibição da síntese de giberelinas. O objetivo deste trabalho foi avaliar o efeito do paclobutrazol na regulação do crescimento e trocas gasosas no manjericão (Ocimum basilicum var. Cinnamon). O experimento foi realizado em delineamento inteiramente casualizado com 5 tratamentos (doses de PBZ: 0; 2,5; 5; 7,5 e 10 mg L-1), com oito repetições. O crescimento (altura de planta, número de folhas, diâmetro de caule, massa seca da folha, do caule, da inflorescência e total), taxas de crescimento (razão de massa da folha, do caule e da inflorescência e quociente de robustez), índices de clorofilas a e b, trocas gasosas (gs, A, E, Ci, WUE, iWUE e iCE) foram avaliados. Paclobutrazol reduziu o crescimento de plantas de manjericão e aumentou o índice de clorofilas, A, gs e WUE. Paclobutrazol pode ser usado para regular o crescimento de plantas de manjericão var. Cinnamon, sem alterar suas características fisiológicas e ornamentais.

Abstract Basil (Ocimum basilicum) is a medicinal, ornamental and aromatic plant, however, its size can be an obstacle to its commercialization as a potted ornamental plant. Paclobutrazol (PBZ) is a substance that can retard plant growth by inhibiting the synthesis of gibberellins. The objective of this work was to evaluate the effect of paclobutrazol on growth regulation and gas exchange of basil (var. Cinnamon). The experiment was carried out in a completely randomized design with five treatments (PBZ doses: 0, 2.5, 5, 7.5 and 10 mg L-1), with eight replicates. Growth (plant height, number of leaves, stem diameter, leaf dry mass, stem dry mass, inflorescence dry mass, and total), growth rates (leaf mass ratio, stem mass ratio, inflorescence mass ratio, and robustness quotient), chlorophyll indices, gas exchange (gs, A, E, Ci, WUE, iWUE and iCE) were evaluated. Paclobutrazol reduced the growth of basil plants and increased the chlorophyll indices, A, gs, and WUE. Paclobutrazol can be used to regulate plant growth of basil plants var. Cinnamon, without altering its physiological and ornamental characteristics.

Abstract Rodríguez-Cravero, J. F.; M. A. Grossi & D. G. Gutiérrez. 2020. Stevia mandonii (Asteraceae, Eupatorieae): First record for the Argentine flora and lectotypification. Darwiniana, nueva serie 8(1): 402-409. Stevia Cav. (Asteraceae, Eupatorieae) includes ca. 235 species distributed from United States of America to Chile and Argentina in South America. During the taxonomic revision of the Argentinian species several specimens belonging to Stevia mandonii Sch. Bip. were found in the province of Jujuy, where it had not been previously cited. In this framework, the goals of the present article are 1) to report the species in Argentina for the first time, 2) to differentiate it among morphologically close species, and 3) to perform a taxonomical analysis based on herbarium specimens, populations in the field, bibliography and type material. Morphology was analyzed by means of optical microscopy and scanning electron microscopy techniques. Stevia mandonii is readily distinguished by its small size of 10-20 cm, rounded leaves and pappus formed by 9-17 bristles with presence of heterocarpy. A detailed morphological description, an illustration, a geographic distribution map, and a key to species of Stevia that occur in Jujuy are provided. The designation of a lectotype for the name Stevia mandonii is properly discussed.

Resumen Rodríguez-Cravero, J. F.; M. A. Grossi & D. G. Gutiérrez. 2020. Stevia mandonii (Asteraceae, Eupatorieae): Primer registro para la flora argentina y lectotipificación. Darwiniana, nueva serie 8(1): 402-409. Stevia Cav. (Asteraceae, Eupatorieae) incluye ca. 235 especies distribuidas de Estados Unidos de América a Chile y Argentina en Sudamérica. Durante la revisión de las especies argentinas se halló material correspondiente a Stevia mandonii Sch. Bip. en el noroeste del país, donde no había sido citada previamente. En este marco, los objetivos del trabajo son 1) registrar por primera vez a S. mandonii para la Argentina, 2) distinguirla de las especies afines morfológicamente y 3) llevar a cabo un estudio taxonómico basado en ejemplares de herbario, poblaciones en el campo, bibliografía e imágenes de alta resolución del material tipo. La morfología se analizó por medio de técnicas de microscopía óptica y microscopía electrónica de barrido. Stevia mandonii se distingue morfológicamente por su tamaño pequeño, de 10-20 cm de alto, hojas ovadas o redondeadas con ápice obtuso y papus formado por 9 a 17 aristas con presencia de heterocarpía. Como resultado se provee una descripción morfológica detallada, una ilustración, un mapa de distribución y una clave para las especies de Stevia presentes en la provincia de Jujuy. Además, se discute la designación de un lectotipo para el nombre Stevia mandonii.

