Abstract Background Eragrostis plana is the main invasive plant in the Pampa Biome of southern Brazil. This plant is highly competitive, tolerant to abiotic stresses, and very difficult to manage. Plants exposed to abiotic stresses and herbicides develop mechanisms that help alleviate or reduce damage caused by stressors and transmit this ability to the progenies. Objective The study’s objectives were to ascertain whether acclimatization to stress due to drought and sub-lethal doses of glyphosate may decrease plant sensitivity to glyphosate and investigate the possible memory mechanisms involved in this process. Methods A population of E. plana was submitted to drought, glyphosate, or a combination of drought plus glyphosate for two generations. The progenies were analyzed for sensitivity to the herbicide glyphosate and for biochemical, metabolites, and molecular responses. Results When testing sensitivity to glyphosate in the G 2 generations, the DRYxGLY (plants stressed with drought plus glyphosate) was the least sensitive (ED50 443.0 g a.e. ha-1), while CHK (without stresses) was the most sensitive (ED50 278.0 g a.e. ha-1). Evaluating the effects of glyphosate, the CHK population showed a greater accumulation of oxidative damage and shikimic acid. While DRYxGLY had greater antioxidant activity and higher expressions of the EPSPS and ABC-carrier MRP10 genes. Conclusions Recurrent selection with drought stress and sub-lethal rate of glyphosate (DRYxGLY) showed reduced sensitivity to glyphosate in the second generation ( G 2 ). The conjunction of factors, including the upregulation of EPSPS and the ABC MRP10 transporter, antioxidant enzymes seem to decrease the sensitivity of the DRYxGLY population to glyphosate.

Abstract Background: Clearfield® (CL) rice is one of the most successful tools for selective weedy rice control, using imidazolinone herbicides (IMI). However, IMI have residual soil activity and may carryover to non-tolerant crops growing in succession. It is necessary to find options to reduce IMI persistence in paddy rice fields. Objective: Evaluate the effect of water management on IMI dissipation in rice paddy and its carryover to soybean and non-CL rice. Methods: The herbicide mixture of imazapyr and imazapic totalizing 147 g a.i. ha−1 and 49 g a.i. ha−1, respectively, was applied half in preemergence (S3 stage – rice spike stage) and the other half in postemergence (V3-V4 rice stage). After herbicide application, a total of 21 samples were taken for analysis of residues. Soil samples were air dried and herbicides residues were analyzed using high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Results: Calculated field dissipation half-life (DT½) of imazapyr was 182.5 and 42.0 days in continuous flooding and intermittent water management, respectively. Imazapic had a DT½ of 96.3 days on intermittent water management. No adjustment for the dissipation regression parameters was obtained for imazapic in continuous water management. No reduction in yield components was observed in soybean when grown in soil with imidazolinone residues. On the other hand, non-CL rice showed decreased shoot dry weight and stem number. Conclusion: Imidazolinone herbicides have a longer half-life under continuous rice water management than intermittent. Therefore, growers can use intermittent irrigation in ares prone to IMI carryover and can use soybean in rotation.