INTRODUCTION: SAFYRE is a new readjustable and minimally invasive sling for the treatment of stress urinary incontinence (SUI). Attempts to restore the normal suburethral hammock using an anatomical approach have been made in recent years. The authors report their experience with this device, which associates the efficacy of slings with readjustability. MATERIAL AND METHODS: A total of 100 consecutive patients with clinical and urodynamic diagnosis of SUI underwent SAFYRE sling procedure. The age range was from 40 to 71, mean age 63 years. Seventy-five patients (75%) presented previous failed anti-incontinence procedures. Physical clinical examination, stress test, pad use and a urodynamic study were performed before the surgery. All the patients presented symptoms of SUI and 30 % also reported mild urgency. RESULTS: The average follow up period was 14 months (12 - 30 months). The mean operative time was of 25 minutes. Dystopia repair was performed whenever necessary, during the same procedure. The average hospital stay was 24 hours. In 3% of the implants, bladder perforation occurred. During the postoperative period, 26 patients developed urgency symptoms. During that follow up period, 92% were found to be continent, 3% reported an improvement and 5% were dissatisfied. CONCLUSION: SAFYRE is a safe and quick procedure that allows for postoperative readjustment. This technique may be an attractive alternative if the good result obtained so far proves to be long lasting.

INTRODUCCIÓN: SAFYRE es un nuevo cabestrillo (dispositivo de suspensión) reajustable y mínimamente invasivo para el tratamiento de la incontinencia urinaria por estrés (IUE). En los últimos años se ha intentado restaurar el lecho suburetral normal utilizando una técnica anatómica. Los autores comunican su experiencia con este dispositivo, que además de poseer la eficacia de los cabestrillos tiene capacidad de reajustación. MATERIALES y MÉTODOS: Un total de 100 pacientes consecutivas con diagnóstico de IUE desde el punto de vista clínico y urodinámico se sometieron al procedimiento de implantación del cabestrillo SAFYRE. Su rango de edad era de 40 a 71 años, con una edad media de 63 años. Previamente, 75 pacientes (75%) se habían sometido a procedimientos para la incontinencia que habían fracasado. Antes de la cirugía se realizaron una exploración física clínica, una prueba de estrés, un estudio urodinámico y un estudio sobre el uso de almohadillas. Todas las pacientes presentaban síntomas de IUE y el 30% también tenían tenesmo leve. RESULTADOS: El periodo de seguimiento medio fue de 14 meses (12 - 30 meses). El tiempo medio de la operación fue de 25 minutos. Durante el mismo procedimiento se reparó la distopia cuando se consideró necesario. La estancia hospitalaria media fue de 24 horas. En el 3% de los implantes se produjo perforación de la vejiga. Durante el periodo posoperatorio 26 pacientes presentaron síntomas de tenesmo. Durante ese periodo de seguimiento, el 92% presentaron continencia urinaria, el 3% mejoraron y el 5% no estuvieron satisfechas con el procedimiento. CONCLUSIÓN: SAFYRE es un procedimiento seguro y rápido que permite el reajuste posoperatorio. Esta técnica puede ser una alternativa atractiva si se demuestra que los buenos resultados obtenidos hasta ahora son de larga duración.

Ensaios de campo foram conduzidos por dois anos, com a finalidade de verificar se a amostragem comumente feita (usual) em canteiros experimentais de algodão, retirando-se a amostra do terço médio, é representativa de toda a planta. No presente estudo esta avaliação foi feita analisando as características de comprimento da fibra, índice de finura (Micronaire), uniformidade de comprimento, índice de resistência (Pressley), porcentagem de fibras e peso de um capulho, sendo os dados obtidos para a amostra usual correlacionados com os dados de amostras marcadas, constituídas por capulhos de mesma idade, provenientes de flores abertas no mesmo dia e, portanto, sujeitas às mesmas condições climáticas. Tais capulhos foram colhidos de diferentes partes da planta, isto é, do baixeiro, da parte mediana e do ponteiro. Os resultados revelaram que a média das amostras marcadas apresentou boa correlação com a amostra da parte mediana da planta, indicando ser o terço médio representativo da planta. A amostra usual apresentou melhor correlação com a média das amostras marcadas. Observou-se também que a amostra usual pode, em determinadas condições, fornecer melhores conclusões que a média das amostras marcadas, sendo prática e possuindo boa precisão.

The technological properties of cotton fibers, such as upper half mean length, length uniformity, strength, and Micronaire fineness, and other characteristics such as percent lint and weight of one boll were studied in relation to four sampling methods, to evaluate whether the usual sampling procedure from the medium third part of the peant is representative of all the plant. The data obtained for usual sampling procedure were correlated with results from marked samples. This samples were composed by bolls of same age, obtained from flowers opened in the same day and subjected to the same weather conditions. Those bolls were harvested from the following parts of the plant: (i) lower, (ii) medium, and (iii) upper section. The results showed that the average of marked samples gave good correlation with the sample from medium section (ii), indicating to be the medium third representative of all the plant. The samples obtained by usual procedures gave better correlation with the average of marked samples. Also it was shown that the usual procedure may give better results than the average of marked samples, being more practical and with good precision.

Neste trabalho são descritos os métodos analíticos empregados para a determinação de micronutrientes (Fe, Mn, Cu, Zn e Mo), cobalto, fósforo orgânico e inorgânico, silica, matéria orgânica e carbono orgânico em torta de filtro rotativo. São apresentadas as marchas analíticas preconizadas e os resultados obtidos nas determinações efetuadas em vinte e duas amostras do material.

This paper describe the analytical methods employed for the determination of copper, zinc, iron, manganese, cobalt, molybdenum, organic and organic phosphorus, silicon, organic mater and organic carbon in filter mud.